CN106591196A - Photosynthetic bacterium strain and its use in prevention and treatment on clubroot of cruciferous crops - Google Patents
Photosynthetic bacterium strain and its use in prevention and treatment on clubroot of cruciferous crops Download PDFInfo
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- CN106591196A CN106591196A CN201611252810.1A CN201611252810A CN106591196A CN 106591196 A CN106591196 A CN 106591196A CN 201611252810 A CN201611252810 A CN 201611252810A CN 106591196 A CN106591196 A CN 106591196A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/205—Bacterial isolates
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention relates to a photosynthetic bacterium strain for preventing and treating clubroot of cruciferous crops and its application and belongs to the technical field of plant protection. The photosynthetic bacterium belongs Lampropedia sp, is named as photosynthetic bacterium GH-1, is preserved in the China center for type culture collection on November 25, 2016 and has a preservation number of CCTCC NO: M 2016680. The strain has high ability to effectively prevent and treat clubroot of cruciferous crops, can be used for preventing and treating clubroot of cruciferous crops, reduces clubroot morbidity to about 10%, observably reduces a plant disease index, effectively improves a crop yield, has a yield increasing rate of 150% or more, is environmentally friendly and safe, does not produce residues and broadens the application field of photosynthetic bacteria.
Description
Technical field
The present invention relates to one plant of photosynthetic bacteria and its application in preventing and treating crop in cruciferae clubroot, belong to microorganism
And plant biological Prevention Technique field.
Background technology
Crop in cruciferae includes Brassica campestris L and bulk vegetable, such as Chinese cabbage, Caulis et Folium Brassicae capitatae, Brassica oleracea L. var. botrytis L., Corm Brassicae caulorapae, Caulis et Folium Brassicae junceae, Radix Raphani and
Special crop Radix Isatidis and Wasabia japonic (Euterma Wasabi), be China's plantation most extensively, eat most common vegetable species, the Zhejiang of China, Guangdong,
Guangxi, Sichuan, Anhui, Hunan, Jiangxi, Fujian, Jiangsu, Yunnan, Xinjiang, Tibet, Liaoning, Shandong, Shanghai, Beijing and Chongqing
It is distributed Deng area.But crop in cruciferae easily infects clubroot, there is every year the area of clubroot up to 20,000,000 in China
It is more than mu, and extremely difficult preventing and treating, badly influence the productive life and economic well-being of workers and staff of peasant.
The main prophylactico-therapeutic measuress that Cruciferae clubroot is taken are had at present：Agricultural cultivation measure, disease-resistant variety, chemistry are anti-
Measure is controlled, but its prevention effect is undesirable.
Existing document report carries out preventing and treating clubroot using biological control method, and the such as B of patent CN 101416641 is reported
A kind of biological preparation of preventing and treating Cruciferae clubroot, the production bacterial strain of the biological preparation is bacillus amyloliquefaciens
(Bacillus subtilis) XF-1, the bacterial strain is obtained by liquid and solid fermentation culture, and the biological preparation is applied to into ten
The preventing and treating of Zi Hua sections clubroot, can be good at controlling the generation of clubroot, and substantially, prevention effect is reachable for production-increasing function
84.8%.The B of patent CN 102899266 provides a kind of Rhizoma amorphophalli endogenetic bacteria pantoea agglomerans bacterial strain 1-7 and its is preparing cross
Application in flower section vegetable clubroot preparation, the features such as with efficient, nontoxic and good growth-promoting effect.Patent CN 105296392
A reports one plant and has the few biocontrol bacterial strain of efficient prophylactic-therapeutic effect and application dosage to rapeseed clubroot, and the bacterial strain is solution starch
Bacillus cereuss Bam22, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and preserving number is CGMCC
No.11329。
At present for photosynthetic bacteria is successfully applied to the preventing and treating of crop in cruciferae clubroot there is not yet pertinent literature report.
The content of the invention
For deficiency of the prior art, an object of the present invention is just to provide one plant and can be applied to prevent and treat cruciate flower
The photosynthetic bacteria of section's clubroot, can efficiently prevent and treat Cruciferae clubroot, reduce crop sickness rate, and can preferably promote crop
Volume increase.
The present inventor is through substantial amounts of experimentation and gropes, and obtains one plant of photosynthetic bacteria, the photosynthetic bacteria
For Lampropedia (Lampropedia sp.), it is named as photosynthetic bacteria GH-1 (Lampropedia hyaline GH-1),
China typical culture collection center is preserved on November 25th, 2016, collection address is：China. Wuhan. Wuhan is big
Learn, postcode：430072, collection deposit number is CCTCC NO：M 2016680.
The second object of the present invention is to provide a kind of application of photosynthetic bacteria in preventing and treating crop in cruciferae clubroot.
The third object of the present invention is to provide a kind of photosynthetic bacteria in the biology for preparing preventing and treating crop in cruciferae clubroot
Application in terms of preparation.
Photosynthetic bacteria in the present invention includes following step when carrying out preventing and treating crop in cruciferae clubroot as biological preparation
Suddenly：
(1) biological preparation containing the photosynthetic bacteria described in claim 1 is prepared；
(2) step (1) gained biological preparation is administered using one of following methods：1. in admixing base fertilizer or topdressing
Apply；2. mix and applied in seed；3. add water to pour and apply in crop root.
The concentration of photosynthetic bacteria contained photosynthetic bacteria when biological preparation is prepared of the present invention is 6～9 × 108cfu/mL。
Above-mentioned photosynthetic bacteria is applied to prevent and treat crop in cruciferae clubroot, with efficient prophylactic-therapeutic effect, can be notable
The incidence rate of crop in cruciferae clubroot is reduced, and crop yield can be promoted, and it is environmentally friendly.As shown in the Examples, will
The photosynthetic bacteria of the present invention makes the clubroot aspect for being applied to prevent and treat crop in cruciferae Chinese cabbage after preparation, the sickness rate of crop
10% or so is reduced to, average disease index is reduced to 9.4,1900 kilograms per mu of crop yield.
The beneficial effects of the present invention is：Obtained strains of the present invention have efficient preventing and treating crop in cruciferae clubroot
Ability, is applied in the preventing and treating of Cruciferae clubroot, and the sickness rate of clubroot can be reduced to 10% or so, and significantly reduce plant
The disease index of strain, while crop yield can be effectively improved, rate of growth is up to more than 150%；Photosynthetic bacteria in the present invention is to ring
Border is friendly, using safety, noresidue；The present invention has also widened the application of photosynthetic bacteria.
Description of the drawings
Fig. 1 is the growth promoter of seedling after 300 times of post processing Chinese cabbage seeds of photosynthetic bacteria GH-1 preparation diluents in embodiment 1
Compares figure；
Wherein, A sides are processed to employ photosynthetic bacteria GH-1 preparations, and B sides are processed to be provided without photosynthetic bacteria GH-1 preparations.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to it is pointed out here that be that following examples are simply used
In being further detailed to the present invention, it is impossible to be interpreted as limiting the scope of the invention, the field is skilled in technique
Personnel still fall within protection scope of the present invention according to some nonessential modifications and adaptations that foregoing invention content is made.
Embodiment 1
1st, experimental raw and method
1.1 bacterium source
Slight disease plant rhizosphere in the serious field of Guanghan clubroot morbidity.
1.2 culture medium
Culture medium prescription is：Peptone 10g, yeast extract 5g, Sodium Chloride 10g, water 1000mL, pH is adjusted to 5.0.
The preservation of 1.3 bacterial strains
By in the glycerol of inoculation 25%, -70 DEG C or Liquid nitrogen storage, monthly subcultivation is once.
The identification of 1.4 bacterial strains
The DNA of antibacterial is extracted, 16S rDNA sequence analysis are carried out by PCR methods, gene order is shown in sequence table.By gained sequence
It is listed in NCBI websites to compare, and is named as photosynthetic bacteria GH-1.
Application of 1.5 bacterial strains in preventing and treating crop in cruciferae clubroot
Obtained strains are prepared into after test preparation, using root method is poured, direct root irrigation or dilute are carried out at seeding time
The method for releasing rear pouring root, the concentration of bacterial strain is 6～9 × 10 in undiluted bacterium solution preparation8Cfu/mL, is poured every time with per plant
100mL bacterium solutions carry out root irrigation, at least process 2 times, and to Chinese cabbage and Brassica campestris L field efficiency test and simulation experiments are carried out respectively.
The severity Scaling standard and investigation method of Chinese cabbage and rapeseed clubroot is carried out in the following manner：
Chinese cabbage severity Scaling standard is：
0 grade：Root is without tumor；
1 grade：Main root enlargement, its diameter is less than two times of basal part of stem；
3 grades：Main root enlargement, 2～3 times of its a diameter of basal part of stem；
5 grades：Main root enlargement, 3～4 times of its a diameter of basal part of stem；
7 grades：Main root enlargement, there is blackening in more than 4 times of its a diameter of basal part of stem or the root of enlargement.
Brassica campestris L severity Scaling standard is：
0 grade：Root is without tumor, and root system development is normal；
1 grade：The root of 1-30% a little tumor of life, there is little nodositas lump, is mutually not connected to；
2 grades：The root of 31-60% raw tumor, there is big nodositas lump, is connected with each other；
3 grades：The root of 61-100% raw tumor, expands formation spindle tumor, and big tumor mass extends to hypocotyls,
Plant is short and small, stops growing.
Disease index, sickness rate and prevention effect are calculated as follows：
Sickness rate (%)=(morbidity strain number/investigation total strain number) × 100；
(1) it is as follows to the result of the test of Chinese cabbage：
Photosynthetic bacteria is used for into the controlling experiment of cabbage clubroot as biological preparation, as a result as shown in table 1：
Table 1
Photosynthetic bacteria is used to as biological preparation to prevent and treat the simulation experiments of cabbage clubroot, as a result as shown in table 2：
Table 2
| Process | Yield (kg/ mus) | Volume increase (kg/ mus) | Rate of growth (%) |
| CK | 900 | — | — |
| 100 times of dilution | 2800 | 1900 | 211.1 |
| 300 times of dilution | 2400 | 1500 | 166.7 |
| 500 times of dilution | 1950 | 1050 | 116.7 |
| 70% 800 times of Bravo | 1370 | 470 | 52.2 |
As can be seen from Table 1 and Table 2, the present invention is used to process Chinese cabbage using the biological preparation containing photosynthetic bacteria, in preventing and treating
Cabbage clubroot and Chinese cabbage volume increase aspect serve good effect, dilute 100 times of preventive effect up to 87%, and rate of growth is
211.1%.
Root irrigation is carried out after photosynthetic bacteria preparation is diluted into 300 times, the growth promoter situation such as Fig. 1 institutes to Chinese cabbage seedling
Show, wherein A sides are that, using photosynthetic bacteria preparation root irrigation, B sides are control, it is seen that the Chinese cabbage growth of seedling of A sides is vigorous.
(2) it is as follows to the result of the test of Brassica campestris L：
Photosynthetic bacteria is used for into the controlling experiment of rapeseed clubroot as biological preparation, as a result as shown in table 3：
Table 3
Photosynthetic bacteria is used to as biological preparation to prevent and treat the simulation experiments of rapeseed clubroot, as a result as shown in table 4：
Table 4
From table 3 and table 4 as can be seen that the preparation containing photosynthetic bacteria of the present invention is preventing and treating rapeseed clubroot and rape yield increasing
Aspect serves good effect, and the preventive effect for diluting 300 times is 80.4%, and rate of growth is 130.9%.
SEQUENCE LISTING
<110>Inst of Plant Protection, Sichuan Academy of Agricultural Sciences
<120>One plant of photosynthetic bacteria and its application in preventing and treating Cruciferae clubroot
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1224
<212> DNA
<213>Photosynthetic bacteria
<400> 1
gactgcaact gagtgtatcg ctccttgcgg tccgaacttc aggcaccccc ggcttccatg 60
gcttgacggg cggtgtgtac aaggcccggg aacgtattca ccgcgccatg gctgatgcgc 120
gattactagc gaatccaact tcacggagtc gagttgcaga ctccgatccg aactgagata 180
ggtttttgag atttgcgcac catcgctggt taccggccct ctgtacctac cattgtaaca 240
cgtgtgtcgc cccggacgta agggccgtgc tgatttgacg tcctcccccc cttcctcaca 300
ccttacggtg gcagtctcgt tagagttccc agcttgacct gatggcaact aacgacaagg 360
gttgcgctcg ttatggcact taagccgaca cctcacggca cgagctgacg acaaccatgc 420
agcacctcga tacacactat tgctagctat gatatctcta ccatattcct gcaccgttcg 480
agcccgggta aggttcctcg cgtatcatcg aattaaacca catgttcctc cgcttgtgcg 540
ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt actccccagg tggataactt 600
atcgctttcg cttggtcact gactgtgtat cgccaacaac tagttatcat cgtttacagt 660
gtggactacc agggtatcta atcctgtttg atccccacac tttcgtgcct cagcgtcaat 720
aatggctttg tgagctgcct tcgcaatcga tattctgtgt gatatctaag catttcaccg 780
ctacaccaca cattccactc acagcaacca tattctagcc tgacagtatc aacggcattt 840
ttccagttaa gctgaaaact ttcaccactg acttatcaga ccgcctacgc accctttaaa 900
cccaataaat ccggataacg ctcgcatcct ccgtattacc gcggctgctg gcacggagtt 960
agccgatgct tattcatatg gtactctcat cagtcttcgc aaagacctta attgctccca 1020
tataaagcag tttacaatcc cataggaccg tcttcctgct acgcagcatg ctggatcaga 1080
ttcccatcca ttgtccgata tcctcactgc tgacctcctc gtagagtctg ggaccgtgtc 1140
tccagtccag tgtgggcgat cttcctctcg aatcctagac atctagtcat gtaggtcggt 1200
tacctacatc taacttaagt gtcc 1224
Claims (5)
1. one plant of photosynthetic bacteria, it is characterised in that the photosynthetic bacteria is Lampropedia Lampropedia sp., is named as
Photosynthetic bacteria GH-1, depositary institution is：China typical culture collection center, collection is numbered and is：CCTCC NO：M
2016680, the preservation time is：On November 25th, 2016.
2. application of the photosynthetic bacteria described in claim 1 in preventing and treating crop in cruciferae clubroot.
3. the photosynthetic bacteria described in claim 1 is in answering that preparation is used in the biological preparation for preventing and treating crop in cruciferae clubroot
With.
4. application according to claim 2, it is characterised in that the application comprises the steps：
(1) biological preparation containing the photosynthetic bacteria described in claim 1 is prepared；
(2) step (1) gained biological preparation is administered using one of following methods：1. base fertilizer is admixed or middle administration of topdressing；
2. mix and applied in seed；3. add water to pour and apply in crop root.
5. application according to claim 4, it is characterised in that in the biological preparation photosynthetic bacteria concentration be 6～9 ×
108cfu/mL。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611252810.1A CN106591196B (en) | 2016-12-30 | 2016-12-30 | One plant of photosynthetic bacteria and its application in prevention and treatment Cruciferae clubroot |
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| CN201611252810.1A CN106591196B (en) | 2016-12-30 | 2016-12-30 | One plant of photosynthetic bacteria and its application in prevention and treatment Cruciferae clubroot |
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| CN106591196A true CN106591196A (en) | 2017-04-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113249274A (en) * | 2021-05-24 | 2021-08-13 | 齐鲁工业大学 | Junpian bacteria and junpian bacteria cell suspension and application thereof in preventing and treating pepper wilt |
| CN113249248A (en) * | 2021-04-25 | 2021-08-13 | 西华大学 | Gordonia melanodendri, application and pigment production method |
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| CN103667134A (en) * | 2013-12-10 | 2014-03-26 | 江苏省农业科学院 | Paenibacillus polymyxa N3-4 used for preventing and treating clubroot of cruciferous crops and application thereof |
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| CN105875649A (en) * | 2016-05-10 | 2016-08-24 | 长江师范学院 | Application of photosynthetic bacteria in tuber mustard clubroot prevention and control and tuber mustard clubroot prevention and control agent |
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2016
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| Title |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113249248A (en) * | 2021-04-25 | 2021-08-13 | 西华大学 | Gordonia melanodendri, application and pigment production method |
| CN113249274A (en) * | 2021-05-24 | 2021-08-13 | 齐鲁工业大学 | Junpian bacteria and junpian bacteria cell suspension and application thereof in preventing and treating pepper wilt |
| CN113249274B (en) * | 2021-05-24 | 2022-03-11 | 齐鲁工业大学 | Junpian bacteria and junpian bacteria cell suspension and application thereof in preventing and treating pepper wilt |
| WO2022246954A1 (en) * | 2021-05-24 | 2022-12-01 | 山东省科学院生态研究所 （山东省科学院中日友好生物技术研究中心） | Lampropedia strain and application thereof in prevention and control of capsicum fusarium wilt |
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| CN106591196B (en) | 2019-08-13 |
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