CN103355357A - Potato seed coating - Google Patents
Potato seed coating Download PDFInfo
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- CN103355357A CN103355357A CN2012100813703A CN201210081370A CN103355357A CN 103355357 A CN103355357 A CN 103355357A CN 2012100813703 A CN2012100813703 A CN 2012100813703A CN 201210081370 A CN201210081370 A CN 201210081370A CN 103355357 A CN103355357 A CN 103355357A
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Abstract
The invention provides a novel application of trichoderma sp. The invention provides a potato seed coating and a method for preventing and treating ring rot of potato and also changes the condition that trichoderma sp is mainly used for preventing and treating diseases caused by plant pathogenic fungi in the past. The potato seed coating taking trichoderma sp as an active component has high prevention efficiency which is over 60%.
Description
Technical field
The present invention relates to a kind of biopesticide, relate in particular to a kind of kind clothing agent that can effectively prevent and treat potato bacterial ring rot.
Background technology
Ring rot is the larger disease of harm in the potato planting production process, generation is all arranged throughout the country, it causes that the overground part cauline leaf is dispirited, the underground part stem tuber rots along bundle ring generation ring-type, its Field diseases on average is about 21.6%, the light field underproduction 13% of falling ill, and the serious underproduction is more than 60%, and cause duration of storage potato piece large quantities of rotten, cause huge economic loss every year.
Potato bacterial ring rot claims again to take turns maize ear rot, be commonly called as " it is mashed to turn-take ", a kind of bacillary vascular bundle diseases, through the withered or appearance explosion of storage tuberogemma blackening, after sick potato is sowed, part eye rots not germinate, or it is rear withered or form diseased plant to sprout, the sick bud that a part is unearthed, and germ rises to the stem middle part or enters new knot potato piece and cause a disease along stem along vascular bundle, the root of diseased plant, the normal browning of stem's vascular bundle, the sick climing white bacterial ooze that sometimes overflows.This disease not only affects output, and mashed cellar for storing things affects tuber quality when also causing storage.
Potato bacterial ring rot belong to the rotten excellent bacillus of pathogenetic bacteria ring (the rotten mutation of causing a disease of Michigan rod bacillus potato ring,
Clavibacter michiganenseSubsp.
Sepedonicum(Spieckermann ﹠amp; Kotthoff) Davis) in classification, belong to corynebacterium in the bacterium.Encircle rotten excellent bacillus and survive the winter in potato seed, become the primary source of infection in next year, germ also can survive for a long time holding on the container of potato seed, becomes the source that the potato piece infects.Encircle rotten excellent bacillus and be difficult to long-time survival in soil, the route of transmission mainly is when cutting the potato piece, and germ can be infected 24 ~ 28 strong potatos by the cutting knife infection of carrying disease germs according to the sick potato of test partial application.Although germ is only infected potato under field conditions (factors), in the artificial infection situation, also can infect more than 30 and plant plant of Solanaceae.Especially the potato seed sexual involution is fast, needs in a lot of situations to keep production by transferring to plant, and causes ring rot to be easy to spread to other area.
Adopt whole photo sowing can reduce the propagation that encircles rotten excellent bacillus, but in actual seeding process, the potato seed amount that the method needs is excessive; Prior to seeding, potato seed is dried in the air kinds 5 ~ 6 days, reject mashed potato during this period, also can reduce the generation of field ring rot, but can't retrieve the loss that a large amount of mashed potatos have caused; Calcium phosphate can strengthen the resistance against diseases of potato, but can not fundamentally solve ring rot.The seed selection new varieties are the another approach that solve disease, but before selecting the new varieties that can popularize plantation, still are urgent problems to the control of ring rot.
Agricultural chemicals seed soaking or seed dressing are the effective means of killing pathogenic bacteria, such as copper sulphate, fenaminosulf (claiming again fenaminosulf), topsin (comprising thiophanate methyl, ethyl topsin), kasugarnycin copper oxychloride etc., potato bacterial ring rot all there is certain control efficiency.But above-mentioned chemical pesticide is residual very serious, and soil is polluted, and also human health is brought harm simultaneously.
Summary of the invention
Lack or the residual serious problem of chemical pesticide for potato bacterial ring rot control product, the invention provides a kind of kind clothing agent be used to preventing and treating potato bacterial ring rot.
First aspect of the present invention provides a kind of potato seed-coating agent be used to preventing and treating ring rot, and its active component comprises Trichoderma.
Described Trichoderma is preferably viable spore, can be the various forms such as viable spore zymotic fluid or viable spore powder; Among the present invention, be preferably the viable spore zymotic fluid.
Of the present invention kind of clothing agent can be the available formulations such as suspending agent, pastel or paste, wetting powder, solution.
According to a kind of preferred embodiment of of the present invention kind of clothing agent, described kind of clothing agent is solution or suspending agent, and the weight fraction of Trichoderma viable spore zymotic fluid is 1.5 ~ 10%, more preferably 1.5 ~ 5%, and such as 2%, 3%.
Another kind of preferred embodiment according to of the present invention kind of clothing agent also comprises humectant and sticker.
Wherein, described humectant as required, can be the hydrophily humectant, such as glycerine, glyceride, polyethylene glycol, sorbierite, xylitol, propane diols, butanediol, shitosan, pyrrolidone sodium carboxylate etc.; Also can be the lipophile humectant, such as vaseline, lanolin, octadecanol etc.; The perhaps any combination of above-mentioned substance.
Wherein, described sticker comprises: any one in starch, starch derivatives, cellulose, cellulose derivatives, colloid or polysaccharide or the synthetic high polymer sticker or multiple mixture; Such as methylcellulose, ethyl cellulose, microcrystalline cellulose, CMC, hydroxypropyl cellulose, hydroxypropyl methylcellulose, hydroxyethylcellulose, HEMC, hydroxymethyl-propyl cellulose, hydracrylic acid methylcellulose or its salt, starch, sodium carboxymethyl starch, gum Arabic, carragheen, gelatin, agar, algin, xanthans, guar gum, locust bean gum, konjac glucomannan, pectin, polyethylene acetate fiber (comprising its salt or ester), polyethylene glycol oxide, polyvinyl alcohol, polyvinylidene chloride (PVDC); The perhaps any combination of above-mentioned substance.
Wherein, the weight ratio of Trichoderma viable spore zymotic fluid and humectant and sticker is preferably 1 ~ 10:1 ~ 10:1 ~ 5, more preferably 1 ~ 5:1 ~ 5:1 ~ 3.
In above-mentioned any clothing agent of the present invention, can also comprise other auxiliary agents such as acceptable solvent, surfactant, filler, disintegrant, preservative, antifreezing agent, bleeding agent, dyestuff, warning colouration in the agricultural chemicals.
One skilled in the art will appreciate that in any above-mentioned clothing agent of the present invention, can also comprise in the agricultural chemicals acceptable for the drug component of other disease of control or little fertilizer and/or the plant growth regulator of promotion growth.
Second aspect of the present invention provides the application of a kind of Trichoderma in preventing and treating potato bacterial ring rot, and Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries, sows.
The 3rd aspect of the present invention provides a kind of method of preventing and treating potato bacterial ring rot, and Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries, sows.
The 4th aspect of the present invention provides the potato seed processing method before a kind of potato sowing, and Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries.
In second, third that the present invention is above-mentioned or the 4th aspect, described Trichoderma is preferably Trichoderma viable spore zymotic fluid.
A kind of preferred embodiment of second, third or the 4th aspect according to the present invention, wherein, the percentage by weight that described Trichoderma viable spore zymotic fluid accounts for described solution or suspension is preferably 1.5 ~ 10%, and more preferably 1.5 ~ 5%.
A kind of preferred embodiment of second, third or the 4th aspect according to the present invention, wherein, the time of soaking potato seed is preferably 1 ~ 5min, more preferably 2 ± 0.5min.
A kind of preferred embodiment of second, third or the 4th aspect wherein, can also comprise humectant and sticker in described solution or the suspension according to the present invention.
Wherein, described humectant as required, can be the hydrophily humectant, such as glycerine, glyceride, polyethylene glycol, sorbierite, xylitol, propane diols, butanediol, shitosan, pyrrolidone sodium carboxylate etc.; Also can be the lipophile humectant, such as vaseline, lanolin, octadecanol etc.; The perhaps any combination of above-mentioned substance.
Wherein, described sticker comprises: any one in starch, starch derivatives, cellulose, cellulose derivatives, colloid or polysaccharide or the synthetic high polymer sticker or multiple mixture; Such as methylcellulose, ethyl cellulose, microcrystalline cellulose, CMC, hydroxypropyl cellulose, hydroxypropyl methylcellulose, hydroxyethylcellulose, HEMC, hydroxymethyl-propyl cellulose, hydracrylic acid methylcellulose or its salt, starch, sodium carboxymethyl starch, gum Arabic, carragheen, gelatin, agar, algin, xanthans, guar gum, locust bean gum, konjac glucomannan, pectin, polyethylene acetate fiber (comprising its salt and ester), polyethylene glycol oxide, polyvinyl alcohol, polyvinylidene chloride (PVDC).
Wherein, the weight ratio of Trichoderma viable spore zymotic fluid and humectant and sticker is preferably 1 ~ 10:1 ~ 10:1 ~ 5, more preferably 1 ~ 5:1 ~ 5:1 ~ 3.
In the solution or suspension that the present invention is above-mentioned, can also comprise other auxiliary agents such as acceptable solvent, surfactant, filler, disintegrant, preservative, antifreezing agent, bleeding agent, dyestuff, warning colouration in the agricultural chemicals.
In the above-mentioned content of the present invention, the preparation of Trichoderma viable spore zymotic fluid the Chinese patent of applicant's previous application (application number: be described 201110044517.7), the content in the described patent, can be complete be referred in the content of the present invention.
One skilled in the art will appreciate that in the solution or suspension that the present invention is above-mentioned, can also comprise in the agricultural chemicals acceptable for the drug component of other disease of control or little fertilizer and/or the plant growth regulator of promotion growth.Before this, Trichoderma is used to prevent and treat the disease that plant pathogenic fungi causes substantially, and the present invention provides a kind of new application for Trichoderma.
The invention provides a kind of kind clothing agent be used to preventing and treating potato bacterial ring rot, and the method for preventing and treating potato bacterial ring rot, potato bacterial ring rot is had good control efficiency.
Embodiment
The invention provides a kind of potato seed-coating agent, as active component, simultaneously the present invention also provides a kind of method of potato bacterial ring rot and processing method of seed potato of preventing and treating with Trichoderma.
Below by specific embodiment the present invention is described in detail and describes, so that understand better content of the present invention, but should be understood that following embodiment does not limit the scope of the invention.
Among the following embodiment of the present invention, the preparation of described Trichoderma viable spore zymotic fluid, adopting application number is method in 201110044517.7 the Chinese invention patent, what the content in this patent can be complete is referred among the present invention; But also can adopt existing other fermentation technique in this area.Described wood is mould can be that Trichoderma viride, healthy and free from worry wood are mould, Trichoderma harzianum, trichoderma asperellum, long shoot wood are mould etc.
Embodiment 1
Trichoderma viable spore zymotic fluid, glycerine, methylcellulose mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 10% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 2
Add pathogen in the potato seed.
Trichoderma viable spore zymotic fluid, glycerine, methylcellulose mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 2% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 3
Trichoderma viable spore zymotic fluid, glycerine, methylcellulose mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 1.5% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 4
Trichoderma viable spore zymotic fluid, glycerine, methylcellulose mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 1% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 5
Trichoderma viable spore zymotic fluid, glycerine, methylcellulose mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 5% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Preventive effect the results are shown in Table 1 among the embodiment 1 ~ 5.
Table 1, embodiment 1 ~ 4 prevents and treats the potato bacterial ring rot effect
| Embodiment | Blank | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Average root long (cm) | 19.19 | 20.5 | 19.88 | 19.50 | 17.94 | 20.13 |
| Average root heavy (g) | 5.69 | 6.36 | 7.57 | 6.83 | 4.65 | 6.76 |
| Average plant height (cm) | 25.13 | 15.44 | 18.91 | 16.44 | 14.31 | 16.51 |
| Average strain heavy (g) | 8.56 | 5.38 | 8.40 | 7.56 | 5.23 | 6.92 |
| Average stem thick (mm) | 9.05 | 10.10 | 11.29 | 10.55 | 10.55 | 10.74 |
| Disease refers to | 34.38 | 10.89 | 12.50 | 13.11 | 17.70 | 11.57 |
| Preventive effect | - | 68.32% | 63.64% | 61.02% | 48.50% | 65.59% |
Embodiment 6
Trichoderma viable spore zymotic fluid, glycerine, CMC mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 2% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 7
Add pathogen in the potato seed.
Trichoderma viable spore zymotic fluid, glycerine, sodium carboxymethyl starch mix according to the weight ratio of 2:2:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 2% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 8
Add pathogen in the potato seed.
Trichoderma viable spore zymotic fluid, glycerine, carragheen mix according to the weight ratio of 5:5:3, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 5% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 9
Add pathogen in the potato seed.
Trichoderma viable spore zymotic fluid, glycerine, polyethylene glycol oxide mix according to the weight ratio of 5:3:1, add clear water, are mixed with Trichoderma viable spore zymotic fluid concentration and are 7% solution, after stirring, soak about 2 minutes of potato seed, potato seed are dried sowing.
All are processed potato seed plant in seedling-growing container, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.All preliminary treatment potato seeds are seeded into respectively seedling-growing container, the pathogen that occurs mashed kind is added in the nutrient matrix, regularly water, observe the rotten situation of seed potato growing state and potato seed stem tuber etc.
Embodiment 6 ~ 9 preventive effects the results are shown in Table 2.
Table 2, embodiment 5 ~ 8 prevents and treats the potato bacterial ring rot effect
| Embodiment | Blank | Embodiment 6 | Embodiment 7 | Embodiment 8 | Embodiment 9 |
| Preventive effect | - | 61.00% | 58.55% | 65.12% | 67.32% |
More than specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (10)
1. one kind is used for preventing and treating the potato seed-coating agent of ring rot, it is characterized in that described kind of clothing agent active component comprises Trichoderma.
2. according to claim a kind of clothing agent is characterized in that, described Trichoderma is the viable spore zymotic fluid.
3. according to claim 2 kind of clothing agent is characterized in that, described kind of clothing agent is solution or suspending agent, and wherein, the weight fraction of Trichoderma viable spore zymotic fluid is 1.5 ~ 10%.
4. according to claim a kind of clothing agent is characterized in that, described kind of clothing agent also comprises humectant and sticker.
5. the application of Trichoderma in preventing and treating potato bacterial ring rot is characterized in that, Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries, sows.
6. a method that prevents potato bacterial ring rot is characterized in that, Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries, sows.
7. method according to claim 6 is characterized in that, described Trichoderma is Trichoderma viable spore zymotic fluid.
8. method according to claim 7 is characterized in that, the percentage by weight that described Trichoderma viable spore zymotic fluid accounts for described solution or suspension is 1.5 ~ 10%.
9. the potato seed processing method that potato sowing is front is characterized in that, Trichoderma is prepared into solution or suspension, then soaks potato seed, then dries.
10. method according to claim 9 is characterized in that, described Trichoderma is Trichoderma viable spore zymotic fluid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210081370.3A CN103355357B (en) | 2012-03-26 | 2012-03-26 | A kind of potato seed-coating agent |
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| CN201210081370.3A CN103355357B (en) | 2012-03-26 | 2012-03-26 | A kind of potato seed-coating agent |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489932A (en) * | 2016-10-17 | 2017-03-15 | 上海万力华生物科技有限公司 | A kind of trichoderma oil-suspending agent and preparation method thereof |
| CN106818951A (en) * | 2017-03-17 | 2017-06-13 | 南宁市健佳网络科技有限公司 | It is a kind of to prevent and treat bactericide of bacterial ring rot o potato and preparation method thereof |
| CN110199811A (en) * | 2019-07-03 | 2019-09-06 | 云南省农业科学院经济作物研究所 | A kind of implantation methods promoting late autumn in winter High Yield Potato |
| CN112715576A (en) * | 2021-01-08 | 2021-04-30 | 东北农业大学 | Seed coating agent for potatoes and preparation method thereof |
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| CN1718000A (en) * | 2005-08-11 | 2006-01-11 | 上海交通大学 | Composite coating agent for corn, and prepn. method therefor |
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| CN1718000A (en) * | 2005-08-11 | 2006-01-11 | 上海交通大学 | Composite coating agent for corn, and prepn. method therefor |
| CN1911043A (en) * | 2006-08-10 | 2007-02-14 | 上海交通大学 | Method for preparing biological type seed coating agent for anti-cucumber powdery mildew of system induction type |
| CN101491258A (en) * | 2008-01-25 | 2009-07-29 | 李树春 | Pure traditional Chinese medicine potato seed-coating agent |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489932A (en) * | 2016-10-17 | 2017-03-15 | 上海万力华生物科技有限公司 | A kind of trichoderma oil-suspending agent and preparation method thereof |
| CN106818951A (en) * | 2017-03-17 | 2017-06-13 | 南宁市健佳网络科技有限公司 | It is a kind of to prevent and treat bactericide of bacterial ring rot o potato and preparation method thereof |
| CN110199811A (en) * | 2019-07-03 | 2019-09-06 | 云南省农业科学院经济作物研究所 | A kind of implantation methods promoting late autumn in winter High Yield Potato |
| CN112715576A (en) * | 2021-01-08 | 2021-04-30 | 东北农业大学 | Seed coating agent for potatoes and preparation method thereof |
| CN112715576B (en) * | 2021-01-08 | 2021-09-07 | 东北农业大学 | Seed coating agent for potatoes and preparation method thereof |
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| CN103355357B (en) | 2016-05-11 |
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