CN106727762A - The natural component extracting method of purslane - Google Patents
The natural component extracting method of purslane Download PDFInfo
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- CN106727762A CN106727762A CN201611241864.8A CN201611241864A CN106727762A CN 106727762 A CN106727762 A CN 106727762A CN 201611241864 A CN201611241864 A CN 201611241864A CN 106727762 A CN106727762 A CN 106727762A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of natural component extracting method of purslane, comprise the following steps：Step (1) crushing, the alcohol extracting of step (2) impulse ultrasound waves, step (3) secondary alcohol extracting, step (4) concentration, step (5) alcohol precipitation and step (6) concentrate drying.In the present invention, as being crushed to purslane, the alcohol extracting of impulse ultrasound waves, secondary alcohol extracting, concentration, the natural component of alcohol precipitation and purslane obtained by the step of concentrate drying, natural (property of medicine) composition of purslane is remained to the full extent, reduce the loss of natural (property of medicine) composition of purslane, and the natural component of the purslane has the function of improving body immunity, can be effectively used for treating the low symptom of body immunity, mitigate the pain of body immunity immunocompromised patients.And because the natural component of the purslane is powdered, it is easy to different consumer groups to carry and use.
Description
Technical field
The present invention relates to constituents extraction field, more particularly, to the natural component extracting method of purslane.
Background technology
Purslane (scientific name：Portulaca oleracea L.) annual herb, complete stool is without hair.Stem prostration, paving of throwing oneself on the ground
Dissipate, branch light green or band kermesinus.Leaf alternate, blade is flat, plump, like dent shape, above dirty-green, below light green or band
Kermesinus；Petiole tubbiness.Flower is bloomed without stalk, the period of the day from 11 a.m. to 1 p.m；Bract is lobate；Sepal green, helmet shape；Petal yellow, obovate；Pistillate flower
Medicine yellow；Ovary is without hair.Capsule ovoid；Seed is tiny, and deflection is spherical, and dark brown is glossy.The month at florescence 5-8, fruiting period 6-9
Month.
Purslane popular name longevity greens/mustard green, is a kind of relatively common herbaceous plant, wherein rich in flavones, polysaccharide and vitamin
C, vitamin E etc., with multiple efficacies such as enhance immunity, hypoglycemic, anti-inflammation, anti-aging, diuresis.Particularly river
Soviet Union, Hubei part personage among the people treat diabetes and have received significant curative effect with purslane secret recipe.
At present, drink is usually steeped with clear water decoction or boiling water with the dry product of purslane so that purslane natural component is distributed
In decoction liquor or soak.It is the method that purslane natural component is extracted in traditional sense with clear water decoction or boiling water bubble drink,
This method objectively causes the loss of natural (property of medicine) composition of purslane, causes to waste, and be not easy to different consumption
Crowd carries and uses.
The content of the invention
It is an object of the invention to provide a kind of natural component extracting method of purslane, crushed by purslane,
The natural component of purslane obtained by the step of alcohol extracting of impulse ultrasound waves, secondary alcohol extracting, concentration, alcohol precipitation and concentrate drying, it is maximum
Natural (property of medicine) composition of purslane is remained in degree, the loss of natural (property of medicine) composition of purslane is reduced, and should
The natural component of purslane has the function of improving body immunity, can be effectively used for treating the low symptom of body immunity,
Mitigate the pain of body immunity immunocompromised patients.
According to an aspect of the invention, there is provided the natural component extracting method of purslane, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed 2-3 times with clear water first, drained away the water and dry, after drying
Purslane be ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
6~8 times of ethanol solutions of amount of purslane meal are added, ultrasonic 1~2h of alcohol extracting is then carried out, first time extract solution is leached；
Step (3) secondary alcohol extracting：4~6 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath,
Then ultrasonic 2~2.5h of alcohol extracting is carried out, second extract solution is leached；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 70-80 DEG C in Rotary Evaporators, be 1.25-1.30 to solution relative density, obtained
Concentrate；
Step (5) alcohol precipitation：4~6 times of amount ethanol are added to refrigerate 10~12h, mistake at a temperature of putting -4-0 DEG C in concentrate
Filter, removes sediment, obtains supernatant；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation, concentrate is obtained, gained is dense
Contracting liquid is vacuum dried and crushes, and crushes the natural component that gained powder is purslane.
Further, the weight percent concentration of ethanol is 70~80% in step (2).
Further, the temperature for ultrasonic alcohol extracting being carried out in step (2) is：50-60℃.
Further, the weight percent concentration of ethanol is 55~65% in step (3).
Further, the temperature for ultrasonic alcohol extracting being carried out in step (3) is：65-75℃.
Further, the weight percent concentration of ethanol is 80~85% in step (5).
Further, step (4) concentration：Obtained by first time extract solution and step (3) obtained by combining step (2)
Secondary raffinate, is concentrated under reduced pressure in Rotary Evaporators at a temperature of 73-78 DEG C, is 1.26- to solution relative density
1.29, obtain concentrate.
Further, step (4) concentration：Obtained by first time extract solution and step (3) obtained by combining step (2)
Secondary raffinate, is concentrated under reduced pressure in Rotary Evaporators at a temperature of 75 DEG C, is 1.28 to solution relative density, obtains dense
Contracting liquid.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, 3- is taken every time
5g baths are diluted, and warmly take within 30 minutes after meal.
Beneficial effect：Crushed by purslane, the alcohol extracting of impulse ultrasound waves, secondary alcohol extracting, concentration, alcohol precipitation and dense
The natural component of the step gained purslane for contracting dry, remains natural (property of medicine) composition of purslane to the full extent, reduces
The loss of natural (property of medicine) composition of purslane, and the natural component of the purslane has the work(for improving body immunity
Can, can be effectively used for treating the low symptom of body immunity, mitigate the pain of body immunity immunocompromised patients.And due to this
The natural component of purslane is powdered, is easy to different consumer groups to carry and use.
Specific embodiment
Embodiment 1
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 3 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
6 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1h is then carried out, first time extract solution is leached.Wherein ethanol
Weight percent concentration is 75%, and the temperature of ultrasonic alcohol extracting is：55℃；
Step (3) secondary alcohol extracting：5 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath, so
After carry out ultrasonic alcohol extracting 2.3h, leach second extract solution.Wherein the weight percent concentration of ethanol is 60%, ultrasonic alcohol extracting
Temperature is：70℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 75 DEG C in Rotary Evaporators, be 1.28 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 4~6 times of amount ethanol to refrigerate 11h at a temperature of putting -3 DEG C in concentrate, filter, remove
Sediment, obtains supernatant.Wherein the weight percent concentration of ethanol is 83%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 96 DEG C), obtains dense
Contracting liquid, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, 4g is taken every time
Bath is diluted, and warmly takes within 30 minutes after meal.
Embodiment 2
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 2 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
6 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1h is then carried out, first time extract solution is leached.Wherein ethanol
Weight percent concentration is 70%, and the temperature of ultrasonic alcohol extracting is：50℃；
Step (3) secondary alcohol extracting：4 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath, so
After carry out ultrasonic alcohol extracting 2h, leach second extract solution.Wherein the weight percent concentration of ethanol is 55%, the temperature of ultrasonic alcohol extracting
Spend and be：65℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 70 DEG C in Rotary Evaporators, be 1.25 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 4 times of amount ethanol to refrigerate 10h at a temperature of putting -4 DEG C in concentrate, filter, it is heavy to remove
Starch, obtains supernatant.Wherein the weight percent concentration of ethanol is 80%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 96.5 DEG C), is obtained
Concentrate, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, 3g is taken every time
Bath is diluted, and warmly takes within 30 minutes after meal.
Embodiment 3
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 3 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
6.5 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1.2h is then carried out, first time extract solution is leached.Wherein second
The weight percent concentration of alcohol is 72%, and the temperature of ultrasonic alcohol extracting is：52℃；
Step (3) secondary alcohol extracting：4.5 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath,
Then ultrasonic alcohol extracting 2.1h is carried out, second extract solution is leached.Wherein the weight percent concentration of ethanol is 57%, ultrasonic alcohol extracting
Temperature be：67℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 72 DEG C in Rotary Evaporators, be 1.26 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 4.2 times of amount ethanol to refrigerate 10.2h at a temperature of putting -3 DEG C in concentrate, filter, remove
Sediment is removed, supernatant is obtained.Wherein the weight percent concentration of ethanol is 81%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 97 DEG C), obtains dense
Contracting liquid, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, take every time
3.3g baths are diluted, and warmly take within 30 minutes after meal.
Embodiment 4
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 2 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
6.7 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1.4h is then carried out, first time extract solution is leached.Wherein second
The weight percent concentration of alcohol is 74%, and the temperature of ultrasonic alcohol extracting is：54℃；
Step (3) secondary alcohol extracting：4.8 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath,
Then ultrasonic alcohol extracting 2.2h is carried out, second extract solution is leached.Wherein the weight percent concentration of ethanol is 59%, ultrasonic alcohol extracting
Temperature be：69℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 74 DEG C in Rotary Evaporators, be 1.27 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 4.5 times of amount ethanol to refrigerate 10.8h at a temperature of putting -2 DEG C in concentrate, filter, remove
Sediment is removed, supernatant is obtained.Wherein the weight percent concentration of ethanol is 82%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 98 DEG C), obtains dense
Contracting liquid, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, take every time
3.7g baths are diluted, and warmly take within 30 minutes after meal.
Embodiment 5
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 3 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
7 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1.6h is then carried out, first time extract solution is leached.Wherein ethanol
Weight percent concentration be 76%, the temperature of ultrasonic alcohol extracting is：56℃；
Step (3) secondary alcohol extracting：5.2 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath,
Then ultrasonic alcohol extracting 2.3h is carried out, second extract solution is leached.Wherein the weight percent concentration of ethanol is 61%, ultrasonic alcohol extracting
Temperature be：71℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 76 DEG C in Rotary Evaporators, be 1.28 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 5 times of amount ethanol to refrigerate 11h at a temperature of putting -2 DEG C in concentrate, filter, it is heavy to remove
Starch, obtains supernatant.Wherein the weight percent concentration of ethanol is 83%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 99 DEG C), obtains dense
Contracting liquid, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, 4g is taken every time
Bath is diluted, and warmly takes within 30 minutes after meal.
Embodiment 6
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 2 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
7.5 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 1.8h is then carried out, first time extract solution is leached.Wherein second
The weight percent concentration of alcohol is 78%, and the temperature of ultrasonic alcohol extracting is：58℃；
Step (3) secondary alcohol extracting：5.5 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath,
Then ultrasonic alcohol extracting 2.4h is carried out, second extract solution is leached.Wherein the weight percent concentration of ethanol is 63%, ultrasonic alcohol extracting
Temperature be：73℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 78 DEG C in Rotary Evaporators, be 1.29 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add 5.5 times of amount ethanol to refrigerate 11.5h at a temperature of putting -1 DEG C in concentrate, filter, remove
Sediment is removed, supernatant is obtained.Wherein the weight percent concentration of ethanol is 84%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 99.5 DEG C), is obtained
Concentrate, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, take every time
4.5g baths are diluted, and warmly take within 30 minutes after meal.
Embodiment 7
The natural component extracting method of purslane in the present embodiment, concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed with clear water 3 times first, drained away the water and dry, after drying
Purslane is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and
8 times of ethanol solutions of amount of purslane meal are added, ultrasonic alcohol extracting 2h is then carried out, first time extract solution is leached.Wherein ethanol
Weight percent concentration is 80%, and the temperature of ultrasonic alcohol extracting is：60℃；
Step (3) secondary alcohol extracting：6 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath, so
After carry out ultrasonic alcohol extracting 2.5h, leach second extract solution.Wherein the weight percent concentration of ethanol is 65%, ultrasonic alcohol extracting
Temperature is：75℃；
Step (4) is concentrated：Carrying for the second time obtained by the first time extract solution and step (3) obtained by combining step (2)
Liquid is taken, is concentrated under reduced pressure at a temperature of 80 DEG C in Rotary Evaporators, be 1.30 to solution relative density, obtain concentrate；
Step (5) alcohol precipitation：Add in the concentrate 6 times of amount ethanol to refrigerate 12h at a temperature of setting to 0 DEG C, filter, remove precipitation
Thing, obtains supernatant.Wherein the weight percent concentration of ethanol is 85%；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation (temperature is 99.1 DEG C), is obtained
Concentrate, gained concentrate is vacuum dried and crushed, and crushes the natural component that gained powder is purslane.
The natural component extracting method of purslane of the invention is oral drugs, instructions of taking：Once a day, 5g is taken every time
Bath is diluted, and warmly takes within 30 minutes after meal.
Beneficial effect：In the present invention each embodiment crushed by purslane, it is the alcohol extracting of impulse ultrasound waves, secondary
The step of alcohol extracting, concentration, alcohol precipitation and concentrate drying gained purslane natural component, the natural component of the purslane has and carries
The function of body immunity high, can be effectively used for treating the low symptom of body immunity, mitigate body immunity immunocompromised patients
Pain.And because the natural component of the purslane is powdered, it is easy to different consumer groups to carry and use.
The clinical observation on the therapeutic effect experiment of the oral drugs in the present invention obtained by the natural component extracting method of purslane：
First, physical data：400 patients meet the diagnostic criteria of hypoimmunity；Clinic is mainly shown as：Often sense
Emit, breathes hard is listless, light winter in summer weight, runny nose of gently then having a stuffy nose, headache is uncomfortable, heavy then aversion to cold afraid of the wind, sweating, cough etc..
Patient is randomly divided into 4 groups, every group 100；4 groups in age, sex, the course of disease and no significant difference on coincident with severity degree of condition.
2nd, treatment method
400 patients are randomly divided into 4 groups respectively, i.e.,：1 group is tested, 2 groups is tested, is tested 3 groups and 4 groups of experiment, every group each
100, the oral drug therapy obtained by 1 group of natural component extracting method using purslane in embodiment 3 of experiment, experiment 2
Group uses the oral drug therapy obtained by the natural component extracting method of purslane in embodiment 1；Test 3 groups and 4 groups of experiment
Using common drug treatment.
3rd, efficacy assessment standard
It is effective：Frequent flu, breathes hard is listless, and summer light winter weight, runny nose of gently then having a stuffy nose, headache is uncomfortable, heavy then be afraid of
The symptoms such as wind aversion to cold, sweating, cough disappear substantially.
Effectively：Frequent flu, breathes hard is listless, and summer light winter weight, runny nose of gently then having a stuffy nose, headache is uncomfortable, heavy then be afraid of
The symptoms such as wind aversion to cold, sweating, cough have clear improvement.
It is invalid：Frequent flu, breathes hard is listless, and summer light winter weight, runny nose of gently then having a stuffy nose, headache is uncomfortable, heavy then be afraid of
The symptoms such as wind aversion to cold, sweating, cough do not improve.
4th, clinical observation result
Clinical observation result is shown in Table 1.
The clinical observation on the therapeutic effect result table of table 1
| Number of cases | It is effective | Effectively | It is invalid | Total effective rate | |
| Test 1 group | 100 | 83 | 16 | 1 | 99% |
| Test 2 groups | 100 | 86 | 11 | 3 | 97% |
| Test 3 groups | 100 | 1 | 2 | 97 | 3% |
| Test 4 groups | 100 | 0 | 2 | 98 | 2% |
Clinical observation on the therapeutic effect result shows, the oral drugs pair obtained by the natural component extracting method of purslane of the present invention
There is significant curative effect in the patient of hypoimmunity；Test 1 group and 2 groups of experiment controls curative effect compared to 4 groups of 3 groups of experiment and experiment
Really, the gross efficiency of the oral drugs obtained by the natural component extracting method of purslane of the present invention has at least been higher by 94 percentages
The average total effective rate of 2 groups of point, 1 group of experiment and experiment is up to 98%；Result of the test explanation, the natural component extraction side of purslane
Oral drugs obtained by method can very significantly strengthen the immunity of patient.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, not
On the premise of departing from the invention design, various modifications and improvements can be made, these belong to protection model of the invention
Enclose.
Claims (8)
1. the natural component extracting method of purslane, it is characterised in that concrete technology step is as follows：
Step (1) is crushed：Fresh purslane is taken, is washed 2-3 times with clear water first, drained away the water and dry, by the horse after drying
Bitterroot is ground into meal；
Step (2) impulse ultrasound waves alcohol extracting：Purslane meal after crushing is put into pulse ultrasonic wave rinse bath, and is added
6~8 times of ethanol solutions of amount of purslane meal, then carry out ultrasonic 1~2h of alcohol extracting, leach first time extract solution；
Step (3) secondary alcohol extracting：4~6 times of ethanol solutions of amount of purslane meal are added in pulse ultrasonic wave rinse bath, then
Ultrasonic 2~2.5h of alcohol extracting is carried out, second extract solution is leached；
Step (4) is concentrated：Second extract solution obtained by first time extract solution and step (3) obtained by combining step (2),
It is concentrated under reduced pressure at a temperature of 70-80 DEG C in Rotary Evaporators, is 1.25-1.30 to solution relative density, is concentrated
Liquid；
Step (5) alcohol precipitation：4~6 times of amount ethanol are added to refrigerate 10~12h at a temperature of putting -4-0 DEG C in concentrate, filtering is removed
Sediment is removed, supernatant is obtained；
Step (6) concentrate drying：Supernatant obtained by step (5) is carried out into high temperature evaporation, concentrate is obtained, by gained concentrate
It is vacuum dried and crushes, crushes the natural component that gained powder is purslane.
2. the natural component extracting method of purslane according to claim 1, it is characterised in that ethanol is molten in step (2)
Liquid is weight percentage the aqueous solution of ethanol that concentration is 70~80%.
3. the natural component extracting method of purslane according to claim 1, it is characterised in that step is surpassed in (2)
The temperature of sound alcohol extracting is：50-60℃.
4. the natural component extracting method of purslane according to claim 1, it is characterised in that ethanol is molten in step (3)
Liquid is weight percentage the aqueous solution of ethanol that concentration is 55~65%.
5. the natural component extracting method of purslane according to claim 1, it is characterised in that step is surpassed in (3)
The temperature of sound alcohol extracting is：65-75℃.
6. the natural component extracting method of purslane according to claim 1, it is characterised in that ethanol is molten in step (5)
Liquid is weight percentage the aqueous solution of ethanol that concentration is 80~85%.
7. the natural component extracting method of purslane according to claim 1, it is characterised in that step (4) is concentrated：Merge
Second extract solution obtained by first time extract solution and step (3) obtained by step (2), the 73-78 in Rotary Evaporators
It is concentrated under reduced pressure at a temperature of DEG C, is 1.26-1.29 to solution relative density, is obtained concentrate.
8. the natural component extracting method of purslane according to claim 7, it is characterised in that step (4) is concentrated：Merge
Second extract solution obtained by first time extract solution and step (3) obtained by step (2), 75 DEG C in Rotary Evaporators
At a temperature of be concentrated under reduced pressure, to solution relative density be 1.28, obtain concentrate.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107890542A (en) * | 2017-12-26 | 2018-04-10 | 湖南知达医药科技有限公司 | Treat the Chinese medicine composition and its preparation method of diarrhoea |
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