CN112715576B - Seed coating agent for potatoes and preparation method thereof - Google Patents
Seed coating agent for potatoes and preparation method thereof Download PDFInfo
- Publication number
- CN112715576B CN112715576B CN202110026078.0A CN202110026078A CN112715576B CN 112715576 B CN112715576 B CN 112715576B CN 202110026078 A CN202110026078 A CN 202110026078A CN 112715576 B CN112715576 B CN 112715576B
- Authority
- CN
- China
- Prior art keywords
- parts
- seed coating
- coating agent
- liquid
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a potato seed coating agent and a preparation method thereof. The seed coating agent is prepared from raw materials consisting of compound microorganisms, rice water, urea, ferrous sulfate and clear water. The raw material components are 0.5 to 5.0 parts of compound microorganism, 10 to 20 parts of rice water, 0.1 to 0.5 part of urea, 0.10 to 0.20 part of ferrous sulfate and 75 to 90 parts of clear water according to weight. The preparation process of the seed coating agent comprises the following steps: mixing the single bacterial liquids according to a preset weight proportion to obtain a composite microorganism; mixing the raw material components according to a predetermined weight ratio to obtain a mixed material; performing facultative fermentation on the mixed material at the temperature of 30-38 ℃, then standing the mixture in an open manner, and finishing the fermentation when the pH value of the fermented material is less than or equal to 3.8; centrifuging the fermented liquid, and collecting supernatant as seed coating agent. The seed coating agent is used for planting potatoes, can promote the growth of the potatoes, and improves the yield and the quality of the potatoes.
Description
Technical Field
The invention belongs to the technical field of crop planting, and particularly relates to a potato seed coating agent and a preparation method thereof.
Background
With the development of our society, the consumption demand of people on grains is increasing day by day, but due to the influence of factors such as natural environment, cultivated land area, water resource and the like, the cost of continuous production increase of main grain varieties such as rice, corn, wheat and the like is increased, the lifting space is reduced, and the difficulty is increased. The potatoes have wide environmental adaptability and wide application range and are the fourth crop in the world. The potato planting method has important significance for food safety in China and sustainable development of society, but the acre yield of potatoes in China is low, and the consumption requirements of people cannot be met.
The seed coating technology is a comprehensive seed treating method which uses seeds as carriers and adopts a mechanical or manual method to uniformly coat the surface of the seeds with pesticides, bactericides, trace elements or plant growth agents so as to prevent and control diseases and pests at the seedling stage, promote plant growth and improve yield.
Therefore, the development of the potato seed coating agent containing beneficial microorganisms and the application of the potato seed coating agent to the planting of potatoes are significant to promoting the production of potatoes.
Disclosure of Invention
The invention aims to provide a potato seed coating agent and a preparation method thereof, which can promote the growth of potatoes and improve the yield of the potatoes.
On one hand, the invention provides a potato seed coating agent which is prepared from raw materials consisting of 0.5-5.0 parts of compound microorganism, 10-20 parts of rice water, 0.1-0.5 part of urea, 0.10-0.20 part of ferrous sulfate and 75-90 parts of clear water by weight, wherein the compound microorganism consists of lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid, the three bacterial liquids are 10-25 parts of lactobacillus plantarum bacterial liquid, 20-30 parts of saccharomyces cerevisiae bacterial liquid and 15-25 parts of bacillus subtilis bacterial liquid according to the proportioning relation by weight, and the bacterial content of the lactobacillus plantarum bacterial liquid is more than or equal to 3.0 multiplied by 1010cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterial content of the bacillus subtilis liquid is more than or equal to 5.0 multiplied by 109cfu/mL。
Preferably, the raw materials consist of 0.5 part of the compound microorganism, 13 parts of the rice water, 0.5 part of the urea, 0.16 part of the ferrous sulfate and 75 parts of the clean water by weight.
Preferably, the raw materials consist of 1.5 parts of the compound microorganism, 10 parts of the rice water, 0.4 part of the urea, 0.13 part of the ferrous sulfate and 80 parts of the clean water by weight.
Preferably, the raw materials consist of 3.5 parts of the compound microorganism, 17 parts of the rice water, 0.2 part of the urea, 0.10 part of the ferrous sulfate and 85 parts of the clean water by weight.
Preferably, the raw materials consist of 5.0 parts of the compound microorganism, 20 parts of the rice water, 0.1 part of the urea, 0.20 part of the ferrous sulfate and 90 parts of the clean water by weight.
On the other hand, the invention also provides a preparation method of the potato seed coating, which comprises the following steps:
step 1, mixing the single bacterial liquids according to a preset weight proportion to obtain the composite microorganism;
step 2, mixing the raw material components according to a preset weight ratio to obtain a mixed material;
step 3, performing facultative fermentation on the mixed material at the temperature of 30-38 ℃, then standing the mixture in an open manner, and finishing the fermentation when the pH value of the fermentation liquor is less than or equal to 3.8;
and 4, centrifuging the fermented liquid, collecting supernatant, and taking the supernatant as the potato seed coating agent.
Preferably, in step 3, the facultative fermentation time of the mixed materials is 72 hours, and the open standing time of the fermented materials is more than or equal to 5 days.
Preferably, in step 4, the centrifugation rotation speed of the fermentation liquid is 2000-4000r/min, and the centrifugation time is 10-30 min.
The potato seed coating agent is used for planting potatoes, can promote the growth of the potatoes, and improves the yield and the quality of the potatoes.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.
The seed coating agent for the potatoes is prepared by mixing compound microorganisms, rice water, urea, ferrous sulfate and clear water according to a certain proportion and then fermenting, wherein the rice water is thick liquid floating on the surface when rice is boiled. The potato seed coating agent is used for planting potatoes, can promote the growth of the potatoes, and improves the yield and the quality of the potatoes.
In one embodiment of the invention, the potato seed coating agent is prepared from raw materials consisting of compound microorganisms, rice water, urea, ferrous sulfate and clear water. The specific preparation process comprises the following steps: step 1, preparing a compound microorganism; step 2, preparing a mixed material, and step 3, fermenting the mixed material; and 4, centrifuging the fermentation liquor to obtain the seed coating agent.
In step 1 of preparing the complex microorganism, Lactobacillus plantarum (C)Lactobacillus plantarum) Bacterial liquid, Saccharomyces cerevisiae: (Saccharomyces cerevisiae) Bacterial liquid and Bacillus subtilisBacillus subtilis) And compounding the three kinds of bacteria liquid according to the weight ratio to obtain the composite microorganism, wherein 10-25 parts of lactobacillus plantarum bacteria liquid, 20-30 parts of saccharomyces cerevisiae bacteria liquid and 15-25 parts of bacillus subtilis liquid are preferred. The three kinds of bacteria liquid can be prepared by self, and lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media respectively to obtain the bacteria liquid. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus plantarum is not less than 3.0 × 1010cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 5.0 multiplied by 109cfu/mL。
In the step 2 of preparing the mixed material, the compound microorganism, the rice water, the urea, the ferrous sulfate and the clear water are weighed according to the preset weight proportion, and then the raw materials are added together, stirred and mixed uniformly to obtain the mixed material. The weight ratio of each raw material component is respectively optimized as follows: 0.5-5.0 parts of compound microorganism, 10-20 parts of rice water, 0.1-0.5 part of urea, 0.1-0.2 part of ferrous sulfate and 75-90 parts of clear water.
In the step 3 of fermenting the mixed material, the initial pH of the mixed material is natural, the mixed material is subjected to facultative fermentation at the temperature of 30-38 ℃, then the mixed material is left to stand, and the fermentation is finished when the pH of the fermentation liquid is less than or equal to 3.8. The fermentation time is preferably 72 hours, and the open standing time is preferably 5 days or more.
And 4, centrifuging the fermentation liquor to obtain the seed coating agent, centrifuging the liquid fermented in the step 3, and collecting the supernatant, wherein the obtained supernatant is the potato seed coating agent, the centrifugation speed is preferably 2000-4000r/min, and the centrifugation time is preferably 10-30 min.
When the potato seed coating agent is applied, the seed coating agent is diluted by 20-50 times with clear water, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds and continuously dried; or spraying diluted seed coating agent, throwing plant ash or talcum powder on the surface of the cut potato seeds, and then insolating for 2-4 hours and sowing. The dosage of the potato seed coating agent is preferably 0.1-0.5kg of the seed coating agent per 100kg of potatoes.
To assist in a better understanding of the technical solution of the present invention, the following examples are provided to illustrate the process of preparation and the method of application of the potato seed coating of the present invention.
Example one
The raw materials of the potato seed coating agent of the embodiment comprise, by weight, 0.5 part of compound microorganism, 13 parts of rice soup, 0.5 part of urea, 0.16 part of ferrous sulfate and 75 parts of clean water. The composite microorganism is prepared by compounding lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid, wherein the three bacterial liquids are 10 parts by weight of lactobacillus plantarum bacterial liquid, 23 parts by weight of saccharomyces cerevisiae bacterial liquid and 25 parts by weight of bacillus subtilis bacterial liquid. The three kinds of bacteria liquid are prepared by the inventor, and lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are respectively preparedThe bacillus is fermented and cultured in a corresponding liquid culture medium to obtain a bacterial liquid. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus plantarum is not less than 3.0 × 1010cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 5.0 multiplied by 109cfu/mL。
The potato seed coating agent is prepared by the following steps.
Step 1, preparing the composite microorganism, and uniformly mixing lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid to obtain the composite microorganism.
And 2, preparing mixed raw materials, adding the compound microorganisms, the rice water, the urea, the ferrous sulfate and clear water into a fermentation tank, stirring, and uniformly mixing to obtain a mixed material.
And 3, fermenting, wherein the initial pH of the mixed material is natural, carrying out facultative fermentation for 72 hours at the temperature of 30-38 ℃, then standing for 7 days in an open manner, and finishing the fermentation when the pH of the fermentation liquid is less than or equal to 3.6.
And 4, centrifuging the liquid fermented in the step 3 at 2000 r/min for 30min, and collecting supernatant to obtain the potato seed coating 1.
The application method of the potato seed coating agent 1 comprises the following steps: when planting potatoes, the seed coating agent 1 is diluted by 20 times by using clean water according to the amount of 0.1kg of seed coating agent applied to every 100kg of potatoes, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, and the potato seeds are continuously dried and sowed.
Example two
The raw materials of the potato seed coating agent of the embodiment comprise 1.5 parts of compound microorganism, 10 parts of rice soup, 0.4 part of urea, 0.13 part of ferrous sulfate and 80 parts of clear water by weight. The composite microorganism is prepared by compounding lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid, wherein the three bacterial liquids are 15 parts by weight of lactobacillus plantarum bacterial liquid, 26 parts by weight of saccharomyces cerevisiae bacterial liquid and 22 parts by weight of bacillus subtilis bacterial liquid. The three kinds of bacteria liquid are prepared by the inventor, lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media respectively to obtain the bacteria liquid. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus plantarum is not less than 3.0 × 1010cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 5.0 multiplied by 109cfu/mL。
The potato seed coating agent is prepared by the following steps.
Step 1, preparing the composite microorganism, and uniformly mixing lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid to obtain the composite microorganism.
And 2, preparing mixed raw materials, adding the compound microorganisms, the rice water, the urea, the ferrous sulfate and clear water into a fermentation tank, stirring, and uniformly mixing to obtain a mixed material.
And 3, fermenting, wherein the initial pH of the mixed material is natural, carrying out facultative fermentation for 72 hours at the temperature of 30-38 ℃, then standing for 5 days in an open manner, and finishing the fermentation when the pH of the fermentation liquid is less than or equal to 3.8.
And 4, centrifuging the liquid fermented in the step 3 for 25min at 2500 r/min, and collecting supernatant to obtain the potato seed coating 2.
The application method of the potato seed coating agent 2 comprises the following steps: when planting potatoes, the seed coating agent 2 is diluted by 30 times by using clean water according to the amount of 0.2kg of seed coating agent applied to every 100kg of potatoes, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, and the potato seeds are continuously dried and sowed.
EXAMPLE III
The raw materials of the potato seed coating agent of the embodiment comprise, by weight, 3.5 parts of compound microorganism, 17 parts of rice soup, 0.2 part of urea, 0.10 part of ferrous sulfate and 85 parts of clear water. The composite microorganism is prepared by compounding lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid, wherein the three bacterial liquids are respectively 20 parts by weight of lactobacillus plantarum bacterial liquid, 20 parts by weight of saccharomyces cerevisiae bacterial liquid and 17 parts by weight of bacillus subtilis bacterial liquid. The three kinds of bacterial liquids are prepared by the inventor, and lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media respectively to obtain the bacterial liquids. The bacterial contents of three single-strain bacterial liquids are respectively as follows: plant breast rodThe bacteria content is not less than 3.0 × 1010cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 5.0 multiplied by 109cfu/mL。
The potato seed coating agent is prepared by the following steps.
Step 1, preparing the composite microorganism, and uniformly mixing lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid to obtain the composite microorganism.
And 2, preparing mixed raw materials, adding the compound microorganisms, the rice water, the urea, the ferrous sulfate and clear water into a fermentation tank, stirring, and uniformly mixing to obtain a mixed material.
And 3, fermenting, wherein the initial pH of the mixed material is natural, carrying out facultative fermentation for 72 hours at the temperature of 30-38 ℃, then standing for 6 days in an open manner, and finishing the fermentation when the pH of the fermentation liquid is less than or equal to 3.5.
And 4, centrifuging the liquid fermented in the step 3 for 20min at 3000 r/min, and collecting supernatant to obtain the potato seed coating 3.
The application method of the potato seed coating agent 3 comprises the following steps: when planting potatoes, 0.3kg of seed coating agent is applied to every 100kg of potatoes, the seed coating agent 3 is diluted by 40 times by using clear water, then the diluted seed coating agent is sprayed on the surfaces of the cut potato seeds which are dried in the air, plant ash is thrown on the surfaces of the cut potato seeds, and the potatoes are sowed after being exposed for 2 hours.
Example four
The raw materials of the potato seed coating agent of the embodiment comprise 5.0 parts of compound microorganism, 20 parts of rice soup, 0.1 part of urea, 0.20 part of ferrous sulfate and 90 parts of clear water by weight. The composite microorganism is prepared by compounding lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid, wherein the three bacterial liquids are 25 parts by weight of lactobacillus plantarum bacterial liquid, 30 parts by weight of saccharomyces cerevisiae bacterial liquid and 15 parts by weight of bacillus subtilis bacterial liquid. The three kinds of bacterial liquids are prepared by the inventor, and lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are fermented and cultured in corresponding liquid culture media respectively to obtain the bacterial liquids. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus plantarum is not less than 3.0 × 1010cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterium content of the bacillus subtilis is more than or equal to 5.0 multiplied by 109cfu/mL。
The potato seed coating agent is prepared by the following steps.
Step 1, preparing the composite microorganism, and uniformly mixing lactobacillus plantarum bacterial liquid, saccharomyces cerevisiae bacterial liquid and bacillus subtilis bacterial liquid to obtain the composite microorganism.
And 2, preparing mixed raw materials, adding the compound microorganisms, the rice water, the urea, the ferrous sulfate and clear water into a fermentation tank, stirring, and uniformly mixing to obtain a mixed material.
And 3, fermenting, wherein the initial pH of the mixed material is natural, carrying out facultative fermentation for 72 hours at the temperature of 30-38 ℃, then standing for 8 days in an open manner, and finishing the fermentation when the pH of the fermentation liquid is less than or equal to 3.4.
And 4, centrifuging the liquid fermented in the step 3 for 10min at 4000r/min, and collecting supernatant to obtain the potato seed coating agent 4.
The application method of the potato seed coating agent 4 comprises the following steps: when planting potatoes, 0.5kg of seed coating agent is applied to every 100kg of potatoes, the seed coating agent 4 is diluted by 50 times by using clear water, then the diluted seed coating agent is sprayed on the surfaces of the cut potato seeds which are dried in the air, talcum powder is sprinkled on the surfaces of the cut potato seeds, and the potatoes are sowed after being exposed for 4 hours.
To help better understand the technical solution of the present invention, a test example of potato planting is provided below to illustrate the effect of the present invention.
Test example: application effect of potato seed coating agent in potato planting
The field to be tested is located in Qizihal city, Heilongjiang province, 5 groups of test designs are provided, each group of test designs 3 test cells, the area of each test cell is 20m2All test cells are randomly distributed.
Four test groups were each applied with potato seed coating 1-potato seed coating 4 prepared according to the present invention. The application mode of the potato seed coating agent 1 is as follows: according to the amount of 0.1kg of seed coating agent applied to every 100kg of potato seeds, the seed coating agent 1 is diluted by 20 times with clear water, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, and the potato seeds are continuously dried and sowed. The application mode of the potato seed coating 2 is as follows: according to the amount of 0.2kg of seed coating agent applied to every 100kg of potato seeds, the seed coating agent 2 is diluted by 30 times by using clean water, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, and the potato seeds are continuously dried and sowed. The application mode of the potato seed coating agent 3 is as follows: according to the amount of 0.3kg of seed coating agent applied to every 100kg of potato seeds, the seed coating agent 3 is diluted by 40 times by using clean water, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, plant ash is thrown to the surfaces of the potato seeds, and the potato seeds are sowed after being exposed for 2 hours. The application mode of the potato seed coating agent 4 is as follows: according to the amount of 0.5kg of seed coating agent applied to every 100kg of potato seeds, the seed coating agent 4 is diluted by 50 times by using clear water, then the diluted seed coating agent is sprayed on the surfaces of the dried potato seeds, the talcum powder is thrown to the surfaces of the potato seeds, and the potato seeds are sowed after being exposed for 4 hours. The control group was not coated with potato seed coating and the rest of the procedure was the same as the test group.
Selecting the 5 # Chinese potato as a test variety, sowing in late 4 months with the row spacing of 30cm and the row spacing of 65cm, and sowing 1 potato seed with 2-3 bud eyes in each hole. The same general management is adopted for each cell, and the cells are harvested in 9 middle-month.
And counting the number of seedlings in each cell during the sprouting period of the potatoes. In the bud stage of the potatoes, 10 potatoes are selected from each plot to measure the plant height and stem thickness of seedlings. After the potatoes are harvested, 15 plants are selected from each cell, the rotting rate of the potato blocks is measured, 5 potato blocks are randomly selected to measure the nitrate content in the potato blocks, and the yield of the potatoes in each cell is counted. The average rate of emergence, average plant height, average stem thickness, average potato block rot rate, average nitrate content in potato blocks and average plot potato yield were calculated for each group. The results are shown in Table 1.
TABLE 1
As can be seen from the data in Table 1, the rate of emergence, plant height, stem thickness and plot potato yield were significantly higher for the 4 groups of potatoes to which the potato seed coating was applied than for the control. Therefore, the prepared potato seed coating agent 1-the potato seed coating agent 4 can promote the growth of potatoes and improve the yield of the potatoes when being used for planting the potatoes. In addition, the data in Table 1 show that the rot rate of potato pieces and the nitrate content in potato pieces of 4 groups of potatoes applied with the potato seed coating agent are obviously lower than those of the control, which indicates that the potato quality can be obviously improved by applying the potato seed coating agent 1-the potato seed coating agent 4.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.
Claims (8)
1. A potato seed coating agent is characterized in that: the potato seed coating agent is prepared by raw materials consisting of 0.5-5.0 parts of compound microorganism, 10-20 parts of rice soup, 0.1-0.5 part of urea, 0.10-0.20 part of ferrous sulfate and 75-90 parts of clear water by weight,
the composite microorganism consists of lactobacillus plantarum liquid, saccharomyces cerevisiae liquid and bacillus subtilis liquid, wherein the three kinds of liquid are 10-25 parts by weight of the lactobacillus plantarum liquid, 20-30 parts by weight of the saccharomyces cerevisiae liquid and 15-25 parts by weight of the bacillus subtilis liquid, and the bacterial content of the lactobacillus plantarum liquid is more than or equal to 3.0 multiplied by 1010cfu/mL, the bacterium content of the saccharomyces cerevisiae liquid is more than or equal to 1.0 multiplied by 109cfu/mL, the bacterial content of the bacillus subtilis liquid is more than or equal to 5.0 multiplied by 109cfu/mL。
2. The potato seed coating of claim 1, wherein: the raw materials comprise, by weight, 0.5 part of the compound microorganism, 13 parts of the rice water, 0.5 part of the urea, 0.16 part of the ferrous sulfate and 75 parts of the clear water.
3. The potato seed coating of claim 1, wherein: the raw materials comprise, by weight, 1.5 parts of the compound microorganism, 10 parts of the rice water, 0.4 part of the urea, 0.13 part of the ferrous sulfate and 80 parts of the clear water.
4. The potato seed coating of claim 1, wherein: the raw materials comprise, by weight, 3.5 parts of the compound microorganism, 17 parts of the rice water, 0.2 part of the urea, 0.10 part of the ferrous sulfate and 85 parts of the clean water.
5. The potato seed coating of claim 1, wherein: the raw materials comprise, by weight, 5.0 parts of the compound microorganism, 20 parts of the rice water, 0.1 part of the urea, 0.20 part of the ferrous sulfate and 90 parts of the clean water.
6. A process for preparing a potato seed coating of any one of claims 1 to 5 comprising the steps of:
step 1, mixing the single bacterial liquids according to a preset weight proportion to obtain the composite microorganism;
step 2, mixing the raw material components according to a preset weight ratio to obtain a mixed material;
step 3, performing facultative fermentation on the mixed material at the temperature of 30-38 ℃, then standing the mixture in an open manner, and finishing the fermentation when the pH value of the fermentation liquor is less than or equal to 3.8;
and 4, centrifuging the fermented liquid, collecting supernatant, and taking the supernatant as the potato seed coating agent.
7. The method of claim 6, wherein: in the step 3, the facultative fermentation time of the mixed material is 72 hours, and the open standing time of the fermented material is more than or equal to 5 days.
8. The method of claim 6, wherein: in step 4, the centrifugal rotation speed of the fermentation liquid is 2000-4000r/min, and the centrifugal time is 10-30 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110026078.0A CN112715576B (en) | 2021-01-08 | 2021-01-08 | Seed coating agent for potatoes and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110026078.0A CN112715576B (en) | 2021-01-08 | 2021-01-08 | Seed coating agent for potatoes and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112715576A CN112715576A (en) | 2021-04-30 |
| CN112715576B true CN112715576B (en) | 2021-09-07 |
Family
ID=75591335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110026078.0A Active CN112715576B (en) | 2021-01-08 | 2021-01-08 | Seed coating agent for potatoes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112715576B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228052A (en) * | 2011-04-11 | 2011-11-02 | 贵州省生物技术研究所 | Potato minituber seed coating and preparation method thereof |
| CN103355357A (en) * | 2012-03-26 | 2013-10-23 | 上海万力华生物科技有限公司 | Potato seed coating |
| CN105237201A (en) * | 2015-10-13 | 2016-01-13 | 史莉娟 | Microbial agent compound corn seed coating agent |
| CN106045689A (en) * | 2016-06-02 | 2016-10-26 | 新昌县儒岙镇西雁山家庭农场 | Seed coating for preventing and controlling potato dry rot and preparation method thereof |
| CN107691455A (en) * | 2017-09-14 | 2018-02-16 | 商都县科技特派员工作站 | A kind of potato seed-coating agent |
| CN107915520A (en) * | 2017-11-29 | 2018-04-17 | 北京华夏源洁水务科技有限公司 | A kind of obducens seed and preparation method thereof |
| CN109220071A (en) * | 2018-09-29 | 2019-01-18 | 秦秋玲 | A kind of obducens seed and preparation method thereof |
| CN111548966A (en) * | 2020-05-22 | 2020-08-18 | 保罗蒂姆汉（潍坊）生物科技有限公司 | Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof |
| CN112154883A (en) * | 2020-09-18 | 2021-01-01 | 东北农业大学 | Planting method of potatoes in high-temperature environment |
| CN112154882A (en) * | 2020-09-18 | 2021-01-01 | 东北农业大学 | Green potato planting method suitable for secondary utilization of northern rice seedling raising shed |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103391710B (en) * | 2011-02-28 | 2015-04-29 | 罗地亚管理公司 | Seed coatings, coating compositions and methods for use |
-
2021
- 2021-01-08 CN CN202110026078.0A patent/CN112715576B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228052A (en) * | 2011-04-11 | 2011-11-02 | 贵州省生物技术研究所 | Potato minituber seed coating and preparation method thereof |
| CN103355357A (en) * | 2012-03-26 | 2013-10-23 | 上海万力华生物科技有限公司 | Potato seed coating |
| CN105237201A (en) * | 2015-10-13 | 2016-01-13 | 史莉娟 | Microbial agent compound corn seed coating agent |
| CN106045689A (en) * | 2016-06-02 | 2016-10-26 | 新昌县儒岙镇西雁山家庭农场 | Seed coating for preventing and controlling potato dry rot and preparation method thereof |
| CN107691455A (en) * | 2017-09-14 | 2018-02-16 | 商都县科技特派员工作站 | A kind of potato seed-coating agent |
| CN107915520A (en) * | 2017-11-29 | 2018-04-17 | 北京华夏源洁水务科技有限公司 | A kind of obducens seed and preparation method thereof |
| CN109220071A (en) * | 2018-09-29 | 2019-01-18 | 秦秋玲 | A kind of obducens seed and preparation method thereof |
| CN111548966A (en) * | 2020-05-22 | 2020-08-18 | 保罗蒂姆汉（潍坊）生物科技有限公司 | Microbial agent for promoting yield increase and disease resistance of potatoes and preparation method thereof |
| CN112154883A (en) * | 2020-09-18 | 2021-01-01 | 东北农业大学 | Planting method of potatoes in high-temperature environment |
| CN112154882A (en) * | 2020-09-18 | 2021-01-01 | 东北农业大学 | Green potato planting method suitable for secondary utilization of northern rice seedling raising shed |
Non-Patent Citations (3)
| Title |
|---|
| Seed Coating: A Tool for Delivering Beneficial Microbes to Agricultural Crops;Rocha Ines et al;《FRONTIERS IN PLANT SCIENCE 》;20191106;第10卷;第1357号 * |
| Using microbial seed coating for improving cowpea productivity under a low-input agricultural system;Rocha Ines et al;《JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 》;20191130;第100卷(第3期);第1092-1098页 * |
| 春种马铃薯种衣剂包衣处理试验;刘庸庆;《福建农业科技》;20130828(第8期);第50-51页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112715576A (en) | 2021-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105367335A (en) | Biochar-based coast saline-alkali land improvement conditioner and preparation method and application thereof | |
| CN112501052A (en) | Compound microorganism bacterium liquid for improving saline-alkali soil and application thereof | |
| CN112500220A (en) | Liquid compound microorganism foliar fertilizer for improving saline-alkali soil and application thereof | |
| CN112501072B (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN112500232A (en) | Fish protein leaf surface bio-organic fertilizer and application thereof | |
| CN101805716B (en) | Antagonistic bacterium for controlling bacterial wilt of capsicum and microorganism organic fertilizer thereof | |
| CN112794750A (en) | Foliar fertilizer for increasing potato yield and preparation method thereof | |
| CN110004082A (en) | A kind of bacterium bacterial strain and application suitable for the regulation of industrialized agriculture nitrogen and phosphorus pollution | |
| CN112205274A (en) | Rice seedling raising matrix based on various agricultural wastes and preparation method thereof | |
| CN112616857A (en) | Liquid compound microorganism rooting agent for improving saline-alkali soil and application thereof | |
| CN112661579A (en) | Phosphate solubilizing microbial preparation capable of improving saline-alkali soil and preparation method thereof | |
| CN112500216A (en) | Bio-organic fertilizer with crop rooting promoting function and preparation method thereof | |
| CN110606782A (en) | Farmland soil water-retaining organic fertilizer and preparation method thereof | |
| CN112299914A (en) | Preparation method of special biological slow-release fertilizer for rice | |
| CN112655719A (en) | Microbial preparation for promoting rice rooting and preparation method and application thereof | |
| CN110537478B (en) | Camellia oleifera seedling raising substrate and preparation method and seedling raising method thereof | |
| CN102816028B (en) | Biological disease prevention type greenhouse vegetable planting fertilizer and preparation method and application thereof | |
| CN112479788A (en) | Solid-state composite microorganism water flush fertilizer for improving saline-alkali soil and application thereof | |
| CN112715576B (en) | Seed coating agent for potatoes and preparation method thereof | |
| CN112586511B (en) | Seed dressing agent for promoting germination of crop seeds planted in saline-alkali soil and seed treatment method | |
| AU2021105906A4 (en) | Yield-increase cultivation medium of continuous-cropping peanuts and yield-increase cultivation method thereof | |
| CN113233936A (en) | Preparation method of leaf fertilizer for promoting crop rooting | |
| CN115280926A (en) | Saline-alkali soil improvement and restoration method | |
| CN113248317A (en) | Preparation method of leaf fertilizer for promoting rice maturity and improving rice yield | |
| CN113372154A (en) | Foliar fertilizer rich in selenium and calcium and application method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |