CN102640631B - Cuttage seedling culture method for honeysuckle - Google Patents

Cuttage seedling culture method for honeysuckle Download PDF

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CN102640631B
CN102640631B CN201110042193.3A CN201110042193A CN102640631B CN 102640631 B CN102640631 B CN 102640631B CN 201110042193 A CN201110042193 A CN 201110042193A CN 102640631 B CN102640631 B CN 102640631B
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cuttage
cuttings
honeysuckle
seconds
growth regulator
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CN201110042193.3A
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CN102640631A (en
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张燕
兰金宝
翁炜
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北京灏銮科技发展有限公司
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Abstract

The invention discloses a cuttage seedling culture method for honeysuckle. During the cuttage in the open air, the cuttage season is selected to be August and September; under the small shed building moisture retaining condition, the cuttage is carried out in October and November; cutting scions need to be lignified and are not infected with diseases or pests, two to six sections are remained, and all leaves are removed; the cutting scion diameter is greater than or equal to 0.5cm, is preferably greater than or equal to 0.6cm and is more preferably 0.7cm; and before the cuttage, the cutting scions are dipped with 30-200mg/L growth regulators for 5 seconds to 2 minutes. After the method provided by the invention is adopted, the cuttage survival rate of honeysuckle seedlings is effectively improved.

Description

The cuttage and seedling culture method of a kind of honeysuckle
Technical field
The present invention relates to agricultural herding, cultivation field, relates in particular to the cuttage and seedling culture method of a kind of honeysuckle.
Background technology
Honeysuckle is the caprifoliaceae plant dry flower of (Lonicera japonica Thunb.) honeysuckle, is clearing heat and detoxicating conventional Chinese medicine, cures mainly anemopyretic cold, abscess of throat.Main product in Shandong, the province such as Henan, Hebei, with Hebei output maximum.At present, honeysuckle is in disease prevention and cure, and the market demand capacity of the aspects such as health products, tea-drinking is large, has good economic benefit.In addition, checking winds and fixing drifting sand, solving work problem and the aspect such as increase farmers' income of older rural labour and housewife and there is good ecological environment benefit and social benefit.The cultivated area of honeysuckle also increases year by year, and supply falls short of demand for honeysuckle nursery stock.
The cottage propagation of honeysuckle has easily and survives, and speed is fast, and the feature such as simultaneously use manpower and material resources sparingly, therefore produces the upper cottage propagations that adopt more.But in traditional cottage propagation process, have following defect: 1, cuttage Season select is bad, and cuttage envirment factor is grasped bad; 2, cuttings selection and classification are not strict; 3, cuttings processing mode is improper.Thereby cause the low even difficulty of cuttage seeding survival rate to survive.Therefore the honeysuckle cuttage and seedling culture method that urgently provides a kind of cuttage seeding survival rate to be improved and to ensure.
Summary of the invention
For the problems referred to above that exist in current honeysuckle propagation technique, the inventor is through studying with keen determination discovery, and the selection in cuttage season is to improve one of key factor of honeysuckle cuttage and seedling culture; In cuttage environmental factor, temperature, humidity especially will be controlled well.Cuttings need to, by stepped diameter, can not select not meet the cuttings of diameter requirement, and will control length.The root-inducing powder concentration for the treatment of of cuttings and time are the key factors of cuttage rooting speed and quantity.
Therefore, the object of this invention is to provide the cuttage and seedling culture method of a kind of honeysuckle.Apply method of the present invention and not only can improve gold and silver flower seedling cuttage survival rate, and growth rate is accelerated.
The cuttage and seedling culture method of honeysuckle of the present invention comprises following characteristics:
A, cuttage Season select: in the situation of cuttage in the open, cuttage is selected in eight season, the September; In the situation that taking hut, ten, the November carries out cuttage;
B, cuttings are selected: the necessary lignification of cuttings, and do not infect damage by disease and insect, stay 2 to 6 to save and remove all blades; Diameter >=0.5 centimetre of cuttings, preferably >=0.6 centimetre;
C, cuttings treatment conditions select: before cuttage, cuttings is dipped in the growth regulator 5 seconds to 2 minutes of 30 to 200 mg/litre.
Embodiment
In the cuttage process of honeysuckle, surviving and root of hair of cuttings, is subject to the impact of temperature and humidity very large.In production, do not control well due to cutting time and temperature and humidity, cause situation about not surviving on a large scale a lot.The present invention, by carrying out cuttage test in different time, Various Seasonal such as March, June, July, August, September, October, November, finds best cutting time and temperature and humidity condition.
Experiment finds, for the situation of cuttage in the open, best cuttage season is in August, September, and this, weather temperature was high in season, and cuttings is in large supply, and survival rate is high, and cost is little.In addition, although October and November temperature low, humidity is little, if take little shed, can play insulation, propose wet effect, can reach equally more than 75% survival rate, but scaffolding cost can correspondingly uprise.March temperature low, humidity is little, is unsuitable for cuttage.Although June and July temperature height, cuttings is in vegetative period, degree of lignification is not high, be unfavorable for surviving, this season is also unsuitable for cuttage.
It is that cuttage seeding survives fast that cuttings is selected, and can obtain the precondition of healthy and strong new plant.In cuttings collection process, often find the cuttings that some do not have lignification or infected damage by disease and insect, surviving of they is low, or plant is small and weak, unhealthy and strong.
For this reason, the present invention carries out lot of experiments to different size cuttings, checks, screens cuttings condition with the survival rate of cuttage seeding:
First, cuttings must lignification, stays 2 to 6 joints, preferably stays 3 to 5 joints, optimum 4 joints of selecting and remain.Because cuttings is beneficial to and sprouts without leaf, therefore remove all blades.
It is wooden that " lignification " described herein meaning refers to that the cuttings of honeysuckle has become, and this can be shown by the diameter >=0.25cm of honeysuckle cuttage fringe.
Secondly, cuttings is more slightly more beneficial to and sprouts, therefore the diameter of cuttings preferably >=0.5 centimetre, more preferably >=0.6 centimetre, most preferably >=0.7 centimetre.Found through experiments, carry out classification for cuttings and be necessary.Generally carry out classification by diameter, diameter >=0.7 centimetre is one-level, and 0.5 centimetre≤diameter≤0.7 centimetre is secondary, and 0.5 centimetre of diameter < is three grades.
In addition, emphasize to quarantine in cuttings collection process, select branch that robust growth do not have damage by disease and insect as cuttings.
According to test, in conjunction with practice experience, summary to the requirement of cuttings is: the necessary lignification of cuttings, and do not infect damage by disease and insect, stay 4 joints best.Diameter >=0.7cm is one-level, and 0.5cm≤diameter < 0.7cm is secondary, and diameter < 0.5cm is three grades.
Select after the cuttings that meets above requirement, directly do not carry out cuttage, generally also need to process.In production practices, if do not carry out respective handling, can cause that cuttage seeding survival rate is low, poor growth, thereby miss, the best survives and the season of growth.Therefore, the processing of cuttings is to be related to that cuttage seeding survives the committed step of speed and root of hair amount.
According to the present invention, the processing of cuttings is comprised to the measures such as sterilization, hestening rooting.
There is no special requirement for sterilization, can use the conventional mode in this area to carry out disinfection.
The present invention, by adopting Orthogonal Experiment and Design, investigates different types of growth regulator, variable concentrations and the impact of different disposal time on cuttage seedling, finds best cuttings processing method.
Particularly, cuttings is processed with growth regulator, thereby promoted sprouting and radical after cuttings cuttage.Optional growth regulator is indolebutyric acid (IBA), root-inducing powder (ABT), heteroauxin (IAA), gibberellin (GA3), methyl α-naphthyl acetate (NAA) or 6-benzyl aminopurine (6-BA), preferably adopt indolebutyric acid (IBA) or root-inducing powder (ABT), and contrast with clear water CK disposition.Experiment is found, is dipped in root-inducing powder ABT and survival rate can be brought up to 78% by 67%; Dip in methyl α-naphthyl acetate NAA and root-inducing powder ABT and cuttage seeding radical can be brought up to respectively to 8.6 and 7.7 by 4.7.
The concentration of growth regulator is generally 30 to 200 mg/litre, preferably 50 to 150 mg/litre, most preferably 80 to 120 mg/litre, for example 100 mg/litre.
The time that cuttings is dipped in growth regulator is generally 5 seconds to 2 minutes, and preferably 10 seconds to 1 minute, most preferably 15 seconds to 30 seconds, for example 20 seconds.
The honeysuckle cuttage seeding rooting rate obtaining by the inventive method is high, and seedling strain is sturdy.From nursery goes out garden, transplant behind land for growing field crops, substantially can both survive, and growth situation is better than the honeysuckle cuttage seeding of cultivating by conventional method.
Preferably, thus for preventing that root system dehydration from affect cuttage seeding and surviving, cuttage seeding from nursery goes out garden to transplanting into taking following measures in the process of land for growing field crops:
For in-plant transplanting situation: honeysuckle nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time, in 48 hours, is dipped in thin mud by seedling root in transportation, so just can protect root system not dewater.
For remote transplanting situation: honeysuckle nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time exceedes 48 hours, or to store temporarily, seedling root is dipped to the moisturizing mud that clay, water-loss reducer and water mix.Why selecting water-loss reducer, is because the water content of water-loss reducer is larger, even and use powdery water-loss reducer, after water suction, still keep intrinsic many diamond shaped, in moisturizing, can also allow root system carry out eupnea.
Water-loss reducer claims again SAP, the reagent often adopting for water conservation in plant cultivation and horticultural management, main component is generally the EVA of polyacrylate (sylvite or sodium) or itself and polyacrylamide, there is water sorption, water suction multiple is 150~400 times, can give plant water keeping, fertilizer conservation and insulation.The present invention can use conventional water-loss reducer, for example commercially available various water-loss reducers of buying, and as the wing centre section board water-loss reducer of Yongkang City, Zhejiang, peace letter water-loss reducer of Dongguan City etc.
The configuration proportion of moisturizing mud is generally: particulate water-loss reducer: clay: water=1: 50-200: 100-300, preferably this ratio is: particulate water-loss reducer: clay: water=1: 100-180: 150-250, most preferably this ratio is: particulate water-loss reducer: clay: water=1: 120-160: 180-220, aforementioned proportion is volume ratio.
The present invention is described in more detail by the following examples.
Embodiment 1:
Various Seasonal is mainly the impact of temperature and humidity on cuttage test survival rate
Obtain lignification cuttings in Various Seasonal, carry out cuttage test: cuttings does not infect damage by disease and insect, stay 4 to save and remove all blades, the diameter of cuttings is 0.7 centimetre, and cuttings is dipped in the growth regulator root-inducing powder 30 seconds of 100 mg/litre, and then cuttage.Then observe its survival rate, result is as shown in table 1:
Table 1 Various Seasonal cuttage test survival rate application form
Can find out according to the data in table 1, under the condition of cuttage in the open, best cuttage season is in August, September, and this, weather temperature was high in season, and cuttings is in large supply, and survival rate is high, and cost is little.In addition, although October and November temperature low, humidity is little, test find, by taking little shed, can play insulation, propose wet effect, can reach equally more than 75% survival rate.
March temperature low, humidity is little, is unsuitable for cuttage.Although June and July temperature height, cuttings is in vegetative period, degree of lignification is not high, be unfavorable for surviving, this season is also unsuitable for cuttage.
Embodiment 2:
The impact of cuttings condition on cuttage seeding survival rate
Between the August to September, obtain the cuttings of different size, then adopt identical operation by its cuttage in nursery, survey respectively its cuttage seeding survival rate, find the grade scale of different size cuttings.
This test adopts three factor randomised block design, investigates the impact of cuttings quality on cuttage seedling, and processing and level are as follows:
A-degree of lignification (diameter), a1:0.3cm, a2:0.5cm, a3:0.7cm;
B-branch joint number, b1:3 joint, b2:4 joint, b3:5 joint;
Whether C-has leaf, c1: have leaf, c2: without leaf.
Table 2 cuttings proterties level design table
Because each processing has 30 repetitions, so data volume is huge, draw the lower mean value of survival rate index survey data of each factor impact and the significance of each influence factor effect of following table 3, table 4 through the multifactor analysis of variance of spss data processing software:
Table 3 different size cuttings cuttage survival rate application form
The single factor of table 4 is to each evaluation index contribution rate statistical form
The each target factor inspection of table 5
Dependent variable: 10 days numbers that sprout
A R Squared (R 2(Adjusted R Squared (adjusts R in)=0.126 2)=0.097)
By table 5 (several each target factor check tables that each factor carried out to variance analysis sprout for after cuttage 10 days), associative list 3, table 4, can find out:
1. diameter sprouts after 10 days on cuttage to count to affect and reaches utmost point significance level (P=0.001 < 0.01), and cuttings is more slightly more beneficial to and sprouts.
2. cuttings joint number is sprouted after 10 days on cuttage to count to affect and is not reached significance level (P=0.058 > 0.05).
3. cuttings has on-bladed cuttage was sprouted after 10 days number impact to reach utmost point significance level (P=0.007 < 0.01), and cuttings is beneficial to and sprouts without leaf.
4. diameter, joint number, have on-bladed three factors to reach significance level (P=0.024 < 0.05) for number impact that cuttage was sprouted after 10 days, so, carry out classification for cuttings and be necessary.
The each target factor inspection of table 6
Dependent variable: plant height after 10 days
A R Squared (R 2(Adjusted R Squared (adjusts R in)=0.491 2)=0.437)
By table 6 (each target factor check table that after cuttage, 10 days plant heights carry out variance analysis to each factor), associative list 3, table 4, can find out:
1. diameter on cuttage after 10 days plant height impact do not reach significance level (P=0.749 > 0.05).
2. cuttings joint number on cuttage after 10 days plant height impact reach utmost point significance level (P=0.005 < 0.01), cuttings joint number has certain influence to plant height.
3. cuttings have on-bladed on cuttage after 10 days plant height impact do not reach significance level (P=0.175 > 0.05).
4. diameter, joint number, have on-bladed three factors on cuttage after 10 days plant height impact reach utmost point significance level (P=0.000 < 0.01), so regulation cuttings joint number can have certain influence to later cuttage seeding plant height.
The each target factor inspection of table 7
Dependent variable: survival rate
A R Squared (R 2(Adjusted R Squared (adjusts R in)=0.300 2)=-0.030)
By table 7, final survival rate is carried out variance analysis each target factor check table to each factor, can find out:
On survival rate, impact does not all reach significance level to each factor.That is: cuttings specification is not remarkable on survival rate impact, only cuttage seeding specification is formed to impact.
The each target factor inspection of table 8
Dependent variable: radical
A R Squared (R 2(Adjusted R Squared (adjusts R in)=0.188 2)=0.144)
By table 8 (cuttage seeding radical carries out each target factor check table of variance analysis to each factor), associative list 3, table 4, can find out:
1. on cuttage seeding radical, impact does not reach utmost point significance level (P=0.000 > < 0.01) to diameter, and the thicker radical of cuttings is more.
2. on cuttage seeding radical, impact reaches utmost point significance level (P=0.006 < 0.01) to cuttings joint number, maximum with 4 root section numbers.
3. cuttings has on-bladed to affect and do not reach significance level (P=0.060 > 0.05) cuttage seeding radical, and cuttings has on-bladed not make significant difference to cuttage seeding radical.
4. diameter, joint number, have on-bladed three factors for cuttage seeding radical impact being reached to utmost point significance level (P=0.010=0.01), so, regulation cuttings specification can have certain influence to later cuttage seeding radical, directly affect the subsequent growth of transplanting survival rate and young plant, so early stage, young plant specification was necessary to carry out classification.
In sum, cuttings degree of lignification higher (diameter is thicker) more easily survives; Cuttings stays 4 joints best; Cuttings easily survives without leaf.
Embodiment 3:
Cuttings is processed the impact on cuttage test survival rate
Adopt Orthogonal Experiment and Design, investigate variety classes (clear water CK, indolebutyric acid IBA, root-inducing powder ABT), concentration and processing times 3 factor 3 impacts of horizontal growth conditioning agent on cuttage seedling.Each experimental factor, level and master-plan be in table 9, table 10:
The different growth regulator factor level of table 9 table
The different cuttings processing horizontal design of table 10 table
Because each processing has 30 repetitions, so data volume is huge, draw the mean value of the lower survival rate index survey data of each factor impact of following table 11, table 12 through the multifactor analysis of variance of spss data processing software:
Table 11 different disposal cuttings cuttage survival rate application form
The single factor of table 12 is to each evaluation index contribution rate statistical form
Data by table 11 and table 12 can be found out:
1. dip in root-inducing powder ABT and can improve survival rate, bring up to 78% by 67%.
2. dip in methyl α-naphthyl acetate NAA and root powder ABT can improve cuttage seeding radical, bring up to respectively 8.6 and 7.7 by 4.7.
3. the growth regulator of 100mg/L can promote sprouting and radical after cuttings cuttage.
4. the processing time of growth regulator is without significant difference, and institute thinks and saves time, and can dip in, dips in 20 seconds.
In sum, the growth regulator that cuttings is dipped in 100mg/L can promote sprouting and radical after cuttings cuttage for 20 seconds.
Embodiment 4:
On August 22nd, 2009, employing diameter is the cuttings of 0.5 centimetre, stay four joints, soak 12 hours with irrigation water, dip in the thiophanate methyl aqueous solution, then dip in the methyl α-naphthyl acetate state light root-inducing powder 20 seconds of 100 mg/litre, cuttage is in nursery, on March 22nd, 2010 lifting, root system dips in after thin mud, transplants in large Tanaka.
Embodiment 5:
On September 20th, 2009, employing diameter is the cuttings of 0.5 centimetre, stay four joints, soak 12 hours with irrigation water, dip in the thiophanate methyl aqueous solution, then dip in the methyl α-naphthyl acetate state light root-inducing powder 20 seconds of 100 mg/litre, cuttage is in nursery, on March 22nd, 2010 lifting, root system dips in after thin mud, transplants in large Tanaka.
Embodiment 6:
On October 10th, 2009, employing diameter is the cuttings of 0.5 centimetre, stay four joints, with irrigation water immersion 12 hours, dip in the thiophanate methyl aqueous solution, dip in again methyl α-naphthyl acetate state light root-inducing powder 20 seedlings of 100 mg/litre, cuttage is in nursery, then take hut lifting on March 22nd, 2010, root system is placed 48 hours after dipping in water conservation mud, transplants in large Tanaka, and wherein the dose volume ratio of moisturizing mud is particulate water-loss reducer (Yanggu Yongfa chemical plant, trade name is fruit tree water-loss reducer, a kind of super absorbent resin): clay: water=1: 150: 200.
Embodiment 7:
On November 10th, 2009, employing diameter is the cuttings of 0.5 centimetre, stay four joints, with irrigation water immersion 12 hours, dip in the thiophanate methyl aqueous solution, dip in again methyl α-naphthyl acetate state light root-inducing powder 20 seedlings of 100 mg/litre, cuttage is in nursery, then take hut lifting on March 22nd, 2010, root system is placed 48 hours after dipping in water conservation mud, transplants in large Tanaka, and wherein the dose volume ratio of moisturizing mud is particulate water-loss reducer (Yanggu Yongfa chemical plant, trade name is fruit tree water-loss reducer, a kind of super absorbent resin): clay: water=1: 160: 220.
Table 13 Various Seasonal survival rate statistical form
From table, survival rate data can be found out, above four seasons and operation all can be obtained by honeysuckle cuttings the honeysuckle cuttage seeding of high-quality, and then realize higher honeysuckle cuttage seeding transplanting survival rate.

Claims (5)

1. a cuttage and seedling culture method for honeysuckle, is characterized in that,
A, cuttage Season select: in the situation of cuttage in the open, cuttage is selected in eight season, the September; In the situation that taking hut, ten, the November carries out cuttage;
B, cuttings are selected: the necessary lignification of cuttings, and do not infect damage by disease and insect, stay 4 to 5 to save and remove all blades; Diameter >=0.5 centimetre of cuttings;
C, cuttings treatment conditions select: before cuttage, cuttings is dipped in the growth regulator 15 seconds to 1 minute of 50 to 150 mg/litre,
Described growth regulator is indolebutyric acid, root-inducing powder or methyl α-naphthyl acetate,
Cuttage seeding from nursery goes out garden to transplanting into taking following measures in the process of land for growing field crops:
For in-plant transplanting situation: honeysuckle nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time in 48 hours, in transportation, seedling root is dipped in to thin mud,
For remote transplanting situation: honeysuckle nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time exceedes 48 hours, or store temporarily, seedling root is dipped to the moisturizing mud that clay, water-loss reducer and water mix, the configuration proportion of moisturizing mud is: particulate water-loss reducer: clay: water=1: 120-160: 180-220, aforementioned proportion is volume ratio.
2. method according to claim 1, is further characterized in that, cuttings is stayed 4 joints.
3. method according to claim 1, is characterized in that,
In the situation that taking hut, ten, the November carries out cuttage;
Cuttings stays 4 to save and remove all blades; Diameter >=0.5 centimetre of cuttings;
Before cuttage, cuttings is dipped in the growth regulator 15 seconds to 30 seconds of 80 to 120 mg/litre, and described growth regulator is methyl α-naphthyl acetate.
4. method according to claim 1 and 2, is further characterized in that, the concentration of described growth regulator is 80 to 120 mg/litre.
5. method according to claim 1 and 2, is characterized in that, the time that cuttings is dipped in growth regulator is 15 seconds to 30 seconds.
CN201110042193.3A 2011-02-21 2011-02-21 Cuttage seedling culture method for honeysuckle CN102640631B (en)

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