CN113714275A - Soil layer multistage inoculation microorganism regulation and control method - Google Patents
Soil layer multistage inoculation microorganism regulation and control method Download PDFInfo
- Publication number
- CN113714275A CN113714275A CN202010975037.1A CN202010975037A CN113714275A CN 113714275 A CN113714275 A CN 113714275A CN 202010975037 A CN202010975037 A CN 202010975037A CN 113714275 A CN113714275 A CN 113714275A
- Authority
- CN
- China
- Prior art keywords
- soil
- plant
- pyrolytic carbon
- microbial inoculum
- carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002689 soil Substances 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 28
- 244000005700 microbiome Species 0.000 title claims abstract description 19
- 238000011081 inoculation Methods 0.000 title claims abstract description 13
- 241000196324 Embryophyta Species 0.000 claims description 40
- 239000002296 pyrolytic carbon Substances 0.000 claims description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 29
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 28
- 229910052799 carbon Inorganic materials 0.000 claims description 28
- 238000000197 pyrolysis Methods 0.000 claims description 28
- 239000002068 microbial inoculum Substances 0.000 claims description 24
- 230000001580 bacterial effect Effects 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 14
- 239000002028 Biomass Substances 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 10
- 235000004443 Ricinus communis Nutrition 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 7
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 6
- 240000000528 Ricinus communis Species 0.000 claims description 6
- 240000008564 Boehmeria nivea Species 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 238000011065 in-situ storage Methods 0.000 claims description 5
- 230000000813 microbial effect Effects 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 241000206596 Halomonas Species 0.000 claims description 3
- 239000004005 microsphere Substances 0.000 claims description 3
- 230000000243 photosynthetic effect Effects 0.000 claims description 3
- 238000007599 discharging Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000896533 Gliocladium Species 0.000 claims 1
- 238000007600 charging Methods 0.000 claims 1
- 239000008188 pellet Substances 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 13
- 239000005416 organic matter Substances 0.000 abstract 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 14
- 239000007789 gas Substances 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- 239000003921 oil Substances 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000001569 carbon dioxide Substances 0.000 description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 description 7
- 239000003344 environmental pollutant Substances 0.000 description 7
- 239000008187 granular material Substances 0.000 description 7
- 239000010410 layer Substances 0.000 description 7
- 231100000719 pollutant Toxicity 0.000 description 7
- 238000001035 drying Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000003208 petroleum Substances 0.000 description 6
- 241000235503 Glomus Species 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 239000010902 straw Substances 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 4
- 239000000292 calcium oxide Substances 0.000 description 4
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 239000003209 petroleum derivative Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 3
- 241000209082 Lolium Species 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 3
- 240000006394 Sorghum bicolor Species 0.000 description 3
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000011261 inert gas Substances 0.000 description 3
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010926 purge Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000002344 surface layer Substances 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 229910052785 arsenic Inorganic materials 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000000571 coke Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 239000000112 cooling gas Substances 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 229910052745 lead Inorganic materials 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000003900 soil pollution Methods 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 240000001398 Typha domingensis Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- JXBAVRIYDKLCOE-UHFFFAOYSA-N [C].[P] Chemical compound [C].[P] JXBAVRIYDKLCOE-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004939 coking Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000003546 flue gas Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- -1 microspheres Chemical compound 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 125000003367 polycyclic group Chemical group 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
- 239000002916 wood waste Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Processing Of Solid Wastes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention discloses a soil layer multistage inoculation microorganism regulation and control method, which comprises the steps of applying one or more first microorganisms to polluted soil and planting restorative plants, and optionally inoculating other microorganisms. The soil layer multistage inoculation microorganism regulation and control method can efficiently, permanently and irreversibly remove heavy metal and organic matter pollution in soil, and cannot damage the soil structure.
Description
Technical Field
The invention relates to a soil layer multistage inoculation microorganism regulation and control method, and belongs to the technical field of soil restoration and improvement.
Background
During the process of oil extraction, storage, transportation and petrochemical production, leakage events are often accompanied, causing a large amount of oil to enter soil to cause environmental pollution. Aromatic substances in petroleum pollutants have high toxicity to human beings and animals, and particularly aromatic hydrocarbons represented by polycyclic and tricyclic rings. Polycyclic aromatic hydrocarbons (polycyclic aromatic hydrocarbons) are toxic organic pollutants containing two or more benzene rings widely distributed and stably existing in natural environment, leakage of petroleum and petroleum products is one of main sources of the pollutants, and after some petroleum hydrocarbons enter animal bodies, even mammals and human beings are injured by cancer, teratogenesis and mutation.
The petroleum substances enter the soil, which can cause the change of the physicochemical properties of the soil, such as blocking the soil pores, changing the composition and structure of soil organic matters, causing the change of carbon-nitrogen (C/N) and carbon-phosphorus ratio (C/P) of the soil organic matters, even destroying the original carbon, nitrogen and phosphorus ratio in the soil, and causing the change of soil microbial communities and microbial communities. Organic pollution easily causes soil hardening, changes the pH value and destroys the structure and the composition of the soil.
Heavy metal is another pollution source of soil, according to statistics, the area of the polluted soil in China currently reaches more than 5000 ten thousand mu, various heavy metal elements in the soil exceed the standard in different degrees, and due to the characteristics of poor mobility, long residence time, difficulty in degradation and the like of the heavy metal pollutants in the soil, the heavy metal pollutants have great influence on the self-circulation capability of the soil, and are easy to influence the health of human beings through media such as water, plants and the like.
Many polluted soils often have organic pollution and heavy metal pollution condition simultaneously, it becomes urgent to explore soil restoration technical method and system equipment for comprehensively regulating and controlling organic pollution and heavy metal pollution, and the current adopted restoration technology generally has the problems of low restoration efficiency, high cost, easy secondary pollution, easy damage to soil structure and the like.
Disclosure of Invention
In order to overcome the above problems, the inventors have studied and found that: the method has the advantages that the polluted soil is repaired by inoculating the microbial inoculum in multiple stages, appropriate plants are planted, pollutants in the soil can be removed efficiently, permanently and irreversibly, and the soil structure cannot be damaged.
Specifically, the invention provides a soil layer multistage inoculation microorganism regulation method, which comprises applying one or more first microorganisms to polluted soil and planting plants, and optionally inoculating other microorganisms.
According to the invention, the strain of the first microorganism is selected from saccharomycetes, photosynthetic bacteria, halopmonas and pseudomonas aeruginosa, preferably, the strain is a multi-strain bacterial solution formed by compounding saccharomycetes, halopmonas and pseudomonas aeruginosa, and more preferably, the mass ratio of each strain is (1-5): (1-4): 1, more preferably (2-4): (2-3) 1.
According to the invention, the microbial inoculum is applied together with a biomass carbon source, preferably a solid biomass carbon source, preferably pyrolytic carbon, which can be granular, preferably mixed with other granular materials, such as inorganic granular materials, and is preferably soaked in the bacterial liquid.
According to the invention, the mixing is carried out in situ in a pyrolysis furnace, and the calcium carbonate-doped pyrolytic carbon is formed after feeding, pyrolysis, discharge, transport and cooling, and is preferably pressed into microspheres, strips or rods.
In the invention, the pyrolytic carbon is soaked in the bacterial liquid for several hours, for example, 0.5 to 5 hours, and preferably, the pyrolytic carbon is soaked before being applied to soil to obtain the pyrolytic carbon microbial inoculum compound.
In the present invention, the restorative plant is a crop, a commercial crop or other herbaceous plant, preferably rice, wheat, ryegrass, sorghum, corn, castor-oil plant, ramie, more preferably castor-oil plant, ramie.
In the invention, after the reparative plants are planted, another microbial inoculum, which comprises phanerochaete chrysosporium and glomus parvifolia, is applied at a depth of 10-30cm, preferably 12-18cm, from the surface layer of the soil, and is preferably a spore suspension.
The invention has the advantages that:
1. according to the soil layer multistage inoculation microorganism regulation and control method provided by the invention, multiple repair and regulation and control effects of the microbial inoculum and plants are combined, heavy metals and organic pollutants in soil are effectively reduced, and in-situ repair can be realized;
2. according to the regulation and control method provided by the invention, the plant is planted and repaired, so that the environment can be beautified, the petroleum hydrocarbon pollution concentration can be reduced, the soil quality of the polluted soil can be effectively improved, the pollutants are prevented from entering a food chain again, the secondary pollution of the repaired soil is reduced to the maximum extent, the cost is low, the influence on the environment is small, the ground surface can be stabilized for a long time, the pollutants in the atmosphere and the water body around the polluted soil are eliminated while the soil pollution is eliminated, the ecological environment is favorably improved, and the biological diversity of the soil is improved;
3. according to the regulation and control method provided by the invention, the bio-based carbon source contains a certain organic component, has the effects of improving the physical structure of soil and increasing the soil fertility, is applied in the early stage of planting plants or crops, provides good loading and solidification effects on the microbial inoculum, and can effectively reduce the influence of petroleum pollution on the growth of the plants under the combined action of the microbial inoculum and the microbial inoculum, adsorb and fix heavy metals in the soil, provide favorable conditions for the growth of the plants, and is a safe, environment-friendly, efficient, economic and practical method for repairing the petroleum-heavy metal polluted soil.
Detailed Description
The present invention will be described in further detail below with reference to preferred embodiments and examples. The features and advantages of the present invention will become more apparent from the description.
The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
According to the present invention, there is provided a method for regulating the microbial inoculation of a soil layer at multiple stages, comprising applying one or more first microorganisms to contaminated soil and growing plants, and optionally inoculating other microorganisms.
According to the invention, the species of the first microorganism may be one species or a plurality of species, for example selected from yeasts, photosynthetic bacteria, halopmonas, and pseudomonas aeruginosa, all commercially available strains.
The strain is activated by conventional method and then cultured in an enlarged scale, preferably at a thallus concentration of 107～109And (3) obtaining bacterial suspension by using each/mL of liquid strain, and further culturing the bacterial suspension to prepare a microbial inoculum which can be a microbial inoculum of one strain or a composite microbial inoculum of multiple strains.
According to the preferred embodiment of the invention, the microbial inoculum is formed by compounding saccharomycetes, halomonas and pseudomonas aeruginosa, and the mass ratio of each strain is (1-5): (1-4): 1, more preferably (2-4): (2-3): 1, in terms of volume ratio when the bacterial suspension is weighed.
According to the invention, the composite bacterial liquid with the types and the proportion is beneficial to improving the remediation efficiency and the remediation quality of the polycyclic aromatic hydrocarbon organic contaminated soil, and meanwhile, the fertility of the soil can be improved, and the absorption of mineral elements by plants is facilitated.
According to the invention, the microbial inoculum and biomass carbon source pyrolytic carbon are applied together to pyrolytic carbon. The biomass carbon source is preferably a solid material, and plays roles in solidifying, dispersing and protecting the microbial inoculum. Therefore, the solid biomass carbon source is soaked in the bacterial liquid to endow the carbon source with biological activity, so that the soil optimization effect is further improved.
In the present invention, the biomass carbon source is preferably pyrolytic carbon, which may be in the form of granules, preferably mixed with other granules, such as inorganic granules. The mixing may be physical mixing of the pyrolytic carbon and the inorganic granules, respectively, in a finished product, or in-situ mixing during the preparation process.
According to the invention, the in-situ mixing is completed in a pyrolysis device, the pyrolysis device comprises a pyrolysis furnace, a plant carbon source and an additive enter the pyrolysis furnace together, generated semi-coke and primary pyrolysis gas containing carbon dioxide react with calcium oxide in the additive, the final pyrolysis gas is discharged through a pipeline, calcium carbonate and pyrolysis carbon of reaction products are discharged and sent to a cooling zone to contact with cooling gas and conduct heat exchange, warm flue gas passes through a cyclone separator and is converged with sweeping gas entering from a gas supply pipeline and returns to the pyrolysis furnace together, the feeding, pyrolysis, discharging, conveying and cooling of pyrolysis carbon are completed, and closed circulation of the gas is realized.
In the invention, the plant carbon source is a biomass material or material capable of providing carbon elements, and is selected from herbaceous plants or woody plants, the herbaceous plants can be harvested or harvested terrestrial or aquatic crops or plants, such as rice straws, sorghum straws and maize straws, harvested materials of wheat, ryegrass, castor-oil plants and ramie, reeds or cattails, and the woody plants can be forestry biomass, such as roots, branches and leaves of various trees, branches and leaves of shrubs, or other forestry waste or wood waste. More preferably, cut or harvested reparative plants and their waste are used.
According to the present invention, the plant carbon source is subjected to appropriate treatments including drying and pulverization. The plant carbon source is dried to remove moisture, such as by sun drying or natural air drying, preferably the plant carbon source is air dried so that the plant moisture content is 10% to 20%, and then chopped. Before drying the plants, they are washed, preferably with deionized water, and then soaked in dilute hydrochloric acid.
In the present invention, the plant carbon source is pyrolyzed in a pyrolysis apparatus, such as a pyrolysis furnace, at a medium-low temperature. The plant carbon source may be directly pyrolyzed, preferably by purging with an inert gas such as nitrogen or carbon dioxide gas in advance, and then heating with an external heat source, and the temperature of the pyrolysis furnace is raised to 200 ℃ or more, more preferably 300 to 600 ℃. The low temperature is not favorable for volatilization and stripping of low-boiling-point organic matters, and the higher temperature is easy to cause excessive coking or complete carbonization. The process is maintained for several tens of minutes to several hours while maintaining the circulation of nitrogen to carry away the gaseous products generated by pyrolysis while maintaining an oxygen-deficient environment.
The pyrolysis is mainly carried out in three main stages of dehydration drying, preheating and volatile component separation. Dehydration drying is to remove internal crystal water; then entering a short preheating stage, and continuously increasing the number of active structures of raw material molecules; volatile components are separated and separated out gradually after preheating, light hydrocarbon compounds are cracked and separated out continuously to generate carbon monoxide, methane, hydrogen, carbon dioxide and the like, and the proportion of the residual fixed carbon is increased gradually. The obtained crystal water, the generated volatile gas and most of tar steam (collectively called pyrolysis gas) can be discharged out of the pyrolysis furnace, the pyrolysis gas can be used as heating fuel, and volatile gas liquid can be separated out through condensation, namely pyrolysis oil.
Preferably, calcium oxide and/or calcium carbonate is added to the pyrolysis furnace simultaneously with or after the addition of the plant carbon source, prior to separation of the pyrolysed carbon particles.
The reacted material is discharged to a cooling area, can be naturally cooled, and preferably, the incandescent pyrolytic carbon is directly cooled by inert gas to obtain pyrolytic carbon particles and hot gas. The cooling gas is preferably the same inert gas used for purging, and may be nitrogen, carbon dioxide, or a mixture of both, with an amount of carbon dioxide that aids in the formation of calcium carbonate upon pyrolysis.
According to the invention, the solid separated after cooling is a pyrolytic carbon, in granular form, possibly comprising, in addition to the pyrolytic carbon particles, calcium carbonate, in which case a part of it originates from the added calcium carbonate and another part from calcium carbonate formed by calcium oxide and carbon dioxide. The addition of the calcified substance can reduce the content of carbon dioxide in the pyrolysis gas, can also mix the pyrolytic carbon particles with calcium carbonate together, increases the density of particle materials, promotes the dispersion of the pyrolytic carbon, avoids agglomeration, and is very favorable for the load solidification of bacteria liquid, because the uniformly distributed carrier material is compact and porous.
According to the invention, the produced pyrolytic carbon contains organic substances with higher boiling points, is not large in density, low in mechanical strength, easier to crush than biomass raw materials, consumes less energy for crushing, and is preferably crushed for better forming, and can be directly pressed into a certain shape, preferably pressed into a certain shape together with calcium carbonate, such as microspheres, strips or rods, for example, with a particle size or a cross-sectional diameter of 0.5mm to 10mm, preferably 1mm to 5mm, for example 2mm to 4 mm.
In a preferred embodiment of the invention, the pyrolytic carbon and the calcium carbonate are pressed together with the pyrolytic oil, so that the pyrolytic oil can play a role of an adhesive on one hand, carbon powder is easy to adhere, the condition that a formed product is loose is avoided, meanwhile, the pyrolytic oil is a pyrolysis product of the raw material, no additional material is needed, the cost is reduced, and on the other hand, heavy carbon organic matters in the pyrolytic oil are slowly decomposed or degraded in the soil improvement process, and a carbon source or nutrient is continuously provided, so that the effect of fertilizer holding and slow release is generated, and the effective repair of the soil is promoted.
According to the present invention, in order to inoculate the bacterial strain on the doped pyrolytic carbon in multiple stages, the pyrolytic carbon is soaked in the bacterial liquid, and the soaking time can be several hours, for example, 0.5 to 5 hours, preferably 2 to 3 hours, according to actual needs.
And then, taking out the soaked pyrolytic carbon, and culturing the pyrolytic carbon at a preset temperature, preferably 30-35 ℃ for a period of time, preferably 5-30 h, preferably 12-18 h to obtain the doped pyrolytic carbon with bioactivity, wherein calcium carbonate (the sedimentation volume is about 2.4-2.8mL/g) is lighter than heavy calcium carbonate (the sedimentation volume is about 1.1-1.9mL/g) produced by a mechanical method, so that the doped pyrolytic carbon is light calcium carbonate and is used as a microbial carrier together with the pyrolytic carbon to provide space for attachment of loaded microbes, on one hand, the pyrolytic carbon can effectively improve the activity of the microbes and the capability of regulating and controlling soil pollution, and on the other hand, the pyrolytic carbon can well protect strains.
According to the invention, the prepared pyrolytic carbon microbial inoculum compound is applied to the polluted soil to be regulated, and the addition amount of the compound is 1-3% by dry weight of the soil. For this purpose, the soil is deep-ploughed, for example by 10 to 30cm, preferably 20 to 25 cm. Then left for days to weeks, preferably for a period of one week.
According to the invention, a restorative plant is planted on the soil after the above treatment, and optionally other microorganisms are applied, for example in the form of a microbial inoculum.
According to the invention, the reparative plant can be a crop, a commercial crop, or other herbaceous plants.
According to the invention, preferably, rice, wheat, ryegrass, sorghum, corn, castor-oil plant, ramie and the like can be planted according to the conditions of regions, water conservancy and the like.
According to an optional embodiment of the invention, another inoculum, comprising Phanerochaete chrysosporium and glomus parvulus, preferably a spore suspension, is applied several days after planting the restorative plant, depending on the plant type and growth, for example within 5 to 30 days. In the present invention, Phanerochaete chrysosporium and glomus parvifolium are commercially available, and the spore suspension can be prepared by a method generally used in the art. In the present invention, the application position of the microbial inoculum varies depending on the plant species, for example, a position 10 to 30cm, preferably 12 to 18cm deep from the surface layer of the soil. The seed can be inoculated for multiple times, such as layered inoculation, preferably, one layer is inoculated for every 1-1.5 cm of thickness, and 1-4 layers can be inoculated.
According to the invention, the inoculation amount of the microbial inoculum is 0.8-1.5 g/m2Preferably 1.0 to 1.2g/m2The application can be performed by flowing irrigation or pumping.
Preferably, the planted restorative plants are subjected to normal field management including watering, fertilizing, weeding, and harvesting on time.
Examples
The present invention is further described below by way of specific examples, which are merely exemplary and do not limit the scope of the present invention in any way.
Example 1
Washing corn stalk with water, soaking in 0.01M dilute hydrochloric acid for 2 hr, air drying to water content of 15-18%, and cutting. Introducing nitrogen into a pyrolysis furnace for purging, then adding straws and a calcium mixture (the weight ratio of calcium oxide to calcium carbonate is 2: 1) which accounts for one fifth of the weight of the straws through a feeding port, heating to 450-550 ℃, and carrying out heat preservation reaction for 2 hours while keeping the circulation of nitrogen.
And then cooling to room temperature to obtain pyrolytic carbon, crushing the pyrolytic carbon, and pressing the pyrolytic carbon and the antipyretic oil obtained by condensing the pyrolytic gas into granules with the particle size of 2-4 mm.
Culturing yeast, halopmonas and Pseudomonas aeruginosa in an enlarged manner to obtain strain with a concentration of 109Mixing liquid strains per mL according to the mass ratio of the strains of 3.2:2.5:1, soaking the semi-coke carbon particles in the bacterial liquid for 4 hours, and culturing in a constant temperature incubator at 30 ℃ for 12 hours to obtain the pyrolytic carbon-microbial inoculum compound.
Selecting an experimental soil area polluted by heavy metal and petroleum, dividing the samples, wherein the length and the width of each sample are 5m, ridges with the width of 0.8m are reserved between every two samples, and each sample is provided with 5 sampling points according to a diagonal principle. And (4) adopting 0-25cm soil on the surface layer of the test field, cooling in the shade, ventilating and drying, and removing impurities. The soil pH is measured to be 7.7, the petroleum hydrocarbon content is 830.4mg/kg, and the concentrations of heavy metals Zn, Pb, Cd, Cu, Hg and As are 475.2, 320.6, 95.4, 357.2, 15.6 and 97.5mg/kg respectively.
Adding the soil into the polluted soil according to the addition amount of 1.5 percent of the dry weight of the soil, deeply turning for 20-25cm, and then placing for a week.
Then planting castor-oil plant in the soil, after 15 days of planting, irrigating a microbial inoculum which is spore suspension obtained after activation of phanerochaete chrysosporium and glomus parvulus (the concentration of the spore suspension of the phanerochaete chrysosporium is 2.5 multiplied by 10)6Per mL, concentration of the spore suspension of glomus minor was 3.2X 106one/mL, the volume ratio of the two mixed is 4:8), the inoculation amount is 1.1g/m2The inoculation is divided into 2 times. After the castor is ripe, harvesting the castor.
After the soil in the experimental area is treated by the method, sampling is carried out, and the analysis and determination results are as follows: the pH value of the soil is reduced to 7.4, the content of petroleum hydrocarbon is 380.4mg/kg, the concentrations of heavy metals Zn, Pb, Cd, Cu, Hg and As are 175.8, 69.5, 0.81, 91.3, 1.13 and 25.8mg/kg respectively, and the soil meets or basically meets the national soil environment quality standard.
The invention has been described in detail with reference to specific embodiments and illustrative examples, but the description is not intended to be construed in a limiting sense. Those skilled in the art will appreciate that various equivalent substitutions, modifications or improvements may be made to the technical solution of the present invention and its embodiments without departing from the spirit and scope of the present invention, which fall within the scope of the present invention.
Claims (10)
1. A method for regulating the microbial inoculation of a soil layer at multiple stages, comprising applying one or more first microorganisms to the contaminated soil and growing a remediating plant, and optionally inoculating additional microorganisms.
2. The method according to claim 1, wherein the species of the first microorganism is selected from yeast, photosynthetic bacteria, halomonas, and pseudomonas aeruginosa, preferably a multi-species bacterial solution compounded from yeast, halomonas, and pseudomonas aeruginosa.
3. The method according to claim 2, wherein the microbial inoculum is co-applied with a biomass carbon source, preferably by soaking the biomass carbon source in a bacterial solution.
4. A method according to claim 3, characterized in that the biomass carbon source is pyrolytic carbon, preferably in granular form, more preferably in admixture with other pellets.
5. The method according to claim 4, wherein the mixing is in-situ mixing during the preparation process, preferably in a pyrolysis furnace, and the calcium carbonate doped pyrolytic carbon is formed after charging, pyrolysis, discharging, transporting and cooling.
6. The method of claim 4, wherein the doped pyrolytic carbon is pressed into microspheres, strips, or rods.
7. The method according to any one of claims 4 to 6, wherein the pyrolytic carbon is soaked in the bacterial solution for several hours, such as 0.5 to 5 hours, preferably before being applied to soil, to obtain the pyrolytic carbon microbial inoculum compound.
8. The method according to one of claims 1 to 7, the restorative plant being a crop, a cash crop or another herbaceous plant, preferably castor-oil plant, ramie.
9. The method according to any one of claims 1 to 8, wherein another microbial inoculum is applied 10 to 30cm, preferably 12 to 18cm, from the surface of the soil after the planting of the restorative plant.
10. The method according to claim 9, wherein the microbial inoculum is a spore suspension of Phanerochaete chrysosporium and Gliocladium parvum.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2020104455459 | 2020-05-24 | ||
| CN202010445545 | 2020-05-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113714275A true CN113714275A (en) | 2021-11-30 |
Family
ID=78672266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010975037.1A Pending CN113714275A (en) | 2020-05-24 | 2020-09-16 | Soil layer multistage inoculation microorganism regulation and control method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113714275A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115532809A (en) * | 2022-09-27 | 2022-12-30 | 西南科技大学 | Process for treating heavy metal pollution of soil |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101104177A (en) * | 2007-08-01 | 2008-01-16 | 中国石油化工股份有限公司 | In-situ biological repairing method for biomass intensified petroleum contaminative soil |
| CN101172286A (en) * | 2007-08-02 | 2008-05-07 | 中国石油化工股份有限公司 | Biology in situ renovation method for biomass strengthen mix contaminated soil of stone oil-salt |
| CN102601102A (en) * | 2012-02-09 | 2012-07-25 | 中国科学院生态环境研究中心 | Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium |
| CN105985784A (en) * | 2016-02-24 | 2016-10-05 | 凤阳徽亨商贸有限公司 | Heavy metal repairing agent for improving ion exchange capacity of soil |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
| CN108941191A (en) * | 2018-07-03 | 2018-12-07 | 上海松沅环境修复技术有限公司 | A kind of repairing method of microorganism of oil-polluted soils |
| CN109926445A (en) * | 2017-12-19 | 2019-06-25 | 国家电投集团远达环保工程有限公司重庆科技分公司 | A kind of soil remediation method |
| CN110283599A (en) * | 2019-07-09 | 2019-09-27 | 郑州轻工业学院 | A kind of heavy-metal contaminated soil renovation agent and preparation method thereof |
-
2020
- 2020-09-16 CN CN202010975037.1A patent/CN113714275A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101104177A (en) * | 2007-08-01 | 2008-01-16 | 中国石油化工股份有限公司 | In-situ biological repairing method for biomass intensified petroleum contaminative soil |
| CN101172286A (en) * | 2007-08-02 | 2008-05-07 | 中国石油化工股份有限公司 | Biology in situ renovation method for biomass strengthen mix contaminated soil of stone oil-salt |
| CN102601102A (en) * | 2012-02-09 | 2012-07-25 | 中国科学院生态环境研究中心 | Method for repairing high-concentration oil-polluted soil through mycorrhizal fungi-plant-degradation bacterium |
| CN105985784A (en) * | 2016-02-24 | 2016-10-05 | 凤阳徽亨商贸有限公司 | Heavy metal repairing agent for improving ion exchange capacity of soil |
| CN107321780A (en) * | 2017-09-06 | 2017-11-07 | 杭州智仁建筑工程有限公司 | A kind of phytomicroorganism joins the method for restoration of soil polluted by heavy metal |
| CN109926445A (en) * | 2017-12-19 | 2019-06-25 | 国家电投集团远达环保工程有限公司重庆科技分公司 | A kind of soil remediation method |
| CN108941191A (en) * | 2018-07-03 | 2018-12-07 | 上海松沅环境修复技术有限公司 | A kind of repairing method of microorganism of oil-polluted soils |
| CN110283599A (en) * | 2019-07-09 | 2019-09-27 | 郑州轻工业学院 | A kind of heavy-metal contaminated soil renovation agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李广贺等: "《污染场地环境风险评价与修复技术体系》", 31 January 2010, 中国环境科学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115532809A (en) * | 2022-09-27 | 2022-12-30 | 西南科技大学 | Process for treating heavy metal pollution of soil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10947167B2 (en) | Biochar | |
| US20200079700A1 (en) | Biochar Compositions and Methods of Use Thereof | |
| Sukartono et al. | Soil fertility status, nutrient uptake, and maize (Zea mays L.) yield following biochar and cattle manure application on sandy soils of Lombok, Indonesia | |
| KR102018188B1 (en) | Hybrid biochar and method of preparing for the same | |
| Utomo et al. | Soil fertility status, nutrient uptake, and maize (Zea mays L.) yield following biochar and cattle manure application on sandy soils of Lombok, Indonesia | |
| Gurtler et al. | Inactivation of E. coli O157: H7 in cultivable soil by fast and slow pyrolysis-generated biochar | |
| CN110918055A (en) | Composite material for efficiently removing phosphorus in wastewater, preparation method and application thereof | |
| KR102021771B1 (en) | Charcoal production or production for improving soil environmental using effective microorganisms and manufacturing method | |
| Saxena et al. | Conversion of biomass waste into biochar and the effect on mung bean crop production | |
| CN112374941A (en) | Soil remediation agent prepared from crop straws and biochar | |
| Toscano et al. | Olive mill by-products management | |
| CN113860931B (en) | Aerobic composting method for removing antibiotics in feces by using chicken manure biochar and application of chicken manure biochar in removing antibiotics | |
| CN113714275A (en) | Soil layer multistage inoculation microorganism regulation and control method | |
| Tan et al. | Biochar amendment to lead‐contaminated soil: Effects on fluorescein diacetate hydrolytic activity and phytotoxicity to rice | |
| JP2023507995A (en) | Method of using granular pyrolytic carbon for soil improvement | |
| CN113714274B (en) | Plunger pump for microbial soil layer dispersion mixing inoculation | |
| CN113698937B (en) | Humic acid-earthworm-semicoke soil improvement technical method | |
| CN113699055B (en) | Technical equipment for emulsifying and activating microbial inoculum | |
| CN113293004A (en) | Oil sludge pyrolytic carbon-based soil conditioner and preparation method thereof | |
| CN113714270A (en) | Remediation device and remediation method for soil radioactive element pollution | |
| CN113698936B (en) | Technical method for emulsifying and activating bacteria agent | |
| CN111533615A (en) | Composition for repairing high-oil-content clay and repairing method thereof | |
| Wedayani et al. | Utilization of banana waste biochar to reduce heavy metal contamination in soil and maize plants | |
| CN113698938A (en) | Humic acid-earthworm-semicoke soil improvement technical method | |
| Suliman et al. | 8. Composting in the Presence of Biochar and Impact of the Resulting Product as a Soil Amendment: A Review |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Xiang Inventor after: Yu Hong Inventor after: Li Yanping Inventor after: Xi Beidou Inventor after: Guo Kexin Inventor after: Wang Lei Inventor after: Yan Zheng Inventor after: Liu Jin Inventor after: Wang Yangyang Inventor after: Hu Yanbin Inventor after: Wang Jinsheng Inventor after: Liu Hui Inventor after: Cui Dongyu Inventor before: Li Xiang Inventor before: Guo Kexin Inventor before: Hu Yanbin Inventor before: Wang Lei Inventor before: Xi Beidou Inventor before: Wang Jinsheng Inventor before: Yan Zheng Inventor before: Liu Hui Inventor before: Cui Dongyu Inventor before: Yu Hong Inventor before: Li Yanping |
|
| CB03 | Change of inventor or designer information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211130 |
|
| RJ01 | Rejection of invention patent application after publication |