CN112514595A - Rhizosphere growth-promoting bacterium seed soaking method for improving germination rate - Google Patents
Rhizosphere growth-promoting bacterium seed soaking method for improving germination rate Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention relates to the technical field of rhizosphere growth-promoting bacteria seed soaking, and discloses a rhizosphere growth-promoting bacteria seed soaking method for improving germination rate, which comprises the following raw materials in parts by weight: 1-3 parts of enterobacter raw cancer, 1-3 parts of ferment viable bacteria, 3-6 parts of plant growth regulator, 5-10 parts of ammonium salt, 5-10 parts of potassium salt, 5-10 parts of plant ash, 3-5 parts of amino acid zinc, 5-10 parts of calcium chloride, 5-10 parts of carbendazim and 25-30 parts of water. According to the rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate, the division speed and the water absorption speed of embryo cells of the seeds can be greatly improved by adding the rhizosphere growth-promoting bacterium enterobacter carcinosus, the germination speed of the seeds is accelerated, the germination rate of the seeds is improved, the rhizosphere growth-promoting bacterium seed soaking liquid also contains nutrient components required by a plurality of seeds in the germination process, the requirement for improving the ingestion of required elements of the seeds in the germination process can be met, meanwhile, some worm eggs and viruses can be killed, the germination rate of the seeds is further improved, and the phenomenon that the seeds are killed due to the loss of activity of the worm eggs or the viruses is avoided.
Description
Technical Field
The invention relates to the technical field of rhizosphere growth-promoting bacteria seed soaking, in particular to a rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate.
Background
The germination rate refers to the percentage of the germination number of the tested seeds in the total number of the tested seeds. The germination rate is one of the important indexes for detecting the seed quality, the seed consumption is often calculated according to the germination rate in agricultural production, and the germination rate refers to the percentage of the germination number of the tested seeds in the total number of the tested seeds. If 95 test seeds germinate in 100 test seeds, the germination rate is 95%. The germination rate is one of the important indexes for detecting the seed quality, the seed consumption amount is usually calculated according to the seed consumption amount in agricultural production, the seed soaking rate can be greatly improved, the seed is a flow of agricultural planting, the seed is required to be soaked before sowing for the seed with slower germination, the seed soaking purpose is to promote the seed to germinate earlier and kill some ova and viruses, the seed soaking purpose is to ensure that the seeds and the grains absorb water more quickly to achieve the water content capable of normally germinating, the seed soaking requires the seed soaking, and the seed soaking liquid is usually a mixed liquid.
The existing seed soaking mixed liquor can only provide germination water and kill some worm eggs for the seeds singly, and the seed soaking mode cannot greatly improve the germination rate of the seeds.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate, has the advantages of supplementing germination nutrient elements to seeds and improving the germination rate, and solves the problem of low germination rate of the existing seed soaking.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate comprises the following raw materials in parts by weight: 1-3 parts of enterobacter carcinogenes, 1-3 parts of enzyme live bacteria, 3-6 parts of plant growth regulator, 5-10 parts of ammonium salt, 5-10 parts of potassium salt, 5-10 parts of plant ash, 3-5 parts of amino acid zinc, 5-10 parts of calcium chloride, 5-10 parts of carbendazim and 25-30 parts of water;
the rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate comprises the following steps:
s1 preparation of plant growth regulator
One or more raw materials of the brassinolide, the salicylic acid, the jasmonic acid, the paclobutrazol and the polyamine are selected through a mixing container, mixed to prepare the plant growth regulator, and the required amount is weighed for backup.
S2, ammonium and potassium salt preparation
Selecting one or two of ammonium carbonate and ammonium bicarbonate to mix by a mixing container to prepare the required ammonium salt, weighing the required ammonium salt for backup, selecting one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate to mix by the mixing container to prepare the required potassium salt, and weighing the required potassium salt for backup.
S3, weighing and backing up
The method comprises the steps of weighing the required proportion of the enterobacter carcinosus, the live ferment bacteria, the plant ash, the zinc amino acid, the calcium chloride, the carbendazim and the water, and carrying out backup.
S4, mixing the raw materials
The preparation method comprises the steps of putting the backup plant ash, the amino acid zinc, the calcium chloride and the carbendazim into a mixing device, mixing for 4-6 minutes, adding the backup water after mixing, heating, stirring and mixing, and naturally cooling after stirring to obtain a mixed solution.
S5, adding growth-promoting rhizobacteria
Adding the rhizosphere growth-promoting bacterium enterobacter tumefaciens and the ferment viable bacteria into the mixed solution in the mixing equipment, mixing, and finally obtaining the finished product of rhizosphere growth-promoting bacterium seed soaking solution.
S6, selecting seeds
And selecting seeds with full grains, no wormholes and no cracks.
S7 seed soaking
And (3) soaking the seeds in the seed soaking solution containing the rhizosphere growth-promoting bacteria, taking out the seeds after the seed soaking solution is finished, and drying or airing the seeds to finish the seed soaking process.
Preferably, the plant growth regulator is prepared by mixing one or more of brassinolide, salicylic acid, jasmonic acid, paclobutrazol and polyamine.
Preferably, the ammonium salt is one or two of ammonium carbonate and ammonium bicarbonate.
Preferably, the potassium salt is one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate.
Preferably, the temperature for heating, mixing and stirring in the step S4 is 30-40 ℃, and the stirring time is 5-10 minutes.
Preferably, the natural cooling time in the step S4 is 25 to 30 minutes.
Preferably, the seed soaking time of the step S6 is 2-3 hours.
Preferably, the drying or airing degree in the step S6 is that no liquid drops are on the surface of the seeds.
(III) advantageous effects
Compared with the prior art, the invention provides the rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate, and the method has the following beneficial effects:
the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate can greatly improve the division speed of embryo cells and the water absorption speed by adding the rhizosphere growth-promoting bacteria enterobacter carcinosus, accelerate the germination speed of seeds and improve the germination rate of the seeds, and the rhizosphere growth-promoting bacteria seed soaking liquid also contains nutrient components required by a plurality of seeds in the germination process, so that the requirement of improving the ingestion of required elements of the seeds in the germination process can be met, the germination rate of the seeds is improved, meanwhile, some insect eggs and viruses can be killed, the germination rate of the seeds is further improved, and the phenomenon that the seeds are killed due to the inactivation of the insect eggs or the viruses is avoided.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate comprises the following raw materials in parts by weight: 1 part of enterobacter carcinogenes, 1 part of ferment viable bacteria, 3 parts of plant growth regulator, 5 parts of ammonium salt, 5 parts of potassium salt, 5 parts of plant ash, 3 parts of amino acid zinc, 5 parts of calcium chloride, 5 parts of carbendazim and 25 parts of water.
The rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate comprises the following steps:
s1 preparation of plant growth regulator
One or more raw materials of the brassinolide, the salicylic acid, the jasmonic acid, the paclobutrazol and the polyamine are selected through a mixing container, mixed to prepare the plant growth regulator, and the required amount is weighed for backup.
S2, ammonium and potassium salt preparation
Selecting one or two of ammonium carbonate and ammonium bicarbonate to mix by a mixing container to prepare the required ammonium salt, weighing the required ammonium salt for backup, selecting one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate to mix by the mixing container to prepare the required potassium salt, and weighing the required potassium salt for backup.
S3, weighing and backing up
The method comprises the steps of weighing the required proportion of the enterobacter carcinosus, the live ferment bacteria, the plant ash, the zinc amino acid, the calcium chloride, the carbendazim and the water, and carrying out backup.
S4, mixing the raw materials
The preparation method comprises the steps of putting the backup plant ash, the amino acid zinc, the calcium chloride and the carbendazim into a mixing device, mixing for 4-6 minutes, adding backup water after mixing, heating, stirring and mixing for 5-10 minutes at the temperature of 30-40 ℃, naturally cooling after stirring, and naturally cooling for 25-30 minutes to obtain a mixed solution.
S5, adding growth-promoting rhizobacteria
Adding the rhizosphere growth-promoting bacterium enterobacter tumefaciens and the ferment viable bacteria into the mixed solution in the mixing equipment, mixing, and finally obtaining the finished product of rhizosphere growth-promoting bacterium seed soaking solution.
S6, selecting seeds
And selecting seeds with full grains, no wormholes and no cracks.
S7 seed soaking
Soaking the seeds in the seed soaking liquid containing the rhizosphere growth promoting bacteria for 2-3 hours, taking out the seeds after the seed soaking is finished, and drying or airing the seeds to the extent that no liquid drops exist on the surfaces of the seeds, thus completing the seed soaking process.
The plant growth regulator is prepared by mixing one or more of brassinolide, salicylic acid, jasmonic acid, paclobutrazol and polyamine.
The ammonium salt is one or two of ammonium carbonate and ammonium bicarbonate.
The potassium salt is one or more of potassium phosphate, potassium dihydrogen phosphate and potassium dihydrogen phosphate.
Example two:
the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate comprises the following raw materials in parts by weight: 2 parts of enterobacter carcinogenes, 2 parts of ferment viable bacteria, 5 parts of plant growth regulator, 7 parts of ammonium salt, 7 parts of potassium salt, 7 parts of plant ash, 4 parts of amino acid zinc, 7 parts of calcium chloride, 7 parts of carbendazim and 27 parts of water.
The rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate comprises the following steps:
s1 preparation of plant growth regulator
One or more raw materials of the brassinolide, the salicylic acid, the jasmonic acid, the paclobutrazol and the polyamine are selected through a mixing container, mixed to prepare the plant growth regulator, and the required amount is weighed for backup.
S2, ammonium and potassium salt preparation
Selecting one or two of ammonium carbonate and ammonium bicarbonate to mix by a mixing container to prepare the required ammonium salt, weighing the required ammonium salt for backup, selecting one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate to mix by the mixing container to prepare the required potassium salt, and weighing the required potassium salt for backup.
S3, weighing and backing up
The method comprises the steps of weighing the required proportion of the enterobacter carcinosus, the live ferment bacteria, the plant ash, the zinc amino acid, the calcium chloride, the carbendazim and the water, and carrying out backup.
S4, mixing the raw materials
The preparation method comprises the steps of putting the backup plant ash, the amino acid zinc, the calcium chloride and the carbendazim into a mixing device, mixing for 4-6 minutes, adding backup water after mixing, heating, stirring and mixing for 5-10 minutes at the temperature of 30-40 ℃, naturally cooling after stirring, and naturally cooling for 25-30 minutes to obtain a mixed solution.
S5, adding growth-promoting rhizobacteria
Adding the rhizosphere growth-promoting bacterium enterobacter tumefaciens and the ferment viable bacteria into the mixed solution in the mixing equipment, mixing, and finally obtaining the finished product of rhizosphere growth-promoting bacterium seed soaking solution.
S6, selecting seeds
And selecting seeds with full grains, no wormholes and no cracks.
S7 seed soaking
Soaking the seeds in the seed soaking liquid containing the rhizosphere growth promoting bacteria for 2-3 hours, taking out the seeds after the seed soaking is finished, and drying or airing the seeds to the extent that no liquid drops exist on the surfaces of the seeds, thus completing the seed soaking process.
The plant growth regulator is prepared by mixing one or more of brassinolide, salicylic acid, jasmonic acid, paclobutrazol and polyamine.
The ammonium salt is one or two of ammonium carbonate and ammonium bicarbonate.
The potassium salt is one or more of potassium phosphate, potassium dihydrogen phosphate and potassium dihydrogen phosphate.
Example three:
the rhizosphere growth-promoting bacteria seed soaking method for improving the germination rate comprises the following raw materials in parts by weight: 3 parts of enterobacter carcinogenes, 3 parts of ferment viable bacteria, 6 parts of plant growth regulator, 10 parts of ammonium salt, 10 parts of potassium salt, 10 parts of plant ash, 5 parts of amino acid zinc, 10 parts of calcium chloride, 10 parts of carbendazim and 30 parts of water.
The rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate comprises the following steps:
s1 preparation of plant growth regulator
One or more raw materials of the brassinolide, the salicylic acid, the jasmonic acid, the paclobutrazol and the polyamine are selected through a mixing container, mixed to prepare the plant growth regulator, and the required amount is weighed for backup.
S2, ammonium and potassium salt preparation
Selecting one or two of ammonium carbonate and ammonium bicarbonate to mix by a mixing container to prepare the required ammonium salt, weighing the required ammonium salt for backup, selecting one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate to mix by the mixing container to prepare the required potassium salt, and weighing the required potassium salt for backup.
S3, weighing and backing up
The method comprises the steps of weighing the required proportion of the enterobacter carcinosus, the live ferment bacteria, the plant ash, the zinc amino acid, the calcium chloride, the carbendazim and the water, and carrying out backup.
S4, mixing the raw materials
The preparation method comprises the steps of putting the backup plant ash, the amino acid zinc, the calcium chloride and the carbendazim into a mixing device, mixing for 4-6 minutes, adding backup water after mixing, heating, stirring and mixing for 5-10 minutes at the temperature of 30-40 ℃, naturally cooling after stirring, and naturally cooling for 25-30 minutes to obtain a mixed solution.
S5, adding growth-promoting rhizobacteria
Adding the rhizosphere growth-promoting bacterium enterobacter tumefaciens and the ferment viable bacteria into the mixed solution in the mixing equipment, mixing, and finally obtaining the finished product of rhizosphere growth-promoting bacterium seed soaking solution.
S6, selecting seeds
And selecting seeds with full grains, no wormholes and no cracks.
S7 seed soaking
Soaking the seeds in the seed soaking liquid containing the rhizosphere growth promoting bacteria for 2-3 hours, taking out the seeds after the seed soaking is finished, and drying or airing the seeds to the extent that no liquid drops exist on the surfaces of the seeds, thus completing the seed soaking process.
The plant growth regulator is prepared by mixing one or more of brassinolide, salicylic acid, jasmonic acid, paclobutrazol and polyamine.
The ammonium salt is one or two of ammonium carbonate and ammonium bicarbonate.
The potassium salt is one or more of potassium phosphate, potassium dihydrogen phosphate and potassium dihydrogen phosphate.
The invention has the beneficial effects that: the rhizosphere growth-promoting bacterium enterobacter carcinosus is added, so that the division speed and the water absorption speed of embryo cells can be greatly improved, the germination speed of seeds is increased, the germination rate of the seeds is increased, the rhizosphere growth-promoting bacterium seed soaking liquid also contains nutrient components required by a plurality of seeds in the germination process, the requirement for improving the intake of required elements of the seeds in the germination process can be met, the germination rate of the seeds is increased, meanwhile, some insect eggs and viruses can be killed, the germination rate of the seeds is further increased, and the phenomenon that the seeds are killed due to the loss of activity of the insect eggs or the viruses is avoided.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. The rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate is characterized in that the rhizosphere growth-promoting bacterium seed soaking liquid comprises the following raw materials in parts by weight: 1-3 parts of enterobacter carcinogenes, 1-3 parts of enzyme live bacteria, 3-6 parts of plant growth regulator, 5-10 parts of ammonium salt, 5-10 parts of potassium salt, 5-10 parts of plant ash, 3-5 parts of amino acid zinc, 5-10 parts of calcium chloride, 5-10 parts of carbendazim and 25-30 parts of water;
the rhizosphere growth-promoting bacterium seed soaking method for improving the germination rate comprises the following steps:
s1 preparation of plant growth regulator
Selecting one or more raw materials of lactone of rape, salicylic acid, jasmonic acid, paclobutrazol and polyamine through a mixing container, mixing to prepare a plant growth regulator, and weighing the required amount for backup;
s2, ammonium and potassium salt preparation
Selecting one or two of ammonium carbonate and ammonium bicarbonate to mix through a mixing container to prepare required ammonium salt, weighing the required ammonium salt for backup, selecting one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate to mix through the mixing container to prepare required potassium salt, weighing the required potassium salt for backup;
s3, weighing and backing up
Weighing the required proportion of the enterobacter carcinogenes, the live ferment bacteria, the plant ash, the zinc amino acid, the calcium chloride, the carbendazim and the water by using the equipment for backup;
s4, mixing the raw materials
The preparation method comprises the steps of putting the backup plant ash, the amino acid zinc, the calcium chloride and the carbendazim into a mixing device, mixing for 4-6 minutes, adding backup water after mixing, heating, stirring and mixing, and naturally cooling after stirring to obtain a mixed solution;
s5, adding growth-promoting rhizobacteria
Adding rhizosphere growth-promoting bacteria enterobacter tumefaciens and enzyme live bacteria into the mixed solution in the mixing equipment, and mixing to finally obtain a finished product of rhizosphere growth-promoting bacteria seed soaking solution;
s6, selecting seeds
Selecting seeds with full grains, no wormholes and no cracks;
s7 seed soaking
And (3) soaking the seeds in the seed soaking solution containing the rhizosphere growth-promoting bacteria, taking out the seeds after the seed soaking solution is finished, and drying or airing the seeds to finish the seed soaking process.
2. The germination rate improving rhizosphere growth promoting bacteria seed soaking method according to claim 1, wherein the plant growth regulator is formed by mixing one or more of brassinolide, salicylic acid, jasmonic acid, paclobutrazol and polyamine.
3. The method for improving germination rate of rhizosphere growth-promoting bacteria according to claim 1, wherein the ammonium salt is one or a mixture of ammonium carbonate and ammonium bicarbonate.
4. The method for soaking the rhizosphere growth-promoting bacteria to improve the germination rate as claimed in claim 1, wherein the potassium salt is one or more of potassium phosphate, potassium monohydrogen phosphate and potassium dihydrogen phosphate.
5. The method for soaking the rhizosphere growth-promoting bacteria to improve the germination rate as claimed in claim 1, wherein the temperature for heating, mixing and stirring in the step S4 is 30-40 ℃, and the stirring time is 5-10 minutes.
6. The method for improving germination rate of rhizosphere growth-promoting bacteria seed soaking according to claim 1, wherein the natural cooling time in step S4 is 25-30 minutes.
7. The method for soaking the rhizosphere growth-promoting bacteria to improve the germination rate as claimed in claim 1, wherein the seed soaking time of the step S7 is 2-3 hours.
8. The method for improving germination rate of rhizosphere growth-promoting bacteria seed soaking according to claim 1, wherein the drying or airing degree in step S7 is no liquid drop on the surface of the seed.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101984067A (en) * | 2010-10-19 | 2011-03-09 | 中国农业大学 | Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria |
| CN104430540A (en) * | 2014-12-31 | 2015-03-25 | 吴中区金庭小美华家庭农场 | Environment-friendly seed treating agent for increasing seed germination rate and preparation method thereof |
| CN104805045A (en) * | 2015-05-05 | 2015-07-29 | 中国科学院烟台海岸带研究所 | Plant growth-promoting rhizobacteria (PGPR) and application thereof |
| CN106472568A (en) * | 2015-08-28 | 2017-03-08 | 深圳华大基因研究院 | The application in terms of the economical character improving plant of five kinds of plant growth-promoting rhizobacterias |
| CN107032884A (en) * | 2017-04-24 | 2017-08-11 | 江西省农业科学院 | One cultivate peanut seed-soaking liquid and use its whole pod seed-soaking method |
| CN108934851A (en) * | 2018-07-19 | 2018-12-07 | 山东祥维斯生物科技股份有限公司 | A kind of saline-alkali tolerant degeneration-resistant paddy seedling culture method coldly |
-
2020
- 2020-12-02 CN CN202011392080.1A patent/CN112514595A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101984067A (en) * | 2010-10-19 | 2011-03-09 | 中国农业大学 | Method of detecting growth promoting effect of plant rhizosphere growth promoting bacteria |
| CN104430540A (en) * | 2014-12-31 | 2015-03-25 | 吴中区金庭小美华家庭农场 | Environment-friendly seed treating agent for increasing seed germination rate and preparation method thereof |
| CN104805045A (en) * | 2015-05-05 | 2015-07-29 | 中国科学院烟台海岸带研究所 | Plant growth-promoting rhizobacteria (PGPR) and application thereof |
| CN106472568A (en) * | 2015-08-28 | 2017-03-08 | 深圳华大基因研究院 | The application in terms of the economical character improving plant of five kinds of plant growth-promoting rhizobacterias |
| CN107032884A (en) * | 2017-04-24 | 2017-08-11 | 江西省农业科学院 | One cultivate peanut seed-soaking liquid and use its whole pod seed-soaking method |
| CN108934851A (en) * | 2018-07-19 | 2018-12-07 | 山东祥维斯生物科技股份有限公司 | A kind of saline-alkali tolerant degeneration-resistant paddy seedling culture method coldly |
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