CN114081903A - Plant extract with antibacterial effect, and extraction method and application thereof - Google Patents
Plant extract with antibacterial effect, and extraction method and application thereof Download PDFInfo
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- 238000000605 extraction Methods 0.000 title claims abstract description 17
- 230000000844 anti-bacterial effect Effects 0.000 title description 6
- 241000219195 Arabidopsis thaliana Species 0.000 claims abstract description 21
- 208000004396 mastitis Diseases 0.000 claims abstract description 17
- 238000010992 reflux Methods 0.000 claims abstract description 13
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- 241000219194 Arabidopsis Species 0.000 claims description 16
- 235000013365 dairy product Nutrition 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 9
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- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 claims description 3
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The invention discloses a plant extract with bacteriostatic action, an extraction method and application thereof, wherein the plant extract is an ethanol extract of arabidopsis thaliana; the extraction method is a reflux extraction method or an ultrasonic extraction method; solves the problems of drug resistance generation, drug residue and the like caused by using antibiotics to prevent and treat the cow mastitis at present.
Description
Technical Field
The invention relates to a plant extract and application of the plant extract in preventing and treating cow mastitis.
Background
Oxidative stress is liable to cause mastitis, ketosis, etc. in dairy cows. The mastitis of the dairy cow is one of the most common and important diseases for restricting the development of the dairy cow breeding industry, not only affects the reduction of milk yield and the reduction of milk quality, but also even leads the dairy cow to lose production performance and causes serious economic loss, and in addition, the mastitis is treated, and the problems of medicament residue and the like are caused by using a large amount of antibiotics, thereby seriously harming human health. It is reported that about 2.2 million cows are stocked globally and about 1/3 cows have each type of mastitis, causing economic losses of up to $ 350 million; more than 1000 million cows are kept in a stall in China, the incidence rate of clinical mastitis is about 30 percent, and the incidence rate of recessive mastitis reaches 70 percent.
Antibiotics are listed as the first choice drugs for treating mastitis all over the world for decades, however, due to long-term repeated use, pathogens generate drug resistance to drugs, the curative effect is reduced, and the residue of antibiotics in milk is increased, so that the development and utilization of antibiotics substitutes becomes a current research hotspot.
The technology for preventing and treating mastitis by adding the plant extract into daily ration of the dairy cow is a hot point of scientific research in recent years, the plant extract is a natural plant component, has no toxic action on animals, and has a very wide application prospect, so that the screening of the plant extract suitable for preventing and treating the mastitis of the dairy cow has important significance.
Disclosure of Invention
In view of the above, the present invention provides a plant extract with bacteriostatic effect.
The invention also provides a preparation method of the plant extract and a dairy cow feed additive using the plant extract
In a first aspect, the plant extract with bacteriostatic action is characterized in that:
the plant is a Arabidopsis thaliana.
Further, the Arabidopsis was extracted by ethanol.
In a second aspect, a method for extracting a plant extract, comprising:
a mixture of Arabidopsis thaliana and ethanol;
subjecting the mixture to heat reflux or sonication to obtain an ethanol extract of Arabidopsis thaliana.
Further, freeze-drying the ethanol extract of Arabidopsis thaliana to obtain the ethanol extract of Arabidopsis thaliana powder.
Further, the preparation method of the mixture comprises the following steps:
pulverizing said Arabidopsis followed by adding said ethanol to obtain said mixture;
the mass ratio of the said mustard and the said ethanol is 1: 7 or 1: 42.
Further, the temperature of the heating reflux is 75 ℃, and the reflux time is 120 minutes.
Further, the sonication time was 40 minutes.
In a third aspect, the application of a plant extract in preparing a medicament or feed for preventing and treating cow mastitis is provided.
In a fourth aspect, a dairy cow feed additive, comprising:
the plant extract of the first aspect.
Further, the dairy cow feed additive consists of an ethanol extract of the Arabidopsis thaliana.
The invention has the following beneficial effects:
the active ingredients of the wasabi extract mainly comprise polyphenol, flavone, alkaloid and the like, and the wasabi extract has strong antibacterial and antioxidant effects, so that staphylococcus aureus causing cow mastitis can be effectively inhibited, and cow mastitis can be effectively prevented and controlled; the feed additive for the dairy cattle contains the Thellungiella salsuginea extract, so the feed additive is rich in chemical active substances, has the effects of bacteriostasis, anti-inflammation, heat clearing and detoxifying and the like, accords with the policies of 'banning' at the feed end and 'reducing and limiting' at the breeding end proposed by the country, is beneficial to preventing and controlling the mastitis of the dairy cattle, improves the milk yield, reduces the generation of antibiotic residues and drug resistance in the milk, and protects the health of people.
Drawings
The above and other objects, features and advantages of the present invention will become more apparent from the following description of the embodiments of the present invention with reference to the accompanying drawings, in which:
FIG. 1 is a graph of the inhibitory effect of the Arabidopsis extract SJ-1 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 2 is a graph of the inhibitory effect of the Arabidopsis extract SJ-2 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 3 is a graph of the inhibitory effect of the Arabidopsis extract SJ-3 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 4 is a graph of the inhibitory effect of the Arabidopsis extract SJ-4 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 5 is a graph of the inhibitory effect of the Arabidopsis extract SJ-5 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 6 is a graph of the inhibitory effect of the Arabidopsis extract SJ-6 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 7 is a graph of the inhibitory effect of the Arabidopsis extract SJ-7 of the examples of the present disclosure on Staphylococcus aureus;
FIG. 8 is a graph of the inhibitory effect of the Arabidopsis extract SJ-8 of the examples of the present disclosure on Staphylococcus aureus.
Detailed Description
The present invention will be described below based on examples, but it should be noted that the present invention is not limited to these examples. In the following detailed description of the present invention, certain specific details are set forth. However, the present invention may be fully understood by those skilled in the art for those parts not described in detail.
Furthermore, those skilled in the art will appreciate that the drawings are provided solely for the purposes of illustrating the invention, features and advantages thereof, and are not necessarily drawn to scale.
Also, unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is, the meaning of "includes but is not limited to".
The arabidopsis thaliana, also known as goat arabidopsis thaliana, radishes, sauerkraut, sandcap and Mongolian name 'Menglason-Lao pump', is a big annual or biennial herb of brassicaceae and arabidopsis, and the plant height is 0.5-2 m. The arabidopsis thaliana is a special plant of Mongolian plateau desert and sand land, and is grown on the sand land or a semi-fixed and mobile sand dune in a grassland area. The arabidopsis thaliana is mainly distributed in inner Mongolia, Shaanxi, Ningxia, Gansu and other places, has higher nutritional value and health care value, and is in good standing of desert ginseng. The wasabi has fresh and unique mustard flavor, high nutrition and health care value, and is rich in various vitamins, trace elements and crude fibers. The leaf of Thellungiella salsuginea has strong antibacterial effect, and the active ingredients of the leaf mainly comprise polyphenol, flavone, alkaloid and the like.
The invention is based on that the Arabidopsis contains a plurality of active ingredients and has strong bacteriostatic and antioxidant effects, so the research and establishment of the extraction process conditions of the Arabidopsis active ingredients provide a specific technical scheme for preventing and controlling the cow mastitis.
Therefore, the present disclosure describes specific technical solutions adopted to implement the above inventive concepts in combination with specific embodiments.
The first embodiment is as follows: ethanol reflux extraction of Thellungiella salsuginea
Drying caulis et folium Brassicae Junceae, pulverizing, mixing with ethanol, heating the mixture to 75 deg.C, refluxing for 120 min for extraction, recovering ethanol, and drying the extractive solution by freeze-drying machine to obtain powder.
TABLE 1 Thellugium sinense ethanol reflux extraction experimental data
Example two: ultrasonic extraction of Thellungiella salsuginea with ethanol
Drying caulis et folium Brassicae Junceae, pulverizing, mixing with ethanol, performing ultrasonic treatment for 40 min to obtain extractive solution, filtering, and drying the filtrate with a freeze drier to obtain powder.
TABLE 2 ultrasonic extraction of Arabidopsis ethanol Experimental data
Experimental example: bacteriostasis test
The experimental example disclosed herein adopts an oxford cup method for carrying out a bacteriostatic test, and the specific experimental method is as follows:
80g of the ethanol extract powder of the Arabidopsis thaliana obtained in the first and second examples are respectively weighed and dissolved in ultrapure water to prepare a 1g/mL solution of the Arabidopsis thaliana extract, and the solution is sterilized at 121 ℃ for 20min under ultra-high pressure and then placed at 4 ℃ for later use.
The strain placed at-80 ℃ was thawed and cultured in nutrient broth with shaking at 37 ℃ for 18 h. And (4) marking the activated bacterial liquid on a nutrient agar culture medium, and standing and culturing at 37 ℃ for 24 h. And selecting the purified single strain, inoculating the single strain into a nutrient broth culture medium, and performing shaking culture at 37 ℃ for 18h to obtain a raw bacterium solution. The concentration of staphylococcus aureus liquid is adjusted to 1 × 106CFU/mL by using sterilized normal saline, and the staphylococcus aureus liquid is used as test liquid for standby.
Transferring 0.1mL of prepared test bacterial liquid of staphylococcus aureus to a prepared nutrient agar plate, uniformly coating the bacterial liquid by using an aseptic coating rod, drying the bacterial liquid, placing sterilized oxford cups in the plate by using a pair of tweezers, slightly pressurizing the plate to enable the plate to be in seamless contact with a culture medium, placing 4 oxford cups in each plate, adding the same abrotanum extract into 3 oxford cups to serve as a test group, adding the nutrient broth with the same volume in the other oxford cup to serve as a negative control, and simultaneously using norfloxacin as a positive control. And (3) placing the flat plate in an incubator at 37 ℃ for static culture for 24h, measuring the bacteriostatic diameter of the flat plate by using a vernier caliper according to a cross method, and judging the bacteriostatic effect.
The judgment standard of the antibacterial effect is according to the standard of traditional Chinese medicine pharmacology, namely: the bacteriostatic diameter is more than or equal to 20mm, the hypersensitivity is 15-19mm, the medium hypersensitivity is 10-14mm, and the hypohypersensitivity is below 10 mm.
TABLE 3 inhibitory Effect of Arabidopsis thaliana extract on Staphylococcus aureus
Note: indicates high sensitivity (15-19mm), medium sensitivity (10-14mm), low sensitivity (less than 10mm), no inhibitory effect
As can be seen from tables 1, 2, 3 and figures 1-8, the four ultrasonic extraction methods of the arabidopsis thaliana have an inhibiting effect on staphylococcus aureus, and only the first two extraction methods of the reflux extraction method have an antibacterial effect, which indicates that the ultrasonic extraction method is superior to the reflux extraction method; and the reflux extraction methods SJ-1 and SJ-2 and the ultrasonic extraction methods SJ-5 and SJ-7 are superior to other methods.
The above-mentioned embodiments are merely embodiments for expressing the invention, and the description is specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, various changes, substitutions of equivalents, improvements and the like can be made without departing from the spirit of the invention, and these are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A plant extract with bacteriostasis function is characterized in that:
the plant is a Arabidopsis thaliana.
2. The plant extract as claimed in claim 1, wherein:
the Arabidopsis is extracted by ethanol.
3. A method for extracting a plant extract, comprising:
a mixture of Arabidopsis thaliana and ethanol;
subjecting the mixture to heat reflux or sonication to obtain an ethanol extract of Arabidopsis thaliana.
4. The extraction method according to claim 3, characterized in that:
freeze drying the ethanol extract of Arabidopsis thaliana to obtain the ethanol extract of Arabidopsis thaliana powder.
5. The extraction process according to claim 3, characterized in that the mixture is prepared by:
pulverizing said Arabidopsis followed by adding said ethanol to obtain said mixture;
the mass ratio of the said mustard and the said ethanol is 1: 7 or 1: 42.
6. The extraction method according to claim 5, characterized in that:
the heating reflux temperature is 75 ℃, and the reflux time is 120 minutes.
7. The extraction method according to claim 5, characterized in that:
the sonication time was 40 minutes.
8. An application of plant extract in preparing medicine or feed for preventing and treating cow mastitis is provided.
9. A dairy cow feed additive, comprising:
the plant extract of claim 1 or 2.
10. The dairy cow feed additive of claim 9 consisting of an ethanol extract of Arabidopsis thaliana.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588551A (en) * | 2018-11-21 | 2019-04-09 | 南宁市黄陈生猪养殖场 | A kind of feed addictive and preparation method thereof for treating metabolic disease |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588551A (en) * | 2018-11-21 | 2019-04-09 | 南宁市黄陈生猪养殖场 | A kind of feed addictive and preparation method thereof for treating metabolic disease |
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| Title |
|---|
| 乌仁格格等: "乌拉特前旗地区沙芥总黄酮提取方法对比研究", 《化工管理》 * |
| 宋洁等: "植物提取物对奶牛乳房炎的影响及作用机制", 《动物营养学报》 * |
| 张凤兰等: "沙芥果实精油提取工艺优化及GC-MS分析", 《食品研究与开发》 * |
| 薛焕焕: "沙芥营养成分和沙芥多酚的提取、纯化及其生物活性研究", 《中国优秀博硕士学位论文全文数据库（硕士） 工程科技I辑》 * |
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