CN112806485A - Compound feed for relieving stress of lambs and preparation method and application thereof - Google Patents
Compound feed for relieving stress of lambs and preparation method and application thereof Download PDFInfo
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- CN112806485A CN112806485A CN202011600219.7A CN202011600219A CN112806485A CN 112806485 A CN112806485 A CN 112806485A CN 202011600219 A CN202011600219 A CN 202011600219A CN 112806485 A CN112806485 A CN 112806485A
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- stress
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- 241000283903 Ovis aries Species 0.000 title claims description 52
- 238000002360 preparation method Methods 0.000 title claims description 10
- 230000035882 stress Effects 0.000 claims abstract description 94
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 34
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 34
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 29
- 235000019687 Lamb Nutrition 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 25
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000006041 probiotic Substances 0.000 claims abstract description 20
- 235000018291 probiotics Nutrition 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 15
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 15
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 15
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- 238000002156 mixing Methods 0.000 claims description 31
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 30
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 15
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- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 14
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 14
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 14
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- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 14
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 14
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- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 14
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- 239000011591 potassium Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention specifically provides a compound feed for relieving lamb stress, which adopts various mineral elements and vitamins as raw materials, adopts bacillus natto, bacillus subtilis and lactic acid bacteria to be compounded into compound probiotics combined with yeast culture, probiotics, trehalose and skim milk powder, has better effects on improving and solving transition stress, material changing stress, weaning stress, immune stress, management stress and immune stress and preventing enterotoxemia caused by clostridium welchii, has short feeding transition time, has the advantages that the feed intake and weight increase after transition are not less than 20 percent compared with those of a control group, and the compound feed does not need to be supplemented with pasture, can be used by being matched with conventional feed, can obviously reduce the feeding cost, is safe and reliable, and has wide development value for lamb breeding.
Description
Technical Field
The invention belongs to the technical field of feed processing, relates to a preparation method of a lamb feed, and particularly relates to a compound feed for relieving stress of lambs, and a preparation method and an application thereof.
Background
The stress response of sheep refers to a series of neuroendocrine responses mainly caused by sympathetic excitation and increased pituitary-adrenal cortex secretion, and thus various functional and metabolic changes of sheep, wherein the neuroendocrine responses are caused by rapid increase of blood adrenocorticotropic hormone concentration and massive glucocorticoid secretion through hypothalamus when the sheep are only strongly stimulated. The physiological responses to stress are manifested by sympathetic stimulation, increased secretion of pituitary and adrenal cortical hormones, elevated blood glucose, elevated blood pressure, increased heart rate, and accelerated respiration. Stress may also lead to some deficiency of sodium, potassium, chlorine, calcium, magnesium and other trace elements in sheep.
Stress reaction is the adaptive reaction of sheep body for maintaining life activity, which has no infectivity but has seasonality, and abnormal and undesirable stress factors appearing in the feeding or production environment may cause the sheep to produce the non-specific and physiological stress state; stress in sheep is sometimes localized, such as inflammation. Sometimes systemic, such as sheep systemic indication syndrome. In the production practice of sheep raising industry, the stressors causing the stress response almost cover the whole process of sheep raising production. However, if the stress is too intense or too long lasting, whether physiological or psychological, it will be detrimental, causing less discomfort to the sheep's body and more so, a barrier to sheep production performance.
Aiming at goats in a Xinjiang pastoral area, a plurality of factors generating stress exist in the goat with grass fat at present: as the lambs adapt to the mountain stocking environment, transition stress exists after the lambs are driven off the mountain; the method is characterized in that the method is to graze on the grassland, the method is switched to the colony house for cultivation, the cultivation density is changed violently, and management stress exists; when the grass is eaten to be changed into the feed, the stress of material change exists; after going down the hill, the lamb is changed from eating milk and grass to eating feed, and weaning stress exists; because immune programs such as triple four-prevention, small rumination, sheep pox, foot-and-mouth disease and the like are carried out after the lambs leave the mountains, the lamb immune stress is larger, and the general fat condition of the lambs does not rise or fall reversely at the stage; due to the various stresses and too fast reloading, the large proliferation of the rumen clostridium welchii is caused, and enterotoxemia, diarrhea or lamb death is caused finally.
In the prior art, the invention patent with the application number of 201911290567.6 discloses 'a nutritional feed set for early weaning of Tibetan lambs under grazing conditions and a method for early weaning of Tibetan lambs', the invention patent with the application number of 201910504596.1 provides 'a fermented compound feed for early fattening lambs', the invention patent with the application number of 201410504092.7 provides 'a total mixed ration feed for reducing weaning stress of lambs', the invention patent with the application number of 201911084924.3 provides 'a biological feed for relieving the slaughtering stress of sheep and a preparation method thereof', the invention patent with the application number of 201911140374.2 provides 'a ruminant anti-stress feed prepared by fermenting jujube powder', the invention patent with the application number of 201911368889.8 'a compound premixed feed for cattle and sheep', and the invention patent with the application number of 201510977696.8 'a Hu sheep raising set application method and a feed', which solve the stress caused by single factor, such as weaning stress, stress in the transportation process and the like, and needs to be separately configured when in use, so that the application range is small.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the compound feed for relieving the stress of the lambs, and the compound feed has better effects on improving and solving transition stress, reloading stress, weaning stress, immune stress, management stress and immune stress and preventing enterotoxemia caused by clostridium welchii. For the lower-mountain grass fat sheep within 30kg, the feeding transition time is short, the feed intake and the weight increase after transition are not less than 20% compared with those of a control group, and the feed does not need to be supplemented with pasture, and the feed can be used by being matched with conventional feed, so that the feeding cost can be obviously reduced, and the feed has wide development value for lamb breeding.
The invention is realized by the following technical scheme:
the invention provides a compound feed for relieving lamb stress, which comprises 8.1-9.7% of ferrous sulfate, 0.8-1.6% of copper sulfate, 3.62-7.52% of manganese sulfate, 11-15% of zinc sulfate, 19.7-21.7% of yeast selenium, 0.4% of potassium iodide, 3.94-5.54% of calcium carbonate, 7.8-9.8% of vitamin A, 7.7-8.9% of vitamin D, 12.8-14.8% of vitamin E, 3-7% of yeast culture, 4.9-6.9% of probiotics, 2% of trehalose and 1.8-2.2% of skimmed milk powder by weight.
Preferably, the compound feed for alleviating stress of lambs comprises, by weight, 8.90% of ferrous sulfate, 1.20% of copper sulfate, 5.62% of manganese sulfate, 13.00% of zinc sulfate, 20.70% of yeast selenium, 0.04% of potassium iodide, 4.74% of calcium carbonate, 8.80% of vitamin A, 8.30% of vitamin D, 13.80% of vitamin E, 5.00% of yeast culture, 5.90% of probiotics, 2% of trehalose and 2% of skimmed milk powder.
Wherein, the vitamin A is 500000IU/g, the vitamin D is 500000IU/g, and the vitamin E is 500 IU/g.
Further, the invention specifically provides a preparation method of the compound feed for relieving stress of lambs, and the specific production steps are as follows:
(1) removing impurities from ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, selenium yeast, potassium iodide and calcium carbonate, and sieving with 80-100 mesh sieve;
(2) selecting Bacillus natto (Bacillus natto), Bacillus subtilis and lactobacillus (Lactobacillus acid bacteria) freeze-dried powder according to the mass ratio of (3-7): (7-9): 2 mixing, performing amplification culture with beef extract peptone culture medium with pH of 6.8-7.6, culture temperature of 31-39 deg.C, culture time of 17-33h, and determining viable countNot less than 2X 108cfu/g；
(3) Freeze-drying the composite probiotics prepared in the step (2), and mixing with trehalose and skimmed milk powder;
(4) mixing vitamin A, vitamin D, vitamin E and yeast culture uniformly;
(5) mixing the materials prepared in the steps (1), (3) and (4), and packaging the mixture by adopting a feed packaging bag provided with a one-way valve;
preferably, the probiotics are prepared from Bacillus natto (Bacillus natto), Bacillus subtilis and Lactic acid bacteria (Lactic acid bacteria) according to a mass ratio of 5: 9: 2, compounding to obtain the product.
Preferably, the fermentation time is 25 h.
Preferably, the fermentation temperature is 35 ℃, and the optimal pH is 7.2.
The invention provides the compound feed for relieving the stress of lambs, which is obtained by the preparation process.
Furthermore, the invention also provides application of the compound feed for relieving stress of the lambs in relieving the stress of the lambs.
Preferably, the compound feed for relieving the stress of the lambs is uniformly mixed with the crushed energy feed and the protein feed to obtain the daily ration, and the compound feed for relieving the stress of the lambs accounts for 4% of the total weight of the daily ration.
Preferably, the compound feed for alleviating stress of lambs is used for alleviating transition stress, feed changing stress, weaning stress, immune stress, management stress, immune stress and preventing enterotoxemia caused by clostridium welchii.
The Bacillus natto, the Bacillus subtilis and the lactic acid bacteria are harmless and safe strains allowed by the agricultural department, are all purchased from China general microbiological culture Collection center (CGMCC), and can be obtained by common technicians through public channels, and the culture conditions and the culture media of the four strains can be obtained by common reports in the field.
By implementing the specific invention content of the invention, the following beneficial effects can be achieved:
(1) by adopting the compound feed for relieving the stress of the lambs, the compound feed has better effects on improving and solving transition stress, reloading stress, weaning stress, immune stress, management stress and immune stress and preventing enterotoxemia caused by clostridium welchii. For the lower-mountain grass fat sheep within 30kg, the feeding transition time is short, the feed intake and the weight increase after transition are not lower than 20% compared with those of a control group, and the feed does not need to be supplemented with pasture, and the feed can be used by being matched with conventional feed, so that the feeding cost can be obviously reduced, the feed is safe and reliable, and the feed has wide development value for lamb breeding.
(2) According to the feed for improving the muscular stomach development of the broiler chicken of the large lamb, provided by the invention, three strains of Bacillus natto (Bacillus natto), Bacillus subtilis and lactic acid bacteria (lactic acid bacteria) are compounded according to a specific proportion, the constructed composite probiotics can act synergistically to form a stable microecological system, and the adopted strains are all intestinal probiotics, so that the influence of stress on the growth of the lamb and the incidence of enterotoxemia caused by clostridium welchii are reduced to the maximum extent on the premise of ensuring the normal growth of the lamb.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
The materials of the invention are: ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, selenium yeast, potassium iodide, calcium carbonate, vitamin A, vitamin D, vitamin E, yeast culture, probiotics, trehalose and skimmed milk powder. The reagents and materials can be purchased through public channels, and the equipment and instruments adopted in the process are all common equipment in the field.
All materials, reagents and equipment selected for use in the present invention are well known in the art, but do not limit the practice of the invention, and other reagents and equipment well known in the art may be suitable for use in the practice of the following embodiments of the invention.
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention.
The first embodiment is as follows: compound feed for relieving stress of lambs
The invention provides a compound feed for relieving lamb stress, which comprises 8.1-9.7% of ferrous sulfate, 0.8-1.6% of copper sulfate, 3.62-7.52% of manganese sulfate, 11-15% of zinc sulfate, 19.7-21.7% of yeast selenium, 0.4% of potassium iodide, 3.94-5.54% of calcium carbonate, 7.8-9.8% of vitamin A, 7.7-8.9% of vitamin D, 12.8-14.8% of vitamin E, 3-7% of yeast culture, 4.9-6.9% of probiotics, 2% of trehalose and 1.8-2.2% of skimmed milk powder by weight.
Example two: preparation method of compound feed for relieving stress of lambs
The invention specifically provides a preparation method of a compound feed for relieving lamb stress, which comprises the following specific production steps:
(1) removing impurities from ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, selenium yeast, potassium iodide and calcium carbonate, and sieving with 80-100 mesh sieve;
(2) selecting Bacillus natto (Bacillus natto), Bacillus subtilis and lactobacillus (Lactobacillus acid bacteria) freeze-dried powder according to the mass ratio of (3-7): (7-9): 2 mixing, performing amplification culture with beef extract peptone culture medium at pH of 6.8-7.6 and culture temperature of 31-39 deg.C for 17-33h, and determining viable count not less than 2 × 108cfu/g；
(3) Freeze-drying the composite probiotics prepared in the step (2), and mixing with trehalose and skimmed milk powder;
(4) mixing vitamin A, vitamin D, vitamin E and yeast culture uniformly;
(5) mixing the materials prepared in the steps (1), (3) and (4), and packaging the mixture by adopting a feed packaging bag provided with a one-way valve;
example three: compound feed for relieving stress of lambs
Removing impurities from 9.7kg of ferrous sulfate, 1.6kg of copper sulfate, 7.62kg of manganese sulfate, 11kg of zinc sulfate, 19.7kg of yeast selenium, 0.04kg of potassium iodide and 3.94kg of calcium carbonate, and sieving by a sieve of 80-100 meshes; selecting Bacillus natto, Bacillus subtilis and lactobacillus (Lactic acid bacteria) freeze-dried powder according to the mass ratio of 3: 7: 2 mixing, performing amplification culture by adopting beef extract peptone culture medium, wherein the pH of the culture medium is 6.8, the culture temperature is 31 ℃, the culture time is 17h, and the number of viable bacteria is not less than 2 multiplied by 108cfu/g; freeze-drying 4.9kg of the prepared composite probiotics, and mixing with 2kg of trehalose and 2.2kg of skimmed milk powder; mixing 9.8kg of vitamin A, 7.7kg of vitamin D, 8kg of vitamin E and 7kg of yeast culture evenly; mixing the prepared materials, and packaging with a feed packaging bag provided with a one-way valve.
Example four: compound feed for relieving stress of lambs
Removing impurities from 9.3kg of ferrous sulfate, 1.4kg of copper sulfate, 6.62kg of manganese sulfate, 12kg of zinc sulfate, 20.2kg of yeast selenium, 0.04kg of potassium iodide and 4.34kg of calcium carbonate, and sieving with a 80-100-mesh sieve; selecting Bacillus natto, Bacillus subtilis and lactobacillus (Lactic acid bacteria) freeze-dried powder according to the mass ratio of 4: 8: 2 mixing, performing amplification culture with beef extract peptone culture medium at pH 7 and culture temperature 33 deg.C for 21 hr, and determining viable count not less than 2 × 108cfu/g; freeze-drying 5.4kg of the prepared composite probiotics, and mixing with 2kg of trehalose and 2.1kg of skimmed milk powder; mixing vitamin A9.3 kg, vitamin D8. kg, vitamin E13.3kg and yeast culture 6 kg; mixing the prepared materials, and packaging with a feed packaging bag provided with a one-way valve.
Example five: compound feed for relieving stress of lambs
Removing impurities from 8.90kg of ferrous sulfate, 1.2kg of copper sulfate, 5.62kg of manganese sulfate, 13.00kg of zinc sulfate, 20.7kg of yeast selenium, 0.04kg of potassium iodide and 4.74kg of calcium carbonate, and sieving by a sieve of 80-100 meshes; selecting bacillus natto(Bacillus natto), Bacillus subtilis and lactobacillus (Lactic acid bacteria) freeze-dried powder according to the mass ratio of 5: 9: 2 mixing, performing amplification culture by using beef extract peptone culture medium, wherein the pH of the culture medium is 7.2, the culture temperature is 35 ℃, the culture time is 25h, and the number of viable bacteria is not less than 2 multiplied by 108cfu/g; freeze-drying 5.9kg of the prepared composite probiotics, and mixing with 2.0kg of trehalose and 2.0kg of skimmed milk powder; uniformly mixing 8.8kg of vitamin A, 8.3kg of vitamin D, 8.8kg of vitamin E and 5kg of yeast culture; mixing the prepared materials, and packaging with a feed packaging bag provided with a one-way valve.
Example six: compound feed for relieving stress of lambs
Removing impurities from 8.5kg of ferrous sulfate, 1kg of copper sulfate, 4.62kg of manganese sulfate, 14kg of zinc sulfate, 21.2kg of yeast selenium, 0.04kg of potassium iodide and 5.14kg of calcium carbonate, and sieving by a sieve of 80-100 meshes; selecting Bacillus natto, Bacillus subtilis and lactobacillus (Lactic acid bacteria) freeze-dried powder according to the mass ratio of 6: 9: 2 mixing, performing amplification culture by using beef extract peptone culture medium, wherein the pH of the culture medium is 7.4, the culture temperature is 37 ℃, the culture time is 29h, and the number of viable bacteria is not less than 2 multiplied by 108cfu/g; freeze-drying 5.9kg of the prepared composite probiotics, and mixing with 2.0kg of trehalose and 2.0kg of skimmed milk powder; uniformly mixing 8.3kg of vitamin A, 8.6kg of vitamin D, 14.3kg of vitamin E and 4kg of yeast culture; mixing the prepared materials, and packaging with a feed packaging bag provided with a one-way valve.
Example seven: compound feed for relieving stress of lambs
Removing impurities from 8.1kg of ferrous sulfate, 0.8kg of copper sulfate, 3.62kg of manganese sulfate, 15kg of zinc sulfate, 21.7kg of yeast selenium, 0.04kg of potassium iodide and 5.54kg of calcium carbonate, and sieving by a sieve of 80-100 meshes; selecting Bacillus natto, Bacillus subtilis and lactobacillus (Lactic acid bacteria) freeze-dried powder according to the mass ratio of 7: 9: 2 mixing, performing amplification culture by adopting beef extract peptone culture medium, wherein the pH of the culture medium is 7.6, the culture temperature is 39 ℃, the culture time is 33h, and the number of viable bacteria is not less than 2 multiplied by 108cfu/g; to be prepared6.9kg of composite probiotics freeze-dried powder is mixed with 2.0kg of trehalose and 1.8kg of skimmed milk powder; uniformly mixing 7.8kg of vitamin A, 8.9kg of vitamin D, 8.8kg of vitamin E and 3kg of yeast culture; mixing the prepared materials, and packaging with a feed packaging bag provided with a one-way valve.
Example eight: compound feed feeding effect verification for relieving lamb stress
1. Feeding management
The feeding test is carried out in a Xinjiang Taikun group lamb breeding shed, and flat breeding is carried out by adopting ground padding, the technical scheme provided by the third embodiment to the seventh embodiment of the invention is that a test 1-5 groups are adopted, a control group adopts national standard to feed the lambs, each treatment adopts 120 lambs with the similar daily age and weight in the same batch of 30kg, each group comprises 20 lambs, the adopted lambs are fed and managed in the early period and have consistent conditions, and the lambs are fed and drunk freely and are fed and immunized according to the conventional feeding operation rules and immunization procedures. The test periods were as follows: going down a hill and transferring; changing grazing into breeding in a colony house; the milk and grass eating is changed into the feed; immune programs such as triple four-prevention, small ruminants, sheep pox, foot-and-mouth disease and the like; comprises transition stress, management stress, material change stress and immune stress in the feeding process, wherein the stress generation is respectively stage 1, stage 2, segment 3, stage 4 and stage 5. And recording the weight of the stress factors at each stage on the day of generation, adding the compound feed prepared according to the technical scheme of each embodiment into the energy feed and the protein feed, recording the weight after feeding for 10 days, comparing the recorded weight with a control group, and measuring the weight, the feed intake increase condition and the enterotoxemia morbidity condition. And after the experimental period is finished, selecting five sheep from each group for slaughtering, selecting colon tissues, scraping contents, and carrying out D-type clostridium welchii detection by using an ELISA detection kit. The measurement results are shown in table 1.
Table 1: results of feeding effect measurement
According to the determination results, after the compound feed for relieving the lamb stress provided by the invention is adopted, the lamb feeding condition and the weight increasing condition are replaced compared with those of a control group, wherein the compound feed for relieving the lamb stress prepared in the fifth embodiment has the highest weight increase and feed intake increase level. After the compound feed for relieving lamb stress prepared by the technical scheme provided by the invention is adopted, the infection condition of the clostridium welchii in intestinal tracts of lambs is remarkably improved, and the incidence rate of enterotoxemia is remarkably reduced.
Based on the above embodiments, it can be seen that the present application creatively provides a compound feed for alleviating stress of lambs, aiming at the conventional technical prejudice that the prior art only adopts complete formula of specific components for alleviating stress of lambs and can obtain the best stress alleviation effect by feeding in the whole process of lambs growth, the present invention aims to provide a compound feed for alleviating stress of lambs, which can significantly improve the growth condition of the muscular stomach of the lambs, overcome adverse effects of transition stress, material change stress, weaning stress, immunological stress, management stress and immunological stress on the growth of lambs, have good prevention effect on enterotoxemia caused by clostridium welchii, and have no adverse effect on the development of lambs, the prepared compound feed for alleviating stress of lambs is non-toxic and harmless, and is only combined with conventional energy feed and protein feed for short-term feeding when stress factors are generated, the forage grass is not needed to be added additionally, and the operation is simple. The method can maintain the normal growth requirement of the goat fat at the same time of reducing the occurrence of enterotoxemia related diseases, and has great potential development value for the development of the breeding industry of the lambs.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (10)
1. The compound feed for relieving stress of lambs is characterized by comprising, by mass, 8.1% -9.7% of ferrous sulfate, 0.8% -1.6% of copper sulfate, 3.62% -7.52% of manganese sulfate, 11% -15% of zinc sulfate, 19.7% -21.7% of yeast selenium, 0.4% of potassium iodide, 3.94% -5.54% of calcium carbonate, 7.8% -9.8% of vitamin A, 7.7% -8.9% of vitamin D, 12.8% -14.8% of vitamin E, 3% -7% of yeast culture, 4.9% -6.9% of probiotics, 2% of trehalose and 1.8% -2.2% of skimmed milk powder.
2. The compound feed for alleviating stress of lambs according to claim 1, which comprises, by mass, 8.90% of ferrous sulfate, 1.20% of copper sulfate, 5.62% of manganese sulfate, 13.00% of zinc sulfate, 20.70% of yeast selenium, 0.04% of potassium iodide, 4.74% of calcium carbonate, 8.80% of vitamin A, 8.30% of vitamin D, 13.80% of vitamin E, 5.00% of yeast culture, 5.90% of probiotics, 2% of trehalose and 2% of skimmed milk powder.
3. The compound feed for alleviating stress in lambs as claimed in claim 1, wherein the vitamin a is 500000IU/g, the vitamin D is 500000IU/g, and the vitamin E is 500 IU/g.
4. The preparation method of the compound feed for alleviating stress of lambs according to claim 1, which is prepared by the following steps:
(1) removing impurities from ferrous sulfate, copper sulfate, manganese sulfate, zinc sulfate, selenium yeast, potassium iodide and calcium carbonate, and sieving with 80-100 mesh sieve;
(2) selecting Bacillus natto (Bacillus natto), Bacillus subtilis and lactobacillus (Lactobacillus acid bacteria) freeze-dried powder according to the mass ratio of (3-7): (7-9): 2 mixing, performing amplification culture with beef extract peptone culture medium at pH of 6.8-7.6 and culture temperature of 31-39 deg.C for 17-33h, and determining viable count not less than 2×108cfu/g；
(3) Freeze-drying the composite probiotics prepared in the step (2), and mixing with trehalose and skimmed milk powder;
(4) mixing vitamin A, vitamin D, vitamin E and yeast culture uniformly;
(5) mixing the materials prepared in the steps (1), (3) and (4), and packaging the mixture by adopting a feed packaging bag provided with a one-way valve.
5. The method for preparing the compound feed for alleviating stress of lambs according to claim 4, wherein the probiotics comprise Bacillus natto (Bacillus natto), Bacillus subtilis (Bacillus subtilis) and lactobacillus (Lactic acid bacteria) according to a mass ratio of 5: 9: 2, compounding to obtain the product.
6. The method of claim 4, wherein the fermentation time is 25 hours.
7. The method of claim 4, wherein the fermentation temperature is 35 ℃ and the pH optimum is 7.2.
8. Use of a lamb stress relief compound feed according to any one of claims 1 to 7 for the relief of lamb stress.
9. The use of the compound feed for alleviating stress in lambs according to any one of claims 1 to 7 in the alleviation of stress in lambs, wherein the compound feed for alleviating stress in lambs is uniformly mixed with the crushed energy feed and the protein feed to obtain a ration, the compound feed for alleviating stress in lambs accounts for 4% of the total weight of the ration, and the feeding time is 7-10 days.
10. Use of a lamb stress relief compound feed according to any one of claims 1 to 7 for the relief of lamb stress, wherein the compound feed for the relief of lamb stress is used for the relief of transition stress, feed change stress, weaning stress, immune stress, management stress, immune stress and the prevention of enterotoxemia caused by clostridium welchii.
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