A kind of anesthetic assistant
Technical field: the invention belongs to pesticide technology, be specifically related to a kind of anesthetic assistant.
Background technology: numb fruity hardship, cold in nature.The function that purging intense heat, detoxifcation, heat-clearing, eliminating dampness are arranged.Be used for that dysphoria with smothery sensation coma, vexed insomnia, damp and hot ruffian are full, vomiting, bellyache and diarrhea dysentery, hot eyes pyogenic infections, aphthae, eczema, scald, haematemesis, maggot blood etc.The fiber crops fruit beginning is stated from Shennong's Herbal, classifies as top grade.Various fiber crops fruits mainly all contain little ancient piece of jade, round, flat and with a hole in its centre alkali (Berberine), claim Hempfruit hormone again, content 5.20-7.69%.Other still contains fiber crops fruit alkali (Coptise), methyl fiber crops fruit alkali (worenine), palmatine (Pa lmatine), jateorrhizine (Jatrorrhizine), and the content of these alkali is all respectively below 0.5%, because they have analog structure, and general designation coptis root alkaloid.The alkaloid of fiber crops fruit is coughed bacillus, typhoid bacillus, tubercle bacillus, staphylococcus aureus, hemolytic streptococcus, Diplococcus pneumopniae and some fungies (Candida albicans) to shigella dysenteriae, cholera bacteria, daytime all obvious suppression effect (Xiao Ping, " pharmacological action and the clinical practice of fiber crops fruit ", Chinese Medical Journal (Beijing), 24,6 (6): 142-143).The intensity of external anti-staphylococcus aureus 209P and the different evil of sulfalene (CMZ) are similar.Fiber crops fruit or little poly-alkali and streptomycin, yellow amine have synergy to staphylococcic inhibition.The fiber crops fruit is as rare Chinese medicine, and market conditions are well sold and in short supply.But the accessory substance of fiber crops fruit (fiber crops fruit leaf and stem stalk etc.) is wasted entirely, does not utilize.1., fiber crops fruits leaf contains total alkaloids 6% our result of network analysis shows:; Wherein, little poly-alkali about 1.4%, jateorrhizine about 0.2%, palmatine about 0.8%; In addition, contain 12% nutrient components such as amino acid in addition.2., numb stem end stalk contains. total alkaloids 3%; Wherein, little case alkali about 0.8%, palmatine about 0.6%; In addition, contain 8% nutrient components such as amino acid (seeing Table 1) in addition.Has higher value.Table 1, the amino tingle fruit leaf 1.470.210.785.7112.18 fiber crops of the active ingredient of numb fruit accessory substance and the little poly-alkali of nutrient component (Berberine) palmatine (Pa lmatine) jateorrhizine (Jatrorrhizine) total alkaloids fruit stalk 0.790.10.613.187.93 is at present to fiber crops active component (comprising various alkaloids) really, medicine is formed, pharmacological action is (inhibited to those microorganisms and virus, those diseases had the size of therapeutic action and result of treatment etc.), cultivation (cultivation management measure) etc. all has sufficient research, and abundant research document and data are arranged.The active component of fiber crops fruit leaf also was studied, and relevant bibliographical information is also arranged.With fiber crops really is the various products of development of raw materials, and kind is very many, mainly prepares clearing heat and detoxicating Chinese patent drug and Chinese medicine and makes up a prescription etc.But, be the product of development of raw materials with fiber crops fruit leaf, do not appear in the newspapers as yet (because conventional utilization is the comparison difficulty); More do not see any report that is developed as the anesthetic assistant aspect.Summary of the invention the objective of the invention is to the defective at the prior art existence, a kind of anesthetic assistant is provided, accessory substance (fiber crops fruit leaf and fiber crops fruit stalk) with the fiber crops fruit is a primary raw material, by extracting processing, be developed as the synergist that sterilization (antibiotic) and antiviral agricultural chemicals is played numb orchard worker's medicine of remarkable synergistic effect.The composition of this anesthetic assistant has: 1 part of fiber crops fruit leaf and/or cane, 2-10 times of volume of the hydrochloric acid of 0.01-5% or sulfuric acid solution liquid, auxiliary agent surplus.Method for making is: after fiber crops fruit leaf and/or cane pulverizing, acid solution with 2-10 times of volume soaks extraction 3-20 hour down at 20-100 ℃, isolates solution, after suitably concentrating, add auxiliary agent, be antibiotic, antiviral and pest-resistant pesticide synergistic agent with stable and peptizaiton.Wherein auxiliary agent can adopt Tween series auxiliary agent, also can adopt other to have the agricultural chemicals usual auxiliaries of non-ionic surface active effect.The present invention has utilized accessory substance one fiber crops fruit leaf and the cane of numb fruit, and its active ingredient is fiber crops fruit alkaloid, has sterilization and the effect that suppresses virus, is the main active of anesthetic assistant.Utilize acid solution to extract relevant active ingredient in the preparation from fiber crops fruit accessory substance, add auxiliary agent again, the stability of raising fiber crops fruit active component and water-soluble is convenient to the moulding and the development of preparation.Below be contrast experiment: after 1 kilogram of fiber crops fruit leaf and cane oven dry, pulverize about the synergistic effect of fiber crops fruit synergist.Add 5 liters of 0.5% sulfuric acid solution liquid, 50 ℃ of following stirring and leaching 5 hours.Filter, obtain extract, adjusting extract is 5 to PH.Extract is concentrated to about 1 liter, adds 10 gram Tween80.Be antibiotic pesticide synergistic agent.In the agricultural streptomycin solution of 100 milliliters of lpPm, add 0.1 milliliter of the concentrate of this extract, utilize the inhibition zone test method(s), detect bacteriostatic activity to microorganisms such as gold-coloured staphylococci, hay bacillus, Ralstonia solanacearums; With the fiber crops fruit leaf of the streptomycin of spPm and 0.1% and the concentrate of cane extract is contrast.The method that suppresses microbial activity is paper disk method (Ma Xurong etc.Medicine Experiment on Microbiology handbook [M1.Beijing: People's Health Publisher .2001): microorganisms such as staphylococcus aureus, cultivate 24h prior to 37 ℃ (27 ℃ in yeast) on the slant medium, get two then and encircle cultivate 6-8h in broth bouillon, microscopically is observed counting, adjusts to that to contain bacterium 105-10 '/ml standby.Aseptic agar medium is poured in the gcm glass dish, and every 12ml is standby; After the sterilization of the diameter smm circle scraps of paper, be immersed in the soup of variable concentrations standby.50 river bacterium liquid are evenly coated the top layer of agar medium, then the pastille scraps of paper are pasted on the agar surface, put the diameter that 37 ℃ (27 ℃ in yeast) cultivates amount inhibition zone behind the 24h.Every drug concentration pastes three.Average.Antibacterial circle diameter is big more, and bacteriostasis is strong more.Table 2, fiber crops fruits leaf and cane extract bacteriostatic experiment result and synergism experiment result (antibacterial circle diameter millimeter) sample } gold-coloured staphylococci } 1., this product is present unemployed fiber crops fruit accessory substances { Ralstonia solanacearum sppm streptomycin 0.1% fiber crops fruit synergist lppm streptomycin+0.1% fiber crops fruit synergist the present invention's hay bacillus has the following advantages:, be twice laid, low cost product, process is simple, is easy to preparation.2., the synergistic effect of this product is remarkable.The present invention has made full use of alkaloidal antibacterial and antiviral efficacy in the numb fruit accessory substance, especially utilized the synergistic function of numb fruit alkaloid and antibiotic and antiviral agricultural chemicals, behind other antibiotic and antiviral agriculture chemical compoundings, can significantly improve the fungistatic effect of agricultural chemicals, comparative costs is cheap, make full use of the active ingredient of numb fruit accessory substance, and given full play to its social economic value.3., this product is biological pesticide synergistic agent series.This product is the forest extract, and itself is little to the adverse effect of human body; Add in the agricultural chemicals, can reduce the consumption of agricultural chemicals, positive effect is arranged for reducing residue of pesticide.Help promoting the production of pollution-free food.Embodiment embodiment 1:1 kilogram fiber crops fruit leaf and or the cane oven dry after, pulverize.2 liters of the sulfuric acid solution liquid of adding 5%, stirring and leaching is 20 hours under the room temperature.Filter, obtain extract, adjusting extract is 4-7 to PH.After extract suitably concentrates, add Tween80 as auxiliary agent.Be antibiotic pesticide synergistic agent.This pesticide synergistic agent is added in right amount in the agricultural chemicals such as streptomycin, the bacteriostatic activity that can improve streptomycin is more than 5 times.After 21 kilograms of fiber crops fruit leaves of embodiment and the cane oven dry, pulverize.Add 10 liters of 0.01% hydrochloric acid solutions, 90 ℃ of following stirring and leaching 3 hours.Filter, obtain extract, adjusting extract is 4-7 to PH.After extract suitably concentrates, add Tween60 as auxiliary agent.Be antibiotic pesticide synergistic agent.Add in the agricultural chemicals such as streptomycin for pesticide synergistic agent in right amount, the bacteriostatic activity that can improve streptomycin is more than 5 times.After the oven dry of 31 kilograms of fiber crops fruits of embodiment leaf, pulverize.Add 5 liters of 0.5% sulfuric acid solution liquid, 60 ℃ of following stirring and leaching 8 hours.Filter, obtain extract, adjusting extract is 4-7 to pH.After extract suitably concentrates, add Tween20 as auxiliary agent.Be antibiotic pesticide synergistic agent.Add in the agricultural chemicals such as streptomycin for pesticide synergistic agent in right amount, the bacteriostatic activity that can improve streptomycin is more than 5 times.