CN105961377A - Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability - Google Patents
Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability Download PDFInfo
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- CN105961377A CN105961377A CN201610391816.0A CN201610391816A CN105961377A CN 105961377 A CN105961377 A CN 105961377A CN 201610391816 A CN201610391816 A CN 201610391816A CN 105961377 A CN105961377 A CN 105961377A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a castanopsis hystrix pollen collecting and storing method for increasing the germination rate and improving the pollen viability. The method comprises the following steps that when male parent male flowers are in a transitional period of an early blooming period and a full blooming period, male parent male flower inflorescence is collected at 9:00-11:00 or 15:00-17:00; the male flower inflorescence is dried for 12-24 h in a sterile room at the temperature of 22 DEG C-28 DEG C under illumination of 80 W-160 W, when a large amount of anther blows out and a large amount of yellow pollen scatters during gentle shaking, drying is completed; the dried male flower inflorescence is screened with a pollen sieve, pollen is collected and contained in a container, an opening of the container is sealed, and the container is placed in an environment at the temperature of minue 60 DEG C-minus 100 DEG C or liquid nitrogen to be stored. Furthermore, when the pollen needs to be germinated, the stored pollen is placed in a liquid culture medium and can be germinated at the temperature of 25 DEG C-35 DEG C. Accordingly, a castanopsis hystrix pollen collecting, drying, storing and viability detecting system is formed for the first time, the method is simple, rapid and high in operability, the castanopsis hystrix pollen viability can be improved, and the manual cross pollination success rate of castanopsis hystrix can be increased.
Description
Technical field
The invention belongs to forest genetics technical field.Germination rate can be improved more particularly, to one and extend pollen
The red cone pollen collecting store method of vigor.
Background technology
Red cone (Castanopsis hystrix A. DC) have another name called Hong Li, Chi Li, Li Mu, thorn evergreen chinquapin, red evergreen chinquapin, red minor official evergreen chinquapin,
Red Ke, belongs to Fagaceae Castanopsis aiphyllium, and tree body is logical straight, and the height of tree is up to 30 meters, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches more than 1 meter, and it is China's South Asia heat
Carry and the advantage composition seeds of subtropical vegetation evergreen broadleaf forest, belong to the important broad-leaved preciousness material of one's native land in South China with efficient
Multipurpose ecological public welfare forests seeds.Red cone natural distributed is in east longitude 95o20 '～118o0 ', north latitude 18o30 '～25o0 ', main
The place of production concentrates on Guangdong, Guangxi, south Fujian, and its circumferential distribution is in south of Yunnan, Dong people and Hunan, Jiangxi, good fortune
The south that build, Hainan etc. saves, the Motuo County that marginal distribution darcy is hidden.Red cone has that growth is fast, material is excellent, adaptation is wide, high efficiency
Deng good characteristic.Its trunk is logical straight, material is hard, take on a red color, corrosion resistance by force, do not ftracture, indeformable, be easily worked, be excellent
Matter preciousness material, is available for the use such as building, shipbuilding, high-grade furniture, floorboards of wood, military project articles for use, sports equipment.Seed is rich in shallow lake
Powder, can stir-fry and eat, feedstuff and wine brewing, plants real, acorn-cup all rich in tannin, can obtain through refining tannic extract.Red cone is planted as South China's zonality
By the important component part of Climax community, both there are good ecologic stability and excellent ecological benefits, be again the most excellent
Big footpath level commerical tree species.It has vigorous trophophase and a good speed of growth, once afforestation can fell 10 times with
On, to manage century-old lasting, and be thick with leaves, mixed natural disposition can be good, can carry out pure forest plantation as with material and forests for water supply conservation,
Also can be that inferior woods is transformed, the Mixed forestation of ecological public welfare forests transformation carries out being applicable.Therefore, these seeds have become ground, south China
The highly important Native Broad-leaved Tree Species in district, transforms and pure with forests for water supply conservation, the poor efficiency ecological public welfare forests of red cone construction every year
Woods area is more than 3500 hectares, at present, in Guangdong, Guangxi, the provinces and regions such as Fujian built various artificial forest more than 30,000 hectare.Become
One of important reproducting tree species for south China area.
But, the overall improvement degree of red cone is the highest, and improvement system is the most perfect, breeding supply critical shortage, it has also become
Restrict the bottleneck that red cone artificial forest develops rapidly.Pass through cross-breeding, it is achieved the preferable restructuring of excellent effective gene, all the time
It it is the approach most effective, the quickest, safest of rearing new variety.Particularly Forest Tree New Varieties, the forest of the most about more than 80%
New varieties rely on cross-breeding and obtain.But, forest is of a great variety and varies, and causes forest crossbreeding technology also multiple
Miscellaneous various, it is a great problem for the breeding objective completing red cone the most in high quality；Comparing other seeds, the hybridization of red vertebra is more
For complexity, reason is: the inflorescence of red cone is complex, is made up of pure staminate inflorescence, pure female inflorescence and both sexes inflorescence, and pollen amount is relatively
Greatly, pollen grain is small and light, and the caking that easily makes moist, and female chapiter is the typical moist easy pollination failures of stigma spontaneous pollination,
Cause empty shell rate high.
Therefore, florescence artificial supplementary pollination promotes percentage of fertile fruit, improves seed production, is more effective solution.Flower
Powder gather, be dried, storage, vitality test technology, be to realize the necessary sport technique segment of this measure.Pollen is phanerogamous hero
Gametocyte, plays an important role in sexual propagation.The sprouting of pollen, pollen tube growth are the important of sexual plant reproduction
Link, comprises many complicated physiological process.Pollen completes fertilization process with pollen tube for passage conveying sperm, the sprouting of pollen
It is to realize amphigameous key with pollen tube growth.
The pollen grain of red cone is small and light, and the caking that easily makes moist, and therefore, the different breeding parent of the red cone of systematic study is by environment
The biological features of flowering of impact, develops effective pollen collection, dry, Techniques of preserving, emasculation isolation technology and efficiently pollinates
Promote fruit development technology, form a set of efficient hybrid artificialpollination technology, criticize realizing superior hybrid crosses new varieties
Quantify production to have great importance.
Summary of the invention
The technical problem to be solved in the present invention is defect and the technical deficiency overcoming above-mentioned prior art, it is provided that a kind of red cone
Pollen collection, dry, preservation and viability examination technology, it is possible to increase red cone pollen germination rate and prolongation Pollen Activity.
It is an object of the invention to provide a kind of red cone pollen collecting preservation that can improve germination rate and extend Pollen Activity
Method.
The present invention another object is that the above-mentioned red cone pollen collecting preservation side can improved germination rate and extend Pollen Activity
The application of method.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of red cone pollen collecting store method that can improve germination rate and extend Pollen Activity, comprises the steps:
S1. pollen collection: when male parent male flower is in initial bloom stage with the full-bloom stage transition period, in 9:00-11:00 or 15:00-17:
00 gathers male parent staminate inflorescence, loads in sulphuric acid paper bag；
S2. pollen is dried: the staminate inflorescence of collection is dried under 80～160W illumination in 22～28 DEG C of sterilizing roomes 12～24h and does
Dry, notice time dry that pollen is isolated, and prevents cross-contamination；When flower pesticide bursts in a large number, shake has a large amount of yellow pollen to be scattered gently
Time；
S3. pollen storage: dried staminate inflorescence crosses pollen sieve, removes the removal of impurity and filigree, collects pollen, loads in container, envelope
Mouthful, it is placed in-60 DEG C～-100 DEG C or liquid nitrogen and preserves.
Further, when needs pollen germination, the pollen of storage is placed in fluid medium, sprouts at 25 DEG C～35 DEG C
Send out.
Preferably, described sprouting is to cultivate 24h under 35 DEG C of constant temperature to sprout.
Preferably, consisting of of described fluid medium: 50～200g/L sucrose, 500～1000 mg/L boric acid, 5～
10g/L agar, surplus is water.
It is highly preferred that the consisting of of described fluid medium: 200g/L sucrose, 1000 mg/L boric acid, 8g/L agar, remaining
Amount is water.
Furthermore it is preferred that initial bloom stage described in above-mentioned steps S1 and full-bloom stage transition period are when male parent male flower 50～75% flower
Silk stretches out, and when being faint yellow；Or for stretching out when male parent male flower 100% filigree, and when being yellow.
It is highly preferred that initial bloom stage described in step S1 and full-bloom stage transition period are when male parent male flower 50～75% filigree stretch out,
And when being faint yellow.
Preferably, gathering described in step S1 is to gather, in 9:00-11:00 or 15:00-17:00, the fresh male flower opened the same day
Sequence.
It is highly preferred that gathering described in step S1 is to gather, in 10:00 or 16:00, the fresh staminate inflorescence opened the same day.
Preferably, being dried described in step S2 is to be dried 18～24h in 25 DEG C of sterilizing roomes under 100W illumination.
Preferably, preserve during described in step S3, storage is placed in-80 DEG C or liquid nitrogen.
Preferably, described in step S3, the mesh number of pollen sieve is 90～110 mesh.
It is highly preferred that the mesh number of pollen sieve is 100 mesh described in step S3.
Preferably, the method sieved described in step S3 is to be poured into by dried staminate inflorescence in pollen sieve, while light with brush pen
Light stone roller moves inflorescence, and limit is shaken gently, sieves.
Preferably, container described in step S3 is vial.
Preferably, described in step S3, sealing is to seal with preservative film.
It addition, said method is in terms of improving red cone pollen germination rate and/or extending Pollen Activity, and in red cone hybridization
Application in terms of breeding, all should be within protection scope of the present invention.
The method have the advantages that
1, the present invention red cone pollen drying means, is dried 12～24h under 4000Lx daylight lamp, dries in the shade compared to conventional indoor
Or be dried in exsiccator, rapid draing can the most not destroy pollen activity, the most also can be observed to brush again when flower pesticide bursts completely
Powder sieves, and solves red cone pollen grain small and light, not this difficult problem of easily collecting.
2, the present invention red cone pollen store method, be individually positioned in room temperature, 4 DEG C ,-20 DEG C ,-80 DEG C, liquid nitrogen etc. different
Environment is preserved, is detected by Pollen Activity, detect along with the holding time constantly increases, the Pollen Activity preserved in liquid nitrogen
The strongest, next to that-80 DEG C, significantly improve red cone pollen storage rate.
3, this method use fluid medium in containing 0.8% agar, 20% sucrose, 1000ppm boric acid preparation and
Becoming, liquid stabilising is reliable, is suitable for red cone pollen and leaves one's post cultivation.
4, by dried red cone pollen, by wrist strength, shaking on solid medium, pollen can be straight with oxygen
Contact, and pollen is evenly distributed, pollen is cultivated 24h under 35 DEG C of constant temperature and can be sprouted.
5, this method defines red cone pollen collection, dry, preservation and the system of viability examination first, and method has letter
The feature such as single, rapid and workable, can improve the success rate of red cone artificial hybridization pollination, can effectively solve red cone breeding garden
The problem of solid biennial bearing, improves yield and the quality of breeding garden, enables breeding garden effectively to play its due function.
Accompanying drawing explanation
Fig. 1 is 5 kinds of different flowering state staminate inflorescence.
Fig. 2 is Duncan multiple comparisons figure (α=0.01) of different staminate inflorescence state.
Fig. 3 is the multiple comparisons figure (α=0.01) of red cone Pollen Activity acquisition time.
Fig. 4 is different drying modes red cone Pollen Activity multiple comparisons figure (α=0.01).
Fig. 5 is that 5 kinds of preserving type differences preserve natural law red cone Pollen Activity.
Fig. 6 is the red cone pollen cultures pollen germination testing result after 24 hours collecting the same day.
Fig. 7 is the red cone pollen that the inventive method is collected, is dried, encapsulates.
Detailed description of the invention
Further illustrate the present invention below in conjunction with Figure of description and specific embodiment, but embodiment is not to the present invention
Limit in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus are the examination of the art routine
Agent, method and apparatus.
Unless stated otherwise, agents useful for same of the present invention and material are commercial.
Embodiment 1 gathers the research on opportunity
1, under following five kinds of staminate inflorescence states, red cone staminate inflorescence is gathered respectively:
(1) state 1(filigree just stretches out)；
(2) state 2(filigree stretches out 20～30%, faint yellow)；
(3) state 3(filigree stretches out 50～75%, faint yellow)；
(4) state 4(filigree stretches out 100%, yellow)；
(5) state 5(filigree stretches out 100%, brown).
Different flowering state staminate inflorescence is as shown in Figure 1.
2, the staminate inflorescence gathered is indoor in the aseptic culture of 25 DEG C of room temperatures, after being dried 24h under 4000Lx daylight lamp, by pollen
Shake, to (0.8% agar+20% sucrose+1000ppm) on solid medium, is cultivated 24h, is used optical microscope under 35 DEG C of constant temperature
Observe the sprouting number of pollen, pollen tube length, detect Pollen Activity.
3, result is as shown in table 1, and state 3(filigree stretches out 50～75%, faint yellow) time the staminate inflorescence that gathers, through processing
After Pollen Activity the highest (74.07%), state 4(filigree stretches out 100%, yellow) take second place, Pollen Activity is 65.59%.
The red cone Pollen Activity table of the different staminate inflorescence state acquisition of table 1
2, using R-3.2.1 software that the Pollen Activity under 5 kinds of states of staminate inflorescence is carried out variance analysis, result is as shown in table 2, no
With staminate inflorescence state, Pollen Activity being had pole appreciable impact, F value is 54.3, and p value is 4.20e-09, shows different staminate inflorescence shape
Pole significant difference is there is in state collecting sample to Pollen Activity.
Further Duncan multiple comparisons figure (accompanying drawing 2) display, on significant level α=0.01, state 3(filigree stretches out 50
～75%, faint yellow) and state 4(filigree stretch out 100%, yellow) be noticeably greater than other states.
The analysis of variance table of table 2 red cone Pollen Activity staminate inflorescence state
Note: significant level, * * *: 0.001.
The research of embodiment 2 acquisition time
1, from 8:00～18:00 in morning, gather a pollen every 1h, add up to 11 times.
2, the aseptic culture in 25 DEG C of room temperatures is indoor, after being dried 24h, pollen is shaken solid training under 4000Lx daylight lamp
Support on base (0.8% agar+20% sucrose+1000ppm), cultivate 24h under 35 DEG C of constant temperature, with sprouting of observation by light microscope pollen
Send out number, pollen tube length, detect Pollen Activity.
3, result is as shown in table 3: pollen germination has two peak values, is 10:00 and 16:00 respectively.Time, too early dew was not
Dispersing, pollen is easily adhered, and is unfavorable for loose powder, and pollen germination rate is low.The meridian hour, the sun is exposed to the sun, and temperature is higher, is also unfavorable for flower
Powder is sprouted.Therefore selecting the pollen collection time is 9:00～11:00,15:00～17:00.
The red cone Pollen Activity of table 3 different acquisition time measures table
4, using R-3.2.1 software that the Pollen Activity under the different acquisition time is carried out variance analysis, result is as shown in table 4, no
Gathering pollen with acquisition time, there is pole significant difference between Pollen Activity, F value is 28.63, and p value is 3.80e-15.
The analysis of variance table of table 4 different acquisition time red cone Pollen Activity
Further Duncan multiple comparisons figure (accompanying drawing 3) display, on significant level α=0.01, time point 3(10:00) and the time
Point 9(16:00) Pollen Activity is the highest, noticeably greater than other times point.
The research of embodiment 3 drying means
1, the staminate inflorescence of collection is carried out following 9 kinds of distinct methods be dried:
(1) natural drying 24h；
(2) 60 degree of 24h of baking oven；
(3) 100w is dried 6h；
(4) 100w is dried 9h；
(5) 100w is dried 12h；
(6) 100w is dried 18h；
(7) 100w is dried 24h；
(8) 100w is dried 48h；
(9) 100w is dried 72h.
Then carry out Pollen Activity detection, repeat for 5 times.
2, result shows: the method that 100W daylight lamp is dried generally is substantially better than natural drying and baking oven 60 be dried wait normal
Rule method.And 100W daylight lamp is dried 12～24h, best results.Time is too short, and pollen water content is high, causes pollen germination rate；
Low overlong time, kills Pollen Activity, also reduces germination rate.
The different drying mode red cone Pollen Activity of table 5 measures table
3, the red cone Pollen Activity after using R-3.2.1 software to process different drying modes carries out variance analysis, result such as table 6
Shown in, F value is 26.81, and p value is 4.30e-12, uses different drying mode to process, and Pollen Activity exists pole significance difference
Different.
The analysis of variance table of table 6 red cone Pollen Activity drying mode
Note: significant level, * * *: 0.001.
Further Duncan multiple comparisons figure (accompanying drawing 4) display, on significant level α=0.01, drying mode 2,6,7,8,
Significant difference between 9, drying mode most preferably 7(100w is dried 24h).
The research of embodiment 4 store method
1, dried pollen is loaded in vial, seals, be respectively placed under following several different Conservation environment and preserve:
(1) room temperature；
(2) 4 DEG C；
(3)-20 DEG C；
(4)-80 DEG C；
(5) liquid nitrogen.
When respectively preserving 0d, 5d, 10d, 20d, 30d, 60d respectively, carry out Pollen Activity detection, repeat for 5 times.
2, result is as shown in table 7 and accompanying drawing 5, and after preserving 5d, decline in various degree, room temperature preservation all occurs in Pollen Activity
Under Pollen Activity decline the fastest, the Pollen Activity preserved in liquid nitrogen almost without change ,-80 DEG C decline the slowest.Preserve 30d
After, the Pollen Activity of room temperature preservation completely loses, and 4 DEG C ,-20 DEG C of pollen preserved, Pollen Activity reduces 50%, and liquid nitrogen is protected
The Pollen Activity change deposited is less.
The red cone Pollen Activity of the different preserving type of table 7 measures table
3, use R-3.2.1 software that the Pollen Activities preserving natural law mensuration different under different preserving types are carried out variance analysis,
Result is as shown in table 8, has pole significant difference between store method and holding time to Pollen Activity.Along with prolonging of holding time
Long, different preserving types also show notable difference, and preserving type 5 is substantially better than other modes, next to that preserving type 4,
Preserving type 1 effect is worst.
The analysis of variance table of table 8 red cone Pollen Activity preserving type
Note: significant level, * * *: 0.001.
Embodiment 5 red cone pollen collection, the method being dried, preserving
1, a kind of red cone pollen collecting store method that can improve germination rate and extend Pollen Activity, comprises the steps:
S1. pollen collection: when male parent male flower 50～75% filigree stretch out, and when being faint yellow, gathers the same day in 10:00 or 16:00
The fresh staminate inflorescence opened, loads in sulphuric acid paper bag；
S2. pollen is dried: the staminate inflorescence gathered is dried under 100W illumination in 25 DEG C of sterilizing roomes 18～24h, notes time dry
Pollen is isolated, and prevents cross-contamination；When flower pesticide bursts in a large number, when shake has a large amount of yellow pollen to be scattered gently；
S3. pollen storage: being poured into by dried staminate inflorescence in 100 mesh pollen sieves, while grind inflorescence gently with brush pen, limit is gently
Shake, sieves, and removes the removal of impurity and filigree, collects pollen, loads in vial, and preservative film seals, and is placed in liquid nitrogen storage.
The most further, when needs pollen germination, the pollen of storage is placed in fluid medium, under 35 DEG C of constant temperature
Cultivate 24h to sprout；Consisting of of described fluid medium: 200g/L sucrose, 1000 mg/L boric acid, 8g/L agar, surplus is
Water.
2, the red cone pollen cultures that above-mentioned collection was opened the same day 24 hours, detect pollen germination, and result is as shown in Figure 6.
3, the red cone pollen collect through said method, be dried, encapsulated is as shown in Figure 7.
It addition, the pollen collected through said method, be dried, encapsulate, preserve, the holding time is Pollen Activity when 60d
Not yet there is notable change.
Claims (10)
1. one kind can be improved germination rate and extend the red cone pollen collecting store method of Pollen Activity, it is characterised in that include
Following steps:
S1. pollen collection: when male parent male flower is in initial bloom stage with the full-bloom stage transition period, in 9:00-11:00 or 15:00-17:
00 gathers male parent staminate inflorescence；
S2. pollen is dried: the staminate inflorescence of collection is dried under 80～160W illumination in 22～28 DEG C of sterilizing roomes 12～24h and does
Dry；When flower pesticide bursts in a large number, when shake has a large amount of yellow pollen to be scattered gently；
S3. pollen storage: dried staminate inflorescence crosses pollen sieve, collects pollen, loads in container, and sealing is placed in-60～-100
DEG C or liquid nitrogen in preserve.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, when pollen need to be sprouted, the pollen of storage is placed in fluid medium, sprout at 25～35 DEG C.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, initial bloom stage described in step S1 and full-bloom stage transition period are when male parent male flower 50～75% filigree stretch out, and are light
During yellow；Or for stretching out when male parent male flower 100% filigree, and when being yellow.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, gathering described in step S1 is to gather, in 9:00-11:00 or 15:00-17:00, the fresh staminate inflorescence opened the same day.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, being dried described in step S2 is to be dried 18～24h in 25 DEG C of sterilizing roomes under 100W illumination.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, preserve during storage is placed in-80 DEG C or liquid nitrogen described in step S3.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, described in step S3, the mesh number of pollen sieve is 90～110 mesh.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, the method sieved described in step S3 is to be poured into by dried staminate inflorescence in pollen sieve, while grind gently with brush pen
Dynamic inflorescence, limit is shaken gently, sieves.
The red cone pollen collecting store method that can improve germination rate and extend Pollen Activity the most according to claim 1,
It is characterized in that, container described in step S3 is vial, and described sealing is to seal with preservative film.
10. method described in claim 1 is in terms of improving red cone pollen germination rate and/or extending Pollen Activity or in red cone hybridization
Application in terms of breeding.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106689121A (en) * | 2016-11-23 | 2017-05-24 | 东北林业大学 | Ultralow-temperature preservation method for pollen of Euonymus bungeanus |
| CN112400484A (en) * | 2020-11-27 | 2021-02-26 | 广西壮族自治区农业科学院 | Cassava pollen collecting and storing method |
| CN113465298A (en) * | 2021-07-16 | 2021-10-01 | 四川农业大学 | South Asia loquat pollen drying and storing method |
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| CN104351170A (en) * | 2014-09-23 | 2015-02-18 | 江苏省林业科学研究院 | Shrub willow pollen preservation method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689121A (en) * | 2016-11-23 | 2017-05-24 | 东北林业大学 | Ultralow-temperature preservation method for pollen of Euonymus bungeanus |
| CN112400484A (en) * | 2020-11-27 | 2021-02-26 | 广西壮族自治区农业科学院 | Cassava pollen collecting and storing method |
| CN113465298A (en) * | 2021-07-16 | 2021-10-01 | 四川农业大学 | South Asia loquat pollen drying and storing method |
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