CN112516269A - Plant extract composition for resisting vibriosis and preparation method and application thereof - Google Patents
Plant extract composition for resisting vibriosis and preparation method and application thereof Download PDFInfo
- Publication number
- CN112516269A CN112516269A CN202011524684.7A CN202011524684A CN112516269A CN 112516269 A CN112516269 A CN 112516269A CN 202011524684 A CN202011524684 A CN 202011524684A CN 112516269 A CN112516269 A CN 112516269A
- Authority
- CN
- China
- Prior art keywords
- leaf extract
- parts
- stem
- extract
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9062—Alpinia, e.g. red ginger or galangal
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Insects & Arthropods (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Birds (AREA)
- Marine Sciences & Fisheries (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Fodder In General (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of aquaculture, and particularly relates to a plant extract composition for resisting vibriosis and a preparation method and application thereof. The plant extract composition comprises, by weight, 20-60 parts of alpinia oxyphylla stem and leaf extract, 20-50 parts of galangal stem and leaf extract, 10-30 parts of olive leaf extract and 10-30 parts of mango leaf extract. The research of the invention finds that the plant extract composition added into the feed can obviously improve the survival rate of the litopenaeus vannamei, promote the growth, enhance the activity and the oxidation resistance of the immunoenzyme in the hepatopancreas and improve the survival rate after vibrio infection; the raw materials in the invention are byproducts of economic crops and Chinese herbal medicine planting, and have the advantages of low price, easy obtainment, simple process and environmental protection.
Description
Technical Field
The invention relates to the field of aquaculture, and particularly relates to a plant extract composition for resisting vibriosis and a preparation method and application thereof.
Background
The types of shrimps cultured in China are more, and the shrimps mainly comprise litopenaeus vannamei, penaeus monodon, penaeus japonicus, procambarus clarkia, red swamp crayfish, macrobrachium rosenbergii, macrobrachium nipponensis and the like. China is the main breeding country of shrimps and the largest consumer country, and a large amount of shrimps need to be imported from Vietnam, Thailand, Ecuador and other countries every year to meet the increasing demands of domestic people.
In recent years, the domestic shrimp culture industry has the problems of easy morbidity of shrimp seedlings, low culture success rate and lack of efficient functional compound feed. In 13 years from 2003 to 2015, the domestic litopenaeus vannamei breeding yield is improved from 61 million tons to 162 million tons, but the domestic breeding success rate as low as 10% -20% is not negligible, and compared with the quality of imported shrimps, the domestic bred shrimps have the disadvantages of small commercial shrimps, high proportion of diseased shrimps, poor flavor and the like, and the reasons for the disadvantages are independent of breeding modes and increasingly deteriorated breeding environments.
In recent years, domestic environmental protection is continuously changed and upgraded. The aquaculture industry which is concerned with the national civilization is not exceptional, the illegal and laggard productivity is banned on a large scale, and the illegal administration of antibiotics and medicaments which are toxic and harmful to human bodies is prohibited. Ultimately, this will shuffle the existing feed industry from new, as the farming structure adjusts. The feed is in line with the market demand, and the compound feed added with the plant extract for promoting the growth performance of the litopenaeus vannamei and improving the disease-resistant immunity becomes the direction of the aquatic product industry.
The plant contains a plurality of natural safe antibacterial and anti-inflammatory components, and is a main resource for developing an aquatic animal anti-bacterial product. In the traditional utilization process of some economic crops and Chinese herbal medicines, parts such as stems, leaves and the like are often abandoned and cannot be utilized, but the active ingredients of the parts are not inferior to those of medicinal parts and edible parts, so that the development of Chinese herbal medicine extracts which can improve the immunity and disease resistance of shrimps and replace antibiotics by utilizing the plant wastes becomes a hot point of research, and meanwhile, the Chinese herbal medicine extracts which are easy to obtain raw materials and low in price are obtained.
Disclosure of Invention
In view of the above, the present invention provides a plant extract composition for resisting vibriosis, a preparation method thereof and applications thereof. The research shows that the survival rate of the litopenaeus vannamei boone can be improved, the growth performance can be improved, and the immunity can be enhanced by adding the plant extract composition which is composed of the alpinia oxyphylla stem and leaf extract, the galangal stem and leaf extract, the olive leaf extract and the mango leaf extract and has proper concentration. Thereby being applied to the growth of litopenaeus vannamei and other aquatic animals and the prevention and treatment of vibrio diseases.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an anti-vibriosis plant extract composition, which comprises, by weight, 20-60 parts of alpinia oxyphylla stem and leaf extract, 20-50 parts of galangal stem and leaf extract, 10-30 parts of olive leaf extract and 10-30 parts of mango leaf extract.
Preferably, the plant extract composition comprises 30-60 parts by weight of alpinia oxyphylla stem and leaf extract, 20-50 parts by weight of galangal stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
Preferably, the plant extract composition comprises 30 parts by weight of alpinia oxyphylla stem and leaf extract, 50 parts by weight of galangal stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
Preferably, the plant extract composition comprises 60 parts by weight of alpinia oxyphylla stem and leaf extract, 20 parts by weight of galangal stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
The invention also provides application of the plant extract composition in preparing a medicament or feed for resisting vibriosis.
Preferably, the vibriosis is a shrimp vibriosis.
In the specific embodiment provided by the invention, the vibriosis is vibriosis of litopenaeus vannamei.
Preferably, the vibriosis is one or more of vibriosis alginolyticus, vibriosis parahaemolyticus, vibriosis anguillarum and vibriosis vulnificus.
The invention also provides a preparation method of the plant extract composition, which comprises the following steps:
preparing the alpinia oxyphylla stem and leaf extract and the galangal stem and leaf extract: taking plant stems and leaves, adding ethanol water solution with the mass concentration of 75% -95% according to the material-liquid ratio of 1 (10-20) in terms of g/L, leaching for 2-4 times at the temperature of 15-35 ℃, each time for 6-8 days, filtering the obtained leaching solution, concentrating under reduced pressure, and drying;
preparing olive leaf extract and mango leaf extract: taking plant leaves, adding ethanol water solution with the mass concentration of 75% -95% according to the material-liquid ratio of 1 (5-10) in terms of g/L, leaching for 2-4 times at 15-35 ℃, each time for 4-6 days, centrifuging the obtained leaching solution, taking supernatant, concentrating under reduced pressure, and drying;
mixing the stem and leaf extract of Alpinia oxyphylla, the stem and leaf extract of Alpinia galanga, the olive leaf extract and the mango leaf extract to obtain the plant extract composition.
In the specific embodiment provided by the invention, the preparation of the alpinia oxyphylla stem and leaf extract and the galangal stem and leaf extract comprises the following steps: adding ethanol water solution with mass concentration of 95% into plant stem and leaf according to the feed-liquid ratio of 1:10 in g/L, leaching for 3 times at room temperature for 6 days each time, filtering the obtained leaching solution, concentrating under reduced pressure, and drying.
In the specific embodiment provided by the invention, the preparation of the olive leaf extract and the mango leaf extract comprises the following steps: taking plant leaves, adding ethanol water solution with the mass concentration of 95% according to the feed-liquid ratio of 1:5 in terms of g/L, leaching for 3 times at room temperature for 4 days each time, centrifuging the obtained leaching solution, taking supernatant, concentrating under reduced pressure, and drying.
In the present invention, the plant stems and leaves are dried plant stems and leaves or fresh plant stems and leaves.
In the present invention, the plant leaves are dried plant leaves or fresh plant leaves.
The invention also provides a compound feed, which comprises a basic feed and the plant extract composition.
In the present invention, the feed may be a compound feed or a pellet feed having a plant extract composition attached to the surface thereof.
Preferably, when the feed is a compound feed, the addition amount of the plant extract composition is 0.1-0.5 g/kg of basal feed;
preferably, when the feed is a pellet feed with the plant extract composition attached to the surface, the addition amount of the plant extract composition is 0.3-0.8 g/kg.
Preferably, the addition amount of the plant extract composition is 0.4-0.5 g/kg.
Preparing a compound feed: dispersing the above extracts in water to obtain suspension, adding into the above materials, stirring, granulating, and oven drying.
Preparing the pellet feed: dispersing the extracts in water to form turbid liquid, uniformly spraying the turbid liquid on the surface of the feed, and air-drying the turbid liquid at normal temperature for 1-2 hours.
The invention provides a plant extract composition for resisting vibriosis and a preparation method and application thereof. The plant extract composition comprises, by weight, 20-60 parts of alpinia oxyphylla stem and leaf extract, 20-50 parts of galangal stem and leaf extract, 10-30 parts of olive leaf extract and 10-30 parts of mango leaf extract. The invention has the following technical effects:
the research of the invention finds that the plant extract composition added into the feed can obviously improve the survival rate of the litopenaeus vannamei, promote the growth, enhance the activity and the oxidation resistance of the immunoenzyme in the hepatopancreas and improve the survival rate after vibrio infection;
the stem and leaf of Alpinia galanga will contain flavonoid, volatile oil, diphenyl heptane compounds, curcumin, etc., and has antibacterial, antioxidant, and free radical scavenging effects; the olive leaves are rich in oleuropein, and have broad-spectrum antibacterial activity and antioxidant activity; the mango leaves mainly contain a plurality of chemical components such as ascorbic acid, mangiferin, shikimin, quercetin, kaempferol and the like, and have the functions of immunoregulation, anti-inflammation, antibiosis, antivirus and the like. The alpinia oxyphylla stem and leaf extract, the galangal stem and leaf extract, the olive leaf extract and the mango leaf extract in the composition can generate obvious synergistic effect;
the raw materials in the invention are byproducts of economic crops and Chinese herbal medicine planting, and have the advantages of low price, easy obtainment, simple process and environmental protection.
Detailed Description
The invention discloses a plant extract composition for resisting vibriosis and a preparation method and application thereof, and can be realized by appropriately improving process parameters by taking the contents as reference by the technical personnel in the field. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The plant extract composition for resisting vibriosis, the preparation method and the raw materials, reagents or instruments used in the application of the plant extract composition can be purchased from the market.
The invention is further illustrated by the following examples:
example 1
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 20 parts of galangal stem and leaf extract, 30 parts of olive leaf extract and 20 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.3g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Example 2
The plant extract composition comprises the following components in parts by weight: 60 parts of alpinia oxyphylla stem and leaf extract, 20 parts of galangal stem and leaf extract, 10 parts of olive leaf extract and 10 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.4g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Example 3
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 50 parts of galangal stem and leaf extract, 10 parts of olive leaf extract and 10 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.5g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Example 4
The plant extract composition comprises the following components in parts by weight: 20 parts of alpinia oxyphylla stem and leaf extract, 20 parts of galangal stem and leaf extract, 30 parts of olive leaf extract and 30 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.6g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Example 5
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 30 parts of galangal stem and leaf extract, 20 parts of olive leaf extract and 20 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.8g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Comparative example 1
Commercial compound feed for prawns without addition of plant extract composition.
Comparative example 2
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 50 parts of galangal stem and leaf extract and 10 parts of olive leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
The olive leaves were extracted as a crude extract by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.5g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Comparative example 3
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 50 parts of galangal stem and leaf extract and 10 parts of mango leaf extract.
Extracting crude extracts of alpinia oxyphylla stems and leaves and galangal stems and leaves by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting a crude extract from mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.5g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Comparative example 4
The plant extract composition comprises the following components in parts by weight: 30 parts of alpinia oxyphylla stem and leaf extract, 10 parts of olive leaf extract and 10 parts of mango leaf extract.
Extracting crude extracts of the stems and leaves of the Alpinia oxyphylla by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.5g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Comparative example 5
The plant extract composition comprises the following components in parts by weight: 50 parts of galangal stem and leaf extract, 10 parts of olive leaf extract and 10 parts of mango leaf extract.
Extracting crude extracts of the stems and leaves of galangal by the following method: cleaning plant stems and leaves, chopping, drying, adding 95% ethanol according to a material-liquid ratio of 1:10(g/L), leaching at room temperature for 3 times, 6 days each time, filtering the obtained leaching solution with gauze, concentrating under reduced pressure, and drying to obtain extract.
Extracting crude extracts of olive leaves and mango leaves by the following method: cleaning plant leaves, oven drying, pulverizing, adding 95% ethanol at a ratio of 1:5(g/L), extracting at room temperature for 4 days for 3 times, centrifuging the obtained extractive solution at 8000r/min for 5min, collecting supernatant, concentrating under reduced pressure, and drying to obtain extract.
The addition amount of the composition is 0.5g/kg, the crude extracts are taken according to the parts by weight, dispersed in water to form suspension, evenly sprayed on the surface of commercial compound feed for prawns, and air-dried for 2 hours at normal temperature.
Experimental example 1 breeding test:
the feeds of examples 1 to 5 and comparative examples 1 to 5 were air-dried in the shade for 2 hours and transferred to a refrigerator at-20 ℃ for further use.
The Litopenaeus vannamei is bred in a breeding box, the specification of the breeding box is that the length, the width and the height are 50 x 40cm, the water depth is 30cm, the water quality is kept stable in the whole breeding period, the water temperature is controlled to be 28-33 ℃, the dissolved oxygen is more than 6.0mg/L, the pH is 7.2-7.5, and the salinity is 16-18 per mill. Each group had three replicates, and 30 replicates each were stocked. The duration of the whole experiment is 8 weeks, the feeding is carried out once every day at 7:00 am, 11:00 am, 15:00 pm and 19:00 pm, residual baits are collected by using a siphon tube 40min after each feeding, the residual baits are dried in an oven at 70 ℃, and the feeding amount and the residual baits are recorded. The water is changed every day, and the water changing amount is about 1/3 of the whole culture water tank. After the test is finished, the growth performance of the litopenaeus vannamei is determined and shown in table 1, hemolymph and hepatopancreas of each group are taken, the activity of the immunoenzyme is determined, and the result is shown in table 2.
Vibrio challenge experiment: taking each group of Litopenaeus vannamei Boone which is fed with the plant extract composition for 8 weeks, wherein each group comprises three parallel groups, and each group comprises 10 parallel groups of Litopenaeus vannamei Boone. The water temperature is controlled at 28-33 ℃, the dissolved oxygen is above 6.0mg/L, and the pH is 7.2-7.5. And respectively injecting bacterial solutions of vibrio alginolyticus and vibrio parahaemolyticus in prawn abdominal nodes, and counting the mortality rate for 48 hours. The results are shown in Table 3.
TABLE 1 Effect of the addition of plant extract compositions on the growth Performance of Litopenaeus vannamei
| Group of | Weight gain (g) | Survival rate (%) | Coefficient of bait | Fullness of fertilizer |
| Comparative example 1 | 718.69±17.73 | 80.00±3.33 | 1.51±0.06 | 4.89±0.15 |
| Comparative example 2 | 733.95±38.27 | 73.33±6.67 | 1.48±0.09 | 4.92±0.12 |
| Comparative example 3 | 728.06±51.42 | 83.33±3.33 | 1.44±0.11 | 4.88±0.09 |
| Comparative example 4 | 759.61±30.88 | 81.11±6.94 | 1.41±0.07 | 5.09±0.12 |
| Comparative example 5 | 738.79±62.78 | 82.22±3.85 | 1.45±0.12 | 5.11±0.13 |
| Example 1 | 791.22±40.94 | 91.11±5.09 | 1.36±0.08 | 5.36±0.09 |
| Example 2 | 821.83±25.65 | 92.22±3.85 | 1.31±0.06 | 5.62±0.08 |
| Example 3 | 838.66±76.65 | 93.33±3.33 | 1.30±0.13 | 5.53±0.07 |
| Example 4 | 853.99±41.24 | 91.11±1.92 | 1.31±0.10 | 5.68±0.09 |
| Example 5 | 796.49±39.54 | 88.89±1.92 | 1.34±0.13 | 5.62±0.10 |
TABLE 2 Effect of the addition of the plant extract composition on the Immunity of Litopenaeus vannamei
TABLE 3 Effect of the addition of the plant extract composition on Vibrio of Litopenaeus vannamei (%)
| Group of | Vibrio alginolyticus 48h mortality rate | 48h mortality rate of toxic attack of vibrio parahaemolyticus |
| Comparative example 1 | 76.7±5.8 | 80±10 |
| Comparative example 2 | 73.3±5.8 | 73.3±5.8 |
| Comparative example 3 | 70.0±10.0 | 73.3±5.8 |
| Comparative example 4 | 70.0±10.0 | 70.0±0.0 |
| Comparative example 5 | 63.3±5.8 | 70.0±10.0 |
| Example 1 | 50.0±10.0 | 53.3±5.8 |
| Example 2 | 46.7±5.8 | 40.0±10.0 |
| Example 3 | 40.0±10.0 | 46.7±5.8 |
| Example 4 | 40.0±10.0 | 56.7±5.8 |
| Example 5 | 43.3±15.3 | 46.7±11.5 |
As can be seen from table 1, the survival rate of litopenaeus vannamei of examples 1 to 5 was significantly higher than that of the comparative example group. On the aspect of growth performance, each implementation group is superior to the comparison group in the aspects of weight gain rate and bait coefficient, bait consumption is effectively reduced, and on the other hand, the fertility of the litopenaeus vannamei of the implementation group is increased, which indicates that the edible part of the litopenaeus vannamei is increased. The effect in the group of example 3 is the best, and the difference is obvious from the comparative group, which is probably because the galangal stem and leaf extract has the efficacy of protecting liver and gallbladder, the survival rate of the culture is improved, and the metabolic function of the litopenaeus vannamei is improved.
As can be seen from Table 2, the immunoenzyme activity and antioxidant capacity of the litopenaeus vannamei liver pancreas of the groups of examples 1-5 are superior to those of the comparative example, indicating that the immune function of the body of the litopenaeus vannamei of the examples is improved.
As can be seen from Table 3, the mortality of the litopenaeus vannamei of the groups of examples 1 to 5 is significantly reduced compared with the control group, which indicates that the addition of the plant extract composition to the feed for the litopenaeus vannamei can significantly improve the capability of the litopenaeus vannamei against vibrio toxicity.
The invention adopts plant stems and leaves which are non-medicine parts of medicinal plants or leaves of fruit trees and are agricultural wastes, so that the agricultural wastes can be fully utilized and the income of crops is increased; on the other hand, the raw materials of the invention are pure natural, have no chemical toxic residue, and the raw materials are easy to obtain, the cost is low, and the process is simple.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A plant extraction composition for resisting vibriosis is characterized by comprising, by weight, 20-60 parts of alpinia oxyphylla stem and leaf extract, 20-50 parts of galangal stem and leaf extract, 10-30 parts of olive leaf extract and 10-30 parts of mango leaf extract.
2. The plant extract composition as claimed in claim 1, which comprises 30-60 parts by weight of Alpinia oxyphylla stem and leaf extract, 20-50 parts by weight of Alpinia galanga stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
3. The plant extract composition as claimed in claim 1, which comprises 30 parts by weight of Alpinia oxyphylla stem and leaf extract, 50 parts by weight of Alpinia galanga stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
4. The plant extract composition as claimed in any one of claims 1 to 3, which comprises 60 parts by weight of Alpinia oxyphylla stem and leaf extract, 20 parts by weight of Alpinia galanga stem and leaf extract, 10 parts by weight of olive leaf extract and 10 parts by weight of mango leaf extract.
5. Use of a plant extract composition according to any one of claims 1 to 4 in the manufacture of a medicament or feed for combating vibriosis.
6. Use according to claim 5, wherein the vibriosis is of shrimp species.
7. The use of claim 5 or 6, wherein the vibriosis is one or more of vibrio alginolyticus, vibrio parahaemolyticus, vibrio anguillarum and vibrio vulnificus.
8. A method of preparing a plant extract composition as claimed in any one of claims 1 to 4, comprising the steps of:
preparing the alpinia oxyphylla stem and leaf extract and the galangal stem and leaf extract: taking plant stems and leaves, adding ethanol water solution with the mass concentration of 75% -95% according to the material-liquid ratio of 1 (10-20) in terms of g/L, leaching for 2-4 times at the temperature of 15-35 ℃, each time for 6-8 days, filtering the obtained leaching solution, concentrating under reduced pressure, and drying;
preparing olive leaf extract and mango leaf extract: taking plant leaves, adding ethanol water solution with the mass concentration of 75% -95% according to the material-liquid ratio of 1 (5-10) in terms of g/L, leaching for 2-4 times at 15-35 ℃, each time for 4-6 days, centrifuging the obtained leaching solution, taking supernatant, concentrating under reduced pressure, and drying;
mixing the stem and leaf extract of Alpinia oxyphylla, the stem and leaf extract of Alpinia galanga, the olive leaf extract and the mango leaf extract to obtain the plant extract composition.
9. A feed comprising a basal feed and the plant extract composition of any one of claims 1 to 4; the feed is a compound feed or a pellet feed with the plant extract composition attached to the surface.
10. The compound feed according to claim 9, wherein when the feed is a compound feed, the amount of the plant extract composition added is 0.1 to 0.5g/kg of basal feed;
when the feed is a pellet feed with the plant extract composition attached to the surface, the addition amount of the plant extract composition is 0.3-0.8 g/kg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011524684.7A CN112516269B (en) | 2020-12-22 | 2020-12-22 | Plant extract composition for resisting vibriosis and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011524684.7A CN112516269B (en) | 2020-12-22 | 2020-12-22 | Plant extract composition for resisting vibriosis and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112516269A true CN112516269A (en) | 2021-03-19 |
| CN112516269B CN112516269B (en) | 2022-05-03 |
Family
ID=75002175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011524684.7A Active CN112516269B (en) | 2020-12-22 | 2020-12-22 | Plant extract composition for resisting vibriosis and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112516269B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113243460A (en) * | 2021-05-17 | 2021-08-13 | 海南大学 | Immunodeficiency mouse feed and preparation method thereof |
| CN114504064A (en) * | 2022-01-15 | 2022-05-17 | 中国热带农业科学院热带生物技术研究所 | Application of alpinia oxyphylla stem and leaf extract as litopenaeus vannamei feed additive |
| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
| CN117256731A (en) * | 2023-09-14 | 2023-12-22 | 中国热带农业科学院热带生物技术研究所 | Black soldier fly larva fermentation liquor, preparation method and application thereof, and shrimp feed |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496558A (en) * | 2009-03-17 | 2009-08-05 | 湛江市第一兽药厂 | Natural plant feed addictive for improving immunity of prawn |
| CN106924449A (en) * | 2017-03-30 | 2017-07-07 | 广州市普精生物科技有限公司 | One kind prevents and treats vibriosis Chinese medicine composition and its preparation method and application |
-
2020
- 2020-12-22 CN CN202011524684.7A patent/CN112516269B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496558A (en) * | 2009-03-17 | 2009-08-05 | 湛江市第一兽药厂 | Natural plant feed addictive for improving immunity of prawn |
| CN106924449A (en) * | 2017-03-30 | 2017-07-07 | 广州市普精生物科技有限公司 | One kind prevents and treats vibriosis Chinese medicine composition and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| 李钟美等: "高良姜提取物抑菌活性及稳定性研究", 《食品与机械》 * |
| 梁方方等: "中草药作为饲料添加剂防治弧菌的研究进展", 《饲料研究》 * |
| 管淑玉等: "5种中草药提取物对致病性弧菌的抑菌作用", 《广东药学院学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113243460A (en) * | 2021-05-17 | 2021-08-13 | 海南大学 | Immunodeficiency mouse feed and preparation method thereof |
| CN114504064A (en) * | 2022-01-15 | 2022-05-17 | 中国热带农业科学院热带生物技术研究所 | Application of alpinia oxyphylla stem and leaf extract as litopenaeus vannamei feed additive |
| CN114504064B (en) * | 2022-01-15 | 2023-10-13 | 中国热带农业科学院热带生物技术研究所 | Application of extract of stem and leaf of alpinia oxyphylla as feed additive for litopenaeus vannamei |
| CN115212276A (en) * | 2022-07-14 | 2022-10-21 | 集美大学 | External Chinese herbal medicine composition for preventing and treating shrimp and crab ciliate diseases |
| CN115212276B (en) * | 2022-07-14 | 2023-11-21 | 集美大学 | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab |
| CN117256731A (en) * | 2023-09-14 | 2023-12-22 | 中国热带农业科学院热带生物技术研究所 | Black soldier fly larva fermentation liquor, preparation method and application thereof, and shrimp feed |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112516269B (en) | 2022-05-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112516269B (en) | Plant extract composition for resisting vibriosis and preparation method and application thereof | |
| KR101623744B1 (en) | Feed composition for Protaetia brevitarsis seulensis | |
| EP2983496B1 (en) | Supplemented fish feed | |
| CN104273184A (en) | Insect-resistant pesticide for vegetables and preparation method of insect-resistant pesticide | |
| EP0600080A4 (en) | Organic pesticide. | |
| CN102626117A (en) | Natural green bio-organic pesticide composition and its application | |
| CN106135318A (en) | A kind of Chinese medicine composition and its preparation method and application | |
| CN104782550A (en) | Cultivation method of prawns | |
| CN104206787B (en) | A kind of poultry fodder additive and preparation method thereof | |
| CN104256172A (en) | Odontobutis obscura parent fish feed and preparation method thereof | |
| CN101720791A (en) | Matrine pure plant source pesticide | |
| JP3474198B2 (en) | Animal feed composition | |
| CN106665426A (en) | Method for preventing hemorragic disease of grass carp | |
| CN107361204A (en) | The preparation method and animal feed of a kind of honeysuckle extract feed additive | |
| KR101104326B1 (en) | Feed additive | |
| CN115212276B (en) | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab | |
| CN103609909B (en) | Pollution-free aquatic feed additive using ginkgo leaves as base stock | |
| CN104839486A (en) | Pelteobagrus fulvidraco feed additive with liver protection function and application thereof | |
| CN105851662A (en) | Traditional Chinese medicine residue additive of chicken feed and preparation method | |
| CN107624810A (en) | A kind of Pesticidal combination | |
| CN103283958B (en) | Formulation and preparation method of Chinese herbal feed additive for livestock and poultry | |
| CN108815426A (en) | A kind of antibacterial agent and its preparation method and application containing natural plant extract | |
| WO2019146827A1 (en) | Composition for preventing or treating parasitic infections in fish, comprising helianthus tuberosus extract and sophora flavescens aiton extract | |
| CN105724450B (en) | A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry | |
| JP2006061107A (en) | Parasitic disease inhibitor, feed for marine cultured fishes and method for preventing parasitic disease of marine cultured fishes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |