CN103735662A - Pharmaceutical composition of ginseng and donkey-hide gelatin as well as preparation method and use thereof - Google Patents

Pharmaceutical composition of ginseng and donkey-hide gelatin as well as preparation method and use thereof Download PDF

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CN103735662A
CN103735662A CN201310751732.XA CN201310751732A CN103735662A CN 103735662 A CN103735662 A CN 103735662A CN 201310751732 A CN201310751732 A CN 201310751732A CN 103735662 A CN103735662 A CN 103735662A
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pharmaceutical composition
extract
preparation
parts
quality
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秦玉峰
周祥山
张淹
李民
王春艳
李士栋
张路
辛杰
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NATIONAL DONKEY-HIDE GELATIN TRADITIONAL CHINESE MEDICINE ENGINEERING TECHNOLOGY RESEARCH CENTER
Shandong Dong E E Jiao Co Ltd
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NATIONAL DONKEY-HIDE GELATIN TRADITIONAL CHINESE MEDICINE ENGINEERING TECHNOLOGY RESEARCH CENTER
Shandong Dong E E Jiao Co Ltd
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Priority to CN201310751732.XA priority Critical patent/CN103735662A/en
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Abstract

The invention relates to a pharmaceutical composition of ginseng and donkey-hide gelatin with effects of enhancing immunity and resisting fatigue, and a preparation method of the pharmaceutical composition. The pharmaceutical composition is prepared from the following crude drugs in parts by weight: 2-6 parts of ginseng, 2-6 parts of poria cocos, 1-3 parts of donkey-hide gelatin, 1-3 parts of cordyceps militaris and 1-3 parts of hawthorn. The pharmaceutical composition disclosed by the invention is a pure traditional Chinese medicine preparation, and the drug components have the action, and also have synergistic effects, so as to achieve the targets of improving the immunity, resisting fatigue and relieving sub-health in common. Meanwhile, a plurality of screening tests can be carried out on the preparation technology. The optimal extracting technology and drying method are determined by test and comparison, and the effective ingredients of each drug component can be stored to the maximal extent, so as to improve the drug effect. In design of a dosage form, granules which are convenient to take and good in taste are adopted, and the pharmaceutical composition is simple in preparation technology, fewer in additive and healthier.

Description

A kind of ginseng donkey-hide gelatin pharmaceutical composition and its production and use
Technical field
The present invention relates to a kind of pharmaceutical composition, particularly a kind of composition and method of making the same and purposes that contains Radix Ginseng and Colla Corii Asini medicine.
Background technology
Hypoimmunity, fatiguability are a kind of sub-health states.Whole nation mental health instructs and educates the investigation report demonstration of issuing in work of popular science seminar, and China's sub-health population accounts for 70%, and main manifestations is the symptoms such as hypoimmunity, fatiguability, insomnia.Fitness guru proposes subhealth state can affect work, the learning efficiency, reduces quality of life; Sub-health state, through worsening, also can be converted into disease, only has through treatment and just can improve, for people bring body & mind puzzlement; And the hotbed of hiding of subhealth state or some major diseases, as cardiovascular and cerebrovascular disease, early-stage cancer etc., if can not find in time, will bring irremediable consequence.In addition, a lot of people's subhealth state shows mentally, as detest society, the exposed population group that is unwilling, to people distrust, suspicious, forgetful etc., all can people at one's side be affected.Therefore, improve sub-health state very urgent.
Object based on improving body immunity and resisting fatigue and then alleviation sub-health state, we have researched and developed a kind of ginseng donkey-hide gelatin pharmaceutical composition.Pharmaceutical composition of the present invention can obviously improve body immunity, resisting fatigue, improves sub-health state, improves people's the mental status, and this will promote the raising of people's work efficiency and quality of life greatly.Drug regimen raw material of the present invention all adopts integration of edible and medicinal herbs medical material, can long-term taking, and make the dosage form being convenient for carrying, be suitable for taking whenever and wherever possible and for a long time.
Summary of the invention
The object of the invention is to disclose a kind of ginseng donkey-hide gelatin pharmaceutical composition.Described pharmaceutical composition has the effect of enhancing immunity, resisting fatigue.
Another object of the present invention is also to disclose the preparation method of described pharmaceutical composition.
In addition, the present invention also aims to disclose the purposes of described pharmaceutical composition.
Pharmaceutical composition of the present invention is to be made by the crude drug of following weight proportioning: 2~6 parts of Radix Ginsengs, 2~6 parts, Poria, 1~3 part, Colla Corii Asini, 1~3 part of Cordyceps militaris (L.) Link., 1~3 part of Fructus Crataegi.
Preferably, the weight proportion of described pharmaceutical composition is: 3 parts of Radix Ginsengs, 3 parts, Poria, 1 part, Colla Corii Asini, 1 part of Cordyceps militaris (L.) Link., 1 part of Fructus Crataegi.
Radix Ginseng, Colla Corii Asini, Cordyceps are conventional health-care tonic medicines, all have the effect of resisting fatigue, enhancing immunity, but they have again himself feature and effect.
Wherein, Radix Ginseng " spasmolytic is throbbed with fear for main tonifying five ZANG-organs, peace spirit, except pathogen, improving eyesight, and happy Fructus Alpiniae Oxyphyllae ".Research discovery, Radix Ginseng has obvious antifatigue effect, antitumor, raising immunity of organism to human body, can improve depression, alleviates anxiety, has effect anticancer and anti-cancer.In pharmaceutical composition of the present invention, Radix Ginseng is by invigorating primordial QI, effectively alleviating physical fatigue.
Colla Corii Asini is tonifying blood and arresting bleeding, moist dry good medicine, at blood deficiency and yellow complexion, dizzy cardiopalmus, unable, the vexed insomnia of flesh flaccidity, stirring-up of pathogenic wind in the interior resulting from deficiency, xeropulmonary cough, the phthisical cough aspects such as metrorrhagia of spitting blood, spit blood, have blood in stool, there is significant curative effect, Colla Corii Asini also has hematosis, and can improve calcium balance the conversion of promotion health human lymphocyte in body.In pharmaceutical composition of the present invention, Colla Corii Asini nourishing blood YIN nourishing, by enriching blood and improving calcium ion balance, improves user's immunity.
Cordyceps is that people praise highly doubly a kind of medicine extremely in recent years, its effect is mainly to regulate immune system, and it can increase immune system cell, organize quantity, and enhancing antibody produces, increase and to engulf, killer cell quantity, strengthen its function, can turn down again the function of some immunocyte.In addition, Cordyceps can also regulate heart, liver, respiratory system and renal function, experiment discovery, and Cordyceps can be improved cellular energy, improves body tolerance to cold and also lessens fatigue.In pharmaceutical composition of the present invention, adopt the Cordyceps of given dose to regulate each internal organs of health and immune situation, improve user's immunity.In the present invention, consider Cordyceps Resources present situation in short supply, adopted generally acknowledged succedaneum " Cordyceps militaris (L.) Link. ", in Cordyceps militaris (L.) Link., the content of adenosine will be higher than Cordyceps, can kidney-reinforcing Yang-strengthening, be especially applicable to work strain, male that pressure is large.
In addition, in pharmaceutical composition of the present invention, also add Poria and Fructus Crataegi, can spleen benefiting and stimulating the appetite digestion promoting, make to mend and impartially, taste and oiliness.Poria slightly sweet flavor is flat, enters the heart, lung, spleen channel, has eliminating dampness and diuresis, invigorating the spleen and regulating the stomach, the effect of mind tranquilizing and the heart calming.Ancients claim that Poria is " god medicine " at 4 o'clock, and its effect is very extensive, by it and various compatibility of drugs, no matter cold, temperature, wind, all diseases that wets can be brought into play its unique effects.Modern medicine also confirms that it can enhancing human body immunity function.In pharmaceutical composition of the present invention, by the fortune humidifying water effect of its uniqueness of Poria, adjust user's health water metabolism and dry, alleviate health sense of heaviness, thus effective fatigue alleviating.Fructus Crataegi sour in the mouth, sweet, slightly warm in nature, in pharmaceutical composition of the present invention, the main effect of Fructus Crataegi is promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis.
Pharmaceutical composition of the present invention can adopt the conventional the whole bag of tricks preparation in this area, can add the conventional adjuvant in this area to make the dosage forms such as tablet, capsule, oral liquid, granule.Described adjuvant can comprise solvent, disintegrating agent, correctives, antiseptic, coloring agent, binding agent, lubricant, substrate etc.The method adopting has liquid phase extraction method (conventional water extraction and alcohol extracting method), the combination of solid-state mixing method or above-mentioned two kinds of methods.
The dose of pharmaceutical composition of the present invention is crude drug quality 3~36g/ time, preferably 9~27g/ time, and 1~3 times/day; Preferably 1 times/day.
Pharmaceutical composition of the present invention can be made into the granule that facilitates portable.
The present invention also provides a kind of preparation method of granule of described pharmaceutical composition.
The preparation method of medicament composition granule agent of the present invention is as follows:
1. extract: Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by consumption, add water, extract merging filtrate 2~5 times;
2. change glue: Colla Corii Asini is placed in to suitable container, adds the water of 1~3 times of amount of Colla Corii Asini quality, heating is also constantly stirred to Colla Corii Asini and dissolves completely;
3. concentrated: it is 1.1~1.35 that herbal extract is concentrated into relative density with glue merging;
4. dry: medicinal liquid to be dried, to obtain dried particles.
Wherein, step 1. in, described extraction preferably 3 times;
Described extraction can be carried out as follows: add 3~6 times of water gagings of quality of medicinal material, soak 0.1~2 hour, extract 30~120 minutes, filter; Add for the second time 3~6 times of water gagings of quality of medicinal material, extract 30~90 minutes, filter; Add for the third time 2~4 times of water gagings of quality of medicinal material, extract 30~60 minutes, filter, merge three times filtrate;
Described extraction can be taked dynamic low temperature extraction technology, as liquid-phase reflux extracts.Can under the temperature range of 65~95 ℃ and 1~3 atmospheric pressure limit, carry out liquid-phase reflux extraction for instance, be to reduce production equipment cost, preferably under higher temperature (88~95 ℃) and lower air pressure range (normal pressure), carries out.
Preferably, described extraction is carried out as follows: Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by above-mentioned consumption, add 6 times of water gagings, soak 1 hour, extract 90 minutes, filter; Add for the second time 6 times of water gagings, extract 60 minutes, filter; Add for the third time 4 times of water gagings, extract 40 minutes, filter, merge three times filtrate;
Step 2. in, preferably 2 times of the water yields;
Step 3. in, described density is preferably 1.25;
Step 4. in, described dry employing vacuum belt type drying method.
Vacuum belt type drying is the contact vacuum drying method of a kind of continuous feed, continuous discharge form, and the dry mobility of particle making is good, can direct compression or encapsulating capsule; The outward appearance of granule is good simultaneously, also has the open structure of microcosmic, and instant capacity is fabulous, is particularly suitable for preparing instant (electuary) type product, greatly the class of improving product.
Concerning pharmaceutical composition of the present invention, described dry employing vacuum belt drier is realized, and band-type vacuum drier is respectively at the continuous feed of the two ends of fuselage, continuous discharge, under vacuum state, complete dry, pulverize, granulate; Product is in whole dry run, and in vacuum, enclosed environment, dry run is gentle, for natural extract Tetramune, can keep to greatest extent its color, obtains high-quality final products.General baking temperature can be adjusted 25 ℃~150 ℃ according to material technological requirement, preferably 60~90 ℃, heat sensitive material invariance, not microbiological contamination (product temperature generally can remain on 40~60 ℃) like this, can also guarantee that high viscosity, difficult dry colloidal liquid are dried.
In addition, in step granule 4., can add pharmaceutically acceptable carrier and/or excipient, adopt traditional preparation technology to can be made into clinical acceptable any dosage form, as tablet, pill, capsule, granule, electuary etc.
Preferably, the preparation method of described medicament composition granule agent is as follows:
1. extract: Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by above-mentioned consumption, add 6 times of water gagings of quality of medicinal material, soak 1 hour, extract 90 minutes, filter; Add for the second time 6 times of water gagings, extract 60 minutes, filter; Add for the third time 4 times of water gagings, extract 40 minutes, filter, merge three times filtrate;
2. change glue: Colla Corii Asini adds 2 times of water gagings, heating is constantly stirred to Colla Corii Asini and dissolves completely;
3. concentrated: it is 1.25 that herbal extract is concentrated into relative density with glue merging;
4. dry: to adopt vacuum belt type drying method to be dried medicinal liquid, obtain dried particles.
The magical effect of Radix Ginseng derives from it and is rich in ginsenoside, ginseng polysaccharide, and aminoacid, polypeptide, flavone, volatile oil and various trace elements, wherein in Radix Ginseng, exclusive nearly more than 50 ginsenosides that plant are the key substance that human body is worked by medical evidence.The present invention is directed to the physicochemical property of the main pharmacodynamics composition of different pharmaceutical component in prescription, selected comparatively gentle low-temperature extraction isolation technics, some thermal sensitivity active ingredient are transferred to greatest extent and extracted in solvent, avoided high temperature in the conventional leaching process destruction to thermal sensitivity composition simultaneously.
In addition, the present invention adopts vacuum and low temperature concentrate drying technology to be dried glue class Chinese traditional compound medicine, having solved Yin Wendu in glue class Chinese medicine dry run, compared with technical barriers such as gelatinizing high and that be prone to or coking, also makes some other thermal sensitivity active ingredient in preparation process, well shift and retain simultaneously.
The dose of medicament composition granule agent of the present invention is 1~9g/ time, preferably 3~6g/ time; 1~3 times/day; Preferably 1 times/day.
Pharmaceutical composition of the present invention is on the basis of numerous Chinese herbal medicine, to carry out compatible combination to form, and is pure Chinese medicinal preparation, existing himself the effect of above-mentioned various medicine components, synergism influences each other again, jointly reach raising immunity, resisting fatigue, the object of alleviation subhealth state.Pharmaceutical composition of the present invention has the function that improves immunity, resisting fatigue, through pharmacological experiment study, shows that pharmaceutical composition of the present invention can, by improving the level of mice serum hemolysin, promote bone-marrow-derived lymphocyte propagation, thereby significantly improve immunity of organisms; Also significantly increase in addition the mice burden swimming time, show that pharmaceutical composition of the present invention has obvious antifatigue effect.The present invention has simultaneously carried out a large amount of screening tests on preparation process, through overtesting comparison, has determined optimal extract process and drying means, can preserve to greatest extent the effective ingredient of each drug component, thereby improves drug effect.In dosage form design, also adopt the granule of taking convenience, features good taste, preparation technology is simple, and additive is few, more healthy.
The specific embodiment
Following examples are for illustrating but not limiting technical scheme of the present invention, and if no special instructions, the method adopting is the conventional method in this area; Vacuum belt continuous drier (BVD-6120, Wenzhou imperial examinations light industry and machinery company limited).
Embodiment 1
1. Extraction technique determines
For reaching good extraction effect, guarantee that composition extracts completely, using the content of total saponins in concentrated solution as investigating index, design extraction process.
The investigation of 1.1 extraction times
Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by the consumption of table 1, add water, all soak for the first time 1 hour, extract 2~5 times, merging filtrate, being concentrated into relative density is 1.20, measures the content of total saponins in concentrated solution;
Table 1 extraction time experimental result
Result is as shown in table 1, obviously, extracts 3 times, and the effective ingredient of 4 times and 5 times is more, but there is no notable difference, for the purpose of saving, and extraction time preferably 3 times.
The investigation of 1.2 extraction times and solvent load
Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by the consumption of table 2, add water, all soak for the first time 1 hour, extract 3 times, merging filtrate, being concentrated into relative density is 1.20, measures the content of total saponins in concentrated solution.
Table 2 extraction time experimental result
Result is as shown in table 2, obviously, by second group of extracting mode, is undertaken, and the effective ingredient in concentrated solution is more, therefore, the present invention selects following process conditions: take Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi crude drug, add 6 times of water gagings of quality of medicinal material, soak 1 hour, extract 90 minutes, filter; Add for the second time 6 times of water gagings of quality of medicinal material, extract 60 minutes, filter; Add for the third time 4 times of water gagings of quality of medicinal material, extract 40 minutes, filter, merge three times filtrate.
Embodiment 2
Press following weight proportion weighting raw materials: 2 parts of Radix Ginsengs, 2 parts, Poria, 1 part, Colla Corii Asini, 1 part of Cordyceps militaris (L.) Link., 2 parts of Fructus Crataegis.
The extracting method of selecting by embodiment 1 extracts, and then gets Colla Corii Asini, adds 3 times of water gagings of Colla Corii Asini quality, and heating is constantly stirred to Colla Corii Asini and dissolves completely; Herbal extract and glue are merged to be concentrated into relative density be 1.25, add the starch of 1 part of raw-material weight umber, adopt vacuum belt type drying method to be dried medicinal liquid, obtain dried particles.
Embodiment 3
Press following weight proportion weighting raw materials: 3 parts of Radix Ginsengs, 3 parts, Poria, 1 part, Colla Corii Asini, 1 part of Cordyceps militaris (L.) Link., 1 part of Fructus Crataegi.
The extracting method of selecting by embodiment 1 extracts, and then gets Colla Corii Asini, adds 2 times of water gagings of Colla Corii Asini quality, and heating is constantly stirred to Colla Corii Asini and dissolves completely; Herbal extract and glue are merged to be concentrated into relative density be 1.25, adopt vacuum belt type drying method to be dried medicinal liquid, obtain dried particles.
Embodiment 4
Press following weight proportion weighting raw materials: 3 parts of Radix Ginsengs, 6 parts, Poria, 2 parts, Colla Corii Asini, 1 part of Cordyceps militaris (L.) Link., 3 parts of Fructus Crataegis.
The extracting method of selecting by embodiment 1 extracts, and then gets Colla Corii Asini, adds 1 times of water gaging of Colla Corii Asini quality, and heating is constantly stirred to Colla Corii Asini and dissolves completely; Herbal extract and glue are merged to be concentrated into relative density be 1.25, adopt vacuum belt type drying method to be dried medicinal liquid, obtain dried particles, add the magnesium stearate of 1 part of raw-material weight umber, tabletting.
Embodiment 5
1. material
1.1 reagent RPMI1640 culture medium (being provided by GIBCO company); Lipopolysaccharide (being provided by Sigma company).
1.2 instrument Sartorius-BS323S electronic balances, Sai Duolisi scientific instrument company limited; Sigma3K30 low-temperature and high-speed centrifuge, German Sigma company; 722 type grating spectrophotometers, Shanghai San analytical tool factory; LDZX-75KB vertical pressure steam sterilizer, Shenan Medical Appliances Factory, Shanghai.
2. animal Kunming mouse, SPF level, 18~22g, male and female half and half.
3 experiment contents
3.1 impacts of pharmaceutical composition of the present invention on mice serum hemolysin
50 of mices, weight 18~22g, male and female half and half.Be divided into normal saline matched group, positive drug control group (4g/kg), pharmaceutical composition of the present invention (getting the granule of embodiment 3 preparations) low (0.2g/kg), in totally 5 groups of (0.4g/kg), high (0.8g/kg) dosage groups, 10 every group.Adaptability is raised after one day and is started administration.Administration the 3rd day, the SRBC sensitization of every mouse peritoneal injection 30%, 4d after sensitization, plucks eyeball after last administration 1h and gets blood, only puts 1h, the centrifugal 10min of 3000r/min, separation of serum, for measuring.
Hemolysin quantitative assay: get dilution 100 times after serum 1.0ml add 30%SRBC0.5ml, add 10% complement 1.0ml and enter reaction tube, put after 37 ℃ of water bath heat preservation 30min, move to cessation reaction in ice-water bath, the centrifugal 10min of 2000r/min, get supernatant 1ml and place 10min, in 540nm place, read absorbance.
3.2 impacts that pharmaceutical composition is bred bone-marrow-derived lymphocyte
Aseptic condition is got 2 mouse spleens, with D-HANK ' S liquid, fully washs, and in having the plate of cold preservation buffer, rejects fat and connective tissue.Spleen is shredded, with asepsis injector nook closing member, pulverize gently spleen tissue, make individual cells enter buffer through nylon cloth, solution is placed in to centrifuge tube, after the centrifugal 5min of 1500r/min, abandon supernatant.PBS buffer washed cell 2 times, is finally transferred in the culture dish that 10%NBS-RPMI-1640 culture fluid is housed, and with 10%NBS-RPMI-1640 culture fluid, prepares 4 * 10 6the mouse boosting cell suspension of individual/ml.
In 96 orifice plates, every hole adds 90 μ l splenocyte suspensions, then adds 10 μ l lipopolysaccharide solution, and making every hole lipopolysaccharide volume parameter is 40 μ l/ml.1640 culture medium are prepared this medicinal composition solution of 1 μ g/ml, 10 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml, 500 μ g/ml, 1mg/ml, 5mg/ml, 10mg/ml, 50mg/mL, after cell attachment, the reagent of above-mentioned mass concentration is got respectively to 10 μ l and add spleen cell cultures plate, each mass concentration is established parallel 6 holes, separately establishes culture medium control wells and splenocyte suspension control wells.The final mass concentration of dosing holes is respectively 0.1 μ g/ml, 1 μ g/mL, 5 μ g/ml, 10 μ g/ml, 20 μ g/ml, 50 μ g/ml, 100 μ g/ml, 500 μ g/ml, 1mg/ml, 2mg/mL, 5mg/ml.96 well culture plates are put to 5%CO 2, 37 ℃ of CO 2in incubator, cultivate 48h, mtt assay is measured 492nm place light absorption value.
4. results and analysis
4.1 impacts of pharmaceutical composition of the present invention on mice serum hemolysin
Experimental result demonstration, with the comparison of blank group, each administration group serum hemolysin has obvious rising, and difference has statistical significance (P < 0.05); Pharmaceutical composition low dosage administration group serum hemolysin of the present invention is lower than positive controls, and result has statistical significance (P < 0.05), in Table 3.
The impact of 4.2 pharmaceutical compositions of the present invention on mice bone-marrow-derived lymphocyte propagation
Experimental result shows, during drug quality concentration 0.005~1mg/ml, pastille is cultivated basis set OD value contrast blank (drug quality concentration is 0mg/ml), OD value raises, result has statistical significance (P < 0.05), and drug quality concentration can promote mice bone-marrow-derived lymphocyte in-vitro multiplication between the medicinal composition solution of the present invention of 0.005~1mg/ml.In Table 4.
Table 3 pharmaceutical composition of the present invention on mice serum hemolysin affect result (X ± S, n=10)
Group Dosage/(g/kg) Weight/g before test Weight/g after test OD value
Blank group -- 18.5±0.96 27.9±4.02 0.2299±0.0196
Positive controls 4.0 19.1±0.92 26.3±2.35 0.3365±0.9854 #
Low dose group 0.2 18.8±0.75 27.5±2.21 0.2729±0.0531#△
Middle dosage group 0.4 18.7±0.95 26.7±3.01 0.3225±0.0763 #
High dose group 0.8 18.6±1.03 27.4±1.92 0.3225±0.7621 #
Note: with blank comparison, #p < 0.05, ##p < 0.01; With positive control comparison, Δp < 0.05, Δ Δp < 0.01.
Table 4 pharmaceutical composition of the present invention affects result (X ± S, n=6) to mice bone-marrow-derived lymphocyte propagation
The whole mass concentration of medicine/(mg/ml) OD value
0 0.016
0.0001 0.022±0.008
0.0010 0.017±0.004
0.0050 0.145±0.087#
0.0100 0.226±0.118##
0.0200 0.205±1.101##
0.0500 0.184±0.092##
0.1000 0.147±±0.075##
0.5000 0.024±0.005#
1.0000 0.057±0.027#
2.0000 0.039±0.021
5.0000 0.025±0.023
Note: with the comparison of simple cell control wells, #P < 0.05, ##P < 0.01.
This experimental result shows that pharmaceutical composition of the present invention can strengthen the ability that mice hemolysin forms, and points out it can improve antigen induction circulating antibody level, thereby realizes the effect that improves humoral immunity of organism power.By observing the impact of pharmaceutical composition of the present invention on bone-marrow-derived lymphocyte propagation, result shows that the pharmaceutical composition of the present invention of certain mass concentration range can improve through lipopolysaccharide-induced mice bone-marrow-derived lymphocyte conversion ratio.These two results of study can show, pharmaceutical composition of the present invention can have certain facilitation to mouse humoral immune, cellular immune function.
Embodiment 6
1. material
1.1 animals: Kunming mouse, 18~22g, male and female half and half, totally 30.
1.2 equipment: water vat, heavy burden thing (each 2g), stopwatch, thermometer.
1.3 medicines: get the present composition (getting the granule of embodiment 3 preparations) and make respectively in right amount 20mg/ml(low dosage), solution 40mg/ml(high dose).
2. experimental technique and step
30 of Kunming mouses getting body weight 18~22g, male and female half and half, are divided into 3 groups immediately, weigh respectively, numbering.2 groups and 3 groups of administration group gavages every day give low dosage and the high dose solution (20ml/kg) of this composite medicine (getting the granule of embodiment 3 preparations), be equivalent to respectively 1 and 2 times of clinical human body consumption, every of control group mice gives equal-volume normal saline once a day, continuous 5 days.Administration in the 6th day by the heavy burden thing of mouse tail bundle 2g, was put into 15 ℃ of water vats after 30 minutes, and with manual time-keeping, when observing mouse head, submerged in water and can not the person of emerging to be muscle power 10 seconds and to exhaust, timing at once ends up being the swimming time of mice.Statistics is respectively organized the swimming time of mice, calculates the average swimming time of respectively organizing mice.Relatively carry out t check with matched group, detect its significance.
3. experimental result
The swimming time experimental result of table 5 mice
Group Number of animals Dosage Route of administration The average swimming time of mice (min)
Matched group 10 20ml/kg po 2.52±0.72
Low dose group 10 20ml/kg po 3.85±0.63*
High dose group 10 20ml/kg po 4.25±0.76**
Note: * P < 0.05, * * P < 0.01.
By above result of the test, show the swimming time that pharmaceutical composition of the present invention can significant prolongation mice, illustrate that it has obvious antifatigue effect.

Claims (10)

1. a ginseng donkey-hide gelatin pharmaceutical composition, is characterized in that, described pharmaceutical composition is to be made by the crude drug of following weight proportioning: 2~6 parts of Radix Ginsengs, 2~6 parts, Poria, 1~3 part, Colla Corii Asini, 1~3 part of Cordyceps militaris (L.) Link., 1~3 part of Fructus Crataegi.
2. pharmaceutical composition as claimed in claim 1, is characterized in that, the weight proportion of described pharmaceutical composition is: 3 parts of Radix Ginsengs, 3 parts, Poria, 1 part, Colla Corii Asini, 1 part of Cordyceps militaris (L.) Link., 1 part of Fructus Crataegi.
3. the preparation method of pharmaceutical composition as claimed in claim 1 or 2, comprises the following steps successively:
1. extract: Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by consumption, extract merging filtrate 2~5 times;
2. change glue: Colla Corii Asini is placed in to container, and adding quality is the water of 1~3 times of amount of Colla Corii Asini quality, heating is also constantly stirred to Colla Corii Asini and dissolves completely;
3. concentrated: it is 1.1~1.35 that herbal extract is concentrated into relative density with glue merging;
4. dry: medicinal liquid to be dried, to obtain dried particles.
4. preparation method as claimed in claim 3, is characterized in that, step 1. in, described in be extracted as 3 times.
5. preparation method as claimed in claim 4, is characterized in that, described extraction is carried out as follows: add 3~6 times of water gagings of quality of medicinal material, soak 0.1~2 hour, extract 30~120 minutes, filter; Add for the second time 3~6 times of water gagings of quality of medicinal material, extract 30~90 minutes, filter; Add for the third time 2~4 times of water gagings of quality of medicinal material, extract 30~60 minutes, filter, merge three times filtrate.
6. the preparation method as described in claim 4 or 5, is characterized in that, described extraction is carried out as follows: Radix Ginseng, Poria, Cordyceps militaris (L.) Link., Fructus Crataegi are taken to crude drug by above-mentioned consumption, add 6 times of water gagings of quality of medicinal material, soak 1 hour, extract 90 minutes, filter; Add for the second time 6 times of water gagings of quality of medicinal material, extract 60 minutes, filter; Add for the third time 4 times of water gagings of quality of medicinal material, extract 40 minutes, filter, merge three times filtrate.
7. preparation method as claimed in claim 3, is characterized in that, step 2. in, the water yield is 2 times; Step 3. in, described density is 1.25.
8. the preparation method as described in claim 3~6 any one, is characterized in that, described in be extracted as dynamic low temperature extraction.
9. preparation method as claimed in claim 3, is characterized in that, step 4. in, described dry employing vacuum belt type drying.
10. the purposes of pharmaceutical composition as claimed in claim 1 or 2, is characterized in that, for enhancing immunity or resisting fatigue.
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CN106266515A (en) * 2016-08-26 2017-01-04 湖南湘雅制药有限公司 Ginseng Siberian cocklebur grass compositions, ginseng Siberian cocklebur grass beverage and preparation method
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CN104872680A (en) * 2015-06-25 2015-09-02 绿之韵生物工程集团有限公司 Healthcare food composition capable of boosting immunity
CN106266515A (en) * 2016-08-26 2017-01-04 湖南湘雅制药有限公司 Ginseng Siberian cocklebur grass compositions, ginseng Siberian cocklebur grass beverage and preparation method
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CN109294970A (en) * 2017-07-24 2019-02-01 东阿阿胶股份有限公司 Application of the donkey-hide gelatin in the cell culture for preparing rush cell division breeding, anti-apoptotic
CN109294970B (en) * 2017-07-24 2021-12-07 东阿阿胶股份有限公司 Application of donkey-hide gelatin in preparation of cell culture for promoting cell division propagation and resisting apoptosis
CN107752001A (en) * 2017-10-15 2018-03-06 长春中医药大学 A kind of ginseng small molecule oral liquid and preparation method thereof
CN108077503A (en) * 2017-12-21 2018-05-29 湖南光虹健康管理有限公司 A kind of composition containing ginseng donkey-hide gelatin and preparation method thereof

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