CN102601102A - Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium - Google Patents
Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium Download PDFInfo
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- CN102601102A CN102601102A CN2012100281415A CN201210028141A CN102601102A CN 102601102 A CN102601102 A CN 102601102A CN 2012100281415 A CN2012100281415 A CN 2012100281415A CN 201210028141 A CN201210028141 A CN 201210028141A CN 102601102 A CN102601102 A CN 102601102A
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Abstract
The invention discloses a method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium, concretely comprising the the steps of: inoculating an arbuscular mycorrhizal fungi inoculant such as Glomus mosseae bacteria or Glomus versiforme bacteria and a mixed inoculant of petroleum hydrocarbon and degradation bacterium liquid in the high-concentration oil-polluted soil and planting mycorrhizal fungi aulophytes such as shamrocks and corns, growing for 90-125 days, and harvesting corn plant bodies with high biomass liveweight and burning in a centralized manner. The method has the advantage of establishing a composite mycorrhizal fungi-plant-degradation bacterium co-metabolism system; on one side, the mycorrhizal fungi and plants form mutually beneficial symbionts so as to help the plants to absorb nutrition and water and promote the growth of the plants so that the stress resistance of the plants to the high-concentration oil-polluted soil; on the other side, the mycorrhizal fungi is used as heterotrophic microorganism and the petroleum hydrocarbon is used as exogenous carbon which is decomposed and utilized; and moreover, nutrients are provided to exogenous petroleum hydrocarbon degradation bacteria through mycorrhizal fungi and the secretions of plant root cells so that the degradation efficiency of the petroleum hydrocarbon is improved.
Description
Technical field
The present invention relates to contaminated soil recovery technique field, be specifically related to the method that a kind of mycorrhizal fungi-plant-degradation bacteria is repaired high concentration petroleum pollution soil.
Background technology
China is the big country of Petroleum Production and consumption in the world, yet the thing followed is serious day by day soil oil pollution problem.Owing to reasons such as the storage of oil and transportation, petroleum refining, leakage accident, oil-polluted water irrigations, oil-polluted soils increases year by year; It is reported that China has 6 * 10 every year approximately
5The t oil gets into environment through approach such as run, drip, leak, causes soil pollution; In addition, the contaminated area of every mouthful of oil-producing well reaches 500m
2, and oil-producing area crude oil pollution area is also enlarging the heavily contaminated district in some oil fields, soil crude content height to 1 * 10 year by year
4Mg/kg, the critical limit 200mg/kg that can bear of soil head and shoulders above, the soil ecosystem of IA is seriously damaged, so the reparation problem of high concentration petroleum pollution soil is extremely urgent.
At present, the restorative procedure of oil-polluted soils can roughly be divided into physics method, chemical method and bioanalysis etc.And the bioremediation technology application prospect is the most optimistic; But in-situ treatment; And cost is low, non-secondary pollution, and biological renovation methods such as single microorganism, plant or plant-microorganism are all because of the biological environment that is difficult to stand high pollution; And microbial reproduction or the poky phenomenon of plant occur, and then cause the low problem of degradation efficiency in the short time; So, biological energy source is stood pollutant and can in contaminated environment, be survived fast and just become the basis of biological restoration contaminated environment with growth.The growth of plant is determined with the nutrients quality that is absorbed by the soil quantity of root system contact to a great extent; AMF has stronger patience to the pollutant in the soil, can increase root exudates and improve soil texture, and have the characteristics of degraded and transfer environmental contaminants; Can form mycorrhizas homobium with plant; And the mycelia of its extension can increase the contact-making surface of root system of plant and soil, promotes the absorption of plant to nutrition, in addition; AMF is as heterotrophic microorganism, can organic pollution is decomposed into simple organic matter and carbon hydrate etc. as external source carbon and be used; The secretion that juice constituted of resulting nutrients of AMF decomposing pollutant and plant roots cell is that exogenous petroleum hydrocarbon degradation bacterium provides the nutrition and the energy, has promoted the breeding of petroleum hydrocarbon degradation bacterium and then has improved its degradation efficiency to petroleum hydrocarbon.
Summary of the invention
The present invention is directed to high concentration petroleum pollution soil, the method for a kind of mycorrhizal fungi-plant-compound reparation of degradation bacteria is provided.
The object of the invention realizes through following technical scheme.
A kind of mycorrhizal fungi-plant-degradation bacteria is repaired the method for high concentration petroleum pollution soil, it is characterized in that, may further comprise the steps:
(1) inoculation AMF microbial inoculum in oil-polluted soils;
(2) sowing clover and corn in oil-polluted soils;
(3) at the rhizosphere place of clover and corn inoculation petroleum hydrocarbon degradation bacteria agent;
(4) waited to grow 90～125 days after, the plant of harvesting corn concentrate to burn.
Said AMF microbial inoculum is for containing the sandy soil microbial inoculum of Glomus mosseae bacterium (Glomus mosseae) or face of land sacculus mould (Glomus versifome) spore and mycelia, and inoculative proportion is that every 2.0kg oil-polluted soils adds AMF microbial inoculum 60g.
Said petroleum hydrocarbon degradation bacteria agent is pseudomonas putida (Pseudomonas putida), pseudomonas pseudoalcaligenes (Pseudomonas pseudoalkaligenes), pseudomonas aeruginosa (Pseudomonas aeruginosa) and whiterot fungi (Phanerochate chrysosporium) 1: 1: 1 by volume: the 1 mixing material microbial inoculum formed.
Said oil-polluted soils PetroChina Company Limited. hydrocarbon content is 1 * 10
3～1 * 10
4Mg/kg.
The inoculative proportion of said petroleum hydrocarbon degradation bacteria agent is that every 2.0kg oil-polluted soils adds petroleum hydrocarbon degradation bacteria agent 200ml;
The present invention is with respect to advantage and effective effect that prior art had:
The present invention has set up the compound metabolic system altogether of mycorrhizal fungi-plant-degradation bacteria; In this system, AMF and plant form the homobium of mutual reciprocity and mutual benefit, the external mycelia network that mycorrhizal fungi forms; Can increase root and the native amount of contact; Help plant from soil, to absorb mineral nutrition and moisture, promote the plant growth, improve the resistance of plant the high concentration petroleum hydrocarbon; AMF is as heterotrophic microorganism, can organic pollution is decomposed into simple organic matter and carbon hydrate etc. as external source carbon and be used; In addition, the secretion that juice constituted of resulting nutrients of AMF decomposing pollutant and plant roots cell provides the nutrition and the energy for the external source petroleum hydrocarbon degradation bacterium, and then improves its degradation efficiency to petroleum hydrocarbon.
The present invention is total to the soil that metabolic system is applicable to high concentration petroleum pollution in addition, and petroleum hydrocarbon content is 1 * 10
3～1 * 10
4Between the mg/kg; Solved in the high concentration petroleum pollution soil, plant fixing difficulty, poor growth and the low problems such as (10-20%) of remediation efficiency, the remediation efficiency of native system in a reparation phase can reach 45%～65%.
The specific embodiment
Used AMF microbial inoculum in the instance is to utilize in the greenhouse, to expand numerous gained available from " Chinese AMF germplasm resource bank (BGC) " bacterial classification and clover, dry river sand.
Used petroleum hydrocarbon degradation bacteria agent is the mixing material microbial inoculum that pseudomonas putida (Pseudomonas putida), pseudomonas pseudoalcaligenes (Pseudomonas pseudoalkaligenes), pseudomonas aeruginosa (Pseudomonas aeruginosa) and whiterot fungi (Phanerochate chrysosporium) were formed in 1: 1 by volume in the instance, and single pure culture is all purchased in Chinese common micro-organisms bacterial classification and preserved administrative center.
Instance 1: the oil-polluted soils 2.0kg that takes by weighing petroleum hydrocarbon content and be 1998.6mg/kg is loaded in the flowerpot, sneaks into Glomus mosseae bacterium (Glomus mosseae) microbial inoculum 60g, regulates soil moisture content 50%, hold over night.Clover and corn seed are through 10%H
2O
2After surface sterilization and the vernalization, be sowed in the soil of inoculating the group planting mycorrhizal fungi, application rate is 15 clover and 5 corn/basins; Treat that it carries out thinning when sprouting to tri-leaf period; Every basin stays 8 strain clover, 2 strain corns, the inoculation petroleum hydrocarbon degradation bacteria agent at the plant rhizosphere place simultaneously; The every basin 200ml of microbial inoculum consumption then adopts the way to manage of common crops; Collection soil and corn plant sample are analyzed after 124 days, estimate repairing effect.
Result of study shows, through 124 days cultivation, compares with control treatment, and the petroleum hydrocarbon degradation rate under the hybrid system in the soil obviously improves, and can reach 62.1%.
Instance 2: the oil-polluted soils 2.0kg that takes by weighing petroleum hydrocarbon content and be 1998.6mg/kg is loaded in the flowerpot, sneaks into face of land sacculus mould (Glomus versifome) microbial inoculum 60g, regulates soil moisture content 50%, hold over night.Clover and corn seed are through 10%H
2O
2After surface sterilization and the vernalization, be sowed in the soil of inoculating the group planting mycorrhizal fungi, application rate is 15 clover and 5 corn/basins; Treat that it carries out thinning when sprouting to tri-leaf period; Every basin stays 8 strain clover, 2 strain corns, the inoculation petroleum hydrocarbon degradation bacteria agent at the plant rhizosphere place simultaneously; The every basin 200ml of microbial inoculum consumption then adopts the way to manage of common crops; Collection soil and corn plant sample are analyzed after 124 days, estimate repairing effect.
Result of study shows, through 124 days cultivation, compares with control treatment, and the petroleum hydrocarbon degradation rate under the hybrid system in the soil obviously improves, and can reach 63.05%.
Instance 3: each 2.0kg of oil-polluted soils that to take by weighing two parts of petroleum hydrocarbon content be 9860mg/kg is loaded on respectively in two flowerpots; Sneak into Glomus mosseae bacterium (Glomus mosseae) and face of land sacculus mould (Glomus versifome) microbial inoculum 60g respectively; Regulate soil moisture content all 50%, hold over night.Clover and corn seed are through 10%H
2O
2After surface sterilization and the vernalization, be sowed in the soil of inoculating the group planting mycorrhizal fungi, the application rate of every basin is 15 clover and 5 corns; Treat that it carries out thinning when sprouting to tri-leaf period; Every basin stays 8 strain clover, 2 strain corns, the inoculation petroleum hydrocarbon degradation bacteria agent at the plant rhizosphere place simultaneously; The every basin 200ml of microbial inoculum consumption then adopts the way to manage of common crops; Collection soil and corn plant sample are analyzed after 124 days, estimate repairing effect.
Result of study shows; Cultivation through 124 days; Compare with control treatment; Petroleum hydrocarbon degradation rate under the hybrid system in the soil obviously improves, and the petroleum hydrocarbon degradation rate that inoculation Glomus mosseae bacterium (Glomus mosseae) is handled is 47.61%, and the degradation rate that inoculation face of land sacculus mould (Glomus versifome) is handled reaches 51.20%.
Claims (5)
1. the method that mycorrhizal fungi-plant-degradation bacteria is repaired high concentration petroleum pollution soil is characterized in that, may further comprise the steps:
(1) inoculation AMF microbial inoculum in oil-polluted soils;
(2) sowing clover and corn in oil-polluted soils;
(3) at the rhizosphere place of clover and corn inoculation petroleum hydrocarbon degradation bacteria agent;
(4) waited to grow 90～125 days after, the plant of harvesting corn concentrate to burn.
2. method according to claim 1; It is characterized in that; Said AMF microbial inoculum is for containing the sandy soil microbial inoculum of Glomus mosseae bacterium (Glomus mosseae) or face of land sacculus mould (Glomus versifome) spore and mycelia, and inoculative proportion is that every 2.0kg oil-polluted soils adds AMF microbial inoculum 60g.
3. method according to claim 1; It is characterized in that said petroleum hydrocarbon degradation bacteria agent is pseudomonas putida (Pseudomonas putida), pseudomonas pseudoalcaligenes (Pseudomonas pseudoalkaligenes), pseudomonas aeruginosa (Pseudomonas aeruginosa) and whiterot fungi (Phanerochate chrysosporium) 1: 1: 1 by volume: the 1 mixing material microbial inoculum formed.
4. method according to claim 1 is characterized in that, said oil-polluted soils PetroChina Company Limited. hydrocarbon content is 1 * 10
3～1 * 10
4Mg/kg.
5. method according to claim 1 is characterized in that, the inoculative proportion of said petroleum hydrocarbon degradation bacteria agent is that every 2.0kg oil-polluted soils adds petroleum hydrocarbon degradation bacteria agent 200ml.
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Cited By (10)
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|---|---|---|---|---|
| CN103042025A (en) * | 2013-01-04 | 2013-04-17 | 青岛科技大学 | Method for restoring polluted soil through transgenic plant-gliocladium roseum system |
| CN103146608A (en) * | 2013-03-06 | 2013-06-12 | 青岛农业大学 | AMF+PGPR (arbuscular mycorrhizal fungi+plant growth promoting rhizobacteria) combined microbial inoculum and preparation method and application of combined microbial inoculum in decomposition of residual organophosphorus pesticides decomposition |
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| CN104525538A (en) * | 2014-12-11 | 2015-04-22 | 同济大学 | Method for dryly cleaning petroleum polluted cloth bag by using alkaline silt |
| CN105290105A (en) * | 2015-12-10 | 2016-02-03 | 郭嘉川 | Method for degrading grease dirt in wetland |
| CN105665438A (en) * | 2016-01-13 | 2016-06-15 | 三峡大学 | Method for repairing soil severely polluted by petroleum hydrocarbon through plants |
| CN105750312A (en) * | 2016-04-26 | 2016-07-13 | 天津大学 | Application optimization method of local soil plant-microorganism remediation technology |
| CN112207126A (en) * | 2020-09-04 | 2021-01-12 | 福建滨海生物科技有限公司 | Integrated process for ecological restoration of soil environment |
| CN113714274A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Plunger pump for microorganism soil layer dispersion mixing inoculation |
| CN113714275A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer multistage inoculation microorganism regulation and control method |
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| CN103146608A (en) * | 2013-03-06 | 2013-06-12 | 青岛农业大学 | AMF+PGPR (arbuscular mycorrhizal fungi+plant growth promoting rhizobacteria) combined microbial inoculum and preparation method and application of combined microbial inoculum in decomposition of residual organophosphorus pesticides decomposition |
| CN103146608B (en) * | 2013-03-06 | 2014-09-10 | 青岛农业大学 | AMF+PGPR (arbuscular mycorrhizal fungi+plant growth promoting rhizobacteria) combined microbial inoculum and preparation method and application of combined microbial inoculum in decomposition of residual organophosphorus pesticides decomposition |
| CN103480644A (en) * | 2013-10-12 | 2014-01-01 | 南开大学 | Plant-microorganism combined method for enhanced repairing of petroleum-contaminated spetroleum |
| CN104525538A (en) * | 2014-12-11 | 2015-04-22 | 同济大学 | Method for dryly cleaning petroleum polluted cloth bag by using alkaline silt |
| CN105290105A (en) * | 2015-12-10 | 2016-02-03 | 郭嘉川 | Method for degrading grease dirt in wetland |
| CN105290105B (en) * | 2015-12-10 | 2018-10-19 | 上海秦森园林股份有限公司 | A kind of method of greasy dirt in degradation wetland |
| CN105665438A (en) * | 2016-01-13 | 2016-06-15 | 三峡大学 | Method for repairing soil severely polluted by petroleum hydrocarbon through plants |
| CN105665438B (en) * | 2016-01-13 | 2018-06-19 | 三峡大学 | A kind of method of the soil petroleum hydrocarbon contaminated using phytoremediation severe |
| CN105750312A (en) * | 2016-04-26 | 2016-07-13 | 天津大学 | Application optimization method of local soil plant-microorganism remediation technology |
| CN113714274A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Plunger pump for microorganism soil layer dispersion mixing inoculation |
| CN113714275A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer multistage inoculation microorganism regulation and control method |
| CN112207126A (en) * | 2020-09-04 | 2021-01-12 | 福建滨海生物科技有限公司 | Integrated process for ecological restoration of soil environment |
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Application publication date: 20120725 |