CN112624874B - Production method of bio-organic fertilizer based on medicine residues in shishu injection - Google Patents
Production method of bio-organic fertilizer based on medicine residues in shishu injection Download PDFInfo
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Abstract
The invention provides a method for producing a bio-organic fertilizer based on shishu injection herb residues, which comprises the steps of taking the shishu injection herb residues as raw materials, adding bean residues and/or rice husk carbon with the mass of 50-80 wt%, mixing to obtain composite residues, mixing and culturing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, lactobacillus buchneri and candida utilis to prepare composite bacteria liquid, adding the composite bacteria liquid into the composite residues, mixing and stirring uniformly, and carrying out aerobic fermentation until the total effective viable count is not less than 1.0 multiplied by 10 9 cfu/g, and preparing the bio-organic fertilizer. The invention realizes the resource utilization of the medicine residue in the shishu injection, avoids the resource waste and the environmental pollution, the content of organic matters (dry basis) of the prepared bio-organic fertilizer is more than or equal to 55 percent, and the total effective viable count is more than or equal to 1.0 multiplied by 10 9 cfu/g, water content less than or equal to 30 percent and pH value of 6.6 to 7.8.
Description
Technical Field
The invention belongs to the technical field of fertilizers, relates to a production method of a biological organic fertilizer, and particularly relates to a method for producing a biological organic fertilizer by using traditional Chinese medicine residues.
Background
The traditional Chinese medicine industry is the traditional dominant industry in China. In recent years, with the development of the traditional Chinese medicine industry and the enhancement of health consciousness of people, the application of Chinese herbal medicines and Chinese patent medicines is increasingly wide, and simultaneously, a large amount of waste of Chinese medicine residues is also inevitably generated. According to statistics, the Chinese medicine residue discharged every year is up to 1000 ten thousand tons.
The traditional Chinese medicine residue is residue left after traditional Chinese medicines are extracted by a certain solvent or other modes, and mainly comes from the production of Chinese patent medicine raw material medicines, the processing and processing of traditional Chinese medicine decoction pieces, the production process of light chemical products containing traditional Chinese medicine components and the like, wherein the traditional Chinese medicine residue produced in the production process of the Chinese patent medicine accounts for about 70 percent of the total amount.
The traditional Chinese medicine residues are generally wet materials, have water content of more than 70 percent, contain a large amount of crude fibers, starch, crude fat, crude protein, amino acid, trace elements and other nutritional ingredients, are extremely easy to decay, and can cause serious environmental problems if the traditional Chinese medicine residues are not treated in time. More seriously, if these residues are recovered by lawless persons for the manufacture of counterfeit drugs, etc., immeasurable losses will be brought to the drug manufacturers and consumers.
The traditional Chinese medicine residue treatment method comprises landfill, incineration, stacking and the like. Because the Chinese medicine dregs have large water content and low heat value, a large amount of percolate is generated in both landfill and stacking treatment, and the percolate is easy to mildew and rot and pollutes soil. And the incineration treatment needs to add auxiliary fuel, which greatly increases the treatment cost of the traditional Chinese medicine residue.
The Chinese medicine dregs are recycled, so that the environment pollution can be prevented, and the additional value can be created. In recent years, researchers have developed resource utilization methods of a lot of traditional Chinese medicine residues, mainly focusing on extracting other effective components, composting, cultivating edible fungi, acting as animal feed, acting as seedling raising substrate, producing biogas by fermentation and the like.
The biological organic fertilizer is prepared by adding functional microbial agents into organic materials which are mainly prepared from animal and plant residues (such as livestock and poultry manure, crop straws and the like) and subjected to harmless treatment and decomposition on the basis of composting and performing secondary fermentation.
Compared with other fertilizers, the biological organic fertilizer has complete nutrient elements, is rich in a large amount of various functional microbial agents, can assist in releasing potential nutrients in soil and promote the proliferation of beneficial microorganisms at the rhizosphere of crops, and has the advantages of high bioavailability, long action period, low cost, no harm to soil and environment and the like. The biological organic fertilizer can effectively improve soil, improve soil fertility, regulate and control soil and rhizosphere microecological balance, and improve crop disease and insect resistance and crop product quality.
The Chinese medicine dregs are mostly roots, stems and leaves of plants, and contain rich organic matters, saccharides, crude protein, alkaloid, a small amount of vitamins, a large amount of cellulose, nitrogen, phosphorus, potassium and the like required by soil. The biological organic fertilizer prepared by using the traditional Chinese medicine residues has the characteristics of light weight, good air permeability and the like, and can be widely used for planting vegetables, fruits, crops, traditional Chinese medicinal materials and other crops.
CN 102826918A discloses a method for producing an organic fertilizer by using traditional Chinese medicine residues, which takes traditional Chinese medicine residues, corncobs and clay as raw materials, and wood chips as a zymocyte liquid carrier, and respectively carries out fermentation by bacillus subtilis and bacillus stearothermophilus and secondary fermentation by bacillus laterosporus, bacillus megaterium and trichoderma koningii to prepare the organic fertilizer. The organic fertilizer has higher organic matter content, and has better effects on improving soil property, improving soil fertility, promoting plant growth, avoiding the problems of soil hardening, soil degradation and the like caused by industrial fertilizers.
CN 102731195A relates to an organic-inorganic compound fertilizer produced by Chinese medicine residue fermentation and a method thereof, which is to dry and wet the Chinese medicine residue, properly add crude fiber degrading bacteria and the like for mixed fermentation to obtain a semi-finished product of the Chinese medicine residue fermentation organic fertilizer, add chemical fertilizers according to the content of required nitrogen, phosphorus and potassium components, mix and granulate to prepare the fertilizer. The product contains organic matter, inorganic components of nitrogen, phosphorus and potassium, and has the advantages of comprehensive nutrient components, high efficiency, no public nuisance, etc.
The compound kuh-seng injection (shishu injection) is a pure traditional Chinese medicine anticancer preparation developed by cooperative attack of Shanxi Zhendong pharmaceutical products limited company and Chinese medicine institute of Chinese medicine academy of sciences, china center for science and technology communication of Chinese medicine administration, shanxi province tumor hospitals and other units. The Yanshu injection has the effects of relieving pain, stopping bleeding, resisting tumor, regulating immunity, protecting liver and the like, and is clinically used for treating cancer pain, preventing and treating radioactive mucosal injury, lung cancer, breast cancer, gastric cancer, liver cancer, colon cancer, rectal cancer, esophageal cancer, cervical cancer, late-stage cancer and the like.
The Yanshu injection is prepared by selecting two traditional Chinese medicinal materials of sophora flavescens and rhizoma smilacis glabrae, and refining the traditional Chinese medicinal materials through the production processes of acid water percolation, multiple alcohol precipitation and the like, and a large amount of traditional Chinese medicine residues and alcohol precipitation byproducts are generated in the production process. The annual output of the Yanshu injection is about 400 tons, and the amount of the generated traditional Chinese medicine residues is at least 800 tons.
However, based on the characteristics of the production process, the medicine residue in the shishu injection has high acidity, cannot be directly discharged or used, and is not suitable to be used as a raw material for producing the bio-organic fertilizer.
If the traditional Chinese medicine residues are directly stacked or buried, the pH value of surrounding soil is reduced, acid soil is formed, the soil biological activity is influenced, the soil nutrient form is changed, the nutrient effectiveness is reduced, the soil hardening is aggravated, the plant growth vigor is weakened, the disease resistance is reduced, the activation of toxic heavy metal elements in the soil can be caused, the crop growth is influenced, and the health of human bodies or animals is harmed through a food chain. If the traditional Chinese medicine residues are burnt, other harmful substances are discharged into the atmosphere, and secondary pollution is caused to the environment.
Disclosure of Invention
The invention aims to provide a production method of a bio-organic fertilizer based on rock-relaxing injection traditional Chinese medicine residues, so that the rock-relaxing injection traditional Chinese medicine residues can be recycled, and resource waste and environmental pollution are avoided.
The production method of the bio-organic fertilizer based on the medicine residue in the shishu injection comprises the following steps:
1) Taking the traditional Chinese medicine residue of the shishu injection as a raw material, adding bean dregs and/or rice husk carbon with the mass of 50-80 wt%, and mixing to obtain composite residue;
2) Mixing and culturing bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, lactobacillus buchneri and candida utilis to prepare a compound bacterial liquid;
3) Adding compound bacterial liquid into the compound slag, mixing and stirring uniformly, and carrying out aerobic fermentation until the total effective viable count is not less than 1.0 multiplied by 10 9 cfu/g, and preparing the bio-organic fertilizer.
Wherein, the mixture of the bean dregs and the rice husk charcoal with the mass ratio of (1-3) to 1 is preferably added into the medicine dregs of the shishu injection.
The composite bacterial liquid of the invention is prepared from the bacterial liquid with equivalent effective viable countThe bacillus subtilis liquid, the bacillus megatherium liquid, the bacillus mucilaginosus liquid, the lactobacillus buchneri liquid and the candida utilis liquid are mixed and cultured according to the volume ratio of 1 to (0.8-2.0) to (0.6-1.4) to (0.8-1.5) to (2.0-3.0), and the total effective viable count is not less than 1.0 multiplied by 10 5 cfu/ml of composite bacterial liquid.
Furthermore, the invention adds 0.5-1.4L of the total effective viable count not less than 1.0 multiplied by 10 into each kilogram of the composite slag 5 And (3) mixing and stirring the cfu/ml composite bacterial liquid uniformly, and performing aerobic pile fermentation at the temperature of 30-35 ℃ to prepare the bio-organic fertilizer.
Further, during the aerobic fermentation, the pile is preferably turned once every 5 to 7 days.
Generally, after aerobic fermentation is carried out for 14-21 days, the content of effective viable count in the obtained bio-organic fertilizer can meet the index requirement.
The content of organic matters (dry basis) in the biological organic fertilizer prepared by the method is more than or equal to 55 percent, and the total effective viable count is more than or equal to 1.0 multiplied by 10 9 cfu/g, the water content of the bio-organic fertilizer is less than or equal to 30 percent, and the pH value is 6.6-7.8.
The screened beneficial microorganism compound bacterial liquid is adopted to ferment the traditional Chinese medicine residue compound slag of the shishu injection to prepare the bio-organic fertilizer, and a large amount of nutrient components such as amino acid, crude protein, crude fiber and crude fat, a plurality of inorganic elements such as nitrogen, phosphorus, potassium, calcium, manganese, magnesium and iron, and a small amount of vitamins remained in the traditional Chinese medicine residue are comprehensively utilized and treated, so that the bio-organic fertilizer is converted into a high-quality bio-organic fertilizer which is safe and pollution-free and does not contain pathogenic bacteria and toxic substances.
Furthermore, the probiotics in the compound bacterial liquid of the invention takes the mixture of the traditional Chinese medicine residue, the bean dregs and the rice husk charcoal as a substrate to carry out the metabolic activity per se, decompose lignin, cellulose and other components, generate a large amount of beneficial metabolites, improve the acidity characteristic of the traditional Chinese medicine residue per se, maintain the pH value of the bio-organic fertilizer at neutral basically and avoid the harm brought by soil acidification.
The phosphorus-dissolving and potassium-dissolving bacteria in the composite bacterial liquid can decompose the insoluble phosphorus and potassium in the soil into quick-acting phosphorus and potassium compounds which can be absorbed and utilized by crops, so that the supply of nutrient elements in the growth environment of the crops is increased, and the soil fertility is improved; the lactobacillus can reduce plant diseases and insect pests, enhance the disease resistance of crops and prevent pathogenic microorganisms from damaging the crops.
The biological organic fertilizer can promote the growth of crops, particularly the development of root systems, and the developed root systems improve the nutrient absorption capacity of the crops and accelerate the growth and development speed of the crops.
The use of the biological organic fertilizer is also beneficial to reducing the using amount of chemical fertilizers and pesticides and improving the ecological environment, thereby reducing the environmental pollution, promoting the sustainable development of agriculture in China and providing reference basis for other pharmaceutical enterprises in the aspect of recycling the traditional Chinese medicine residues.
Drawings
FIG. 1 shows germination conditions of seeds cultured for 72h in experimental group and control group of bio-organic fertilizer.
FIG. 2 shows the germination conditions of the seeds cultured in the experimental group of shishu injection and the compound bacteria liquid for 96h with the control group.
Detailed Description
The following further describes the embodiments of the present invention with reference to the drawings and examples. The following examples are only for more clearly illustrating the technical solutions of the present invention so as to enable those skilled in the art to better understand and utilize the present invention, and do not limit the scope of the present invention.
The names and the abbreviations of the production processes, instruments and equipment related in the embodiments of the present invention are conventional names in the art, and are clearly and clearly understood in the related fields of use, and those skilled in the art can understand the conventional process steps and apply the corresponding equipment according to the names, and implement the processes according to the conventional conditions or conditions suggested by the manufacturers.
The other raw materials or reagents used in the examples of the present invention are not particularly limited in terms of the sources, except for the sources of the materials and strains listed below, and are conventional products commercially available.
The medicine residue in the shishu injection is produced in an extraction workshop of Shanxi Zhendong pharmaceutical Co.
Bean dregs, produced by Tianxing rice corporation, wuhe county, anhui province.
Rice husk charcoal, produced by subspecies industries, inc., dong Liaoning.
Bacillus subtilis (A), (B) and (C)Bacillus subtilis) The strain number ACCC 19743 is from China agricultural microbial strain preservation management center.
Giant spore Bacillus (A), (B)Bacillus megaterium) The strain number ACCC 02991 is from the China agricultural microbial strain preservation management center.
Bacillus mucilaginosus (B) ((B))Paenibacillus mucilaginosus) The strain number ACCC 19749 is from China agricultural microbial strain preservation management center.
Bacillus mucilaginosus (B.), (B.mucilaginosus)Paenibacillus mucilaginosus) The strain number ACCC 10013 is from China agricultural microbial strain preservation management center.
Lactobacillus buchneri (B.buchneri) (B.buchneri)Lactobacillus buchneri) The strain number is CGMCC 1.15607, and is from China general microbiological culture Collection center.
Candida utilis (A), (B)Candida untilis) The strain number CICC 1314 is from China center for culture Collection of industrial microorganisms.
Example 1.
The shishu injection is prepared by two medicinal materials of kuh-seng and rhizoma smilacis glabrae which are percolated by acid water and precipitated by alcohol for a plurality of times, and a large amount of dregs and by-products of precipitation by alcohol are generated in the production process.
Adding 1750kg of radix Sophorae Flavescentis decoction pieces and 750kg of rhizoma Smilacis Glabrae decoction pieces into a percolation tank, adding 10000kg of 1% acetic acid solution, soaking at 20 + -2 deg.C for 30 hr, and percolating with 5000kg of 1% acetic acid solution. After 24 hours of percolation, all the liquid medicine in the tank is collected.
Pouring the residue into a decocting tank, adding 2.5 times of purified water, decocting at 95 deg.C for 60 min, decocting once again under the same conditions, sieving the two decoctions with 200 mesh sieve, and collecting to a liquid collecting tank.
Drying the discharged dregs in the sun, placing in a swing type pulverizer, and coarsely pulverizing until the diameter of the dregs is about 1cm to obtain dry medicine dregs of the shishu injection.
Measuring carbon and nitrogen elements in the Chinese medicine residue by using an element analyzer; measuring elements such as P, ca, mg, fe, K, etc. in the Chinese medicine residue by using a chemical analysis method; and (3) determining the moisture of the traditional Chinese medicine residues by using a drying method, and determining the ash content of the traditional Chinese medicine residues by using a burning weight method. The results are shown in Table 1.
Example 2.
Respectively taking freeze-dried powder of bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, lactobacillus buchneri and candida utilis, and carrying out activation culture according to the requirements of specifications of various strains strictly to obtain the effective viable count of not less than 1.0 multiplied by 10 5 cfu/ml of bacterial liquid.
A complex medium was prepared from 2g of ammonium sulfate, 3g of glucose, 7g of potato starch, 0.3g of dipotassium hydrogen phosphate, 0.2g of sodium chloride, 0.8g of magnesium sulfate, 0.03g of manganese sulfate, 0.03g of ferrous sulfate, 3g of yeast powder, 1L of distilled water, and pH 7.0.
Transferring Bacillus subtilis solution 5.0ml, bacillus megaterium solution 4.0ml, bacillus mucilaginosus solution 3.0ml, bacillus mucilaginosus solution 4.0ml, lactobacillus buchneri solution 10.0ml, candida utilis solution 10.0ml, inoculating into 1L composite culture medium, and shake culturing at 30 + -2 deg.C for 12 hr to obtain total effective viable count of 1.8 × 10 5 cfu/ml of composite bacterial liquid.
Example 3.
600g of the medicine residue in the shishu injection prepared in the embodiment 1 is weighed and fully and uniformly mixed with 250g of bean dregs and 150g of rice husk charcoal to obtain 1000g of composite residue.
700ml of the compound bacterial liquid prepared in the embodiment 2 is added into the compound slag, mixed and stirred evenly, aerobic pile fermentation is carried out at the temperature of 30-35 ℃, and the pile is turned for 1 time every 5 days in the period. And fermenting for 21 days to obtain a bio-organic fertilizer product.
Through detection, the total effective viable count of the bio-organic fertilizer prepared by the embodiment is 6.83 multiplied by 10 9 cfu/g, the content of organic matters (dry basis) 78.32%, the content of total nutrients (nitrogen, phosphorus and potassium) 3.34%, the water content 26%, the pH value 6.87, and other heavy metal elements and biochemical indexes all meet the index requirements specified in NY 884-2012 standard.
Example 4.
500g of the medicine residue in the shishu injection prepared in the embodiment 1 is weighed and fully and uniformly mixed with 300g of bean dregs and 200g of rice husk charcoal to obtain 1000g of composite residue.
Transferring 1.0ml of Bacillus subtilis solution, 2.0ml of Bacillus megaterium solution, 1.4ml of Bacillus mucilaginosus solution, 1.5ml of Bacillus mucilaginosus solution, 3.0ml of Lactobacillus buchneri solution and 3.0ml of Candida utilis solution in example 2, inoculating into 1400ml of the composite culture medium in example 2, and performing shake culture at 30 +/-2 ℃ for 12h to obtain the total effective viable count of 3.71 multiplied by 10 5 cfu/ml of composite bacterial liquid.
Adding the compound bacterial liquid into the compound slag, mixing and stirring uniformly, carrying out aerobic pile fermentation at 30-35 ℃, turning the pile for 1 time every 7 days during the period, and fermenting for 14 days to obtain the bio-organic fertilizer product.
Through detection, the total effective viable count of the bio-organic fertilizer prepared by the embodiment is 5.17 multiplied by 10 10 cfu/g, the content of organic matters (dry basis) 84.57%, the content of total nutrients (nitrogen, phosphorus and potassium) 4.09%, the water content is 29%, the pH value is 7.16, and other heavy metal elements and biochemical indexes all meet the index requirements specified in NY 884-2012 standard.
Example 5.
1200g of the medicine residue of the shishu injection prepared in the example 1 is weighed and fully and uniformly mixed with 200g of bean dregs and 200g of rice husk charcoal to obtain 1600g of composite residue.
Transferring 1.2ml of Bacillus subtilis solution, 1.7ml of Bacillus megaterium solution, 1.2ml of Bacillus mucilaginosus solution, 1.4ml of Bacillus mucilaginosus solution, 3.0ml of Lactobacillus buchneri solution and 2.8ml of Candida utilis solution in example 2, inoculating into 1500ml of the composite culture medium in example 2, and performing shake culture at 30 +/-2 ℃ for 12h to obtain the total effective viable count of 5.04 multiplied by 10 5 cfu/ml of composite bacterial liquid.
And adding the compound bacterial liquid into the compound slag, mixing and stirring uniformly, carrying out aerobic pile fermentation at 30-35 ℃, turning the piles for 1 time every 7 days during the period, and fermenting for 19 days to obtain the bio-organic fertilizer product.
Through detection, the total effective viable count of the bio-organic fertilizer prepared by the embodiment is 8.72 multiplied by 10 9 cfu/g, the content of organic matters (dry basis) 75.79%, the content of total nutrients (nitrogen, phosphorus and potassium) 3.27%, the water content 23%, the pH value 7.53, and other heavy metal elements and biochemical indexes all meet the index requirements specified in the NY 884-2012 standard.
Example 6.
The germination index of the seeds comprehensively reflects the phytotoxicity of the compost and is considered as the most sensitive and reliable compost maturity evaluation index. Generally, the germination index is greater than 50% and the compost is considered to be substantially non-toxic to the seeds.
The bio-organic fertilizers prepared in examples 3 and 4 were used as observation targets, and the influence of the bio-organic fertilizers on the germination index of seeds was detected to determine the degree of decomposition of the fertilizers.
Weighing 20g of each of the bio-organic fertilizers in the embodiments 3 and 4, adding the bio-organic fertilizers into water according to the ratio of 1: 10 (m/V), mixing and oscillating the bio-organic fertilizers at the temperature of 30 ℃ for 2 hours at 150rpm, centrifuging the bio-organic fertilizers, and filtering supernate with filter paper to obtain bio-organic fertilizer leaching solutions which are respectively used as samples of the experiment group 1 and the experiment group 2.
Putting a piece of filter paper in a clean and sterile 9cm culture dish, uniformly placing 25 pakchoi seeds on the filter paper, sucking 5ml of each of the samples of the experiment group 1 and the experiment group 2 in the culture dish, culturing for 72 hours in a 25 ℃ incubator, calculating the germination rate, measuring the length of a radicle, and calculating the germination index GI of the seeds.
Each sample was replicated 3 times and blanked with distilled water.
GI calculation formula: GI = (bio-organic fertilizer extract seed germination rate × radicle length)/(distilled water seed germination rate × radicle length) × 100.
Although the plant growth analysis can intuitively reflect the influence of the bio-organic fertilizer on the growth of crops, the test time is long and the labor amount is large. The seed germination test is a direct, rapid and reliable biological test method for testing the toxicity of plants, is influenced by various aspects of performances of the bio-organic fertilizer, and is a comprehensive parameter.
In the leaching solution of the non-decomposed compost, the growth of the plant seeds is inhibited to some extent, while in the leaching solution of the decomposed compost, the growth is promoted. The measurement data of the germination indexes show that the germination indexes of the biological organic fertilizer are all larger than 50%, the biological organic fertilizer is basically nontoxic to plant radices, and meanwhile, the length of the radicle of an experimental group is obviously longer than that of a control group, so that the biological organic fertilizer can promote the growth of the radicle of the seeds.
Example 7.
Weighing 20g of the drug residue in the shishu injection in example 1, adding the drug residue into water according to the ratio of water to water of 1: 10 (m/V), mixing and oscillating the drug residue at the temperature of 30 ℃ and the rpm of 150 for 2h, centrifuging the drug residue, and filtering the supernatant with filter paper to obtain the drug residue leaching liquor in the shishu injection, wherein the drug residue leaching liquor is used as a sample of an experimental group 1.
20ml of the composite bacterial liquid of example 2 was transferred and added to water at a ratio of 1: 10 (V/V) as a sample of experiment group 2.
Putting a piece of filter paper in a clean and sterile 9cm culture dish, uniformly placing 30 pakchoi seeds on the filter paper, sucking 5ml of each sample of the experiment group 1 and the experiment group 2 in the culture dish, culturing for 96h in a constant temperature box at 25 ℃, calculating the germination rate, measuring the length of a radicle, and calculating the germination index GI of the seeds.
And (3) repeating each sample, taking distilled water as a blank control, detecting the seed germination indexes of the medicine residue in the shishu injection and the compound bacteria liquid, and judging the influence of the germination indexes on the seed vitality.
Through comparing the germination index measurement data of the traditional Chinese medicine residue in the shishu injection and the composite bacterial liquid, the germination of seeds is inhibited by the traditional Chinese medicine residue in the shishu injection (the germination index is less than 50%), and the length of radicle is obviously shorter than that of a control group, so that the traditional Chinese medicine residue in the shishu injection is proved to be unsuitable to be directly used as a bio-organic fertilizer matrix due to the acidic property of the traditional Chinese medicine residue in the shishu injection.
The germination index of the compound bacterial liquid is more than 50%, and the radicle length is more than that of the control group, which shows that the compound bacterial liquid has a promotion effect on the growth of seeds.
However, in the comprehensive comparison, the culture time of the present example is 96h, which is greater than the culture time of 72h of example 6, so the radicle length of the control group in the present example is significantly greater than that of example 6, but both the radicle length and germination index of the compound bacterial liquid are inferior to those of example 6, which proves that the effect is not as significant as that of the bio-organic fertilizer.
The above embodiments of the present invention are not intended to be exhaustive or to limit the invention to the precise form disclosed. Various changes, modifications, substitutions and alterations to these embodiments will be apparent to those skilled in the art without departing from the principles and spirit of this invention.
Claims (4)
1. A production method of a bio-organic fertilizer based on medicine residue in a shishu injection is characterized by comprising the following steps:
1) Taking the traditional Chinese medicine dregs of the shishu injection as raw materials, adding a mixture of bean dregs and rice husk carbon with the mass of 50-80 wt%, and mixing to obtain composite dregs, wherein the mass ratio of the bean dregs to the rice husk carbon in the mixture is (1-3) to 1;
2) The bacillus subtilis with the strain number of ACCC 19743, the bacillus megaterium with the strain number of ACCC 02991, the bacillus mucilaginosus with the strain number of ACCC 19749, the bacillus mucilaginosus with the strain number of ACCC 10013, the lactobacillus buchneri with the strain number of CGMCC 1.15607 and the candida utilis with the strain number of CICC 1314 are mixed and cultured to prepare a composite bacterial liquid, wherein the composite bacterial liquid is prepared by mixing and culturing bacillus subtilis liquid, bacillus megaterium liquid, bacillus mucilaginosus liquid, lactobacillus buchneri liquid and candida utilis bacterial liquid with equal effective viable count according to the volume ratio of 1 to (0.8-2.0) to (0.6-1.4) to (0.8-1.5) to(2.0-3.0) to (2.0-3.0), and the total effective viable count is not less than 1.0 multiplied by 10 5 cfu/ml of composite bacterial liquid;
3) Adding compound bacterial liquid into the compound slag, mixing and stirring uniformly, and carrying out aerobic fermentation until the total effective viable count is not less than 1.0 multiplied by 10 9 cfu/g, and preparing the bio-organic fertilizer.
2. The method for producing the biological organic fertilizer as claimed in claim 1, wherein 0.5 to 1.4L of the total effective viable bacteria is added into each kilogram of the composite slag and is not less than 1.0 x 10 5 cfu/ml of the compound bacterial liquid, mixing and stirring evenly, and carrying out aerobic fermentation at the temperature of 30-35 ℃.
3. The method for producing the bio-organic fertilizer as claimed in claim 1, wherein the pile is turned over every 5 to 7 days during the aerobic fermentation.
4. The bio-organic fertilizer prepared by the production method of the bio-organic fertilizer of claim 1, wherein the content of organic matters in the bio-organic fertilizer on a dry basis is more than or equal to 55 percent, and the total effective viable count is more than or equal to 1.0 x 10 9 cfu/g, water content less than or equal to 30 percent and pH value of 6.6 to 7.8.
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| CN102826918A (en) * | 2012-09-25 | 2012-12-19 | 南开大学 | Method for producing organic fertilizer from traditional Chinese medicine dregs |
| CN103396223B (en) * | 2013-07-29 | 2015-08-12 | 成都市四友化学工业有限责任公司 | A kind of take Chinese medicine slag as biological organic fertilizer and the production technique thereof of raw material |
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