CN1319459C - Coptis pesticide synergist - Google Patents
Coptis pesticide synergist Download PDFInfo
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- CN1319459C CN1319459C CNB2005100206005A CN200510020600A CN1319459C CN 1319459 C CN1319459 C CN 1319459C CN B2005100206005 A CNB2005100206005 A CN B2005100206005A CN 200510020600 A CN200510020600 A CN 200510020600A CN 1319459 C CN1319459 C CN 1319459C
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Abstract
The present invention relates to a coptis pesticide synergistic agent which comprises 1 portion of leaves or stems of coptis, a hydrochloric acid or sulfuric acid solution which accounts for 2 to 10 times of the volume and additives as the rest, wherein the concentration of the hydrochloric acid or sulfuric acid solution is from 0.01% to 5%. A preparation method comprises the steps: after pulverized, the leaves and/or the stems of the coptis are soaked and extracted for 3 to 20 hours by the acid solution which accounts for 2 to 10 times of the volume at 20 to 100 DEG C, and then the solution is separated. After the solution is properly concentrated, the additives having stabilizing and dispersing functions are added, and the pesticide synergistic agent which can resist bacteria, viruses and pests is prepared. The product fully uses the functions for suppressing bacteria and resisting viruses of alkaloid of the by-products of the coptis, and particularly the synergic synergistic effect of the alkaloid of the coptis and pesticide which can resist bacteria and viruses is used. After the coptis pesticide synergistic agent is compounded with other pesticide which can resist bacteria and viruses, the bacterium suppressing effect of the pesticide can be obviously improved. The present invention has the advantages of low cost, simple processing processes and obvious synergistic effect of the obtained products.
Description
Technical field
The invention belongs to the pesticide synergistic agent technology, be specifically related to a kind of coptis pesticide synergist.
Background technology
Coptis bitter, cold in nature.The function that purging intense heat, detoxifcation, heat-clearing, eliminating dampness are arranged.Be used for that dysphoria with smothery sensation coma, vexed insomnia, damp and hot ruffian are full, vomiting, bellyache and diarrhea dysentery, hot eyes pyogenic infections, aphthae, eczema, scald, haematemesis, bleeding from five sense organs or subcutaneous tissue etc.The coptis beginning is stated from Shennong's Herbal, classifies as top grade.The various coptiss mainly all contain jamaicin (Berberine), and fork claims berberine, content 5.20～7.69%.Other still contains coptisine (Coptise), worenine (Worenine), palmatine (Palmatine), jateorrhizine (Jatrorrhizine), and all each because they have analog structure, is referred to as the coptis root alkaloid below 0.5% to the content of these alkali.The alkaloid of the coptis is coughed bacillus, typhoid bacillus, tubercle bacillus, staphylococcus aureus, hemolytic streptococcus, Diplococcus pneumopniae and some fungies (Candida albicans) to shigella dysenteriae, cholera bacteria, daytime all obvious suppression effect (Xiao Ping, " pharmacological action of the coptis and clinical practice ", Chinese Medical Journal (Beijing), 2004,6 (6): 142～143).The intensity of external anti-staphylococcus aureus 209P and sinomin (CM
2) similar.The coptis or jamaicin and streptomycin, yellow amine have synergy to staphylococcic inhibition.
The coptis is as rare Chinese medicine, and market conditions are well sold and in short supply.But the accessory substance of the coptis (coptis leaf and stem stalk etc.) is wasted entirely, does not utilize.1., coptis leaf contains total alkaloids 6% our result of network analysis shows:; Wherein, jamaicin about 1.4%, jateorrhizine about 0.2%, palmatine about 0.8%; In addition, contain 12% nutrient components such as amino acid in addition.2., coptis stem stalk contains total biology broken 3%; Wherein, jamaicin about 0.8%, palmatine about 0.6%; In addition, contain 8% nutrient components such as amino acid (seeing Table 1) in addition.Has higher value.
The active ingredient of table 1, coptis plant and nutrient component
| Jamaicin (Berberine) | Palmatine (Palmatine) | Jateorrhizine (Jatrorrhizine) | Total alkaloids | Amino acid | |
| Coptis leaf | 1.47 | 0.21 | 0.78 | 5.71 | 12.18 |
| Coptis stalk | 0.79 | 0.1 | 0.61 | 3.18 | 7.93 |
(inhibited to active component (comprising various alkaloids), medicine composition, the pharmacological action of the coptis at present to those microorganisms and virus, those diseases are had the size of therapeutic action and result of treatment etc.), the cultivation portions such as (cultivation management measures) sufficient research is arranged, abundant research document and data are arranged.The active component of coptis leaf also was studied, and relevant bibliographical information is also arranged.With the coptis is the various products of development of raw materials, and kind is very many, mainly prepares clearing heat and detoxicating Chinese patent drug and Chinese medicine and makes up a prescription etc.But, be the product of development of raw materials with coptis leaf, do not appear in the newspapers as yet (because conventional utilization is the comparison difficulty); More do not see any report that is developed as the coptis pesticide synergist aspect.
Summary of the invention
The objective of the invention is to defective at the prior art existence, a kind of coptis pesticide synergist is provided, accessory substance (coptis leaf and coptis stalk) with the coptis is a primary raw material, by extracting processing, be developed as the synergist that sterilization (antibiotic) and antiviral agricultural chemicals is played the coptis pesticide of remarkable synergistic effect.
The composition of this coptis pesticide synergist has: 1 part of coptis leaf and/or cane, 2～10 times of volumes of 0.01～5% hydrochloric acid or sulfuric acid solution liquid, auxiliary agent surplus.Method for making is: after coptis leaf and/or cane pulverizing, acid solution with 2～10 times of volumes soaks extraction 3～20 hours down at 20～100 ℃, isolates solution, after suitably concentrating, add auxiliary agent, be antibiotic, antiviral and pest-resistant pesticide synergistic agent with stable and peptizaiton.
Wherein auxiliary agent can adopt Tween series auxiliary agent, also can adopt other to have the agricultural chemicals usual auxiliaries of non-ionic surface active effect.
The present invention has utilized accessory substance one coptis leaf and the cane of the coptis, and its active ingredient is coptis alkaloid, has sterilization and suppresses viral effect, is the main active of coptis pesticide synergist.Utilize acid solution to extract relevant active ingredient in the preparation from coptis plant, add auxiliary agent again, the stability of raising coptis active component and water-soluble is convenient to the moulding and the development of preparation.
Below be the contrast experiment of the synergistic effect of relevant coptis synergist:
After 1 kilogram of coptis leaf and cane oven dry, pulverize.Add 5 liters of 0.5% sulfuric acid solution liquid, 50 ℃ of following stirring and leaching 5 hours.Filter, obtain extract, adjusting extract is 5 to pH.Extract is concentrated to about 1 liter, adds 10 gram Tween80.Be antibiotic pesticide synergistic agent.
In the agricultural streptomycin solution of 100 milliliters of 1ppm, add 0.1 milliliter of the concentrate of this extract, utilize the inhibition zone test method(s), detect bacteriostatic activity to microorganisms such as gold-coloured staphylococci, hay bacillus, Ralstonia solanacearums; With the coptis leaf of the streptomycin of 5ppm and 0.1% and the concentrate of cane extract is contrast.
The method that suppresses microbial activity is paper disk method (Ma Xurong etc.Medicine Experiment on Microbiology handbook [M].Beijing: People's Health Publisher .2001): microorganisms such as staphylococcus aureus, cultivate 24h prior to 37 ℃ (27 ℃ in yeast) on the slant medium, to get two then and encircle and in broth bouillon, cultivate 6～8h, microscopically is observed counting, adjusts to contain bacterium 10
5-10
6/ ml is standby.Aseptic agar medium is poured in the 9cm glass dish, and every 12ml is standby; After the sterilization of the diameter 5mm circle scraps of paper, be immersed in the soup of variable concentrations standby.50 μ l bacterium liquid are evenly coated the top layer of agar medium, then the pastille scraps of paper are pasted on the agar surface, put the diameter that 37 ℃ (27 ℃ in yeast) cultivates amount inhibition zone behind the 24h.Every drug concentration pastes three.Average.Antibacterial circle diameter is big more, and bacteriostasis is strong more.
Table 2, coptis leaf and cane extract bacteriostatic experiment result and synergism experiment result (antibacterial circle diameter millimeter)
| Sample | Gold-coloured staphylococci | Hay bacillus | Ralstonia solanacearum |
| The 5ppm streptomycin | 16 | 18 | 19 |
| 0.1% coptis synergist | 7 | 6 | 7 |
| 1ppm streptomycin+0.1% coptis synergist | 17 | 18 | 20 |
The present invention has the following advantages:
1., this product is present unemployed coptis plant, is twice laid, low cost product, process is simple, is easy to preparation.
2., the synergistic effect of this product is remarkable.The present invention has made full use of alkaloidal antibacterial and antiviral efficacy in the coptis plant, especially utilized the synergistic function of coptis alkaloid and antibiotic and antiviral agricultural chemicals, behind other antibiotic and antiviral agriculture chemical compoundings, can significantly improve the fungistatic effect of agricultural chemicals, comparative costs is cheap, make full use of the active ingredient of coptis plant, and given full play to its social economic value.
3., this product is biological pesticide synergistic agent series.This product is a plant extracts, and itself is little to the adverse effect of human body; Add in the agricultural chemicals, can reduce the consumption of agricultural chemicals, positive effect is arranged for reducing residue of pesticide.Help promoting the production of pollution-free food.
Embodiment
Embodiment 1:
1 kilogram of coptis leaf and or the cane oven dry after, pulverize.2 liters of the sulfuric acid solution liquid of adding 5%, stirring and leaching is 20 hours under the room temperature.Filter, obtain extract, adjusting extract is 4～7 to pH.After extract suitably concentrates, add Tween80 as auxiliary agent.Be antibiotic pesticide synergistic agent.This pesticide synergistic agent is added in right amount in the agricultural chemicals such as streptomycin, the bacteriostatic activity that can improve streptomycin is more than 5 times.
Embodiment 2
After 1 kilogram of coptis leaf and the cane oven dry, pulverize.Add 10 liters of 0.01% hydrochloric acid solutions, 90 ℃ of following stirring and leaching 3 hours.Filter, obtain extract, adjusting extract is 4～7 to pH.After extract suitably concentrates, add Tween60 as auxiliary agent.Be antibiotic pesticide synergistic agent.Add in the agricultural chemicals such as streptomycin for pesticide synergistic agent in right amount, the bacteriostatic activity that can improve streptomycin is more than 5 times.
Embodiment 3
After 1 kilogram of coptis leaf oven dry, pulverize.Add 5 liters of 0.5% sulfuric acid solution liquid, 60 ℃ of following stirring and leaching 8 hours.Filter, obtain extract, adjusting extract is 4～7 to pH.After extract suitably concentrates, add Tween20 as auxiliary agent.Be antibiotic pesticide synergistic agent.Add in the agricultural chemicals such as streptomycin for pesticide synergistic agent in right amount, the bacteriostatic activity that can improve streptomycin is more than 5 times.
Claims (2)
1, coptis pesticide synergist, it is or/and after the coptis cane raw material pulverizing with 1 part of Chinese goldthread leaf, hydrochloric acid or sulfuric acid solution liquid with 2-10 times of volume soak extraction, acid solutions 0.01～5%, concentrate after isolating solution, add auxiliary agent, promptly obtain antibiotic, antiviral pesticide synergistic agent, its main active is a coptis alkaloid.
2, coptis pesticide synergist according to claim 1 is characterized in that: auxiliary agent adopts Tween series auxiliary agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100206005A CN1319459C (en) | 2005-03-25 | 2005-03-25 | Coptis pesticide synergist |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100206005A CN1319459C (en) | 2005-03-25 | 2005-03-25 | Coptis pesticide synergist |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1709066A CN1709066A (en) | 2005-12-21 |
| CN1319459C true CN1319459C (en) | 2007-06-06 |
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ID=35705404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100206005A Expired - Fee Related CN1319459C (en) | 2005-03-25 | 2005-03-25 | Coptis pesticide synergist |
Country Status (1)
| Country | Link |
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| CN (1) | CN1319459C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994311B (en) * | 2016-06-22 | 2018-11-02 | 昆明百事德生物化学科技有限公司 | A kind of botanical pesticide and preparation method thereof of prevention tobacco bacterial wilt |
-
2005
- 2005-03-25 CN CNB2005100206005A patent/CN1319459C/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| "黄连生物碱对植物病原菌的抑菌作用及应用的初步研究" 朱振东，周繁茂，卫杨斗，华中农业大学学报，第10卷第4期 1991 * |
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| Publication number | Publication date |
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| CN1709066A (en) | 2005-12-21 |
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