CN108841745A - One bacillus and its preparing microorganism seed coating in application - Google Patents
One bacillus and its preparing microorganism seed coating in application Download PDFInfo
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- CN108841745A CN108841745A CN201810645249.6A CN201810645249A CN108841745A CN 108841745 A CN108841745 A CN 108841745A CN 201810645249 A CN201810645249 A CN 201810645249A CN 108841745 A CN108841745 A CN 108841745A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
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Abstract
The present invention relates to a bacillus and its application in microorganism seed coating is being prepared, is belonging to field of biotechnology.One bacillus, classification naming is bacillus megaterium (Bacillus megaterium), it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.15775, and the deposit date is on Mays 21st, 2018.The use of microorganism seed coating agent is made in above-mentioned bacillus.The preparation method of microorganism seed coating agent is：By bacillus, adhesive, film forming agent and pure water be uniformly mixed to get.Bacillus megaterium in the present invention, not only acid and alkali-resistance, moreover it is possible to reduce the pH value of culture medium and soil, and the phosphorus that cannot be utilized by plant in the soil that can degrade well, it increases soil fertility, promotes the development of root system of plant, improve plant to the absorption of nutrient, the growth of promotion plant.
Description
Technical field
The present invention relates to one plant of bacterium and its applications, and in particular to a bacillus and its is preparing microbial species attached bag
Application in clothing, belongs to field of biotechnology.
Background technique
Phosphorus-solubilizing bacteria (Phosphate-solubilizing microorganism, PSM) is a kind of special micro- in soil
Biological group, one kind that the invalid phosphorus that can be difficult to plant to be absorbed and utilized is converted into the absorbable effective P form utilized are special
The flora of microbial function plays key effect in natural and agroecological phosphorus element biology global chemical recycle.Phosphorus-solubilizing bacteria
Secretions from plant roots can be influenced, plant is promoted by secreting various enzymes or organic acid come the indissoluble state phosphorus in activating soil
Absorption of the root system to nutrients such as K, N, Ca, Mg, Fe, Zn, promotes the growth and development of plant, influences the group of rhizosphere microorganism
Structure.
Bacillus is a quasi-microorganism important in Soluble phosphorus microorganism, has that resistance is strong, nutrition is simple, reproduction speed
Fastly, the advantages such as living bacteria count amount is high, performance is stable, Storage period is long.And certain strains also have diseases prevention, growth-promoting, Soluble phosphorus, molten
Potassium, nitrogen fixation have important practical significance for developing eco-agriculture.
Currently, phosphorus-solubilizing bacteria separation screening gained mostly from soil or root soil, mainly as micro- in the form of bacterial manure
Bacteria agent application there have been no the report that phosphorus-solubilizing bacteria and seed coat agent are used in compounding.
Summary of the invention
The first purpose of this invention is to provide a bacillus.
Second object of the present invention is to provide the bacillus in microorganism seed coating and soil phosphorus decomposing
Application.
In order to achieve the above first purpose, the technical solution adopted in the present invention is as follows：
One bacillus, classification naming are bacillus megaterium (Bacillusmegaterium), and it is micro- to be preserved in China
Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.15775, and the deposit date is 2018 5
The moon 21.
In the Anhui Suzhou City Xiao County (34 ° 11 ' 53.10 ", 116 ° 58 ' 50.53 ") of acquisition on July 5th, 2017 to day
Isolated bacillus MN213230 according to the present invention in certain herbaceous plants with big flowers root soil；
Using dilution-plate method and spread plate by sample coated plate in carrying out separation screening on organic phosphorus solid medium.
Utilize primer 2 7F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-
TACGGYTACCTTGTTACGACTT-3') bacterial strain MN213230 is expanded and is sequenced, obtaining sequence length is 1405bp,
The 16S rDNA sequence of bacterial strain MN213230 is as shown in sequence table.By BLAST by sequence in sequence and GenBank database into
Row comparison, the similitude with Bacillus megaterium are 100, are identified as bacillus megaterium Bacillus
megaterium。
To reach above-mentioned second purpose, the technical solution adopted in the present invention is as follows：
Application of the above-mentioned bacillus in soil phosphorus decomposing.
The use of microorganism seed coating agent is made in above-mentioned bacillus.
Further, the preparation method of microorganism seed coating agent is：
By bacillus, adhesive, film forming agent and pure water be uniformly mixed to get.
Further, the quantity of viable bacteria is 1 × 10 in microorganism seed coating agent4Cfu/mL~1 × 108cfu/mL。
Further, described adhesive is polyvinylpyrrolidone.
Further, the film forming agent is polyethylene glycol.
Beneficial effect：The bacillus is applied to microorganism seed coating by the present invention, and is used for Soluble phosphorus.In the present invention
Bacillus megaterium, not only acid and alkali-resistance, moreover it is possible to reduce the pH value of culture medium and soil, and in the soil that can degrade well not
The phosphorus that can be utilized by plant, increases soil fertility, and promotes the development of root system of plant, improves plant and plants to absorption, the promotion of nutrient
The growth of object.
Detailed description of the invention
Fig. 1 is the Gram's staining figure of bacillus of the invention
Fig. 2 is the phosphorus decomposing circle of bacillus of the invention on organic phosphorus solid medium repeating effect figure three times.
Fig. 3 is the aspect graph of bacillus of the invention on LB solid medium.
Fig. 4 is bacillus of the invention applied to, to the growth effect figure of corn, wherein A is control group after corn coating
(CK), B is experimental group.
Specific embodiment：
Detailed description of specific embodiments of the present invention with reference to the accompanying drawing.
1, separate and identify part
Bacillus megaterium (Bacillus megaterium) MN213230 of the invention, which is located away from, to be adopted on July 5th, 2017
The sunflower root soil of the Anhui Suzhou City Xiao County (34 ° 11 ' 53.10 ", 116 ° 58 ' 50.53 ") of collection.
Using dilution-plate method and spread plate by sample coated plate in organic phosphorus solid medium (glucose 10g,
(NH4)2SO40.5g, yeast extract 0.5g, NaCl 0.3g, KCl 0.3g, FeSO40.03g, MnSO40.03g, Ca3
(PO4)21g, MgSO40.3g, lecithin 0.2g, agar 15-20g, water 1L, pH 7) on carry out separation screening acquisition, microscope
It is as shown in Figure 1 to observe form.
Separation method is：5g samples-soil is weighed in 50mL sterile centrifugation tube, 45mL sterile water, vortex oscillation 2 is added
~3min, is dispersed in soil particle in sterile water, obtains 10 times (10 of dilution-1) Soil Slurry, be placed at room temperature for
25min.5mL suspension (to mix before absorption) is drawn in the centrifuge tube equipped with 45mL aqua sterilisa, obtains dilution 10-2Suspension
Liquid, 10 will obtained-2Soil Slurry sufficiently shake up, as above continue to be diluted to 10-3-10-5, take its 10-3, 10-4, 10-5Into
The coating of row plate, each gradient are coated with three in parallel, and 28 DEG C, 2-4d, whether observation plate occurs phosphorus decomposing circle, have picked out solution
The bacterial strain of phosphorus circle saves, and carries out the work of next step secondary screening.
Bacterial strain MN213230 has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (referred to as
CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), deposit number CGMCC
No.15775, the deposit date is on Mays 21st, 2018.With following Microbiological Characteristics：In LB solid medium (tryptone
10g, yeast powder 5g, sodium chloride 10g, agar 15-20g, water 1L, pH 7) grow for 24 hours on plate after, bacterium colony is round, milky,
Surfacing moistens (see Fig. 3).
Utilize primer 2 7F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-
TACGGYTACCTTGTTACGACTT-3') bacterial strain MN213230 is expanded and is sequenced, obtaining sequence length is 1405bp,
The 16S rDNA sequence of bacterial strain MN213230 is as shown in sequence table.By BLAST by sequence in sequence and GenBank database into
Row comparison, the similitude with Bacillus megaterium are 100, are identified as bacillus megaterium Bacillus
megaterium。
2, the effect of solubilizing phosphate measurement of bacterial strain MN213230
Test solid plate measurement
Bacterial strain MN213230, which is inoculated in, to be solved on organic phosphorus solid plate, and each three repetitions, observe phosphorus decomposing by 28 DEG C after 5d
Circle, and phosphorus decomposing circle (D) and colony diameter (d) have been recorded, D/d value is calculated, colony diameter of the bacterial strain in solid medium is detected
And the solvability to solid plate insoluble phosphorus.The colony diameter of bacterial strain MN213230 is 9.3mm, and phosphorus decomposing loop diameter is
The ratio of 14mm, D/d are 1.5, as a result as shown in Figure 2.
Test two soil water experiments
Inoculation liquid：Activated phosphate solubilizing bacteria bacillus MN213230 is inoculated into the LB liquid medium equipped with 100mL
In the 250mL triangular flask of (tryptone 10g, yeast powder 5g, sodium chloride 10g, water 1L, pH 7), 30 DEG C of 180r/min oscillation trainings
It supports and is used as seed liquor for 24 hours, then seed liquor is diluted to living bacteria count about 1 × 108Cfu/mL obtains inoculation liquid.
0.5% calcium phosphate is added in soil in mass ratio, after mixing, respectively fills 100g with the triangular flask of 250mL and is mixed with
The soil of calcium phosphate, then add the water seal of 20mL pure water good respectively, using moist hear heat test, 121 DEG C of sterilizing 60min.Experimental group is every
Inoculation liquid 5mL is added in bottle, and the LB liquid medium of 5mL sterilizing, 3 repetitions of each processing, after inoculation are then added in control group (CK)
It is placed in 28 DEG C of incubators, soil available phosphorus is measured by sampling in 42d.It detects available phosphorus content and (uses NY/T 1121.7-2014
Standard test soil available phosphorus).Specific data are as follows：
| CK | Experimental group | |
| Repeat 1 | 16.92 | 28.98 |
| Repeat 2 | 18.60 | 28.29 |
| Repeat 3 | 15.24 | 28.29 |
| Mean value | 16.91 | 28.30 |
As the result is shown：The average value of the available phosphorus content of control group is 16.91mg/kg, the available phosphorus content of experimental group
Average value is 28.30mg/kg, and experimental group increases 67.24% available phosphorus content than control group.
3, the resistance to acid and alkali effect measuring of bacterial strain MN213230
The configuration of pH value gradient media：It is basic culture medium with LB liquid medium, thereto plus NaOH or HCl solution tune
Its pH value is saved, pH value is respectively a series of 2.0,3.0,4.0,5.0,6.0,7.0,8.0,9.0,10.0,11.0,12.0 LB training
Base is supported, cooling is spare after 121 DEG C of sterilizing 25min.
Phosphorus-solubilizing bacteria MN213230 is inoculated in the LB culture medium of 100mL difference pH value gradient, inoculation bacterial concentration is
1083 repetitions, after inoculation, the 180r/ in 28 DEG C of constant temperature oscillator are arranged in cfu/mL, inoculum concentration 100uL, each gradient
Min vibrates 48h, checks the growing state of bacterial strain.
As the result is shown：Bacterial strain MN213230 has certain acidproof alkali ability, can grow between 5-10 in pH value.
4, bacillus MN213230 is promoting the application in plant root system development
Seed treatment：By bacterium number about 1 × 108The bacterium solution of the MN213230 of cfu/mL and a certain amount of adhesive (polyethylene
Pyrrolidones), film forming agent (polyethylene glycol) be configured to simple seed coat agent (10% bacterium solution, 5% film forming agent, 2% bonding
Agent, others are pure water), then by the simple seed coat agent and size, the much the same corn seed of quality with 1：30 weight ratio
Mixing coating is carried out, enables bacterial strain uniform adsorption on seed, there is 1 × 10 on seed4Cfu/mL~1 × 108The bacterium of cfu/mL
Number.
By the sterilized soil (soil crosses 10 meshes, and 121 DEG C, sterilize 1h, adds 0.5% tricalcium phosphate) of 3Kg mounted in 32 holes
In seedlings nursing plate.To there is the seed of bacterium as experimental group, using sterile seed as control group (CK), each processing 16 repeats basin
Plant experiment, Routine Management.It is harvested after growth 20d, measures single plant plant height, stem length, root weight and dry weight；And soil sample is collected, into
The pH value (using NY/T 1121.2-2006 standard test soil pH value) of row soil and available phosphorus content test.
Potted plant experiment result：From data it is found that the Corn Biomass of experimental group is significantly higher than the jade of control group (CK)
Rice (comparative diagram of the potted plant experiment of corn such as Fig. 4), especially root system, the root system of experimental group on control (CK) group fresh weight than increasing
23.39%, dry weight increases 15.04%.The soil available phosphorus of experimental group increases 4.4mg/kg than the available phosphorus of control group,
PH value has dropped 0.52, and detailed results are shown in Table 1, table 2.In Tables 1 and 2, difference is aobvious between the different expression processing of a, b letter in table
It writes, letter is identical to indicate no difference.
1 bacterial strain MN213230 of table and corn seed dressing influence the biomass of seedling growth of corn
The variation of available phosphorus and soil pH value in 2 soil of table
It follows that bacterial strain MN213230 can promote the development of root system of plant well, the nutrient absorption of plant is improved, is promoted
Into plant growth；Soil pH value is reduced, soil inavailable phosphorus of degrading improves soil available phosphorus content, increases soil fertility.Bacterial strain
MN213230, which is coated corn, see the table below the influence of the biomass of seedling growth of corn.
Embodiments of the present invention are elaborated above.But present invention is not limited to the embodiments described above,
Technical field those of ordinary skill within the scope of knowledge, can also do without departing from the purpose of the present invention
Various change out.
Sequence table
<110>Guangzhou bacterium colony Biotechnology Co., Ltd
<120>One bacillus and its preparing microorganism seed coating in application
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1405
<212> DNA
<213>Bacillus megaterium (Bacillus megaterium)
<400> 1
actgattaga agcttgcttc tatgacgtta gcggcggacg ggtgagtaac acgtgggcaa 60
cctgcctgta agactgggat aacttcggga aaccgaagct aataccggat aggatcttct 120
ccttcatggg agatgattga aagatggttt cggctatcac ttacagatgg gcccgcggtg 180
cattagctag ttggtgaggt aacggctcac caaggcaacg atgcatagcc gacctgagag 240
ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg 300
gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga tgaaggcttt 360
cgggtcgtaa aactctgttg ttagggaaga acaagtacga gagtaactgc tcgtaccttg 420
acggtaccta accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg 480
tggcaagcgt tatccggaat tattgggcgt aaagcgcgcg caggcggttt cttaagtctg 540
atgtgaaagc ccacggctca accgtggagg gtcattggaa actggggaac ttgagtgcag 600
aagagaaaag cggaattcca cgtgtagcgg tgaaatgcgt agagatgtgg aggaacacca 660
gtggcgaagg cggctttttg gtctgtaact gacgctgagg cgcgaaagcg tggggagcaa 720
acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttagagggt 780
ttccgccctt tagtgctgca gctaacgcat taagcactcc gcctggggag tacggtcgca 840
agactgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat 900
tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ctgacaactc tagagataga 960
gcgttcccct tcgggggaca gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1020
gagatgttgg gttaagtccc gcaacgagcg caacccttga tcttagttgc cagcatttag 1080
ttgggcactc taaggtgact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1140
catcatgccc cttatgacct gggctacaca cgtgctacaa tggatggtac aaagggctgc 1200
aagaccgcga ggtcaagcca atcccataaa accattctca gttcggattg taggctgcaa 1260
ctcgcctaca tgaagctgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg 1320
ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1380
tggagtaacc gtaaggagct agccg 1405
Claims (7)
1. a bacillus, classification naming is bacillus megaterium (Bacillus megaterium), is preserved in the micro- life of China
Object culture presevation administration committee common micro-organisms center, deposit number are CGMCC No.15775, and the deposit date is in Mays, 2018
21 days.
2. application of the bacillus described in claim 1 in soil phosphorus decomposing.
3. the use of microorganism seed coating agent is made in bacillus described in claim 1.
4. application according to claim 3, which is characterized in that the preparation method of microorganism seed coating agent is：
By bacillus, adhesive, film forming agent and pure water be uniformly mixed to get.
5. application according to claim 4, which is characterized in that in microorganism seed coating agent the quantity of viable bacteria be 1 ×
104Cfu/mL~1 × 108cfu/mL。
6. application according to claim 4, which is characterized in that described adhesive is polyvinylpyrrolidone.
7. application according to claim 4, which is characterized in that the film forming agent is polyethylene glycol.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699234A (en) * | 2018-08-30 | 2019-05-03 | 四川省农业科学院植物保护研究所 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
| CN109757488A (en) * | 2019-02-20 | 2019-05-17 | 广东省农业科学院植物保护研究所 | A kind of microorganism seed pill pelletizing coating composition |
| CN111484952A (en) * | 2019-01-28 | 2020-08-04 | 福建省农业科学院农业生物资源研究所 | Bacillus for dissolving phosphorus and fixing nitrogen and application thereof |
| CN117586914A (en) * | 2023-11-22 | 2024-02-23 | 上海交通大学 | Salt-tolerant phosphate-dissolving nitrogen-fixing bacterium for soil and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164288A (en) * | 2017-07-14 | 2017-09-15 | 安徽农业大学 | A kind of compound Soluble phosphorus microbial inoculum and its preparation method and application |
-
2018
- 2018-06-21 CN CN201810645249.6A patent/CN108841745A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164288A (en) * | 2017-07-14 | 2017-09-15 | 安徽农业大学 | A kind of compound Soluble phosphorus microbial inoculum and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 李海云等: "土壤溶磷微生物研究进展", 《生物学通报》 * |
| 王琰等: "解磷芽孢杆菌的筛选鉴定及其对玉米促生机理的研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109699234A (en) * | 2018-08-30 | 2019-05-03 | 四川省农业科学院植物保护研究所 | A kind of seed-coating method and capsuled seed preventing rapeseed clubroot |
| CN111484952A (en) * | 2019-01-28 | 2020-08-04 | 福建省农业科学院农业生物资源研究所 | Bacillus for dissolving phosphorus and fixing nitrogen and application thereof |
| CN109757488A (en) * | 2019-02-20 | 2019-05-17 | 广东省农业科学院植物保护研究所 | A kind of microorganism seed pill pelletizing coating composition |
| CN117586914A (en) * | 2023-11-22 | 2024-02-23 | 上海交通大学 | Salt-tolerant phosphate-dissolving nitrogen-fixing bacterium for soil and application thereof |
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Application publication date: 20181120 |