CN112516276A

CN112516276A - Traditional Chinese medicine for preventing novel coronavirus pneumonia, preparation process thereof and traditional Chinese medicine beverage

Info

Publication number: CN112516276A
Application number: CN202011102715.XA
Authority: CN
Inventors: 傅正美
Original assignee: Hubei Sidakang Traditional Chinese Medicine Technology Co ltd
Current assignee: Hubei Sidakang Traditional Chinese Medicine Technology Co ltd
Priority date: 2020-10-15
Filing date: 2020-10-15
Publication date: 2021-03-19

Abstract

The invention discloses a traditional Chinese medicine for preventing novel coronavirus pneumonia and a preparation process and a traditional Chinese medicine beverage thereof, wherein the raw material components of the traditional Chinese medicine comprise, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng/American ginseng, 1-14 parts of poria cocos, 2-17 parts of radix puerariae, 1-18 parts of dried ginger, 3-16 parts of dried orange peel, 2-13 parts of elsholtzia and 1-8 parts of liquorice. The invention has the effects of tonifying qi and spleen, relieving muscles and relieving exterior syndrome, eliminating dampness and eliminating phlegm and the like, can better regulate the internal and external damp environments of a human body, benefits the transportation and transformation functions of the spleen and the stomach, improves the immunity of the organism and has better prevention effect on the neocoronary pneumonia.

Description

Traditional Chinese medicine for preventing novel coronavirus pneumonia, preparation process thereof and traditional Chinese medicine beverage

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine for preventing novel coronavirus pneumonia, a preparation process thereof and a traditional Chinese medicine beverage.

Background

At present, the clinical treatment of the new coronary pneumonia is mainly performed, the treatment mostly considers corticoids and antibiotics, and the use of chemical drugs can cause a series of adverse reactions in the later period.

On the premise of high importance and scientific prevention and control, the strategic achievement of epidemic prevention and control is obtained by adopting an epidemic prevention and control strategy combining traditional Chinese medicine and western medicine, and the overall situation is controlled to be relatively stable. Basically, the condition that the small-scale prevalence and certain small-scale outbreak occur intermittently is only one. The traditional Chinese medicine 'three medicines and three parties' realizes the full coverage of treatment on patients with mild new coronary pneumonia, common type and severe cases, the total effective rate reaches more than 93%, the traditional Chinese medicine becomes the key for gaining the success of epidemic prevention and control in China, and the traditional Chinese medicine shows the huge achievement of the 'Chinese scheme' of epidemic prevention and control in the world.

Traditional Chinese medicine has thousands of years of experience accumulation in the prevention and treatment of infectious diseases. The new coronary pneumonia belongs to the category of epidemic diseases in the traditional Chinese medicine, and the cause of the new coronary pneumonia is the qi of epidemic disease crime. Traditional Chinese medicine emphasizes that 'treating the disease in the first place and preventing the disease in the first place' and 'paying attention to the prevention of the disease in the first place'. Emphasizes that the 'avoiding of toxic gas' is far away from the infection source and does not touch with the patient and suspected patient, thus avoiding the invasion of toxic gas; meanwhile, the traditional Chinese medicine is also used for regulating the body state of a human body, improving the immunity and resisting viruses.

Therefore, the development of the traditional Chinese medicine beverage for preventing the new coronavirus pneumonia has very important significance.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine for preventing novel coronavirus pneumonia and a preparation process and a traditional Chinese medicine beverage, wherein the traditional Chinese medicine beverage is prepared by scientifically composing nine medicinal materials with homology of medicine and food, such as chrysanthemum, tuckahoe and the like according to the theory of homology of medicine and food of traditional Chinese medicine, combining the clinical medication experience of traditional Chinese medicine and the analysis data of the chemical composition of the traditional Chinese medicine by modern science and technology, and developing the beverage capable of preventing the virus including SARS-CoV-2 and improving the immunity of the organism by adopting the modern preparation technology, and can be immediately marketed.

In order to achieve the purpose, the traditional Chinese medicine for preventing the novel coronavirus pneumonia comprises, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng (artificially planted)/American ginseng, 1-14 parts of poria cocos, 2-17 parts of radix puerariae, 1-18 parts of dried ginger, 3-16 parts of dried orange peel, 2-13 parts of elsholtzia and 1-8 parts of liquorice.

Further, the raw material components of the traditional Chinese medicine comprise, by weight, 15-30 parts of chrysanthemum, 10-20 parts of cinnamon, 8-18 parts of ginseng (artificially planted)/American ginseng, 5-10 parts of poria cocos, 5-15 parts of radix puerariae, 3-15 parts of dried ginger, 5-12 parts of dried orange peel, 5-10 parts of elsholtzia and 3-6 parts of liquorice.

Furthermore, the raw material components of the traditional Chinese medicine comprise, by weight, 20 parts of chrysanthemum, 10 parts of cinnamon, 18 parts of ginseng (artificially planted)/American ginseng, 18 parts of poria cocos, 15 parts of radix puerariae, 6 parts of dried ginger, 12 parts of dried orange peel, 10 parts of elsholtzia and 6 parts of liquorice.

The invention also provides a preparation process of the traditional Chinese medicine for preventing the novel coronavirus pneumonia, which comprises the following steps:

1) comprises chrysanthemum, cinnamon, ginseng (artificially planted)/American ginseng, tuckahoe, kudzu root, dried ginger, dried orange peel, Chinese mosla herb and liquorice in parts by weight; respectively pulverizing into coarse powder;

2) mixing coarse powders of flos Chrysanthemi, cortex Cinnamomi, pericarpium Citri Tangerinae, herba Moslae and Glycyrrhrizae radix, soaking in water, extracting by steam distillation, filtering, separating to obtain volatile oil, distillate and residue, and collecting the volatile oil, distillate and residue respectively; collecting the filtrate in another container;

3) mixing the above residues with Ginseng radix/radix Panacis Quinquefolii coarse powder, radix Puerariae coarse powder, Poria coarse powder and Zingiberis rhizoma coarse powder, adding water, heating, reflux-extracting, filtering to obtain reflux filtrate, mixing the reflux filtrate with the above distilled filtrate, concentrating the combined filtrate under reduced pressure at 50-80 deg.C, and cooling to obtain fluid extract;

4) adding ethanol into the above fluid extract, stirring, standing, collecting supernatant, recovering ethanol, and concentrating the supernatant under reduced pressure at 50-80 deg.C to obtain traditional Chinese medicine.

Preferably, in the step 2), the weight ratio of the mixture of the five crude powder of the chrysanthemum, the cinnamon, the dried orange peel, the elsholtzia herb and the liquorice to the water is 1: 5-10; soaking for 0.5-3h, and extracting for 1.5-5h by steam distillation;

in the step 3), the weight ratio of the mixture of the medicine residues, the ginseng/American ginseng coarse powder, the kudzuvine root coarse powder and the tuckahoe coarse powder to water is 5-10, the extraction time is 2-4h, and the relative density of the clear paste is 1.0-1.3(60 ℃).

Preferably, in the step 4), the volume fraction of ethanol in the mixed solution of the clear paste and the ethanol is 40-70%, the standing time is 12-24 hours, and the relative density of the thick paste is 1.0-1.3(80 ℃).

The invention also provides a traditional Chinese medicine beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of the traditional Chinese medicine and 2-80 parts of auxiliary materials in parts by weight.

Further, the traditional Chinese medicine beverage is a solid beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 2-30 parts of auxiliary materials in parts by weight; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 1: 1-3: 1-3: 4-20: 0.003-0.02;

or the traditional Chinese medicine beverage is concentrated beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 10-80 parts of auxiliary materials in parts by weight; and the adjuvants comprise sucrose/sugar alcohol, water, sweetener, antiseptic, thickener and edible essence; the weight ratio of the sucrose/sugar alcohol, water, the sweetening agent, the preservative, the thickening agent and the edible flavor is 1-5: 10-80: 0-0.08: 0-0.04: 0-1.6: 0-0.08.

Furthermore, the traditional Chinese medicine beverage is a solid beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 5 parts of auxiliary materials in parts by weight; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein, the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetening agent is 2: 1: 2: 0.012.

or the traditional Chinese medicine beverage is concentrated beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 70 parts of auxiliary materials in parts by weight; and the auxiliary materials comprise sugar alcohol, water, a sweetening agent, a preservative, a thickening agent and edible spice; the weight ratio of sugar alcohol, water, sweetener, preservative, thickener and edible flavor is 5: 65: 0.02: 0.015: 0.8: 0.04.

the preparation process of the traditional Chinese medicine beverage comprises the following steps:

1) the traditional Chinese medicine is prepared by the following steps:

a. the traditional Chinese medicine composition comprises, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng (artificially planted), 1-14 parts of poria cocos, 2-17 parts of radix puerariae, 1-18 parts of dried ginger, 3-16 parts of pericarpium citri reticulatae, 2-13 parts of elsholtzia and 1-8 parts of liquorice; respectively pulverizing into coarse powder;

b. mixing coarse powders of flos Chrysanthemi, cortex Cinnamomi, pericarpium Citri Tangerinae, herba Moslae and Glycyrrhrizae radix, soaking in water, extracting by steam distillation, filtering, separating to obtain volatile oil, distillate and residue, and collecting the volatile oil, distillate and residue respectively; collecting the filtrate in another container;

c. mixing the above residues with Ginseng radix coarse powder, radix Puerariae coarse powder, Poria coarse powder and Zingiberis rhizoma coarse powder, adding water, heating, reflux-extracting, filtering to obtain reflux filtrate, mixing the reflux filtrate with the above distillate filtrate, concentrating the combined filtrate under reduced pressure at 50-80 deg.C, and cooling to obtain fluid extract;

d. adding ethanol into the above fluid extract, stirring, standing, collecting supernatant, recovering ethanol, and concentrating the supernatant under reduced pressure at 50-80 deg.C to obtain Chinese medicinal composition;

2) the traditional Chinese medicine beverage is prepared by the following steps:

if the traditional Chinese medicine beverage is a solid beverage,

weighing 1 part of the traditional Chinese medicines and 2-80 parts of auxiliary materials according to the weight part ratio; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 1-3: 1-3: 4-20: 0.003-0.02.

Adding beta-cyclodextrin into the volatile oil obtained in the step for inclusion to obtain an inclusion compound;

thirdly, adding starch, dextrin, a sweetening agent and the inclusion compound into the traditional Chinese medicine, mixing, granulating and drying to obtain the solid beverage;

or, if the traditional Chinese medicine beverage is a concentrated beverage,

i. weighing 1 part of the traditional Chinese medicine and 2-80 parts of auxiliary materials according to the weight part ratio; and the adjuvants comprise sucrose/sugar alcohol, water, sweetener, antiseptic, thickener and edible essence; the weight ratio of the sucrose/sugar alcohol, water, the sweetening agent, the preservative, the thickening agent and the edible flavor is 1-5: 10-80: 0-0.08: 0-0.04: 0-1.6: 0 to 0.08;

adding sucrose/sugar alcohol, water, sweetener, antiseptic, thickener and edible flavor into the Chinese medicinal materials, and mixing to obtain concentrated beverage;

3) and (4) taking the prepared solid beverage or concentrated beverage, packaging, sterilizing, inspecting and warehousing to obtain a finished product of the solid beverage or the concentrated beverage.

The invention adopts the following raw materials:

ginseng: is dried root and rhizome of Panax ginseng C.A.Mey. Sweet in property and slightly bitter in taste. It enters spleen, lung, heart and kidney meridians. Has effects of invigorating primordial qi, recovering pulse, relieving depletion, invigorating spleen, benefiting lung, promoting fluid production, nourishing blood, tranquilizing mind, and improving intelligence. Can be used for treating asthenia, collapse, cold limbs, weak pulse, spleen deficiency, anorexia, lung deficiency, cough, asthma, body fluid deficiency, thirst, internal heat, diabetes, deficiency of both qi and blood, asthenia, palpitation, insomnia, sexual impotence, and cold womb.

Chrysanthemum: is a dried capitula of the Compositae plant Chrysanthemum morifolium ramat. Sweet, bitter and slightly cold in nature. It enters lung and liver meridians. Has the effects of dispelling wind, clearing heat, calming liver, improving eyesight, and clearing away heat and toxic materials. Can be used for treating wind-heat type common cold, headache, giddiness, conjunctival congestion, edema, dim eyesight, and intestinal carbuncle.

Cinnamon: is dried bark of Cinnamomum cassia Cassia Presl of Lauraceae. Pungent and sweet in flavor and large heat. It enters kidney, spleen, heart and liver meridians. Has the effects of supplementing fire and supporting yang, guiding fire to the origin, dispelling cold and relieving pain, and warming channels and promoting blood circulation. Can be used for treating sexual impotence, cold womb, cold pain of waist and knee, asthma due to kidney deficiency, floating of deficient yang, giddiness, conjunctival congestion, cold pain of heart and abdomen, vomiting and diarrhea due to deficiency cold, cold hernia, abdominal pain, dysmenorrhea, and amenorrhea.

Dried ginger: is dried rhizome of Zingiber officinale Rosc. Pungent in nature and hot in nature. It enters spleen, stomach, kidney, heart and lung meridians. Has the effects of warming spleen and stomach for dispelling cold, restoring yang and dredging collaterals, and warming lung and resolving fluid retention. Can be used for treating abdominal psychroalgia, emesis, diarrhea, cold limbs, slight pulse, cough and asthma due to cold fluid retention.

Tuckahoe, poria cocos: the product is dried sclerotium of Wolf of Poria cocos (Schw.) Cos (belonging to Polyporaceae). Sweet, bland and mild in nature. It enters heart, lung, spleen and kidney meridians. Has effects in promoting diuresis, eliminating dampness, invigorating spleen, and calming heart. Can be used for treating edema, oliguria, phlegm and fluid retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.

Dried orange peel: is dried mature pericarp of Citrus reticulata Blanco of Rutaceae and its cultivar. Bitter and pungent in property and warm in nature. It enters lung and spleen meridians. Has the effects of regulating qi, strengthening spleen, eliminating dampness and phlegm. Can be used for treating abdominal distention, anorexia, vomiting, diarrhea, cough, and excessive phlegm.

Herba Moslae: is dried aerial part of Mosla chinensis Maxim or Mosla chinests 'Jiangxiangru' of Labiatae. Pungent in flavor and slightly warm in nature. It enters lung and stomach meridians. Has effects of inducing sweat, relieving exterior syndrome, eliminating dampness, and regulating stomach. Can be used for treating summer-heat and dampness type common cold, aversion to cold, fever, headache, anidrosis, abdominal pain, vomiting, diarrhea, edema, and dysuria.

Licorice root: is dried root and rhizome of Glycyrrhiza glabra Fisch, Glycyrrhiza inflata Gyrhiza inata Bat, or Glycyrrhiza glabra Giyrhia glara L. Sweet in nature and mild in nature. It enters heart, lung, spleen and stomach meridians. Has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, eliminating phlegm and relieving cough, relieving spasm and pain, and harmonizing the medicines. Can be used for treating weakness of spleen and stomach, asthenia, palpitation, short breath, cough, excessive phlegm, abdominal and limb spasm, pain, carbuncle, swelling, sore, and drug toxicity and strong nature.

The formula of the invention combines various medicines, has the effects of tonifying qi and strengthening spleen, relieving muscles and relieving exterior syndrome, eliminating dampness and eliminating phlegm and the like, can better regulate the internal and external damp environments of a human body, benefit the functions of transporting and transforming the spleen and stomach, improve the immunity of the organism and has better prevention effect on the Xinguan pneumonia.

The invention has the beneficial effects that:

in the formula of the invention, the ginseng and the dried ginger are monarch drugs, and the ginseng is sweet and warm and can tonify qi, invigorate spleen and nourish stomach; the dried ginger warms the stomach to dispel cold and stimulates the spleen yang; poria cocos, sweet and bland, spleen-invigorating and dampness-excreting, dried orange peel, herba elsholtziae and the like are used as ministerial drugs, so that the effects of eliminating dampness, regulating qi and removing stagnation are achieved; the chrysanthemum is used for dispelling lung-heat and wind-heat, and the kudzuvine root ascends and sends qi for clearing spleen and stomach and yang; cortex Cinnamomi has effects in invigorating primordial yang, warming spleen and stomach, removing cold accumulation, and promoting blood circulation; the liquorice is used for tonifying qi, regulating the middle warmer and harmonizing the effects of the other drugs in the recipe. The whole formula is combined, has the effects of tonifying qi and spleen, relieving muscles and skin, relieving exterior syndrome, eliminating dampness and phlegm and the like, can better regulate the internal and external damp environments of a human body, benefit the transportation and transformation functions of the spleen and the stomach, improve the immunity of the organism, and has better prevention effect on the Xinguan pneumonia. Meanwhile, the following beneficial effects are also achieved:

the quality of the Chinese medicinal materials is stable. The medicinal materials used in the invention are selected from medicinal materials with higher popularity in Hubei province and are also selected by long-term clinical application of traditional Chinese medicine, and compared with the same Chinese medicinal materials produced in other regions, the medicinal materials have better quality and curative effect and stable quality.

② the safety is high. The medicinal materials used in the invention are not only medicinal and edible medicinal materials determined in traditional Chinese medicine, but also have the characteristics of high safety, no toxic or side effect and the like through pharmacological component analysis and demonstration in modern science and technology.

And thirdly, the antivirus function is strong. In vitro antiviral research shows that the beverage has obvious inhibition and decomposition effects on SARS-CoV-2 virus, and the experiment is carried out in a P4 laboratory, so that the data is real and reliable.

Fourthly, the immunity of human body is enhanced. A large number of documents report that most of the medicinal materials in the invention have the function of obviously enhancing the body immunity of people. Meanwhile, the invention performs experiments for enhancing the immunity of organisms such as serum hemolysin determination, antibody generation cell detection, NK cell activity determination and the like, and obtains ideal effects.

Drawings

Fig. 1 is a graph of the effect of traditional Chinese medicine on serum hemolysin levels in mice (n-6);

in the figure, NC: filling with physiological saline; FGK-L, M and H: filling FGK groups with different dosages; LEM: levamisole group, P <0.001, compared to NC group;

FIG. 2 is a graph showing the effect of Chinese herbs on mouse antibody-producing cells (n-6);

fig. 3 is a graph of the effect of traditional Chinese medicine on mouse NK cell activity (n-10);

in the figure, NC: filling with physiological saline; FGK-L, M and H: filling FGK groups with different dosages; LEM: the levamisole group is injected. P <0.001, compared to NC group;

FIG. 4 is a PPI network diagram of the Chinese medicine related protein target;

FIG. 5 is a diagram of 20 pathways for analysis of Chinese medicine action target KEGG;

FIG. 6 is a 3D, 2D schematic of 3, 5-O-dicaffeoylquinic acid and SARS-CoV-23 CL hydrolase.

Detailed Description

The present invention is described in further detail below with reference to specific examples so as to be understood by those skilled in the art.

The traditional Chinese medicine for preventing the novel coronavirus pneumonia comprises, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng (artificially planted)/American ginseng, 1-14 parts of poria cocos, 2-17 parts of radix puerariae, 1-18 parts of dried ginger, 3-16 parts of dried orange peel, 2-13 parts of elsholtzia and 1-8 parts of liquorice.

The preparation process of the traditional Chinese medicine for preventing the novel coronavirus pneumonia comprises the following steps:

2) mixing coarse powders of flos Chrysanthemi, cortex Cinnamomi, pericarpium Citri Tangerinae, herba Moslae and Glycyrrhrizae radix, soaking in water for 0.5-3 hr, extracting by steam distillation for 1.5-5 hr, filtering, separating to obtain volatile oil, distillation filtrate and residue, and collecting the volatile oil, distillation filtrate and residue respectively; collecting the filtrate in another container; wherein, the weight ratio of the mixture of the five crude powder of the chrysanthemum, the cinnamon, the dried orange peel, the elsholtzia and the liquorice to the water is 1: 5-10;

3) mixing the above residues with Ginseng radix/radix Panacis Quinquefolii coarse powder, radix Puerariae coarse powder, Poria coarse powder and Zingiberis rhizoma coarse powder, adding water, heating and reflux-extracting for 2-4 hr, filtering to obtain reflux filtrate, mixing reflux filtrate with the above distilled filtrate, concentrating the mixed filtrate under reduced pressure at 50-80 deg.C, cooling to obtain fluid extract with relative density of 1.0-1.3(60 deg.C); wherein the weight ratio of the mixture of the dregs of a decoction, the ginseng/American ginseng coarse powder, the kudzu root coarse powder and the tuckahoe coarse powder to the water is 5-10;

4) adding ethanol into the fluid extract, stirring, standing, collecting supernatant, recovering ethanol, and concentrating the supernatant under reduced pressure at 50-80 deg.C to obtain traditional Chinese medicine with relative density of 1.0-1.3(80 deg.C), wherein the volume fraction of ethanol in the mixed solution of fluid extract and ethanol is 40-70%.

The experiment for preventing the novel coronavirus pneumonia by preparing the traditional Chinese medicine (hereinafter, referred to as the formula) based on the formula and the method comprises the following steps: 1. in vitro anti-SARS-CoV-2 effect

1.1 cells: Vero-E6; SARS-CoV-2 infection index: MOI 0.005

1.2 Experimental procedures:

(1) selecting 24-well plate (1ml culture medium/well), dissolving Chinese medicinal materials in purified water to obtain saturated solution (water solution), and adding 0.1 μ L and 1 μ L into each well.

(2) The virus infects Vero-E6 cell with MOI 0.005, adds some drug, collects cell supernatant 24h after infection, detects virus RNA content in supernatant by real-time RT-PCR method, calculates drug inhibition effect.

1.3 Experimental results and conclusions:

the inhibition rate of the aqueous solution on SARS-CoV-2 is 74 percent; the Chinese medicine has certain SARS-CoV-2 resisting effect.

2. Experiment for enhancing immunity of organism

2.1 materials

2.1.1 the Chinese medicine and levamisole are prepared with 0.5% CMC-Na. The experiment is provided with 3 dose groups of low, medium and high, 3.0g/kg, 6.0g/kg and 9.0g/kg, and a negative control group and a positive control group. The positive control group is levamisole (35mg/kg), the intragastric volume is 0.1ml/10g, and the negative control group is administered with physiological saline with the same volume, and the intragastric administration is carried out for 1 time every day and is continuously carried out for one month.

2.1.2 Experimental animals

ICR mice, male, SPF grade, body weight (20 ± 2) g, mouse acclimation in the animal house environment for 3d prior to testing. The experimental environmental conditions are as follows: the room temperature is 20-25 ℃, and the relative humidity is 40-70%.

2.2 test methods

2.2.1 serum hemolysin test

Each mouse was immunized by intraperitoneal injection of 0.2mL of 2% Sheep Red Blood Cells (SRBC). After 5d, removing the eyeball, taking blood, placing in a centrifugal tube for about 1h to fully separate out serum, and centrifuging to collect the serum. Set sample well and blank control well, sample well: taking 10 mu L of serum, and diluting the serum with 1mL of SA buffer solution diluted by 1: 5; adding 100 mu L of diluted serum into each hole; blank control wells: 100 μ L of 1:5 diluted SA buffer solution is added into each well, then 50 μ L of 10% (v/v) SRBC and 100 μ L of complement (diluted by SA solution or PBS solution according to a ratio of 1: 8) are sequentially added, the mixture is placed in a constant temperature water bath at 37 ℃ for heat preservation for 30min, and the mixture is centrifuged at 1500r/min for 10 min. Then 50. mu.L of each supernatant from the sample well and the blank control well was added to another 96-well plate, and 150. mu.L of Dolphin reagent was added thereto. Setting half of hemolysis hole at the same time, adding 10% (v/v) SRBC 12.5 μ L; add Duchenne reagent to 200. mu.L. Mixing well with a shaker, standing for 10min, and measuring optical density of each well at 540nm with a full-automatic enzyme labeling instrument.

2.2.2 detection of antibody-producing cells (improved method)

Each mouse was immunized by intraperitoneal injection of 0.2mL of 2% SRBC. Subjecting SRBC immunized 4-5 days after mouse cervical dislocation to sacrifice, aseptically taking spleen, gently grinding spleen with large-sized syringe inner core to obtain single cell suspension, filtering with 200 mesh screen, 1000rpm/min, 10min, removing supernatant, washing with Hank' solution for 2 times, and preparing into 1 × 10 with complete culture medium RPMI 1640⁷Individual cells/mL of spleen cell suspension. 50 μ L of 20% SRBC, 200 μ L of spleen cell suspension were added sequentially to a test tube containing 0.5mL of top layer, mixed rapidly, poured into a six-well plate and the top layer mixture was spread, and two parallel wells were made for each sample. The prepared culture plate is placed at 37 ℃ and 5% CO₂Incubate for 1h, then add 500. mu.L of complement diluted in complete medium per well (v/v, 1: 10), continue incubation for 2h, count the number of hemolytic plaques. Taking the number of parallel-hole plaquesThe average value is the number of hemolytic plaques of the sample, expressed as number of plaques/10⁷Splenocytes are shown.

2.2.3NK cell Activity assay

The spleen was aseptically removed, placed in a small dish containing an appropriate amount of sterile Hank's solution, and a piece of gauze was placed over the spleen, which was gently ground with a large syringe plunger to make a single cell suspension. Filtered through a 200-mesh screen and washed 2 times with Hank's solution, centrifuged 10min (1000r/min) each time. Discarding supernatant to bounce the cell pulp, adding 0.5mL of sterile water for 20 seconds, adding 0.5mL of 2-fold Hank's solution and 8mL of Hank's solution after lysing erythrocytes, centrifuging at 1000rpm for 10min, suspending the cells in 2mL of complete culture solution, staining with Taifenlan to count the number of viable cells, and finally adjusting the cell concentration to be 2 × 10 with RPMI 1640 complete culture solution⁷one/mL. Taking 100 mu L of target cells and effector cells respectively (the effective-target ratio is 50:1), and adding the target cells and the effector cells into a U-shaped 96-hole culture plate; target cell natural release holes are filled with 100 mu L of target cells and culture solution respectively, and the maximum release holes of the target cells are filled with 100 mu L of target cells and 100 mu L of 1% NP40 or 2.5% Triton respectively; each of the above items was plated with three multiple wells, incubated for 4 hours in a 5% CO2 incubator at 37 ℃ and then centrifuged at 1500r/min for 5min, 100. mu.l of the supernatant was aspirated from each well and placed in a flat-bottomed 96-well plate, 100. mu.l of LDH matrix solution was added simultaneously, the reaction was carried out for 3min, 30. mu.l of HCl was added to each well at 1mol/L, and the Optical Density (OD) was measured at 490nm using a microplate reader. NK cell activity requires data conversion, X ═ Sin^-1Wherein P is NK cell activity. NK cell activity was calculated as follows:

2.3 results of the experiment

2.3.1 Effect of Chinese herbs on serum hemolysin level in mice

The half hemolysis value of the mouse serum of the three dose groups is increased along with the increase of the gavage dose, and the high dose group has obvious statistical difference compared with the control group, which shows that the traditional Chinese medicine can obviously improve the mouse serum hemolysin level (figure 1).

2.3.2 Effect of Chinese herbs on mouse antibody-producing cells

As can be seen from FIG. 2, compared with the negative control group, the mice treated with the three doses of the traditional Chinese medicine can significantly increase the number of hemolytic plaques of the mice.

2.3.3 Effect of Chinese herbs on NK cell Activity in mice

The low and medium dose of the mice treated with chinese herbs significantly increased NK cell activity compared to the negative control mice (fig. 3).

And (4) conclusion: research shows that the traditional Chinese medicine has certain efficacies of resisting new coronavirus and enhancing body immunity.

3. Network pharmacology and molecular docking research for resisting neocoronary pneumonia by traditional Chinese medicine

The main active components, targets and pathways for treating the novel coronavirus pneumonia (COVID-19) are discussed based on network pharmacology, and the action mechanism of the coronavirus pneumonia is described. By means of chemical components of 9 traditional Chinese medicinal materials such as ginseng, kudzuvine root, Chinese mosla herb and the like retrieved by a traditional Chinese medicine system pharmacological analysis platform (TCMSP) database, potential active compounds are screened by taking Oral Bioavailability (OB) of more than or equal to 30% and drug-like property (DL) of more than or equal to 0.18 as thresholds. The Uniprot database is used for inquiring the target gene corresponding to the active component, and Cytoscape 3.7.1 is used for constructing a drug-compound-target network. And performing Gene Ontology (GO) function enrichment analysis and KEGG pathway enrichment analysis by using a DAVID database to predict an action mechanism of the gene ontology.

The results show that: the drug-compound-target network comprises 9 drugs, 186 compounds and 245 corresponding targets, and the protein interaction (PPI) network obtains 17 key target proteins for treating the novel coronavirus pneumonia. The GO functional enrichment analysis result shows that the GO mainly plays a role in negative regulation and positive regulation of transcription of a promoter of NA polymerase II in the biological process, and mainly relates to nucleus, plasma membrane, cytoplasm and cytoplasm in cell composition. Protein binding, enzyme binding, and the like are mainly involved in molecular functions.

KEGG pathway enrichment is primarily involved in the hepatitis b pathway, cancer pathway, pancreatic cancer pathway. Molecular docking results show that apigenin-7-O-beta-glucoside and isorhamnetin, pachymic acid G, apigenin-7-O-beta-glucoside, farnesoid-7-O-beta-D-glucoside, isorhamnetin, hesperidin, skimmin and 3, 4-O-dicaffeoylquinic acid can be potential active ingredients for resisting SARS-CoV-2. Thereby disclosing the overall regulation and control characteristics of multiple components, multiple targets and multiple ways. Wherein isorhamnetin, pachymarin G, apigenin-7-O-beta-glucoside and farnesoid-7-O-beta-D-glucoside can be potential active ingredients for resisting SARS-CoV-2, and can play a role in preventing and treating COVID-19 by acting on key targets such as SCN5A, ADRB2, CHRM3 and PGR to regulate multiple signal paths.

A computer virtual screening technology is used for searching a small molecule inhibitor for resisting a novel coronavirus (SARS-CoV-2). The method comprises the steps of searching main chemical components of medicinal materials in the Hubei tract in the Hopkichem database by taking the medicinal material name as a keyword, downloading a corresponding compound structure from the Pubchem database, determining an active region by using a protein pretreatment tool in Sybyl software by taking SARS-CoV-23 CL hydrolase protein and angiotensin converting enzyme II (ACE2) as targets, performing molecular docking on small molecules and target proteins by using a Surflex-dock module, screening out the active components by using a Total Score scoring function, and analyzing the interaction force of a protein complex combined best by using Discovery Studio software. The molecular pairing result shows that 3, 5-O-dicaffeoylquinic acid is the chemical component which is most strongly combined with SARS-CoV-23 CL hydrolase, and 3, 4-O-dicaffeoylquinic acid is the chemical component which is most strongly combined with ACE 2. The compounds have scores close to the scores of lopinavir and ritonavir serving as inhibitors and are possible potential active ingredients of chrysanthemum anti-SARS-CoV-2, and the interaction force between the compounds and proteins is mainly hydrogen bond and hydrophobic force. The research provides scientific guidance and theoretical basis for resisting SARS-CoV-2.

Example 1

The preparation process of the traditional Chinese medicine 1 for preventing the novel coronavirus pneumonia comprises the following steps:

1) the traditional Chinese medicine composition comprises, by weight, 20 parts of chrysanthemum (feverfew), 10 parts of cinnamon, 18 parts of ginseng (artificially planted), 18 parts of poria cocos, 15 parts of radix puerariae, 6 parts of dried ginger, 12 parts of dried orange peel, 10 parts of elsholtzia and 6 parts of liquorice; respectively pulverizing into coarse powder;

4) adding ethanol into the fluid extract, stirring, standing, collecting supernatant, recovering ethanol, and concentrating the supernatant under reduced pressure at 50-80 deg.C to obtain traditional Chinese medicine 1(80 deg.C) with relative density of 1.0-1.3, wherein the volume fraction of ethanol in the mixed solution of fluid extract and ethanol is 40-70%.

Example 2

The preparation process of the traditional Chinese medicine 2 in the embodiment is basically the same as that of the embodiment 1, except that:

the formula of the traditional Chinese medicine 2 comprises the following components, by weight, 25 parts of chrysanthemum (feverfew), 14 parts of cinnamon, 10 parts of ginseng (artificially planted), 9 parts of poria cocos, 7 parts of radix puerariae, 5 parts of dried ginger, 10 parts of dried orange peel, 9 parts of elsholtzia and 13 parts of liquorice.

Example 3

The preparation process of the traditional Chinese medicine 3 in this embodiment is basically the same as that in embodiment 1, except that:

the formula of the traditional Chinese medicine 3 comprises the following components, by weight, 35 parts of chrysanthemum (feverfew), 10 parts of cinnamon, 8 parts of ginseng (artificially planted), 9 parts of poria cocos, 5 parts of radix puerariae, 8 parts of dried ginger, 10 parts of dried orange peel, 7 parts of elsholtzia and 8 parts of liquorice.

Example 4

The preparation process of the traditional Chinese medicine 4 in the embodiment is basically the same as that in the embodiment 1, except that:

the formula of the traditional Chinese medicine 4 comprises the following components, by weight, 15 parts of chrysanthemum, 9 parts of cinnamon, 25 parts of ginseng (artificially planted), 10 parts of poria cocos, 6 parts of radix puerariae, 8 parts of dried ginger, 10 parts of dried orange peel, 8 parts of elsholtzia, and 9 parts of liquorice.

Example 5

The preparation process of the traditional Chinese medicine 5 in this embodiment is basically the same as that in embodiment 1, except that:

the formula of the traditional Chinese medicine 5 comprises the following components, by weight, 20 parts of chrysanthemum, 15 parts of cinnamon, 8 parts of ginseng (artificially planted), 12 parts of poria cocos, 7 parts of radix puerariae, 8 parts of dried ginger, 12 parts of dried orange peel, 9 parts of elsholtzia, and 9 parts of liquorice.

Example 6

the formula of the traditional Chinese medicine 6 comprises the following components, by weight, 10 parts of chrysanthemum, 12 parts of cinnamon, 13 parts of ginseng (artificially planted), 13 parts of poria cocos, 9 parts of radix puerariae, 10 parts of dried ginger, 14 parts of dried orange peel, 9 parts of elsholtzia, and 10 parts of liquorice.

The traditional Chinese medicines 1-6 obtained in the above examples 1-6 are used for respectively preparing traditional Chinese medicine drinks:

example 7

The traditional Chinese medicine solid beverage 1 is prepared by the following steps:

weighing 1 part of auxiliary materials obtained in example 1 according to the weight part ratio to obtain 1 part and 5 parts of traditional Chinese medicines; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein, the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetening agent is 2: 1: 2: 0.012.

adding beta-cyclodextrin into the volatile oil for inclusion to obtain an inclusion compound;

thirdly, adding starch, dextrin, a sweetening agent and the inclusion compound into the thick paste, mixing, granulating and drying to obtain a solid beverage 1; and (3) packaging, sterilizing, inspecting and warehousing the prepared solid beverage to obtain a finished product of the solid beverage 1.

Example 8

The process for preparing solid beverage 2 of this example is substantially the same as that of example 7, except that:

weighing 1 part of traditional Chinese medicine 2 and 2 parts of auxiliary materials according to the weight part ratio of the raw materials of the solid beverage 2; wherein the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; and the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 0.5: 0.5: 1: 0.02.

example 9

The process for preparing solid beverage 3 of this example is substantially the same as that of example 7, except that:

weighing 1 part of traditional Chinese medicine 3 and 26 parts of auxiliary materials according to the weight part ratio of the raw materials of the solid beverage 3; wherein the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; and the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 2: 2: 2: 0.008.

example 10

The process for preparing solid beverage 4 of this example is substantially the same as that of example 7, except that:

the solid beverage 4 is prepared by weighing 1 part of traditional Chinese medicine 4 and 9 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; and the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 3: 3: 3: 0.006.

example 11

The process for preparing solid beverage 5 of this example is substantially the same as that of example 7, except that:

the solid beverage 5 is prepared by weighing 1 part of traditional Chinese medicine 5 and 12 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; and the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 3: 3: 6: 0.015.

example 12

The process for preparing solid beverage 6 of this example is substantially the same as that of example 7, except that:

weighing 1 part of traditional Chinese medicine thick paste and 15 parts of auxiliary materials according to the weight part ratio of the raw materials of the solid beverage 6; wherein the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; and the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 3: 3: 9: 0.015.

example 13

The preparation process of the traditional Chinese medicine concentrated beverage 1 comprises the following steps:

the traditional Chinese medicine concentrated beverage 1 is prepared by the following steps:

i. weighing 1 part of traditional Chinese medicine 1 and 70 parts of auxiliary materials according to the weight part ratio; and the auxiliary materials comprise sugar alcohol, water, a sweetening agent, a preservative, a thickening agent and edible spice; the weight ratio of sugar alcohol, water, sweetener, preservative, thickener and edible flavor is 1-5: 5: 65: 0.02: 0.015: 0.8: 0.04;

adding sucrose/sugar alcohol, water, sweetener, antiseptic, thickener and edible flavor into the Chinese medicinal material 1, and mixing to obtain concentrated beverage 1; and (3) packaging, sterilizing, inspecting and warehousing the prepared solid beverage to obtain the finished product of the concentrated beverage 1.

Example 14

The process for preparing concentrated beverage 2 of this example is essentially the same as example 13, except that:

the raw materials of the concentrated beverage 2 are weighed 1 part of traditional Chinese medicine 2 and 45 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise sucrose, water, sweetener, antiseptic, thickener and edible spice; and the weight ratio of the cane sugar, the water, the sweetening agent, the preservative, the thickening agent and the edible spice is 3: 42: 0.03: 0.01: 0.5: 0.03.

example 15

The process for preparing concentrated beverage 3 of this example is essentially the same as example 13, except that:

the raw materials of the concentrated beverage 3 are weighed 1 part of traditional Chinese medicine 3 and 65 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise sucrose, water, sweetener, antiseptic, thickener and edible spice; and the weight ratio of the cane sugar, the water, the sweetening agent, the preservative, the thickening agent and the edible spice is 5: 60: 0.02: 0.015: 0.8: 0.04.

example 16

The process for preparing concentrated beverage 4 of this example is essentially the same as that of example 13, except that:

the raw materials of the concentrated beverage 4 are weighed 1 part of traditional Chinese medicine 4 and 80 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise sucrose, water, sweetener, antiseptic, thickener and edible spice; and the weight ratio of the cane sugar, the water, the sweetening agent, the preservative, the thickening agent and the edible spice is 5: 75: 0.01: 0.02: 0.4: 0.06.

example 17

The process for making concentrated beverage 5 of this example is substantially the same as example 13, except that:

the concentrated beverage 5 is prepared by weighing 1 part of traditional Chinese medicine 5 and 15 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise sugar alcohol, water, sweetener, antiseptic, thickener and edible essence; and the weight ratio of sugar alcohol, water, sweetener, preservative, thickener and edible flavor is 5: 10: 0.01: 0.004: 0.2: 0.01.

example 18

The process for preparing concentrated beverage 6 of this example is essentially the same as that of example 13, except that:

the concentrated beverage 6 is prepared by weighing 1 part of traditional Chinese medicine 6 and 30 parts of auxiliary materials according to the weight part ratio; wherein the adjuvants comprise sucrose, water, sweetener, antiseptic, thickener and edible spice; and the weight ratio of the cane sugar, the water, the sweetening agent, the preservative, the thickening agent and the edible spice is 5: 25: 0.02: 0.008: 0.3: 0.01.

TABLE 1 taste evaluation results

Group of	Taste evaluation results	Scoring
			Solid beverage 1	Smooth in mouth, sweet and mellow, strong aftertaste, no bitter and astringent, and fragrant in smell	5
Solid beverage 2	Smooth and sweet, mellow, strong aftertaste and no smellBitter and astringent taste and fragrant smell	4
			Solid beverage 3	Smooth in mouth, fragrant and sweet, strong aftertaste, no bitter and astringent, and light and fragrant smell	3
Solid beverage 4	Smooth in mouth, sweet and mellow, general aftertaste, no bitter and astringent, and light and fragrant in smell	3
			Solid beverage 5	Smooth in mouth, fragrant and sweet, strong aftertaste, no bitter and astringent, and fragrant in smell	2
Solid beverage 6	Smooth in mouth, sweet and mellow, general in aftertaste, too sweet and fragrant in smell	1
			Concentrated beverage 1	Smooth in mouth, sweet and mellow, strong aftertaste, no bitter and astringent, and fragrant in smell	5
Concentrated beverage 2	Smooth in mouth, sweet and mellow, strong aftertaste, no bitter and astringent, and fragrant in smell	4
			Concentrated beverage 3	Smooth in mouth, fragrant and sweet, strong aftertaste, no bitter and astringent, and light and fragrant smell	3
Concentrated beverage 4	Smooth in mouth, sweet and mellow, general aftertaste, no bitter and astringent, and light and fragrant in smell	3
			Concentrated beverage 5	Smooth in mouth, fragrant and sweet, strong aftertaste, no bitter and astringent, and fragrant in smell	2
Concentrated beverage 6	Smooth in mouth, sweet and mellow, general in aftertaste, too sweet and fragrant in smell	1

Grading standard: the optimum is-5 minutes, the optimum is-4 minutes, the better is-3 minutes, the average is-2 minutes, and the worse is-1 minute.

And (4) evaluating the mouthfeel: as can be seen from table 1 above, both the solid beverage 1 and the concentrated beverage 1 had the best mouthfeel.

Other parts not described in detail are prior art. Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.

Claims

1. A traditional Chinese medicine for preventing novel coronavirus pneumonia is characterized in that: the traditional Chinese medicine comprises, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng/American ginseng, 1-14 parts of poria cocos, 2-17 parts of kudzu vine root, 1-18 parts of dried ginger, 3-16 parts of dried orange peel, 2-13 parts of elsholtzia and 1-8 parts of liquorice.

2. The traditional Chinese medicine for preventing the novel coronavirus pneumonia according to claim 1, which is characterized in that: the traditional Chinese medicine comprises, by weight, 15-30 parts of chrysanthemum, 10-20 parts of cinnamon, 8-18 parts of ginseng/American ginseng, 5-10 parts of poria cocos, 5-15 parts of kudzu vine root, 3-15 parts of dried ginger, 5-12 parts of dried orange peel, 5-10 parts of elsholtzia and 3-6 parts of liquorice.

3. The traditional Chinese medicine for preventing the novel coronavirus pneumonia according to claim 1, which is characterized in that: the traditional Chinese medicine comprises the raw material components of, by weight, 20 parts of chrysanthemum, 10 parts of cinnamon, 18 parts of ginseng/American ginseng, 18 parts of poria cocos, 15 parts of radix puerariae, 6 parts of dried ginger, 12 parts of dried orange peel, 10 parts of elsholtzia and 6 parts of liquorice.

4. The preparation process of the traditional Chinese medicine for preventing the novel coronavirus pneumonia in claim 1 is characterized in that: the method comprises the following steps:

1) comprises chrysanthemum, cinnamon, ginseng/American ginseng, tuckahoe, kudzu root, dried ginger, dried orange peel, Chinese mosla herb and liquorice in parts by weight; respectively pulverizing into coarse powder;

5. The preparation process of the traditional Chinese medicine for preventing the novel coronavirus pneumonia according to claim 4, which is characterized in that: in the step 2), the weight ratio of the mixture of the coarse powder of five medicinal materials including chrysanthemum, cinnamon, dried orange peel, elsholtzia and liquorice to water is 1: 5-10; soaking for 0.5-3h, and extracting for 1.5-5h by steam distillation;

in the step 3), the weight ratio of the mixture of the medicine residues, the ginseng/American ginseng coarse powder, the kudzuvine root coarse powder and the tuckahoe coarse powder to water is 5-10, the extraction time is 2-4h, and the relative density of the clear paste is 1.0-1.3.

6. The preparation process of the traditional Chinese medicine for preventing the novel coronavirus pneumonia according to claim 4, which is characterized in that: in the step 4), the volume fraction of ethanol in the mixed solution of the clear paste and the ethanol is 40-70%, the standing time is 12-24 hours, and the relative density of the thick paste is 1.0-1.3.

7. A traditional Chinese medicine drink is characterized in that: the traditional Chinese medicine beverage comprises, by weight, 1 part of the traditional Chinese medicine according to claim 1 and 2-80 parts of auxiliary materials.

8. The herbal beverage of claim 7, wherein:

the traditional Chinese medicine beverage is a solid beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 2-30 parts of auxiliary materials in parts by weight; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetener is 1: 1-3: 1-3: 4-20: 0.003-0.02;

9. The herbal beverage of claim 8, wherein:

the traditional Chinese medicine beverage is a solid beverage, and the raw materials of the traditional Chinese medicine beverage comprise 1 part of traditional Chinese medicine and 5 parts of auxiliary materials in parts by weight; and the adjuvants comprise starch, dextrin, BETA-cyclodextrin and sweetener; wherein, the weight ratio of the starch, the dextrin, the beta-cyclodextrin and the sweetening agent is 2: 1: 2: 0.012;

10. a preparation process of a traditional Chinese medicine beverage is characterized by comprising the following steps: the method comprises the following steps:

1) the traditional Chinese medicine is prepared by the following steps:

a. the traditional Chinese medicine composition comprises, by weight, 10-36 parts of chrysanthemum, 8-25 parts of cinnamon, 6-23 parts of ginseng/American ginseng, 1-14 parts of poria cocos, 2-17 parts of radix puerariae, 1-18 parts of dried ginger, 3-16 parts of dried orange peel, 2-13 parts of elsholtzia and 1-8 parts of liquorice; respectively pulverizing into coarse powder;

if the traditional Chinese medicine beverage is a solid beverage,

or, if the traditional Chinese medicine beverage is a concentrated beverage,