CN105230664B - Applications of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot - Google Patents
Applications of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot Download PDFInfo
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- CN105230664B CN105230664B CN201510725788.7A CN201510725788A CN105230664B CN 105230664 B CN105230664 B CN 105230664B CN 201510725788 A CN201510725788 A CN 201510725788A CN 105230664 B CN105230664 B CN 105230664B
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Abstract
The present invention provides application processes of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot, and this method includes following method：Method one：By bacillus amyloliquefaciens Bam22 bacterium solution, root irrigation is carried out to crop in cruciferae with the amount of each every plant of 80 100 ml, is at least handled three times, processing time be after transplanting after the 1st day, the 7th day, the 15th day；Method two：By bacillus amyloliquefaciens Bam22 bacterium solutions, spraying treatment is carried out to crop in cruciferae with the amount of 150 200 ml of every square meter, is at least handled three times, processing time is the 1st day, the 7th day, the 14th day after planting.The application method of the present invention is simple, bacterium solution application concentration is small, bacterium solution administration dosage is few.
Description
Technical field
The invention belongs to the prevention and control fields of Cruciferae clubroot, and in particular to bacillus amyloliquefaciens Bam22 is being prevented
Application process in Cruciferae clubroot.
Background technology
Cruciferae clubroot is mainly in Europe, the U.S., Japan and southern area of China, and Cruciferae can be caused when serious
The total crop failure of crop causes huge puzzlement for agricultural production.
In recent years, due to higher and higher, the biological control for environmental-friendly requirement in control of crop disease work
Method is increasingly subject to the concern of the people, and has developed some and have the bacterial strain of preferable control effect to Cruciferae clubroot,
Such asBacillus subtilis KB-291、Bacillus subtilis Y10、Bacillus subtilis XF-1、Bacillus subtilis Bs2004、Bacillus subtilisM3.As above, the Cruciferae root of institute's development and application at present
Swollen diease occurrence bacteriological protection is mainly bacillus subtilis, bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Ten
Application in terms of the clubroot prevention of Zi Hua sections or blank, and the control effect of development and application bacterial strain is undesirable, it is still necessary to
Chemical agent coordinates the satisfactory prophylactic-therapeutic effect of competence exertion, while different degrees of there are access times more, application method
The drawbacks such as complexity constrain application development of the biocontrol microorganisms in terms of Cruciferae clubroot prevention.
The present inventor gropes to be found that a plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion by long-term a large amount of experiment
Bacterium bacillus amyloliquefaciens were preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on 6th in September in 2015
Center, preserving number are CGMCC No.11329.And how effectively using the bacterial strain come to prevent Cruciferae clubroot be very
It is necessary.
Invention content
The purpose of the present invention is to provide application sides of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot
Method, this method include following method：
Method one：By bacillus amyloliquefaciens Bam22 bacterium solutions, Cruciferae is made with the amount of each every plant of 80-100 ml
Object carries out root irrigation, at least handles three times, and processing time is the 1st day, the 7th day, the 15th day after planting；
Method two：By bacillus amyloliquefaciens Bam22 bacterium solutions, with the amount of every square meter 150-200 ml to crop in cruciferae
Spraying treatment is carried out, is at least handled three times, processing time is the 1st day, the 7th day, the 14th day after planting；
The crop in cruciferae includes rape, Chinese cabbage, broccoli；
The bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Bam22 is in the preservation on the 6th of September in 2015
In the Chinese microorganism strain preservation positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica
Administration committee's common micro-organisms center, preserving number are CGMCC No.11329.
It was found by the inventors of the present invention that bacillus bacillus amyloliquefaciens Bam22 is applicable not only to the processing side of pouring root
Method, while spraying treatment method can be also utilized under less dosage, there is huge actual use value.
Preferably, in the method one, the amount of application of bacterium solution is every plant of 100 ml in root irrigation.
Preferably, in the method two, the amount of application of bacterium solution is 180 ml per square meter in spraying treatment.
Preferably, in the method, the gemma amount of bacterium solution is 10 in pouring root and spraying treatment7 cfu/ml。
Preferably, the crop in cruciferae is rape.
Beneficial effects of the present invention：The application method of the present invention is simple, bacterium solution application concentration is small, bacterium solution administration dosage is few.
Specific implementation mode
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
Bacillus amyloliquefaciens Bam22 tests the control effect of Cruciferae clubroot
Experimental subjects：Rape, Chinese cabbage, broccoli.
After bacillus amyloliquefaciens Bam22 cultures 48h, 500 times are diluted, the following two kinds method is taken to be prevented：
Method one：By bacillus amyloliquefaciens Bam22 bacterium solutions, with the amount of each every plant of 100ml to crop in cruciferae into
Row root irrigation is at least handled three times, and processing time is the 1st day, the 7th day, the 15th day after planting；Note：Undiluted bacterium solution
Dense about 6-8 × 10 of bacterium9 cfu/ml。
Method two：By bacillus amyloliquefaciens Bam22 bacterium solutions, crop in cruciferae is carried out with the amount of every square meter 180ml
Spraying treatment is at least handled three times, and processing time is the 1st day, the 7th day, the 14th day after planting；Note：The bacterium of undiluted bacterium solution
Dense about 6-8 × 109 cfu/ml。
Severity Scaling：
0 grade：Without tumour, root system development is normal for root；
1 grade：The root of 1-30% a little tumour, has small nodositas lump, is mutually not connected to；
2 grades：The root of 31-60% raw tumour, there is big nodositas or spherical lump, is connected with each other；
3 grades：The root of 61-100% raw tumour, expands and forms spindle tumor, and big tumor mass extends to hypocotyl,
Plant is short and small, stops growing.
Disease index=[（Diseased plant numbers at different levels × opposite value of series）/ highest level × investigation total strain number] × 100.
Relative control effect=（Compare disease index-processing disease index）× 100/ control disease index.
Experimental result is shown in Table 1, table 2 and table 3：
1 rapeseed clubroot of table prevents result
2 cabbage clubroot of table prevents result
3 broccoli clubroot of table prevents result
Embodiment 2
Except bacterium solution amount of application is 80 every plant of ml in method one, in method two bacterium solution amount of application be 150ml per square meter it
Outside, remaining is consistent with embodiment 1, the low 2.0-2.5% of 1 gained of gained control effect average specific embodiment.
Embodiment 3
Except bacterium solution amount of application is 90 every plant of ml in method one, in method two bacterium solution amount of application be 200ml per square meter it
Outside, remaining is consistent with embodiment 1, and gained control effect is suitable with embodiment 1.
Claims (5)
1. application processes of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot, which is characterized in that the method
Including following method：
Method one：By bacillus amyloliquefaciens Bam22 bacterium solutions, crop in cruciferae is carried out with the amount of each every plant of 80-100ml
Root irrigation is at least handled three times, and processing time is the 1st day, the 7th day, the 15th day after planting；
Method two：By bacillus amyloliquefaciens Bam22 bacterium solutions, crop in cruciferae is carried out with the amount of every square meter 150-200ml
Spraying treatment is at least handled three times, and processing time is the 1st day, the 7th day, the 14th day after planting；
The one kind of the crop in cruciferae in rape, Chinese cabbage, broccoli；
High-efficiency cross-shaped flower section clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 was preserved in the micro- life of China on the 6th in September in 2015
Object culture presevation administration committee common micro-organisms center, preserving number CGMCCNo.11329.
2. according to the method described in claim 1, it is characterized in that, in the method one, the amount of application of bacterium solution in root irrigation
For every plant of 100ml.
3. according to the method described in claim 1, it is characterized in that, in the method two, the amount of application of bacterium solution in spraying treatment
It is the every square meters of 180ml.
4. according to the method described in claim 1, it is characterized in that, in the method, the bud of bacterium solution in pouring root and spraying treatment
Spore amount is 107cfu/ml。
5. according to the method described in claim 1, it is characterized in that, the crop in cruciferae is rape.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106399151B (en) * | 2016-07-22 | 2019-06-07 | 四川省农业科学院植物保护研究所 | One plant for preventing and treating the atrophy bacillus BA-7 of crop in cruciferae clubroot |
| CN106416835B (en) * | 2016-07-22 | 2019-06-07 | 四川省农业科学院植物保护研究所 | Utilize the method for bacillus atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot |
| CN107018995B (en) * | 2017-04-27 | 2019-10-11 | 四川省农业科学院植物保护研究所 | A method of organic tea yield is improved using biocontrol bacterial strain |
| CN107418910B (en) * | 2017-05-05 | 2020-08-25 | 昆明理工大学 | Biological agent for preventing and treating clubroot of cruciferous crops and application thereof |
| CN109699234A (en) * | 2018-08-30 | 2019-05-03 | 四川省农业科学院植物保护研究所 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
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| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
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| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
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