CN106613895A - Cultivation method of tomatoes of which leaves capable of producing astaxanthin - Google Patents
Cultivation method of tomatoes of which leaves capable of producing astaxanthin Download PDFInfo
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- CN106613895A CN106613895A CN201610003570.5A CN201610003570A CN106613895A CN 106613895 A CN106613895 A CN 106613895A CN 201610003570 A CN201610003570 A CN 201610003570A CN 106613895 A CN106613895 A CN 106613895A
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- fruit
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- astaxanthin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a cultivation method of a new tomato variety Yun shrimp No.3. The new tomato variety Yun shrimp No.3 is obtained by taking Yun hybrid No.8 which is hybridized by taking 92-23-7A as a female parent and 94-5-12F as a male parent as a female parent and astaxanthin-producing engineering tomatoes Shrimp red No.1 as a male parent for hybridization, the astaxanthin in leaves can be extracted by using acetone, and the content of the astaxanthin can be detected by using a UPLC (Ultra Performance Liquid Chromatography) method. The Yun shrimp No.3 is combined with main excellent characteristics of the female and male parents, the disease resistance is strong, the fruit quality is good, and the astaxanthin yield is high. The new tomato variety Yun shrimp No. 3 has the main characteristics that the plant is in determinate growth, the plant height is about 130 cm, the plant is dark red, the growth is healthy and strong, and the disease resistance is strong; the stem is yellow green to light brown, the branching ability is very strong, and the leaf yield is high; the leaf shape is common, the leafstalk is yellow green to dark red, the leaves are dark red, and the average content of a dry matter-astaxanthin is up to 2.64 mg/g; the sepal is green to dark red, the radial corolla is orange-yellow to orange-red, the anther is orange to red, and the stigma and the survival young fruits are brownish red; the fruits are bright red, the neak is purple red and is round, the fruit shape index is 0.8 to 0.92, the fruit surface is smooth, the glossness is bright, the sarcocarp is thick, the number of carpels is 5 to 6, the hardness is large, the average single fruit weight is 180 g, and the maximum single fruit weight is 400 g.
Description
Technical field：
The invention belongs to biological technical field, and in particular to the breeding method of tomato plants Hybrid, more specifically, relating to
And with ' cloud is miscellaneous No. 8 ' for being obtained for paternal hybrid as maternal, ' 94-5-12F ' by ' 92-23-7A ' for female parent, to produce astaxanthin work
Journey tomato ' shrimp is red No. 1 ' obtains that fruit quality is good for paternal hybrid, and disease resistance is strong, the tomato triple hybrid of blade high-yield astaxanthin
The breeding method of ' cloud shrimp No. 3 '.
Background technology：
The female parent ' 92-23-7A ' and male parent ' 94-5-12F ' of ' cloud is miscellaneous No. 8 ' is by Yunnan Prov Agriculture University's gardens gardening
The self-mating system of the autonomous seed selection of tomato seminar of institute.Maternal ' 92-23-7A ' fruit quality is excellent, and fruit type is good, individual big.Male parent
' 94-5-12F ' has high disease resistance.
' cloud is miscellaneous No. 8 '：Determinate growth, 130～150cm of plant height, growth is strong, and disease resistance is strong, belongs to Early-medium maturing variety；Really
Real large red, uniform coloring, high circular, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 4～5, and hardness is big, and storage tolerance is good,
Shelf life is long, average fruit weight 210g, maximum fruit mass up to more than 500g, in Kunming Areas spring greenhouse cultivation, from
Harvesting is seeded into for 180 days or so；Yuanmou area July is seeded into harvesting needs 130 days or so.Outdoor cropping is per 667m2Produce
, up to more than 6000kg, Protectorate cultivation is per 667m for amount2Yield is up to more than 7000kg.Food flavor is good, and sandy taste is sweet, is dish use kind
Eggplant improved seeds, while as processing, filling cultivation.
Produce astaxanthin engineering tomato ' shrimp is red No. 1 '：Its plant, flower, leaf, fruit morphologically with Lycopersicon esculentum indifference, it is main
If the difference in color.Specifically, plant determinate growth, plant height 110cm or so.Stem be half erect type, thick 2.6～
4.7cm, 4～6 branches, yellow green is to light brown.Common blade profile, petiole yellow green to kermesinus, blade kermesinus.Calyx is dark
Redness, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit is subsphaeroidal,
Chinese red after maturation, average fruit weighs about 100g, and the mean value of its fruit content astaxanthin is 1.63mg/g.
Hybridized for male parent by maternal, product astaxanthin engineering tomato ' shrimp is red No. 1 ' of common cultivation tomato ' cloud is miscellaneous No. 8 '
Breeding, so as to obtain, a kind of fruit quality is good, and disease resistance is strong, the breeding method of the tomato variety of blade high-yield astaxanthin and technology
There is not been reported.
The content of the invention：
It is an object of the invention to provide one kind is entered by maternal, product astaxanthin engineering tomato of common cultivation tomato for male parent
A kind of row crossbreeding, fruit quality of acquisition is good, and disease resistance is strong, the breeding method of the tomato variety of blade high-yield astaxanthin.
The above-mentioned purpose of the present invention is realized by techniques below scheme：
The breeding method of tomato variety ' cloud shrimp No. 3 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting,
Seed Treatment, content astaxanthin determination step, the method is obtained for paternal hybrid with ' 92-23-7A ' as maternal, ' 94-5-12F '
' cloud is miscellaneous No. 8 ' for arriving is female parent, and produce astaxanthin engineering tomato ' shrimp is red No. 1 ' carries out hybridization cultivation ' cloud shrimp No. 3 ' for male parent, uses third
Ketone extracts blade astaxanthin, and using UPLC methods its content is detected.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein described pollen collection step is that male parent is more female
This early sowing 20 days, the full-bloom stage morning, collection male parent ' shrimp is red No. 1 ' petal launches 170-190 ° and pattern is partially red after dew is dry
Flower, chooses stamen, spreads out on blank sheet of paper, indicates kind name and collection date, dries in the shade naturally, treats Anther dehiscence, when pollen sheds,
Collect pollen standby；Described maternal emasculation exposes for pollination the previous day selection ' cloud is miscellaneous No. 8 ' corolla elongation, color is by green turn
Yellow, petal is not opened or crack, flower pesticide is in yellow green but shed pollen, the bud bloomed in next day of not ftractureing carries out emasculation；It is described
Artificial pollination be after maternal field dew is dry, to pollinate in fine day morning 8-9 points, 20～25 DEG C of temperature；Described fruit is adopted
Receipts are 50～60 days harvesting ripening fruits after pollination；Described Seed Treatment is after complete red ripe fruit harvesting, after-ripening 1～
Kind is taken again within 2 days；It is to use acetone extraction blade astaxanthin, UPLC methods to detect its content that described content astaxanthin is determined.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein collect in described pollen collection step
Pollen is temporarily stored with 4 DEG C of refrigerator cold-storages, and long-term storage silica gel makees -80 DEG C of Refrigerator stores of drier.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein described maternal emasculation step is steady with left hand
Bud base portion is held, right hand tweezers clamp flower pesticide and all extract its stamen, or together peel off together with petal, emasculation is clean, goes
Isolate after hero, while rejecting the flower for being not suitable for pollinating and selfing fruit.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein described artificial pollination step temperature be 20～
25 DEG C, dip a small amount of pollen point with the rubber tip of common pencil and invest on the column cap of maternal emasculation flower, or directly by pollen
It is placed on paper, the column cap of directly contact emasculation dips a small amount of pollen.With 6 × 8cm tea bag bag baggings, paper clip is fixed, listed to mark
Note, indicates Parent combination number and pollination date on plate, bag taking after pollinating 10 days, the situation while observation is beared fruit, whole in time
Branch cleaning, removes the inflorescence of the bud and new life not pollinated, and training is pruned, and extracts unnecessary axillary bud and the branch of excessive growth, enters water-filling
Fertile coordinated management and the prevention and control of plant diseases, pest control.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein only harvest growth during described fruit harvesting sending out
Normal fruit is educated, unmarked fallen fruits and malformed fruit is not harvested.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein described Seed Treatment step is will with knife
Fruit is laterally cut open, seed and glue flesh is clamp-oned in anhydrous nonmetallic vessel and is fermented 1～2 day, is white to fermentate surface,
Stirred with wooden stick, seed sinks, and with hand seed is pinched, and pectin is separated with seed, it is removal pulp, miscellaneous without cleaning seed during glutinous sliding sense
Thing and flat seed, load and are squeezed the water out in double gauze bag, are spread out on the overhead canvas in bottom or blotting paper and dry in the shade, and stir several times,
The moisture for making the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%～8%, paper bag packing, room temperature storage is next
The sowing of March in year.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein the determination step of described astaxanthin is to wait to plant
Strain is grown up, and the lower 4-5 floor blades in collection New Tomato Variety ' cloud shrimp No. 3 ' top, freeze-dried acquisition blade dry powder weighs sample
50mg, plus acetone extraction 4 times, until extract is colourless, collects extract and are settled to 5ml, and loading determines pigment after suction filtration, arranges
3 repetitions, with ultra-performance liquid chromatography the content of its astaxanthin is determined.
The breeding method of tomato variety ' cloud shrimp No. 3 ', it comprises the steps：(1) pollen collection：The more maternal early sowing of male parent
20 days, the full-bloom stage morning, collection male parent ' shrimp is red No. 1 ' petal launched 170-190 ° and the partially red flower of pattern after dew is dry, in
Indoor tweezers choose stamen, spread out it is thin and on the blank sheet of paper of flexible, indicate kind name and collection the date, be placed in clean ground
Fang Ziran dries in the shade, and treats Anther dehiscence, when pollen sheds, collects that pollen is standby, and pollen is temporarily stored with 4 DEG C of refrigerator cold-storages, for a long time
Storage silica gel is made drier and is preserved in -80 DEG C of refrigerators；(2) maternal emasculation：Pollination the previous day selects ' cloud is miscellaneous No. 8 ' corolla to stretch
Length exposes, and color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture is bloomed in next day
Bud carries out emasculation, and during emasculation, left hand surely holds bud base portion, and right hand tweezers clamp flower pesticide and all extract its stamen, or even
Together peel off with petal, isolate after emasculation, while rejecting the flower for being not suitable for pollinating and selfing fruit；(3) artificial pollination：It is female daily
Start pollination after Honda dew is dry, carry out in fine day morning 8-9 points, suitable temperature on average of pollinating is 20～25 DEG C, use common
The rubber tip of pencil dips a small amount of pollen, and point is invested on the column cap of maternal emasculation flower or pollen is placed on blank sheet of paper, directly
The column cap of contact emasculation, dips a small amount of pollen, and with 6 × 8cm tea bag bag baggings, paper clip is fixed, and listing mark is indicated on plate
Parent combination number and pollination date, bag taking after pollinating 10 days, the situation while observation is beared fruit, training cleaning in time, removal is not
The bud of pollination and the inflorescence of new life, training is pruned, and extracts unnecessary axillary bud and the branch of excessive growth, carry out liquid manure coordinated management and
The preventing and treating of pest and disease damage；(4) fruit harvesting：50～60 days harvesting ripening fruits, do not harvest unmarked fallen fruits and abnormal after pollination
Shape fruit, only harvests the normal fruit that grows；(5) Seed Treatment：Completely after red ripe fruit harvesting, after-ripening takes again for 1～2 day
Kind, laterally fruit is cut open with knife, seed and glue flesh are clamp-oned in anhydrous nonmetallic vessel and is fermented 1～2 day, until fermentate
Superficial white, is stirred with wooden stick, and seed sinks, and with hand seed is pinched, and pectin is separated with seed, without seed is cleaned during glutinous sliding sense, is used
Clear water is rinsed, and removes pulp, debris and flat seed, is loaded and is squeezed the water out in double gauze bag, is spread out in the overhead canvas in bottom or suction
On water paper, stir, the moisture for making the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%～8%, paper bag packing,
Room temperature storage, the sowing of March in next year；(6) content astaxanthin is determined：New Tomato Variety ' cloud shrimp No. 3 ' after planting, grow up by plant to be planted,
Pluck the lower 4-5 layer blades in top, freeze-dried acquisition blade dry powder weighs sample 50mg, plus acetone extraction 4 times, until extracting
Liquid is colourless, collects extract and is settled to 5ml, and loading determines pigment after suction filtration, 3 repetitions is arranged, using Ultra Performance Liquid Chromatography
UPLC methods determine the content of its astaxanthin.
The breeding method of tomato variety ' cloud shrimp No. 3 ' as mentioned, wherein described with ' 92-23-7A ' as maternal, ' 94-
5-12F ' is female parent for ' cloud is miscellaneous No. 8 ' that paternal hybrid is obtained, and produces astaxanthin engineering tomato ' shrimp is red No. 1 ' and is hybridized for male parent
The biological characteristics of ' the cloud shrimp No. 3 ' that obtain is：' cloud shrimp No. 3 ' is combined with the main merit of Parent, and disease resistance is strong, fruit
Real quality better, high-yield astaxanthin, plant determinate growth, plant height 120-140cm, kermesinus, robust growth, disease resistance is strong；Stem
Yellow green is to light brown, and branch ability level is strong, and leaf yield is high；Common blade profile, petiole yellow green to kermesinus, blade kermesinus,
Its dry astaxanthin average content reaches 2.64mg/g, and calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange
To red, column cap and young fruit brownish red is harbored, fruit large red, fruit shoulder aubergine is circular, fruit shape index 0.8～0.92, fruit face
Smooth, gloss is bright, and pulp is thick, ventricle 5～6, and hardness is big, mean fruit weight 180g, and maximum single fruit weight reaches 400g.
Compared with prior art, excellent benefit of the invention is, the tomato variety cultivated by breeding method of the present invention
' cloud shrimp No. 3 ' with dry matter weight of leaf astaxanthin average content up to 2mg/g as objective trait, take into account Fruit Characteristics, stability and
Whether resistance etc., be kermesinus as the mark property for differentiating Hybrid and ' cloud is miscellaneous No. 8 ' using plant color.See with reference to phenotype
Examine and assessed with the measure of blade content astaxanthin, cloud shrimp No. 3 ' the main merit of Parent is combined with, disease resistance is strong, fruit
Quality better and high-yield astaxanthin.It is mainly characterized by, plant determinate growth, plant height 130cm or so, kermesinus, robust growth,
Disease resistance is strong；Stem yellow green is to light brown, and branch ability is strong, and leaf yield is high；Common blade profile, petiole yellow green to kermesinus, leaf
Piece kermesinus, its dry astaxanthin average content reaches 2.64mg/g.Calyx kermesinus, radial corolla is orange-yellow to Exocarpium Citri Rubrum
Color, flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit large red, fruit shoulder aubergine is circular, fruit shape index 0.8
～0.92, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 5～6, and hardness is big, and mean fruit weight 180g, maximum single fruit weight is reachable
More than 400g.
Specific embodiment：
Further illustrate the essentiality content of the present invention with the concrete instance of the present invention below, but do not limited with this
The present invention.
Embodiment 1：
The hybridizing method of tomato variety ' cloud shrimp No. 3 '：
Composite-crossing method is used, with ' the cloud miscellaneous 8 come as paternal hybrid as maternal, ' 94-5-12F ' by ' 92-23-7A '
Number ' it is female parent, obtained as paternal hybrid with producing astaxanthin engineering tomato ' shrimp is red No. 1 '.
The female parent ' 92-23-7A ' and male parent ' 94-5-12F ' of ' cloud is miscellaneous No. 8 ' is by Yunnan Prov Agriculture University's gardens gardening
The self-mating system of the autonomous seed selection of tomato seminar of institute.Maternal ' 92-23-7A ' fruit quality is excellent, and fruit type is good, individual big.Male parent
' 94-5-12F ' has high disease resistance.Maternal ' cloud is miscellaneous No. 8 ' system is maternal, ' 94-5-12F ' for paternal hybrid by ' 92-23-7A '
, it is Yunnan Province " 15 " Program for Tackling Key Problems《High-quality tomato hybrid new breed seed selection and supporting development technique research》One of achievement,
Support project for Ministry of Science and Technology's agricultural achievements conversion fund simultaneously《High-quality hybrid New Tomato Variety regional testing is demonstrated》Emphasis
New varieties are promoted, seed selection success in 5 years is lasted by tomato project team of gardens College of Horticulture of Yunnan Prov Agriculture University, and passed through Yunnan Province
The authorization of new crop varieties validation board, registration number is：DD001-2003, with independent intellectual property right and adaptation Yunnan
The characteristics of weather conditions.The kind is in multiple spot of the ground through 3 years such as Yunnan Province vegetables main producing region Yuanmou County, Binchuan County and Kunmings
Test, and its disease resistance and interior quality are entered by plant pathology key lab of Yunnan Province and Ministry of Agriculture's detection of agricultural products center
Go and identified and detect, other main breeds have compared with Yunnan Province, showed good.Male parent ' shrimp is red No. 1 ' is to produce astaxanthin work
Journey tomato, cultivated in 2009 and forms, and was that (2013) Huang JC etc. are sieved yellow pretty tide etc. by gene engineering method from green alga
Selecting and be separated to can be catalyzed the assimilation enzyme and '-hydroxylase gene turn of beta carotene and luteole synthesizing astaxanthin in plant cell
In entering wild-type tomatoes plant (cv.UC82B), the transgene tomato for obtaining is (on May 14th, 2014 in China Microbiological bacterium
The common micro-organisms center preservation of preservation administration committee is planted, preserving number is：CGMCC No 9223, Classification And Nomenclature：' shrimp red 1
Number ' transgene tomato).2010 to 2012 respectively in bioscience institute of Hong Kong University and Chinese Academy of Sciences's Kunming plant research
The plantation of 4 generations has been carried out by potted plant mode, proterties is stable.Start sowing at the beginning of 3 months 2013, ' shrimp is red No. 1 ' is than ' cloud is miscellaneous
No. 8 ' early sowing 20 days, May full-bloom stage is pollinated the previous day, and the elongation of ' cloud is miscellaneous No. 8 ' corolla is exposed, and from green to yellow, petal is not for color
Open or crack, flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, the bud emasculation bloomed in next day is clean, notes isolating.The morning
When dew is done, collection male parent ' shrimp is red No. 1 ' petal launches 180 ° or so and the partially red flower of pattern, and in indoor tweezers hero is chosen
Stamen, spread out dry in the air it is thin and on the blank sheet of paper of flexible, indicate the collection date, be placed in it is clean where dry in the shade naturally.Treat Anther dehiscence,
When pollen sheds, pollen is collected standby.Second day, after maternal field dew is dry, fine day was pollinated in morning 8-9 points.Specifically,
A small amount of pollen point being dipped with the rubber tip of common pencil and investing be placed on blank sheet of paper on the column cap of maternal emasculation flower or by pollen,
The column cap of contact emasculation, directly dips a small amount of pollen.With 6 × 8cm tea bag bag baggings, paper clip is fixed, listing mark, on plate
Indicate Parent combination number and pollination date.Bag taking after pollinating 10 days, the situation while observation is beared fruit, in time training cleaning, goes
Except the inflorescence of the bud and new life do not pollinated, training is pruned, and extracts unnecessary axillary bud and the branch of excessive growth, and period notices that liquid manure is cooperateed with
Management and the preventing and treating of pest and disease damage.Ripening fruits can be harvested within about 50～60 days after pollination.Do not harvest unmarked fallen fruits and
Malformed fruit, only harvests the normal fruit that grows.Completely after red ripe fruit harvesting, can after-ripening take kind again within 1～2 day.Take kind
When, laterally fruit is cut open with knife, then seed and glue flesh are clamp-oned in anhydrous nonmetallic vessel and is fermented, ferment 1～2 day
After clean seed, be placed in divulging information, be dried where be filtered dry moisture and dry immediately, until seed moisture content is down to 7%～8%
When preserve, March in next year sowing.Treat that tomato variety ' cloud shrimp No. 3 ' plant after planting grows up, the lower 4-5 layer leaves in its top of timely collecting
Piece, freeze-drying obtains dry powder.Accurately weigh sample 50mg, plus acetone extraction 4 times, until extract is colourless, collect extract
5ml is settled to, loading determines pigment after suction filtration, 3 repetitions are set, its shrimp is determined using Ultra Performance Liquid Chromatography (UPLC) method blue or green
The content of element.4 times through 2014 and 2015 are planted, and the measurement result of Phenotypic Observation and blade content astaxanthin shows,
' cloud shrimp No. 3 ' proterties is excellent neat, and fruit quality is good, and blade content astaxanthin is high, and disease resistance is strong.
The proterties of the hybrid strain that hybridizing method of the present invention is related to is as follows：
' cloud is miscellaneous No. 8 '：Determinate growth, 130～150cm of plant height, growth is strong, and disease resistance is strong, belongs to Early-medium maturing variety；Really
Real large red, uniform coloring, high circular, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 4～5, and hardness is big, and storage tolerance is good,
Shelf life is long, average fruit weight 210g, maximum fruit mass up to more than 500g, in Kunming Areas spring greenhouse cultivation, from
Harvesting is seeded into for 180 days or so；Yuanmou area July is seeded into harvesting needs 130 days or so.Outdoor cropping is per 667m2Produce
, up to more than 6000kg, Protectorate cultivation is per 667m for amount2Yield is up to more than 7000kg.Food flavor is good, and sandy taste is sweet, is dish use kind
Eggplant improved seeds, while as processing, filling cultivation.
Produce astaxanthin engineering tomato ' shrimp is red No. 1 '：Its plant, flower, leaf, fruit morphologically with Lycopersicon esculentum indifference, it is main
If the difference in color.Specifically, plant one dimensional infinite wall, plant height 110cm or so.Stem be half erect type, thick 2.6～
4.7cm, 4～6 branches, yellow green is to light brown.Common blade profile, petiole green is to kermesinus, blade kermesinus.Hermaphrodite flower,
Calyx kermesinus, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit
It is subsphaeroidal, Chinese red after maturation, mean fruit weight 100g or so, the mean value of its fruit content astaxanthin is 1.63mg/g.
New varieties ' cloud shrimp No. 3 '：The new varieties ' cloud shrimp No. 3 ' that Jing composite-crossings are cultivated are combined with the master of 3 parents
Merit is wanted, disease resistance is strong, fruit quality is good and high-yield astaxanthin.It is mainly characterized by, plant determinate growth, plant height
130cm or so, kermesinus, robust growth, disease resistance is strong；Stem yellow green is to light brown, and branch is very competent, and leaf yield is high；It is general
Logical blade profile, petiole yellow green to kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.64mg/g.Calyx is dark
Redness, radial corolla is orange-yellow to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red.Fruit large red,
Fruit shoulder aubergine, circular, fruit shape index 0.8～0.92, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 5～6, and hardness is big, puts down
Single fruit weight 180g, maximum single fruit weight is up to more than 400g.
Claims (10)
1. the breeding method of tomato variety ' cloud shrimp No. 3 ', including pollen collection, maternal emasculation, artificial pollination, fruit harvesting, kind
Subprocessing, content astaxanthin determination step, the method is obtained for paternal hybrid with ' 92-23-7A ' as maternal, ' 94-5-12F '
' cloud is miscellaneous No. 8 ' for female parent, produce astaxanthin engineering tomato ' shrimp is red No. 1 ' carry out hybridization cultivation ' cloud shrimp No. 3 ' for male parent, use acetone
Blade astaxanthin is extracted, its content is detected using UPLC methods.
2. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 1, it is characterised in that：Described pollen collection
Step is that than maternal early sowing 20 days, the full-bloom stage morning, collection male parent ' shrimp is red No. 1 ' petal launched 170- to male parent after dew is dry
190 ° and the partially red flower of pattern, choose stamen, spread out on blank sheet of paper, indicate kind name and collection date, dry in the shade naturally, treat flower pesticide
Split, when pollen sheds, collect pollen standby；Described maternal emasculation selects the elongation of ' cloud is miscellaneous No. 8 ' corolla for pollination the previous day
Expose, color from green to yellow, petal is not opened or crack, flower pesticide sheds pollen, the flower bloomed in next day in yellow green but do not ftracture
Flower bud carries out emasculation；Described artificial pollination is after maternal field dew is dry, to award in fine day morning 8-9 points, 20～25 DEG C of temperature
Powder；Described fruit harvesting is 50～60 days harvesting ripening fruits after pollination；Described Seed Treatment is complete red ripe fruit
After real harvesting, after-ripening takes again kind in 1～2 day；It is with acetone extraction blade astaxanthin, the inspection of UPLC methods that described content astaxanthin is determined
Survey its content.
3. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described pollen collection
The pollen collected in step is temporarily stored with 4 DEG C of refrigerator cold-storages, and long-term storage silica gel makees -80 DEG C of Refrigerator stores of drier.
4. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described maternal emasculation
Step is surely to hold bud base portion with left hand, and right hand tweezers clamp flower pesticide and all extract its stamen, or together shell together with petal
Fall, emasculation is clean, isolate after emasculation, while rejecting the flower for being not suitable for pollinating and selfing fruit.
5. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described artificial pollination
Step temperature is 20～25 DEG C, dips a small amount of pollen point with the rubber tip of common pencil and invests on the column cap of maternal emasculation flower,
Or directly pollen is placed on paper, the column cap of directly contact emasculation dips a small amount of pollen.It is bent with 6 × 8cm tea bag bag baggings
Safety pin is fixed, listing mark, and Parent combination number and pollination date are indicated on plate, bag taking after pollinating 10 days, while observation
Bear fruit situation, in time training cleaning, remove the inflorescence of the bud and new life not pollinated, training is pruned, and extracts unnecessary axillary bud
And the branch of excessive growth, carry out liquid manure coordinated management and the prevention and control of plant diseases, pest control.
6. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described fruit harvesting
When only harvest the normal fruit that grows, do not harvest unmarked fallen fruits and malformed fruit.
7. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described Seed Treatment
Step is laterally open to be cut fruit with knife, seed and glue flesh is clamp-oned in anhydrous nonmetallic vessel and is fermented 1～2 day, to fermentation
Thing surface is white, is stirred with wooden stick, and seed sinks, and with hand seed is pinched, and pectin is separated with seed, without cleaning kind during glutinous sliding sense
Son, removes pulp, debris and flat seed, loads and is squeezed the water out in double gauze bag, is spread out on the overhead canvas in bottom or blotting paper
Dry in the shade, stir several times, the moisture for making the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%～8%, paper bag bag
Dress, room temperature storage, the sowing of March in next year.
8. the breeding method of tomato variety ' cloud shrimp No. 3 ' as claimed in claim 2, it is characterised in that：Described astaxanthin
Determination step is that plant to be planted is grown up, the lower 4-5 floor blades in collection New Tomato Variety ' cloud shrimp No. 3 ' top, freeze-dried acquisition blade
Dry powder, weighs sample 50mg, plus acetone extraction 4 times, until extract is colourless, collects extract and is settled to 5ml, loading after suction filtration
Pigment is determined, 3 repetitions are set, with ultra-performance liquid chromatography the content of its astaxanthin is determined.
9. the breeding method of tomato variety ' cloud shrimp No. 3 ', it is characterised in that it comprises the steps：(1) pollen collection：Male parent compared with
Maternal early sowing 20 days, the full-bloom stage morning, collection male parent ' shrimp is red No. 1 ' petal launches 170-190 ° and pattern is partially red after dew is dry
Flower, choose stamen in indoor tweezers, spread out it is thin and on the blank sheet of paper of flexible, indicate kind name and collection the date, be placed in
Dry in the shade naturally where clean, treat Anther dehiscence, when pollen sheds, collection pollen is standby, and pollen is temporarily stored cold with 4 DEG C of refrigerators
Hide, long-term storage silica gel is made drier and preserved in -80 DEG C of refrigerators；(2) maternal emasculation：Pollination the previous day selects ' cloud miscellaneous 8
Number ' corolla elongation exposes, color from green to yellow, do not open or crack by petal, and flower pesticide is in yellow green, but the pollen that sheds that do not ftracture, in
The bud that next day blooms carries out emasculation, and during emasculation, left hand surely holds bud base portion, and right hand tweezers clamp flower pesticide by its stamen whole
Extract, or together peel off together with petal, isolate after emasculation, while rejecting the flower for being not suitable for pollinating and selfing fruit；(3) manually award
Powder：Start pollination after maternal field dew is dry, carry out in fine day morning 8-9 points, suitable temperature on average of pollinating is 20～25 DEG C,
A small amount of pollen is dipped with the rubber tip of common pencil, point is invested on the column cap of maternal emasculation flower, or pollen is placed in into thin letter
On writing paper paper, the column cap of directly contact emasculation dips a small amount of pollen, and with 6 × 8cm tea bag bag baggings, paper clip is fixed, and be listed mark
Note, indicates Parent combination number and pollination date on plate, bag taking after pollinating 10 days, the situation while observation is beared fruit, whole in time
Branch cleaning, removes the inflorescence of the bud and new life not pollinated, and training is pruned, and unnecessary axillary bud and the branch of excessive growth is extractd, while entering
Water-filling fertilizer coordinated management and the prevention and control of plant diseases, pest control；(4) fruit harvesting：50～60 days harvesting ripening fruits, do not harvest nothing after pollination
The fallen fruits and malformed fruit of mark, only harvest the normal fruit that grows；(5) Seed Treatment：Complete red ripe fruit harvesting
Afterwards, after-ripening takes again kind in 1～2 day, and with knife fruit is laterally cut open, seed and glue flesh is clamp-oned and ferments 1 in anhydrous nonmetallic vessel
～2 days, until fermentate superficial white, is stirred with wooden stick, seed sank, and with hand seed is pinched, and pectin is separated with seed, without glutinous cunning
Seed is cleaned during sense, pulp, debris and flat seed is removed, is loaded and is squeezed the water out in double gauze bag, be spread out in the overhead canvas in bottom
Or on blotting paper, stir, the moisture for making the surface of the seed is evaporated rapidly, until when seed moisture content is down to 7%～8%, paper bag
Packaging, room temperature storage, the sowing of March in next year；(6) content astaxanthin is determined：After planting, plant to be planted is long for tomato variety ' cloud shrimp No. 3 '
Greatly, the lower 4-5 layer blades in top are plucked, freeze-dried acquisition blade dry powder weighs sample 50mg, plus acetone extraction 4 times, until taking out
Extract is colourless, collects extract and is settled to 5ml, and loading determines pigment after suction filtration, 3 repetitions is arranged, using ultra high efficiency liquid phase color
Spectrum UPLC methods determine the content of its astaxanthin.
10. the breeding method of tomato variety ' cloud shrimp No. 3 ' as described in claim 1 or 9, it is characterised in that it is described with
' 92-23-7A ' is female parent for ' cloud is miscellaneous No. 8 ' that paternal hybrid is obtained for maternal, ' 94-5-12F ', produces astaxanthin engineering tomato
' shrimp is red No. 1 ' is for the biological characteristics that male parent carries out hybridizing ' the cloud shrimp No. 3 ' that obtain：' cloud shrimp No. 3 ' is combined with Parent
Main merit, disease resistance is strong, fruit quality is good, and high-yield astaxanthin, plant determinate growth, plant height 120-140cm is dark red
Color, robust growth, disease resistance is strong；Stem yellow green is to light brown, and branch ability is strong, and leaf yield is high；Common blade profile, petiole yellow green
To kermesinus, blade kermesinus, its dry astaxanthin average content reaches 2.64mg/g, calyx kermesinus, radial corolla orange
Yellow is to Chinese red, and flower pesticide is orange to red, column cap and harbors young fruit brownish red, and fruit large red, fruit shoulder aubergine is circular,
Fruit shape index 0.8～0.92, fruit surface smooth, gloss is bright, and pulp is thick, ventricle 5～6, and hardness is big, mean fruit weight 180g, most
Big single fruit weight reaches 400g.
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| CN112544439A (en) * | 2020-12-28 | 2021-03-26 | 江苏省农业科学院 | Exquisite tomato cross pollination method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030126649A1 (en) * | 2002-01-03 | 2003-07-03 | Kuehn Michael Edwin | Inbred tomato line 294 |
| CN104145678A (en) * | 2014-08-15 | 2014-11-19 | 中国科学院昆明植物研究所 | Cultivation method for promoting growth of transgenic tomatoes and accumulation of astaxanthin |
| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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| US20030126649A1 (en) * | 2002-01-03 | 2003-07-03 | Kuehn Michael Edwin | Inbred tomato line 294 |
| CN104145678A (en) * | 2014-08-15 | 2014-11-19 | 中国科学院昆明植物研究所 | Cultivation method for promoting growth of transgenic tomatoes and accumulation of astaxanthin |
| CN104206139A (en) * | 2014-08-15 | 2014-12-17 | 中国科学院昆明植物研究所 | Method for improving yield of transgenic tomato containing astaxanthin and content of astaxanthin in transgenic tomato |
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| 朱海山等: "云杂8号番茄杂交新品种选育及栽培技术", 《中国种业》 * |
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| CN112544439A (en) * | 2020-12-28 | 2021-03-26 | 江苏省农业科学院 | Exquisite tomato cross pollination method |
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