CN105230664A - Application of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae - Google Patents
Application of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae Download PDFInfo
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- CN105230664A CN105230664A CN201510725788.7A CN201510725788A CN105230664A CN 105230664 A CN105230664 A CN 105230664A CN 201510725788 A CN201510725788 A CN 201510725788A CN 105230664 A CN105230664 A CN 105230664A
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- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 24
- 241001503436 Plasmodiophora brassicae Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 claims abstract description 52
- 241000219193 Brassicaceae Species 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 238000003973 irrigation Methods 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims description 23
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 230000002262 irrigation Effects 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 5
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 claims description 4
- 235000017647 Brassica oleracea var italica Nutrition 0.000 claims description 4
- 240000003259 Brassica oleracea var. botrytis Species 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 3
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 3
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 230000003628 erosive effect Effects 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 238000010899 nucleation Methods 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000000443 biocontrol Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000003672 processing method Methods 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 101100381981 Caenorhabditis elegans bam-2 gene Proteins 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention provides an application method of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae. The method comprises the following two methods: the method I comprises: carrying out root-irrigation treatment on a cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 80-100 ml per time per strain, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fifteenth day after transplanting; the method II comprises: carrying out spraying treatment on the cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 150-200 ml per square meter, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fourteenth day after seeding. The bacillus amyloliquefaciens Bamm22 provided by the invention is simple in using method, the application concentration of the bacterial liquid is less and the application dosage of the bacterial liquid is less.
Description
Technical field
The invention belongs to the prevention and control field of Cruciferae club root, be specifically related to bacillus amyloliquefaciens Bam2
2application method in control Cruciferae club root.
Background technology
Cruciferae club root is mainly in Europe, the U.S., Japan and southern area of China, can cause the total crop failure of crop in cruciferae time serious, causes huge puzzlement for agricultural production.
In the last few years, due to more and more higher for eco-friendly requirement in control of crop disease work, biological control method received the concern of the people day by day, and have developed some have better control efficiency bacterial strain to Cruciferae club root, as
bacillussubtiliskB-291,
bacillussubtilisy10,
bacillussubtilisxF-1,
bacillussubtilisbs2004,
bacillussubtilism3.As above, the Cruciferae club root biocontrol bacteria of current institute Application and Development is mainly bacillus subtilis, bacillus amyloliquefaciens (
bacillusamyloliquefaciens) application in Cruciferae club root is prevented and treated or blank, and the control efficiency of Application and Development bacterial strain is undesirable, still need to coordinate the gratifying prophylactic-therapeutic effect of competence exertion with chemical agent, simultaneously in various degree to there are access times many, the drawbacks such as using method is complicated, constrain the application development of biocontrol microorganisms in Cruciferae club root is prevented and treated.
The present inventor gropes to have found a plant height effect Cruciferae club root biocontrol bacteria bacillus amyloliquefaciens through experiments a large amount of for a long time, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 6th, 2015, preserving number is CGMCCNo.11329.And how effectively to utilize this bacterial strain to be very necessary to prevent and treat Cruciferae club root.
Summary of the invention
The object of the present invention is to provide the application method of bacillus amyloliquefaciens Bam22 in control Cruciferae club root, the method comprises following method:
Method one: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out root irrigation with the amount of each every strain 80-100ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 15th day after planting;
Method two: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out spraying process with the amount of every square meter 150-200ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 14th day after planting;
Described crop in cruciferae comprises rape, Chinese cabbage, broccoli;
Described bacillus amyloliquefaciens (
bacillusamyloliquefaciens) Bam22 is preserved in the China Committee for Culture Collection of Microorganisms's common micro-organisms center being positioned at No. 3 Institute of Microorganism, Academia Sinica in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on September 6th, 2015, preserving number is CGMCCNo.11329.
The present inventor finds, bacillus bacillus amyloliquefaciens Bam22 is not only applicable to the processing method of filling with root, also can utilize spraying processing method simultaneously under less dosage, have huge actual use value.
Preferably, in described method one, in root irrigation, the amount of application of bacterium liquid is every strain 100ml.
Preferably, in described method two, in spraying process, the amount of application of bacterium liquid is the every square meter of 180ml.
Preferably, in described method, the gemma amount of filling with bacterium liquid in root and spraying process is 10
7cfu/ml.
Preferably, described crop in cruciferae is rape.
Beneficial effect of the present invention: using method of the present invention is simple, bacterium liquid application concentration is little, bacterium liquid application dosage is few.
Embodiment
Below by embodiment, the present invention is specifically described; what be necessary to herein means out is that following examples are just for being further detailed the present invention; limiting the scope of the invention can not be interpreted as; some nonessential improvement and adjustment that the person skilled in the art in this field makes according to foregoing invention content, still belong to protection scope of the present invention.
Embodiment 1
Bacillus amyloliquefaciens Bam22 tests the control efficiency of Cruciferae club root
Experimental subjects: rape, Chinese cabbage, broccoli.
After bacillus amyloliquefaciens Bam22 is cultivated 48h, dilute 500 times, take the following two kinds method to prevent and treat:
Method one: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out root irrigation with the amount of each every strain 100ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 15th day after planting; Note: the bacterium of undiluted bacterium liquid is dense is about 6-8 × 10
9cfu/ml.
Method two: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out spraying process with the amount of every square meter 180ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 14th day after planting; Note: the bacterium of undiluted bacterium liquid is dense is about 6-8 × 10
9cfu/ml.
Severity Scaling:
0 grade: root is without tumour, and root system development is normal;
The root of 1 grade: 1-30% a little tumour, has little nodositas lump, does not mutually connect;
The root of 2 grades: 31-60% raw tumour, has large nodositas or spherical lump, is interconnected;
The root of 3 grades: 61-100% raw tumour, and expand to formation spindle knurl, large knurl block extends to hypocotyl, and plant is short and small, stops growing.
Disease index=[(diseased plant number × relative level numerical value at different levels)/highest level × investigate total strain number] × 100.
Relative control effect=(contrast disease index-process disease index) × 100/ contrast disease index.
Experimental result is in table 1, table 2 and table 3:
Table 1 rape club root prevents and treats result
Table 2 cabbage clubroot prevents and treats result
Table 3 broccoli club root prevents and treats result
Embodiment 2
Except in method one, bacterium liquid amount of application is the every strain of 80ml, and in method two, bacterium liquid amount of application is that outside the every square meter of 150ml, all the other are consistent with embodiment 1, the low 2.0-2.5% of gained control efficiency average specific embodiment 1 gained.
Embodiment 3
Except in method one, bacterium liquid amount of application is the every strain of 90ml, and in method two, bacterium liquid amount of application is that outside the every square meter of 200ml, all the other are consistent with embodiment 1, and gained control efficiency is suitable with embodiment 1.
Claims (5)
1. the application method of bacillus amyloliquefaciens Bam22 in control Cruciferae club root, it is characterized in that, described method comprises following method:
Method one: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out root irrigation with the amount of each every strain 80-100ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 15th day after planting;
Method two: by bacillus amyloliquefaciens Bam22 bacterium liquid, carry out spraying process with the amount of every square meter 150-200ml to crop in cruciferae, at least process three times, the processing time is the 1st day, the 7th day, the 14th day after planting;
Described crop in cruciferae comprises rape, Chinese cabbage, broccoli;
Described high-efficiency cross-shaped flower section club root biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 6th, 2015, and preserving number is CGMCCNo.11329.
2. method according to claim 1, is characterized in that, in described method one, in root irrigation, the amount of application of bacterium liquid is every strain 100ml.
3. method according to claim 1, is characterized in that, in described method two, in spraying process, the amount of application of bacterium liquid is the every square meter of 180ml.
4. method according to claim 1, is characterized in that, in described method, the gemma amount of filling with bacterium liquid in root and spraying process is 10
7cfu/ml.
5. method according to claim 1, is characterized in that, described crop in cruciferae is rape.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510725788.7A CN105230664B (en) | 2015-11-02 | 2015-11-02 | Applications of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot |
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| CN201510725788.7A CN105230664B (en) | 2015-11-02 | 2015-11-02 | Applications of the bacillus amyloliquefaciens Bam22 in prevention Cruciferae clubroot |
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| CN105230664A true CN105230664A (en) | 2016-01-13 |
| CN105230664B CN105230664B (en) | 2018-09-28 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399151A (en) * | 2016-07-22 | 2017-02-15 | 四川省农业科学院植物保护研究所 | Bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops |
| CN106416835A (en) * | 2016-07-22 | 2017-02-22 | 四川省农业科学院植物保护研究所 | Method for preventing and controlling clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7 |
| CN107018995A (en) * | 2017-04-27 | 2017-08-08 | 四川省农业科学院植物保护研究所 | A kind of method that use biocontrol bacterial strain improves organic tea yield |
| CN107418910A (en) * | 2017-05-05 | 2017-12-01 | 昆明理工大学 | A kind of biological agent for preventing and treating crop in cruciferae clubroot and its application |
| CN109699234A (en) * | 2018-08-30 | 2019-05-03 | 四川省农业科学院植物保护研究所 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
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| CN102382781A (en) * | 2011-09-23 | 2012-03-21 | 湖北省生物农药工程研究中心 | Bacillus amyloliquefaciens for inhibiting bird rape plasmodiophora brassicae and separation and application of active substances thereof |
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2015
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Patent Citations (3)
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399151A (en) * | 2016-07-22 | 2017-02-15 | 四川省农业科学院植物保护研究所 | Bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops |
| CN106416835A (en) * | 2016-07-22 | 2017-02-22 | 四川省农业科学院植物保护研究所 | Method for preventing and controlling clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7 |
| CN106416835B (en) * | 2016-07-22 | 2019-06-07 | 四川省农业科学院植物保护研究所 | Utilize the method for bacillus atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot |
| CN106399151B (en) * | 2016-07-22 | 2019-06-07 | 四川省农业科学院植物保护研究所 | One plant for preventing and treating the atrophy bacillus BA-7 of crop in cruciferae clubroot |
| CN107018995A (en) * | 2017-04-27 | 2017-08-08 | 四川省农业科学院植物保护研究所 | A kind of method that use biocontrol bacterial strain improves organic tea yield |
| CN107018995B (en) * | 2017-04-27 | 2019-10-11 | 四川省农业科学院植物保护研究所 | A method of organic tea yield is improved using biocontrol bacterial strain |
| CN107418910A (en) * | 2017-05-05 | 2017-12-01 | 昆明理工大学 | A kind of biological agent for preventing and treating crop in cruciferae clubroot and its application |
| CN107418910B (en) * | 2017-05-05 | 2020-08-25 | 昆明理工大学 | Biological agent for preventing and treating clubroot of cruciferous crops and application thereof |
| CN109699234A (en) * | 2018-08-30 | 2019-05-03 | 四川省农业科学院植物保护研究所 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
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| CN105230664B (en) | 2018-09-28 |
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