CN102523787A - Culture method for increasing number and activity of alfalfa root nodules - Google Patents
Culture method for increasing number and activity of alfalfa root nodules Download PDFInfo
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- CN102523787A CN102523787A CN2012100247842A CN201210024784A CN102523787A CN 102523787 A CN102523787 A CN 102523787A CN 2012100247842 A CN2012100247842 A CN 2012100247842A CN 201210024784 A CN201210024784 A CN 201210024784A CN 102523787 A CN102523787 A CN 102523787A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a culture method for increasing the number and activity of alfalfa root nodules. The culture method includes the following steps: after being disinfected, alfalfa seeds are placed into a culture dish with a piece of filter paper, and are germinated for 3 to 4 days in an illuminating incubator, the seedlings are then transplanted into a culture bowl filled up with quartz sand, rhizobia are inoculated during transplantation, nutrient solution is poured in the culture bowl, rhizobia are inoculated once again when the seedlings are grown to about 10cm, the nitrogen concentration of the nutrient solution is then decreased to less than 5mM, the nutrient solution is poured for one day, and water is poured for another day. The culture method can greatly increase the activity of alfalfa root nodules and the number of rhizobia and sufficiently exert the effect of root nodules in the growth of alfalfa, thus increasing the yield of alfalfa and improving the quality of alfalfa.
Description
Technical field
The invention belongs to agriculture field, in particular to a kind of alfalfa Phylloxera quantity and active cultural method of improving.
Background technology
Alfalfa (Medicago sativa L.) has another name called clover, lucerne, have the laudatory title of " king of herbage ", be to cultivate the earliest, distribute the widest in the world, and also be most excellent, most important a kind of high-quality legume forage.Alfalfa is at the existing cultivation history more than 2000 of China, and all there is plantation all parts of the country, but mainly are distributed in northwest and North China.The hat of in the majority kind of leguminous forage of alfalfa hay yield, and nutrient component is complete, and rich in proteins, trace element and multivitamin have the potentiality that are developed to health products, food additives and biological agent raw materials for production.Because have the flourishing root system and the characteristic of biological nitrogen fixation, alfalfa still is a kind of good soil melioration and water and soil conservation plant, establishing in large scale can improve soil nutrient, prevent the salinization of soil and desertification.How many activity of rhizobium have crucial influence to the production of clover with.At present, conventional alfalfa Phylloxera cultural method is an earth culture, and this kind method can be turned out root nodule, but the root nodule dross number of being cultivated is few, and is active low, thereby reduced the advantage of root nodule in alfalfa growing, influenced the yield and quality of clover thereupon.
Summary of the invention
The present invention seeks to increases rhizobium quantity in order to improve the activity of alfalfa Phylloxera, gives full play to the effect of root nodule in alfalfa growing, thereby increases alfalfa output, improves alfalfa quality.In order to realize the object of the invention, intend and adopt following technical scheme:
One aspect of the present invention relates to a kind of alfalfa Phylloxera quantity and active cultural method of improving, and it comprises the steps: to place the culture dish that is covered with filter paper with after the alfalfa seed sterilization; In illumination box, germinate, after waiting to germinate 3-4 days, transplant to the culturing pot that fills up quartz sand; When transplanting, carry out the inoculation of rhizobium, water with nutrient solution, growth of seedling carries out a legume inoculation during to the 10cm left and right sides again; Then, the nitrogen concentration that reduces nutrient solution carries out the alternately property pouring of one day nutrient solution one sky and water to 5mM; Described nutrient solution is formed by major constituents and microcomponent configuration, and described major constituents comprises Ca (NO
3)
2, KCl, KH
2PO
4, KNO
3, K
2SO
4, MgSO
4And NH
4H
2PO
4Described microcomponent comprises H
3BO
3, CuSO
4, MnSO
4, (NH
4)
6Mo
7O
24, ZnSO
4, FeSO
4And EDTA.
In a preferred implementation of the present invention, the proportioning of described major constituents is 4723-5723: 956-1256: 676-976: 1516-2016: 1233-1533: 4314-5314: 1437-2037.
In a preferred implementation of the present invention, the proportioning of described microcomponent is 4.24-6.24: 0.20-0.40: 3.80-5.80: 0.43-0.63: 0.49-0.70: 5570-7570: 7450-9450.
The present invention can increase substantially the activity of alfalfa Phylloxera, increases rhizobium quantity, gives full play to the effect of root nodule in alfalfa growing, thereby increases alfalfa output, improves alfalfa quality.
Embodiment:
In order to further specify technical scheme of the present invention, adopt following concrete scheme to cultivate alfalfa Phylloxera, it comprises the steps:
(5% clorox soaked 5 minutes with the alfalfa seed sterilization; 70% alcohol-pickled 10 seconds) after; Place the culture dish (100 seeds of each culture dish) that is covered with filter paper, (25 ℃ of light, dark 25 ℃ of 12h/, 12h) germinates in illumination box, during note keeping the skin wet.After waiting to germinate 3-4 days, transplant to the culturing pot that fills up quartz sand.When transplanting, carry out the inoculation of rhizobium.After this, water with full nitrogen nutrition liquid (table 1).
Table 1 nutrient solution prescription
Carry out a legume inoculation during to growth of seedling to the 10cm left and right sides again.Then, the nitrogen concentration of nutrient solution is reduced to below the 5mM/L, carries out the alternately property pouring of one day nutrient solution one sky and water.So continue, in the time of 50 days, just can obtain the rhizobium that pink has nitrogenase activity.Repeat above experiment three times, detect the output of rhizobium etc., the result is as shown in table 2.
Table 2 rhizobium cultivation results
| Method | Dross number/strain | Output g/m 2 | Average growth rate cm/ days |
| Conventional method | 0～200 | 4511 | 1.07 |
| The embodiment method | 300～500 | 5089 | 1.47 |
When being understood that, above-mentioned embodiment only is an illustrative, concerning those of ordinary skills, can improve or conversion according to above-mentioned explanation, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention.
Claims (4)
1. the cultural method of an alfalfa Phylloxera, it comprises the steps: to place the culture dish that is covered with filter paper with after the alfalfa seed sterilization; In illumination box, germinate, after waiting to germinate 3-4 days, transplant to the culturing pot that fills up quartz sand; When transplanting, carry out the inoculation of rhizobium, water with nutrient solution, growth of seedling carries out a legume inoculation during to the 10cm left and right sides again; Then, the nitrogen concentration that reduces nutrient solution carries out the alternately property pouring of one day nutrient solution one sky and water to 5mM; Described nutrient solution is formed by major constituents and microcomponent configuration, and described major constituents comprises Ca (NO
3)
2, KCl, KH
2PO
4, KNO
3, K
2SO
4, MgSO
4And NH
4H
2PO
4Described microcomponent comprises H
3BO
3, CuSO
4, MnSO
4, (NH
4)
6Mo
7O
24, ZnSO
4, FeSO
4And EDTA.
2. the cultural method of a kind of alfalfa Phylloxera according to claim 1, the proportioning of described major constituents is 4723-5723: 956-1256: 676-976: 1516-2016: 1233-1533: 4314-5314: 1437-2037.
3. alfalfa Phylloxera cultural method according to claim 1 and 2, the proportioning of described microcomponent are 4.24-6.24: 0.20-0.40: 3.80-5.80: 0.43-0.63: 0.49-0.70: 5570-7570: 7450-9450.
4. any described culture fluid among the claim 1-3.
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| CN201210024784.2A CN102523787B (en) | 2012-02-06 | 2012-02-06 | Culture method for increasing number and activity of alfalfa root nodules |
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| CN201210024784.2A CN102523787B (en) | 2012-02-06 | 2012-02-06 | Culture method for increasing number and activity of alfalfa root nodules |
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| CN102523787A true CN102523787A (en) | 2012-07-04 |
| CN102523787B CN102523787B (en) | 2014-04-16 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105144911A (en) * | 2015-09-28 | 2015-12-16 | 江苏农林职业技术学院 | Method for cultivating grass-like plant |
| CN105660108A (en) * | 2016-01-08 | 2016-06-15 | 山西省农业科学院高寒区作物研究所 | High-yield cultivation method for alfalfa in semi-arid region |
| CN105950520A (en) * | 2016-07-18 | 2016-09-21 | 武汉市农业科学技术研究院作物科学研究所 | Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium |
| CN115474521A (en) * | 2022-09-22 | 2022-12-16 | 中国农业科学院草原研究所 | Alfalfa root system cultivation method based on nitrogen form |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
| CN101194567A (en) * | 2008-01-03 | 2008-06-11 | 中国农业大学 | Method for improving production volume and quality of alfalfa seed |
-
2012
- 2012-02-06 CN CN201210024784.2A patent/CN102523787B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
| CN101194567A (en) * | 2008-01-03 | 2008-06-11 | 中国农业大学 | Method for improving production volume and quality of alfalfa seed |
Non-Patent Citations (3)
| Title |
|---|
| 刘丽馥: "苜蓿根瘤菌在不同基质中与紫花苜蓿共生匹配的研究", 《东北农业大学硕士学位论文》 * |
| 刘彦江 等: "紫花苜蓿应用根瘤菌剂和稀土肥料效果试验", 《草业科学》 * |
| 李旭军 等: "紫花苜蓿专用根瘤菌剂使用技术研究", 《草业科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105144911A (en) * | 2015-09-28 | 2015-12-16 | 江苏农林职业技术学院 | Method for cultivating grass-like plant |
| CN105660108A (en) * | 2016-01-08 | 2016-06-15 | 山西省农业科学院高寒区作物研究所 | High-yield cultivation method for alfalfa in semi-arid region |
| CN105950520A (en) * | 2016-07-18 | 2016-09-21 | 武汉市农业科学技术研究院作物科学研究所 | Rhizobium capable of efficiently solubilizing phosphorus and application of rhizobium |
| CN105950520B (en) * | 2016-07-18 | 2019-05-10 | 武汉市农业科学技术研究院作物科学研究所 | A kind of rhizobium of efficient phosphate-solubilizing and its application |
| CN115474521A (en) * | 2022-09-22 | 2022-12-16 | 中国农业科学院草原研究所 | Alfalfa root system cultivation method based on nitrogen form |
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| CN102523787B (en) | 2014-04-16 |
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