CN109699234A - A kind of seed-coating method and capsuled seed preventing rapeseed clubroot - Google Patents
A kind of seed-coating method and capsuled seed preventing rapeseed clubroot Download PDFInfo
- Publication number
- CN109699234A CN109699234A CN201910172284.5A CN201910172284A CN109699234A CN 109699234 A CN109699234 A CN 109699234A CN 201910172284 A CN201910172284 A CN 201910172284A CN 109699234 A CN109699234 A CN 109699234A
- Authority
- CN
- China
- Prior art keywords
- seed
- rape
- clubroot
- bacillus amyloliquefaciens
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to method fields, more particularly to a kind of seed-coating method and capsuled seed for preventing rapeseed clubroot, steps are as follows for coating method of the present invention: (1) bacillus amyloliquefaciens Bam22 bacterium solution is added in rape seed and is uniformly mixed, obtains soaking seed, it is spare；(2) filler and water are mixed in the ratio of solid-liquid mass ratio 1:0.3~0.5, it is spare；(3) step (2) resulting material is slowly added to soak in seed obtained by step (1) under stirring condition, be stirred evenly, air-dry to get.The survival rate that the present invention can be realized clubroot disease plant improves 70% or so, increases yield of rape；The growth and breeding that can inhibit other pathogenic microorganisms on the basis of inhibiting clubroot disease, is more advantageous to growth of rape；To soil, person poultry harmless, preparation is simple, is easy to large-scale use.
Description
Technical field
The present invention relates to method fields, and in particular to a kind of seed-coating method and pelleted seed for preventing rapeseed clubroot
Son.
Background technique
Clubroot is a kind of rape soil-borne disease caused by being infected by rape plasmodiophora brassicae, is conducive to disease in environmental condition
When, clubroot can lead to cole crop yield degradation and even have no harvest, and seriously threaten the production of rape.
Currently, the prevention and treatment for clubroot, mainly takes chemical prevention, crop rotation and soil improvement means.Soil improvement is (such as
A large amount of limings) although the effect of preferable prevention and treatment clubroot can be played, be easy to cause soil hardening and reduce soil
The adverse effect of fertility.Crop rotation method prevention and treatment clubroot is difficult, because Cruciferae host such as weeds etc. in soil can not be eliminated
Growth in dry farmland.Chemical prevention and control method is taken more and sprays chemical pesticide (such as cyazofamid, fluazinam) to be prevented and treated,
Chemical prevention is handled in addition to nursery soil chemicals treatment, seedling stage perfusion, it is also necessary in transplanting medicament pouring root, survive after medicament fill
The processing such as seedling can be only achieved preferable control efficiency；Simultaneously as pesticide dosage is big, the risk of environmental pollution is big, it is used for a long time
Chemical pesticide not only results in soil pollution, also increases the healthy and safe risk of people and animals；In addition, Control stage is too many, medicament
Cost is too high, and peasant household cannot receive.
Clubroot disease is not only present in soil of catching an illness, we are by for many years the study found that seed carries germ
It is the principal element that rapeseed clubroot takes place frequently, therefore, studies a kind of clubroot germ that rape seed can be inhibited self-contained
Early stage infect, and the seed of overwintering and latent clubroot germ in the soil of Infected regions directly infected can be prevented simultaneously
Early stage Simple treatment method is necessary.
Summary of the invention
In view of the above technical problems, the present invention provides a kind of seed-coating method and pelleted seed for preventing rapeseed clubroot
Son can be realized the catch an illness survival rate of seed plant of clubroot and improve 70% or so, and can increase yield of rape.
The technical solution adopted by the invention is as follows:
The present invention provides a kind of seed-coating method and capsuled seed for preventing rapeseed clubroot, and the method includes as follows
Step:
(1) bacillus amyloliquefaciens Bam22 bacterium solution is added in rape seed and is uniformly mixed, obtain soaking seed, it is spare；
(2) filler and water are mixed in the ratio of solid-liquid mass ratio 1:0.3~0.5, it is spare；
(3) step (2) resulting material is slowly added to soak in seed obtained by step (1) under stirring condition, stirring is equal
It is even, air-dry to get.
As a further optimization solution of the present invention, the bacillus amyloliquefaciens Bam22 is preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, deposit number is CGMCC No.11329, the deposit date is on September 6th, 2015,
Depositary institution address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and classification naming is bacillus amyloliquefaciens Bacillus
amyloliquefaciens。
As a further optimization solution of the present invention, it is 1 × 10 that the bacillus amyloliquefaciens Bam22 bacterium solution bacterium is dense9~5
×109cfu/mL。
As a further optimization solution of the present invention, the liquid of the bacillus amyloliquefaciens Bam22 bacterium solution and rape seed
Solid product mass ratio is 1:12~15.
As a further optimization solution of the present invention, the filler is kaolin and/or diatomite.
As a further optimization solution of the present invention, the mass ratio of the kaolin and diatomite is 1-2:1.
As a further optimization solution of the present invention, the filler quality is the 2%-5% of rape seed quality.
The present invention also provides the capsuled seeds being prepared by any of the above-described scheme.
Pathogen plasmodiophora can be attached in such a way that resting spore sticks on rape seed or with the soil that has plant residue
And carried out in non-decomposed dung it is overwintering, since pathogen plasmodiophora mode of infection and period are very special, except mainly by seedling stage it is direct
Infect rape root hair mode enter rape host plant and endangered outside, the mode of infection of rape plasmodiophora brassicae can also pass through
Seed-borne fungi mode aggravates the generation of clubroot.Particularly, once seed-borne fungi, then the risk of clubroot greatly increases,
Even if later period spraying pesticide, it can only also play " controlling " and not have the effect of " anti-", this is necessarily affected for Rape-seed production.
The present invention is activity with the microbial inoculum (i.e. bacillus amyloliquefaciens Bam22 bacterium solution) with Biological control function
Ingredient utilizes the occupy-place biological and ecological methods to prevent plant disease, pests, and erosion principle of " with bacterium bacterium ", forms a large amount of profitable strains in plant root, can achieve inhibition and kills
It goes out a variety of disinfect pathogens, the purpose of antagonism rape multiple diseases；Meanwhile viable bacteria breeding can also generate heat to a certain extent
The soil temperature around root system is improved, is conducive to the bundle soil of the loose and root system of plant of soil, the metabolite for secreting generation can be one
Determine the growth and development for stimulating rape in degree, and improve the physicochemical property of soil, promotes the decomposition of soil indissoluble nutrient, accelerate oil
Absorption of the dish to nutrient.Therefore, rape capsuled seed of the present invention, which has, promotes growth of rape, improves rape resistance, nontoxic, nothing
The characteristics of environmental pollution.
The present invention directly carries out rape seed to soak processing using bacillus amyloliquefaciens Bam22 bacterium solution, by beneficial
Mode is captured come the sprouting for the pathogen plasmodiophora resting spore for inhibiting rape seed to carry in the space of microorganism, blocks it to rape root
Hair infects, and can prevent infecting again for the original clubroot germ of soil simultaneously, and can inhibit other pathogenic microorganisms
Growth and breeding is more advantageous to growth of rape, reaches volume increase purpose, meanwhile, the bacterium solution is reinforced on seed using filler
Attachment, extends beneficial microbe bacterium solution to the action time of clubroot disease, enables bacterium solution to rape seed surface adhesion
Pathogen plasmodiophora and seed after planting pathogen plasmodiophora present in the surface of the seed soil carries out the prevention of continuous and effective, substantially reduce
Rapeseed clubroot incidence rate.
The advantages and benefits of the present invention are:
1, the survival rate that the present invention can be realized clubroot disease plant improves 70% or so；
2, bacillus amyloliquefaciens Bam22 bacterium solution of the present invention can inhibit other diseases on the basis of inhibiting clubroot disease
The growth and breeding of pathogenic microorganism, the metabolite for secreting generation can stimulate the growth and development of rape to a certain extent, reach increasing
Produce purpose；
3, to soil, person poultry harmless, preparation is simple, is easy to large-scale use.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Inventor, in Sichuan field trial, tests place in the western Gao Zhen in Guanghan City using capsuled seed produced by the present invention.
Embodiment 1
A kind of rape capsuled seed, is prepared by the following steps and obtains:
(1) prepare rape seed 15Kg, it is 1 × 10 that it is dense that 1L bacterium, which is added,9The bacillus amyloliquefaciens Bam22 bacterium solution of cfu/mL
It is uniformly mixed with rape seed, obtains soaking seed；
(2) 300g kaolin and 150mL water are mixed, under agitation by resulting material, is slowly added to soak rape
In seed, stir evenly, air-dry to get.
Embodiment 2
A kind of rape capsuled seed, is prepared by the following steps and obtains:
(1) prepare rape seed 15Kg, it is 3 × 10 that it is dense that 1L bacterium, which is added,9The bacillus amyloliquefaciens Bam22 bacterium solution of cfu/mL
It is uniformly mixed with rape seed, obtains soaking seed；
(2) 150g kaolin, 150g diatomite and 150mL water are mixed, under agitation by resulting material, is slowly added
Enter to soak in rape seed, stir evenly, air-dry to get.
Embodiment 3
A kind of rape capsuled seed, is prepared by the following steps and obtains:
(1) prepare appropriate rape seed 15Kg, it is 5 × 10 that it is dense that 1L bacterium, which is added,9The bacillus amyloliquefaciens Bam22 of cfu/mL
Bacterium solution is uniformly mixed with rape seed, obtains soaking seed；
(2) 200g kaolin, 100g diatomite and 100mL water are mixed, under agitation by resulting material, is slowly added
Enter to soak in rape seed, stir evenly, air-dry to get.
Embodiment 4
A kind of rape capsuled seed, is prepared by the following steps and obtains:
(1) prepare appropriate rape seed 12Kg, it is 1 × 10 that it is dense that 1L bacterium, which is added,9The bacillus amyloliquefaciens Bam22 of cfu/mL
Bacterium solution is uniformly mixed with rape seed, obtains soaking seed；
(2) 400g kaolin and 150mL water are mixed, under agitation by resulting material, is slowly added to soak rape
In seed, stir evenly, air-dry to get.
Experimental example 1
Using the rape capsuled seed Jing Guo 1~embodiment of embodiment 4 as experimental group；Control group 1 is artificial applies on a small quantity
Pathogen plasmodiophora is coated in rape seed and with 1 gained coating agent of embodiment；Control group 2 is without Cotton seeds, long root
Afterwards, the 1 times of liquid of dilution of bacillus amyloliquefaciens Bam22 bacterium solution described in appropriate embodiment 1 is being watered root week, control group 3 is benefit
Use common seed coat agent (sharp victory board method) as coating material, control group 4 is infected plant with no treatment, control group 5
For normal plant with no treatment.
Control efficiency result and yield result are shown in Table 1.
| Clubroot disease plant survival rate (%) | Yield (kg/ mus) | |
| Embodiment 1 | 88.8% | 179kg/ mus |
| Embodiment 2 | 90.2% | 198kg/ mus |
| Embodiment 3 | 91.5% | 215kg/ mus |
| Embodiment 4 | 89.7% | 189kg/ mus |
| Control group 1 | 88.5% | 173kg/ mus |
| Control group 2 | 78.9% | 168kg/ mus |
| Control group 3 | 50.7% | 146kg/ mus |
| Control group 4 | 20.3% | 92kg/ mus |
| Control group 5 | 20.3% | 157kg/ mus |
Claims (8)
1. a kind of seed-coating method for preventing rapeseed clubroot, which is characterized in that described method includes following steps:
(1) bacillus amyloliquefaciens Bam22 bacterium solution is added in rape seed and is uniformly mixed, obtain soaking seed, it is spare；
(2) filler and water are mixed in the ratio of solid-liquid mass ratio 1:0.3~0.5, it is spare；
(3) step (2) resulting material is slowly added to soak in seed obtained by step (1) under stirring condition, is stirred evenly, wind
It is dry to get.
2. the method according to claim 1, wherein the bacillus amyloliquefaciens Bam22 be preserved in China it is micro-
Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.11329.
3. the method according to claim 1, wherein the bacillus amyloliquefaciens Bam22 bacterium solution bacterium is dense is 1 ×
109~5 × 109cfu/mL。
4. the method according to claim 1, wherein in step (1), the bacillus amyloliquefaciens Bam22 bacterium
The liquid solid of liquid and rape seed product mass ratio is 1:12~15.
5. the method according to claim 1, wherein the filler is kaolin and/or diatomite.
6. according to the method described in claim 5, it is characterized in that, the mass ratio of the kaolin and diatomite is 1-2:1.
7. the method according to claim 1, wherein the filler quality is rape seed matter in step (2)
The 2%-5% of amount.
8. the capsuled seed being prepared by any one of claim 1-7 the method.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2018110044199 | 2018-08-30 | ||
| CN201811004419 | 2018-08-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109699234A true CN109699234A (en) | 2019-05-03 |
Family
ID=66266421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910172284.5A Pending CN109699234A (en) | 2018-08-30 | 2019-03-07 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109699234A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461349A (en) * | 2013-08-12 | 2013-12-25 | 四川省农业科学院植物保护研究所 | Seed coating agent, and preparation method and use thereof |
| CN104447071A (en) * | 2014-12-25 | 2015-03-25 | 刘长生 | Seed coating agent and coating method thereof |
| CN105053013A (en) * | 2015-08-28 | 2015-11-18 | 四川省农业科学院植物保护研究所 | Seed coating agent capable of efficiently preventing rape clubroot disease |
| CN105085071A (en) * | 2015-08-28 | 2015-11-25 | 四川省农业科学院植物保护研究所 | Sustained-release seed coating agent for efficiently preventing and curing rape clubroot |
| CN105230664A (en) * | 2015-11-02 | 2016-01-13 | 四川省农业科学院植物保护研究所 | Application of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae |
| CN105296392A (en) * | 2015-11-02 | 2016-02-03 | 四川省农业科学院植物保护研究所 | Fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN107418910A (en) * | 2017-05-05 | 2017-12-01 | 昆明理工大学 | A kind of biological agent for preventing and treating crop in cruciferae clubroot and its application |
| CN107668075A (en) * | 2017-09-28 | 2018-02-09 | 李宏 | A kind of coating bacteria agent for preventing and treating clubroot and preparation method thereof |
| CN108841745A (en) * | 2018-06-21 | 2018-11-20 | 慕恩（广州）生物科技有限公司 | One bacillus and its preparing microorganism seed coating in application |
-
2019
- 2019-03-07 CN CN201910172284.5A patent/CN109699234A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461349A (en) * | 2013-08-12 | 2013-12-25 | 四川省农业科学院植物保护研究所 | Seed coating agent, and preparation method and use thereof |
| CN104447071A (en) * | 2014-12-25 | 2015-03-25 | 刘长生 | Seed coating agent and coating method thereof |
| CN105053013A (en) * | 2015-08-28 | 2015-11-18 | 四川省农业科学院植物保护研究所 | Seed coating agent capable of efficiently preventing rape clubroot disease |
| CN105085071A (en) * | 2015-08-28 | 2015-11-25 | 四川省农业科学院植物保护研究所 | Sustained-release seed coating agent for efficiently preventing and curing rape clubroot |
| CN105230664A (en) * | 2015-11-02 | 2016-01-13 | 四川省农业科学院植物保护研究所 | Application of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae |
| CN105296392A (en) * | 2015-11-02 | 2016-02-03 | 四川省农业科学院植物保护研究所 | Fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae |
| CN107136123A (en) * | 2017-04-19 | 2017-09-08 | 明光市农源农作物专业合作社 | A kind of sodium alginate for preventing and treating clubroot is coated bacteria agent and preparation method thereof |
| CN107418910A (en) * | 2017-05-05 | 2017-12-01 | 昆明理工大学 | A kind of biological agent for preventing and treating crop in cruciferae clubroot and its application |
| CN107668075A (en) * | 2017-09-28 | 2018-02-09 | 李宏 | A kind of coating bacteria agent for preventing and treating clubroot and preparation method thereof |
| CN108841745A (en) * | 2018-06-21 | 2018-11-20 | 慕恩（广州）生物科技有限公司 | One bacillus and its preparing microorganism seed coating in application |
Non-Patent Citations (1)
| Title |
|---|
| 黄小琴 等: "解淀粉芽孢杆菌Bam22种子包衣防治油菜根肿病", 《中国植物保护学会2019年学术年会论文集》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100334201C (en) | Bacillus subtilis and its uses | |
| CN103975955B (en) | For preventing and treating compound bio seed coat agent and the preparation and application thereof of soil-borne diseases of vegetable | |
| CN102726454B (en) | Biological control agent for fusarium wilt and preparation and use method of biological control agent | |
| CN101124915B (en) | Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method | |
| CN101836640B (en) | Application of elicitor in controlling soil-borne diseases of garden crops | |
| CN103952329B (en) | Bacillus vallismortis and application thereof | |
| CN103667134B (en) | The Paenibacillus polymyxa N3-4 of one strain preventing and treating club-root and application thereof | |
| SU649293A3 (en) | Composition for destroying pathogenic microorganisms | |
| CN101058729B (en) | Soil conditioner for preventing and curing Cruciferae crop clubroot and preparation method thereof | |
| CN108083920A (en) | Microbial manure and its preparation method and application | |
| CN104311334A (en) | Composite improver for repairing heavy metal contaminated soil and preparation process | |
| CN107410364A (en) | A kind of microbial bacterial agent and its bacterial manure that can be used for preventing and treating root-knot nematode | |
| CN105028483B (en) | Composite bactericide and its application containing bacillus subtilis BAB 1 and Boscalid | |
| CN107164262A (en) | A kind of microbial bacterial agent and its bacterial manure available for controlling crop diseases and insect pests | |
| CN106754567B (en) | A kind of biological and ecological methods to prevent plant disease, pests, and erosion composite bacteria agent LAS of efficiently prevention various crop droop | |
| CN106431609B (en) | Ginseng Chinese yam peanut specific complex continuous cropping resisting biological microbial inoculum and preparation method thereof | |
| CN102533603A (en) | Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof | |
| CN107114411A (en) | For the trichoderma asperellum DQ3 compound powders YBY of raising rice seedlings and application | |
| CN106304894A (en) | Treating method of vegetable seeds | |
| CN109759441A (en) | A kind of alkaline land soil restorative procedure | |
| CN108849985B (en) | Ternary compound biological agent and application thereof in prevention and treatment of ginger bacterial wilt | |
| CN110074137A (en) | Application of the bacillus subtilis in prevention and treatment wheat aphid | |
| CN103299811B (en) | Method for enhancing soil waterflooding effect to control pepper phytophthora blight | |
| Phour et al. | Bioherbicidal potential of rhizosphere bacteria for management of Phalaris minor weed | |
| CN114250177B (en) | Acinetobacter and application thereof in improving stress resistance of plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190503 |
|
| RJ01 | Rejection of invention patent application after publication |