CN106472568A - The application in terms of the economical character improving plant of five kinds of plant growth-promoting rhizobacterias - Google Patents
The application in terms of the economical character improving plant of five kinds of plant growth-promoting rhizobacterias Download PDFInfo
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Abstract
The invention provides the application in terms of the economical character improving plant of five kinds of plant growth-promoting rhizobacterias.Specifically, the invention discloses a kind of purposes of plant growth-promoting rhizobacteria, it is used for preparing Agrotechnical formulation, described Agrotechnical formulation is used for improving the economical character of plant, and wherein said plant growth-promoting rhizobacteria is selected from the group：Yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof.Five kinds of bacterium of the present invention and combinations thereof are able to (i) and promote seed to sprout；(ii) improve plant height；(iii) root system development (iv) is promoted to increase leaf area；V () increases tiller number；(vi) yield, therefore great application prospect are improved.
Description
Technical field
The invention belongs to biotechnology and breeding field, in particular it relates to application in terms of the economical character improving plant for the plant growth-promoting rhizobacteria (yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas)).
Background technology
Chemical fertilizer plays great function in modern agricultural production.But it is as the increase of fertilizer application amount, its utilization rate reduces year by year.Pesticide and the residual of chemical fertilizer, the substantial amounts of harmful substance to Environment release, pollute soil, water source and food.The mankind are separated and living environment constitutes great threat it is desirable to preserve the ecological environment and the cry of production safety food is increasingly strong.Therefore, develop the new source of manure substituting chemical fertilizer, be the task of top priority with adapt to develop green agriculture and pollution-free food.And microbial manure can make invalid nutrition validation in soil, prevention and control corps diseases, reduce the use of pesticide and chemical fertilizer, be the fundamental way solving soil, water source and food pollution, be generally considered a kind of environmental friendliness, the method for cost-effective raising crop yield.
Plant growth-promoting rhizobacteria (Plant growth promoting rhizobacteria, PGPR it is) that a class energy high density colonizes the microorganism in plant rhizosphere, have Suppressing phytopathogens, rhizosphere harmful microorganism concurrently, and promote plant growing and increase the effect of crop yield.As the valuable source storehouse of bio-feritlizer and biological pesticide, very important effect has been arrived in the research of PGPR and application.However, most plant growth-promoting rhizobacteria promote the effect of growth and development of plants and inconspicuous it is difficult to obtain consumer's accreditation；Some plant growth-promoting rhizobacterias are also had only just to have growth-promoting functions to one or several plants, thus being difficult to be widely applied..
Therefore, this area in the urgent need to develop plant growth-promoting rhizobacteria, it can not only be obviously promoted the growth promoter of broad-spectrum plant, can also prepare corresponding Agrotechnical formulation, is widely used in microbial manure field.
Content of the invention
It is an object of the invention to provide five kinds of plant growth-promoting rhizobacterias, they can not only be obviously promoted growth and development of plants, be also used as Agrotechnical formulation, be widely used in microbial manure field.
First aspect present invention provides a kind of purposes of plant growth-promoting rhizobacteria, is used for preparing Agrotechnical formulation, and described Agrotechnical formulation is used for improving the economical character of plant, and wherein said plant growth-promoting rhizobacteria is selected from the group：Yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes yellow soil source bacterium (Flavisolibacter).
In another preference, described yellow soil source bacterium (Flavisolibacter) is selected from the group：Radix Ginseng khaki soil source bacterium (Flavisolibacter ginsengisoli), Radix Ginseng ground yellow soil source bacterium (Flavisolibacter ginsengiterrae), Flavisolibacter rigui, Flavisolibacter sediminifilum or a combination thereof.
In another preference, described yellow soil source bacterium (Flavisolibacter) is selected from the group：F.ginsengisoli GS643, F.ginsengiterrae GS492, F.rigui 02SUJ3, F.sediminifilum HU1-JC5 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes Ferruginibacter.
In another preference, described Ferruginibacter bacterium is selected from the group：Ferruginibacter alkalilentus, Ferruginibacter lapsinanis, Ferruginibacter yonginensis, Ferruginibacter paludis, Ferruginibacter profundus or a combination thereof.
In another preference, described Ferruginibacter bacterium is selected from the group：F.alkalilentus HU1-GD23, F.alkalilentus HU1-GB11, F.alkalilentus HU1-ID41, F.lapsinanis HU1-HG42, F.yonginensis HME8442, F.paludis HME8881, F.profundus DS48-5-3 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes Lenze Salmonella (Lentzea).
In another preference, described Lenze Salmonella (Lentzea) is selected from the group：Micro- white Lenze Salmonella (Lentzea albida), white silk Lenze Salmonella (Lentzea albidocapillata), California Lenze Salmonella (Lentzea californiensis), yellow wart Lenze Salmonella (Lentzea flaviverrucosa), Jiangxi Lenze Salmonella (Lentzea jiangxiensis), Kentucky Lenze Salmonella (Lentzea kentuckyensis), purple Lenze Salmonella (Lentzea violacea), prestige Weida lake Lenze Salmonella (Lentzea waywayandensis), or a combination thereof.
In another preference, described Lenze Salmonella (Lentzea) is selected from the group：L.albida NA 944235、L.albida R10069、L.albidocapillata IMMIB D-958、L.albidocapillata 4.215、L.californiensis IMRU 550、L.flaviverrucosa 5-125、L.flaviverrucosa 174513、L.flaviverrucosa 6-4-7T、L.jiangxiensis FXJ1.034、L.kentuckyensis LDDC 2876-05、L.violacea LM 036、L.violacea CAS 5-124、L.violacea 173536、L.waywayandensis LL-37Z-15、L.waywayandensis 173919、L.waywayandensis 173629、Or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes soil redness rod bacterium (Solirubrobacter).
In another preference, described soil redness rod bacterium (Solirubrobacter) is selected from the group：Solirubrobacter ginsenosidimutans, Bao Ershi soil redness rod bacterium (Solirubrobacter pauli), Solirubrobacter phytolaccae, soil soil redness rod bacterium (Solirubrobacter soli), Solirubrobacter taibaiensis or a combination thereof.
In another preference, described soil redness rod bacterium (Solirubrobacter) is selected from the group：S.ginsenosidimutans BXN5-15, S.pauli B33D1, S.phytolaccae GTGR-8, S.soli Gsoil 355, S.taibaiensis GTJR-20 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes autochthonal Zymomonas mobiliss (Terrimonas).
In another preference, described autochthonal Zymomonas mobiliss (Terrimonas) are selected from the group：Terrimonas aquatica, Terrimonas arctica, the embroidery autochthonal Zymomonas mobiliss of color (Terrimonas ferruginea), the autochthonal Zymomonas mobiliss of Resina garciniae color (Terrimonas lutea), Terrimonas pekingensis, Terrimonas rubra or a combination thereof.
In another preference, described autochthonal Zymomonas mobiliss (Terrimonas) are selected from the group：T.aquatica RIB1-6, T.arctica R9-86, T.ferruginea 3576, T.lutea DY, T.pekingensis QH, T.rubra M-8 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria is included selected from one or more of table 6.
In another preference, described plant growth-promoting rhizobacteria is selected from table 6, and from identical or different genus.
In another preference, the described economical character improving plant includes：I () promotes seed to sprout；(ii) improve plant height；(iii) promote root system development；(iv) increase leaf area；V () increases tiller number；(vi) improve yield.
In another preference, described raising yield includes fresh weight and/or the dry weight improving plant.
In another preference, described plant includes：Grass family、Typhaceae、Pteridiaceae、Compositae、Labiatae、Liliaceae、Amaryllidaceae、Boraginaceae、Araeceae、Umbelliferae、Cruciferae、Primulaceae、Polygonaceae、Chenopodiaceae、Caryophyllaceae、Oenotheraceae、Urticaceae、Plantaginaceae、Myruca ceas、Moraceae、Cannabaceae、Saxifragaceae、Rosaceae、Pulse family、Pteridiaceae、Violaceae、Yin Chi Caulis et Folium Lactucae sativae section、Amaranthaceae、Fagaceae、Chlorella section、Theaceae、Rubiaceae、Sterculiaceae、Pinaceae、Cucurbitaceae、Flacourtiaceae、Podocarpaceae、Betulaceae、Juglandaceae、Piperaceae、Magnoliaceae、Tooth bacterium section、Auriculariaceae、Tricholomataceae、Agaricaceae、Russulaceae、Ericaceae、Rosaceae、Actinidiaceae、Aizoaceae、Vitaceae、Annonaceae、Begoniaceae、Pineapple family、Capparidaceae、Ginkgoaceae、Illiciaceae、Zingiberaceae、Punicaceae、Ranunculaceae、Zhu Tao section、Berberidaceae、Rutaceae、Solanaceae、Papaveraceae、Verenaceae、Pyrolaceae、Commelianaceae、Thymelaeceae、Loranthaceae、Trailing plants section、Saururaceae、Crassulaceae、Lin Xiang section、Portulacaceae、Alismataceae、Malvaceae、Scrophulariaceae、Bignoniaceae、Menispermaceae、Lygodiaceae、Acanthaceae、Convolvulaceae、Polyporaceae、Sapindaceae、Cupressaceae、Simarubaceae、Euphorbiaceae、Meliaceae、Combretum Racemosum、Caprifoliaceae、Ramulus et Folium Tamariciss section、Orobanchaceae、Rush family、Hamamelidaceae、Gentianaceae、Tiliaceae、Acoraceae、Aquifoliaceae、Plantaginaceae、Anacardiaceae、Schisandraceae、Araliaceae、Bombacaceae、Aristolochiaceae、Oleaceae、Palmae、Nymphaeceae、Sandalwood plants、Or a combination thereof.
In another preference, described plant is Caulis et Folium Lactucae Sativae, Oryza sativa L., Semen Tritici aestivi, Semen Maydiss, Semen arachidis hypogaeae, Sorghum vulgare Pers., Semen sojae atricolor, Rhizoma Solani tuber osi, Semen Fagopyri Esculenti, Herba chenopodii, Ji, Fructus Piperiss, anise, Fructus Foeniculi, Fructus Persicae, Fructus Pruni, pears, Fructus Mali pumilae, Fructus Musae, hedgehog hydnum, Auricularia, Rhizoma Dioscoreae, Fructus Crataegi, Radix Ginseng, Radix Angelicae Sinensis, Fructus Lycopersici esculenti, Fructus Capsici, Fructus Solani melongenae, Radix Dauci Sativae, Caulis et Folium Brassicae capitatae, Brassica oleracea L. var. botrytis L., Chinese cabbage, Plantula Brassicae chinensis, Brassica campestris L, Herba Spinaciae, Caulis et Folium Brassicae junceae, Semen Pisi sativi, Fructus Cucurbitae moschatae, Fructus Cucumidis sativi, Citrullus vulgariss, Fructus Melo, asparaguss, Bulbus Allii Cepae or a combination thereof.
Second aspect present invention provides a kind of Agrotechnical formulation for improving plant agronomic character, and described Agrotechnical formulation includes (a) plant growth-promoting rhizobacteria of safe and effective amount；(b) auxiliary agent or agronomically acceptable carrier；Wherein, described plant growth-promoting rhizobacteria is selected from the group：Yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes yellow soil source bacterium (Flavisolibacter).
In another preference, described yellow soil source bacterium (Flavisolibacter) is selected from the group：Radix Ginseng khaki soil source bacterium (Flavisolibacter ginsengisoli), Radix Ginseng ground yellow soil source bacterium (Flavisolibacter ginsengiterrae), Flavisolibacter rigui, Flavisolibacter sediminifilum or a combination thereof.
In another preference, described yellow soil source bacterium (Flavisolibacter) is selected from the group：F.ginsengisoli GS643, F.ginsengiterrae GS492, F.rigui 02SUJ3, F.sediminifilum HU1-JC5 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes Ferruginibacter.
In another preference, described Ferruginibacter bacterium is selected from the group：Ferruginibacter alkalilentus, Ferruginibacter lapsinanis, Ferruginibacter yonginensis, Ferruginibacter paludis, Ferruginibacter profundus or a combination thereof.
In another preference, described Ferruginibacter bacterium is selected from the group：F.alkalilentus HU1-GD23, F.alkalilentus HU1-GB11, F.alkalilentus HU1-ID41, F.lapsinanis HU1-HG42, F.yonginensis HME8442, F.paludis HME8881, F.profundus DS48-5-3 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes Lenze Salmonella (Lentzea).
In another preference, described Lenze Salmonella (Lentzea) is selected from the group：Micro- white Lenze Salmonella (Lentzea albida), white silk Lenze Salmonella (Lentzea albidocapillata), California Lenze Salmonella (Lentzea californiensis), yellow wart Lenze Salmonella (Lentzea flaviverrucosa), Jiangxi Lenze Salmonella (Lentzea jiangxiensis), Kentucky Lenze Salmonella (Lentzea kentuckyensis), purple Lenze Salmonella (Lentzea violacea), prestige Weida lake Lenze Salmonella (Lentzea waywayandensis), or a combination thereof.
In another preference, described Lenze Salmonella (Lentzea) is selected from the group：L.albida NA 944235、L.albida R10069、L.albidocapillata IMMIB D-958、L.albidocapillata 4.215、L.californiensis IMRU 550、L.flaviverrucosa 5-125、L.flaviverrucosa 174513、L.flaviverrucosa 6-4-7T、L.jiangxiensis FXJ1.034、L.kentuckyensis LDDC 2876-05、L.violacea LM 036、L.violacea CAS 5-124、L.violacea 173536、L.waywayandensis LL-37Z-15、L.waywayandensis 173919、L.waywayandensis 173629、Or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes soil redness rod bacterium (Solirubrobacter).
In another preference, described soil redness rod bacterium (Solirubrobacter) is selected from the group：Solirubrobacter ginsenosidimutans, Bao Ershi soil redness rod bacterium (Solirubrobacter pauli), Solirubrobacter phytolaccae, soil soil redness rod bacterium (Solirubrobacter soli), Solirubrobacter taibaiensis or a combination thereof.
In another preference, described soil redness rod bacterium (Solirubrobacter) is selected from the group：S.ginsenosidimutans BXN5-15, S.pauli B33D1, S.phytolaccae GTGR-8, S.soli Gsoil 355, S.taibaiensis GTJR-20 or a combination thereof.
In another preference, described plant growth-promoting rhizobacteria includes autochthonal Zymomonas mobiliss (Terrimonas).
In another preference, described autochthonal Zymomonas mobiliss (Terrimonas) are selected from the group：Terrimonas aquatica, Terrimonas arctica, the embroidery autochthonal Zymomonas mobiliss of color (Terrimonas ferruginea), the autochthonal Zymomonas mobiliss of Resina garciniae color (Terrimonas lutea), Terrimonas pekingensis, Terrimonas rubra or a combination thereof.
In another preference, described autochthonal Zymomonas mobiliss (Terrimonas) are selected from the group：T.aquatica RIB1-6, T.arctica R9-86, T.ferruginea 3576, T.lutea DY, T.pekingensis QH, T.rubra M-8 or a combination thereof.
In another preference, described auxiliary agent or agronomically acceptable carrier include additive, binding agent and/or protective agent.
In another preference, described additive is silicon dioxide, Kaolin, powdered rice hulls, wheat husk powder, powder of straw or a combination thereof.
In another preference, described binding agent is bentonite, water-soluble vitamins polysaccharide or a combination thereof.
In another preference, described protective agent is glycerol, defatted milk, vegetable oil, sodium alginate, shitosan or a combination thereof.
In another preference, described Agrotechnical formulation contains 1 × 10-1 × 1020Cfu/mL or 1 × 10-1 × 1020Cfu/g plant growth-promoting rhizobacteria, preferably 1 × 104-1×1015Cfu/mL or cfu/g plant growth-promoting rhizobacteria, by cumulative volume or the gross weight meter of described Agrotechnical formulation.
In another preference, in described Agrotechnical formulation, containing 0.0001-99wt%, the preferably plant growth-promoting rhizobacteria described in 0.1-90wt%, with the gross weight meter of described Agrotechnical formulation.
In another preference, described Agrotechnical formulation also includes fertilizer, and described fertilizer is selected from the group：Nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, compound fertilizer or a combination thereof.
In another preference, described Agrotechnical formulation also includes other plant growth regulator, and described plant growth regulator is selected from the group：Humic acidss, chlorocholine chloride, Yield-increasing Hormone, naphthalene acetic acid or a combination thereof.
In another preference, the dosage form of described Agrotechnical formulation is selected from the group：Wettable powder, water dispersible granules, suspending agent, aqueous emulsion, granule, seed coat agent or a combination thereof.
Third aspect present invention provides a kind of method promoting growth and development of plants, and methods described includes step：Apply the Agrotechnical formulation described in second aspect present invention in plant root.
In another preference, described application process includes：Seed dressing, seed soaking, pouring root, spread manuer in holes, rush and apply, drip irrigation, viscous root, spread fertilizer over the fields.
Fourth aspect present invention provides the preparation method of Agrotechnical formulation described in second aspect present invention, and methods described includes step：Described to (a) plant growth-promoting rhizobacteria is mixed with (b) auxiliary agent or agronomically acceptable carrier, thus forming the Agrotechnical formulation described in second aspect present invention.
It should be understood that within the scope of the present invention, can be combined with each other between above-mentioned each technical characteristic of the present invention and each technical characteristic specifically describing in below (eg embodiment), thus constituting new or preferred technical scheme.As space is limited, this is no longer going to repeat them.
Brief description
Fig. 1 shows the impact that under laboratory condition, yellow soil source bacterium sprouts to millet.
Fig. 2 shows the impact that under laboratory condition, Ferruginibacter bacterium sprouts to millet.
Fig. 3 shows the impact that under laboratory condition, Lenze Salmonella sprouts to millet.
Fig. 4 shows the impact that under laboratory condition, soil redness rod bacterium sprouts to millet.
Fig. 5 shows the impact that under laboratory condition, autochthonal Zymomonas mobiliss are sprouted to millet.
Fig. 6 shows the impact that under laboratory condition, strain combinations are sprouted to millet.
Fig. 7 shows the impact to millet growth-promoting functions for the yellow soil source bacterium under condition of pot.
Fig. 8 shows the impact to millet growth-promoting functions for the Ferruginibacter bacterium under condition of pot.
Fig. 9 shows the impact to millet growth-promoting functions for the Lenze Salmonella under condition of pot.
Figure 10 shows the soil redness impact to millet growth-promoting functions for the rod bacterium under condition of pot.
Figure 11 shows the impact to millet growth-promoting functions for the autochthonal Zymomonas mobiliss under condition of pot.
Figure 12 shows the impact to millet growth-promoting functions for the strain combinations under condition of pot.
Figure 13 shows the impact to Semen Maydiss growth-promoting functions for the yellow soil source bacterium under condition of pot.
Figure 14 shows the impact to Semen Maydiss growth-promoting functions for the Ferruginibacter bacterium under condition of pot.
Figure 15 shows the impact to Semen Maydiss growth-promoting functions for the Lenze Salmonella under condition of pot.
Figure 16 shows the soil redness impact to Semen Maydiss growth-promoting functions for the rod bacterium under condition of pot.
Figure 17 shows the impact to Semen Maydiss growth-promoting functions for the autochthonal Zymomonas mobiliss under condition of pot.
Figure 18 shows the impact to Semen Maydiss growth-promoting functions for the strain combinations under condition of pot.
Figure 19 shows the impact to Semen Tritici aestivi growth-promoting functions for the yellow soil source bacterium under condition of pot.
Figure 20 shows the impact to Semen Tritici aestivi growth-promoting functions for the Ferruginibacter bacterium under condition of pot.
Figure 21 shows the impact to Semen Tritici aestivi growth-promoting functions for the Lenze Salmonella under condition of pot.
Figure 22 shows the soil redness impact to Semen Tritici aestivi growth-promoting functions for the rod bacterium under condition of pot.
Figure 23 shows the impact to Semen Tritici aestivi growth-promoting functions for the autochthonal Zymomonas mobiliss under condition of pot.
Figure 24 shows the impact to Semen Tritici aestivi growth-promoting functions for the strain combinations under condition of pot.
Figure 25 shows the impact to millet growth-promoting functions for the yellow soil source bacterium under field condition.
Figure 26 shows the impact to millet growth-promoting functions for the Ferruginibacter bacterium under field condition.
Figure 27 shows the impact to millet growth-promoting functions for the Lenze Salmonella under field condition.
Figure 28 shows the soil redness impact to millet growth-promoting functions for the rod bacterium under field condition.
Figure 29 shows the impact to millet growth-promoting functions for the autochthonal Zymomonas mobiliss under field condition.
Figure 30 shows the impact to millet growth-promoting functions for the strain combinations under field condition.
Figure 31 shows the impact to Fructus Lycopersici esculenti growth-promoting functions for the yellow soil source bacterium under field condition.
Figure 32 shows the impact to Fructus Lycopersici esculenti growth-promoting functions for the Ferruginibacter bacterium under field condition.
Figure 33 shows the impact to Fructus Lycopersici esculenti growth-promoting functions for the Lenze Salmonella under field condition.
Figure 34 shows the soil redness impact to Fructus Lycopersici esculenti growth-promoting functions for the rod bacterium under field condition.
Figure 35 shows the impact to Fructus Lycopersici esculenti growth-promoting functions for the autochthonal Zymomonas mobiliss under field condition.
Figure 36 shows the impact to Fructus Lycopersici esculenti growth-promoting functions for the strain combinations under field condition.
Wherein, different on error bar letter representation significant difference (p<0.05).
Specific embodiment
The present inventor is by extensively in-depth study, carry out preliminary screening by grand genome analysises, through experimental verification, it is surprised to find that, yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) can significantly improve the economical character of plant, be embodied in：I () promotes seed to sprout；(ii) improve plant height；(iii) root system development (iv) is promoted to increase leaf area；V () increases tiller number；(vi) improve yield.Therefore above-mentioned five kinds of plant growth-promoting rhizobacterias have extensive prospect in terms of promoting growth and development of plants, can serve as Agrotechnical formulation.On this basis, the present inventor completes the present invention.
The plant growth-promoting rhizobacteria of the present invention and its application
As used herein, described " plant growth-promoting rhizobacteria of the present invention " refers to one or more mixing of multiple bacterium in yellow soil source bacterium, Ferruginibacter bacterium, the mixing of one or more Pseudomonas of Lenze Salmonella, soil redness rod bacterium and five kinds of Pseudomonas of autochthonal Zymomonas mobiliss or each Pseudomonas.
Wherein, described yellow soil source bacterium is gram positive bacteria, does not produce spore, has xanthein.
Described Ferruginibacter bacterium is bacilluss, gram positive bacteria, and forming bacterium colony is in rust, can produce Liquor Hydrogen Peroxide enzyme and oxidase.
Described Lenze Salmonella is a kind of ray fungus that loves intermediate temperature, can form substantial amounts of aerial hyphae.
Described soil redness rod bacterium is gram positive bacteria, does not produce spore, shaft-like.
Described autochthonal Zymomonas mobiliss also belong to a genus of Chitinophagaceae section, strict aerobes, and Gram’s staining is positive, shaft-like, can produce oxidase and faint Liquor Hydrogen Peroxide enzyme.
Described five kinds of plant growth-promoting rhizobacterias can significantly improve the economical character of plant, is embodied in：I () promotes seed to sprout；(ii) improve plant height；(iii) root system development (iv) is promoted to increase leaf area；V () increases tiller number；(vi) improve yield.
Agrotechnical formulation
The active substance (yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof) of the present invention can be prepared into Agrotechnical formulation, such as solution, Emulsion, suspensoid, powder, foam, paste, granule, aerosol, the material of natural and synthesis being impregnated with active substance, the microcapsule in polymer, the coating materials for seed in a conventional way.
These preparations can use known method to produce, for example, active substance is mixed with expanding agent, these expand agent is exactly liquid or liquefied gas or solid diluent or carrier, and can be emulsifying agent and/or dispersant and/or formation of foam agent arbitrarily from surfactant.For example when using water as expanding agent, organic solvent also is used as auxiliary agent.
When making diluent or carrier with liquid flux, substantially suitably, such as：Arene, such as dimethylbenzene, toluene or alkylnaphthalene；The fragrance of chlorination or the fat hydrocarbon of chlorination, such as chlorobenzene, vinyl chloride or dichloromethane；Fat hydrocarbon, such as hexamethylene or paraffin, such as mineral oil fractions；Alcohols, such as ethanol or ethylene glycol and their ether and lipid；Ketone, such as acetone, butanone, methyl iso-butyl ketone (MIBK) or cyclohexanone；Or the polar solvent being of little use, such as dimethylformamide and dimethyl sulfoxide, Yi Jishui.
Diluent or carrier with regard to liquefied gas is said, refers to will become at normal temperatures and pressures the liquid of gas, such as aerosol propellants, such as the hydro carbons of halogenation and butane, propane, nitrogen and carbon dioxide.
Solid carrier can use the natural mineral matter of grinding, such as Kaolin, clay, Talcum, quartz, active hargil, montmorillonite, or kieselguhr, and the mineral of grinding synthesis, the silicic acid of such as high degree of dispersion, aluminium oxide and silicate.Solid carrier for granule is natural zircon that pulverize and classification, such as calcite, marble, Pumex, meerschaum and dolomite, and the granule of inorganic and organic coarse powder synthesis, with organic material such as wood sawdust, Exocarpium cocois (Cocos nucifera L), granule of maize cob and tobacco stems etc..
Non-ionic and anion emulsifying row can be used as emulsifying agent and/or formation of foam agent.Such as polyoxyethylene-fatty esters of gallic acid, polyoxyethylene-fatty alcohol ethers, such as alkaryl Polyethylene Glycol ethers, alkyl sulfonates, alkylsurfuric acid esters, aryl sulfonic acid esters and albumin hydrolyzate.Dispersant includes, such as lignin sulfite waste liquor and methylcellulose.
Binding agent, such as carboxymethyl cellulose and the polymer with powder, granule or the natural and synthesis of emulsion form, such as arabic gum, polyvinyl alcohol and polyvinyl acetate can be used in the formulation.
Can be with coloring agent such as inorganic dyestuff, such as ferrum oxide, oxidation brill and Prussian blue；Organic dyestuff, such as organic dyestuff, such as azo dye or metal phthalcyanine；With use trace nutritional agent, such as ferrum, suddenly, boron, copper, cobalt, salt of aluminum and zinc etc..
In the present invention, described " Agrotechnical formulation " is typically agricultural plant growth regulator, and it contains yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter) and/or autochthonal Zymomonas mobiliss (Terrimonas) as the active component promoting growth and development of plants；And agriculturally acceptable carrier.
As used herein, described " agriculturally acceptable carrier " is for sending yellow soil source bacterium (Flavisolibacter) of the present invention, Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter) and/or autochthonal Zymomonas mobiliss (Terrimonas) in the Pesticide Science of plant acceptable solvent, suspending agent or excipient.Carrier can be liquid or solid.The agriculturally acceptable carrier being applied to the present invention is selected from the group：Water, buffer, DMSO, surfactant such as Tween-20 or a combination thereof.Any agriculturally acceptable carrier well known by persons skilled in the art is used equally in the present invention.
The Agrotechnical formulation of the present invention can be made a kind of mixture with other plant growth regulator and be present in their commercial preparation or from the use dosage form of these preparations preparation, and these other plant growth regulators include (being not limited to)：Humic acidss, chlorocholine chloride, Yield-increasing Hormone, naphthalene acetic acid or a combination thereof.
Additionally, the Agrotechnical formulation of the present invention also can make a kind of mixture with fertilizer being present in their commercial preparation or from the use dosage form of these preparations preparation, these fertilizer are selected from the group；Nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, compound fertilizer or a combination thereof.
The dosage form of Agrotechnical formulation of the present invention can be diversified, as long as the dosage form that active component reaches in plant body effectively can be made all to be possible, in terms of position that is easily prepared and applying, preferred Agrotechnical formulation is a kind of spray, pharmaceutical solutionses or granule.
Agrotechnical formulation of the present invention usually contains the 0.001-99.99wt% accounting for described Agrotechnical formulation gross weight, preferably 0.01-99.9wt%, the more preferably the compounds of this invention of 0.05-90wt%.Commercial preparation or can be changed in wide scope using the concentration of the compounds of this invention in dosage form.Concentration using the compounds of this invention in dosage form can be from 0.0000001-100% (g/v), preferably between 0.0001 and 1% (g/v).
Main advantages of the present invention：
(1) present invention firstly discovers that yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof can significantly improve the economical character of plant, such as promote seed to sprout, improve plant height, promote root system development (as increased root length, improving the density of root length), increase leaf area, increase tiller number, improve yield etc..
(2) present invention first using yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof as Agrotechnical formulation main active, use with the combination of other plant growth regulator or as microbial manure, it is demonstrated experimentally that the Agrotechnical formulation containing yellow soil source bacterium or microbial manure can remarkably promote the growth promoter of plant (as millet, Fructus Lycopersici esculenti, Semen Tritici aestivi, Semen Maydiss etc.).
(3) present invention adopts plant growth-promoting rhizobacteria as the Agrotechnical formulation promoting growth and development of plants, the series of problems that chemical fertilizer brings can be avoided, thus be conducive to the No-harmful apple orchard of plant (as crops), grower without chemical fertilizer or can reduce fertilizer amount, this not only can reduce expenses, and is conducive to the outlet of fruit etc..
Embodiment 1 determines the probioticss promoting growth and development of plants
Pedotheque picks up from Yangling Shaanxi agricultural high technology industry Demonstration Garden (34 ° 16 ' 18 " N/108 ° 4 ' 59 " E) millet experimental plot respectively.When millet harvests，Each strain millet randomly selects 3 plants，Using Bulgarelli etc. (2012) (Bulgarelli,D.,Rott,M.,Schlaeppi,K.,van Themaat,E.V.L.,Ahmadinejad,N.,Assenza,F.,...&Schulze-Lefert,P.(2012).Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota.Nature,488(7409),Method 91-95.) describing collects rhizosphere soil.Meanwhile, 10 parts of soil are taken in not planting plot.The pedotheque taking is put into the collecting pipe after sterilizing, is freezed with dry ice and transport BGI-Shenzhen, be placed in -20 DEG C of cryogenic refrigerators and save backup.
The extraction that every part of sample takes 0.2g to carry out genomic DNA, using for soil((MP Biomedicals, Santa Ana, CA), the description providing with reference to production firm is carried out SPIN test kit.
Expanded using 16S rRNA gene V4-V5 hypervariable region special primer.Primer sequence is as follows：
PCR reaction condition is：94 DEG C of denaturations 4min；Then in 94 DEG C of degeneration 45s, 55 DEG C of annealing 45s, 72 DEG C of extension 1min, circulate 30 times；72 DEG C extend 10min eventually.The PCR primer obtaining utilizes AxyPrepTMPCR cleaning agents box (Axygen Scientific, Inc.US) carries out purification, the operation instructions that concrete steps provide with reference to manufacturer.
UsingSizeSelectTM2% agarose gel carries out purification to the PCR primer comprising joint and Ion Xpress barcode sequence, and is concentrated using AMPure Beads 1.2 × (Beckman Coulter)；Using Ion OneTouch 2TMAnd Ion Template PGMTMOT2 400 test kit (Life Technologies) carries out emulsifying PCR；Using Ion 318TMChip test kit is sequenced in Torrent PGM system.
Sequencing result utilizes PGM software to remove low quality sequence and polyclone sequence, carries out quality control (Schloss, P.D. using Mothur software, Westcott, S.L., Ryabin, T., Hall, J.R., Hartmann, M., Hollister, E.B., ... ＆Weber, C.F. (2009) .Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial com).The high-quality obtaining sequence is carried out cluster and obtains OUT.OTU is associated analyzing with millet yield, find that OTU255, OTU5352, OTU1892, OTU414 and OTU2200 and millet high yield relatedness are stronger, annotated respectively yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter) and autochthonal Zymomonas mobiliss (Terrimonas).
Embodiment 2 is chosen bacterial strain, is prepared Agrotechnical formulation
2.1 bacterium source
The Pseudomonas information that according to embodiments of the present invention 1 obtains, the 16S rRNA gene order of many plants of yellow soil source bacterium is carried out BLAST with OUT255 and compares by inventor, comparison result such as table 1；The 16S rRNA gene order of many plants of Ferruginibacter bacterium is carried out BLAST with OTU5352 compare, comparison result such as table 2；The 16S rRNA gene order of many plants of Lenze Salmonellas is carried out BLAST with OTU1892 compare, comparison result such as table 3；The 16S rRNA gene order of many plants of soil redness rod bacterium is carried out BLAST with OTU414 compare, comparison result such as table 4；The 16S rRNA gene order of many plants of autochthonal Zymomonas mobiliss is carried out BLAST with OTU2200 compare, comparison result such as table 5.
Table 1 OTU255 sequence and 4 plants of yellow soil source bacterium 16S rRNA Gene Partial sequence B LAST comparison results
Table 2 OTU5352 sequence and 7 plants of Ferruginibacter bacterium 16S rRNA Gene Partial sequence B LAST comparison results
Table 3 OTU1892 sequence and 8 plants of Lenze's Salmonella type strain 16S rRNA Gene Partial sequence B LAST comparison results
Table 4 OTU414 sequence and 5 plants of soil redness rod bacterium autochthonal Zymomonas mobiliss 16S rRNA Gene Partial sequence B LAST comparison results
Table 5 OTU2200 sequence and 6 plants of autochthonal Zymomonas mobiliss 16S rRNA Gene Partial sequence B LAST comparison results
Inventor obtains 4 plants of yellow soil source bacterium, 7 plants of Ferruginibacter bacterium, 16 plants of Lenze Salmonellas, 5 plants of soil redness rod bacterium and 6 plants of autochthonal Zymomonas mobiliss from preservation mechanism, and is stored in BGI-Shenzhen.Simultaneously, choose existing Promoting bacteria Bacillus pumilus INR7 (Raj SN, Chaluvaraju G, Amruthesh KN et al.Induction of Growth Promotion and Resistance Against Downy Mildew on Pearl Millet (Pennisetum glaucum) by Rhizobacteria.Plant Disease 2003；87:380-384.) as comparison.
Wherein, the source-information of above-mentioned bacterial strains and control strain (INR7) is as shown in table 6.
Table 6 bacterium source information
*Culture Collection of the Department of Entomology and Plant Pathology,Auburn University(AU),Alabama(AL),United States(US)(Raj SN,Chaluvaraju G,Amruthesh KN et al.Induction of Growth Promotion and Resistance Against Downy Mildew on Pearl Millet(Pennisetum glaucum)by Rhizobacteria.Plant
Disease 2003；87:380-384.)
2.2 bacteria suspensions and the preparation of mycopowder
By each bacterial strain activate after inoculate, access NB culture medium in, 28 ± 2 DEG C, 180r/min shaken cultivation obtain bacterial strain fermentation liquor.Fermentation liquid is centrifuged 15min through 4200r/min, supernatant is moved and abandons.The thalline obtaining is suspended in physiological saline solution (NSS), counts the concentration of bacteria suspension using blood counting chamber microscopy, be adjusted to 5 × 108Individual/ml is standby；Or the thalline obtaining is dried under the conditions of low-temperature negative-pressure, obtain corresponding mycopowder.
The preparation of 2.3 Agrotechnical formulations
The formula of Agrotechnical formulation is as follows：
Bacterium group in formula 1-4 is divided into single bacteria component, contains F.ginsengisoli GS643, F.ginsengiterrae GS492, F.rigui 02SUJ3, F.sediminifilum HU1-JC5 respectively.
The bacterium group of formula 5 is divided into the mixture of above-mentioned 4 bacterium, and (weight is than for 1:1:1:1).
The bacterium group of formula 6 be divided into F.ginsengisoli GS643, the 1 of F.ginsengiterrae GS492:The mixture of 1 (wt)；
The bacterium group of formula 7 is divided into the equal proportion mixture of the bacterium of two Pseudomonas：As yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea). it is combined between any two kinds of Pseudomonas in soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas).
The bacterium group of formula 8 is divided into the equal proportion mixture of the bacterium of 5 Pseudomonas：Yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, the mixture of Lenze Salmonella (Lentzea), soil redness rod bacterium (Solirubrobacter) and autochthonal Zymomonas mobiliss (Terrimonas).
Bacterium group in formula 9-15 is divided into single bacteria component, contains respectively：F.alkalilentus HU1-GD23、F.alkalilentus HU1-GB11、F.alkalilentus HU1-ID41、F.lapsinanis HU1-HG42、F.yonginensis HME8442、F.paludis HME8881、F.profundus DS48-5-3.
The bacterium group of formula 16 is divided into the mixture of above-mentioned 7 bacterium, and (weight is than for 1:1:1:1:1:1:1).
The bacterium group of formula 17 be divided into F.alkalilentus HU1-GD23, the 1 of F.alkalilentus HU1-GB11:The mixture of 1 (wt)；
Bacterium group in formula 18-33 is divided into single bacteria component, contains respectively：L.albida NA 944235、L.albida R10069、L.albidocapillata IMMIB D-958、L.albidocapillata 4.215、L.californiensis IMRU 550、L.flaviverrucosa 5-125、L.flaviverrucosa 174513、L.flaviverrucosa 6-4-7T、L.jiangxiensis FXJ1.034、L.kentuckyensis LDDC 2876-05、L.violacea LM 036、L.violacea CAS 5-124、L.violacea 173536、L.waywayandensis LL-37Z-15、L.waywayandensis 173919、L.waywayandensis 173629.
The bacterium group of formula 34 be divided into L.albida NA 944235, the 1 of L.albida R10069:The mixture of 1 (wt)；
Bacterium group in formula 35-39 is divided into single bacteria component, contains respectively：S.ginsenosidimutans BXN5-15、S.pauli B33D1、S.phytolaccae GTGR-8、S.soli Gsoil 355、S.taibaiensis GTJR-20.
The bacterium group of formula 40 be divided into S.ginsenosidimutans BXN5-15, the 1 of S.pauli B33D1:The mixture of 1 (wt)；
Bacterium group in formula 41-46 is divided into single bacteria component, contains respectively：T.aquatica RIB1-6、T.arctica R9-86、T.ferruginea 3576、T.lutea DY、T.pekingensis QH、T.rubra M-8.
The bacterium group that formula 47 is be divided into T.aquatica RIB1-6, the 1 of T.arctica R9-86:The mixture of 1 (wt).
Described additive is silicon dioxide, Kaolin, the mixture of powdered rice hulls, wheat husk powder and powder of straw.
Described binding agent is bentonite and water-soluble vitamins polysaccharide, and weight ratio is for 1:1.
Described protective agent is glycerol, defatted milk, the mixture of vegetable oil, sodium alginate and shitosan.
The impact that the lower five kinds of plant growth-promoting rhizobacterias of embodiment 3 laboratory condition are sprouted to millet
Experimental technique：
3.1 seed treatment
With 1% sodium hypochlorite, surface sterilization is carried out 5 minutes to Millet Seed, and rinsed 5 times with sterile distilled water.Wrap kind of a clothing by the use of 1% carboxymethyl cellulose as sticker, (bacterial content is 10 to plant clothing composition respectively yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), the red rod bacterium (Solirubrobacter) of soil, autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof and peat (Biocare Technology Pvt., Australia) mixture8-109CFU/g peat), it is placed in air drying afterwards.Every seed contains bacterium 106-107CFU.Comparison seed is wrapped up with the peat without bacterium.
3.2 in vitro growth-promoting tests
The seed that bacterial treatment is crossed and comparison seed are seeded on napkin.Napkin is immersed in distilled water, 50 seeds equably placed by every napkin.Then covered above with another wet paper towel, so that seed is fixed.Napkin " sandwich " is rolled, is placed in valve bag and prevents drying.After 7 days, take out napkin, the number of number chitting piece after expansion.Simultaneously, using Abdul Baki and Anderson (1973) (Abdul-Baki, A.A., ＆Anderson, J.D. (1973) .Vigor determination in soybean seed by multiple criteria.Crop science, 13 (6), 630-633.) the method analysis seedling vigor that describes, measure the root length of each seedling and bud length to calculate seedling vigor index (vigor index), computing formula is as follows：
Vitality index=(average root length+average bud length) × germination percentage (%)
Each processes 4 Duplicate Samples, and each Duplicate Samples is with 100 seeds.Three repetitions, carry out multiple check (p using Tukey<0.05).
Experimental result：
The facilitation that under table 7 laboratory condition, yellow soil source bacterium sprouts to millet
Note：Data is meansigma methodss ± SD；Different letter representation significant difference (p after every column of figure<0.05), table below is identical with this.
The facilitation that under table 8 laboratory condition, Ferruginibacter bacterium sprouts to millet
The facilitation that under table 9 laboratory condition, Lenze Salmonella sprouts to millet
The facilitation that under table 10 laboratory condition, soil redness rod bacterium sprouts to millet
The facilitation that under table 11 laboratory condition, autochthonal Zymomonas mobiliss are sprouted to millet
The facilitation that under table 12 laboratory condition, strain combinations are sprouted to millet
Result is as shown in table 7-12 and Fig. 1-6.Result shows, process Millet Seed through yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof respectively, seed can be promoted to sprout with distinct program, and seedling bud length can be dramatically increased and root is long, germination percentage also substantially increases.And, all yellow soil sources bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof promote Millet Seed sprouting effect to be superior to known Promoting bacteria INR7, and seedling vigor index is obviously higher than INR7 and blank.
The growth-promoting functions to millet for the lower five kinds of plant growth-promoting rhizobacterias of embodiment 4 condition of pot
Experimental technique：
Select unpasteurized field soil as potting media, put in the basin alms bowl of a diameter of 15cm.10 seeds sowed by every basin, and after germinateing one week, every basin retains 5 plants, makes a call to three holes, water about 30mL bacteria suspension respectively, filled out hole with soil near every plant of seedling.The seedling watered with clear water is as comparison.By basin alms bowl as in greenhouse, 22～32 DEG C of temperature control (26 DEG C of mean temperature).Watered once with 20ml sterile distilled water every 2 days, moisturizing by weight is to reach basin bowl-entering soil earth water capacity weekly.
After plant strain growth 30 days, measurement aerial partss height (plant height), leaf area (LI-3100C Area Meter respectively, LI-COR, Inc., USA), root length (WinRHIZO, Regent Instruments Inc., USA), the index such as plant fresh weight and plant weights (drying to constant weight for 70 DEG C).
According to randomized complete-block design, each processes 3 Duplicate Samples, 2 repetitions to all greenhouse experiments.
Experimental result：
Under table 13 condition of pot, yellow soil source bacterium is to millet growth-promoting functions
Under table 14 condition of pot, Ferruginibacter bacterium is to millet growth-promoting functions
Under table 15 condition of pot, Lenze Salmonella is to millet growth-promoting functions
Under table 16 condition of pot, soil redness rod bacterium is to millet growth-promoting functions
Under table 17 condition of pot, autochthonal Zymomonas mobiliss are to millet growth-promoting functions
Under table 18 condition of pot, strain combinations are to millet growth-promoting functions
Result is as shown in table 13-18 and Fig. 7-12.Result shows, under condition of pot, yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof can increase millet plant plant height, leaf area, beetroot yield, tiller number, fresh weight and dry weight respectively to some extent, growth-promoting functions are fairly obvious, and effect is better than known Promoting bacteria INR7.
The growth-promoting functions to Semen Maydiss for the lower five kinds of plant growth-promoting rhizobacterias of embodiment 5 condition of pot
Experimental technique：
Corn seed sterile distilled water dries after repeatedly cleaning, and is soaked in 0.1% HgCl210min in solution, is rinsed with sterile distilled water immediately and dries for more than 10 times, and checks whether sterilization is thorough.
After corn seed surface sterilization, 45 DEG C of water bath with thermostatic control 4h.Microbial inoculum is placed in larger basin, plus proper amount of clear water melts, and is tuned into pasty state, put into seed and stir, allow every seed be stained with microbial inoculum.Positive control is acted on growth promoting bacteria agent on the market green Feng Kang seed dressing, to be inoculated as negative control with clear water.Seed after processing is wrapped in sterile gauze, sprays 3 sterilized water, accelerating germination 3d under room temperature condition daily.The corn seed of germination is transplanted in basin alms bowl, about 10, every basin, depth about 1.5cm, and each basin alms bowl is placed in room temperature and cultivates under natural lighting.Daily sterile distilled water sprays 2 times.The various Morphologic Parameters of each test group and matched group milpa are measured, including plant height, root length, fresh weight and dry weight after processing 33d.
Wherein, the commercially available growth promoting bacteria agent as positive control, the information of such as green Feng Kang 988 composite bacteria agent capable (embodiment 5-6) and green section microbial manure (embodiment 8) is as shown in table 5.
Table 19 is used as the commercially available growth promoting bacteria agent information of positive control
Experimental result：
Under table 20 condition of pot, yellow soil source bacterium is to Semen Maydiss growth-promoting functions
Under table 21 condition of pot, Ferruginibacter bacterium is to Semen Maydiss growth-promoting functions
Under table 22 condition of pot, Lenze Salmonella is to Semen Maydiss growth-promoting functions
Under table 23 condition of pot, soil redness rod bacterium is to Semen Maydiss growth-promoting functions
Under table 24 condition of pot, autochthonal Zymomonas mobiliss are to Semen Maydiss growth-promoting functions
Under table 25 condition of pot, strain combinations are to Semen Maydiss growth-promoting functions
Result is as shown in table 20-25 and Figure 13-18.Result shows, yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof are obvious to the growth-promoting effect of Semen Maydiss respectively, plant height, root length, fresh weight and dry weight aspect are obviously higher than comparison, and effect is higher than the commercial green Feng Kang of growth promoting bacteria agent.
The growth-promoting functions to Semen Tritici aestivi for the lower five kinds of plant growth-promoting rhizobacterias of embodiment 6 condition of pot
Experimental technique：
Use the sodium chloride solution seed selection of saturation first, select full complete wheat seeds, the hydrogen peroxide surface sterilization 5min being 10% with concentration, rinsed 4-5 time with distilled water.Wheat seed is equably sprinkling upon in seedlings nursing plate, transplants after waiting germination.
During transplanting, microbial inoculum and substrate are tuned into pulpous state, the root of adhesion Semen Tritici aestivi, are transferred in basin alms bowl.Basin pot mouth footpath is 12cm, and basin bottom part puts double-layer filter paper, adds the fluvial sand of sterilization, is highly away from pelvic surface of sacrum 1.5cm.The consistent seedling of 5 plants of growing ways uniformly transplanted by every basin.If 2 comparisons, one is processed with green Feng Kang process, its dual-purpose clear water.Each processes 3 basins, 3 repetitions.
Add sterilized water in basin alms bowl in good time, add 5ml Hoagland nutritional solution it is ensured that suitable moisture and nutrient supply every the every basin of 5d.Plant height, root length, fresh weight and dry weight is measured after 25d.
Experimental result：
Under table 26 condition of pot, yellow soil source bacterium is to Semen Tritici aestivi growth-promoting functions
Under table 27 condition of pot, Ferruginibacter bacterium is to Semen Tritici aestivi growth-promoting functions
Under table 28 condition of pot, Lenze Salmonella is to Semen Tritici aestivi growth-promoting functions
Under table 29 condition of pot, soil redness rod bacterium is to Semen Tritici aestivi growth-promoting functions
Under table 30 condition of pot, autochthonal Zymomonas mobiliss are to Semen Tritici aestivi growth-promoting functions
Under table 31 condition of pot, strain combinations are to Semen Tritici aestivi growth-promoting functions
Result is as shown in table 26-31 and Figure 19-24.Result shows, after processing through yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof respectively, millet plant height, root length, fresh weight and dry weight have different degrees of raising compared with the control, and growth-promoting effect is better than the commercial green Feng Kang of growth promoting bacteria agent.In addition, from wheat growth situation it can also be seen that each process wheat leaf color is pale yellowish green, blade profile is abundant, forms obvious difference with compareing.
The growth-promoting functions to millet for the lower five kinds of plant growth-promoting rhizobacterias of embodiment 7 field condition
Choose a piece of land and evaluate five kinds of plant growth-promoting rhizobacteria impacts to Growth of Millet under field condition, soil is red soil.During site preparation, microbial inoculum is mixed thoroughly with chemical fertilizer (carbamide, Diammonium phosphate (DAP), potash fertilizer, compound fertilizer etc.) or fine sand (sand, soil, plant ash, wood flour etc.), uniformly spreads fertilizer over the fields, with mixing with spreading.Positive control cell adds INR7 microbial inoculum；Negative control cell is not added with microbial inoculum.Each processes 4 cells, and each cell is a line of 5m length, sows about 50～100.Pouring in after planting every 15 days is once.After 21 days, often row retains the seedling of same strain number.After 60 days, measurement plant height, leaf surface amass, fringe head number/every plant, fringe head length and fringe head girth.Measure each cell mass of 1000 kernel during results and calculate total output.
Result is as shown in from table 32-37 and Figure 25-30.
The growth-promoting functions to millet for the yellow soil source bacterium under table 32 field condition
The growth-promoting functions to millet for the Ferruginibacter bacterium under table 33 field condition
The growth-promoting functions to millet for the Lenze Salmonella under table 34 field condition
The soil redness growth-promoting functions to millet for the rod bacterium under table 35 field condition
The growth-promoting functions to millet for the autochthonal Zymomonas mobiliss under table 36 field condition
The growth-promoting functions to millet for the strain combinations under table 37 field condition
Result shows, after processing through yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof respectively, every measurement index of millet have different degrees of raising compared with the control, and growth-promoting effect is better than known Promoting bacteria INR7.Show under field condition, yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof can promote Growth of Millet to develop.
The growth-promoting functions to Fructus Lycopersici esculenti for the lower five kinds of plant growth-promoting rhizobacterias of embodiment 8 field condition
After Fructus Lycopersici esculenti is transplanted, carry out different disposal in pouring.Agrotechnical formulation prepared by the embodiment of the present invention 2 is dissolved in water or directly takes bacterial strain fermentation liquor or bacteria suspension to apply with water punching.
If two matched groups.Matched group one：Green section microbial bacteria fertilizer is dissolved in water, same method is applied with water punching；Matched group two is not added with any material, is directly watered with clear water.Each matched group processes 3 replicated plots, and each plot area is about 24m2(3m × 8m), including 3 ridges, totally 6 row, plants 150 plants of Fructus Lycopersici esculenties.Protection row is set, completely random arranges between cell and cell.
Transplant 30d investigation after processing, each cell randomly selects 15 plants of Fructus Lycopersici esculenties, measurement plant height, root length and fresh weight.After Fructus Lycopersici esculenti starts to pluck, the tomato weight of each cell when record is plucked every time, waits Fructus Lycopersici esculenti all to pluck and finishes, calculate each cell total output.
Under table 38 field condition, yellow soil source bacterium is to Fructus Lycopersici esculenti growth-promoting functions
Under table 39 field condition, Ferruginibacter bacterium is to Fructus Lycopersici esculenti growth-promoting functions
Under table 40 field condition, Lenze Salmonella is to Fructus Lycopersici esculenti growth-promoting functions
Under table 41 field condition, soil redness rod bacterium is to Fructus Lycopersici esculenti growth-promoting functions
Under table 42 field condition, autochthonal Zymomonas mobiliss are to Fructus Lycopersici esculenti growth-promoting functions
Under table 43 field condition, strain combinations are to Fructus Lycopersici esculenti growth-promoting functions
Result is as shown in table 38-43 and Figure 31-36.Result shows, after processing through yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof respectively, Fructus Lycopersici esculenti plant height, root length, fresh weight and yield have different degrees of raising compared with the control, and effect is better than the commercial green section of growth-promoting agent.In addition, from tomato growth situation it can also be seen that each process Fructus Lycopersici esculenti stem is sturdy, leaf color is black green, and growing way is preferable.
The growth-promoting functions to other plant for the 9 five kinds of plant growth-promoting rhizobacterias of embodiment
Respectively to other plant, apply five kinds of plant growth-promoting rhizobacterias including crop, vegetable, fruit tree, flowers etc., method of application be respectively kind of a clothing, seed dressing, seed soaking, pouring root, glue root, spread manuer in holes, rush apply, spread fertilizer over the fields, drip irrigation etc..Measurement plant products or plant height respectively, and calculate and apply the rate of growth of plant or rate of increase after yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof respectively.
Result is as shown in table 44-49.
The growth-promoting functions to other plant for the table 44 yellow soil source bacterium
The growth-promoting functions to other plant for the table 45 Ferruginibacter bacterium
The growth-promoting functions to other plant for the table 46 Lenze Salmonella
The table 47 soil redness growth-promoting functions to other plant for the rod bacterium
The growth-promoting functions to other plant for the autochthonal Zymomonas mobiliss of table 48
The growth-promoting functions to other plant for table 49 strain combinations
Result shows, yellow soil source bacterium, Ferruginibacter bacterium, Lenze Salmonella, soil redness rod bacterium, autochthonal Zymomonas mobiliss or a combination thereof can promote different growth and development of plants respectively to some extent, show that yield increases or plant height increases, rate of growth or growth-promoting rate are between 5-50%.
The all documents referring in the present invention are all incorporated as reference in this application, are individually recited as with reference to like that just as each document.In addition, it is to be understood that after the above-mentioned teachings having read the present invention, those skilled in the art can make various changes or modifications to the present invention, these equivalent form of values equally fall within the application appended claims limited range.
Claims (10)
1. a kind of purposes of plant growth-promoting rhizobacteria is it is characterised in that be used for preparing Agrotechnical formulation, described Agrotechnical formulation
For improving the economical character of plant, wherein said plant growth-promoting rhizobacteria is selected from the group：Yellow soil source bacterium
(Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella (Lentzea), soil redness rod bacterium
(Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or a combination thereof.
2. purposes as claimed in claim 1 is it is characterised in that described plant growth-promoting rhizobacteria includes yellow soil source
Bacterium (Flavisolibacter).
3. purposes as claimed in claim 2 is it is characterised in that described yellow soil source bacterium (Flavisolibacter)
It is selected from the group：Radix Ginseng khaki soil source bacterium (Flavisolibacter ginsengisoli), Radix Ginseng ground yellow soil source
Bacterium (Flavisolibacter ginsengiterrae), Flavisolibacter rigui, Flavisolibacter
Sediminifilum or a combination thereof.
4. purposes as claimed in claim 2 is it is characterised in that described yellow soil source bacterium (Flavisolibacter)
It is selected from the group：F.ginsengisoli GS643、F.ginsengiterrae GS492、F.rigui 02SUJ3、
F.sediminifilum HU1-JC5 or a combination thereof.
5. purposes as claimed in claim 1 is it is characterised in that the described economical character improving plant includes：
I () promotes seed to sprout；(ii) improve plant height；(iii) promote root system development；(iv) increase leaf area；V () increases tiller
Number；(vi) improve yield.
6. a kind of Agrotechnical formulation for improving plant agronomic character is it is characterised in that described Agrotechnical formulation includes
(a) plant growth-promoting rhizobacteria of safe and effective amount；(b) auxiliary agent or agronomically acceptable carrier；Wherein, described rhizosphere
Promoting bacteria is selected from the group：Yellow soil source bacterium (Flavisolibacter), Ferruginibacter bacterium, Lenze Salmonella
(Lentzea), soil redness rod bacterium (Solirubrobacter), autochthonal Zymomonas mobiliss (Terrimonas) or its group
Close.
7. Agrotechnical formulation as claimed in claim 6 is it is characterised in that described Agrotechnical formulation contains
1×10-1×1020Cfu/mL or 1 × 10-1 × 1020Cfu/g plant growth-promoting rhizobacteria, preferably 1 × 104-1×1015cfu/mL
Or cfu/g plant growth-promoting rhizobacteria, by cumulative volume or the gross weight meter of described Agrotechnical formulation.
8. Agrotechnical formulation as claimed in claim 6 is it is characterised in that the dosage form of described Agrotechnical formulation is selected from down
Group：Wettable powder, water dispersible granules, suspending agent, aqueous emulsion, granule, seed coat agent or a combination thereof.
9. a kind of method promoting growth and development of plants is it is characterised in that methods described includes step：In plant
Root applies the Agrotechnical formulation described in claim 6.
10. a kind of preparation method of Agrotechnical formulation described in claim 6 is it is characterised in that methods described includes step：
(a) plant growth-promoting rhizobacteria is mixed with (b) auxiliary agent or agronomically acceptable carrier, thus being formed described in claim 6
Agrotechnical formulation.
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| CN112661808A (en) * | 2020-12-23 | 2021-04-16 | 浙江大学 | Depsipeptide compound and preparation method and application thereof |
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| CN110199735A (en) * | 2019-07-08 | 2019-09-06 | 厦门梓蔓生物科技有限公司 | A kind of industrial hemp numerous culture apparatus and breeding method fastly |
| CN112514595A (en) * | 2020-12-02 | 2021-03-19 | 无锡市三阳生态农业发展有限公司 | Rhizosphere growth-promoting bacterium seed soaking method for improving germination rate |
| CN112661808A (en) * | 2020-12-23 | 2021-04-16 | 浙江大学 | Depsipeptide compound and preparation method and application thereof |
| CN114540259A (en) * | 2022-04-25 | 2022-05-27 | 华南农业大学 | Dioscorea composita mycorrhizal fungi growth promoter and dioscorea composita agro-optical complementary planting method |
| CN114540259B (en) * | 2022-04-25 | 2022-07-01 | 华南农业大学 | Dioscorea composita mycorrhizal fungi growth promoter and Dioscorea composita farming photo-complementation planting method |
| US11980191B2 (en) | 2022-04-25 | 2024-05-14 | South China Agricultural University | Compound mycorrhizal fungus growth promoting agent and agricultural-photovoltaic complementary planting method for Dioscorea composita |
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| CN106472568B (en) | 2020-05-12 |
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