CN105750312A - Application optimization method of local soil plant-microorganism remediation technology - Google Patents
Application optimization method of local soil plant-microorganism remediation technology Download PDFInfo
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- CN105750312A CN105750312A CN201610268201.9A CN201610268201A CN105750312A CN 105750312 A CN105750312 A CN 105750312A CN 201610268201 A CN201610268201 A CN 201610268201A CN 105750312 A CN105750312 A CN 105750312A
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- soil
- petroleum hydrocarbon
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
The invention discloses an application optimization method of a local soil plant-microorganism remediation technology.The method comprises the following steps that step (1), local natural rainfall, soil depth and moisture content parameters for soil remediation are determined; step (2), a soil remediation model is built; step (3), the application effects of the remediation technology under different conditions are simulated according to the local characteristics and by the combination of the soil remediation model; step (4), the soil remediation model suitable for local conditions is selected, and suitable remediation measures are adopted; step (5), optimized parameters of initial pollution concentration and irrigation and plowing depth are reasonably determined, the plant and microorganism degradation capabilities are determined, and the optimized remediation technology scheme is applied.According to the method, temporal and spatial variations of to-be-remediated pollutants, plants and bacterial strains in contaminated soil are determined, key factors affecting the remediated pollutant degradating effect are found out, the optimized remediation technology scheme matched with the local conditions is put up, and the remediation efficiency and the remediation effect are improved.
Description
Technical field
The present invention relates to contaminated soil remediation, recovery technique security fields technical field, particularly relate to a kind of local soil
The optimizing application method of plant-microorganism recovery technique.
Background technology
The research utilizing plant-microorganism combine d bioremediation contaminated soil is the most a lot.Such as, during petroleum hydrocarbon residues in soil
The most degradable, remove difficulty big.Utilize plant-microorganism to repair petroleum hydrocarbon contaminated soil and there is the advantages such as non-secondary pollution,
But the most time-consuming permanent, degradation effect is worse than other physico-chemical process.Therefore, according to varying environment and recovery technique because of
Element, determines pollutant to be repaired and plant, the change in time and space of bacterial strain in contaminated soil, finds out impact and repairs contaminant degradation
The key factor of effect, proposes the optimization recovery technique scheme mated with local condition, has important to improving remediation efficiency
Using value.
Bioavailability and coupling local condition degree are to affect restoration of the ecosystem, improve the key of contaminant degradation.This
Patent, as a example by the plant-microorganism combined remediation technology optimizing petroleum hydrocarbon contaminated soil, is given and how to find out control pollution
The key factor of thing degradation effect, proposes the system of selection of the optimization recovery technique scheme mated with local condition.
Summary of the invention
Based on above-mentioned prior art and the problem of existence, the present invention proposes the plant-microorganism reparation of a kind of local soil
The optimizing application method of technology, is determined by soil remediation model, finds out petroleum hydrocarbon contaminant impact, proposes to reach pollution
The reclamation activities of the key factor of thing degradation effect.
The present invention proposes the optimizing application method of the plant-microorganism recovery technique of a kind of local soil, and the method includes
Following steps:
Step 1, determine that meteorology (natural precipitation amount) that soil remediation works as and soil important parameter are (depth of soil, aqueous
Rate)；
Step 2, set up soil remediation model
Soil remediation model one, the selection soil lower boundary degree of depth, if petroleum hydrocarbon concentration has exceeded the pollution background value preset,
Representing in the case of high concentration is petroleum hydrocarbon contaminated, lower boundary degree of depth soil below is contaminated, deep in conjunction with further checking
The layer petroleum hydrocarbon contaminated degree of soil and scope, use soil to blend the reclamation activities reducing pollutant levels；
Soil remediation model two, when the half that petroleum hydrocarbon peak concentration reduces to initial concentration being detected, show existing
Under repairing condition, low concentration is the most petroleum hydrocarbon contaminated reaches repairing effect；
Soil remediation model three, binding tests district condition of raining, for reaching petroleum hydrocarbon degradation rate, use adjust irrigation volume and
The reclamation activities of design irrigation method；
Soil remediation model four, binding tests district locality feature, for reaching the microbial degradation amount optimized, use and adjust soil
The reclamation activities of earth ploughed depth；
Step 3, according to local feature, in conjunction with above-mentioned soil remediation model, under simulation different condition, recovery technique should
Use effect；
Step 4, selection adapt to the soil remediation model of local condition, use the reclamation activities being suitable for；
Step 5, rationally determine the Optimal Parameters of initial contamination concentration, irrigation and ploughed depth, determine plant and microorganism
Degradation capability, implements the recovery technique scheme optimized.
Compared with prior art, the advantage of technical solution of the present invention is: be determined by pollutant to be repaired in contaminated soil
With plant, the change in time and space of bacterial strain, find out impact and repair the key factor of contaminant degradation effect, propose and local condition
The optimization recovery technique scheme of coupling, improves remediation efficiency and repairing effect.
Accompanying drawing explanation
Fig. 1 is high concentration situation soil Petroleum Hydrocarbon concentration distribution schematic diagram；
Marginal data: (---) represents the 0th day；(--) represent the 30th day；() represents the 60th
My god；() represents the 90th day；(...) represents the 120th day；(------) represents the 150th day；(——)
Represent the 180th day；
Fig. 2 is low concentration pollution situation soil Petroleum Hydrocarbon concentration distribution schematic diagram；
Marginal data: (---) represents the 0th day；(--) represent the 30th day；() represents the 60th
My god；() represents the 90th day；(...) represents the 120th day；(---) represents the 150th day；()
Represent 180 days；
Fig. 3 is the 180th day petroleum hydrocarbon concentration profiles versus's schematic diagram in the case of irrigating and not irrigating；
Marginal data: (---) represents 10 times/10mm irrigation every time；(------) represents 10 times/each 20mm
Irrigate；() expression is not irrigated)；
Fig. 4 is the 180th day petroleum hydrocarbon concentration profiles versus's schematic diagram under different designs sight；
Marginal data: (--) represents sight 1；() represents sight 2；(---) represents sight 3；(——)
Represent natural precipitation.
Fig. 5 is the 180th day petroleum hydrocarbon concentration profiles versus's schematic diagram under different ploughed depth；(--) represents 5cm；
() represents 10cm；(---) represents 15cm；() represents 20cm；
Fig. 6 is the overall flow of the optimizing application method of the plant-microorganism recovery technique of a kind of local soil of the present invention
Schematic diagram.
Detailed description of the invention
Below in conjunction with the drawings and the specific embodiments, it is described in further detail technical scheme.
The microorganism of the present invention includes Brevibacillus panacihumi Strain W25 and Gordonia alkanivorans
StrainW33, plant is that alkali is fluffy.
The process using Hydrus software that Suaeda salsa-microbial association is repaired petroleum hydrocarbon contaminated soil is optimized mould
Intend, i.e. simulation petroleum hydrocarbon contaminant principal alkane component C16、Pr、Ph、C22、C24、C26、C28And C30Space-time
Migrate and degradation process, then carry out data process by excel.
1, petroleum hydrocarbon initial contamination concentration impact
Set petroleum hydrocarbon contaminated initial concentration as low concentration 500mg/kg and high concentration 10000mg/kg, and with middle concentration
The degradation effect of 2000mg/kg contrasts, it is assumed that the Soil tillage degree of depth is 20cm.It is dirty that Fig. 1 gives high concentration
In the case of dye, petroleum hydrocarbon concentration is with the change of the Soil tillage degree of depth.Select 40cm depth as lower boundary, petroleum hydrocarbon concentration
Reached 1120.03mg/kg, exceeded pollution background value, it is seen that in the case of high concentration is petroleum hydrocarbon contaminated, 40cm with
The lower contaminated impact of soil can not be ignored, and underground water may be produced and pollute by petroleum hydrocarbon, now needs to verify deep layer further
The petroleum hydrocarbon contaminated degree of soil and scope, determine reclamation activities.
Fig. 2 gives in the case of low concentration pollution petroleum hydrocarbon concentration with the change of depth of soil.Visible at the 180th day, stone
Petroleum hydrocarbon peak concentration has reduced to the half of initial concentration, and under existing repairing condition, low concentration is the most petroleum hydrocarbon contaminated
Preferable effect can be reached.This also points out, and when repairing high concentration soil pollution, soil can be used to blend and reduce pollution
The way reparation of substrate concentration.
From low concentration to high density pollution, petroleum hydrocarbon degradation amount increases, but degradation rate reduces, and removal rate of petroleum hydrocarbons is respectively
50.74%, 37.94% and 29.32%.And microbial degradation rate is respectively 19.50%, 22.62% and 24.41%, in increasing
Main trend, is available for, this is because add, the petroleum hydrocarbon amount that microorganism utilizes.The fluffy clearance to petroleum hydrocarbon of alkali is respectively
30.73%, 14.82% and 4.14%, taper off trend, illustrates that petroleum hydrocarbon concentration exceedes alkali fluffy to its Exploitative potential.
In the case of low concentration, middle concentration to high concentration, petroleum hydrocarbon peak concentration respectively appear in 26.4cm, 24.8cm with
And at 23.2cm depth of soil, illustrate along with the increase of petroleum hydrocarbon concentration, its bulk migration reduced rate.No matter oil
Hydrocarbon concentration level, under earth's surface, about 25cm soil petroleum hydrocarbon residual quantity is in peak value all the time, can near herein suitably
Increase the quantity of microorganism, improve microbial degradation ability.
2, the impact of irrigation method
Design Different Irrigation processes, and increases soil moisture content, to obtain petroleum hydrocarbon home to return under different soils water condition.
Designing two kinds of irrigation methods is: irrigate 10 times, each 10mm, irrigates 10 times, each 20mm, and with do not irrigate
Degradation effect contrast.Petroleum hydrocarbon initial concentration is 2000mg/kg.
Never irrigate, 10mm irrigates and 20mm irrigates, and removal rate of petroleum hydrocarbons is respectively 37.94%, 37.96% He
37.13%.Microbial degradation rate is respectively 22.62%, 23.11% and 23.72%, in increase tendency, illustrates that irrigation makes stone
The bioavailability of petroleum hydrocarbon adds, and the fluffy clearance to petroleum hydrocarbon of alkali is respectively 14.82%, 14.00% He
10.27%, taper off trend, and this is owing to irrigation promotes to be seeped into the undeveloped district of root system or nothing under petroleum hydrocarbon is with moisture leaching
Root system district.Visible, suitably irrigate and can promote petroleum hydrocarbon degradation, but owing to root system of plant is shallower, with the stone oozed under moisture
Petroleum hydrocarbon can not get plant and utilizes.Fig. 3 is the 180th day petroleum hydrocarbon concentration profiles versus under different situations, increases and irrigates, stone
Petroleum hydrocarbon peak concentration substantially migrates downward into.Visible, although suitably to irrigate and can improve petroleum hydrocarbon degradation rate, but increase simultaneously
Its amount of migrating downward into.Inappropriate irrigation will cause petroleum hydrocarbon contaminated deep soil, even jeopardize underground water.Drop in trial zone
Under the conditions of rain, increase 10mm irrigation volume in dry season the most suitable.
Due to natural precipitation concentrate on 7, August, the irrigation method optimized for research, design drought-hit area Irrigation is (i.e.
Do not consider rainfall).Poor in view of clay pervasion, too much irrigation can reduce the gas permeability of soil, is unfavorable for petroleum hydrocarbon
Biodegradable, therefore, the soil moisture content to be controlled when Irrigation Design.
Ignore natural precipitation, irrigated once every 3 days, each irrigation volume 10mm.Irrigate at this under sight, petroleum hydrocarbon
Peak concentration migrates downward into relatively slow, and below 30cm soil is little affected by polluting.By the 180th day, Soil Microorganism dropped
Solving and alkali fluffy removal petroleum hydrocarbon accounts for the 22.09% of total amount, 26.27% respectively, petroleum hydrocarbon total removal rate is 48.36%, removes
Effect significantly improves.Visible, it is feasible that this irrigates Situated design.
Ignore natural precipitation, irrigated once every 3 days, each irrigation volume 15mm.Irrigate at this under sight, petroleum hydrocarbon
Peak concentration migrates downward into quickening, and at 40cm, the existing pollutant of soil occurs, pollution range increases.By the 180th day,
Soil Microorganism degraded and alkali fluffy removal petroleum hydrocarbon account for the 24.32% of total amount, 12.89% respectively, petroleum hydrocarbon total removal rate
Being 37.28%, removal effect is almost unchanged.Reason has two, and one is that total irrigation volume is too much, is gradually gone out by pollutant
Root system scope, reduces root system effect, and two is to ignore root growth, it is contemplated that test early stage root system is undeveloped, Ying Shi
When reducing irrigation volume.This irrigates Situated design needs to be adjusted.
On the basis of both the above Irrigation Design, it is considered to ensure total petroleum hydrocarbon clearance and reduce factors such as polluting peak value, if
It is as follows that sight irrigated by meter: ignores natural precipitation, irrigated once every 3 days, first 30 days each irrigation volume 10mm, with
Rear irrigation volume 15mm every time.Irrigating under sight at this, petroleum hydrocarbon peak concentration migrates downward into speed between both the above feelings
Between scape, at 40cm, the existing pollutant of soil occurs, but concentration is the lowest.To microbial degradation in the 180th day with alkali is fluffy goes
Except the petroleum hydrocarbon amount of accounting for respectively 23.67%, 17.54%.Soil Petroleum Hydrocarbon total removal rate is 41.22%, and petroleum hydrocarbon is removed
Effect is preferable.
Fig. 4 designs down the 180th day petroleum hydrocarbon concentration profiles versus for Different Irrigation, as seen from the figure, by Irrigation Design,
Overall repairing effect is all an advantage over natural precipitation situation.Under sight 3 Irrigation Design, soil Petroleum Hydrocarbon peak concentration
Less than sight 1, pollution range and migration velocity again less than sight 2, its repairing effect is preferable.By Rational Irrigation control
Soil moisture content, it is possible to reduce soil moisture and pollutant runs off, and improve repairing effect.
The impact of 3, soil pollution ploughed depth
Petroleum hydrocarbon contaminated concentration is very big on degraded impact, and root system of plant on the impact of degradation effect it is also obvious that turn over deep
Degree affects petroleum hydrocarbon contaminated concentration and root system effect simultaneously, accordingly, it is determined that suitably ploughed depth is critically important.If petroleum hydrocarbon
The initial contamination degree of depth is 5cm, and concentration is 2000mg/kg, separately designs the Soil tillage degree of depth and is: 5cm, 10cm, 15cm
And 20cm.The Soil tillage after stain degree of depth becomes at the beginning of 5.8cm, 11.6cm, 17.4cm and 23.2cm, petroleum hydrocarbon respectively
Beginning pollution concentration is respectively 2000mg/kg, 1000mg/kg, 666.67mg/kg and 500mg/kg.
Turn over 5cm by contaminated soil, and when the 120th day, soil Petroleum Hydrocarbon concentration can reach soil originally the most substantially
Floors 500mg/kg.Below soil 30cm is not the most by petroleum hydrocarbon contaminated.By the 180th day, Soil Microorganism dropped
Solve and alkali fluffy removal petroleum hydrocarbon accounts for the 18.09% of total amount, 48.44% respectively.It is visible, owing to the fluffy root system of alkali is at underground 5cm
The most flourishing, and the root system degree of depth is much larger than ploughed depth, the fluffy accumulative removal amount to petroleum hydrocarbon of alkali is greatly increased.Petroleum hydrocarbon
Total removal rate is 66.53%, and removal effect is good.
Turn over 10cm by contaminated soil, and when the 120th day, soil Petroleum Hydrocarbon concentration entirely reached background values 500
mg/kg.Below soil 30cm is substantially free of contaminants.By the 180th day, Soil Microorganism degraded and alkali fluffy removal stone
Petroleum hydrocarbon accounts for the 18.42% of total amount, 38.56% respectively.Visible, due to the increase of ploughed depth, alkali is fluffy to be tired out petroleum hydrocarbon
Long-pending removal amount slightly reduces.Petroleum hydrocarbon total removal rate is 56.98%.
Turn over 15cm by contaminated soil, and when the 90th day, soil Petroleum Hydrocarbon concentration entirely reached background values 500
mg/kg.Below soil 40cm starts by light contamination.By the 180th day, Soil Microorganism degraded was gone with alkali is fluffy
The 19.66% of total amount, 31.38% is accounted for respectively except petroleum hydrocarbon.Petroleum hydrocarbon total removal rate is 51.20%.
Turn over 20cm by contaminated soil, makes soil Petroleum Hydrocarbon concentration reach background values 500mg/kg although turning over, but
Below soil 40cm is increased the weight of by petroleum hydrocarbon contaminated.By the 180th day, Soil Microorganism degraded and alkali fluffy removal oil
Hydrocarbon accounts for the 20.20% of total amount, 26.62% respectively.Visible, along with the increase of ploughed depth, the fluffy accumulation to petroleum hydrocarbon of alkali
Removal amount continues to reduce.Soil Petroleum Hydrocarbon total removal rate is 48.69%.
Fig. 5 is soil Petroleum Hydrocarbon concentration distribution in the 180th day, the reparation of 5cm and 10cm that turn under different ploughed depth
Effect is substantially better than other two kinds of situations.Along with the increase of ploughed depth, microbial degradation amount improves, and root system effect is relatively
Weak, in general, in the case of initial concentration is 2000mg/kg, 5cm is best ploughed depth.Turn over this
Under the conditions of Genging, petroleum hydrocarbon contaminant is under the effect of root system of plant for a long time, it is possible to make root system of plant play maximum effect.
In a word, the key factor affecting petroleum hydrocarbon degradation effect includes petroleum hydrocarbon contaminated concentration, soil moisture content and Polluted Soil
Earth ploughed depth etc..Simulation can rationally determine the Optimal Parameters of initial contamination concentration, irrigation and ploughed depth, determines plant
With microbial degradation ability, the soil restoring technology proposing to be suitable for local feature optimizes application process, improves soil pollutant
Degraded and repairing effect.
Claims (1)
1. the optimizing application method of the plant-microorganism recovery technique being suitable to local soil, it is characterised in that the party
Method following steps:
Step (1), the natural precipitation amount determining soil remediation locality and depth of soil, moisture content parameter；
Step (2), set up soil remediation model, including following four kinds:
Soil remediation model one, the selection soil lower boundary degree of depth, if petroleum hydrocarbon concentration has exceeded the pollution background value preset,
Representing in the case of high concentration is petroleum hydrocarbon contaminated, lower boundary degree of depth soil below is contaminated, deep in conjunction with further checking
The layer petroleum hydrocarbon contaminated degree of soil and scope, use soil to blend the reclamation activities reducing pollutant levels；
Soil remediation model two, when the half that petroleum hydrocarbon peak concentration reduces to initial concentration being detected, show existing
Under repairing condition, low concentration is the most petroleum hydrocarbon contaminated reaches repairing effect；
Soil remediation model three, the local condition of raining of combination, for reaching petroleum hydrocarbon degradation rate, use and adjust irrigation volume and set
The reclamation activities of meter irrigation method；
Soil remediation model four, the local soil characteristic of combination, for reaching the microbial degradation amount optimized, use and adjust soil
The reclamation activities of ploughed depth；
Step (3), according to local feature, in conjunction with above-mentioned soil remediation model, recovery technique under simulation different condition
Application effect；
Step (4), selection adapt to the soil remediation model of local condition, use the reclamation activities being suitable for；
Step (5), rationally determine the Optimal Parameters of initial contamination concentration, irrigation and ploughed depth, determine plant and micro-
Biodegradability, implements the recovery technique scheme optimized.
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| CN108687131A (en) * | 2017-12-07 | 2018-10-23 | 苏州市环科环保技术发展有限公司 | Chemical pollution site remediation method |
| CN109108063A (en) * | 2018-10-29 | 2019-01-01 | 中国石油大学(华东) | A kind of biology expedient of tillage oily sludge soil remediation construction technology |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106269824A (en) * | 2016-09-22 | 2017-01-04 | 北京新源环境有限公司 | Plant bionic repairs the method for industry combined contamination soil |
| CN108687131A (en) * | 2017-12-07 | 2018-10-23 | 苏州市环科环保技术发展有限公司 | Chemical pollution site remediation method |
| CN109108063A (en) * | 2018-10-29 | 2019-01-01 | 中国石油大学(华东) | A kind of biology expedient of tillage oily sludge soil remediation construction technology |
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