CN103215209A - Bacillus amyloliquefaciens and application thereof - Google Patents

Bacillus amyloliquefaciens and application thereof Download PDF

Info

Publication number
CN103215209A
CN103215209A CN201310145532XA CN201310145532A CN103215209A CN 103215209 A CN103215209 A CN 103215209A CN 201310145532X A CN201310145532X A CN 201310145532XA CN 201310145532 A CN201310145532 A CN 201310145532A CN 103215209 A CN103215209 A CN 103215209A
Authority
CN
China
Prior art keywords
bacillus amyloliquefaciens
rcd
biological control
grape
bacterial strain
Prior art date
Application number
CN201310145532XA
Other languages
Chinese (zh)
Other versions
CN103215209B (en
Inventor
李鹏
牛赡光
王清海
李泉涌
刘幸红
李红梅
Original Assignee
牛赡光
李鹏
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 牛赡光, 李鹏 filed Critical 牛赡光
Priority to CN201310145532.XA priority Critical patent/CN103215209B/en
Publication of CN103215209A publication Critical patent/CN103215209A/en
Application granted granted Critical
Publication of CN103215209B publication Critical patent/CN103215209B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The invention discloses bacillus amyloliquefaciens and application thereof, and belongs to the field of biological control of plant diseases. The strain is bacillus amyloliquefaciens RCD-1, and the collection number is CGMCC No.7502. The invention further discloses a biological control preparation containing the strain and a preparation method of the biological control preparation. The bacillus amyloliquefaciens strain provided by the invention has the characteristics of high growth speed, high spore yield, wide effect spectrum and strong stress resistance, and can be fast colonized in quantity at the plant roots, so good application prospect is achieved. The biological control preparation prepared from the bacillus amyloliquefaciens not only can effectively control the soil-borne disease of the plant, but also can increase the crop output, and is a biological control preparation with great application prospect. The microbial preparation can serve as biopesticide or biological fertilizer for controlling various soil-borne plant diseases, especially grape white rot.

Description

One bacillus amyloliquefaciens and application thereof
Technical field
The invention belongs to biological control Plant diseases field.Particularly, the present invention relates to bacillus amyloliquefaciens that a strain can the high-efficiency prevention and control Plant diseases and uses thereof, and the biological control preparation that adopts this bacillus amyloliquefaciens preparation.
Background technology
Grape is a defoliation liana, and the title of " god of fruit " is arranged, and is one of the most ancient in the world plant.Grape originates in Europe, West Asia and north African one band.In the main place of production of China is ground such as Xinjiang, Gansu, Shanxi, Hebei, Shandong.It is directly edible that grape both can be used as fruit, also can be as making grape wine.Grape is edible to have the antiviral effects such as bacterium, cancer-resisting, reduction hydrochloric acid in gastric juice cholagogic, atherosclerosis, help and excited cerebral nerve, inducing diuresis to remove edema be antiabortive of killing; But the grape wine invigorating qi and benefiting blood of brewageing, Yin-nourishing and body fluid promoting, strengthening the muscles and bones, tonneau urine.Cure mainly deficient qi and blood, chronic cough of deficiency lung, the hepatic and renal YIN deficiency, palpitation and night sweat, the waist and leg ache, muscles and bones is unable, rheumatic arthralgia, face limb edema, illnesss such as dysuria.Therefore the grape market requirement is vigorous.But because the harm of grape disease has had a strong impact on the sound development of grape industry.Fruit white rot of grape (Coniella diplodiella) is one of heavier disease of viny region harm, and the annual production loss that is caused by this disease reaches 10%~30%, and serious plot reaches more than 50%.This disease pathogen bacterium is mainly infected fruit, young stem and inflorescence etc., and is wherein serious with fruit harm, causes fruit rot and shattering.In a single day disease spreads, bigger to output, quality, benefit influence, causes the underproduction of many orchards even faces the danger in the garden of ruining.At the control of this disease,, but tend to bring problems such as human cost is too high, medication blindness, super concentration medication mainly with bagging or chemical prevention.Therefore the biological control technology based on bio-pharmaceutical is the inexorable trend of fruit white rot of grape control.
Genus bacillus is a kind of saprophytic bacteria that is distributed widely in the various different living environments, is the general name that the aerobic double anaerobic of a group produces the gemma Gram-positive bacillus, gives birth to heat-resisting degeneration-resistant gemma in can producing.Gemma not only has resistance to heat, also ultraviolet ray, electromagnetic radiation and some pharmaceutical chemicalss is had very strong resistance, and it can stand various poor environments.Owing to have so strong resistance, make it help the production and the formulation processing of biocontrol fungicide, also be beneficial to its survival in environment, grow and breeding surely.Because their growths are fast, nutrition simple, can form the gemma with strong resistance ability, advantage such as more non-genus bacillus living bacteria count amount height, stable performance in product development and getting most of the attention.Genus bacillus is the important component part of Plant diseases biological and ecological methods to prevent plant disease, pests, and erosion microorganism, can be used for preventing and treating plurality of plant diseases.Genus bacillus mainly contains as the mechanism of biocontrol microorganisms controlling plant diseases: antibiosis, nutrient competition, competition for space, hyperparasitism and inducing plant produce mechanism such as resistance, and the Biocontrol Mechanism of genus bacillus mainly is to suppress harmful pathogen growth or kill pathogenic bacteria to produce antagonistic substance.These materials overwhelming majority is a peptide antibiotics, has efficient, low residue and advantages of environment protection.
Summary of the invention
The purpose of this invention is to provide a kind of new genus bacillus that is used for the high-efficiency prevention and control plurality of plant diseases---bacillus amyloliquefaciens RCD-1.This bacterial strain has fast growth, sporulation quantity is big, action spectrum is wide, strong stress resistance, characteristics such as can grow fast in a large number surely at plant rhizosphere, therefore has a good application prospect.
Bacterial strain provided by the present invention is bacillus amyloliquefaciens (Bacillus amyloliquefaciens) RCD-1, be from the soil on coastal beach ground, backlands, the Weihe River, Weifang City, Shandong Province district, to separate to obtain, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 22nd, 2013 and (be called for short CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica), preserving number is CGMCC No.7502.It has following biological characteristics: colonial morphology is circular or irregular shape on beef extract-peptone nutrient agar or LB substratum, milky white yellow partially, surface irregularity plays fold, on the beef extract-peptone nutrient agar, cultivate two days later down for 28 ℃, microscopy, somatic cells are shaft-like, can move.Through gramstaining be positive (being contrast with intestinal bacteria).After spore staining, observe gemma ovalize or column.
Cultural method or the propagation method of bacillus amyloliquefaciens of the present invention (Bacillus amyloliquefaciens) bacterial strain RCD-1 comprise:
(1) common cultivation is preserved and is adopted beef extract-peptone agar (NA) substratum, and filling a prescription is Tryptones 10g, extractum carnis 3g, and sodium-chlor 5g, agar 15-20g, distilled water 1000mL, pH transfers to 7.0-7.2;
(2) laboratory fluids is cultivated and is adopted the beef extract-peptone liquid nutrient medium, and filling a prescription is Tryptones 10g, extractum carnis 3g, and sodium-chlor 5g, distilled water 1000mL, pH transfers to 7.0-7.2;
(3) bulk fermentation is cultivated prescription: analysis for soybean powder 2.5%, Semen Maydis powder 3.5%, NaCl0.1%, CaCO 30.2%, all the other are water.
The present invention also provides a kind of biological control preparation that is used to prevent and treat plant soil-borne diseases, and described biological control preparation comprises described bacillus amyloliquefaciens bacterial strain RCD-1.
And the present invention also provides a kind of biological control method that is used to prevent and treat plant soil-borne diseases, and described biological control method comprises to the plant with soil-borne disease uses above-mentioned bacillus amyloliquefaciens bacterial strain RCD-1 or above-mentioned biological control preparation.
The present invention also provides above-mentioned bacillus amyloliquefaciens bacterium or the purposes of above-mentioned biological control preparation in the control plant soil-borne diseases.
With regard to above-mentioned biological control preparation, biological control method and purposes, described Plant diseases can be selected from comprise fruit white rot of grape, cucumber anthracnose, walnut anthrax, apple anthracnose, bitter rot or anthracnose of grape, watermelon anthrax, winter jujube black spot, Cure of Coryneum kunzei, pomegranate dry rot etc. one or more.
In a specific embodiments of the present invention, described preparation method may further comprise the steps:
(1) spore of bacillus amyloliquefaciens bacterial strain RCD-1 is transplanted in the beef extract-peptone liquid nutrient medium, 28-30 ℃ of shaking table shaking culture 2-3d obtains seed liquor;
(2) in 3.0% ratio (volume ratio) seed liquor is inoculated in the bulk fermentation substratum, culture temperature 28-30 ℃, incubation time 30h, ventilating ratio 1:0.9.Obtain fermented liquid after fermentation is finished, fermented liquid bacterium number can reach 10,000,000,000/g.
(3) bacillus amyloliquefaciens fermented liquid and matrix (be selected from the peat composed of rotten mosses, micro mist lime carbonate and the wilkinite one or more) are mixed, drying, the bacterium number can reach 50,000,000,000/g after drying.The mass ratio of described fermented liquid and fermented liquid matrix is 1:1.
Experiment shows, bacillus amyloliquefaciens bacterial strain of the present invention has fast growth, produce that the gemma amount is big, action spectrum wide, strong stress resistance, characteristics such as can grow fast in a large number surely at plant rhizosphere, therefore has a good application prospect.By the biological control preparation of this bacillus amyloliquefaciens preparation, not only can prevent and treat plant soil-borne diseases efficiently, can also effectively improve crop yield, be a kind of biological control preparation that has application prospect.This microbial preparation can be used as biological pesticide or bio-feritlizer, prevent and treat multiple soil and pass Plant diseases, comprise fruit white rot of grape, cucumber anthracnose, walnut anthrax, apple anthracnose, bitter rot or anthracnose of grape, watermelon anthrax, winter jujube black spot, Cure of Coryneum kunzei, pomegranate dry rot etc. one or more.
Embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall within the scope of protection of the present invention the details of technical solution of the present invention and form.
Embodiment 1
1, the isolation and purification of bacillus amyloliquefaciens (B.amyloliquefaciens) bacterial strain RCD-1
Bacillus amyloliquefaciens of the present invention (B.amyloliquefaciens) RCD-1 adopts dilution-plate method to separate acquisition with plate streak from soil, and separation method is:
(1) separation of bacillus: the collection of soil sample, on coastal beach ground, backlands, the Weihe River, Weifang City, Shandong Province district, adopt 5 point samplings, gather around the plot respectively and the soil of the central degree of depth in the 10-20cm scope an amount of, the equivalent mixing.Indicate collecting location, time and collection people.Take by weighing the 1g soil sample in the 100mL sterilized water, place 30 ℃ of shaking table 150rpm concussion 10min, place 60 ℃ of water-baths to hatch 30min then, get 100 μ L10 -2, 10 -3, 10 -4Diluent is coated on the beef-protein medium flat board, each three of gradient coating is parallel, the microbial bacteria of the different shape on picking beef-protein medium behind 30 ℃ of cultivation 2d falls within on the beef-protein medium flat board rules, and regularly observes the colony growth situation.Adopt plate streak then, the purifying Bacillus strain, numbering is preserved respectively.
⑵ the screening of the efficient antagonism genus bacillus of fruit white rot of grape
1. primary dcreening operation: adopt the face-off culture method, preparation PDA flat board is bought the bacterium cake that cut-off directly is 5mm with punch tool at fruit white rot of grape bacterium mycelia edge, is implanted in dull and stereotyped central authorities, and 2cm place inoculates the genus bacillus strain all around, puts 27 ℃ of biochemical incubators cultivations.Day by day observe the restraining effect of genus bacillus to pathogenic bacteria.
2. sieve again: the Bacillus strain with efficient antagonistic activity that will screen carries out multiple sieve, mainly is through temperature tolerance, resistance to acids and bases, drug-resistant test, screens patience Bacillus strain preferably, carries out potted plant control test and field test.
The inventor obtains bacillus amyloliquefaciens (B.amyloliquefaciens) the bacterial strain RCD-1 that a strain can the high-efficiency prevention and control plurality of plant diseases by a large amount of screening operations.Experiment showed, that this bacillus amyloliquefaciens demonstrates prevention effect very efficiently preventing and treating fruit white rot of grape, makes farm crop significantly increase production.Thereby bacillus amyloliquefaciens of the present invention is the new bacterial strain of bacillus amyloliquefaciens with wide application prospect, can be used to prepare the biological control preparation of preventing and treating plurality of plant diseases.
2, identification of strains
(1) Microbiological Characteristics: colonial morphology is circular or irregular shape on beef extract-peptone nutrient agar or LB substratum, and milky white yellow partially, surface irregularity plays fold, on the beef extract-peptone nutrient agar, cultivate two days later microscopy down for 28 ℃, it is shaft-like that somatic cells is, and can move.Through gramstaining be positive (being contrast with intestinal bacteria).After spore staining, observe gemma ovalize or column.
(2) molecular biological characteristic
The strain 16s? RDNA gene sequence determination results are as follows(SEQ-1)CGCCTGCCTATACTGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTCTGAACCGCATGGTTCAGACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTGCCGTTCAAATAGGGCGGCACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTC GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTATGAGCCAGCCGCCGAAGGTG
Embodiment 2
1, bacillus amyloliquefaciens bacterial strain RCD-1 fermenting process
The beef extract-peptone liquid nutrient medium, filling a prescription is: Tryptones 10g, extractum carnis 3g, sodium-chlor 5g, distilled water 1000mL, pH transfers to 7.0-7.2.
A large amount of liquid fermentation medium prescriptions (quality percentage composition): analysis for soybean powder 2.5%, Semen Maydis powder 3.5%, NaCl0.1%, CaCO 30.2%, all the other are water.
The a large amount of solid fermentation processes of bacillus amyloliquefaciens bacterial strain RCD-1:
(1) spore of bacillus amyloliquefaciens bacterial strain RCD-1 is transplanted in the beef extract-peptone liquid nutrient medium, 28-30 ℃ of shaking table shaking culture 2-3d obtains seed liquor;
(2) in 3.0% ratio (volume ratio) seed liquor is inoculated in the bulk fermentation substratum, culture temperature 28-30 ℃, incubation time 30h, ventilating ratio 1:0.9.Obtain fermented liquid after fermentation is finished, fermented liquid bacterium number can reach 10,000,000,000/g.
(3) bacillus amyloliquefaciens fermented liquid and matrix (be selected from the peat composed of rotten mosses, micro mist lime carbonate and the wilkinite one or more) are mixed, drying, fermented liquid bacterium number can reach 50,000,000,000/g after drying.The mass ratio of described fermented liquid and fermented liquid matrix is 1:1.
Embodiment 3 bacillus amyloliquefaciens bacterial strain RCD-1 verify fruit white rot of grape bacterium room effect
1. experimental technique
Adopt dull and stereotyped face-off culture method, will move to dull and stereotyped central authorities for the fruit white rot of grape bacterium bacterium cake (9mm) of examination, the bacillus amyloliquefaciens of 2cm place inoculation all around RCD-1 bacterial strain is put 27 ℃ of biochemical incubators and is cultivated, and each is handled and repeats 3 times.When mycelia to be contrasted is covered with flat board, adopt the right-angled intersection method to measure colony diameter, antibacterial band diameter, calculating mean value and relative inhibition.
2. result
As can be seen from Table 1, bacillus amyloliquefaciens bacterial strain RCD-1 all has stronger restraining effect to fruit white rot of grape bacterium mycelial growth, and antibacterial band diameter is 15.16mm, and mycelial growth inhibition rate is 83.17%.
Table 1. bacillus amyloliquefaciens bacterial strain RCD-1 measures the indoor bacteriostatic activity of fruit white rot of grape bacterium
Embodiment 4 bacillus amyloliquefaciens bacterial strain RCD-1 wettable powders are to fruit white rot of grape field compliance test result
Present embodiment provides the related experiment of bacillus amyloliquefaciens bacterial strain RCD-1 wettable powder at fruit white rot of grape.
1.1 reagent agent
Bacillus amyloliquefaciens RCD-1 wettable powder; 50% thiram wettable powder (commercially available).
The bacterium powder 2% of bacillus amyloliquefaciens RCD-1 wettable powder agent prescription (weight ratio): embodiment 2, CMC0.5% draws back powder 3%, sodium laurylsulfonate 8%, glucose 2%, white carbon black 1%, all the other are attapulgite.The bacterium work of this wettable powder is 1,000,000,000/gram.
1.2 for studying thing and controlling object:
For studying thing is grape, and kind is a cigarette 73
Controlling object: white rot
1.3 situation experimental field
Experimental field be located in the wine-growing garden, Yantai City, Shandong Province, carried out woodland respectively at 2011 in wine-growing garden, Yantai City and prevent and treat the fruit white rot of grape test.Soil is sand ground.Grapevine is life in 5 years, cultivates from posture.Tree vigo(u)r is medium on the weak side, and disease takes place serious.All experimental plot cultivation conditions and control measures unanimity
1.4 test design and arrangement
This test design 300 times, 500 times, 700 times of bacillus amyloliquefaciens (B.amyloliquefaciens) RCD-1 wettable powders, 700 times of liquid of 50% thiram water dispersible granules and not dispenser clear water compare totally 5 processing, each sub-district 24m 2, repeat totally 20 sub-districts, each sub-district random alignment 4 times.June 20 (premorbid) in 2011, later on every two all dispensers 1 time, totally 6 times.
1.5 pilot survey and method of calculation
1.5.1 efficacy survey, control time and number of times
1 drug effect of investigation is investigated all fruit ears of each sub-district before the fruit harvesting, and the total fruit of record number, sick fruit number calculate prevention effect with diseased fruit rate, carry out data analysis with DPS software.
1.5.2 drug effect method of calculation
Drug effect is calculated by formula (1), (2):
2. result
2.1 reagent agent is to the prevention effect of fruit white rot of grape
Table 2 result shows, bacillus amyloliquefaciens (B.amyloliquefaciens) RCD-1 wettable powder has the better prevention effect to fruit white rot of grape, wherein 300 times, 500 times prevention effect of bacillus amyloliquefaciens (B.amyloliquefaciens) RCD-1 wettable powder are best, prevention effect reaches 80.47%, 77.79% respectively, significant difference on P<0.05 level is apparently higher than the prevention effect of 700 times and 50% thiram.
Table 2 bacillus amyloliquefaciens RCD-1 wettable powder is prevented and treated fruit white rot of grape field control effectiveness test result
Annotate: in the table after the data different lowercases represent significant difference behind the Duncan multiple comparisons (P<0.05).
3. brief summary
From grape diseased fruit rate and prevention effect, bacillus amyloliquefaciens (B.amyloliquefaciens) the RCD-1 wettable powder of 1,000,000,000/gram has the better prevention effect to fruit white rot of grape, prevention effect can reach more than 77.79% after diluting 300 times, 500 times, significantly better than contrast medicament, significant difference.

Claims (8)

1. bacillus amyloliquefaciens (Bacillus amyloliquefaciens) RCD-1, the deposit number of described bacterial strain is CGMCC No.7502.
2. the described bacillus amyloliquefaciens of claim 1 (Bacillus amyloliquefaciens) bacterial strain RCD-1 is in the purposes of control in the plant soil-borne diseases.
3. purposes as claimed in claim 2, it is characterized in that described plant soil-borne diseases is one or more in fruit white rot of grape, cucumber anthracnose, walnut anthrax, apple anthracnose, bitter rot or anthracnose of grape, watermelon anthrax, winter jujube black spot, Cure of Coryneum kunzei, the pomegranate dry rot.
4. a biological control preparation comprises the described bacillus amyloliquefaciens of claim 1 (Bacillus amyloliquefaciens) bacterial strain RCD-1.
5. a method for preparing the described biological control preparation of claim 4 is characterized in that, may further comprise the steps:
(1) spore of bacillus amyloliquefaciens bacterial strain RCD-1 is transplanted in the beef extract-peptone liquid nutrient medium, 28-30 ℃ of shaking table shaking culture 2-3d obtains seed liquor; Described beef extract-peptone liquid nutrient medium is: Tryptones 10g, and extractum carnis 3g, sodium-chlor 5g, distilled water 1000mL, pH transfers to 7.0-7.2;
(2) 3.0% ratio is inoculated into the seed liquor of step (1) in the bulk fermentation substratum by volume, and culture temperature 28-30 ℃, incubation time 30h, ventilating ratio 1:0.9; After finishing, fermentation obtains fermented liquid; Described bulk fermentation substratum is: analysis for soybean powder 2.5%, and Semen Maydis powder 3.5%, NaCl 0.1%, CaCO 30.2%, all the other are water;
(3) fermented liquid and the matrix with step (2) mixes drying; The mass ratio of described fermented liquid and matrix is 1:1; Described matrix is selected from one or more in the peat composed of rotten mosses, micro mist lime carbonate and the wilkinite.
6. the described biological control preparation of claim 4 is in the purposes of control in the plant soil-borne diseases.
7. purposes as claimed in claim 6, it is characterized in that described plant soil-borne diseases is one or more in fruit white rot of grape, cucumber anthracnose, walnut anthrax, apple anthracnose, bitter rot or anthracnose of grape, watermelon anthrax, winter jujube black spot, Cure of Coryneum kunzei, the pomegranate dry rot.
8. the biological control method of a plant soil-borne diseases is characterized in that, uses bacillus amyloliquefaciens (Bacillus amyloliquefaciens) the bacterial strain RCD-1 of claim 1 or the biological control preparation of claim 4 to the plant with soil-borne disease.
CN201310145532.XA 2013-04-24 2013-04-24 Bacillus amyloliquefaciens and application thereof Expired - Fee Related CN103215209B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310145532.XA CN103215209B (en) 2013-04-24 2013-04-24 Bacillus amyloliquefaciens and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310145532.XA CN103215209B (en) 2013-04-24 2013-04-24 Bacillus amyloliquefaciens and application thereof

Publications (2)

Publication Number Publication Date
CN103215209A true CN103215209A (en) 2013-07-24
CN103215209B CN103215209B (en) 2014-12-31

Family

ID=48813373

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310145532.XA Expired - Fee Related CN103215209B (en) 2013-04-24 2013-04-24 Bacillus amyloliquefaciens and application thereof

Country Status (1)

Country Link
CN (1) CN103215209B (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104012530A (en) * 2014-06-16 2014-09-03 中国烟草总公司陕西省公司 Microbial dispersible tablet for preventing soil-borne diseases of tobacco field and preparation method of microbial dispersible tablet
CN104593284A (en) * 2014-09-06 2015-05-06 山西农业大学 Walnut endophyte and use thereof
CN105176889A (en) * 2015-10-26 2015-12-23 刘洪亮 Bacillus amyloliquefaciens and application thereof
CN105191988A (en) * 2015-10-26 2015-12-30 刘洪亮 Bacillus amyloliquefaciens preparation and application thereof
CN105368739A (en) * 2015-11-06 2016-03-02 山东省烟台市农业科学研究院 Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof
CN105585379A (en) * 2015-12-21 2016-05-18 云南省微生物发酵工程研究中心有限公司 Biological compound fertilizer and application thereof
CN106701634A (en) * 2017-01-22 2017-05-24 扬州大学 Bacillus amyloliquefaciens BN-1 and application
CN107245461A (en) * 2017-05-31 2017-10-13 北方民族大学 A kind of bacillus amyloliquefaciens B6 and its application
CN107345215A (en) * 2017-07-27 2017-11-14 上海万力华生物科技有限公司 One plant of Paenibacillus polymyxa and its application in powdery mildew of cucumber and downy mildew preventing and treating
CN108641982A (en) * 2018-05-09 2018-10-12 青海省农林科学院 A kind of Te Shi salt bacillus S61 and its application
CN110192486A (en) * 2019-04-24 2019-09-03 新疆林科院经济林研究所 A method of for preventing and treating jujube tree virosis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101423812A (en) * 2008-12-17 2009-05-06 河南省农业科学院 Bacillus amyloliquefaciens and microbiological preparation and preparation method thereof
CN102604864A (en) * 2012-03-09 2012-07-25 陕西加伦多作物科学有限公司 Microbial bactericide and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101423812A (en) * 2008-12-17 2009-05-06 河南省农业科学院 Bacillus amyloliquefaciens and microbiological preparation and preparation method thereof
CN102604864A (en) * 2012-03-09 2012-07-25 陕西加伦多作物科学有限公司 Microbial bactericide and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PENGCHAO ZHAO ET AL: "Bacillus amyloliquefaciens Q-426 as a potential biocontrol agent against Fusarium oxysporum f. sp. spinaciae", 《J. BASIC MICROBIOL.》 *
王军华: "芽孢杆菌Q-12的鉴定及其抗菌活性物质的研究", 《中国优秀硕士学位论文全文数据库》 *
葛平华 等: "海洋解淀粉芽孢杆菌GM-1菌株发酵液抗菌谱及稳定性测定", 《农药》 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104012530A (en) * 2014-06-16 2014-09-03 中国烟草总公司陕西省公司 Microbial dispersible tablet for preventing soil-borne diseases of tobacco field and preparation method of microbial dispersible tablet
CN104593284A (en) * 2014-09-06 2015-05-06 山西农业大学 Walnut endophyte and use thereof
CN104593284B (en) * 2014-09-06 2017-10-10 山西农业大学 One plant of Endophyte of walnut and its application
CN105191988A (en) * 2015-10-26 2015-12-30 刘洪亮 Bacillus amyloliquefaciens preparation and application thereof
CN105176889A (en) * 2015-10-26 2015-12-23 刘洪亮 Bacillus amyloliquefaciens and application thereof
CN105191988B (en) * 2015-10-26 2017-10-27 刘洪亮 A kind of bacillus amyloliquefaciens preparation and its application
CN105368739B (en) * 2015-11-06 2018-04-10 山东省烟台市农业科学研究院 The Bacillus amyloliquefaciens strain of wide spectrum resistance fruit disease fungus and its application
CN105368739A (en) * 2015-11-06 2016-03-02 山东省烟台市农业科学研究院 Bacillus amyloliquefaciens strain capable of resisting fruit tree pathogenic fungi in broad spectrum and application thereof
CN105585379A (en) * 2015-12-21 2016-05-18 云南省微生物发酵工程研究中心有限公司 Biological compound fertilizer and application thereof
CN106701634A (en) * 2017-01-22 2017-05-24 扬州大学 Bacillus amyloliquefaciens BN-1 and application
CN106701634B (en) * 2017-01-22 2019-12-03 扬州大学 Bacillus amyloliquefaciens BN-1 and application
CN107245461A (en) * 2017-05-31 2017-10-13 北方民族大学 A kind of bacillus amyloliquefaciens B6 and its application
CN107245461B (en) * 2017-05-31 2020-09-29 北方民族大学 Bacillus amyloliquefaciens B6 and application thereof
CN107345215A (en) * 2017-07-27 2017-11-14 上海万力华生物科技有限公司 One plant of Paenibacillus polymyxa and its application in powdery mildew of cucumber and downy mildew preventing and treating
CN107345215B (en) * 2017-07-27 2020-07-03 上海万力华生物科技有限公司 Paenibacillus polymyxa and application thereof in prevention and treatment of powdery mildew and downy mildew of cucumber
CN108641982A (en) * 2018-05-09 2018-10-12 青海省农林科学院 A kind of Te Shi salt bacillus S61 and its application
CN110192486A (en) * 2019-04-24 2019-09-03 新疆林科院经济林研究所 A method of for preventing and treating jujube tree virosis

Also Published As

Publication number Publication date
CN103215209B (en) 2014-12-31

Similar Documents

Publication Publication Date Title
CN103865843B (en) A kind of microbial composite bacteria and the application in control vegetable fungi disease thereof
CN101423812B (en) Bacillus amyloliquefaciens and microbiological preparation and preparation method thereof
US8518428B2 (en) Antagonistic bacteria for controlling the Fusarium wilt of continuous cropping banana and their microbial organic fertilizer
CN104762223B (en) A kind of bacillus amyloliquefaciens BA-KA3 and its application
CN101659932B (en) Antagonistic bacteria preventing and removing continuous cropping tobacco bacterial wilt and microbial organic fertilizer thereof
CN105132336B (en) One bacillus amyloliquefaciens and its microbial inoculum and application
CN102776130B (en) Metarhizium anisopliae and application thereof
CN100334201C (en) Bacillus subtilis and its uses
CN103695356B (en) A kind of bacillus amyloliquefaciens and microbiobacterial agent and application thereof
CN101697736B (en) Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof
CN105018366B (en) Methylotrophic bacillus and its application
CN105586298B (en) A kind of application of bacillus subtilis, microbial inoculum, the preparation method of microbial inoculum and microbial inoculum
CN101759494B (en) Diseases-prevention organic fertilizer prepared by edible fungi residue
CN102433282B (en) Bacillus subtilis NB12, as well as culture method and application thereof
CN102952768B (en) Bacillus, bacterial agent, preparation method and applications thereof
CN101575574B (en) Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection
KR20130038057A (en) A method for cultivating crop comprising bioactive material using a fermented broth of herbal medicine by combined microorganisms, and a crop comprising bioactive material prepared by the method
CN104164394B (en) The bacterial strain of one strain antagonistic phytopathogen and application thereof
CN101864378B (en) Streptomyces microflavus
CN101781569B (en) Soil improvement and anti continuous cropping multifunctional biological agent
CN105483054B (en) A kind of bacillus amyloliquefaciens WS-8 and its application
CN102910988A (en) Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer
CN101961014B (en) Method for preparing microbial pesticide for preventing and controlling fungal diseases of vegetables
CN102296036B (en) Bacillus pumilus and application in disease prevention and growth promotion thereof
CN100445370C (en) Bacillus subtilis, bacterium agent and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model
TR01 Transfer of patent right

Effective date of registration: 20150714

Address after: No. 1888, Weifang Economic Development Zone, Shandong Friendship Road

Patentee after: Weifang Wansheng Biological Pesticide Co., Ltd.

Address before: 250014 Ji'nan Province, Lixia District, Shandong Province Cultural Road, No. 42

Patentee before: Niu Shanguang

Patentee before: Li Peng

Effective date of registration: 20150714

Address after: No. 1888, Weifang Economic Development Zone, Shandong Friendship Road

Patentee after: Weifang Wansheng Biological Pesticide Co., Ltd.

Address before: 250014 Ji'nan Province, Lixia District, Shandong Province Cultural Road, No. 42

Patentee before: Niu Shanguang

Patentee before: Li Peng

ASS Succession or assignment of patent right

Free format text: FORMER OWNER: LI PENG

Effective date: 20150714

Owner name: WEIFANG WANSHENG BIOLOGICAL PESTICIDE CO., LTD.

Free format text: FORMER OWNER: NIU SHANGUANG

Effective date: 20150714

C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20170330

Address after: Shandong Province, Qingdao City, Laixi City Zhen Ma Tai Cun he tou.

Patentee after: Qingdao Taiwan Baer Biological Engineering Co. Ltd.

Address before: No. 1888, Weifang Economic Development Zone, Shandong Friendship Road

Patentee before: Weifang Wansheng Biological Pesticide Co., Ltd.

Effective date of registration: 20170330

Address after: Shandong Province, Qingdao City, Laixi City Zhen Ma Tai Cun he tou.

Patentee after: Qingdao Taiwan Baer Biological Engineering Co. Ltd.

Address before: No. 1888, Weifang Economic Development Zone, Shandong Friendship Road

Patentee before: Weifang Wansheng Biological Pesticide Co., Ltd.

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20141231

Termination date: 20190424