CN112546114A - Gynecological bacteriostatic nursing lotion and preparation method and use method thereof - Google Patents
Gynecological bacteriostatic nursing lotion and preparation method and use method thereof Download PDFInfo
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Abstract
The invention discloses a gynecological bacteriostatic nursing lotion, a preparation method and a use method thereof, wherein the gynecological bacteriostatic nursing lotion comprises the following raw material components in parts by weight: 20-30 parts of phellodendron, 20-30 parts of Baeckea frutescens, 100-120 parts of fructus cnidii, 100-120 parts of corydalis bungeana, 20-30 parts of sophora alopecuroides, 35-45 parts of Baeckea frutescens extract, 20-30 parts of sophora alopecuroides extract, 10-20 parts of Baeckea volatile oil, 2-3 parts of borneol, 0-20 parts of tween-8010, 8-10 parts of sodium benzoate and 1-2 parts of berberine hydrochloride.
Description
Technical Field
The invention relates to the technical field of female washing liquid, in particular to gynecological bacteriostatic nursing washing liquid and a preparation method and a using method thereof.
Background
The gynecological bacteriostatic nursing lotion has the functions of clearing heat and removing toxicity, purging fire and drying dampness, and killing parasites and relieving itching. Can be used for treating pruritus vulvae and leukorrhagia caused by downward flow of damp-heat, and has the following symptoms: vulvar vaginal burning and itching, vaginal secretion increase, yellow thick and smelly, etc., and trichomonas vaginitis, mycotic vaginitis and nonspecific vaginitis with the above syndromes.
The invention improves the preparation method on the basis of the prior art, so that the effectiveness of the obtained product is more obvious, and the gynecological bacteriostatic nursing lotion with more definite and effective treatment effect is obtained.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides an improved gynecological bacteriostatic care lotion, a preparation method and a use method thereof, so that the effectiveness of the obtained product is more obvious, and the bacteriostatic effect is greatly improved.
The technical scheme provided by the invention is as follows:
the gynecological bacteriostatic nursing lotion comprises the following raw material components in parts by weight: 20-30 parts of phellodendron, 20-30 parts of Baeckea frutescens, 100-120 parts of fructus cnidii, 100-120 parts of corydalis bungeana, 20-30 parts of sophora alopecuroides, 35-45 parts of Baeckea frutescens extract, 20-30 parts of sophora alopecuroides extract, 10-20 parts of Baeckea volatile oil, 2-3 parts of borneol, 0-20 parts of tween-8010, 8-10 parts of sodium benzoate and 1-2 parts of berberine hydrochloride.
Preferably, the feed comprises the following raw material components in parts by weight: 20 parts of phellodendron, 20 parts of roughhaired holly bark, 100 parts of common cnidium fruit, 100 parts of corydalis bungeana, 20 parts of sophora alopecuroides, 35 parts of roughhaired holly bark extract, 20 parts of sophora alopecuroides extract, 20 parts of roughhaired holly bark volatile oil, 2.4 parts of borneol, 20 parts of tween-8020, 10 parts of sodium benzoate and 2 parts of berberine hydrochloride.
Preferably, the composition also comprises 0.3-0.5 part of chlorhexidine acetate, 0.3-0.5 part of benzalkonium chloride or 0.3-0.5 part of PCMX in parts by weight.
The preparation method of the gynecological bacteriostatic nursing lotion specifically comprises the following operation steps:
s1, extraction: adding 8-10 times of water into phellodendron amurense, baeckea frutescens, fructus cnidii, bunge corydalis herb and sophora alopecuroides, decocting for 0.5h, and then refluxing for 3-4 h;
s2, concentrated preparation: boiling the baeckea frutescens extract, the sophora alopecuroide extract, berberine hydrochloride and 1/5-1/4 volume of extraction concentrate for 1-2 hours, mixing the rest extraction concentrate, putting the mixture into an alcohol precipitation tank for low-temperature refrigeration for 24 hours, filtering the refrigerated solution, heating to boil for 1-2 hours, and cooling and refrigerating to obtain a secondary refrigerated solution;
s3, diluting and preparing: dissolving Borneolum in the volatile oil, adding Tween-80, stirring, adding 1/2 volumes of purified water, stirring to form emulsion, diluting with purified water, adding into the cold storage solution, stirring, dissolving sodium benzoate, adding into the mixed solution, stirring to dissolve completely, adding purified water to desired volume, filtering, and packaging.
Preferably, the refrigeration temperature is 6-8 ℃.
Preferably, the filling is of a 150 ml/bottle or 200 ml/bottle size.
The application method of the gynecological bacteriostatic nursing lotion comprises the following steps: the gynecological bacteriostatic nursing lotion is prepared into liquid with the concentration of 10% to wash vulva and vagina.
Preferably, 15-20 ml of the gynecological antibacterial nursing lotion is used each time, once a day, and 7 days are a treatment course.
The invention has the beneficial effects that:
compared with the prior art, the gynecological antibacterial nursing lotion provided by the invention has stronger antibacterial action, has the effects of clearing away heat and toxic materials, purging fire, eliminating dampness, killing parasites and relieving itching, has great effects on symptoms such as burning heat and itching of vagina, increased vaginal secretion, yellow, thick and smelly vagina and the like, and also has the effects on trichomonas vaginitis, mycotic vaginitis and nonspecific vaginitis with the symptoms, and the preparation method is simple and has popularization and application values.
Detailed Description
Hereinafter, embodiments of the present invention will be described in detail. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Example 1
The embodiment provides a gynecological bacteriostatic nursing lotion which comprises the following raw material components in parts by weight: 20 parts of phellodendron, 20 parts of roughhaired holly bark, 100 parts of common cnidium fruit, 100 parts of corydalis bungeana, 20 parts of sophora alopecuroides, 35 parts of roughhaired holly bark extract, 20 parts of sophora alopecuroides extract, 20 parts of roughhaired holly bark volatile oil, 2.4 parts of borneol, tween-8020 parts, 10 parts of sodium benzoate, 2 parts of berberine hydrochloride and 0.5 part of chlorhexidine acetate
The preparation method of the gynecological bacteriostatic nursing lotion specifically comprises the following operation steps:
s1, extraction: adding 8-10 times of water into phellodendron amurense, baeckea frutescens, fructus cnidii, bunge corydalis herb and sophora alopecuroides, decocting for 0.5h, and then refluxing for 3-4 h;
s2, concentrated preparation: boiling the baeckea frutescens extract, the sophora alopecuroide extract, berberine hydrochloride and 1/5-1/4 volume of extraction concentrate for 1-2 hours, mixing the rest extraction concentrate, putting the mixture into an alcohol precipitation tank for low-temperature refrigeration for 24 hours, filtering the refrigerated solution, heating to boil for 1-2 hours, and cooling and refrigerating, wherein the refrigerating temperature is 6-8 ℃ to obtain a secondary refrigerated solution;
s3, diluting and preparing: dissolving Borneolum in the volatile oil, adding Tween-80, stirring, adding 1/2 volumes of purified water, stirring to form emulsion, diluting with purified water, adding into the cold storage solution, stirring, dissolving sodium benzoate, adding into the mixed solution, stirring to dissolve completely, adding purified water to desired volume, filtering, and bottling (150 ml/bottle).
The application method of the gynecological bacteriostatic nursing lotion comprises the following steps: the gynecological bacteriostatic nursing lotion is prepared into liquid with the concentration of 10% to wash vulva and vagina. The gynecological antibacterial nursing lotion is used 15-20 ml each time, once a day, and 7 days are a course of treatment.
Example 2
The embodiment provides a gynecological bacteriostatic nursing lotion which comprises the following raw material components in parts by weight: 20 parts of phellodendron, 20 parts of roughhaired holly bark, 100 parts of common cnidium fruit, 100 parts of corydalis bungeana, 20 parts of sophora alopecuroides, 35 parts of roughhaired holly bark extract, 20 parts of sophora alopecuroides extract, 20 parts of roughhaired holly bark volatile oil, 2.4 parts of borneol, 8020 parts of tween-8020, 10 parts of sodium benzoate, 2 parts of berberine hydrochloride and 0.3 part of benzalkonium chloride.
Example 3
The embodiment provides a gynecological bacteriostatic nursing lotion which comprises the following raw material components in parts by weight: 20 parts of phellodendron, 20 parts of roughhaired holly bark, 100 parts of common cnidium fruit, 100 parts of corydalis bungeana, 20 parts of sophora alopecuroides, 35 parts of roughhaired holly bark extract, 20 parts of sophora alopecuroides extract, 20 parts of roughhaired holly bark volatile oil, 2.4 parts of borneol, 20 parts of tween-8020, 10 parts of sodium benzoate, 2 parts of berberine hydrochloride and 0.4 part of PCMX.
Example 4
The gynecological bacteriostatic care lotion prepared in example 1 was subjected to bacteriostatic performance tests (candida albicans), which are as follows:
(1) equipment
Test strains: candida albicans (ATCC10231), supplied by North Na Biotech, Inc., was cultured for passage 3.
The culture medium is sand type agar culture medium
Equipment of electric heating constant temperature incubator (WPE2948) and biological safety cabinet (WPE2977)
(2) Method of producing a composite material
The inspection basis is as follows: test method for bacteriostatic performance of dissoluble antibacterial (bacteriostatic) product in appendix C4 of GB15979-2002 disposable sanitary article standards
Detecting the environment: temperature: 20.7 ℃, relative humidity: 38 percent.
And (3) bacteriostatic test: the gynecological bacteriostatic care lotion (stock solution) prepared in example 1 acts for 2min, 5min, 10min and 20min, and is repeated for 3 times.
(3) Results
The bacteriostatic effect on candida albicans is shown in table 1:
TABLE 1 bacteriostatic effect table for Candida albicans
Note: the average colony number of the positive control group was 5.2X104CFU/mL(5.1x104CFU/mL ～5.3x104CFU/mL)。
Negative control group: PBS, medium, no bacterial growth.
(4) Conclusion
After 3 repeated tests, the gynecological bacteriostatic care lotion (stock solution) respectively acts for 2min, 5min, 10min and 20min, has bacteriostatic rates of Candida albicans of 60.3%, 82.3%, 98.4% and 99.0%, and has bacteriostatic action, thereby meeting the requirements of GB15979-2002 hygienic Standard for Disposable sanitary articles.
Example 5
The gynecological bacteriostatic care lotion prepared in example 1 was subjected to a bacteriostatic performance test (staphylococcus aureus), which specifically comprises the following steps:
(1) equipment
Test strains: staphylococcus aureus (ATCC6538), supplied by Beiner Biotech, Inc., was cultured for the 3 rd generation.
The culture medium is nutrient agar culture medium
Equipment of electric heating constant temperature incubator (WPE2948) and biological safety cabinet (WPE2977)
(2) Method of producing a composite material
The inspection basis is as follows: test method for bacteriostatic performance of dissoluble antibacterial (bacteriostatic) product in appendix C4 of GB15979-2002 disposable sanitary article standards
Detecting the environment: temperature: 20.7 ℃, relative humidity: 38 percent.
And (3) bacteriostatic test: the gynecological bacteriostatic care lotion (stock solution) prepared in example 1 acts for 2min, 5min, 10min and 20min, and is repeated for 3 times.
(3) Results
The bacteriostatic effect on staphylococcus aureus is shown in table 2:
TABLE 2 bacteriostatic effect table for staphylococcus aureus
Note: the average colony number of the positive control group was 3.6X104CFU/mL(3.5x104CFU/mL ～3.7x104CFU/mL)。
Negative control group: PBS, medium, no bacterial growth.
(4) Conclusion
After 3 repeated tests, the gynecological bacteriostatic care lotion (stock solution) respectively acts for 2min, 5min, 10min and 20min, has bacteriostatic rates of 54.6 percent, 68.5 percent, 74.9 percent and 79.6 percent on staphylococcus aureus, has bacteriostatic action, and meets the requirements of GB15979-2002 hygienic standard for disposable use.
Example 6
The gynecological bacteriostatic care lotion prepared in example 1 was subjected to a bacteriostatic performance test (escherichia coli), which specifically comprises the following steps:
(1) equipment
Test strains: escherichia coli (8099) was cultured for the 3 rd generation, provided by the research and development center of food microbial safety engineering in Guangdong province.
The culture medium is nutrient agar culture medium
Equipment of electric heating constant temperature incubator (WPE2948) and biological safety cabinet (WPE2977)
(2) Method of producing a composite material
The inspection basis is as follows: test method for bacteriostatic performance of dissoluble antibacterial (bacteriostatic) product in appendix C4 of GB15979-2002 disposable sanitary article standards
Detecting the environment: temperature: 25.8 ℃, relative humidity: 58 percent.
And (3) bacteriostatic test: the gynecological bacteriostatic care lotion (stock solution) prepared in example 1 acts for 2min, 5min, 10min and 20min, and is repeated for 3 times.
(3) Results
The bacteriostatic effect on escherichia coli is shown in table 3:
TABLE 3 bacteriostatic effect table for E.coli
Note: the average colony number of the positive control group is 3.4x104CFU/mL(3.3x104CFU/mL ～3.4x104CFU/mL)。
Negative control group: PBS, medium, no bacterial growth.
(4) Conclusion
Through repeated experiments for 3 times, the gynecological bacteriostatic care lotion (stock solution) respectively acts for 2min, 5min, 10min and 20min, has the bacteriostatic rate of escherichia coli of more than or equal to 90 percent, has stronger bacteriostatic action, and meets the requirements of GB15979-2002 hygienic Standard for Disposable sanitary articles.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (8)
1. The gynecological bacteriostatic nursing lotion is characterized in that: the material comprises the following raw materials in parts by weight: 20-30 parts of phellodendron, 20-30 parts of Baeckea frutescens, 100-120 parts of fructus cnidii, 100-120 parts of corydalis bungeana, 20-30 parts of sophora alopecuroides, 35-45 parts of Baeckea frutescens extract, 20-30 parts of sophora alopecuroides extract, 10-20 parts of Baeckea volatile oil, 2-3 parts of borneol, 0-20 parts of tween-8010, 8-10 parts of sodium benzoate and 1-2 parts of berberine hydrochloride.
2. The gynecological bacteriostatic care lotion according to claim 1, which is characterized in that: the material comprises the following raw materials in parts by weight: 20 parts of phellodendron, 20 parts of roughhaired holly bark, 100 parts of common cnidium fruit, 100 parts of corydalis bungeana, 20 parts of sophora alopecuroides, 35 parts of roughhaired holly bark extract, 20 parts of sophora alopecuroides extract, 20 parts of roughhaired holly bark volatile oil, 2.4 parts of borneol, 20 parts of tween-8020, 10 parts of sodium benzoate and 2 parts of berberine hydrochloride.
3. The gynecological bacteriostatic care lotion according to claim 2, which is characterized in that: and also comprises 0.3 to 0.5 portion of chlorhexidine acetate, 0.3 to 0.5 portion of benzalkonium chloride or 0.3 to 0.5 portion of PCMX according to the weight portion.
4. A method for preparing a gynecological bacteriostatic care lotion according to claims 1-3, which is characterized in that: the method specifically comprises the following operation steps:
s1, extraction: adding 8-10 times of water into phellodendron amurense, baeckea frutescens, fructus cnidii, bunge corydalis herb and sophora alopecuroides, decocting for 0.5h, and then refluxing for 3-4 h;
s2, concentrated preparation: boiling the baeckea frutescens extract, the sophora alopecuroide extract, berberine hydrochloride and 1/5-1/4 volume of extraction concentrate for 1-2 hours, mixing the rest extraction concentrate, putting the mixture into an alcohol precipitation tank for low-temperature refrigeration for 24 hours, filtering the refrigerated solution, heating to boil for 1-2 hours, and cooling and refrigerating to obtain a secondary refrigerated solution;
s3, diluting and preparing: dissolving Borneolum in the volatile oil, adding Tween-80, stirring, adding 1/2 volumes of purified water, stirring to form emulsion, diluting with purified water, adding into the cold storage solution, stirring, dissolving sodium benzoate, adding into the mixed solution, stirring to dissolve completely, adding purified water to desired volume, filtering, and packaging.
5. The preparation method of the gynecological bacteriostatic care lotion according to claim 4, which is characterized in that: the refrigeration temperature is 6-8 ℃.
6. The preparation method of the gynecological bacteriostatic care lotion according to claim 4, which is characterized in that: the filling specification is 150 ml/bottle or 200 ml/bottle.
7. A method of using the gynecological bacteriostatic care lotion according to claims 1-3, which is characterized in that: the gynecological bacteriostatic nursing lotion is prepared into liquid with the concentration of 10% to wash vulva and vagina.
8. The use method of the gynecological bacteriostatic care lotion according to claim 7, which is characterized in that: the gynecological antibacterial nursing lotion is used 15-20 ml each time, once a day, and 7 days are a course of treatment.
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Citations (4)
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| CN1850202A (en) * | 2005-04-22 | 2006-10-25 | 姜惠民 | Vagina external-use medicine composition and its preparing method and use |
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| CN1850202A (en) * | 2005-04-22 | 2006-10-25 | 姜惠民 | Vagina external-use medicine composition and its preparing method and use |
| CN1943743A (en) * | 2005-10-08 | 2007-04-11 | 曾雄辉 | A medicine for treatment and prevention of vaginitis |
| CN103655866A (en) * | 2013-12-19 | 2014-03-26 | 科创控股集团有限公司 | Instant tablet taking baeckea frutescens as main component and preparation technology of instant tablet |
| CN106822325A (en) * | 2017-04-07 | 2017-06-13 | 成都标典生物科技开发有限公司 | The new preparation method of compound baeckea frutescen washing lotion |
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Application publication date: 20210326 |