CN104351170A - Shrub willow pollen preservation method - Google Patents
Shrub willow pollen preservation method Download PDFInfo
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- CN104351170A CN104351170A CN201410491983.3A CN201410491983A CN104351170A CN 104351170 A CN104351170 A CN 104351170A CN 201410491983 A CN201410491983 A CN 201410491983A CN 104351170 A CN104351170 A CN 104351170A
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Abstract
The invention discloses conditions for preservation of shrub willow pollen, comprising the following steps: (1) drying; and (2) low-temperature preservation. By the shrub willow pollen preservation method, aging of pollen can effectively be prevented, respiration intensity and enzymatic activity of pollen can be reduced, vitality of pollen can be guaranteed; hybridization difficulty of the pollen due to dioecism and flowering asynchronism of shrub willow pollen can be overcome; and vitality still can reach up to 49% when shrub willow pollen is preserved to 180 d at low temperature. The shrub willow pollen preservation method provided by the invention is simple and easy to operate and is low-cost. By the preservation method, hybridization vitality of shrub willow pollen can effectively be guaranteed. The preservation method is of great practical and promotional significance in propagation and production of shrub willow.
Description
Technical field
The present invention relates to a kind of plant pollen store method, specifically relate to a kind of method of shrub pollen for low temperature storage.
Background technology
Salix (Salix L.) is the maximum genus of Salicaceae, and about 550 kinds, China sallow 255 kinds, accounts for 46% of whole world sum, be whole world sallow at most, the abundantest area, extensively distribute in national each province, district.And the Salix overwhelming majority is shrub willow, numerous species is sad relevant to people's productive life.Along with the development of global economy, people, to the progressively understanding of shrub willow effect, particularly after Doha meeting in 2008, have deep love for nature, advocate environmental protection, advocate the common recognition that low-carbon economy has become whole world national governments and the people, particularly urgent and important to the exploitation of shrub willow.
Pollen is as one of the form of shrub willow kind matter, and containing all gene types of willow, have abundant genetic diversity, is the important materials of willow preserving seed and crossbreeding.But willow dioecism, flowering asynchronism, and the willow pollen natural conditions lower life-span is short, this brings great inconvenience to the matter research of willow kind and crossbreeding work thereof, is also unfavorable for the genetic improvement research of willow simultaneously.Therefore, be necessary to preserve willow pollen to carry out correlative study.Although willow pollen collection having application with being housed in the breeding of European willow, it being extracted and preserves method does not specifically describe.The object that research preserved by pollen is to keep pollen viability under the prerequisite not destroying germ plasm resource, extend its fertility, and the restriction of the Time and place in crossbreeding is overcome with this, improve breeding efficiency, for interzone and even international kind of mass transter are provided convenience; The propagation of damage by disease and insect can be avoided simultaneously.Such as willow dioecism, and male and female flowering time is not identical, natural breeding causes flowering asynchronism, and in order to overcome this difficult problem, the preservation of its pollen just has the necessity of reality.
Summary of the invention
The object of the invention is to determine shrub willow pollen optimum drying method, drying time and Cord blood temperature.
The present invention is achieved through the following technical solutions:
Step (1) is by any one in shrub willow pollen employing natural air drying, light drying, desiccant dryness three kinds of modes or any two or three mode drying.
Step (2) shrub willow pollen, after step (1) process, is placed in-70 ~ 4 degrees Celsius of preservations.
Further, step (1) collects shrub willow fresh pollen, natural air drying is that Indoor Natural dries, the dry concrete operations of light are that under pollen being placed in 30W incandescent lamp, 5 ~ 8cm place is irradiated, desiccant dryness is put in drier by 500g discolour silica gel, put a dividing plate from discolour silica gel 2cm place, then pollen is placed on dividing plate.
The described natural air drying 2 ~ 24h of step (1), light drying 0.5 ~ 6h, desiccant dryness 1 ~ 16h;
In step (2), described the shrub willow pollen of drying process to be mixed, be evenly divided in 5mL centrifuge tube, after 4 degrees Celsius of refrigeration 1h, then after proceeding to-25 degrees Celsius of freezing 2h, be placed in-70 degrees Celsius of preservations.
The invention has the beneficial effects as follows:
1, shrub willow pollen store method of the present invention is adopted can effectively to stop pollen old and feeble, reduce pollen respiratory intensity and enzymic activity, ensure pollen viability, overcome the hybridization difficulty that shrub willow pollen causes because of dioecism flowering asynchronism, when shrub willow pollen is saved to 180d in low temperature, vitality still can up to 49%.
2, adopt shrub willow pollen store method of the present invention compared with Excised Embryos, simple to operate, cost is low, and Cord blood also can reduce the genetic variation that ultralow temperature produces.
3, adopt shrub willow pollen store method of the present invention, drying means is rapid dry process, and drying time is short, also ensure that pollen is unlikely to complete deactivation, is conducive to preserving.And dry input cost is also lower.In sum: shrub willow pollen store method of the present invention is simple to operation, and cost is low, effectively can ensure that shrub willow pollen carries out the vitality of hybridizing, shrub willow breeding production have important practice and dissemination.
Accompanying drawing explanation
Fig. 1 is storage temperature and shrub willow pollen viability;
Fig. 2 is the impact that pre-freeze process is preserved shrub willow pollen.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment, the present invention is described in further detail.
Embodiment: shrub willow pollen for low temperature storage method, comprises the steps:
(1) select drying mode and drying time: collect fresh shrub willow pollen, mixing, is divided into some parts, uses three kinds of drying mode dryings respectively.
The drying means of table 1 willow pollen and drying time
In upper table, P63 represents Salix suchowensis; P102 represents silver-colored willow; 2333 represent wormwood artemisia willow X hair branch willow; 2383 represent Salix suchowensis X wormwood artemisia willow;
The selection result is as follows:
Silver willow pollen the best is desiccant dryness 4h;
Salix suchowensis pollen the best is desiccant dryness 4h;
Salix suchowensis × wormwood artemisia willow pollen the best is natural air drying 2h;
Mao Zhiliu × wormwood artemisia willow pollen the best is desiccant dryness 2h.
(2) by dried silver-colored willow pollen, Salix suchowensis pollen, Salix suchowensis × wormwood artemisia willow pollen, Mao Zhiliu × wormwood artemisia willow pollen is put into room temperature respectively, spends and preserve in-70 degree for 4 degree ,-25.
The selection result drawn from Fig. 1 is as follows:
The best storage temperature of silver willow pollen is-70 degree.
The best storage temperature of Salix suchowensis pollen is-70 degree.
The best storage temperature of Salix suchowensis × wormwood artemisia willow pollen is-70 degree.
The best storage temperature of Mao Zhiliu × wormwood artemisia willow pollen is-70 degree.
(3) pre-freeze process is selected to preserve: by silver-colored willow pollen, Salix suchowensis pollen, Salix suchowensis × wormwood artemisia willow pollen, Mao Zhiliu × wormwood artemisia willow pollen carries out following operation respectively: after pollen drying, be divided in 5mL centrifuge tube, after 4 degree of refrigeration 1h, then after proceeding to the freezing 2h of-25 degree, be placed in-70 degree and preserve.
After said method is preserved, as can be seen from Figure 2:
The germination rate that silver willow pollen is saved to 180 days is 39.1%.
The germination rate that Salix suchowensis pollen is saved to 180 days is 49.2%.
The germination rate that Salix suchowensis × wormwood artemisia willow pollen is saved to 180 days is 40.9%.
The germination rate that Mao Zhiliu × wormwood artemisia willow pollen is saved to 180 days is 53.6%.
The above discloses the present invention with preferred embodiment.So it is not intended to limiting the invention, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (8)
1. a shrub willow pollen for low temperature storage method, is characterized in that, comprise the steps:
(1) shrub willow pollen is adopted respectively the drying of natural air drying, light, desiccant dryness three kinds of mode dryings;
(2) shrub willow pollen is after step (1) process, is placed in-70 ~ 4 degrees Celsius of preservations.
2. shrub willow pollen for low temperature storage method according to claim 1, is characterized in that: described step (1) filters out optimum drying mode.
3. shrub willow pollen for low temperature storage method according to claim 1 or 2, is characterized in that: the drying of described light adopts the incandescent lamp of 30w, and pollen is placed in 5 ~ 8cm place under lamp.
4. shrub willow pollen for low temperature storage method according to claim 3, is characterized in that: the time of described light drying is 0.5 ~ 6h.
5. shrub willow pollen for low temperature storage method according to claim 1, is characterized in that: the method for described desiccant dryness is: use discolour silica gel as desiccant, pollen is placed in drier dry.
6. shrub willow pollen for low temperature storage method according to claim 5, is characterized in that: the time of described desiccant dryness is 1 ~ 16h.
7. shrub willow pollen for low temperature storage method according to claim 1, is characterized in that: the natural air drying drying time described in step (1) is 2 ~ 24h.
8. shrub willow pollen for low temperature storage method according to claim 1, it is characterized in that: in step (2), first through the shrub willow pollen of step (1) drying process prior to after 4 degrees Celsius of refrigeration 1h, then after proceeding to-25 degrees Celsius of freezing 2h ,-70 degrees Celsius of preservations will be placed in.
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| CN201410491983.3A CN104351170B (en) | 2014-09-23 | 2014-09-23 | A kind of shrub willow pollen store method |
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| CN201410491983.3A CN104351170B (en) | 2014-09-23 | 2014-09-23 | A kind of shrub willow pollen store method |
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| CN104351170B CN104351170B (en) | 2017-08-08 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105961377A (en) * | 2016-06-06 | 2016-09-28 | 广东省林业科学研究院 | Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability |
| CN106036361A (en) * | 2016-08-22 | 2016-10-26 | 文山苗乡三七科技有限公司 | Panax notoginseng pollen storage method |
| CN106576965A (en) * | 2016-12-06 | 2017-04-26 | 江苏沿江地区农业科学研究所 | Cryopreservation and resuscitation method of willow breeding material |
| CN107484755A (en) * | 2017-08-09 | 2017-12-19 | 合肥沁牧生态农业有限公司 | A kind of walnut male flower pollen store method |
| CN113465298A (en) * | 2021-07-16 | 2021-10-01 | 四川农业大学 | South Asia loquat pollen drying and storing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961377A (en) * | 2016-06-06 | 2016-09-28 | 广东省林业科学研究院 | Castanopsis hystrix pollen collecting and storing method for increasing germination rate and improving pollen viability |
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| CN106036361A (en) * | 2016-08-22 | 2016-10-26 | 文山苗乡三七科技有限公司 | Panax notoginseng pollen storage method |
| CN106576965A (en) * | 2016-12-06 | 2017-04-26 | 江苏沿江地区农业科学研究所 | Cryopreservation and resuscitation method of willow breeding material |
| CN107484755A (en) * | 2017-08-09 | 2017-12-19 | 合肥沁牧生态农业有限公司 | A kind of walnut male flower pollen store method |
| CN113465298A (en) * | 2021-07-16 | 2021-10-01 | 四川农业大学 | South Asia loquat pollen drying and storing method |
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| CN104351170B (en) | 2017-08-08 |
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Address after: Jiangsu forestry building, 22 Dinghuaimen street, Gulou District, Nanjing, Jiangsu 210036 Patentee after: JIANGSU ACADEMY OF FORESTRY Address before: 211153 Jiangsu Nanjing Jiangning District East good bridge Patentee before: JIANGSU ACADEMY OF FORESTRY |
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