CN105767006A - Application of bacillus cereus BCJB01 to prevention and treatment of grape ulcer diseases - Google Patents
Application of bacillus cereus BCJB01 to prevention and treatment of grape ulcer diseases Download PDFInfo
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- CN105767006A CN105767006A CN201610141894.5A CN201610141894A CN105767006A CN 105767006 A CN105767006 A CN 105767006A CN 201610141894 A CN201610141894 A CN 201610141894A CN 105767006 A CN105767006 A CN 105767006A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses application of bacillus cereus BCJB01 to prevention and treatment of grape ulcer diseases, and discloses an antibiological inoculant suspending agent of the bacillus cereus BCJB01. The prevention and treatment effect of the suspending agent on grape clusters is greater than 76.8%, and the prevention and treatment effect on grape fruits is greater than 90.1%.
Description
Technical field
The present invention relates to a kind of bacillus cereus preparation and application, be specially bacillus cereus BCJB01 application in preventing and treating grape ulcer, belong to field of biological control.
Background technology
Grape ulcer (Grapevinecanker) is a kind of serious grape disease, causes tikka, and branch top is withered, downright bad, branch, stem's ulcer, the symptom such as fruit and seed decay, in the whole world, main viny region all has been reported that, and causes extremely serious economic loss.California, USA is every year because the loss of Peptic Ulcers is more than 2.6 hundred million dollars；Australia south, the fungus of Fructus Vitis viniferae seat chamber Cordycepps and eutypa dieback pathogenic bacteria Eutypellavitis mixed infection reach 60%, and Fructus Vitis viniferae is brought about great losses.Also all having generation in the main plantation province of 20 Fructus Vitis viniferaes of China, disease causes the Fructus Vitis viniferae underproduction 3% to 8%, and the serious area underproduction reaches 10% to 20%, and indivedual area vineyard underproduction reach more than 50%.
The Species of Pathogens causing Peptic Ulcers is a lot, external most researchs are thought, the cause of disease causing grape ulcer is mainly the Botryosphaeria Botryosphaeriaspp. in ascomycetes, up to the present, having been found that 15 kinds of Fructus Vitis viniferae seat chamber Cordycepps can cause grape ulcer, the cause of disease causing grape ulcer of China's report is mainly: Fructus Vitis viniferae seat chamber bacterium Botryosphaeriadothidea, Lasiodiplodia theobromae Lasidiplodiatherbromae, color two born of the same parents belong to Diplodiaseriata, little new shell shuttle spore Neofusicoccumparvum.Wherein B.dothidea and L.theobromae is principal causative population.
At present conventional prevention and prevent and treat the method for this disease and have: farmland management improves, application resistant variety, Agro-chemicals control, but effect is very obvious, and chemical pesticide uses and improper causes a series of environment and health problem.Along with growth in the living standard, people are increasing to pollution-free food, Organic food demand, to the demand also expanding day supporting the green of its production, pollution-free, high-performance bio pesticide.Also relevant research has been carried out both at home and abroad, as Song Yaqin etc. passes through to screen, it is recommended to use Imazalil EC prevents and treats grape ulcer Lasidiplodiatherbromae；Liu Mei etc. are proposed with Fluoxastrobin, Difenoconazole or pythium oligandrum fruit ear for grape ulcer Botryosphaeriarhodina, Botryosphaeriadothidea and dip in medicine or spray and prevent and treat, but only rest on laboratory stage.The domestic patent totally 2 preventing and treating grape ulcer, wherein 1 is Biological control microbial inoculum, and with market demand grave fault, therefore developing the microorganism formulation efficiently preventing and treating grape ulcer becomes market in urgent need.
Summary of the invention
The purpose of the present invention is aiming at the defect of above-mentioned existence and provides a kind of bacillus cereus BCJB01 application in preventing and treating grape ulcer.This bacillus cereus is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, postcode: 100101, its deposit number is: CGMCCNO.10477, the Latin title of strain is Bacilluscereus, the microorganism (strain) of ginseng evidence: BCJB01, preservation date is on January 30th, 2015.The present invention discloses the biocontrol agent suspending agent of a kind of bacillus cereus BCJB01, this suspending agent to the prevention effect of grape fruit ear more than 76.8%, to the prevention effect of grape fruit more than 90.1%.
The technical scheme is that, bacillus cereus BCJB01 application in preventing and treating grape ulcer.
Fructus Vitis viniferae seat chamber bacterium and Lasiodiplodia theobromae are had inhibitory action by bacillus cereus BCJB01.
Bacillus cereus BCJB01 fermentation liquid is prepared into biocontrol agent and carries out the preventing and treating of grape ulcer.
Described biocontrol agent is suspending agent, by bacillus cereus BCJB01 fermentation liquid, N-dodecyl-Beta-alanine sodium, carboxymethyl cellulose, sulphuric acid benzene sulfonic acid sodium salt, 2, dipicolimic acid 2 calcium and Peptidoglycan composition.
Described suspending agent consists of the following composition:
In bacillus cereus BCJB01 fermentation liquid, spore content is for being not less than 55 × 108cfu/mL。
In suspending agent, effective active component content is not less than 50 × 108cfu/mL。
In suspending agent, each component particle diameter is not more than 5 μm.
The invention have the benefit that in the present invention, B.dothidea and L.theobromae is had good inhibiting effect, antibacterial bandwidth respectively 7.5mm and 9.5mm by bacillus cereus BCJB01 on PDA plate.It is that the grape ulcer caused by B.dothidea and L.theobromae etc. can effectively be prevented and treated by suspending agent prepared by effective ingredient by bacillus cereus BCJB01, the prevention effect of grape fruit ear Peptic Ulcers is reached more than 76.8%, the prevention effect of grape fruit Peptic Ulcers is reached more than 90.1%.
In the suspending agent of the present invention, effective active composition is the spore of bacillus cereus BCJB01, and in suspending agent, spore content is not less than 50 × 108Cfu/mL; in suspending agent, N-dodecyl-Beta-alanine sodium is auxiliary agent and dispersant; it has good resistance to highly basic, strong acid and electrolyte performance and the characteristic being readily biodegradable; carboxymethyl cellulose is thickening agent; other composition is protective agent; the activity of spore can be protected, extend the shelf-life of suspending agent.In suspending agent, the particle diameter of each component is not more than 5 μm.
Accompanying drawing illustrates:
Fig. 1 show as bacillus cereus BCJB01 antibacterial band size to B.dothidea on PDA plate；Fig. 2 show bacillus cereus BCJB01 antibacterial band size to L.theobromae on PDA plate.
Detailed description of the invention:
In order to be more fully understood that the present invention, describe technical scheme in detail with instantiation below, but the invention is not limited in this.
Embodiment 1
The flat board bacteriostasis of bacillus cereus BCJB01
Inoculate the pathogenic fungi truffle of diameter 5mm in PDA plate central authorities, inoculate bacillus cereus BCJB01,28 DEG C of cultivations at plate edge from 35mm place, center, record antibacterial bandwidth.
Result is shown in Figure of description, B.dothidea (Fig. 1) and L.theobromae (Fig. 2) is all had inhibitory action by bacillus cereus BCJB01 on PDA plate, obvious antibacterial band, antibacterial bandwidth respectively 7.5mm and 9.5mm can be produced.
Embodiment 2
Prepared by suspending agent
The fermentation liquid of preparation bacillus cereus BCJB01, fermentation ends spore content reaches 55～60 × 108Cfu/mL, prepares suspending agent in following ratio.
Fermentation liquid 1000mL
N-dodecyl-Beta-alanine sodium 20～25g
Carboxymethyl cellulose 3～5g
Sulphuric acid benzene sulfonic acid sodium salt 2～3g
2,6-dipicolinic acid calcium salt 0.1～0.2g
Peptidoglycan 0.1～0.2g.
In this suspending agent, effective active composition is the spore of bacillus cereus BCJB01, and in suspending agent, spore content is not less than 50 × 108Cfu/mL; in suspending agent, N-dodecyl-Beta-alanine sodium is auxiliary agent and dispersant; it has good resistance to highly basic, strong acid and electrolyte performance and the characteristic being readily biodegradable; carboxymethyl cellulose is thickening agent; other composition is protective agent; the activity of spore can be protected, extend the shelf-life of suspending agent.In suspending agent, the particle diameter of each component is not more than 5 μm.
Embodiment 3
The suspending agent control in field effect test to grape ulcer
Test site: community water source downy grape base, four, four, Luo Cheng county, Guangxi province town.Grape variety: downy grape.Test sets 3 process, and processing 1 is BCJB01 suspending agent, 100 times of diluents；Processing 2 is own azoles Fluoxastrobin, 60 times of diluents；Processing 3 to compare for clear water, each process sets 3 repetitions.Processing mode: dilution spraying.Administration time: on May 27th, 2015, on June 17th, 2015, on June 27th, 2015, spacing of about 7～10d, carries out according to weather condition.Control time: on August 25th, 2015.
Result of the test shows (table 1), the grape ulcer in four community water source downy grape bases, four, Luo Cheng county, Guangxi province town is had good prevention effect by suspending agent of the present invention, there is significant difference (p < 0.01) with compareing between medicament.Wherein the prevention effect of fruit ear is reached 76.8%, the prevention effect of fruit grain has been reached 90.1%.
The table 1 suspending agent of the present invention control in field effect to Guangxi Luo Cheng grape ulcer
| Process | Fruit ear sickness rate % | Fruit ear preventive effect % | Fruit grain sickness rate % | Fruit grain preventive effect % |
| Suspending agent of the present invention | 22.9±1.89A | 76.8 | 5.6±0.35A | 90.1 |
| Own azoles Fluoxastrobin | 71.6±2.66B | 27.5 | 30.3±7.10B | 46.9 |
| Clear water | 98.8±2.14C | - | 57.0±2.37C | - |
(note: the Duncan method in data acquisition SPSS10.0 statistical software is analyzed, result is 3 mean+SD repeated, and represents there is significant difference in 0.01 level between each process with different capitalizations.)
Embodiment 4
The suspending agent control in field effect test to grape ulcer
Experiment place: Zhang Shanying vineyard, Yanqing, Beijing.Grape variety: HONGTI.Test sets 3 process, and processing 1 is BCJB01 suspending agent, 100 times of diluents；Processing 2 is own azoles Fluoxastrobin, 60 times of diluents；Processing 3 to compare for clear water, each process sets 3 repetitions.Processing mode: dilution spraying.Administration time: on July 19th, 2015, medication 1 time, control time: on August 30th, 2015.
Result of the test shows (table 2), and the grape ulcer in Zhang Shanying vineyard, Yanqing, Beijing is had good prevention effect by suspending agent of the present invention, there is significant difference (p < 0.01) with compareing between medicament.Wherein the prevention effect of fruit ear is reached 85.7%, the prevention effect of fruit grain has been reached 94.1%.
The table 2 suspending agent of the present invention control in field effect to Yanqing County of Beijing grape ulcer
| Process | Fruit ear sickness rate % | Fruit ear preventive effect % | Fruit grain sickness rate % | Fruit grain preventive effect % |
| Suspending agent of the present invention | 11.6±0.81A | 85.7 | 1.9±0.36A | 94.1 |
| Own azoles Fluoxastrobin | 57.6±282B | 28.9 | 12.8±0.40B | 60.6 |
| Clear water | 81.1±1.90C | - | 32.5±1.86C | - |
(note: the Duncan method in data acquisition SPSS10.0 statistical software is analyzed, result is 3 mean+SD repeated, and represents there is significant difference in 0.01 level between each process with different capitalizations.)
Claims (9)
1. bacillus cereus BCJB01 application in preventing and treating grape ulcer.
2. application according to claim 1, it is characterised in that Fructus Vitis viniferae seat chamber bacterium and Lasiodiplodia theobromae are had inhibitory action by bacillus cereus BCJB01.
3. application according to claim 1, it is characterised in that bacillus cereus (Bacilluscereus) BCJB01, its preserving number is CGMCCNO.10477.
4. application according to claim 1, it is characterised in that bacillus cereus BCJB01 fermentation liquid is prepared into biocontrol agent and carries out the preventing and treating of grape ulcer.
5. application according to claim 4, it is characterized in that, described biocontrol agent is suspending agent, by bacillus cereus BCJB01 fermentation liquid, N-dodecyl-Beta-alanine sodium, carboxymethyl cellulose, sulphuric acid benzene sulfonic acid sodium salt, 2, dipicolimic acid 2 calcium and Peptidoglycan composition.
6. application according to claim 5, it is characterised in that described suspending agent consists of the following composition:
7. application according to claim 6, it is characterised in that in bacillus cereus BCJB01 fermentation liquid, spore content is for being not less than 55 × 108cfu/mL。
8. application according to claim 6, it is characterised in that in suspending agent, effective active component content is not less than 50 × 108cfu/mL。
9. application according to claim 6, it is characterised in that in suspending agent, each component particle diameter is not more than 5 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610141894.5A CN105767006B (en) | 2016-03-11 | 2016-03-11 | Applications of the bacillus cereus BCJB01 in terms of grape ulcer is prevented |
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| CN201610141894.5A CN105767006B (en) | 2016-03-11 | 2016-03-11 | Applications of the bacillus cereus BCJB01 in terms of grape ulcer is prevented |
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| CN105767006B CN105767006B (en) | 2018-05-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110616165A (en) * | 2019-05-14 | 2019-12-27 | 陕西省微生物研究所 | Bacillus cereus MA23, microbial inoculum, preparation method and application |
| US11058131B2 (en) | 2015-04-16 | 2021-07-13 | Kennesaw State University Research And Service Foundation, Inc. | Escherichia coli O157:H7 bacteriophage Φ241 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11058131B2 (en) | 2015-04-16 | 2021-07-13 | Kennesaw State University Research And Service Foundation, Inc. | Escherichia coli O157:H7 bacteriophage Φ241 |
| CN110616165A (en) * | 2019-05-14 | 2019-12-27 | 陕西省微生物研究所 | Bacillus cereus MA23, microbial inoculum, preparation method and application |
| CN110616165B (en) * | 2019-05-14 | 2022-06-28 | 陕西省微生物研究所 | Bacillus cereus MA23, microbial inoculum, preparation method and application |
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Effective date of registration: 20200521 Address after: 250000 28789 Licheng Road, Licheng District, Ji'nan, Shandong, 28789 Patentee after: ECOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES (THE SINO-JAPANESE FRIENDSHIP BIOTECHNOLOGY RESEARCH CENTER, SHANDONG ACADEMY OF SCIENCES) Address before: 250014 Shandong Province, Lixia District, Ji'nan City Hospital Road, No. 19 Patentee before: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES |