CN105219679B - One plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 - Google Patents
One plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 Download PDFInfo
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- CN105219679B CN105219679B CN201510726834.5A CN201510726834A CN105219679B CN 105219679 B CN105219679 B CN 105219679B CN 201510726834 A CN201510726834 A CN 201510726834A CN 105219679 B CN105219679 B CN 105219679B
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- clubroot
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- pests
- bacillus amyloliquefaciens
- cruciferae
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- 201000010099 disease Diseases 0.000 title claims abstract description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 13
- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 10
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 8
- 230000003628 erosive effect Effects 0.000 title claims abstract description 8
- 244000005700 microbiome Species 0.000 claims abstract description 5
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- 230000001580 bacterial effect Effects 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 8
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- 230000000443 biocontrol Effects 0.000 description 8
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- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 2
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 241001503464 Plasmodiophora Species 0.000 description 2
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- 239000005754 Cyazofamid Substances 0.000 description 1
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Abstract
The present invention provides a plant heights to imitate Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22, it is characterized in that, high-efficiency cross-shaped flower section's clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on September 6th, 2015, and deposit number is CGMCC No.11329.Bacterial strain of the present invention has efficient protection effect to Cruciferae clubroot, and application bacterial concentration is low, dosage is few, has very huge actual application prospect.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to a plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion solution starch bud
Spore bacillus Bam22.
Background technique
Cruciferae clubroot is a kind of worldwide disease caused by being infected by plasmodiophora brassica bacteria, in Europe, the U.S. plus is taken
Greatly, Japan and other countries area is in spreading trend, seriously threatens the production of crop in cruciferae.
The Cruciferae clubroot principal pathogenetic in China is in East China and South China.Since the nineties in last century, cross
Flower section's clubroot starts to spread to Sichuan.Even to this day, only the crop in cruciferae in Chengdu is caused by the infringement of clubroot
Economic loss have reached annual more than one hundred million yuans.In addition, rape, as important oil crops, the threat faced is especially tight
Weight.Therefore, the method for finding efficiently prevention and treatment Cruciferae (especially rape) clubroot seems very necessary.
Currently, the method for prevention and treatment Cruciferae clubroot is mainly chemical prevention method, such as utilize cyazofamid, fluazinam
It learns to farm medicine.However, application chemical pesticide not only influences soil reclamation, while there is also cannot be neglected food for a long time apparently
Safety problem.In recent years, sight is placed on environmental-friendly measure of biotic control by researcher, it was found that some pairs of clubroots have
The bacterium of preferable control efficiency, such asBacillus subtilis KB-291、Bacillus subtilis Y10、Bacillus subtilis XF-1、Bacillus subtilis Bs2004、Bacillus subtilis M3。
But, although having obtained certain progress in the research of biocontrol bacterial strain, there are still asking at least three aspects
Topic: (1) exclusive use biocontrol bacterial strain control efficiency is bad, often needs to be combined with chemical agent, limits biocontrol bacterial strain in cross
Further applying in flower section's clubroot preventing and controlling；(2) administration dosage is big, is unfavorable for the colony balance of soil, if applying for a long time
With might have not expected influence；(3) it since plasmodiophora brassicae is unable to the characteristic of in vitro culture, is not yet formed at present high
The definite theory of effect exploitation clubroot biocontrol bacterial strain, therefore the bacterial strain developed is few, although knowing some bacillus to clubroot
Prevention and treatment it is effective, but the bacillus for having certain effect that developed also is no more than 10 plants, and predominantly bacillus subtilis
Bacterium, control efficiency not yet comply with one's wishes.
It would therefore be highly desirable to develop a kind of pair of Cruciferae (especially rape) clubroot with efficient prophylactic-therapeutic effect and apply
The few biocontrol bacterial strain of dosage.
Summary of the invention
In view of the shortcomings of the prior art, the purpose of the present invention is to provide a plant heights to imitate Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion Xie Dian
Afnyloliquefaciens Bam22, the bacillus were preserved on September 6th, 2015 positioned at Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
The China Committee for Culture Collection of Microorganisms's common micro-organisms center of number Institute of Microorganism, Academia Sinica, deposit number are
CGMCC No.11329。
Bacterial strain of the present invention has the following characteristics that (1) bacterium colony is rounded or similar round, has fold, slightly swells, opaque, in white
Color, the aobvious positive of Gram's staining, microscopically observation is in rod-short；(2) there is field planting property well to a variety of Cruciferae, it is right
The sprouting of plasmodiophora brassica bacteria resting spore has extraordinary inhibiting effect, can efficiently prevent and treat a variety of Cruciferae (especially rape)
Clubroot.
The present inventor separates by prolonged edaphon and research work, in numerous bacillus
It has found bacterial strain of the present invention, and finds that it has very efficient control efficiency to Cruciferae (especially rape) clubroot.
The bacterial strain for having higher control efficiency to clubroot being currently known is bacillus subtilis XF-1 and bacillus subtilis
Bacterium M3.Wherein, XF-1 is successfully applied in the clubroot preventing and controlling of Chinese cabbage, achieve reach as high as 85% or so it is anti-
Effect is controlled, but the application concentration of bacterium solution is very high (100 times of dilutions after culture 48h), once extension rate is promoted to 200
Times, control efficiency will sharply decline (only 69% or so).Due to, there is only 2 times of gaps, being applied between 100 times and 200 times
Operating error in the process will it is serious influence the bacterial strain biocontrol effect, cause the uncontrollable of control efficiency；In addition highly concentrated
The application of degree bacterium solution will also result in a degree of influence to diversity of soil microorganism and its surrounding enviroment.M3 bacterial strain is equally
Have the shortcomings that application bacterium is dense excessively high and control efficiency is undesirable.Currently, bacillus amyloliquefaciens (Bacillus amyloliquefaciens) in terms of Cruciferae clubroot prevention and treatment application or blank.
As shown in the embodiment of present invention, still have up to using 500 times of dilutions after strain culturing 48h of the present invention
85% or more control efficiency, even if extension rate is up to 1000 times, control efficiency is still maintained at 75% or more, significant better than existing
Some biocontrol bacterial strains.Particularly, especially prominent in terms of the control efficiency of rapeseed clubroot.
Beneficial effects of the present invention:
(1) control efficiency is significantly better than known biocontrol bacterial strain, under the requirement of environmental protection application, without joining with chemical agent
With efficient control efficiency can be reached；
(2) application concentration is low, and administration dosage is few.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
Bacillus amyloliquefaciens Bam22 tests the control efficiency of Cruciferae clubroot
Experimental subjects: rape, Chinese cabbage, broccoli.
After bacillus amyloliquefaciens Bam22 is cultivated 48h, 200 times, 500 times, 800 times, 1000 times are diluted, using pouring
Root method, when transplanting, pour 100ml to every plant of crop, pour every 7 days primary, pour again every 14 days primary.Note: the bacterium of undiluted bacterium solution is dense about
For 6-8 × 109 cfu/ml。
Severity Scaling:
0 grade: without tumour, root system development is normal for root；
1 grade: 1-30% root a little tumour, have small nodositas lump, be mutually not connected to；
2 grades: 31-60% root raw tumour, have big nodositas or spherical lump, be connected with each other；
3 grades: 61-100% root raw tumour, expand and form spindle tumor, big tumor mass extends to hypocotyl,
Plant is short and small, stops growing.
Disease index=[(diseased plant numbers at different levels × opposite value of series)/highest level × investigation total strain number] × 100.
Relative control effect=control the disease index of (control disease index-processing disease index) × 100/.
Experimental result is shown in Table 1, table 2 and table 3:
1 rapeseed clubroot of table prevents and treats result
2 cabbage clubroot of table prevents and treats result
3 broccoli clubroot of table prevents and treats result
From three above tables of data it is found that bacterial strain of the present invention preventing and treating with very outstanding for Cruciferae clubroot
Effect, meanwhile, using the bacterium solution of highly diluted multiple and when being administered with low dosage, performance control efficiency that also can be stable, tool
There is very huge practical application value.
Claims (1)
1. a plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22, which is characterized in that the high-efficiency cross-shaped flower
Section clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 was preserved in Chinese microorganism strain preservation conservator on September 6th, 2015
Meeting common micro-organisms center, deposit number are CGMCC No.11329.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510726834.5A CN105219679B (en) | 2015-11-02 | 2015-11-02 | One plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 |
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| CN201510726834.5A CN105219679B (en) | 2015-11-02 | 2015-11-02 | One plant height imitates Cruciferae clubroot biological and ecological methods to prevent plant disease, pests, and erosion bacillus amyloliquefaciens Bam22 |
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| CN105219679A CN105219679A (en) | 2016-01-06 |
| CN105219679B true CN105219679B (en) | 2019-03-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418910B (en) * | 2017-05-05 | 2020-08-25 | 昆明理工大学 | Biological agent for preventing and treating clubroot of cruciferous crops and application thereof |
| CN109042737A (en) * | 2018-07-27 | 2018-12-21 | 四川省农业科学院植物保护研究所 | A method of using biocontrol microorganisms Bam22 prevention and treatment coptis root rot and improving yield |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382781A (en) * | 2011-09-23 | 2012-03-21 | 湖北省生物农药工程研究中心 | Bacillus amyloliquefaciens for inhibiting bird rape plasmodiophora brassicae and separation and application of active substances thereof |
| CN102757917A (en) * | 2011-12-28 | 2012-10-31 | 浙江大学 | Biocontrol agent for preventing and curing bacterial wilt and club roots of plant as well as preparation method and application thereof |
| CN103724407A (en) * | 2013-12-10 | 2014-04-16 | 云南农业大学 | Anti-bacterial protein PBR1 as well as preparation method and application thereof |
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- 2015-11-02 CN CN201510726834.5A patent/CN105219679B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102382781A (en) * | 2011-09-23 | 2012-03-21 | 湖北省生物农药工程研究中心 | Bacillus amyloliquefaciens for inhibiting bird rape plasmodiophora brassicae and separation and application of active substances thereof |
| CN102757917A (en) * | 2011-12-28 | 2012-10-31 | 浙江大学 | Biocontrol agent for preventing and curing bacterial wilt and club roots of plant as well as preparation method and application thereof |
| CN103724407A (en) * | 2013-12-10 | 2014-04-16 | 云南农业大学 | Anti-bacterial protein PBR1 as well as preparation method and application thereof |
Non-Patent Citations (3)
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| 广谱拮抗植物病原真菌的芽孢杆菌菌剂研制与应用;林碧莲;《万方数据知识服务平台》;20111231;39-43 * |
| 来源于解淀粉芽胞杆菌的芸薹根肿菌抑菌活性物质的分离与鉴定;刘晓艳 等;《氨基酸和生物资源》;20140330;第36卷(第01期);摘要 * |
| 青枯病和根肿病生防细菌BS2004的发酵培养基和培养条件优化;周青;《中国生物防治学报》;20121108;第28卷(第4期);537-544 * |
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