CN112474789A - Method for promoting growth of anthurium andraeanum under heavy metal stress - Google Patents
Method for promoting growth of anthurium andraeanum under heavy metal stress Download PDFInfo
- Publication number
- CN112474789A CN112474789A CN202011142280.1A CN202011142280A CN112474789A CN 112474789 A CN112474789 A CN 112474789A CN 202011142280 A CN202011142280 A CN 202011142280A CN 112474789 A CN112474789 A CN 112474789A
- Authority
- CN
- China
- Prior art keywords
- heavy metal
- soil sample
- growth
- stress
- anthurium andraeanum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
Abstract
The invention discloses a method for promoting growth of anthurium andraeanum under heavy metal stress, which comprises the following steps: respectively extracting a normal soil sample, a lead-zinc ore heavy metal slightly-polluted soil sample and a lead-zinc ore heavy metal heavily-polluted soil sample to obtain leaching liquor, and applying exogenous sodium silicate to the leaching liquor; selecting small white palm plants with good growth vigor and close biomass; culturing the berberis poiretii with the three leaching solutions, culturing in an environment with scattered light at a windowsill, performing aeration treatment with an aerator, and covering each treated culture pot with black plastic. By the method, the damage of heavy metal to the anthurium andraeanum can be effectively relieved, and the growth of the anthurium andraeanum under the stress of the lead-zinc ore heavy metal can be promoted.
Description
Technical Field
The invention relates to the technical field of plant heavy metal stress growth research, in particular to a method for promoting growth of anthurium andraeanum under heavy metal stress.
Background
Heavy metal pollution of soil is a serious environmental problem in China and even the world. In 2014, the national soil pollution survey report is issued by the ministry of environmental protection and the ministry of national union of national soil resources, and the result shows that the national soil environment condition is not optimistic and the pollution of part of regions is serious. The total overproof rate of the soil pollutants in the whole country is 16.1 percent, and cadmium is one of the main pollutants. The investigation result of the Ministry of agriculture also shows that the irrigation area of the pollutants in China is about 140 ten thousand hm2, and the land area polluted by heavy metals accounts for 64.8 percent of the total polluted area. The main heavy metal pollution elements in the soil include elements such as lead, zinc, cadmium, mercury and the like, and in recent years, the content of the heavy metal elements in the soil tends to increase gradually.
The anthurium andraeanum belongs to a plant of Araceae, is named as the taro (Spathiphyllum kochii Engl. & K.Krause), and has ornamental value to the taro and the bract taro (Oryza sativa L.). Its origin is in the tropical regions of the americas, and it is now commonly cultivated around the world. It is a perennial herb with very short, large or no stems, generally growing to around 30-40 cm. As the anthurium andraeanum flower has beautiful leaves, is light and rich in posture and grows vigorously, is deeply favored by people, has good beautifying and environment repairing effects when being used as a garden plant to be planted in a heavy metal polluted area, and has good prospects in the technical field of large-scale plant planting in the development and utilization of heavy metal polluted mining areas.
However, the research on beautifying and repairing the environment by applying garden plants to a heavy metal polluted area is rare at present, the research on applying the anthurium andraeanum to the ecological pollution of the heavy metal is very rare, the yield is improved by improving the stress resistance of the anthurium andraeanum to the heavy metal or relieving the stress effect of the heavy metal on the growth of the anthurium andraeanum, and the research on the method for promoting the growth of the anthurium andraeanum under the stress of the heavy metal is very important.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for promoting the growth of anthurium andraeanum under the stress of heavy metal. By applying the exogenous sodium silicate, the growth of the anthurium andraeanum can be effectively promoted to resist the stress of heavy metal lead.
In order to achieve the purpose, the invention adopts the following technical scheme:
the method for promoting the growth of the anthurium andraeanum under the stress of heavy metal comprises the following steps:
(1) respectively grinding a normal soil sample, a lead-zinc ore heavy metal slightly-polluted soil sample and a lead-zinc ore heavy metal heavily-polluted soil sample, sieving by a 10-mesh sieve, respectively weighing 5kg of the ground soil sample, respectively extracting leaching liquor by deionized water, and applying external sodium silicate to the leaching liquor;
(2) selecting small white palm plants with good growth vigor and biomass and plant height close to each other, cleaning the small white palm plants with tap water, and rinsing the small white palm plants with deionized water for three times;
(3) culturing the berberis poiretii with the three leaching solutions, culturing in an environment with scattered light at windowsill, treating with aerator for oxygen supply, and coating black plastic bag on each treated culture basin.
Preferably, when the deionized water is used for extracting the leaching liquor in the step (1), 5L of deionized water and 5kg of soil sample are mixed and stirred for 0.5-1 h, then the mixture is vibrated for 2h at 200r/min by using an oscillator, the standing is carried out for 0.5h, the supernatant is filtered by using analysis filter paper to obtain the leaching liquor, 2L of the leaching liquor is divided into two equal parts, wherein sodium silicate is added into one part, and the concentration of the sodium silicate is 1 mM.
Preferably, the biomass of the spatholobus suberectus in the step (2) is 36.5-37.5 g, and the plant height is 32-33 cm.
Preferably, when the aerator supplies oxygen for the treatment in the step (3), the aerator continuously supplies oxygen for twenty-four hours.
In summary, the invention has the following advantages:
the exogenous sodium silicate is added to carry out water culture treatment on the berberis pointei, so that the toxic action of heavy metals can be effectively relieved, the growth of the berberis pointei is promoted, the relieving action is obvious under the severe pollution stress of the heavy metals in lead-zinc ores, the biomass is improved by 16.05% compared with that of the sodium silicate which is not added, the net photosynthesis is improved by 13.2%, the contents of chlorophyll a and chlorophyll b are respectively improved by 41% and 21%, the APX activity is improved by 71.6%, the POD activity is improved by 32.8%, the CAT activity is improved by 7.3%, and the MDA content is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic illustration of the effect on biomass of sodium silicate application;
FIG. 2 is a schematic diagram showing the effect of sodium silicate application on the net photosynthetic rate of plants;
FIG. 3 shows the contents of chlorophyll a and chlorophyll b in plants after sodium silicate is applied;
FIG. 4 is a schematic representation of the effect of sodium silicate application on plant MDA;
FIG. 5 is a schematic representation of the effect of sodium silicate application on POD activity in plants;
FIG. 6 is a schematic diagram showing the effect of sodium silicate application on CAT activity in plants;
FIG. 7 is a schematic diagram showing the effect of sodium silicate application on the APX activity content of plants;
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for promoting growth of anthurium andraeanum under heavy metal stress comprises the following steps:
(1) preparation of sodium silicate solution
Accurately weighing 1.2206g of anhydrous sodium silicate by using a ten thousand-point electronic balance in an aseptic environment, pouring the anhydrous sodium silicate into a beaker filled with deionized water and sterilized in advance, uniformly stirring, transferring the beaker into a 100ml capacity bottle, and fixing the volume of the beaker to 100ml by using the deionized water to prepare 100mM sodium silicate mother liquor for later use.
(2) Preparation of lead-zinc ore leaching liquor slightly polluted by heavy metal
Weighing 5kg of lead-zinc ore soil sample which is crushed in advance and sieved by a 10-mesh sieve and lightly polluted by heavy metal in a sterile environment, evenly distributing the soil sample to 5 plastic bottles which are sterilized in advance and filled with 1L of deionized water, mixing and stirring for 0.5h, then shaking for 2h by using an oscillator, standing for 0.5h, filtering supernatant by using analysis filter paper to obtain leaching liquor with the water-soil ratio of 1: 1, mixing and stirring the leaching liquor obtained by filtering in 5 bottles uniformly, taking out 1.99L, and distributing the leaching liquor into two culture boxes, wherein one culture box is 0.99L of leaching liquor, and the other culture box is 1L of leaching liquor.
(3) Sodium silicate applied in leaching liquor
And (3) taking 10ml of the sodium silicate mother liquor obtained in the step (1) into a culture box with 0.99L of leaching liquor by using a pipette under an aseptic environment, diluting the sodium silicate concentration by one hundred times, namely 1mM, and finally controlling the solution amount in the two culture boxes to be consistent, wherein the solution amount is 1L.
(4) Culture of Anthurium albiflora
Selecting 6 small spatholobus plants with good growth vigor and approximate biomass and plant height, cleaning the plants with tap water, rinsing the plants with deionized water for three times, wherein the biomass and the plant height are 36.5-37.5 g and 32-33 cm, placing the small spatholobus into a culture box pool for culture, placing 3 plants in each pot, culturing in an environment with scattered light at a windowsill, carrying out oxygen supply treatment by an aerator, and coating a black plastic bag outside each treated culture pot. On 5 th day of cultivation, leaching solutions in all treatment basins were replaced by leaching solutions prepared in the same way as before and sodium silicate, and on 10 th day net photosynthesis was measured, followed by harvesting and measurement of biomass, APX, POD, CAT, MDA and chlorophyll a and chlorophyll b.
Example 2
Different from the embodiment 1, the method for promoting the growth of the anthurium andraeanum under the stress of the heavy metal comprises the following steps:
(1) preparation of sodium silicate solution
Accurately weighing 1.2206g of anhydrous sodium silicate by using a ten thousand-point electronic balance in a sterile environment, pouring the anhydrous sodium silicate into a beaker filled with deionized water and sterilized in advance, uniformly stirring, transferring the beaker into a 100ml volumetric flask, and fixing the volume of the beaker to 100ml by using the deionized water to prepare 100mM sodium silicate mother liquor for later use.
(2) Preparation of leaching liquor of lead-zinc ore heavily polluted by heavy metal
Weighing 5kg of heavy metal heavily polluted lead-zinc ore soil sample which is crushed in advance and sieved by a 10-mesh sieve in a sterile environment, evenly distributing the heavy metal heavily polluted lead-zinc ore soil sample to 5 plastic bottles which are sterilized in advance and are filled with 1L of deionized water, mixing and stirring for 0.5h, then shaking for 2h by using an oscillator, standing for 0.5h, filtering supernatant by using analysis filter paper to obtain leaching liquor with the water-soil ratio of 1: 1, mixing and stirring the leaching liquor obtained by filtering in 5 bottles uniformly, taking out 1.99L, and distributing the leaching liquor into two culture boxes, wherein one culture box is used for 0.99L of leaching liquor, and the other culture box is used for 1L of leaching liquor.
(3) Sodium silicate applied in leaching liquor
And (3) taking 10ml of the sodium silicate mother liquor obtained in the step (1) into a culture box with 0.99L of leaching liquor by using a pipette under an aseptic environment, diluting the sodium silicate concentration by one hundred times, namely 1mM, and finally controlling the solution amount in the two culture boxes to be consistent, wherein the solution amount is 1L.
(4) Culture of Anthurium albiflora
Selecting 6 small spatholobus plants with good growth vigor and approximate biomass and plant height, cleaning the plants with tap water, rinsing the plants with deionized water for three times, wherein the biomass and the plant height are 36.5-37.5 g and 32-33 cm, placing the small spatholobus into a culture box pool for culture, placing 3 plants in each pot, culturing in an environment with scattered light at a windowsill, carrying out oxygen supply treatment by an aerator, and coating a black plastic bag outside each treated culture pot. On 5 th day of cultivation, leaching solutions in all treatment basins were replaced by leaching solutions prepared in the same way as before and sodium silicate, and on 10 th day net photosynthesis was measured, followed by harvesting and measurement of biomass, APX, POD, CAT, MDA and chlorophyll a and chlorophyll b.
Comparative example
Different from the embodiment 1, the method for promoting the growth of the anthurium andraeanum under the stress of the heavy metal comprises the following steps:
the same quality of normal uncontaminated soil sample was used in place of the lead-zinc ore soil sample slightly contaminated with heavy metal in example 1, and after the leaching solution was prepared, only 1L of the leaching solution was taken out to a culture pot without adding sodium silicate, thereby forming a control group (CK).
TABLE 1 treatment of various soil extracts and application of sodium silicate
Analysis of results
As can be seen from figures 1-7, the application of sodium silicate can effectively relieve the toxic action of heavy metals in lead-zinc ores and promote the growth of berberis albus, wherein the relieving action is obvious under the severe pollution stress of the heavy metals in the lead-zinc ores, the biomass is improved by 16.05% by adding exogenous sodium silicate compared with the biomass without adding sodium silicate, the net photosynthesis is improved by 13.2%, the contents of chlorophyll a and chlorophyll b are respectively improved by 41% and 21%, the APX activity is improved by 71.6%, the POD activity is improved by 32.8%, the CAT activity is improved by 7.3%, and the MDA content is reduced by 43%.
While the present invention has been described in detail with reference to the illustrated embodiments, it should not be construed as limited to the scope of the present patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.
Claims (4)
1. A method for promoting the growth of anthurium andraeanum under the stress of heavy metals, which is characterized by comprising the following steps:
(1) respectively grinding a normal soil sample, a lead-zinc ore heavy metal slightly-polluted soil sample and a lead-zinc ore heavy metal heavily-polluted soil sample, sieving by a 10-mesh sieve, respectively weighing 5kg of the ground soil sample, respectively extracting leaching liquor by deionized water, and applying external sodium silicate to the leaching liquor;
(2) selecting small white palm plants with good growth vigor and biomass and plant height close to each other, cleaning the small white palm plants by using tap water, and rinsing the small white palm plants by using deionized water for three times;
(3) culturing the berberis poiretii with the three leaching solutions, culturing in an environment with scattered light at windowsill, treating with aerator for oxygen supply, and coating black plastic bag on each treated culture pot.
2. The method for promoting the growth of anthurium andraeanum under the stress of heavy metals according to claim 1, wherein in the step (1), when the leaching solution is extracted by deionized water, 5L of deionized water and 5kg of soil sample are mixed and stirred for 0.5-1 h, then the mixture is shaken for 2h at 200r/min by using an oscillator, the standing is carried out for 0.5h, the supernatant is filtered by using analytical filter paper to obtain the leaching solution, 2L of the leaching solution is divided into two equal parts, wherein sodium silicate is added into one part, and the concentration of the sodium silicate is 1 mM.
3. The method for promoting the growth of anthurium andraeanum under the stress of heavy metals according to claim 1, wherein the biomass of the anthurium andraeanum in the step (2) is 36.5-37.5 g, and the plant height is 32-33 cm.
4. The method for promoting the growth of anthurium andraeanum under the stress of heavy metals according to claim 1, wherein the aeration machine continuously aerates for 24 hours when the aeration machine supplies oxygen for treatment in the step (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011142280.1A CN112474789A (en) | 2020-10-14 | 2020-10-14 | Method for promoting growth of anthurium andraeanum under heavy metal stress |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011142280.1A CN112474789A (en) | 2020-10-14 | 2020-10-14 | Method for promoting growth of anthurium andraeanum under heavy metal stress |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112474789A true CN112474789A (en) | 2021-03-12 |
Family
ID=74926159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011142280.1A Pending CN112474789A (en) | 2020-10-14 | 2020-10-14 | Method for promoting growth of anthurium andraeanum under heavy metal stress |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112474789A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113661885A (en) * | 2021-06-04 | 2021-11-19 | 广西师范大学 | Method for relieving poisoning of lawn pennywort herb caused by cadmium stress |
| CN114080954A (en) * | 2021-10-15 | 2022-02-25 | 广西师范大学 | Method for relieving stress of multiple metals on pennywort herb |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106734161A (en) * | 2016-12-22 | 2017-05-31 | 环保桥（湖南）生态环境修复有限公司 | A kind of APP combined remediation methods for heavy metal pollution reparation of ploughing |
-
2020
- 2020-10-14 CN CN202011142280.1A patent/CN112474789A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106734161A (en) * | 2016-12-22 | 2017-05-31 | 环保桥（湖南）生态环境修复有限公司 | A kind of APP combined remediation methods for heavy metal pollution reparation of ploughing |
Non-Patent Citations (4)
| Title |
|---|
| 敖雪桔等: "《环境保护概论》", 31 December 2008, 哈尔滨地图出版社 * |
| 李天哲等: "镉胁迫下硅对水稻幼苗生长与生理响应的影响", 《农业环境科学学报》 * |
| 蔡庆生: "《植物生理学》", 31 August 2014, 中国农业大学出版社 * |
| 贺永清: "《家庭花经》", 31 August 2003, 上海文化出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113661885A (en) * | 2021-06-04 | 2021-11-19 | 广西师范大学 | Method for relieving poisoning of lawn pennywort herb caused by cadmium stress |
| CN114080954A (en) * | 2021-10-15 | 2022-02-25 | 广西师范大学 | Method for relieving stress of multiple metals on pennywort herb |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103521508B (en) | Phytoremediation method for soil with cadmium pollution | |
| CN106386445A (en) | Rooting method of Hylotelephium erythrostictum by using water culture and cutting | |
| CN112474789A (en) | Method for promoting growth of anthurium andraeanum under heavy metal stress | |
| CN109511500B (en) | Biological method for reducing cadmium content of rice in cadmium-polluted rice field | |
| CN102175554B (en) | Method for screening variety with highest nitrogen use ratio from various cut-flower chrysanthemum varieties | |
| CN107971334A (en) | A kind of method of more pools-algae water cyclic irrigation system repairing polluted soil | |
| CN107125016A (en) | A kind of greenhouse method for massively culturing of orchid | |
| CN103828509A (en) | Rapid and effective soil improvement method | |
| CN102668853B (en) | Blueberry twig plant cuttage root-taking cultivation method | |
| CN106694545A (en) | Method for restoring heavy metal Cu-contaminated soil | |
| CN104478556A (en) | Special foliar spray agent for tobacco and preparation method and using method of spray agent | |
| CN104607453A (en) | Reinforced plant restoration method for lead-cadmium composite contaminated soil | |
| CN101596541B (en) | Method for remedying heavy metals in domestic waste leakage solution | |
| CN105330485B (en) | A kind of barrier crops absorb foliar fertilizer and its application of cadmium | |
| CN105850535A (en) | Method for improving salt stress tolerance of medicago truncatula | |
| CN106345795A (en) | Method for promoting symphytum officinale to enrich and absorb heavy metal from heavy metal contaminated soil | |
| CN108739003A (en) | A kind of plant maintaining method of Liana rosa indica | |
| CN108191566A (en) | A kind of gold Rosa roxburghii Tratt orchard soil improvement bio-fertilizer and soil improvement method | |
| CN108085015A (en) | Bed mud heavy metal deactivator and preparation method thereof | |
| CN104355880A (en) | Special medium for high-grade seedling cuttage | |
| CN107159691A (en) | A kind of method of biological synergetic repairing heavy metal polluted soil of farmland | |
| CN103586268A (en) | Application of lantana camara to remediation of heavy metal zinc, lead and copper polluted soil | |
| CN103875398B (en) | A kind of method that reduces chromium content in tobacco leaf | |
| CN102989758A (en) | Method for repairing lead-polychlorinated biphenyl combined polluted soil | |
| CN102523787A (en) | Culture method for increasing number and activity of alfalfa root nodules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210312 |
|
| WD01 | Invention patent application deemed withdrawn after publication |