CN104059855B - Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide - Google Patents
Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide Download PDFInfo
- Publication number
- CN104059855B CN104059855B CN201410206791.3A CN201410206791A CN104059855B CN 104059855 B CN104059855 B CN 104059855B CN 201410206791 A CN201410206791 A CN 201410206791A CN 104059855 B CN104059855 B CN 104059855B
- Authority
- CN
- China
- Prior art keywords
- kinds
- strains
- thalline
- microbial inoculum
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a composite fungicide containing following four bacterial strains: Epicoccum nigrum, Leptosphaerulina chartarum, Lecanicillium sp. and Peyronellaca pomorum. The fungicide can be used for treating heavy metal pollution of soil. According to the invention, the fungicide is prepared from mycothallus instead of bacteria, and the unit biomass of the fungicide is far higher than that of a bactericide, so that the fungicide is better in adsorption effect; in addition, the fungicide can be cultured in a solid state, and a finished product of the fungicide can be solid particles, so that the fungicide is more convenient to use.
Description
Technical field
The present invention relates to microorganism field, particularly relate to a kind of composite fungi system administering heavy metal pollution of soil
Agent and preparation method thereof.
Background technology
Along with the rapid expansion of human industryization process, the heavy metal pollution problem of soil is the current environment of China
The difficult problem that protection field one is the most urgently to be resolved hurrily.Heavy metal pollution of soil problem is essential, involve water source,
The normal use in soil, food safety, be related to Environmental Health, the great of people life property safety is asked
Topic.The large-area pollution in the whole nation, the pollution new region quickly occurred, pollution problem complicated and changeable make to pass
The Treatment process method of system is unable to reach satisfied regulation effect.It would therefore be desirable to one is more efficient,
Harmless, the advanced technology of low-maintenance with low cost, environmentally friendly.Along with people recognize for microbial world
Progressively deeply, scientist starts microorganism is used for Environment control, and this is more environmentally-friendly, low-carbon (LC), ecology
Friendly, merge natural method.
Traditional methods for curing heavy metal contamination in soil substantially belongs to physics, chemical category.Physical method includes
Soil replacement method, soil moved in improve the original method, electrodynamics method, thermal desorption method, extraction method etc..Soil replacement method is the top layer that will pollute
New free of contamination soil is changed after soil transfer；Soil moved in improve the original method be add in contaminated soil substantial amounts of pollution-free
Soil, covers in top layer or mixing.Electrodynamics method is at soil applying direct current electric field, by electrolysis, electricity
The effect such as migration, diffusion, electric osmose, electrophoresis makes heavy metal ion do relative motion, and at one end electrode will a huge sum of money
Belong to and collecting.Thermal desorption method carries out heat temperature raising to soil thus by heavy metal by being volatilized out soil environment.
Extraction method utilizes reagent and the heavy metal effect in soil, forms solable matter, then is rushed by pollutant with water
Outside root topsoil, add certain medicament and finally extract with heavy metal formation precipitation.Chemical method includes executing
Add modifying agent, add reductive organic matter etc..Modifying agent refers generally to alkaline matter (such as calcareous material),
Adding modifying agent makes soil pH value raise, and reduces the dissolubility of heavy metal such that it is able to reduce plant pair
Absorption in heavy metal in soil.Adding reducing agent can make some heavy metal in soil change valence state, dimension
Hold in the relatively low form of toxicity.Wherein, the characteristic repairing heavy metal pollution utilizing microorganism is current research
Focus, but major part research is all in initial stage, does not produce the ripe technical side that can be applicable to reality
Method.The most former (2009, application publication number: CN101838086) discloses a kind of biological preparation and with biological
Preparation processes the method for mud.This technology have employed Sucus Cocois and processes biological living with the mix preparation of calcium carbonate
Property mud.Sun Hongwen etc. (2009) disclose the Biological compound preparation that a kind of repairing heavy metal in soil pollutes.
This technology make use of the characteristic of the Adsorption of Heavy Metals of a kind of bacterial species Bacillus bacillus cereus, is hanged by this antibacterial
Liquid and a certain amount of humic acids add in fertilizer, serve fixing heavy metal and the effect in improvement farmland.
In the prior art, there is the problem that quantities is big and secondary pollution is serious in soil removal and replacement soil moved in improve the original method, therefore
It is only limitted to the IA that area is less；Electrodynamic force method power consumption is huge, and cost is prohibitively expensive；Thermal desorption method
Needing to provide a large amount of heat energy, therefore energy consumption is big, also substantially increases cost.Chemical method needs to add mostly
Substantial amounts of chemical agent, not only cost intensive, and easily produce a large amount of mud, cause secondary pollution.Raw
The growth cycle that phytoremediation in thing reparation needs is the longest, takes effect slow, simultaneously for different heavy metals
Choosing of super enriching plant is the most difficult.And utilize the mature technology of microbial treatments heavy metal pollution of soil
Seldom, patent is the most less.The most former patented technology make use of the internal microbiologic population self existed of mud,
Do not cultivate and produce specific microbe colony, therefore can be only applied in the mud containing microbe composition,
And other soil types cannot be applicable to, range of application is narrow.The patent of Sun Hongwen then make use of hay spore
Bacillus suspension, but its biomass and adsorption effect are unsatisfactory.
Therefore, there is a need in the art for biomass high, advantages of good adsorption effect, improvement soil weight easy to use
The microorganism formulation of metallic pollution.
Summary of the invention
It is an object of the invention to overcome the limitation of the traditional method of existing improvement heavy metal pollution of soil and not
Foot, it is provided that a kind of environmental protection, non-secondary pollution, with low cost, easy to use utilize composite fungi system
Technology and the preparation method of composite fungi preparation of heavy metal pollution of soil is administered in agent.
For reaching this purpose, the present invention by the following technical solutions:
In first aspect, the present invention provides a kind of composite fungi microbial inoculum, it is characterised in that described microbial inoculum contains
Epicoccum nigrum, Leptosphaerulina chartarum, Lecanicillium sp. and
Tetra-kinds of strains of Peyronellaca pomorum.
The described microbial inoculum of the present invention is solid.In the most described microbial inoculum, the particle diameter of thalli granule can be
1-3mm, more preferably 2mm.
In the microbial inoculum of the present invention, the mass ratio of described four kinds of strains can be 1:1:1:1:1.
In second aspect, the present invention provides the production method of microbial inoculum as described in relation to the first aspect, it is characterised in that
Described method includes cultivating described four kinds of strains respectively, the four kinds of strain mixing will cultivated afterwards.
In the production method of the described microbial inoculum of the present invention, the described method cultivating described four kinds of strains respectively can
To include:
A () first order seed is cultivated；
B () secondary seed is cultivated；
(c) amplification culture；
D () collects thalline；
E () optionally, thalline is dried；With
F () optionally, thalline is pulverized.
In the production method of the described microbial inoculum of the present invention, described amplification culture can be solid amplification culture,
The most described amplification culture can be carried out in the Solid agar culture based on glucose and peptone,
And preferably, described amplification culture can be carried out in large glass circle culture dish.
The production method of the described microbial inoculum of the present invention may include that
(1) described first order seed is cultivated and is cultivated for slant tube first order seed:
Preparing four groups of aseptic slant tube culture medium, its formula 1kg culture medium comprises: for glucose 18～23
G, peptone 8～12g, agar 12～16g, sodium chloride 0.1～0.3g, calcium chloride 0.1～0.2g, potassium chloride
0.3～0.6g, surplus is water, is seeded to respectively in each group of inclined-plane by the most isolated and purified described four kinds of strains
Carry out single culture, cultivation temperature 23 DEG C-27 DEG C, preferably 24 DEG C-26 DEG C, most preferably 25 DEG C, cultivate
4-6 days, preferably 4.5-5.5 days, more preferably 5 days, thalline covered with inclined-plane, obtained described four kinds respectively
The first order seed of strain；
(2) described secondary seed is cultivated and is cultivated for Petri dish secondary seed:
Preparing four groups of aseptic Petri Dish culture medium, its formula is containing anhydrous sodium acetate in 1L culture medium
0.24-0.26g, preferably 0.245-0.255g, more preferably 0.25g, yeast extract 0.12-0.16g, excellent
Selection of land 0.13-0.15g, more preferably 0.14g, agar 12-16g, preferably 13-15g, more preferably
14g, manganese chloride tetrahydrate 300-500 μm ol/L, preferably 350-450 μm ol/L, more preferably
400 μm ol/L, HEPES4.0-4.2g, preferably 4.05-4.15g, more preferably 4.1g, surplus for go from
Sub-water, pH is 5-7；
The first order seed of described four kinds of strains is accessed the most respectively, by described in four groups of culture medium
Four kinds of strains are individually cultivated under conditions of separating, cultivation temperature 23 DEG C-27 DEG C, preferably 24 DEG C
-26 DEG C, most preferably 25 DEG C, incubation time is 7～10 days, preferably 8-9 days, more preferably 8.5
My god, it is thus achieved that the secondary seed of described four kinds of strains；
(3) described amplification culture is large glass culture dish amplification culture:
Preparation culture medium, its formula is that 1kg culture medium comprises: for glucose 18～23g, peptone 8～12
G, agar 12～16g, sodium bicarbonate 0.15g, three water dipotassium hydrogen phosphate 0.35g, magnesium sulfate 0.05g, seven water
Ferrous sulfate 0.1g, sodium chloride 0.1～0.3g, calcium chloride 0.1～0.2g, potassium chloride 0.3～0.6g, surplus is
Water, makes sterile solid culture medium, and by described solid medium subpackage to large glass circle culture dish
In；
Spread cultivation inoculation: accesses two grades of kinds of described four kinds of strains the most respectively in four groups of culture medium
Son, takes described secondary seed fritter and accesses in large glass circle culture dish, and described four kinds of strains are dividing
Individually cultivating under conditions of from, cultivation temperature is 23 DEG C-27 DEG C, preferably 24 DEG C-26 DEG C, most preferably
25 DEG C of ground, incubation time is 9～14 days, preferably 10-13 days, more preferably 11-12 days, and pH is
5-8, preferably 6-7, more preferably 6.5；
(4) thalline is collected:
Aseptically, the thalline of the described four kinds of strains grown spreading cultivation is transferred to individually respectively
Aseptic clothes hanger；
(5) thalline is dried
Aseptically, the thalline on clothes hanger is dried by the natural air drying of drying baker, is dried temperature
Spending 20 DEG C-25 DEG C, preferably 21 DEG C-24 DEG C, more preferably 22 DEG C-23 DEG C, humidity is below 20%；
(6) thalline is pulverized
Aseptically, the thalline of described four kinds of strains of natural air drying is respectively put in sterile crushing machine
Carry out crushing operation, it is thus achieved that the pulverizing thalline of described four kinds of strains；
(7) mixing thalline
Take the pulverizing thalline of described four kinds of strains respectively, be sufficiently mixed, it is thus achieved that described microbial inoculum.
In the third aspect, the invention provides the microbial inoculum described in first aspect and administer the side of heavy-metal contaminated soil
Method, described method includes, will the invention provides the microbial inoculum described in first aspect and described heavy metal polluted soil
Earth contacts, and the most described heavy metal pollution is manganese metallic pollution.
In the method administering heavy-metal contaminated soil of the present invention, the consumption of described microbial inoculum can be 5g bacterium
More than agent/kg soil sample.
Beneficial effects of the present invention:
(1) present invention utilizes mycothallus rather than antibacterial, because the biomass of fungus is the biggest
In antibacterial, therefore the adsorption effect of the composite bacteria agent capable of the present invention is more preferably
(2) present invention can use solid state rheology, and the finished product manufactured can be solid particle, uses more
Convenient.
(3) comprising four kinds of funguses in the fungi preparation of the present invention, it cooperates and has the action effect of addition.
Accompanying drawing explanation
Fig. 1 is the difference microbial inoculum dosage hypothallus of the present invention adsorption efficiency variation diagram to cadmium.
Fig. 2 is the difference microbial inoculum dosage hypothallus of the present invention adsorption efficiency variation diagram to zinc.
Detailed description of the invention
Technical scheme is further illustrated below by detailed description of the invention.
The production method of embodiment 1. composite fungi preparation
(1) slant tube first order seed is cultivated:
Glucose 18g, peptone 8g, agar 12g, sodium chloride 0.1g, chlorine is comprised according to 1kg culture medium
Changing calcium 0.1g, potassium chloride 0.3g, surplus is the formula preparation slant tube culture medium of water.Make four groups of inclined-planes
Four kinds of the most isolated and purified strains are seeded in each group of inclined-plane individually train by Tube propagation base respectively
Support.Cultivation temperature controls at 23-25 DEG C, and after 4-6 days, thalline i.e. covers with inclined-plane.Slant culture terminate after examination
Pipe is placed on preservation in 4 DEG C of refrigerators.
(2) Petri dish secondary seed is cultivated:
Culture medium is prepared: containing anhydrous sodium acetate 0.24-0.26g, yeast extract in preparation 1L culture medium
0.12-0.16g, agar 12-16g, manganese chloride tetrahydrate 300-500 μm ol/L, HEPES4.0-4.2g, go from
Sub-water 1L, being adjusted to pH is 7.By culture medium sterilizing 30-40min under conditions of temperature 120 DEG C.From height
The culture medium solution taken out in temperature high-pressure sterilizing pot needs to be cooled to 60 DEG C in 60 DEG C of water-baths of constant temperature.Cooling
After culture medium take the 20mL subpackage disposable Corning to diameter 100mm height 20mm specification of equivalent
In circular culture dish, seal with sealed membrane after cooled and solidified, preserve under the conditions of 4 DEG C.
The cultivation of secondary seed: the Petri Dish culture medium equivalent produced is divided into four groups, at aseptic bar
Accessing four kinds of strains under part respectively in four groups of culture medium, four kinds of strains are individually trained under conditions of separating
Support.Cultivation temperature 24～27 DEG C, incubation time are 7～10 days, and culture medium bacterium state smooth surface is without dye
Bacterium.
(3) large glass culture dish amplification culture:
Culture medium is prepared: glucose 18g, peptone 8g, agar 12g, sodium bicarbonate 0.15g, three water
Dipotassium hydrogen phosphate 0.35g, magnesium sulfate 0.05g, ferrous sulfate heptahydrate 0.1g, sodium chloride 0.1g, calcium chloride
0.1g, potassium chloride 0.3g, make solid medium, and every bottled amount is about 1Kg, sterilizing at 130 DEG C
Sterilization 30min.The culture medium solution taken out from autoclave sterilization pot needs cold in 60 DEG C of water-baths of constant temperature
But to 60 DEG C.Culture medium after cooling takes the 70mL subpackage of equivalent to diameter 200mm height 30mm specification
Large glass circle culture dish in, after cooled and solidified with sealed membrane seal, under the conditions of 4 DEG C preserve.
Spread cultivation inoculation: the large glass circle culture medium equivalent produced is divided into four groups, aseptically
In four groups of culture medium, access four kinds of strains respectively, first order seed strain is taken fritter and accesses large glass circle
In shape culture dish, four kinds of strains are individually cultivated under conditions of separating.Cultivation temperature be 25～
27 DEG C, incubation time is 9～14 days, and pH is about 5～8, after testing without entering at next step after miscellaneous bacteria
Reason.
(4) Fungal biodiversity is collected:
In environment after stringent sterilization sterilization (again through ultraviolet disinfection 30min after ozonization 2h), will
The thalline grown that spreads cultivation in large glass culture dish is transferred to aseptic plastic clothes hanger, every kind of fungus strain
Collection process is consistent, but needs to arrange clothes hanger individually, during stop cross-contamination, whole process
Keep aseptic cleaning.
(5) thalline is dried
In sterilizing room, the thalline being placed on clothes hanger is made up to dry shape by the natural air drying of drying baker
State.Four kinds of strains are respectively equipped with independent drying baker.Whole dry run keeps aseptic cleaning, room ventilation
Filtration through air filter, it is ensured that sterile air free from admixture granule.Temperature controls at 20-25 DEG C, nothing
Need extra heating system.Humid control is below 20%.
(6) thalline is pulverized
The thalline of the four of natural air drying kinds of strains is entered in sterile crushing machine and carries out crushing operation.After pulverizing
Grain diameter at about 1-3mm.
(7) canned and storage
(i.e. the mass ratio of four kinds of strains is 1:1:1:1, respectively to take the pulverizing thalline of four kinds of strains of equivalent respectively
Account for the 25% of total amount), it is sufficiently mixed, uses aseptic wide-mouth brown glass reagent bottle to carry out thalli granule
Canned.It is placed on lucifuge after bottling, is dried, preserves under room temperature condition.
Embodiment 2. uses the composite fungi preparation of the present invention to process the pedotheque containing high concentration manganese
The composite fungi preparation of the present invention is applied in the pedotheque containing high concentration manganese of the Chongqing, obtain good
Regulation effect (table 1).The consumption of composite fungi preparation is 5g preparation/kg soil sample.The soil of pollution by manganese is taken from
The on-site topsoil of Chongqing manganese ore, takes the soil in place everywhere altogether as sample, contains everywhere in soil
The concentration of manganese difference.As it can be seen from table 1 the composite fungi preparation of the present invention is dense for Manganese In Soils
The tolerance range of degree and subject range are relatively wide, either～10mg/kg or～100mg/kg containing manganese concentration
Can play good effect, clearance has all reached more than 96%.
Table 1: the composite fungi preparation of the present invention is for the regulation effect containing wad earth
Embodiment 3. uses the composite fungi preparation of the present invention to process the pedotheque containing high concentration cadmium zinc
Weighing by each 5g of cadmium pollution soil sample is blank, prepares 4 groups of composite fungi preparations simultaneously and processes
Sample, often group sample weight 5g Han soil sample, the most respectively interpolation microbial bacteria liquid measure than for 20ml,
30ml, 50ml, 100ml, be adjusted to 6.0 by the pH value of all samples, and the regulation of soil pH value uses
1mol/L HCl or 1mol/L NaOH, incubated at room temperature.
Test result indicate that, these four fungus strain the composite bacteria agent capable being mixed is in soil
Cadmium and zinc have the strongest fixation.After adding different amounts of microbial inoculum, the cadmium zinc in the soil liquid
Concentration has decline (table 2) significantly compared with blank.Along with the increase of microbial inoculum dosage,
Thalline decreases (Fig. 1,2) to the adsorption efficiency of cadmium zinc.Particularly, 20-50mL scope is added
Microbial inoculum time, thalline is higher to the adsorption efficiency of cadmium, is maintained at about 95%.
Table 1: remaining cadmium Zn content in the soil liquid under different microbial inoculum dosages
Applicant states, the present invention illustrates detailed features and the method for the present invention by above-described embodiment,
But the invention is not limited in above-mentioned detailed features and method, i.e. do not mean that the present invention has to rely on above-mentioned
Detailed features and method could be implemented.Person of ordinary skill in the field is it will be clearly understood that to the present invention's
Any improvement, the equivalence to material selected by the present invention and step is replaced and assists material and the increasing of step
Add, concrete way choice etc., all fall within protection scope of the present invention and open within the scope of.
Claims (12)
1. a composite fungi microbial inoculum, it is characterised in that described microbial inoculum contains epicoccum nigrum (Epicoccum
Nigrum), its deposit number is CGMCC No.5775, Leptosphaerulina chartarum, and it is protected
Hiding numbered CGMCC No.5776, Lecanicillium sp., its deposit number is CGMCC No.6337
With Peyronellaca pomorum, its deposit number is tetra-kinds of strains of CGMCC No.6338；Described four kinds
The mass ratio of strain is 1:1:1:1～3:3:2:1.
Microbial inoculum the most according to claim 1, it is characterised in that described microbial inoculum is solid.
Microbial inoculum the most according to claim 1, it is characterised in that the particle diameter of thalli granule in described microbial inoculum
For 1-3mm.
4. the production method of the microbial inoculum described in claim 1, it is characterised in that described method includes respectively
Cultivate described four kinds of strains, the four kinds of strain mixing will cultivated afterwards.
Method the most according to claim 4, it is characterised in that described cultivate described four kinds of bacterium respectively
The method planted includes:
A () first order seed is cultivated；
B () secondary seed is cultivated；
(c) amplification culture；
D () collects thalline；
E () thalline is dried；With
F () thalline is pulverized.
Method the most according to claim 5, it is characterised in that described amplification culture is that solid expands
Cultivate.
Method the most according to claim 6, it is characterised in that described amplification culture with glucose and
Peptone is to carry out in main Solid agar culture.
Method the most according to claim 6, it is characterised in that described amplification culture is at large glass circle
Shape culture dish is carried out.
Method the most according to claim 5, it is characterised in that
(1) described first order seed is cultivated and is cultivated for slant tube first order seed:
Preparing four groups of aseptic slant tube culture medium, its formula 1kg culture medium comprises: for glucose 18～23
G, peptone 8～12g, agar 12～16g, sodium chloride 0.1～0.3g, calcium chloride 0.1～0.2g, potassium chloride
0.3～0.6g, surplus is water, is seeded to respectively in each group of inclined-plane by the most isolated and purified described four kinds of strains
Carrying out single culture, cultivation temperature 23 DEG C-27 DEG C, cultivate 4-6 days, thalline covers with inclined-plane, obtains institute respectively
State the first order seed of four kinds of strains；
(2) described secondary seed is cultivated and is cultivated for Petri dish secondary seed:
Preparing four groups of aseptic Petri Dish culture medium, its formula is containing anhydrous sodium acetate in 1L culture medium
0.24-0.26g, yeast extract 0.12-0.16g, agar 12-16g, manganese chloride tetrahydrate
300-500 μm ol/L, HEPES 4.0-4.2g, surplus is deionized water, and pH is 5-7；
The first order seed of described four kinds of strains is accessed the most respectively, by described in four groups of culture medium
Four kinds of strains are individually cultivated under conditions of separating, cultivation temperature 23 DEG C-27 DEG C, incubation time be 7～
10 days, it is thus achieved that the secondary seed of described four kinds of strains；
(3) described amplification culture is large glass culture dish amplification culture:
Preparation culture medium, its formula is that 1kg culture medium comprises: for glucose 18～23g, peptone 8～12
G, agar 12～16g, sodium bicarbonate 0.15g, three water dipotassium hydrogen phosphate 0.35g, magnesium sulfate 0.05g, seven water
Ferrous sulfate 0.1g, sodium chloride 0.1～0.3g, calcium chloride 0.1～0.2g, potassium chloride 0.3～0.6g, surplus is
Water, makes sterile solid culture medium, and by described solid medium subpackage to large glass circle culture dish
In；
Spread cultivation inoculation: accesses two grades of kinds of described four kinds of strains the most respectively in four groups of culture medium
Son, takes described secondary seed fritter and accesses in large glass circle culture dish, and described four kinds of strains are dividing
Individually cultivating under conditions of from, cultivation temperature is 23 DEG C-27 DEG C, and incubation time is 9～14 days, pH
For 5-8；
(4) thalline is collected:
Aseptically, the thalline of the described four kinds of strains grown spreading cultivation is transferred to individually respectively
Aseptic clothes hanger；
(5) thalline is dried
Aseptically, the thalline on clothes hanger is dried by the natural air drying of drying baker, is dried temperature
Spending 20 DEG C-25 DEG C, humidity is below 20%；
(6) thalline is pulverized
Aseptically, the thalline of described four kinds of strains of natural air drying is respectively put in sterile crushing machine
Carry out crushing operation, it is thus achieved that the pulverizing thalline of described four kinds of strains；
(7) mixing thalline
Take the pulverizing thalline of described four kinds of strains respectively, be sufficiently mixed, it is thus achieved that described microbial inoculum.
10. use the method that heavy-metal contaminated soil administered by the microbial inoculum according to any one of claim 1-3,
Described method includes, is connect with described heavy-metal contaminated soil by the microbial inoculum according to any one of claim 1-3
Touch.
11. methods according to claim 10, it is characterised in that described heavy-metal contaminated soil is manganese
Metallic pollution soil.
12. methods according to claim 10, the consumption of wherein said microbial inoculum is 5g microbial inoculum/kg soil sample
Above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206791.3A CN104059855B (en) | 2014-05-16 | 2014-05-16 | Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410206791.3A CN104059855B (en) | 2014-05-16 | 2014-05-16 | Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104059855A CN104059855A (en) | 2014-09-24 |
| CN104059855B true CN104059855B (en) | 2017-01-11 |
Family
ID=51547790
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410206791.3A Expired - Fee Related CN104059855B (en) | 2014-05-16 | 2014-05-16 | Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104059855B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105312317B (en) * | 2015-05-08 | 2018-08-17 | 中国环境科学研究院 | A method of acidity is repaired by petroleum hydrocarbon, heavy-metal contaminated soil using saccharomycete is strengthened |
| CN105296359A (en) * | 2015-08-21 | 2016-02-03 | 浙江中烟工业有限责任公司 | Endophytic fungus strain NYN771C06 and application thereof |
| CN106591156B (en) * | 2017-01-16 | 2019-07-16 | 昆明理工大学 | One plant of epicoccum nigrum FXZ2 and its application |
| CN108405603B (en) * | 2018-02-07 | 2020-09-29 | 深圳市深港产学研环保工程技术股份有限公司 | Method for leaching heavy metal contaminated soil by using microbial preparation |
| CN110221041B (en) * | 2019-06-06 | 2022-01-21 | 生态环境部南京环境科学研究所 | Method for detecting heavy metal contaminated soil ecotoxicity by using luminous earthworms |
| CN110577909A (en) * | 2019-09-17 | 2019-12-17 | 合肥师范学院 | method for preparing efficient phosphate solubilizing epicoccum with heavy metal tolerance characteristic |
| TWI761751B (en) * | 2020-01-14 | 2022-04-21 | 明志科技大學 | Method and equipment for removing contaminants by using mixed bacterial liquid to enhance electric power |
| CN112358974B (en) * | 2020-12-09 | 2022-06-10 | 昆明理工大学 | Plant endophytic fungus epicoccum nigrum FZT214 and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690022B (en) * | 2012-05-31 | 2014-06-04 | 中节能六合天融环保科技有限公司 | Upflow microbial reactor processing technology for manganese-bearing waste water |
| CN102994393B (en) * | 2012-08-28 | 2014-09-17 | 中节能六合天融环保科技有限公司 | Fungal strain LP-19-3 and application of fungal strain LP-19-3 in copper-containing wastewater treatment |
| CN102994394B (en) * | 2012-08-28 | 2014-12-31 | 中节能六合天融环保科技有限公司 | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment |
-
2014
- 2014-05-16 CN CN201410206791.3A patent/CN104059855B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104059855A (en) | 2014-09-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104059855B (en) | Composite fungicide for treating heavy metal pollution of soil and preparation method of composite fungicide | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN107254427A (en) | A kind of Bei Laisi Bacillus strains JN5 and its application | |
| CN102643760B (en) | Antagonistic bacterium capable of generating siderophore for controlling plant diseases | |
| CN104911127B (en) | A kind of rhizobium and its microbial inoculum and preparation method and application | |
| CN106493167A (en) | Bacillus licheniformis and microbial inoculum and their application and heavy metal-passivated method | |
| CN112358974B (en) | Plant endophytic fungus epicoccum nigrum FZT214 and application thereof | |
| CN105219668A (en) | A kind of heavy metal-polluted soil activation microbial inoculum and preparation method thereof and application | |
| CN101735955B (en) | Method for producing simple penicillium and metabolins thereof and application thereof | |
| CN110184225B (en) | Rhizosphere growth-promoting bacterium PHE-2 with PAHs degradation capacity and application thereof | |
| CN104630087B (en) | A kind of Rhizosphere of Crops Promoting bacteria YM4 and its application | |
| CN105112057A (en) | Soil heavy metal repairing agent, preparation thereof and application | |
| CN109504622A (en) | A kind of lysine bacillus and the composite bacteria agent of bacillus subtilis and preparation method thereof | |
| CN104630090B (en) | A kind of Rhizosphere of Crops Promoting bacteria YM3 and its application | |
| CN104911169B (en) | A kind of method for training cordyceps mycelium and fibrinolysin processed | |
| CN104673715A (en) | Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli | |
| CN108587947A (en) | One plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement | |
| CN104017749A (en) | Endogenous bacillus subtilis positive mutant strain, preparation method thereof, prepared biocontrol agent and application of biocontrol agent in preventing and controlling pomegranate dry rot | |
| CN105505843B (en) | One plant of Photosynthetic bacterium strain, the Liquid Fertilizer containing the bacterial strain and preparation method, application | |
| CN106544308A (en) | A kind of compost fermentation powder with guarantor's nitrogen function and preparation method thereof | |
| CN104263682A (en) | Plant-growth-promoting endophytic bacterium having polycyclic aromatic hydrocarbons degrading function and application thereof | |
| CN106434490A (en) | Ginseng bacterium TY15-2 with effects of disease prevention and growth promotion and application thereof | |
| CN109516869A (en) | Complex function microorganism formulation and its preparation method and application | |
| CN103396969A (en) | Fermentation culture medium capable of mixedly culturing brevibacillus laterosporus and bacillus subtilis and fermentation method thereof | |
| CN105907399B (en) | A kind of cadmium pollution renovation agent and its with the application in plant combined reparation agricultural land soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PP01 | Preservation of patent right |
Effective date of registration: 20200908 Granted publication date: 20170111 |
|
| PP01 | Preservation of patent right | ||
| PD01 | Discharge of preservation of patent |
Date of cancellation: 20220908 Granted publication date: 20170111 |
|
| PD01 | Discharge of preservation of patent | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170111 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |