CN114766490B - New application of brassinolide and method for improving water conservation function of grassland - Google Patents
New application of brassinolide and method for improving water conservation function of grassland Download PDFInfo
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- CN114766490B CN114766490B CN202210482140.1A CN202210482140A CN114766490B CN 114766490 B CN114766490 B CN 114766490B CN 202210482140 A CN202210482140 A CN 202210482140A CN 114766490 B CN114766490 B CN 114766490B
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 46
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 title claims abstract description 44
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 19
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 31
- 241000196324 Embryophyta Species 0.000 claims abstract description 19
- 150000001647 brassinosteroids Chemical class 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 238000005507 spraying Methods 0.000 claims description 25
- 230000002708 enhancing effect Effects 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000004480 active ingredient Substances 0.000 claims 1
- 230000006870 function Effects 0.000 description 23
- 241000209140 Triticum Species 0.000 description 9
- 235000021307 Triticum Nutrition 0.000 description 9
- 239000002028 Biomass Substances 0.000 description 8
- 244000025221 Humulus lupulus Species 0.000 description 8
- 235000008694 Humulus lupulus Nutrition 0.000 description 8
- 238000009331 sowing Methods 0.000 description 8
- -1 oleuropein lactone Chemical class 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000006872 improvement Effects 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- RFWGABANNQMHMZ-UHFFFAOYSA-N 8-acetoxy-7-acetyl-6,7,7a,8-tetrahydro-5H-benzo[g][1,3]dioxolo[4',5':4,5]benzo[1,2,3-de]quinoline Natural products CC=C1C(CC(=O)OCCC=2C=C(O)C(O)=CC=2)C(C(=O)OC)=COC1OC1OC(CO)C(O)C(O)C1O RFWGABANNQMHMZ-UHFFFAOYSA-N 0.000 description 2
- HKVGJQVJNQRJPO-UHFFFAOYSA-N Demethyloleuropein Natural products O1C=C(C(O)=O)C(CC(=O)OCCC=2C=C(O)C(O)=CC=2)C(=CC)C1OC1OC(CO)C(O)C(O)C1O HKVGJQVJNQRJPO-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- RFWGABANNQMHMZ-CARRXEGNSA-N oleuropein Natural products COC(=O)C1=CO[C@@H](O[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O)C(=CC)[C@H]1CC(=O)OCCc3ccc(O)c(O)c3 RFWGABANNQMHMZ-CARRXEGNSA-N 0.000 description 2
- 235000011576 oleuropein Nutrition 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241000132542 Conyza Species 0.000 description 1
- 241001580986 Elymus tangutorum Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- RFWGABANNQMHMZ-HYYSZPHDSA-N Oleuropein Chemical compound O([C@@H]1OC=C([C@H](C1=CC)CC(=O)OCCC=1C=C(O)C(O)=CC=1)C(=O)OC)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O RFWGABANNQMHMZ-HYYSZPHDSA-N 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention relates to the technical field of plant planting, in particular to a novel application of brassinolide and a method for improving a grassland water source conservation function thereof. The invention provides application of brassinolide as a preparation for improving the water conservation function of grassland. The novel brassinosteroids can be used for improving the water conservation function, particularly the water conservation function of the artificial grass of the Meibina, further expanding the application range of brassinolide and further expanding the application range of the artificial grass of the Meibina.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to a novel application of brassinolide and a method for improving a grassland water source conservation function thereof.
Background
The Mebinia (Elymus tangutorum Hand-Mzza.) is a wild grass widely distributed in high-altitude areas, is nontoxic, has high plants, has strong cold resistance and barren resistance, and is poor in palatability, and most researchers consider that the Mebinia can be used as ecological grass seed development research, but the research shows that the underground biomass of the Mebinia is lower than pasture of other grass genera such as old mango and conyza, so that the water source conservation function of the artificial grassland is poor. However, the poor water conservation function of the artificial grass of the Meibina can affect the recovery or reconstruction effect of the artificial grass of the Meibina on the degraded grass, so that one of the key problems of the water conservation function of the artificial grass of the Meibina is enhanced. The prior art generally obtains the Mebinia chinensis variety capable of improving the water conservation function through the traditional and transgenic breeding means, however, the mode takes too long and takes effect too slowly.
In view of this, the present invention has been made.
Disclosure of Invention
The invention aims to provide a new application of brassinolide and a method for improving the water conservation function of grassland. The embodiment of the invention provides a novel application of brassinolide, which can obviously improve the water conservation function of the artificial grass of the Mebine.
The invention is realized in the following way:
in a first aspect, the present invention provides the use of brassinolide as a formulation for enhancing the water conservation function of grassland.
In an alternative embodiment, the use includes enhancing the water conservation function of the artificial grass of Mebinia.
In a second aspect, the present invention provides a method of enhancing the water conservation function of a lawn, comprising: spraying brassinolide on leaf surface.
In an alternative embodiment, the method comprises: and when the seeds emerge for one month after sowing, the brassinolide is sprayed on leaf surfaces.
In an alternative embodiment, the method further comprises: and when the plants are planted for one month after the next year of turning green, the brassinolide is sprayed on the leaf surfaces of the plants.
In an alternative embodiment, the concentration of brassinosteroids sprayed is 3.5-4.5nM, preferably 4nM.
In an alternative embodiment, the brassinolide is sprayed in an amount of 0.8 to 1.2L/m 2 Preferably 1L/m 2 。
In an alternative embodiment, the crop that promotes water conservation includes a Mebinia artificial grass.
In a third aspect, the present invention provides a method for planting a wheat bine artificial grassland, comprising: spraying brassinolide.
In an alternative embodiment, the method comprises: spraying brassinolide onto leaf surfaces when seedlings emerge for one month after sowing seeds in the first year, and spraying brassinolide onto leaf surfaces of plants when planting for one month after turning green in the second year, wherein the concentration of the sprayed brassinolide is 3.5-4.5nM, and the spraying amount of the brassinolide is 0.8-1.2L/m 2 。
The invention has the following beneficial effects: the embodiment of the invention provides a new application of brassinolide, which can be used for improving the water conservation function, in particular to the water conservation function of the artificial grass of the Mebinia, further expands the application range of the brassinolide and also further expands the application range of the artificial grass of the Mebinia.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Brassinolide (BR) is a natural compound with biological activity and takes sterols as a basic structure, is a novel plant hormone, can play an important role in regulating and controlling the growth and development of plants like other five major plant hormones (auxin, gibberellin, ethylene, abscisic acid and cytokinin), and is known as a sixth major hormone. Specifically, brassinolide is also called brassinolide, is widely used in pollen, seeds, stems, leaves and other organs of plants, has extremely low content in the plants, has extremely high physiological activity, and can show obvious physiological effect of promoting growth after being treated with extremely low concentration. However, it is also only described in the prior art that brassinosteroids can be used to promote plant growth, but there is no description or report on other uses of brassinosteroids, in particular for improving water conservation.
The embodiment of the invention provides application of brassinolide as a preparation for improving the water conservation function of grasslands, in particular to improvement of the water conservation function of the artificial grasslands of the Meibina.
The invention provides a new application of brassinolide, further expands the cognition of researchers on brassinolide, further expands the application range of brassinolide, and brings a opportunity for agricultural production and development, in particular to a new leap of restoration or reconstruction of degraded grasslands.
The embodiment of the invention also provides a method for improving the water conservation function of the grassland, which comprises the following steps: spraying brassinolide on leaf surface.
Specifically, after the seeds are sowed in the first year and one month of emergence, brassinosteroids are sprayed on the leaf surfaces of the seedlings, and the brassinosteroids act on the seedlings, so that the field water holding capacity and the underground biomass can be improved, and then the water conservation function is improved.
The seeding method is described in the prior art, and the embodiments of the present invention will not be described in detail.
More preferably, the brassinosteroids are sprayed on the leaf surfaces of the plants when the plants are planted until one month after the next year of turning green. That is to say, the brassinolide is continuously sprayed in the next year, so that the field water holding capacity and underground biomass can be further improved, and the water conservation function is further improved.
Further, the concentration of the brassinolide to be sprayed is any value between 3.5 and 4.5nM, for example, 3.5nM, 3.6nM, 3.7nM, 3.8nM, 3.9nM, 4.0nM, 4.1nM, 4.2nM, 4.3nM, 4.4nM and 4.5nM, preferably 4nM. The concentration can be beneficial to improving the effect of the brassinosteroids on improving the water conservation function.
Further, the spraying amount of brassinolide is 0.8-1.2L/m 2 For example, 0.8L/m 2 、0.9L/m 2 、1.0L/m 2 、1.1L/m 2 1.2L/m 2 0.8-1.2L/m 2 Any number in between, preferably 1L/m 2 。
Further, crops that promote water conservation include the artificial grass of Mebinia.
By adopting the mode to treat the plants, the water source conservation function of the plants can be effectively improved, the survival probability of the plants is improved, and the improvement of the plants to the environment is also facilitated.
In a third aspect, the present invention provides a method for planting a wheat bine artificial grassland, comprising: spraying brassinolide onto leaf surfaces when seedlings emerge for one month after sowing seeds in the first year, and spraying brassinolide onto leaf surfaces of plants when planting for one month after turning green in the second year, wherein the concentration of the sprayed brassinolide is 3.5-4.5nM, and the spraying amount of the brassinolide is 0.8-1.2L/m 2 。
The method for planting the artificial grass of the Mebinia can increase the underground biomass and the field water holding capacity of the Mebinia, can further remarkably improve the water conservation function of the artificial grass of the Mebinia, further improve the recovery and reconstruction effects of the artificial grass of the Mebinia on the degraded grass, and enlarge the application range of the artificial grass of the Mebinia.
The features and capabilities of the present invention are described in further detail below in connection with the examples.
Example 1
The present embodiment provides a pot test of the Mebinia, specifically,
pot experiments were performed in green sea university greenhouse in the western city. The specification of the planting pot is 8-8 cm, and the planting matrix is sterilized nutrient soil and vermiculite 1:1, planting single plants, spraying different concentrations of the oleuropein lactone solutions (0.1, 0.2, 1, 4 and 8 nM) on leaf surfaces for one week after the emergence of the wheat bine seedlings, spraying 20ml every two days, repeating each treatment concentration for 5 times, washing out root systems after three times of spraying, and measuring relevant parameters of the wheat bine root systems, wherein specific results are shown in table 1.
TABLE 1
As is clear from Table 1, when the application concentration of brassinolide was 4nM, the parameters related to root system such as root length, root surface area and root reference were optimal, indicating that the optimal application concentration of brassinolide was 4nM.
Example 2
The present implementation provides a field cell test of the order of magnitude of the wheat, specifically,
the field cell test was performed in the county of dari, fruit, county of nest, race, county of dar, fruit. The test cell area was 3 x 5m, each handling 3 duplicate cells. The processing of each cell is basically the same, specifically: the sowing is carried out for manual soil preparation, fertilization (diamine phosphate, fertilization amount is 20 Kg/mu), deep turning and fine harrowing. Ditching and drill sowing after soil preparation, sowing depth of 1cm, row spacing of 20cm, sowing amount of 1 Kg/mu, earthing after sowing and compacting after 0.5 cm. The difference is that the post-emergence treatment is that, in particular, the experimental group 1, the leaf surface is sprayed with 4nM of the olein lactone solution when the seedling emerges for one month, the application amount is 1L/m 2 The method comprises the steps of carrying out a first treatment on the surface of the Experimental group 2-spraying no olein lactone solution one month after seedling emergence, spraying pure water with the same amount, and experimental group 3-spraying 4nM olein lactone solution on leaf surface one month after seedling emergence with the application amount of 1L/m 2 4nM oleurolactone solution is sprayed on the leaf surface again 1 month after the next year of turning green, the application amount is 1L/m 2 Experimental group 4-leaf surface spraying 4nM oleuropein solution when seedling emerges for one month, the application amount is 1L/m 2 The olein lactone solution is not sprayed 1 month after the next year of turning green, and the pure water with the same amount is sprayed.
The cells were then tested for the water conservation function of the Mebine artificial grassland water source, see specifically Table 2.
TABLE 2
As can be seen from Table 2, in the first year of planting, the subsurface biomass of the brassinolide-sprayed maibine artificial grass was increased by 49.4% relative to the subsurface biomass of the brassinolide-not-sprayed maibine artificial grass, and the field water holding capacity of the brassinolide-sprayed maibine artificial grass was increased by 18.9% relative to the field water holding capacity of the brassinolide-not-sprayed maibine artificial grass. In the second year of planting, the underground biomass of the wheat bine grass artificial grassland still spraying brassinosteroids in the second year is increased by 47.9% compared with that of the wheat bine grass artificial grassland not spraying brassinosteroids in the first year, and the field water holding capacity of the wheat bine grass artificial grassland still spraying brassinosteroids in the second year is increased by 13.3% compared with that of the wheat bine grass artificial grassland not spraying brassinosteroids in the second year. The spraying of brassinolide can obviously improve the underground biomass and the field water holding capacity of the artificial grass land of the Meibina, and the brassinolide can obviously improve the water conservation function of the artificial grass land of the Meibina.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A method for enhancing the conservation function of a water source in a mobium grassland, comprising: spraying brassinolide on leaf surfaces after the seed of the Mebine grass is sown and seedlings emerge for one month, wherein: the concentration of the sprayed brassinosteroids is 3.5-4.5. 4.5nM, and the spraying amount of the brassinosteroids is 0.8-1.2L/m 2 。
2. The method for enhancing a water conservation function of a mobium grassland according to claim 1, further comprising: and when the plants are planted for one month after the next year of turning green, the brassinolide is sprayed on the leaf surfaces of the plants.
3. The method for improving the water conservation function of the mobium grassland according to any one of claims 1 to 2, wherein the concentration of the brassinosteroids sprayed is 4nM.
4. The method for improving the conservation function of a water source of a Mebine grassland according to any one of claims 1 to 2,characterized in that the spraying amount of brassinolide is 1L/m 2 。
5. The use of brassinolide as sole active ingredient in the preparation of a formulation for enhancing the water conservation function of a Meibina artificial turf.
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CN108293661A (en) * | 2018-02-11 | 2018-07-20 | 中国科学院植物研究所 | A method of improving natural meadow high quality forage ratio and biological yield |
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AU2019223390A1 (en) * | 2018-02-26 | 2020-10-29 | Anish Mahendrabhai AMIN | An additive for soil conditioning and an agricultural composition containing said additive for plant growth |
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CN106721146A (en) * | 2016-11-29 | 2017-05-31 | 中国科学院植物研究所 | A kind of sheep's hay accelerator and its application |
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