CN112654709A - 褐藻糖胶的酶促水解 - Google Patents

褐藻糖胶的酶促水解 Download PDF

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Publication number
CN112654709A
CN112654709A CN201980050275.7A CN201980050275A CN112654709A CN 112654709 A CN112654709 A CN 112654709A CN 201980050275 A CN201980050275 A CN 201980050275A CN 112654709 A CN112654709 A CN 112654709A
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CN
China
Prior art keywords
fucoidan
seq
fucosidase
laminaria
hydrolyzed
Prior art date
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Pending
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CN201980050275.7A
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English (en)
Chinese (zh)
Inventor
A.博拉斯顿
C.J.维克斯
O.E.萨拉马-阿尔伯
K.T.阿贝
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ARC Medical Devices Inc
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ARC Medical Devices Inc
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Publication of CN112654709A publication Critical patent/CN112654709A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
CN201980050275.7A 2018-07-31 2019-07-24 褐藻糖胶的酶促水解 Pending CN112654709A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862712312P 2018-07-31 2018-07-31
US62/712,312 2018-07-31
PCT/CA2019/051025 WO2020024043A1 (en) 2018-07-31 2019-07-24 Enzymatic hydrolysis of fucans

Publications (1)

Publication Number Publication Date
CN112654709A true CN112654709A (zh) 2021-04-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980050275.7A Pending CN112654709A (zh) 2018-07-31 2019-07-24 褐藻糖胶的酶促水解

Country Status (11)

Country Link
EP (1) EP3830272A1 (ko)
JP (1) JP2021531791A (ko)
KR (1) KR20210038539A (ko)
CN (1) CN112654709A (ko)
AU (1) AU2019314769A1 (ko)
BR (1) BR112021000629A2 (ko)
CA (1) CA3106453A1 (ko)
IL (1) IL280167A (ko)
MX (1) MX2021000859A (ko)
SG (1) SG11202100386XA (ko)
WO (1) WO2020024043A1 (ko)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0759564A (ja) * 1993-08-23 1995-03-07 Tousa Kogaku Kenkyusho:Kk フコイダン分解酵素及びその製造方法
WO2000017215A1 (fr) * 1998-09-21 2000-03-30 Laboratoires Goemar S.A. Endofucanases et procede les mettant en oeuvre pour la preparation des fuco-oligosaccharides a partir des fucanes, bacterie productrice des endofucanases et applications des fuco-oligosaccharides a la protection de plantes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0759564A (ja) * 1993-08-23 1995-03-07 Tousa Kogaku Kenkyusho:Kk フコイダン分解酵素及びその製造方法
WO2000017215A1 (fr) * 1998-09-21 2000-03-30 Laboratoires Goemar S.A. Endofucanases et procede les mettant en oeuvre pour la preparation des fuco-oligosaccharides a partir des fucanes, bacterie productrice des endofucanases et applications des fuco-oligosaccharides a la protection de plantes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BORASTON, A.B., ET AL.: "Genbank accession number:AYF59292.1", GENBANK, pages 1 *
BORASTON, A.R., ET AL: "Genbank accession number:AYF59291.1", GENBANK, pages 1 *
CHELSEA VICKERS, ET AL.: "Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to -L-fucosidases from GH29", J. BIOL. CHEM., pages 18296 *
COLIN S, ET AL.: "Cloning and biochemical characterization of the fucanase FcnA: definition of a novel glycoside hydrolase family specific for sulfated fucans", GLYCOBIOLOGY, vol. 16, no. 11, pages 1021 - 1032, XP055364380, DOI: 10.1093/glycob/cwl029 *

Also Published As

Publication number Publication date
CA3106453A1 (en) 2020-02-06
KR20210038539A (ko) 2021-04-07
EP3830272A1 (en) 2021-06-09
BR112021000629A2 (pt) 2021-04-06
MX2021000859A (es) 2021-11-04
AU2019314769A1 (en) 2021-02-04
IL280167A (en) 2021-03-01
WO2020024043A1 (en) 2020-02-06
JP2021531791A (ja) 2021-11-25
SG11202100386XA (en) 2021-03-30

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Application publication date: 20210413