CN107890542A - Treat the Chinese medicine composition and its preparation method of diarrhoea - Google Patents
Treat the Chinese medicine composition and its preparation method of diarrhoea Download PDFInfo
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- CN107890542A CN107890542A CN201711432779.4A CN201711432779A CN107890542A CN 107890542 A CN107890542 A CN 107890542A CN 201711432779 A CN201711432779 A CN 201711432779A CN 107890542 A CN107890542 A CN 107890542A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9064—Amomum, e.g. round cardamom
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8994—Coix (Job's tears)
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- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The present invention relates to the Chinese medicine composition and its preparation method for the treatment of diarrhoea.Specifically, the integration of drinking and medicinal herbs Chinese medicine composition for the treatment of diarrhoea includes purslane, Poria cocos, coix seed, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizae.The present composition uses food and medicament dual-purpose composition completely, and security is higher, and curative effect is clear and definite, reduces the toxic side effect for enteritis of being suffered from diarrhoea in the prior art with antibiotic or other traditional Chinese medicine composition for treating.The preparation method of integration of drinking and medicinal herbs composition is also provided, main active purslane uses microwave radiation exaraction, then using water as solvent extraction, finally removes impurity in the method for alcohol precipitation, the purslane extract active constituent content of acquisition is high, improves anti-diarrhea curative effect；The microwave auxiliary extraction technology of application simultaneously, extraction process is simple, and extraction time is short, is advantageous to industrial production.
Description
Technical field
The invention belongs to Chinese medicine composition and Chinese medical extract field, and in particular to a kind of integration of drinking and medicinal herbs for including purslane
Composition；A kind of preparation method of purslane extract is directed to, said composition is used to treat enteritis or diarrhoea.
Background technology
In recent years, as people's living standard generally improves, dietetic hygiene and nutrient knowledge gradually popularization and the public
Health perception increasingly strengthens, and people increasingly pay attention to health care and health, advocates nature.In western countries, it is thus proposed that use " kitchen
Room replaces pharmacy ", " food alternatives to medication "." integration of drinking and medicinal herbs " thought of Chinese tradition is the reflection of food health care thought, bag
Contain the contents such as dietotherapy, health care and the herbal cuisine in traditional Chinese medicine and pharmacy.
" integration of drinking and medicinal herbs " is primarily referred to as food and herbal medicine same source.The traditional medicine and cooking culture in China all have medicine food
The saying of homologous, medicine food similarly, this is that our ancestors sum up the experience herbal cuisine taken in historical floods, and dietotherapy not only can be with
Health-preserving and disease-preventing, and there is auxiliary therapeutic action to a variety of common diseases." integration of drinking and medicinal herbs " is the important of the magnificent culture of the Chinese nation
One of crystallization, and health food idea earliest in the world.What the Ministry of Public Health announced《On further specification healthy food material
The notice of management》In, it is specified that 84 kinds of articles such as cloves, anise, fennel are to be article list that food is again medicine.
Purslane (Portulaca oleracea L.) is Portulacaceae Herba portulacae platymiscium, also known as HERBA PORTULACAE, the five-element
Grass, long life dish, peristrophe etc., China north and south various regions are produced, and blazon whole world temperate zone and torrid areas, are planted for medicine-food two-purpose
Thing.According to《Compendium of Materia Medica》Record, can be clinically used for " dissipating blood detumescence, sharp intestines habitual abortion, detoxify treating stranguria, abnormal sweating after managing property ".Purslane
Containing abundant aliphatic acid, volatile oil, polysaccharide, a variety of chemical compositions with medical value such as amino acid and mineral matter, this makes
The exploitation for obtaining purslane has good material base.
Widely distributed purslane is a kind of annual herb plant in worldwide, existing 1,100 in China
Edible history.Scientific research in recent years confirms that purslane is both a kind of food, has great medical value again, it has also become state
One of integration of drinking and medicinal herbs wild plant that the Ministry of Public Health of family is assert.Purslane herb can be used as medicine, and its is sour cold in nature, has no toxic side effect, tool
There are clearing heat and detoxicating, radiating detumescence, QI invigorating ease constipation.Now think that it can promote epithelial cell function to tend to be normal, accelerate to burst
Ulcer hinders the healing in face, while has good efficacy to treatment acute gastroenteritis, dysentery, urinary system infection contamination etc..Purslane also contains
There are glutamic acid, aspartic acid, alanine, glucose, fructose, a variety of nutriments such as sucrose and a large amount of sylvite.In addition, dent
Amaranth contains flavonoids, alkaloid, phenols, a variety of chemical compositions such as organic acid and terpene.Basic research shows that purslane can be significantly
The activity of small entero-amylase and protease is improved, improves the stool of Ulcerative Colitis Model rat, reduces peroxide
Enzyme content, mitigate intestinal mucosal injury and intestinal inflammatory.
Enteritis is that enteritis and colitis, clinical manifestation mainly have abdomen caused by bacterium, virus, fungi and parasite etc.
Bitterly, just or mucus purulence blood, some patients can have heating and a tenesmus sensation, therefore also known as infectious diarrhea for diarrhoea, dilute water.Enteritis
It is different by course of disease length, it is divided into acute and chronic two class, typically more than two months, clinic common are slowly the chronic enteritis course of disease
Property bacillary dysentery, chronic amebic dysentery, snail fever, nonspecific ulcerative colitis and limit office property enteritis etc..Diarrhoea
It can be caused by Different types of etiopathogenises, thought from the angle of the traditional Chinese medical science, it is interior because weakness of the spleen and the stomach, it is outer because diet is without section or zone of eclipse bacterium or raw food
Thing caused by.Treatment diarrhoea, western medicine therapy is more using antibiotic, infusion treatment, and therapy course for the treatment of length, medical expense is high, many
Patient cannot treat because of medical expense beyond one's means.Therefore, for cheap, short treating period, abdomen is safely and effectively treated
There is urgent demand for the medicine rushed down.
It is used to treat enteritis or suffer from diarrhoea have many places record for purslane in the prior art.As CN101259245A is (open
Day：20080910) describe a kind of Chinese medicine composition for including purslane, have gut purge dampness elimination, nourishing the kidney and the liver, vital energy regualting and blood circulation-promoting,
Acted on by stasis of blood antidiarrheal etc., for the disease such as stomachache, diarrhoea, tenesmus, mucopurulent bloody stool caused by ulcerative colitis.
And for example CN1544058A (publication date：20041110) describe and a kind of to treat chronic enteritis, colitis, rectitis
Chinese medicine preparation, comprising purslane, catechu etc., through medicament made of traditional processing procedure.The card that this medicine is led is diarrhoea, abdomen
Bitterly, abdominal distension, tenesmus, the red white purulence blood of diarrhea.Gone from clearing heat and detoxicating, intestines strengthening and diarrhea checking, excreting dampness stop dysentery, myogenic rotten vertical to treat
Method, above-mentioned each all medicines of taste complement each other, and there is clearing heat and detoxicating, intestines strengthening and diarrhea checking, excreting dampness stop dysentery, myogenic to go rotten function, suitable for controlling
Treat the retention of damp-heat in the interior type of stomachache, diarrhoea, tenesmus, pus and blood stool or bloody stool category differential diagnosis in tcm.Preparation is made up of pure Chinese herbal medicine, mark
Originally it is simultaneous to control, it is evident in efficacy, have no adverse reaction, have no toxic side effect, clinical symptom disappearance is rapid, without rebound phenomena after healing.
The preparation method on purslane extract also has many places record in the prior art.As CN101181317A is (open
Day：20080521) a kind of extract prepared using Portulaca oleracea L as raw material and extracting method are described, and by the extract
For preparing anti anoxia, antifatigue, resisting stress medicine and food.Conventional purslane extract is using after water or alcohol extracting
Supernatant is taken, caused precipitation is centrifuged and is abandoned as invalid components.The present invention is it has been investigated that Portulaca water or alcohol extract are fitted
After concentration, sediment caused by centrifugation has significant anti anoxia, antifatigue, anti-stress effect on the contrary.The invention provides
A kind of preparation method of new purslane extract, it is preferable that purslane extract made from this method the experiment proved that it has
Anti anoxia, antifatigue, anti-stress effect.
And for example CN1569127A (publication date：20050126) describe and answered with purslane extract with what ginsenoside formed
Square preparation.Purslane extract first is prepared with water extraction and alcohol precipitation method, then purslane extract is matched somebody with somebody by a certain percentage with ginsenoside
5 are made compound preparation.Through zoopery, it was demonstrated that have significant oxygen lack resistant function.Preparation method of the present invention is simple, and cost is low
It is honest and clean, available for preparing anti-anoxic medicine or food.
And for example CN102219669A (publication date：20111019) describe one kind and use supercritical carbon dioxide extraction dent
The linolenic method of amaranth, comprises the following steps：The purslane after appropriate crush is taken, loads supercritical carbon dioxide extraction system
Extraction column after seal, extracting system is heated up respectively, boosts to extraction conditions, starts the cycle over extraction, and adjust CO2 stream
Amount, kept for constant temperature and pressure extraction certain time.The present invention extracts α-Asia with supercritical carbon dioxide extraction technology from purslane
Numb acid, extraction process are carried out under the low temperature no more than 60 DEG C, up to 40Mpa or so high pressure, effectively reduce unsaturation
The oxidation deterioration of aliphatic acid, makes CO2 deeply penetrate into inside plant cell, obtains higher output capacity.With traditional extraction process
Compare, the efficiency of pcr product that this method obtains is high, noresidue solvent, greatly improves product quality, and substantially reduce extraction
Time.
The present invention provides a kind of food and medicine consangunity Chinese medicine composition comprising purslane, composition comprising purslane, Poria cocos,
Coix seed, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizae；Food and medicine consangunity Chinese medicine composition of the present invention can also be further
Include the membrane of a chicken's gizzard, the root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel.The present composition using food and medicament dual-purpose plant purslane as chief active into
Point, add the preparation such as other food and medicine consangunity composition Poria cocos, coix seed, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizaes, place
Fang Zucheng uses food and medicament dual-purpose composition completely, therefore security is higher, and curative effect is clear and definite, reduce in the prior art with antibiotic or
The toxic side effect of other traditional Chinese medicine composition for treating diarrhoea enteritis.
Present invention provides a kind of preparation method of purslane extract.In the prior art for the extraction side of purslane
The composition that method has not focused on purslane extract is extremely complex, and some invalid impurity component contents are too high, is follow-up
Preparation R＆D work brings more highly difficult.The present invention is solution above the deficiencies in the prior art, there is provided a kind of purslane
Extracting method, the inventive method use microwave radiation exaraction first, then using water as solvent extraction, are finally removed in the method for alcohol precipitation
Decontamination, the purslane extract active constituent content of acquisition is high, improves anti-diarrhea curative effect；The microwave radiation technology of application simultaneously
Extractive technique, extraction process is simple, and extraction time is short, is advantageous to industrial production.
The content of the invention
The problem of existing for prior art, the present invention provide a kind of integration of drinking and medicinal herbs Chinese medicine composition for treating diarrhoea, bag
Containing purslane, Poria cocos, coix seed, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizae；Combination of the above thing can also be further
Include the food and medicine consangunity traditional Chinese medicinal components the membrane of a chicken's gizzard, the root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel.The present composition is used to treat diarrhoea enteritis,
Composition uses food and medicament dual-purpose material, its security and validity better than other similar preparations in the prior art completely.
The content proportioning of the present composition can be purslane 60-100, Poria cocos 60-80, coix seed 60-80, Chinese yam
10-20, Semen Lablab Album 10-20, lotus seeds 10-20, fructus amomi 10-20, radix platycodi 10-20 and radix glycyrrhizae 10-20；Or the present composition enters
One step includes the membrane of a chicken's gizzard 10-20, root of kudzu vine 10-20, Radix Codonopsis 10-20, hawthorn 10-20 and dried orange peel 10-20.
The present invention also provides a kind of preparation method of purslane extract, and comprising being extracted with microwave assisted techniques, extraction is warm
40-50 DEG C of degree, Extraction solvent are acetone or methanol, solid-liquid ratio 1：25 to 1：50, obtain extract solution after extracting 3-5 minutes；Subtract
Pressure revolving removes acetone in extract solution and, to obtain extract medicinal extract, adds water dissolving medicinal extract, removing is heavy after centrifuging 15-30 minutes
Form sediment；Collect 10-20 times of supernatant addition to measure ethanol and stir, stand 12-24 hours under room temperature condition, cross and filter out precipitation,
Obtain supernatant；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, produce dent and look for and carry
Take thing.
Wherein, the solid-liquid ratio of purslane and acetone can be 1:25；
Wherein, microwave radiation exaraction temperature can be 40 DEG C；
Meanwhile it can add 10 times of amount ethanol after collection supernatant in method made above and be staticly settled；Make above
The mass concentration that ethanol is added in Preparation Method is 70%.
Specifically, the present invention provides following technical scheme：
A kind of integration of drinking and medicinal herbs Chinese medicine composition for treating diarrhoea of project 1., it is characterised in that include purslane, Poria cocos, Job's tears
Benevolence, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizae.
Chinese medicine composition of the project 2. as described in project 1, it is characterised in that include purslane 60-100, Poria cocos 60-80, the heart of a lotus seed
Seed of jog's tears 60-80, Chinese yam 10-20, Semen Lablab Album 10-20, lotus seeds 10-20, fructus amomi 10-20, radix platycodi 10-20 and radix glycyrrhizae 10-20.
Chinese medicine composition of the project 3. as described in project 1, it is characterised in that further comprising integration of drinking and medicinal herbs traditional Chinese medicinal components chicken
Interior gold, the root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel.
Chinese medicine composition of the project 4. as described in project 3, it is characterised in that include purslane 60-100, Poria cocos 60-80, the heart of a lotus seed
Seed of jog's tears 60-80, Chinese yam 10-20, Semen Lablab Album 10-20, lotus seeds 10-20, fructus amomi 10-20, radix platycodi 10-20, radix glycyrrhizae 10-20, in chicken
Golden 10-20, root of kudzu vine 10-20, Radix Codonopsis 10-20, hawthorn 10-20 and dried orange peel 10-20.
A kind of preparation method of the composition as described in project 1 of project 5., it is characterised in that comprise the steps of：
Step 1：Purslane is extracted with microwave assisted techniques, 40 DEG C of Extracting temperature, Extraction solvent is acetone or methanol, feed liquid
Than for 1：25, obtain extract solution after extracting 3-5 minutes；Vacuum rotary steam removes in extract solution acetone to obtain extract medicinal extract, then
Be dissolved in water medicinal extract, and precipitation is removed after centrifuging 15-30 minutes；Collect supernatant to add 10 times of amount ethanol and stir, room temperature
Under the conditions of stand 12-24 hours, cross and filter out precipitation, acquisition supernatant；Continue supernatant rotary evaporation removing ethanol, finally will
The medicinal extract of acquisition is dried, and produces dent and looks for extract；
Step 2：Raw material is weighed by each raw material proportioning, wherein coix seed, Chinese yam, lotus seeds are ground into coarse powder, with 75% ethanol
Refluxing extraction three times, merges extract solution, filters, and filtrate is standby；Fructus amomi extracts volatile oil with steam distillation, and distillate is standby；
Coix seed, Chinese yam, the dregs of a decoction and remaining Poria cocos, Semen Lablab Album, radix platycodi, radix glycyrrhizae, the membrane of a chicken's gizzard, the root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel of lotus seeds
Add water to cook twice；Mixed liquor is obtained after merging the extract solution of above decocting liquid, fructus amomi distillate and coix seed etc.；With 75% ethanol
The dent prepared in dissolving step 1 looks for extract powdered extract, adds in liquid mixed above；Continue plus ethanol reaches alcohol content
75%, 24 hours are stood, filtration, it is 1.05-1.10 that filtrate decompression, which is concentrated into density, is dried, both.
A kind of 6. dent of project looks for the preparation method of extract, it is characterised in that purslane is extracted with microwave assisted techniques,
40-50 DEG C of Extracting temperature, Extraction solvent are acetone, solid-liquid ratio 1：25 to 1：50, obtain extract solution after extracting 3-5 minutes；Subtract
Pressure revolving removes acetone in extract solution and, to obtain extract medicinal extract, adds water dissolving medicinal extract, removing is heavy after centrifuging 15-30 minutes
Form sediment；Collect 10-20 times of supernatant addition to measure ethanol and stir, stand 12-24 hours under room temperature condition, cross and filter out precipitation,
Obtain supernatant；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, produce dent and look for and carry
Take thing.
Preparation method of the project 7. as described in project 6, it is characterised in that solid-liquid ratio 1:25.
Preparation method of the project 8. as described in project 6, it is characterised in that microwave radiation exaraction temperature is 40 DEG C.
Preparation method of the project 9. as described in project 6, it is characterised in that add 10 times of amount ethanol progress after collecting supernatant
Staticly settle.
Preparation method of the project 10. as described in project 6, it is characterised in that the mass concentration for adding ethanol is 70%.
The method that the present invention prepares purslane extract has the characteristics of excellent, such as wherein flavonoid content is high,
Such as in 1~embodiment of embodiment 3, three kinds of purslane extracts of gained, Quercetin, Kaempferol, cyanidenon, celery after measured
Plain four kinds of chromocor compound contents respectively reach 11.3~15.2%, 3.6~5.1%, 6.4~8.7%, 9.7~14.1% models
Enclose, and these purslane extracts or excellent biology effect is presented including its composition, such as implement in the present invention
Described in example 6.In addition, the stability of medicine is the important step and quality evaluation index of Drug's control, send out
Existing, the gained purslane extract of 1~embodiment of the embodiment of the present invention 3 and the resulting composition of 5~embodiment of embodiment 6 are undergoing
After the classical stability test processing that June is placed under the conditions of 40 DEG C of lucifuges, above-mentioned four kinds of flavones in these extracts or composition
Class compound is down to 90~92% scope, and by preparing purslane extract in the present invention during carry out special place
During reason, the stability of above-mentioned four kinds of flavone compounds in extract obtained or composition can be significantly improved.Specific situation is,
Have now surprisingly been found that, into the gained extract solution after microwave assisted techniques extract 3~5 minutes, add a small amount of white bole
Combined with both propane diols, can significantly make above-mentioned typical activity chemical substance (Quercetin, Kaempferol, cyanidenon, celery
Element) it is able to be enriched with extract of the present invention, and particularly can make four kinds of flavonoids in extract obtained or composition
Significantly more excellent stability is presented during extract of the present invention preserves for a long time in compound.Because white bole is a kind of conventional in itself
Pharmaceutic adjuvant and its it is not soluble in water be also insoluble in ethanol while be also insoluble in acetone, therefore pass through letter in the methods of the invention
Single subsequent treatment means can be cleared by its；And propane diols is because its addition is small, and it is a kind of extremely safe and conventional
Pharmaceutic adjuvant, even if it has a remnants in extract of the present invention, this small amount of remnants will not cause any to body completely
Detrimental effect.For example, in the first complementary testing of the present invention, the method that respectively refers to embodiment 1-3, different be only to
Through microwave assisted techniques extract 3~5 minutes after gained extract solution in add white bole (in an amount of from the 0.5% of extract solution liquid measure) and
Propane diols (in an amount of from the 0.25% of extraction liquid measure), stirs, then carries out follow-up vacuum rotary steam process；This first supplement
Three kinds of extracts of experiment gained after testing, wherein compared to it join by Quercetin, Kaempferol, cyanidenon, apiolin content
It is significant for the embodiment 1~3 examined to improve 3~5 percentage points, because the content of four kinds of flavones in embodiment 1~3 is absolute
Amount is not high, by that can improve 3~5 percentage points after this first complementary testing method processing there is extremely important practice to anticipate
Justice.In the second complementary testing of the present invention, the method that respectively refers to the first complementary testing, different is not added in extract solution
Add white bole and only plus propane diols, three batches of extracts of gained after testing, wherein Quercetin, Kaempferol, cyanidenon, apiolin four
Person's content, without significantly improving or even having reduction, is differed -0.22~0.38 for its embodiment referred to 1~3
In the range of percentage point.In the 3rd complementary testing of the present invention, the method that respectively refers to the first complementary testing, different is only
Do not add propane diols in extract solution and only plus white bole, three batches of extracts of gained after testing, wherein Quercetin, Kaempferol, sweet-scented osmanthus
Without significantly improving or even having reduction for its embodiment referred to 1~3, difference is equal for careless element, apiolin content
In the range of -0.18~0.31 percentage point.In addition, in the 4th complementary testing of the present invention, will be various obtained by the present invention
Purslane extract or embodiment 5 and the resulting composition of embodiment 6 seal respectively, are placed under the conditions of 40 DEG C of lucifuges and place June, survey
Quercetin, Kaempferol, cyanidenon, the respective content of apiolin in each extract when fixed 0 month and during June, for a certain
Four kinds of chemical substances in extract or composition, respectively with content in its divided by 0 month content multiplied by with the percentage of 100% gained in June
Number, remaining percentage amounts of certain flavonoid substance after being disposed through this 40 DEG C-June in the extract or composition during as June, this is residual
Remaining percentage amounts show that the stability of the flavonoid substance in the extract or composition is better closer to 100%, lead in pharmaceutical field
It is necessary that normal remaining percentage amounts, which are more than 90%, is preferable more than 95%.After measured, the gained of the embodiment of the present invention 1~3 three batches
Three batches of extracts, three batches of extracts of the 3rd complementary testing gained, embodiment 5~6 two batch group obtained by extract, the second complementary testing
In compound, Quercetin, Kaempferol, cyanidenon, apiolin remaining percentage amounts respectively in the range of 90.2~91.4%,
In the range of 90.8~91.4%, in the range of 91.1~91.9%, in the range of 90.2~91.6%, show in these extracts four kinds
The stability of chemical substance can still meet in general technical requirements；Three batches of purslane extracts obtained by first complementary testing of the invention
In, Quercetin, Kaempferol, cyanidenon, the remaining percentage amounts of apiolin are respectively in the range of 99.3~99.9%, 98.9
In the range of~99.5%, in the range of 99.0~99.4%, in the range of 99.1~99.6%, show four kinds of flavones in these extracts
The stability of compound significantly improves.Generally speaking, change the technique of method for preparing extractive and may change in extract and change
Content of material is learned, this situation will be understood by, but the process conditions for changing extract in the present invention can also be notable
The stability of material in extract is improved, this is that prior art can not be predicted completely.Therefore, in purslane extract of the present invention
Preparation method an embodiment in, to through microwave assisted techniques extract 3~5 minutes after gained extract solution in add white pottery
Soil is (in an amount of from 0.2~1% (g/100ml) of extract solution liquid measure, such as 0.5%) with propane diols (in an amount of from the 0.2 of extraction liquid measure
~0.5% (g/100ml), such as 0.25%), stir, then carry out the vacuum rotary steam.In other complementary testing
In, it has been found that reference embodiment 1 is extract obtained in the first complementary testing of the invention, its side according to the embodiment of the present invention 6
Method is tested, and its anti diar rhea experimental result is significantly more excellent than medicine 1, shows that this extract is not only obtained by the first complementary testing
Biological activity is stronger and has more preferable chemical stability.
Embodiment
Illustrate the present invention referring to specific embodiment.It will be appreciated by those skilled in the art that these embodiments are only
For illustrating the present invention, its scope not limiting the invention in any way.
Raw material, reagent in following embodiments, including medicinal material etc., unless otherwise specified, it is purchased from disclosed commercial channel.
Test method used in following embodiments and test example, if not otherwise specified, using the conventional method of this area.
Test example 1：The high-performance liquid chromatogram determination method of chromocor compound in purslane
When being measured to purslane typical case's chromocor compound in the various extracts of the present invention and composition, using this experiment
The method of example 1 is carried out.
The typical chromocor compound included in purslane has Quercetin, Kaempferol, cyanidenon, apiolin etc..
Chromocor compound (determining typical Quercetin, Kaempferol, cyanidenon, apiolin) is efficient in purslane
Liquid chromatogram measuring method, with reference to Wang Jin armies document (Wang Jinjun etc., the high performance liquid chromatography of chromocor compound point in purslane
Analysis, PLA's Acta Pharmaceutica Sinica, 04 phase in 2010) method progress, chromatographic condition：Chromatographic column ZORBAXSB-C18 (150mm ×
4.6mm, 5 μm), Detection wavelength 360nm, the phosphoric acid of methanol -0.2% (45: 55) is mobile phase, 30 DEG C of column temperature, flow velocity 1.0ml/
min。
Embodiment 1：The preparation of purslane extract
Extracted with microwave assisted techniques, 45 DEG C of Extracting temperature, Extraction solvent is acetone, solid-liquid ratio 1: 35, is extracted 4 minutes
After obtain extract solution；Vacuum rotary steam removes acetone in extract solution and, to obtain extract medicinal extract, adds water dissolving medicinal extract, centrifuge 20 points
Precipitation is removed after clock；Collect 15 times of amount ethanol of supernatant addition simultaneously to stir, stand 18 hours under room temperature condition, it is heavy to filter out excessively
Form sediment, obtain supernatant；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, produces dent
Look for extract.
Embodiment 2：The preparation of purslane extract
Extracted with microwave assisted techniques, 40 DEG C of Extracting temperature, Extraction solvent is acetone, solid-liquid ratio 1：25, extract 5 minutes
After obtain extract solution；Vacuum rotary steam removes acetone in extract solution and, to obtain extract medicinal extract, adds water dissolving medicinal extract, centrifuge 30 points
Precipitation is removed after clock；Collect 10 times of amount ethanol of supernatant addition simultaneously to stir, stand 12 hours under room temperature condition, it is heavy to filter out excessively
Form sediment, obtain supernatant；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, produces dent
Look for extract.
Embodiment 3：The preparation of purslane extract
Extracted with microwave assisted techniques, 50 DEG C of Extracting temperature, Extraction solvent is acetone, solid-liquid ratio 1: 50, is extracted 3 minutes
After obtain extract solution；Vacuum rotary steam removes acetone in extract solution and, to obtain extract medicinal extract, adds water dissolving medicinal extract, centrifuge 15 points
Precipitation is removed after clock；Collect 20 times of amount ethanol of supernatant addition simultaneously to stir, stand 24 hours under room temperature condition, it is heavy to filter out excessively
Form sediment, obtain supernatant；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, produces dent
Look for extract.
Embodiment 4：Integration of drinking and medicinal herbs Chinese medicine composition
A kind of integration of drinking and medicinal herbs Chinese medicine composition, is made up of the medicinal material of following parts by weight：Purslane 80, Poria cocos 70, coix seed
70th, Chinese yam 15, Semen Lablab Album 15, lotus seeds 15, fructus amomi 15, radix platycodi 15 and radix glycyrrhizae 15.
Preparation method：Raw material is weighed by each raw material proportioning, wherein coix seed, Chinese yam, lotus seeds are ground into coarse powder, with 75% second
Alcohol reflux extracts three times, merges extract solution, filters, and filtrate is standby；Fructus amomi extracts volatile oil with steam distillation, and distillate is standby
With；Coix seed, Chinese yam, the dregs of a decoction of lotus seeds and remaining Poria cocos, Semen Lablab Album, radix platycodi and radix glycyrrhizae add water to cook twice；Decocted more than merging
Mixed liquor is obtained after the extract solution of liquid, fructus amomi distillate and coix seed etc.；Looked for the dent that the dissolving of 75% ethanol is implemented to prepare in 1
Extract powdered extract, add in liquid mixed above；Continue plus ethanol makes alcohol content stand 24 hours up to 75%, filter, filtrate
It is 1.05-1.10 to be concentrated under reduced pressure into density, is dried, both.
Embodiment 5：Integration of drinking and medicinal herbs Chinese medicine composition
A kind of integration of drinking and medicinal herbs Chinese medicine composition, is made up of the medicinal material of following parts by weight：Purslane 80, Poria cocos 70, coix seed
70th, Chinese yam 15, Semen Lablab Album 15, lotus seeds 15, fructus amomi 15, radix platycodi 15 and radix glycyrrhizae 15, the membrane of a chicken's gizzard 15, the root of kudzu vine 15, Radix Codonopsis 15, hawthorn 15
With dried orange peel 15.
Preparation method：Raw material is weighed by each raw material proportioning, wherein coix seed, Chinese yam, lotus seeds are ground into coarse powder, with 75% second
Alcohol reflux extracts three times, merges extract solution, filters, and filtrate is standby；Fructus amomi extracts volatile oil with steam distillation, and distillate is standby
With；Coix seed, Chinese yam, the dregs of a decoction of lotus seeds and remaining Poria cocos, Semen Lablab Album, radix platycodi, radix glycyrrhizae, the membrane of a chicken's gizzard, the root of kudzu vine, Radix Codonopsis, hawthorn and
Dried orange peel adds water to cook twice；Mixed liquor is obtained after merging the extract solution of above decocting liquid, fructus amomi distillate and coix seed etc.；With 75%
The dent that ethanol dissolving is implemented to prepare in 1 looks for extract powdered extract, adds in liquid mixed above；Continue plus ethanol makes alcohol content
Up to 75%, 24 hours are stood, filtration, it is 1.05-1.10 that filtrate decompression, which is concentrated into density, is dried, and is both obtained.
Embodiment 6：Purslane extract and the anti-CaM antibody research comprising purslane extract composition
Experimental animal：Male mice in kunming (20 ± 2g of body weight) 60 is selected, is randomly divided into 6 groups.
Experimental agents select：
1. control 1：Carboxymethylcellulose sodium solution
2. control 2：Smecta group (anti-diarrheal)
3. control 3：Purslane extract, prepared according to method in CN1569127A (by purslane herb or cauline leaf dry product
Flowed back 2 hours with petroleum ether, volatilize solvent, purslane is decocted 2 times with distilled water, is for the first time 2 hours, it is small for 1 second
When 30 minutes, merge decoction liquor, with 2500~3000 revs/min centrifuge 20~30 minutes, abandon precipitation, supernatant puts 100 DEG C of water
After bath concentration, it is 80% to add ethanol to determining alcohol, stirs evenly, 24~48 hours are stood under the conditions of 5~10 DEG C, is filtered, by filtrate
It is concentrated and dried, obtains purslane extract).
4. medicine 1：The purslane extract group of embodiment 1
5. medicine 2：The composition group of embodiment 4
6. medicine 3：The composition group of embodiment 5
It was 0.5% carboxymethylcellulose sodium solution to compare 1 group, with the solution continuous gavage 5 days；Remaining each group with
0.5% carboxymethylcellulose sodium solution, which dissolves each relative medicine, to be prepared, and ensures drug concentration 0.03g/ml；Equally with each group medicine
Solution continuous gavage 5 days, dosage are by mouse weight 0.04ml/g；After last dose 1h, by 2g/kg dosage, give
Every mouse of each group fills folium sennae suspension 0.03mL/g once, to changing blotting paper per hour 1 time in 1~4h after folium sennae liquid,
Calculate muck sum in each group 4h.Muck number using every or per heap as 1 time, with muck number number represent diarrhoea degree.
Table 1：Anti diar rhea experimental result
Upper table shows that compared with compareing 1-3, purslane extract or pharmaceutical composition are respectively provided with substantially in medicine group 1-3
Anti diarrhea activity；The anti diarrhea activity of the purslane extract prepared in the process of the present invention is prepared also superior to art methods
Purslane extract.Experiment is easily drawn a conclusion more than, and the present composition uses food and medicament dual-purpose composition, security completely
Higher, curative effect is clear and definite, reduces the toxic side effect for enteritis of being suffered from diarrhoea in the prior art with antibiotic or other traditional Chinese medicine composition for treating.
Purslane extract and treating diarrhoea or enteritis field comprising purslane extract integration of drinking and medicinal herbs composition prepared by the present invention
Have wide practical use.
Those of ordinary skills in the art should understand that：The discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under the thinking of the present invention, above example
Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and exist such as
Many other changes of upper described different aspect of the invention, for simplicity, they are not provided in details.Therefore, it is all
Within the spirit and principles in the present invention, any omission for being made, modification, equivalent substitution, improvement etc., it should be included in the present invention's
Within protection domain.
Claims (10)
- A kind of 1. integration of drinking and medicinal herbs Chinese medicine composition for treating diarrhoea, it is characterised in that comprising purslane, Poria cocos, coix seed, Chinese yam, Semen Lablab Album, lotus seeds, fructus amomi, radix platycodi and radix glycyrrhizae；Such as its method as claimed in claim 5 is prepared.
- 2. Chinese medicine composition as claimed in claim 1, it is characterised in that include purslane 60-100, Poria cocos 60-80, coix seed 60-80, Chinese yam 10-20, Semen Lablab Album 10-20, lotus seeds 10-20, fructus amomi 10-20, radix platycodi 10-20 and radix glycyrrhizae 10-20.
- 3. Chinese medicine composition as claimed in claim 1, it is characterised in that further comprising the integration of drinking and medicinal herbs traditional Chinese medicinal components the membrane of a chicken's gizzard, The root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel.
- 4. Chinese medicine composition as claimed in claim 3, it is characterised in that include purslane 60-100, Poria cocos 60-80, coix seed 60-80, Chinese yam 10-20, Semen Lablab Album 10-20, lotus seeds 10-20, fructus amomi 10-20, radix platycodi 10-20, radix glycyrrhizae 10-20, the membrane of a chicken's gizzard 10- 20th, root of kudzu vine 10-20, Radix Codonopsis 10-20, hawthorn 10-20 and dried orange peel 10-20.
- 5. a kind of preparation method of composition as claimed in claim 1, it is characterised in that comprise the steps of：Step 1：Purslane is extracted with microwave assisted techniques, 40 DEG C of Extracting temperature, Extraction solvent is acetone or methanol, and solid-liquid ratio is 1：25, extract obtain after 3-5 minutes extract solution (it is optional, to the gained extract solution after microwave assisted techniques extract 3~5 minutes 0.5%) and propane diols is (in an amount of from carrying middle addition white bole is (in an amount of from 0.2~1% (g/100ml) of extract solution liquid measure, such as 0.2~0.5% (g/100ml) of liquid measure is taken, such as 0.25%), is stirred)；Vacuum rotary steam remove extract solution in acetone with Extract medicinal extract is obtained, adds water dissolving medicinal extract, precipitation is removed after centrifuging 15-30 minutes；Collect supernatant and add 10 times of amount second Alcohol is simultaneously stirred, and 12-24 hours are stood under room temperature condition, is crossed and is filtered out precipitation, obtains supernatant；Continue to rotate supernatant and steam Hair removes ethanol, and finally the medicinal extract of acquisition is dried, produces dent and looks for extract；Step 2：Raw material is weighed by each raw material proportioning, wherein coix seed, Chinese yam, lotus seeds are ground into coarse powder, with 75% alcohol reflux Extraction three times, merges extract solution, filters, and filtrate is standby；Fructus amomi extracts volatile oil with steam distillation, and distillate is standby；Job's tears Benevolence, Chinese yam, the dregs of a decoction of lotus seeds and remaining Poria cocos, Semen Lablab Album, radix platycodi, radix glycyrrhizae, the membrane of a chicken's gizzard, the root of kudzu vine, Radix Codonopsis, hawthorn and dried orange peel add water Decoct twice；Mixed liquor is obtained after merging the extract solution of above decocting liquid, fructus amomi distillate and coix seed etc.；Dissolved with 75% ethanol The dent prepared in step 1 looks for extract powdered extract, adds in liquid mixed above；Continue plus ethanol make alcohol content up to 75%, 24 hours are stood, filtration, it is 1.05-1.10 that filtrate decompression, which is concentrated into density, is dried, both.
- 6. a kind of dent looks for the preparation method of extract, it is characterised in that extracts purslane, Extracting temperature with microwave assisted techniques 40-50 DEG C, Extraction solvent is acetone, solid-liquid ratio 1：25 to 1：50, obtain extract solution after extracting 3-5 minutes；Vacuum rotary steam removes Acetone in extract solution is gone to obtain extract medicinal extract, to add water dissolving medicinal extract, precipitation is removed after centrifuging 15-30 minutes；In collection Clear liquid adds 10-20 times and measures ethanol and stir, and 12-24 hours are stood under room temperature condition, crosses and filters out precipitation, obtains supernatant Liquid；Continue supernatant rotary evaporation removing ethanol, finally the medicinal extract of acquisition is dried, dent is produced and looks for extract.
- 7. preparation method as claimed in claim 6, it is characterised in that：Solid-liquid ratio is 1:25；And/or to through microwave radiation technology skill White bole is added (in an amount of from 0.2~1% (g/100ml) of extract solution liquid measure, example in gained extract solution after art is extracted 3~5 minutes As 0.5%) (in an amount of from 0.2~0.5% (g/100ml) of extraction liquid measure, such as 0.25%), stirred, connecing with propane diols And carry out the vacuum rotary steam.
- 8. preparation method as claimed in claim 6, it is characterised in that microwave radiation exaraction temperature is 40 DEG C.
- 9. preparation method as claimed in claim 6, it is characterised in that 10 times of amount ethanol of addition are stood after collecting supernatant Precipitation.
- 10. preparation method as claimed in claim 6, it is characterised in that the mass concentration for adding ethanol is 70%.
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