CN103655987A - Special antibacterial agent for raising river crab - Google Patents

Abstract

The invention relates to a special antibacterial agent for raising river crab. The special antibacterial agent is characterized by being prepared by reasonably mixing a plurality of Chinese herbal medicines extracts and then processing according to a special process. The special antibacterial agent is applied to the river crab raising field, can be used for preventing bacterial enteritis, black gill disease, gill rot disease, hydropsy, hepatonecrosis, decladding failure and other river crab diseases, and shows a remarkable effect on exterminating pathogenic microorganism which causes disease, such as vibrio, aeromonas and mould. The special antibacterial agent is the complex antibacterial agent on the basis of the Chinese herbal medicines. The special antibacterial agent has the advantages of being low in concentration, efficient in sterilization, free from medicine irritation and toxic and side effects, natural and harmless.

Description

The special-purpose antibacterial of culture of Chinese mitten crab

technical fieldthe present invention relates to the special-purpose antibacterial of a kind of culture of Chinese mitten crab, it is characterized in that extracting multiple Chinese herbal medicine is that main warp reasonable compatibility forms.The active ingredient of formula is 12 to burn base dimethyl benzene methyl ammonium bromide, hyaluronic acid, chitosan, the Rhizoma Atractylodis Macrocephalae, Rhizoma Acori Calami, Radix Et Rhizoma Rhei, Folium Pyrrosiae, vitamin B12, orotic acid Process configuration and form.This product is applicable to culture of Chinese mitten crab field, prevention enteritis disease, black gill disease disease, gill rot, edema disease, hepatic necrosis disease, the crab disease problem that shells unsuccessful, for the sex pheromone that causes disease, as vibrio, Aeromonas, mycete are all measured obvious killing action.The present invention adopts take Chinese herbal medicine as main composite sterilizing agent, and its feature is to have low concentration, efficient sterilizing, without medicine irritation, has no side effect, natural harmless advantage.

background technologyfrom calendar year 2001, along with the fast development of aquaculture, the innovation of technology, culture of Chinese mitten crab, as one of important topic in special aquaculture technology, has obtained the concern of each layer of society.Pulling up rapidly of culture of Chinese mitten crab industry, area constantly expands, disease problem is also following, be different from raising fish and shrimp sparetime university, crab belongs to Amphibian, and cultivating condition is harsh, water quality and bottom soil property are had to strict requirement, once Infective antibacterial, can cause great loss to raiser, this is also the major reason that has hindered the development of culture of Chinese mitten crab industry.For the harm of antagonism disease, existing employing antibiotic, the halogen chemical agents such as chloride, bromine are prevented and treated, and general early stage, result of use was better, and drug effect can fade away with access times, and main cause is the generation of biology drug resistance.Effects of antibiotics is best, with strong points, after drug resistance occurs, easily causes bacterium living beings structural mutation to produce super bacterium, the loss that its harm super common disorder far away causes.Find after deliberation, Chinese herbal medicine is as a kind of multifunctional drug, there is the clinical application history of more than one thousand years to go through, be applicable to human body and animal body constitution, treatment various diseases is all had to good effect, it is characterized in that thering is broad-spectrum sterilization, no side effects, without drug resistance reaction, can life-time service, the advantages such as medicament sources is abundant, suit the method counteracting each other of nature and organism.The present invention is through screening multiple Chinese herbal medicine, and reasonable composite forming added the crab required nutrient substance of growing, and not only effectively the various antibacterials of control, fungus-caused disease problem, also have various functions such as the immunity of enhancing, digestion promoting, growth promotion.

summary of the inventionmain points of the present invention are to adopt multiple pure natural Chinese herbal medicine and biological preparation is composite forms, according to combination principle, through grinding, 60% ethanol water-bath warm macerating, filters, centrifugal, blend, the techniques such as stirring are made, and have greatly improved the pharmacological action of Chinese herbal medicine, finally make the special-purpose antibacterial of a kind of culture of Chinese mitten crab.

The formula following (component and percentage by weight % thereof) that the present invention selects: 12 burn base dimethyl benzene methyl ammonium bromide 4.0-5.0; Hyaluronic acid 1.5-2.0; Chitosan 3.0-4.0; Rhizoma Atractylodis Macrocephalae 5.0-6.0; Rhizoma Acori Calami 4.0-5.0; Radix Et Rhizoma Rhei 1.5-2.0; Folium Pyrrosiae 3.0-4.0; Vitamin B12 5.0-6.0; Orotic acid 2.5-3.0; Water to 100.0.

12 action principles of burning base dimethyl benzene methyl ammonium bromide are: the 1. ammonium salt class of organic replacement on chemical component, there is 4 substituent groups (R1 ~ R4) alkyl-type heterocyclic group, and one of them long-chain is bactericidal activity group; 2. performance is more stable, and low concentration aqueous solution also can long term storage; 3. all belong to low effect disinfectants, can not killing fungus, tubercule bacillus and antibacterial bud embrace, also can not the strong virus of deactivation drag; 4. bacteriostasis is powerful, and under extremely low concentration, (10-6) still can breed by bacteria growing inhibiting, and its sterilization mechanism is that 1. quaternary ammonium salt molecule can be adsorbed onto thalline surface, changes cell permeability, dissolves damaging cells thalline is broken, the outflow of born of the same parents' content; 2. quaternary ammonium salt molecular surface active function concentrates on thalline surface, hinders bacterial metabolism, makes after birth structure disturbance; 3. be penetrated into and in thalline, make albumen generation degeneration and precipitation; 4. destroy bacterial enzyme system, particularly the activity of dehydrogenase type, oxidases is exerted an influence.

Hyaluronic principle of drug action is one of main matrix composition as epiderm skin and corium; its physiological function is to make moisture enter intercellular substance; and form protein gel with protein bound; cell is sticked together; impelling the homergy effect of cell, participate in the regulation and control that the inside and outside electrolyte of cell exchanges directly, is the physics of cell and the filter of molecular information; keep cell moisture, protective Skin Cell is not subject to the infringement of pathogen.Macromole hyaluronic acid reaches antibacterial effect by controlling movement, propagation, differentiation and the phagocytic function of pathogenic bacteria cell.

The action principle of chitosan is to suppress the growth and breeding of some funguses, antibacterial and virus.Its mechanism has three: one, due to the polycation of chitosan, is easy to the group effect of fungal cell's surface band negative charge, thereby changes mobility and the permeability of pathogen cell membrane; The 2nd, disturb copying and transcribing of DNA; The 3rd, the metabolism of blocking-up pathogen.

Rhizoma Atractylodis Macrocephalae rhizome is containing volatile oil, and infusion has inhibitory action to acrothesium floccosum, star nocardia in vitro.Its decoct also has inhibitory action to meningococcus, and Bacillus typhi, paratyphoid bacillus A, shigella flexneri, escherichia coli, bacillus pyocyaneus etc. are all had to bacteriostasis in various degree.

The action principle of Rhizoma Acori Calami is: this product is containing fragrant volatile oil 1.5～3.0%, is mainly oily capryl alcohol, asarylic acid, methyleugenol, rich terpenes etc. doubly in oil, contains amorphism bitterness.Staphylococcus aureus, Staphylococcus albus, streptococcus pneumoniae, escherichia coli, dysentery bacterium, Bacillus typhi, trichophyton, Trichophyton concentricunm, star-shaped nocardia are also had to inhibitory action in various degree.Can prevent and treat enteritis, red skin, rotten cheek, fish molds etc.

The action principle of Radix Et Rhizoma Rhei: it is strong that it has antibacterial action, has a broad antifungal spectrum; Its effective ingredient is anthraquinone derivative, wherein best with the antibacterial action of chrysophanic acid, emodin and aloe-emodin; There is convergence, increase platelet, promote blood coagulation and antitumor action.Be used for preventing and treating hemorrhagic disease, bacterial gill rot disease, white head and mouth fish disease etc.

In mainly containing in Folium Pyrrosiae from alkene, 8 one sitosterol, chlorogenic acid, the rich fruit glycosides of dying, the different rich Multiple components such as fruit glycosides, Quercetin, isoquercitin, sucrose of dying.The pyrrosia sheareriChing suspension of 5% above concentration has inhibitory action to dysentery bacterium, ileotyphus bacillus, Salmonella paratyphi; Folium Pyrrosiae all has inhibitory action in various degree to staphylococcus aureus, Hemolytic streptococcus, anthrax bacillus, diphtheria corynebacterium, escherichia coli.

The action principle of vitamin B12: its manufacture and renew the erythrocyte in body, can prevent anemia, contribute to growing, the nervous system of keeping fit, subduction allergic conditions, promotes the equilibrant of health.

The action principle of orotic acid: it contributes to the metabolism of vitamin B, can together carry out metabolism with vitamin B12 and folic acid, very important to the recovery of cell and repairing.

The preparation method of product of the present invention is:

1) Rhizoma Atractylodis Macrocephalae, Rhizoma Acori Calami, Radix Et Rhizoma Rhei, Folium Pyrrosiae are through grinding;

2) 60% alcoholic solution thermostat water bath warm macerating 1 ~ 2h, filters;

3) Chinese herbal medicine fluid leaching is mixed, 2000 revs/min are carried out centrifugally, extract the medicinal liquid of supernatant;

4) by 12 burn base dimethyl benzene methyl ammonium bromide, hyaluronic acid, chitosan, vitamin B12, orotic acid be mixed in proportion be added to extract supernatant medicinal liquid in reactor, be uniformly mixed;

5) with sterile distilled water, be settled to desired concn, low temperature is sealed up for safekeeping standby.

The present invention has the following advantages:

(1) selected natural high-quality Chinese herbal medicine refines, nontoxic residue-free;

(2) Chinese herbal medicine antibacterial, sterilization that screens multiple epistasis carries out compatibility, has the effect of high-efficiency broad spectrum sterilization;

(3) nonirritant reaction, free from extraneous odour;

(4) can improve immune effect;

(5) the experiment proved that, active ingredient is stable, antimicrobial long-acting, long shelf-life.

the specific embodimentbelow in conjunction with embodiment, the present invention is described in further detail:

According to following table institute column data (percentage by weight %) and described step thereof, be configured:

Formula one:

12 burn base dimethyl benzene methyl ammonium bromide 4.0-7.0

Hyaluronic acid 1.5-4.0

Chitosan 3.0-6.0

Rhizoma Atractylodis Macrocephalae 5.0-8.0

Rhizoma Acori Calami 4.0-9.0

Radix Et Rhizoma Rhei 1.5-5.0

Folium Pyrrosiae 3.0-8.0

Vitamin B12 2.0-4.0

Orotic acid 2.5-4.0

Water to 100.0

Formula two:

12 burn base dimethyl benzene methyl ammonium bromide 5.0-7.0

Hyaluronic acid 1.5-3.0

Chitosan 4.0-6.0

Rhizoma Atractylodis Macrocephalae 5.0-7.0

Rhizoma Acori Calami 5.0-9.0

Radix Et Rhizoma Rhei 1.5-4.0

Folium Pyrrosiae 4.0-8.0

Vitamin B12 2.0-3.0

Orotic acid 3.5-4.0

Water to 100.0

________________________________________________________________________

Formula three:

12 burn base dimethyl benzene methyl ammonium bromide 5.0-6.0

Hyaluronic acid 2.5-3.0

Chitosan 4.0-5.0

Rhizoma Atractylodis Macrocephalae 6.0-7.0

Rhizoma Acori Calami 5.0-8.0

Radix Et Rhizoma Rhei 2.5-4.0

Folium Pyrrosiae 4.0-7.0

Vitamin B12 2.5-3.0

Orotic acid 2.5-4.0

Water to 100.0

Claims (2)

1. the present invention relates to the special-purpose antibacterial of a kind of culture of Chinese mitten crab, it is characterized in that having following formula (component and percentage by weight % thereof): 12 burn base dimethyl benzene methyl ammonium bromide 4.0-5.0; Hyaluronic acid 1.5-2.0; Chitosan 3.0-4.0; Rhizoma Atractylodis Macrocephalae 5.0-6.0; Rhizoma Acori Calami 4.0-5.0; Radix Et Rhizoma Rhei 1.5-2.0; Folium Pyrrosiae 3.0-4.0; Vitamin B12 5.0-6.0; Orotic acid 2.5-3.0; Water to 100.0.

2. a preparation method for the special-purpose antibacterial of culture of Chinese mitten crab described in claim 1, is characterized in that having following step:

1) Rhizoma Atractylodis Macrocephalae, Rhizoma Acori Calami, Radix Et Rhizoma Rhei, Folium Pyrrosiae are through grinding;

2) 60% alcoholic solution thermostat water bath warm macerating 1 ~ 2h, filters;

3) Chinese herbal medicine fluid leaching is mixed, 2000 revs/min are carried out centrifugally, extract the medicinal liquid of supernatant;

4) by 12 burn base dimethyl benzene methyl ammonium bromide, hyaluronic acid, chitosan, vitamin B12, orotic acid be mixed in proportion be added to extract supernatant medicinal liquid in reactor, be uniformly mixed;

5) with sterile distilled water, be settled to desired concn, low temperature is sealed up for safekeeping standby.