CN113661885A - Method for relieving poisoning of lawn pennywort herb caused by cadmium stress - Google Patents
Method for relieving poisoning of lawn pennywort herb caused by cadmium stress Download PDFInfo
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- CN113661885A CN113661885A CN202110658620.4A CN202110658620A CN113661885A CN 113661885 A CN113661885 A CN 113661885A CN 202110658620 A CN202110658620 A CN 202110658620A CN 113661885 A CN113661885 A CN 113661885A
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- cadmium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
Abstract
The invention provides a method for relieving poisoning of hydrocotyle sibthorpioides by cadmium stress, which comprises the following steps of: selecting a lawn of lawn pennywort herb which has no scar and good growth vigor, cleaning soil at the root by using tap water, and then rinsing the soil by using deionized water for multiple times; selecting plants with approximate biomass and plant height for pre-culture; according to the pre-culture result, selecting plants with consistent growth vigor and transplanting the plants into a cadmium solution added with exogenous sulfur for culture; the plants are put under natural conditions of smooth ventilation and sufficient illumination for continuous aeration culture for 24 hours. By the method, the poisoning of cadmium stress on the lawn pennywort herb can be effectively relieved, and the biomass of the lawn pennywort herb is improved.
Description
Technical Field
The invention relates to the technical field of heavy metal stress growth of plants, in particular to a method for relieving the toxicity of cadmium stress on pennywort herbs in south America.
Background
In the development process of mineral resources, heavy metal pollution events occur frequently, which causes serious pollution to the environment, especially greater harm to farmlands and rivers. In heavy metal contaminated soil, the out-of-standard rate of cadmium (Cd) point sites is 7.0%, and the Cd point sites account for the first inorganic pollutants. Cadmium is generated along with other minerals and has the characteristics of easy enrichment, transportation and migration, so that the cadmium-containing material has great harm to human bodies, and the work of treating the cadmium-polluted environment is urgent.
In the current remediation of cadmium environmental pollution, there are mainly a physical method (a soil-moving method, a soil-changing method, a deep ploughing method, etc.), a chemical method (a solidification method, an application modifier method, an electrochemical method, etc.), and a biological method. The phytoremediation is a method for removing cadmium from the environment by absorbing, transporting and enriching heavy metal cadmium through plants with certain tolerance and enrichment characteristics, and belongs to one of biological methods. Compared with other repair measures, the plant repair has the advantages of safety, environmental protection, economy, sustainability and the like. With regard to the heavy metal absorption and enrichment capacity of plants, relatively few researches on the heavy metal absorption and enrichment aspect of garden plants are currently carried out. The lawn pennywort herb is a common landscaping plant, has the characteristics of high biomass, high growth speed and wide distribution range, can beautify the environment, has the function of purifying air and absorbs harmful gas; has application potential in the aspect of purifying sewage such as heavy metal sewage.
Disclosure of Invention
In order to solve the problem of cadmium pollution, the invention provides a method for relieving the poisoning of the hydrocotyle sibthorpioides by cadmium stress. The poisoning of cadmium stress on the pennywort herb is effectively relieved by adding exogenous sulfate, and the growth of the pennywort herb is promoted.
In order to achieve the purpose, the invention adopts the following technical scheme:
the method for relieving the poisoning of the hydrocotyle sibthorpioides caused by cadmium stress comprises the following steps:
(1) selecting a lawn of lawn pennywort herb which has no scar and good growth vigor, cleaning soil at the root by using tap water, and then rinsing the soil by using deionized water for multiple times;
(2) selecting plants with approximate biomass and plant height in the step (1) for pre-culture;
(3) transplanting the plants with consistent growth vigor in the step (2) into a cadmium solution added with exogenous sulfur;
(4) and (4) placing the plants in the step (3) under natural conditions of smooth ventilation and sufficient illumination for continuous aeration culture for 24 hours.
Further, when the southern American lawn pennywort herb is washed in the step (1), root soil and leaf surfaces are washed clean by using slow water flow of tap water to ensure that plants are not damaged, then deionized water is used for rinsing for 3 times, and residual water is wiped by using filter paper.
Further, when the step (2) is pre-cultured, a special total nutrient high-efficiency plant nutrient solution (Joule, Xuzhou Yaode chemical Co., Ltd.) for water culture is used as a medium when the step (2) is pre-cultured.
Further, the preculture time in step (2) was 7 days.
Furthermore, the dilution multiple of the special total-nutrient efficient plant nutrient solution (Torrel, Yaode chemical Co., Ltd., Xuzhou) for water culture is 400 times during the pre-culture in the step (2).
Further, the roots of the plants are immersed for 10min by using a disinfectant before the plants are transplanted in the step (3).
Further, the disinfectant is 0.1% KMnO4And (3) solution.
Further, in the step (3), Na is respectively added to the sulfur and the cadmium in the step2SO4(analytically pure) and CdCl2·2.5H2Added as O (analytically pure).
Further, three cadmium concentration gradients are set in the step (3), namely 1(Cd1), 2(Cd2) and 4(Cd4) mg.L-1Three sulfur concentrations, i.e., 0(S0), 200(S200), and 400(S400) μ M · L, are set for each cadmium concentration level-1。
In summary, the invention has the following advantages:
by adding exogenous sulfate, the poison of cadmium stress on the southern American lawn pennywort herb is effectively relieved, the net photosynthetic rate and the contents of chlorophyll a, chlorophyll b and carotenoid are improved, and the growth of the southern American lawn pennywort herb is promoted; wherein, under the Cd4 level, the net photosynthetic rate in S200 and S400 is improved by 30.54 percent and 42.86 percent, the chlorophyll a is improved by 15.38 percent and 40.77 percent, the chlorophyll b is improved by 9.52 percent and 33.3 percent, and the carotenoid is improved by 13.33 percent and 20 percent; the average cadmium concentration and the total cadmium content of the upper part of the plant water are reduced by 8.69 percent, 29.83 percent, 15.42 percent and 33.33 percent, and the average cadmium concentration and the total cadmium content of the lower part of the plant water are respectively reduced by 18.88 percent, 45.43 percent, 13.56 percent and 27.12 percent; the transport coefficient is improved by 11.43 percent and 28.57 percent.
Drawings
FIG. 1 shows the effect of sulfate on cadmium concentration and cadmium transport coefficient in Centipeda minima bodies under cadmium stress;
FIG. 2 is a graph of the effect of sulfate on the cadmium-enriched total amount of Centipeda minima under cadmium stress;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for relieving poisoning of Centipeda minima subjected to cadmium stress comprises the following steps:
(1) firstly, cleaning soil at the root and the leaf surface by using slow running water flow to ensure that plants are not damaged, then rinsing for 3 times by using deionized water, and wiping off residual water by using filter paper.
(2) Selecting plants with approximate biomass and plant height in the step (1) for pre-culture, wherein the culture time is 7 days, and using a special total-nutrient high-efficiency plant nutrient solution (Todele, Xuzhou Yaode chemical Co., Ltd.) with a dilution multiple of 400 times as a medium.
(3) Transplanting the plants with consistent growth vigor in the step (2) into a cadmium solution added with exogenous sulfur, and using 0.1 percent KMnO before transplanting4Immersing the root in disinfectant for 10min, and respectively soaking sulfur and cadmium in Mg2SO4(analytically pure) and CdCl2·2.5H2O (analytically pure) and three cadmium concentration gradients, namely 1(Cd1), 2(Cd2) and 4(Cd4) mg.L-1Adding 200 μ M.L per cadmium concentration level-1Na2SO4I.e., S200.
Example 2
A method for relieving poisoning of Centipeda minima subjected to cadmium stress comprises the following steps:
(1) firstly, cleaning soil at the root and the leaf surface by using slow running water flow to ensure that plants are not damaged, then rinsing for 3 times by using deionized water, and wiping off residual water by using filter paper.
(2) Selecting plants with approximate biomass and plant height in the step (1) for pre-culture, wherein the culture time is 7 days, and using a special total-nutrient high-efficiency plant nutrient solution (Todele, Xuzhou Yaode chemical Co., Ltd.) with a dilution multiple of 400 times as a medium.
(3) Transplanting the plants with consistent growth vigor in the step (2) into a cadmium solution added with exogenous sulfur, and using 0.1 percent KMnO before transplanting4Soaking the root with disinfectant solution for 10min, and respectively soaking the sulfur and cadmium with Na2SO4(analytically pure) and CdCl2·2.5H2O (analytically pure) and three cadmium concentration gradients, namely 1(Cd1), 2(Cd2) and 4(Cd4) mg.L-1Adding 400 μ M.L per cadmium concentration level-1Na2SO4I.e., S400.
Comparative example
A method of mitigating cadmium stress poisoning of pennywort, different from example 1, comprising the steps of:
(1) firstly, cleaning soil at the root and the leaf surface by using slow running water flow to ensure that plants are not damaged, then rinsing for 3 times by using deionized water, and wiping off residual water by using filter paper.
(2) Selecting plants with approximate biomass and plant height in the step (1) for pre-culture, wherein the culture time is 7 days, and using a special total-nutrient high-efficiency plant nutrient solution (Todele, Xuzhou Yaode chemical Co., Ltd.) with a dilution multiple of 400 times as a medium.
(3) Transplanting the plants with consistent growth vigor in the step (2) into a cadmium solution added with exogenous sulfur, and using O.1% KMnO before transplanting4Soaking the root with disinfectant solution for 10min, and respectively soaking the sulfur and cadmium with Na2SO4(analytically pure) and CdCl2·2.5H2Addition in the form of O (analytically pure), threeThe cadmium concentration gradient is 1(Cd1), 2(Cd2) and 4(Cd4) mg.L-1No Na is added at each cadmium concentration level2SO4I.e. SO.
The results of the indicators of the growth and photosynthetic properties of pennywort herbs in example 1, example 2 and comparative example are as follows:
note: different lower case english letters in the same column indicate that the same cadmium and different sulfate supply levels have significant differences (P < 0.05); different capital english letters in the same column indicate different cadmium levels, with significant differences in the same sulfate supply (P < 0.05).
Claims (9)
1. A method for relieving poisoning of Centipeda minima subjected to cadmium stress is characterized by comprising the following steps:
(1) selecting a lawn of lawn pennywort herb which has no scar and good growth vigor, cleaning soil at the root by using tap water, and then rinsing the soil by using deionized water for multiple times;
(2) selecting plants with approximate biomass and plant height in the step (1) for pre-culture;
(3) transplanting the plants with consistent growth vigor in the step (2) into a cadmium solution added with exogenous sulfur;
(4) and (4) placing the plants in the step (3) under natural conditions of smooth ventilation and sufficient illumination for continuous aeration culture for 24 hours.
2. The method for relieving the poisoning effect of cadmium stress on the southern American lawn pennywort herb as claimed in claim 1, wherein when the southern American lawn pennywort herb is washed in the step (1), the soil and the leaf surface of the root are washed clean by using a slow running water flow to ensure that the plant is not damaged, then the southern American lawn pennywort herb is rinsed for 3 times by using deionized water, and residual water is sucked by using filter paper.
3. The method for alleviating the poisoning effect of cadmium stress on southern American lawn pennywort herb as claimed in claim 1, wherein the pre-culture in the step (2) uses a special total nutrient high-efficiency plant nutrient solution (Ledele, Yaode chemical Co., Xuzhou) for water culture as a medium.
4. The method for alleviating poisoning of Centipeda minima by cadmium stress according to claim 1, wherein the preculture time in step (2) is 7 days.
5. The method for alleviating the poisoning effect of cadmium stress on the southern American lawn pennywort herbs as claimed in claim 3, wherein the dilution factor of the special total nutrient high-efficiency plant nutrient solution for water culture (Ledele, Yaode chemical Co., Ltd., Xuzhou) is 400 times.
6. The method for alleviating poisoning of hydrocotyle hybridus under cadmium stress according to claim 1, wherein the roots of the plants are immersed in a disinfectant for 10min before being transplanted in the step (3).
7. The method of claim 6, wherein the disinfection solution is 0.1% KMnO4And (3) solution.
8. The method for alleviating poisoning of Centipeda minima by cadmium stress according to claim 1 wherein in step (3) the sulphur and cadmium are Na and Na respectively2SO4(analytically pure) and CdCl2·2.5H2Added as O (analytically pure).
9. The method for alleviating poisoning of Centipeda minima under cadmium stress according to claim 1, wherein three cadmium concentration gradients are set in step (3), namely 1(Cd1), 2(Cd2), 4(Cd4) mg.L-1Three sulfur concentrations, i.e., 0(S0), 200(S200), and 400(S400) μ M · L, are set for each cadmium concentration level-1。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114467727A (en) * | 2022-01-19 | 2022-05-13 | 陕西科技大学 | Application of sulfate ions in relieving cadmium stress of tomato seedlings |
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Application publication date: 20211119 |