CN112724216A - 改变玉米开花期的基因及方法 - Google Patents
改变玉米开花期的基因及方法 Download PDFInfo
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CN202110093475.XA CN112724216B (zh) | 2021-01-22 | 2021-01-22 | 改变玉米开花期的基因及方法 |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050108791A1 (en) * | 2001-12-04 | 2005-05-19 | Edgerton Michael D. | Transgenic plants with improved phenotypes |
CN102906267A (zh) * | 2010-01-06 | 2013-01-30 | 先锋国际良种公司 | 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途 |
US20150143581A1 (en) * | 1999-05-06 | 2015-05-21 | Monsanto Technology Llc | Nucleic acid molecules and other molecules associated with plants and uses thereof |
CN104903444A (zh) * | 2012-10-31 | 2015-09-09 | 日本烟草产业株式会社 | 对植物赋予高产性的核酸、制备产量增加的转基因植物的方法、使植物的产量增大的方法 |
US20170114356A1 (en) * | 2015-02-20 | 2017-04-27 | E I Du Pont De Nemours And Company | Novel alternatively spliced transcripts and uses thereof for improvement of agronomic characteristics in crop plants |
CN111172173A (zh) * | 2020-02-21 | 2020-05-19 | 未米生物科技(江苏)有限公司 | 降低玉米株高或延迟开花的方法 |
-
2021
- 2021-01-22 CN CN202110093475.XA patent/CN112724216B/zh active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150143581A1 (en) * | 1999-05-06 | 2015-05-21 | Monsanto Technology Llc | Nucleic acid molecules and other molecules associated with plants and uses thereof |
US20050108791A1 (en) * | 2001-12-04 | 2005-05-19 | Edgerton Michael D. | Transgenic plants with improved phenotypes |
CN102906267A (zh) * | 2010-01-06 | 2013-01-30 | 先锋国际良种公司 | 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途 |
CN104903444A (zh) * | 2012-10-31 | 2015-09-09 | 日本烟草产业株式会社 | 对植物赋予高产性的核酸、制备产量增加的转基因植物的方法、使植物的产量增大的方法 |
US20170114356A1 (en) * | 2015-02-20 | 2017-04-27 | E I Du Pont De Nemours And Company | Novel alternatively spliced transcripts and uses thereof for improvement of agronomic characteristics in crop plants |
CN111172173A (zh) * | 2020-02-21 | 2020-05-19 | 未米生物科技(江苏)有限公司 | 降低玉米株高或延迟开花的方法 |
Non-Patent Citations (7)
Title |
---|
HAIJUN LIU 等: ""Genomic, Transcriptomic, and Phenomic Variation Reveals the Complex Adaptation of Modern Maize Breeding"", 《MOLECULAR PLANT》 * |
HAI-JUN LIU 等: ""High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize"", 《PLANT CELL》 * |
HAYES,K.R.等: ""Zea mays TOC1b mRNA, partial cds"", 《GENBANK》 * |
MINLIANG JIN 等: ""ZmCOL3, a CCT gene represses flowering in maize by interfering with the circadian clock and activating expression of ZmCCT"", 《JIPB》 * |
QINGBIAO SHI 等: ""Molecular mechanisms governing shade responses in maize"", 《COMMUNICATIONS》 * |
WARE,D.: ""Two-component response regulator-like APRR1 [Zea mays]"", 《GENBANK》 * |
金敏亮: ""玉米泛转录组的构建及玉米开花抑制因子ZmCOL3的功能解析"", 《中国博士学位论文全文数据库 (农业科技辑)》 * |
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