CN103783089A - Compound Chinese herbal medicine disinfectant for tableware and kitchenware disinfection - Google Patents
Compound Chinese herbal medicine disinfectant for tableware and kitchenware disinfection Download PDFInfo
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Abstract
The invention discloses a compound Chinese herbal medicine disinfectant for tableware and kitchenware disinfection, which consists of the following components in parts by weight: 0.025-0.100 part of common andrographis herb extract, 0.025-0.100 part of flos lonicerae extract, 0.025-0.100 part of radix isatidis extract and 0.032-2.000 parts of chitosan hydrochloride. The disinfectant disclosed by the invention has the advantages that the disinfectant has good killing effect and stability against the pathogenic bacteria of the intestinal tract; acute toxicity tests prove that the compound Chinese herbal medicine disinfectant belongs to practical non-toxic level substances; rat marrow osteocyte micronuclear tests prove that the compound Chinese herbal medicine disinfectant does not have genetic toxicity, has the characteristics of edibility, no toxin, no residue and the like, and can be used safely; the disinfectant realizes a remarkably high killing rate against natural bacteria on marine products, and is particularly suitable for the disinfection in the processing of cold fresh food of marine products.
Description
Technical field
The present invention relates to a kind of compound Chinese herb disinfectant, particularly a kind of compound Chinese herb disinfectant for tableware and kitchen tools sterilization
Background technology
Pathogenic microorganism and condition invasive organism can cause enteron aisle serious infectious diseases, the particularly eruption and prevalence of food poisoning through food, water etc., and You Yixia, spring, the season that fall temperature is higher are common.Pathogenic microorganisms can pollute tableware, causes food poisoning to occur through food cross pollution; Due to local flavor, process as main cold fresh food and can not adopt the method for thorough heating to carry out take aquatic products etc., there is a large amount of Case reports in the annual whole world such as food poisoning, pasracholera of thoroughly not causing due to heating disinfection.One of technical way of effectively controlling enteric infectious disease eruption and prevalence is the sterilization of food, tableware.
Traditional disinfectant refers to for killing pathogenic microorganism on communication media, make it reach the preparation of innoxious requirement, it is different from the antibiotics of clinical use, its Main Function in diseases prevention is that pathogenic microorganism is eliminated outside human body, cut off the route of transmission of infectious disease, reach the object of controlling infectious disease.People often claim that disinfectant is " chemosterilant ", mainly refer to that its effective composition is chemical substance, disinfectant take chemical substance as main active ingredient exists long, extended residual of environment degradable cycle to affect environment, broad-spectrum disinfectant sterilization environment and the micro-ecology of human body is exerted an influence etc., and then may affect organism metabolism and human health is produced to potential harmful effect.Owing to there is potential harm, traditional chemosterilant is unedible, and it is applied to table-ware disinfection, as punching does not wash clean easily, human body is worked the mischief.In recent years, the incidence of disease of crowd's tumour, metabolic disorder disease constantly rises.Therefore developmental research environmental protection, new disinfectant nontoxic, noresidue are the Important Problems of whole world preventive medicine circle research in recent years.It should be noted that, since 2004, the U.S. and European Union are successively studied with regard to Chinese Chinese herbal antibacterial material, explicitly pointed out medicinal herb components and have significant antibacterial bacteriostatic activity in correlative study report, and there is low toxicity, low-residual, the feature such as environmentally friendly, degradable.Over nearly 3 years, domesticly aspect novel edible disinfectant also carry out relevant research utilizing Chinese herbal medicine to prepare.
Document is recorded with clinical practice for many years and is shown, Radix Isatidis, honeysuckle, Herba Andrographitis three taste Chinese herbal medicine extracts all have effective antipathogenic composition, and the infusion of this three tastes Chinese herbal medicine has inhibitory action to staphylococcus aureus, hay bacillus, sarcine, typhoid bacillus, paratyphosum Bacterium, dysentery (will Hayes, Fu Shi) bacillus, bacillus enteritidis, hemolytic streptococcus etc.; In recent years research shows, the water soluble chitosan sterling that purity is 100% has very strong direct repression to the growth of many pathogens.
Summary of the invention
The object of this invention is to provide a kind of compound Chinese herb disinfectant for tableware and kitchen tools sterilization, this disinfectant has good killing effect and stability to pathogenic entero becteria, there is the features such as edible, nontoxic, noresidue, be particularly useful for the sterilization in the cold fresh food processing of marine product.
To achieve the above object of the invention, the present invention is by the following technical solutions:
This compound Chinese herb disinfectant for tableware and kitchen tools sterilization, it contains following component and weight consists of: 0.025～0.100 part of Andrographis Paniculata, 0.025～0.100 part of Honegsukle flower P.E, 0.025～0.100 part of isatis root extract, 0.032～2.000 part of chitosan hydrochloride.
Described contains in component, and its weight consists of: 0.100 part of Andrographis Paniculata, 0.100 part of Honegsukle flower P.E, 0.100 part of isatis root extract, 2.000 parts of chitosan hydrochlorides.
The formulation of disinfectant can be tablet.
Advantage of the present invention is: this disinfectant has good killing effect and stability to pathogenic entero becteria, acute toxicity experience proves, this compound Chinese herb disinfectant belongs to actual nontoxic level material, mouse marrow cell micro nuclear test shows, this compound Chinese herb disinfectant hereditary-less toxicity, has the features such as edible, nontoxic, noresidue, can relievedly use, disinfectant used is very high to the killing rate of natural bacteria on marine product, is particularly useful for the sterilization in the cold fresh food processing of marine product.
Embodiment
Embodiment
1. experiment material
1.1 disinfectant
Get respectively different amount Herba Andrographitis, honeysuckle, isatis root extract, chitosan hydrochloride, be mixed with disinfectant 1～No. 5 according to different formulations ratio, in table 1.Be dissolved in respectively in 100ml distilled water, after boiling sterilization, cooling for subsequent use.In each concentration disinfectant, active ingredient concentration is in table 2.
The compound Chinese herb disinfectant of table 1 variable concentrations proportioning
The each active ingredient concentration of compound Chinese herb disinfectant of table 2 variable concentrations
Said extracted thing and chitosan hydrochloride market are on sale, and extract can obtain by the extracting method of " Pharmacopoeia of People's Republic of China " 2010 editions.Chitosan hydrochloride adopts the acid-soluble rear removal free acid of common shitosan, dry after and get final product.
Andrographis Paniculata is purchased from Guanghan, Sichuan book on Chinese herbal medicine plant Co., Ltd, main active ingredient Determination of Andrographolide 5.4%; Honegsukle flower P.E is purchased from the Shandong biological Co., Ltd of gold space paulownia, main active ingredient chlorogenic acid (HPLC) content 5.06%; Isatis root extract is purchased from Nanjing Zelang Pharmaceutical Technology Inc., the main indigo content 2.3% of active ingredient.Chitosan hydrochloride, purchased from Qingdao Honghai Biotechnology Co., Ltd., is chitosan hydrochloride sterling, molecular weight 5-10 ten thousand.
1.2 experimental strain
Test with bacterium: staphylococcus aureus reference culture (ATCC 6538), Escherichia coli (ATCC 8099) are provided by Affiliated Hospital of Qingdao University microbiology teaching and research room's bacterial classification chamber, and vibrio parahemolyticus (ATCC 17802) is provided by Disease Control Center of Qingdao.
1.3 main chemical reagent and instruments (market is on sale)
2. experiment
2.1 infrastest liquid preparations
2.1.1 aseptic PBS buffer solution is got sodium hydrogen phosphate 2.89g, sodium dihydrogen phosphate 0.2g, sodium chloride 8.0g, potassium chloride 0.2g are dissolved in 1000ml distilled water, for subsequent use after high pressure steam sterilization.
2.1.2 nutrient agar is got 31g nutrient agar powder and is dissolved in 1000ml distilled water, and heating is boiled, before using through autoclaving.
2.1.3 bacterial suspension is inoculated in Escherichia coli, staphylococcus aureus on ordinary nutrient agar medium, vibrio parahemolyticus is inoculated in containing on the ordinary nutrient agar medium of 3.5% sodium chloride, putting 37 ℃ of incubators cultivates 24 hours, add the aseptic PBS buffer solution of 2-3ml wash lower lawn and dilute with aseptic PBS buffer solution, the experimental bacteria suspension of preparing variable concentrations is for subsequent use, and test organisms suspension bacteria containing amount is 1 × 10
7cfu/ml～5 × 10
7cfu/ml.
2.1.4 Ji's nurse Sa dye liquor is got Giemsa 3.8g, puts in agate mortar, adds a small amount of methyl alcohol and grinds.Add gradually methyl alcohol to 375ml, until completely dissolved, then add 125ml glycerine, put into 37 ℃ of incubators and be incubated 48h.Between soak, jolting for several times, makes abundant dissolving.Take out and filter, after 2 weeks, use, as Ji's nurse Sa dye liquor stoste.
When use, get 1 part of Ji's nurse Sa dye liquor stoste, mix with 9 parts of 1/15mol/L phosphate buffers (pH6.8), be made into its application liquid.
2.2 neutralizer choice experiments
Carry out the neutralizer choice experiment of novel compound Chinese herbal disinfectant.Experimental bacteria is Escherichia coli, and experiment is established parallel 6 groups, is undertaken by suspension quantitative bacteric idal experiment method.Experimental result, with the 6th group long bacterium, does not grow bacterium or bacterium number for the 1st group and is starkly lower than the 2nd group, and the 2nd group of clump count exceedes 100cfu/ml, and the 3rd, 4,5 groups of clump count errors are no more than 10%, show that selected neutralizer and concentration thereof are suitable.
2.3 suspension quantitative bacteric idal experiments
Get sterilizing test sterile test tube, first add 4.5ml disinfectant, then add 0.5ml test bacteria suspension, mix rapidly and clock immediately.Bacterium to be tested and disinfectant interacted to each scheduled time, drew respectively 0.5ml test organisms and disinfectant mixed liquor and added 4.5ml in the neutralizer of sterilizing, mixed.Each pipe test organisms and disinfectant mixed liquor, after adding neutralizer effect 10min, are drawn respectively 0.1ml sample liquid, cultivate method of counting mensuration deposit viable count by viable bacteria.When as more in the clump count of growing on flat board, can carry out after 10 times of dilutions of series, then carry out viable bacteria and cultivate counting.Replace thimerosal with PBS simultaneously, carry out parallel test, as positive controls.All test samples are all cultivated 48h and are observed final result in 37 ℃ of incubators.Test repeats 3 times, calculates the viable bacteria concentration (cfu/ml) of each group, and is scaled logarithm value (N), is then calculated as follows and kills logarithm value:
Kill logarithm value (No)-experimental group viable bacteria concentration logarithm value (Nx) of the average viable bacteria concentration of logarithm value (KL)=control group
When logarithm value is killed in calculating, get 2 significant digits value, can carry out Figure correction.Be less than or equal at 1 o'clock if sterilizing test group is disinfected rear average growth clump count, kill whole bacteriums, it kills logarithm value can be denoted as 5.00.
2.4 simple bactericidal assay
Totally 5 groups of compound Chinese herb disinfectant, Andrographis Paniculata, Honegsukle flower P.E, isatis root extract, chitosan hydrochlorides are set.Compound Chinese herb disinfectant is chosen No. 5 disinfectants that concentration is the highest, and simple concentration is pressed sanitizer formulations dosage No. 5, and each composition is dissolved in respectively in 100ml distilled water, the cooling rear use of boiling sterilization.Bacteria Indicators is chosen staphylococcus aureus, by suspension quantitative disinfecting test program, to staphylococcus aureus effect 5min.Each group test repeats 3 times, calculates and kills logarithm value.
2.5 organic substance influence bactericidal effect experiments
Organic substance influence experiment is in novel compound Chinese herbal disinfectant, to add variable concentrations calf serum, disinfectant mixes with volume ratio with calf serum for 1: 1 and 3: 1, carry out suspension quantitative bacteric idal experiment, with not containing organic matter control group relatively bactericidal effect change, to observe the impact of organic matter on bactericidal effect.After organic disinfectant and bacterial action certain hour, getting this mixed liquor 0.5ml adds in the test tube that fills 4.5ml neutralizer and mixes, neutralization 10min, getting this liquid 0.5ml is inoculated on ordinary nutrient agar medium, put 37 ℃ of incubators and cultivate 48h, counting bacterium colony also calculates it and kills logarithm value (KL).
2.6pH affects bactericidal effect experiment
PH group is set, pH-2 group, pH+2 group, totally 3 groups.Get the disinfectant solution preparing, first measure its pH value, in adjustment process, slowly drip hydrochloric acid (HCl) solution when meta-alkali, when meta-acid, slowly drip sodium hydroxide (NaOH) solution, measure at any time the pH of disinfectant solution.Regulate former disinfectant solution pH to each group of desired pH, stop adjusting, with minimal effective concentration disinfectant and the shortest action time, test by suspension quantitative disinfecting test program.If desired, after pH adjusts, can measure active constituent content and whether be subject to observe the impact that pH changes.Each group test repeats 3 times, calculates and kills logarithm value, detects the impact of pH on compound Chinese herb sanitizer disinfecting effect.
2.7 stability test
Compound Chinese herb disinfectant is the disinfectant take plant as main active ingredient, therefore using microbe method is carried out stability experiment.By 1～No. 5 disinfectant solution sealing lucifuge packing preparing, adopt accelerated test, put 54 ℃ of insulating boxs and deposit 14d, before depositing, test by suspension quantitative disinfecting test program after depositing, each group test repeats 3 times, calculates and kills logarithm value respectively.Except measuring Germicidal Efficacy, also answer observed and recorded disinfectant to have or not change color, have or not precipitation or suspension to produce.
2.8 marine product sterilizing tests
2.8.1 shellfish
New fresh scallops, oyster, purchased from marine product market, Qingdao, first wash down shellfish shell with flowing water, and in seam, incision slowly, pries open cap, then take out entire content with sterilizing tweezers, take in right amount in the mortar of sterilizing, are cut into small pieces and grind with scissors.Take 2g and be organized in sterilized empty bottle, add 18ml disinfectant (control group adds the physiological saline of equivalent) and be placed on oscillator it is mixed.Act on respectively to stipulated time (5min, 15min, 30min), get in the neutralizer solution that 0.5ml aforesaid liquid adds 4.5ml sterilizing and mix.After neutralization 10min, get this liquid of 0.1ml and be inoculated in ordinary nutrient agar medium, be placed in after 30 ℃ of incubators are cultivated 48h and make count plate, detect its sterilizing rate.Test repeats 3 times, averages.
2.8.2 fish
Fresh yellow croaker and flower tongue fish are purchased from marine product market, Qingdao, first with flowing water, fish body body surface is washed down, scale, after dry, cut 5cm at fish dorsal along vertebra with sterilizing cutter, cut two ends two dorsal muscles are opened to both sides respectively, then grind in mortar with the appropriate meat tissue of sterile scissors clip.Take 2g and be organized in sterilized empty bottle, add 18ml disinfectant (control group adds the physiological saline of equivalent) and be placed on oscillator it is mixed.Act on respectively to stipulated time (5min, 15min, 30min), get in the neutralizer solution that 0.5ml aforesaid liquid adds 4.5ml sterilizing and mix.After neutralization 10min, get this liquid of 0.1ml and be inoculated in ordinary nutrient agar medium, be placed in after 30 ℃ of incubators are cultivated 48h and make count plate, detect its sterilizing rate.
2.9 acute toxicity experience
2.9.1 to select body weight be 20 of the Kunming mouses of about 30g in the preparation of animal, male and female half and half.Overnight fasting, not water restriction before test.
2.9.2 tested material preparation takes Chinese herbal medicine adding distil water in No. 5 sanitizer formulations ratios and is made into test disinfectant, for subsequent use; LD
50be greater than the true border of 5000mg/kg (body weight) nontoxic.
2.9.3 contamination method gives animal by per os gavage mode by tested material.By the amount of each 0.3ml/10g (body weight), carry out at twice (middle interval 12h), every mouse is first weighed and fills with the disinfectant of respective amount again.After contamination, observe poisoning manifestations and death toll and death time of animal, observing time 14d.
2.10 mouse marrow cell micro nuclear test
2.10.1 the processing of laboratory animal
Selecting body weight is 30 of the Kunming mouses of about 20g, is divided at random three groups, 10 every group, and male and female half and half.Preparation is with the disinfectant of above-mentioned toxicity test same concentrations, and positive controls is selected cyclophosphamide (40mg/kg body weight), and negative control group is selected physiological saline.Animal contaminated adopts per os gavage 30h contamination method, i.e. twice contamination interval 24h, and after contamination, 6h draws materials for the second time.Put to death mouse with cervical vertebra dislocation method, then cut off skin and the muscle on thigh with scissors, take out femur, rub back and forth the muscle disintegrating slag being totally attached on bone with a fritter gauze with the hands.Cut the ball and socket joint that expand at femur two ends, then draw 5ml physiological saline with syringe, insert femur one end, bone marrow cell is rinsed to the centrifuge tube of 10ml.Can repeat to rinse repeatedly, until ossis is white in color.
2.10.2 bone marrow cell processing
Obtained cell suspending liquid, with the centrifugal 10min of 1000rpm, is sucked to supernatant, in sediment, add 2 physiological saline, make cell suspension.Drip suspension in slide one end, smear according to a conventional method dries in air.By dry smear, in the mixture of methyl alcohol and glacial acetic acid, fix 10min.With Ji's nurse Sa application liquid dyeing 10min～15min, then rinse with flowing water, dry.The same experimental group of operation sequence of the positive and negative control group.Select even, complete, the painted suitable region of cell distribution.Under oily mirror, counting is containing polychromatic erythrocyte (PCE) number of micronucleus.PCE is dusty blue [mature erythrocyte (NCE) pinkiness], and how rounded micronucleus is, the smooth of the edge, neat, has a liking for look consistent with karyocyte caryoplasm, is aubergine or bluish violet.Typically have a diameter from erythrocytic 1/20～1/5.1000 PCE of every animal counting.Micronucleus cell rate refers to the PCE number that contains micronucleus, represents with permillage.In 1 PCE, have 2 or multiple micronucleus, still by 1 counting.
2.11 statistical procedures
Application SPSS 19.0 statistical softwares, carry out data processing to above result of the test.Suspension quantitative disinfecting test, simple bactericidal assay and organic matter, the affect result of the test of pH on compound Chinese herb sanitizer disinfecting effect, all adopt one-way analysis of variance to carry out statistical disposition; Stability test, mouse marrow cell micro nuclear test adopt paired t-test to carry out statistical disposition.Inspection level is α=0.05.
3 experimental results
3.1 neutralizer approval tests
Result of the test shows, neutralizer containing lecithin 2.5g/L, sodium thiosulfate 10.0g/L, Tween-80 7.5g/L can effectively neutralize the residual action of the highest experimental concentration compound Chinese herb disinfectant to bacterium, and neutralizer has no significant effect (table 3) to test organisms.
Table 3 neutralizer authentication test results
Note: test temperature is 20 ℃, result is the mean value of 3 tests.
3.2 suspension quantitative disinfecting tests
Result shows, No. 5 disinfectants, to staphylococcus aureus effect 5min, are killed logarithm value and can be reached 5, and the bactericidal effect difference of No. 5 disinfectants and 1～No. 4 disinfectant has statistical significance (P < 0.05), the results are shown in Table 4.1～No. 5 disinfectant is killed logarithm value to Escherichia coli effect 5min, 10min, 15min and is not all reached 5, but No. 5 disinfectants have reached more than 90% to colibacillary killing rate, the results are shown in Table 5,6.Compound Chinese herb disinfectant is killed logarithm value to vibrio parahemolyticus and all reach 5 except No. 1 disinfectant, the results are shown in Table 7.
The killing effect of table 4 variable concentrations compound Chinese herb disinfectant to staphylococcus aureus
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Each concentration disinfectant sterilization 5min, 10min, 15min, F value be respectively 87.04,37.78 and 38.59, P value be all less than 0.05.
Table 5 variable concentrations compound Chinese herb disinfectant is to colibacillary killing effect
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Each concentration disinfectant sterilization 5min, 10min, F value is respectively 5.87,3.62, and P value is all less than 0.05, each concentration disinfectant sterilization 15min, F value is that 3.09, P value is greater than 0.05.
The average kill ratio (%) of table 6 variable concentrations disinfectant to Escherichia coli effect different time
The killing effect of table 7 variable concentrations compound Chinese herb disinfectant to vibrio parahemolyticus
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Each concentration disinfectant sterilization 5min, F value is respectively 32.84, P value and is less than 0.05, each concentration disinfectant sterilization 10min, 15min, F value is 1.0, P value and is all greater than 0.05.
3.3 simple bactericidal assay results
Result shows, No. 5 compound Chinese herb disinfectants are to staphylococcus aureus effect 5min, kill logarithm value and can reach 5, and Andrographis Paniculata, Honegsukle flower P.E, isatis root extract, chitosan hydrochloride respectively the logarithm value of killing when independent role reach far away 5 (in table 8).Statistical analysis, the bactericidal effect difference of No. 5 compound Chinese herb disinfectants and each simple has statistical significance (P < 0.05, table 9), this show compound Chinese herb sanitizer disinfecting effect after compatibility wherein the bactericidal effect of simple composition have significantly and strengthen.
No. 5 compound Chinese herb disinfectants of table 8 and the wherein Comparison of effect on sterilization of simple
Note: test temperature is 20 ℃, result is the mean+SD of 3 tests.
No. 5 compound Chinese herb disinfectants of table 9 and the wherein entirety comparison of simple bactericidal effect
Note: significance is that 0.05, * represents P < 0.05, and the difference between entirety has statistical significance.
3.4 organic substance influence tests
Get test organisms suspension, add a certain proportion of aseptic calf serum, by suspension quantitative disinfecting test program, detect its killing effect to staphylococcus aureus, Escherichia coli, vibrio parahemolyticus.
Staphylococcus aureus and Escherichia coli are chosen disinfectant No. 5, within 1: 1 and 3: 1, mix with volume ratio with calf serum, bactericidal action 5min, result shows, raise with calf serum ratio, kill logarithm value and reduce, and difference there is statistical significance (P < 0.05); Vibrio parahemolyticus is chosen disinfectant No. 5, within 1: 1 and 3: 1, mixes with volume ratio with calf serum, and bactericidal action 10min, bactericidal effect is not subject to the impact of calf serum concentration, the results are shown in Table 10.
The impact of table 10 organic matter on compound Chinese herb sanitizer disinfecting effect
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Staphylococcus aureus F=12.71, P < 0.05; Escherichia coli F=7.30, P < 0.05; Vibrio parahemolyticus F=1.00, P > 0.05.
3.5pH affects result of the test
Get and prepare and through boiling cooling disinfectant solution, measure after its pH value, drip respectively hydrochloric acid (HCl) solution and sodium hydroxide (NaOH) solution, regulate former disinfectant solution pH value to pH-2 and pH+2, press suspension quantitative disinfecting test program, detect its killing effect to staphylococcus aureus, Escherichia coli, vibrio parahemolyticus.
Choose disinfectant No. 5, pH value is 5.0 after measured, adds respectively HCl solution and NaOH solution and regulates former disinfectant solution pH value to 3.0 and 7.0, acts on minimum effective time of 5min.Result shows, disinfectant solution is in the time of acidity or alkali condition, killing logarithm value and all can significantly decline Staphylococcus aureus, difference has statistical significance (P < 0.05), and disinfectant solution sterilization logarithm value to Escherichia coli and vibrio parahemolyticus in the time of acidity or alkali condition has no significant effect, the results are shown in Table 11.In former compound Chinese herb disinfectant, because containing the shitosan that is not soluble in alkaline solution, while adding NaOH solution, can produce yellow grumeleuse, disinfectant proterties changes.
The impact of table 11pH on compound Chinese herb sanitizer disinfecting effect
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Staphylococcus aureus F=198.06, P < 0.05, Escherichia coli F=3.36, P > 0.05.
3.6 stability test
To prepare and pack through boiling No. 1 cooling disinfectant solution, put 54 ℃ of insulating boxs and deposit 14d, depositing forward and backwardly by suspension quantitative disinfecting test program respectively, detecting its killing effect to staphylococcus aureus, Escherichia coli, vibrio parahemolyticus.Result shows, the bactericidal effect basic indifference of disinfectant before and after depositing, the results are shown in Table 12.
Table 12 is deposited front and back compound Chinese herb sanitizer disinfecting effect
Note: test temperature is 20 ℃, result is the mean value of 3 tests.Staphylococcus aureus: t=0.089, P > 0.05; Escherichia coli: t=2.075, P > 0.05; Vibrio parahemolyticus: t=-1.0, P > 0.05.
3.7 marine product bactericidal assay
Scallop: the sterilizing rate that variable concentrations proportioning disinfectant acts on scallop different time all reaches more than 90% (table 13); When disinfectant and scallop effect same time, killing rate increases with concentration, and the sterilizing rate of No. 5 disinfectants is the highest, reaches 100%.
The killing effect of the disinfectant of table 13 variable concentrations proportioning to bacterium in scallop
Note: test temperature is 20 ℃, result is the mean value of 3 tests.
Oyster: result of the test shows, the sterilizing rate that the disinfectant of variable concentrations proportioning acts on oyster different time is substantially more than 85% (table 14), and bactericidal effect is better.
The disinfectant of table 14 variable concentrations proportioning acts on the bactericidal effect of oyster
Note: test temperature is 20 ℃, result is the mean value of 3 tests.
Yellow croaker, flower tongue fish: result of the test shows, the disinfectant of variable concentrations proportioning acts on all more than 99% (table 15,16) of sterilizing rate of yellow croaker different time, and bactericidal effect is fine.
The disinfectant of table 15 variable concentrations proportioning acts on the bactericidal effect of yellow croaker
Note: test temperature is 20 ℃, result is the mean value of 3 tests.
The disinfectant of table 16 variable concentrations proportioning acts on the bactericidal effect of colored tongue fish
Note: test temperature is 20 ℃, result is the mean value of 3 tests.
3.8 acute toxicity experience
After administration 14d, animal has no obvious poisoning symptom, and viewing duration, without animal dead (table 17), can judge that this disinfectant is to chmice acute per os gavage LD
50be greater than 5000mg/kg (body weight), belong to actual nontoxic level material.
Body weight, perfusion amount and the death condition of table 17 test mice
3.9 mouse marrow cell micro nuclear test
Every mouse of experimental group, negative control group and positive controls is counted respectively to 1000 polychromatic erythrocytes (PCE), record its PCE number and micronucleus cell rate (table 18～21) containing micronucleus.Micronucleus cell is taken the lead in carrying out square root data transaction (table 22), then do the t check of two independent sample data.Statistics (table 23) demonstration, experimental group and negative control group be p > 0.05 relatively, there was no significant difference; Experimental group and positive controls be p < 0.05 relatively, has significant difference, illustrate this compound Chinese herb disinfectant to mouse without chromosome damage effect in body.
In table 18 experimental group mouse bone marrow cells, contain polychromatic erythrocyte number and the micronucleus cell rate thereof of micronucleus
In table 19 negative control group mouse bone marrow cells, contain polychromatic erythrocyte number and the micronucleus cell rate thereof of micronucleus
In table 20 positive controls mouse bone marrow cells, contain polychromatic erythrocyte number and the micronucleus cell rate thereof of micronucleus
Table 21 compound Chinese herb disinfectant is to PCEMNR micronucleus test result
Table 22 PCEMNR micronuclear rates descriptive statistics
Note: in table, numerical value is that micronuclear rates carries out the result after square root data transaction.
Table 23 PCEMNR micronucleus test statistical disposition result
Note: significance is that 0.05, * represents P < 0.05, and the difference of this group comparison has statistical significance.
This result of study shows, this compound Chinese herb disinfectant of certain formula rate is better to the killing effect of staphylococcus aureus and vibrio parahemolyticus, although can cause that the bacterium of food poisoning is a lot, the present invention has selected three kinds of Bacteria Indicators-staphylococcus aureuses, Escherichia coli and vibrio parahemolyticus as representing that bacterium has carried out bactericidal assay, and other resistance that can cause food poisoning bacterium is basic suitable with above Three Represents bacterium.In the present invention, disinfectant used is very high to the killing rate of natural bacteria on marine product, proves that this compound Chinese herb disinfectant can meet the requirement of sterilization in daily practical application completely; Acute toxicity experience proves, this compound Chinese herb disinfectant belongs to actual nontoxic level material; Mouse marrow cell micro nuclear test shows, this compound Chinese herb disinfectant hereditary-less toxicity, can relievedly use.
The using method of this disinfectant is:
Product forms of the present invention can be made tablet, if make tablet by the proportioning of formula 5, every containing Andrographis Paniculata 0.1g, Honegsukle flower P.E 0.1g, isatis root extract 0.1g, chitosan hydrochloride 2.0g.The method using is: by soluble in water No. 5 tablets made of formula, every is dissolved in 100ml water, and this is the highest concentration, and soak time is at 5-15 minute.While dilution as needs, can add water, as the every 200ml that adds water, its concentration is about disinfectant No. 3 again, and every is about disinfectant No. 1 while adding water 400ml, also can be according to preparation voluntarily in various extract defined amount ranges.
Claims (3)
1. the compound Chinese herb disinfectant for tableware and kitchen tools sterilization, it is characterized in that: it contains following component and weight consists of: 0.025～0.100 part of Andrographis Paniculata, 0.025～0.100 part of Honegsukle flower P.E, 0.025～0.100 part of isatis root extract, 0.032～2.000 part of chitosan hydrochloride.
2. a kind of compound Chinese herb disinfectant for tableware and kitchen tools sterilization according to claim 1, it is characterized in that: described contains in component, its weight consists of: 0.100 part of Andrographis Paniculata, 0.100 part of Honegsukle flower P.E, 0.100 part of isatis root extract, 2.000 parts of chitosan hydrochlorides.
3. a kind of compound Chinese herb disinfectant for sterilizing dinnerware, kitchen tools according to claim 1 and 2, is characterized in that: the formulation of disinfectant is tablet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210435974.3A CN103783089B (en) | 2012-11-05 | 2012-11-05 | Compound Chinese herbal medicine disinfectant for tableware and kitchenware disinfection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210435974.3A CN103783089B (en) | 2012-11-05 | 2012-11-05 | Compound Chinese herbal medicine disinfectant for tableware and kitchenware disinfection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103783089A true CN103783089A (en) | 2014-05-14 |
| CN103783089B CN103783089B (en) | 2015-05-06 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107624826A (en) * | 2017-11-03 | 2018-01-26 | 纪宏 | A kind of air bacteriostatic agent and preparation method thereof |
| CN108566958A (en) * | 2018-06-11 | 2018-09-25 | 佛山皖阳生物科技有限公司 | A kind of complex biological disinfectant and preparation method thereof |
| CN112400905A (en) * | 2020-12-11 | 2021-02-26 | 陕西汤普医疗器械有限公司 | Acidic oxidation potential water antibacterial liquid and preparation method thereof |
| CN112704088A (en) * | 2020-12-29 | 2021-04-27 | 南京启佑生物科技有限公司 | Special disinfectant for chopping board and preparation method and application thereof |
| CN114027303A (en) * | 2021-11-22 | 2022-02-11 | 辽宁农业职业技术学院 | Edible disinfectant for public environment and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101496530A (en) * | 2009-02-27 | 2009-08-05 | 深圳清华大学研究院 | Composite disinfecting effervescent tablet and preparation method thereof |
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| CN101496530A (en) * | 2009-02-27 | 2009-08-05 | 深圳清华大学研究院 | Composite disinfecting effervescent tablet and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107624826A (en) * | 2017-11-03 | 2018-01-26 | 纪宏 | A kind of air bacteriostatic agent and preparation method thereof |
| CN108566958A (en) * | 2018-06-11 | 2018-09-25 | 佛山皖阳生物科技有限公司 | A kind of complex biological disinfectant and preparation method thereof |
| CN112400905A (en) * | 2020-12-11 | 2021-02-26 | 陕西汤普医疗器械有限公司 | Acidic oxidation potential water antibacterial liquid and preparation method thereof |
| CN112704088A (en) * | 2020-12-29 | 2021-04-27 | 南京启佑生物科技有限公司 | Special disinfectant for chopping board and preparation method and application thereof |
| CN114027303A (en) * | 2021-11-22 | 2022-02-11 | 辽宁农业职业技术学院 | Edible disinfectant for public environment and preparation method and application thereof |
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| CN103783089B (en) | 2015-05-06 |
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