CN106591196B - One plant of photosynthetic bacteria and its application in prevention and treatment Cruciferae clubroot - Google Patents
One plant of photosynthetic bacteria and its application in prevention and treatment Cruciferae clubroot Download PDFInfo
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- CN106591196B CN106591196B CN201611252810.1A CN201611252810A CN106591196B CN 106591196 B CN106591196 B CN 106591196B CN 201611252810 A CN201611252810 A CN 201611252810A CN 106591196 B CN106591196 B CN 106591196B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The photosynthetic bacteria that can be applied to prevention and treatment crop in cruciferae clubroot the present invention relates to one plant and its application, belong to technical field of plant protection.The photosynthetic bacteria is Lampropedia Lampropedia sp., is named as photosynthetic bacteria GH-1, and depositary institution is China typical culture collection center, and collection deposit number is CCTCC NO:M 2016680, and the preservation time is on November 25th, 2016.Obtained strains of the present invention have the ability of efficient prevention and treatment crop in cruciferae clubroot, in prevention and treatment applied to crop in cruciferae clubroot, the disease incidence of clubroot can be reduced to 10% or so, and significantly reduce the disease index of plant, crop yield can be effectively improved simultaneously, rate of growth is up to 150% or more；Photosynthetic bacteria in the present invention is environmentally friendly, and use is safe, noresidue；The present invention has also widened the application field of photosynthetic bacteria.
Description
Technical field
The present invention relates to one plant of photosynthetic bacteria and its applications in prevention and treatment crop in cruciferae clubroot, belong to microorganism
And plant biological Prevention Technique field.
Background technique
Crop in cruciferae include rape and bulk vegetable, such as Chinese cabbage, wild cabbage, cauliflower, kohlrabi, leaf mustard, radish with
And special crop Radix Isatidis and horseradish, be China's plantation most extensively, eat most common vegetable species, in Zhejiang, wide in China
East, Guangxi, Sichuan, Anhui, Hunan, Jiangxi, Fujian, Jiangsu, Yunnan, Xinjiang, Tibet, Liaoning, Shandong, Shanghai, Beijing and
The area such as Chongqing is distributed.But crop in cruciferae is easy infection clubroot, and the area that clubroot occurs every year for China reaches
20000000 mu or more, and extremely difficult prevention and treatment, seriously affect the production and living and economic well-being of workers and staff of peasant.
Have at present to the main control measure that Cruciferae clubroot is taken: agricultural cultivation measure, disease-resistant variety, chemistry are anti-
Measure is controlled, but its control efficiency is undesirable.
Existing document report carries out prevention and treatment clubroot using biological control method, as 101416641 B of patent CN is reported
A kind of biological agent for preventing and treating Cruciferae clubroot, the production bacterial strain of the biological agent are bacillus amyloliquefaciens
(Bacillus subtilis) XF-1, the bacterial strain are obtained by liquid and solid fermentation culture, which is applied to ten
The prevention and treatment of Zi Hua section clubroot can be good at the generation for controlling clubroot, and production-increasing function is obvious, and control efficiency is reachable
84.8%.102899266 B of patent CN provides a kind of konjaku endogenetic bacteria pantoea agglomerans bacterial strain 1-7 and its is preparing cross
Application in flower section vegetables clubroot preparation, has the characteristics that efficient, nontoxic and growth-promoting effect is good.Patent CN 105296392
A, which reports one plant, has efficient prophylactic-therapeutic effect and the few biocontrol bacterial strain of administration dosage to rapeseed clubroot, which is solution starch
Bacillus Bam22 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
No.11329。
The prevention and treatment of crop in cruciferae clubroot is successfully applied to for photosynthetic bacteria there is not yet pertinent literature is reported at present.
Summary of the invention
Aiming at the shortcomings in the prior art, an object of the present invention, which is just to provide one plant, can be applied to prevention and treatment cruciate flower
The photosynthetic bacteria of section's clubroot can efficiently prevent and treat Cruciferae clubroot, reduce crop disease incidence, and can preferably promote crop
Volume increase.
The present inventor is by a large amount of experimental study and gropes, and obtains one plant of photosynthetic bacteria, the photosynthetic bacteria
For Lampropedia (Lampropedia sp.), it is named as photosynthetic bacteria GH-1 (Lampropedia hyaline GH-1),
China typical culture collection center, collection address are preserved on November 25th, 2016 are as follows: the Chinese Wuhan, the Wuhan is big
It learns, postcode: 430072, collection deposit number is CCTCC NO:M 2016680.
The second object of the present invention is to provide a kind of application of photosynthetic bacteria in prevention and treatment crop in cruciferae clubroot.
The third object of the present invention is to provide a kind of photosynthetic bacteria in the biology of preparation prevention and treatment crop in cruciferae clubroot
Application in terms of preparation.
Photosynthetic bacteria in the present invention includes following step when carrying out prevention and treatment crop in cruciferae clubroot as biological agent
It is rapid:
(1) biological agent containing the photosynthetic bacteria described in claim 1 is prepared；
(2) biological agent obtained by step (1) is administered using one of following methods: 1. admixes in base fertilizer or top dressing and applies
With；It is applied in seed 2. mixing；3. plus water is poured and is applied in crop root.
The concentration of the photosynthetic bacteria of the invention contained photosynthetic bacteria when preparing biological agent is 6~9 × 108cfu/mL.
Above-mentioned photosynthetic bacteria is applied to prevention and treatment crop in cruciferae clubroot, there is efficient prophylactic-therapeutic effect, it can be significant
The incidence of crop in cruciferae clubroot is reduced, and crop yield can be promoted, and environmentally friendly.As shown in the Examples, will
Photosynthetic bacteria of the invention is applied to prevent and treat the clubroot aspect of crop in cruciferae Chinese cabbage, the disease incidence of crop after preparation is made
It is reduced to 10% or so, average disease index is reduced to 9.4, and 1900 kilograms of crop yield is per acre.
The beneficial effects of the present invention are: obtained strains of the present invention have efficient prevention and treatment crop in cruciferae clubroot
Ability, in the prevention and treatment applied to Cruciferae clubroot, the disease incidence of clubroot can be reduced to 10% or so, and significantly reduce plant
The disease index of strain, while can effectively improve crop yield, and rate of growth is up to 150% or more；Photosynthetic bacteria in the present invention is to ring
Border is friendly, and use is safe, noresidue；The present invention has also widened the application field of photosynthetic bacteria.
Detailed description of the invention
Fig. 1 is the growth and development of seedling after 300 times of photosynthetic bacteria GH-1 preparation diluent post-processing Chinese cabbage seeds in embodiment 1
Comparative diagram；
Wherein, the side A is to handle using photosynthetic bacteria GH-1 preparation, and the side B is not handled using photosynthetic bacteria GH-1 preparation.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
1, experimental raw and method
1.1 bacterium source
Slight disease plant rhizosphere in the serious field of Guanghan clubroot morbidity.
1.2 culture medium
Culture medium prescription are as follows: peptone 10g, yeast extract 5g, sodium chloride 10g, water 1000mL, pH are adjusted to 5.0.
The preservation of 1.3 bacterial strains
By in 25% glycerol of strain inoculated, -70 DEG C or Liquid nitrogen storage, monthly culture transferring is primary.
The identification of 1.4 bacterial strains
The DNA for extracting bacterium carries out the analysis of 16S rDNA sequence by PCR method, and gene order is shown in sequence table.By gained sequence
It is listed in the website NCBI to be compared, and is named as photosynthetic bacteria GH-1.
Application of 1.5 bacterial strains in prevention and treatment crop in cruciferae clubroot
After obtained strains are prepared into test preparation, using root method is poured, direct root irrigation or dilute is carried out at seeding time
The method for releasing rear pouring root, the concentration of bacterial strain is 6~9 × 108cfu/mL in undiluted bacterium solution preparation, is poured every time with every plant
100mL bacterium solution carries out root irrigation, at least handles 2 times, carries out field efficiency test and simulation experiments respectively to Chinese cabbage and rape.
The severity Scaling standard and investigation method of Chinese cabbage and rapeseed clubroot carry out in the following manner:
Chinese cabbage severity Scaling standard are as follows:
0 grade: root is without tumour；
1 grade: main root enlargement, less than two times basal part of stem of diameter；
3 grades: main root enlargement, 2~3 times of a diameter of basal part of stem；
5 grades: main root enlargement, 3~4 times of a diameter of basal part of stem；
7 grades: there is blackening in main root enlargement, 4 times or more of a diameter of basal part of stem or the root of enlargement.
Rape severity Scaling standard are as follows:
0 grade: without tumour, root system development is normal for root；
1 grade: 1-30% root raw a little tumour, have small nodositas lump, be mutually not connected to；
2 grades: 31-60% root raw tumour, have big nodositas lump, be connected with each other；
3 grades: 61-100% root raw tumour, expand and form spindle tumor, big tumor mass extends to hypocotyl,
Plant is short and small, stops growing.
Disease index, disease incidence and control efficiency are calculated as follows:
Disease incidence (%)=(morbidity strain number/investigation total strain number) × 100；
(1) as follows to the test result of Chinese cabbage:
The controlling experiment of cabbage clubroot is used for using photosynthetic bacteria as biological agent, the results are shown in Table 1:
Table 1
It is used to prevent and treat the simulation experiments of cabbage clubroot using photosynthetic bacteria as biological agent, the results are shown in Table 2:
Table 2
| Processing | Yield (kg/ mus) | Increase production (kg/ mus) | Rate of growth (%) |
| CK | 900 | - | - |
| 100 times of dilution | 2800 | 1900 | 211.1 |
| 300 times of dilution | 2400 | 1500 | 166.7 |
| 500 times of dilution | 1950 | 1050 | 116.7 |
| 70% 500 times of Bravo | 1370 | 470 | 52.2 |
As can be seen from Table 1 and Table 2, the present invention is being prevented and treated using the biological agent containing photosynthetic bacteria for handling Chinese cabbage
Cabbage clubroot and Chinese cabbage volume increase aspect play good effect, and the preventive effect of 100 times of dilution is up to 87%, rate of growth
211.1%.
Root irrigation is carried out after photosynthetic bacteria preparation is diluted 300 times, to growth and development situation such as Fig. 1 institute of Chinese cabbage seedling
Show, wherein the side A is using photosynthetic bacteria preparation root irrigation, and the side B is control, it is seen that the Chinese cabbage growth of seedling of the side A is vigorous.
(2) as follows to the test result of rape:
The controlling experiment of rapeseed clubroot is used for using photosynthetic bacteria as biological agent, the results are shown in Table 3:
Table 3
It is used to prevent and treat the simulation experiments of rapeseed clubroot using photosynthetic bacteria as biological agent, the results are shown in Table 4:
Table 4
As can be seen from tables 3 and 4 that the preparation of the invention containing photosynthetic bacteria is in prevention and treatment rapeseed clubroot and rape yield increasing
Aspect plays good effect, and the preventive effect of 300 times of dilution is 80.4%, rate of growth 130.9%.
SEQUENCE LISTING
<110>Inst of Plant Protection, Sichuan Academy of Agricultural Sciences
<120>one plants of photosynthetic bacterias and its application in prevention and treatment Cruciferae clubroot
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1224
<212> DNA
<213>photosynthetic bacteria
<400> 1
gactgcaact gagtgtatcg ctccttgcgg tccgaacttc aggcaccccc ggcttccatg 60
gcttgacggg cggtgtgtac aaggcccggg aacgtattca ccgcgccatg gctgatgcgc 120
gattactagc gaatccaact tcacggagtc gagttgcaga ctccgatccg aactgagata 180
ggtttttgag atttgcgcac catcgctggt taccggccct ctgtacctac cattgtaaca 240
cgtgtgtcgc cccggacgta agggccgtgc tgatttgacg tcctcccccc cttcctcaca 300
ccttacggtg gcagtctcgt tagagttccc agcttgacct gatggcaact aacgacaagg 360
gttgcgctcg ttatggcact taagccgaca cctcacggca cgagctgacg acaaccatgc 420
agcacctcga tacacactat tgctagctat gatatctcta ccatattcct gcaccgttcg 480
agcccgggta aggttcctcg cgtatcatcg aattaaacca catgttcctc cgcttgtgcg 540
ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg tggataactt 600
atcgctttcg cttggtcact gactgtgtat cgccaacaac tagttatcat cgtttacagt 660
gtggactacc agggtatcta atcctgtttg atccccacac tttcgtgcct cagcgtcaat 720
aatggctttg tgagctgcct tcgcaatcga tattctgtgt gatatctaag catttcaccg 780
ctacaccaca cattccactc acagcaacca tattctagcc tgacagtatc aacggcattt 840
ttccagttaa gctgaaaact ttcaccactg acttatcaga ccgcctacgc accctttaaa 900
cccaataaat ccggataacg ctcgcatcct ccgtattacc gcggctgctg gcacggagtt 960
agccgatgct tattcatatg gtactctcat cagtcttcgc aaagacctta attgctccca 1020
tataaagcag tttacaatcc cataggaccg tcttcctgct acgcagcatg ctggatcaga 1080
ttcccatcca ttgtccgata tcctcactgc tgacctcctc gtagagtctg ggaccgtgtc 1140
tccagtccag tgtgggcgat cttcctctcg aatcctagac atctagtcat gtaggtcggt 1200
tacctacatc taacttaagt gtcc 1224
Claims (5)
1. one plant of photosynthetic bacteria, which is characterized in that the photosynthetic bacteria is Lampropedia (Lampropedia sp.), name
For photosynthetic bacteria GH-1, depositary institution are as follows: China typical culture collection center, collection number are as follows: CCTCC NO:M
2016680, the preservation time are as follows: on November 25th, 2016.
2. application of the photosynthetic bacteria described in claim 1 in prevention and treatment crop in cruciferae clubroot.
3. photosynthetic bacteria described in claim 1 is preparing answering in the biological agent for preventing and treating crop in cruciferae clubroot
With.
4. application according to claim 2, which is characterized in that the application includes the following steps:
(1) biological agent containing the photosynthetic bacteria described in claim 1 is prepared；
(2) biological agent obtained by step (1) is administered using one of following methods: 1. admixes in base fertilizer or top dressing and applies；
It is applied in seed 2. mixing；3. plus water is poured and is applied in crop root.
5. application according to claim 4, which is characterized in that in the biological agent photosynthetic bacteria concentration be 6~9 ×
108cfu/mL。
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| CN113249248B (en) * | 2021-04-25 | 2022-09-06 | 西华大学 | Gordonia melanodendri, application and pigment production method |
| CN113249274B (en) * | 2021-05-24 | 2022-03-11 | 齐鲁工业大学 | Junpian bacteria and junpian bacteria cell suspension and application thereof in preventing and treating pepper wilt |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN103667134A (en) * | 2013-12-10 | 2014-03-26 | 江苏省农业科学院 | Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof |
| CN105296392A (en) * | 2015-11-02 | 2016-02-03 | 四川省农业科学院植物保护研究所 | Fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae |
| CN105875649A (en) * | 2016-05-10 | 2016-08-24 | 长江师范学院 | Application of photosynthetic bacteria in tuber mustard clubroot prevention and control and tuber mustard clubroot prevention and control agent |
| CN105875648A (en) * | 2016-05-10 | 2016-08-24 | 长江师范学院 | Application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and tumorous stem mustard clubroot control agent |
-
2016
- 2016-12-30 CN CN201611252810.1A patent/CN106591196B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
| CN103667134A (en) * | 2013-12-10 | 2014-03-26 | 江苏省农业科学院 | Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof |
| CN105296392A (en) * | 2015-11-02 | 2016-02-03 | 四川省农业科学院植物保护研究所 | Fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae |
| CN105875649A (en) * | 2016-05-10 | 2016-08-24 | 长江师范学院 | Application of photosynthetic bacteria in tuber mustard clubroot prevention and control and tuber mustard clubroot prevention and control agent |
| CN105875648A (en) * | 2016-05-10 | 2016-08-24 | 长江师范学院 | Application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and tumorous stem mustard clubroot control agent |
Non-Patent Citations (5)
| Title |
|---|
| Lampropedia hyaline strain IAM 14890 16S ribosomal RNA, partial sequence，NCBI Reference Sequence: NR_040942.1;Xie C et al.;《Genbank》;20150203;第1-4页 * |
| 十字花科作物根肿病生防菌研究进展;彭丽莎等;《植物保护》;20151231;第41卷(第4期);第16-22页 * |
| 十字花科植物根肿病的生物防治及其病菌的分子检测技术研究;周青;《中国优秀硕士学位论文全文数据库 农业科技辑》;20140215(第2期);第D046-218页 * |
| 十字花科蔬菜根肿病及其综合防治技术;刘亚培等;《浙江农业科学》;20121231(第5期);第694-697页 * |
| 防治十字花科作物根肿病的油菜内生细菌分离与鉴定;王会军等;《中国油料作物学报》;20141231;第36卷(第1期);第92-97页 * |
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