CN108941191A - A kind of repairing method of microorganism of oil-polluted soils - Google Patents
A kind of repairing method of microorganism of oil-polluted soils Download PDFInfo
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- CN108941191A CN108941191A CN201810718401.9A CN201810718401A CN108941191A CN 108941191 A CN108941191 A CN 108941191A CN 201810718401 A CN201810718401 A CN 201810718401A CN 108941191 A CN108941191 A CN 108941191A
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- oil
- polluted soils
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
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- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
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- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of repairing method of microorganism of oil-polluted soils, it is related to the recovery technique field of oil-polluted soils.It the steps include: that oil-polluted soils are collected into isolation reparation place to be repaired, while the contaminated soil of collection being dried and is pulverized, to remove volatile hydrocarbon；It prepares complex micro organism fungicide: after the completion of Phanerochaete chrysosporium, bacillus licheniformis, pseudomonas aeruginosa and Achromobacter xylosoxidans culture, uniformly being mixed according to the ratio of the 0.5-1:0.5-1:2-3:1-2 of bacterial strain concentration；Composite bacteria agent, biomass castoff, surfactant are proportionally added separately to soil, and uniformly mixed, 45-60 days sample detection repairing effects are reacted.Operation of the present invention is simple, strong operability, and petroleum hydrocarbon degradation rate is high, and at low cost, energy conservation and environmental protection.
Description
Technical field
The present invention relates to the recovery technique fields of oil-polluted soils, and in particular to a kind of oil-polluted soils it is micro-
Biological renovation method.
Background technique
China is the big country of production and consumption petroleum, however, during petroleum and petrochemical industry relevant enterprise production and transportation not
Occur the leakage of petroleum avoidablely, finally pollutes Soil Surrounding, the soil of oil pollution big, organic carbon with contaminated area
The features such as content is high, processing difficulty is big and toxicity is high, leads to the decline of soil quality and the imbalance of soil environment normal function.
Currently, common oil-polluted soils restorative procedure has: peripheral doses method, chemical repair method, phytoremediation
Method and repairing method of microorganism etc..And microorganism remediation technology is the extension of traditional biological processing method, has and does not cause two
It is secondary pollution, it is low-cost, do not destroy soil texture, administer area it is big, it is various informative flexibly etc. unique advantages, have been obtained
Domestic and international expert's is widely recognized as, and is based on this, the repairing method of microorganism for designing a kind of oil-polluted soils is particularly necessary.
Summary of the invention
In view of the shortcomings of the prior art, object of the present invention is to be to provide a kind of microorganism of oil-polluted soils
Restorative procedure, easy to operate, strong operability, petroleum hydrocarbon degradation rate is high, and at low cost, energy conservation and environmental protection, use easy to spread.
To achieve the goals above, the present invention is to realize by the following technical solutions: a kind of oil-polluted soils
Repairing method of microorganism the steps include:
(1) oil-polluted soils are collected into isolation reparation place to repair, while the contaminated soil of collection are dried and is pulverized,
To remove volatile hydrocarbon；
(2) complex micro organism fungicide is prepared: Phanerochaete chrysosporium, bacillus licheniformis, pseudomonas aeruginosa and xylose oxidation
After the completion of achromobacter culture, uniformly mixed according to the ratio of the 0.5-1:0.5-1:2-3:1-2 of bacterial strain concentration；
(3) composite bacteria agent, biomass castoff, surfactant are proportionally added separately to the soil in step (1), and
45-60 days sample detection repairing effects are reacted in uniformly mixing.
Preferably, the mass concentration range of the pollution petroleum hydrocarbon of oil-polluted soils is 2%- in the step (1)
12%。
Preferably, complex micro organism fungicide culture completion requires bacterial strain concentration to reach 10 in the step (2)9cfu/
ML, soil connect bacterium amount higher than 108cfu/kg。
Preferably, in the step (2) Phanerochaete chrysosporium activation culture based formulas are as follows: potato 20%, Portugal
Grape sugar 2%, agar 1.5%-2.0%, pH are natural；Fermentative medium formula are as follows: potato 0.2%, glucose 2.0%, KH2PO4
0.3%、MgSO4·7H2O 0.15%、FeSO4·7H2O 0.1mg/L、CuSO4·5H2O 0.2mg/L, vitamin B1 8mg/L.
Preferably, bacillus licheniformis in the step (2), pseudomonas aeruginosa and Achromobacter xylosoxidans
Activation medium composition: beef extract 0.5%, peptone 1%, NaCl 0.5%, pH 7.0-7.2；Fermentation medium composition: hydrolysis
Sugared 2.5%, corn pulp 2.5%, KH2PO4 0.15%、MgSO40.04%, urea 0.4%, pH 6.8-7.0.
Preferably, biomass castoff is using one in stalk, waste wood, peanut shell, withered grass in the step (3)
Kind is a variety of.
Preferably, in the step (3) surfactant using rhamnolipid, humic acid, Triton X-100,
One of Tween80, the additive amount of surfactant are 0.5 ‰ -1.5 ‰.
Preferably, controlling 15-30 DEG C of reaction temperature in the step (3), moisture content is in holding reaction system
20%-30%, nitrogen: phosphorus mass ratio is 8-12:1, adjusts pH6.0-8.0, keeps the bacterium of addition in 3-7 days, and periodically turn over ventilation,
It controls the reaction system prepared and banks up into stack.
Beneficial effects of the present invention: it is repaired by soil of this method to oil pollution, is remarkably improved soil
Repairing effect, petroleum hydrocarbon degradation rate is high, and energy conservation and environmental protection is easy to operate, strong operability, and processing cost is low, and application prospect is wide
It is wealthy.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
Present embodiment uses a kind of following technical scheme: repairing method of microorganism of oil-polluted soils, step
Suddenly are as follows:
(1) oil-polluted soils are collected into isolation reparation place to repair, while the contaminated soil of collection are dried and is pulverized,
To remove volatile hydrocarbon；
(2) complex micro organism fungicide is prepared: Phanerochaete chrysosporium, bacillus licheniformis, pseudomonas aeruginosa and xylose oxidation
After the completion of achromobacter culture, uniformly mixed according to the ratio of the 0.5-1:0.5-1:2-3:1-2 of bacterial strain concentration；
(3) composite bacteria agent, biomass castoff, surfactant are proportionally added separately to the soil in step (1), and
45-60 days sample detection repairing effects are reacted in uniformly mixing.
It is worth noting that, the mass concentration range of the pollution petroleum hydrocarbon of oil-polluted soils is in the step (1)
2%-12%。
In addition, in the step (3) biomass castoff using one of stalk, waste wood, peanut shell, withered grass or
A variety of, surfactant uses one of rhamnolipid, humic acid, Triton X-100, Tween80, surfactant
Additive amount is 0.5 ‰ -1.5 ‰.
Complex micro organism fungicide culture completion requires bacterial strain concentration to reach 10 in present embodiment step (2)9cfu/
ML, soil connect bacterium amount higher than 108cfu/kg；The activation culture based formulas of Phanerochaete chrysosporium are as follows: potato 20%, glucose
2%, agar 1.5%-2.0%, pH are natural；Fermentative medium formula are as follows: potato 0.2%, glucose 2.0%, KH2PO4 0.3%、
MgSO4·7H2O 0.15%、FeSO4·7H2O 0.1mg/L、CuSO4·5H2O 0.2mg/L, vitamin B1 8mg/L.Lichens bud
The activation medium of spore bacillus, pseudomonas aeruginosa and Achromobacter xylosoxidans forms: beef extract 0.5%, peptone 1%,
NaCl 0.5%, pH 7.0-7.2；Fermentation medium composition: hydrolysis sugar 2.5%, corn pulp 2.5%, KH2PO4 0.15%、MgSO4
0.04%, urea 0.4%, pH 6.8-7.0.
15-30 DEG C of reaction temperature of control in step (3), keeping moisture content in reaction system is 20%-30%, nitrogen: phosphorus matter
Amount adjusts pH6.0-8.0, keeps the bacterium of addition in 3-7 days, and periodically turn over ventilation, control the reaction prepared than being 8-12:1
System banks up into stack.
Present embodiment substantially increases the repairing effect of oil-polluted soils, makes rehabilitating soil that state can be fully achieved
Family's required standard is repaired efficiently, and high treating effect, petroleum hydrocarbon degradation rate is up to 80% or more, at low cost, not only economically feasible, and
Strong operability, is suitable for large scale processing oil-polluted soils, environmentally protective, do not generate secondary pollution, has wide city
Field application prospect.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (8)
1. a kind of repairing method of microorganism of oil-polluted soils, which is characterized in that the steps include:
(1) oil-polluted soils are collected into isolation reparation place to repair, while the contaminated soil of collection are dried and is pulverized,
To remove volatile hydrocarbon；
(2) complex micro organism fungicide is prepared: Phanerochaete chrysosporium, bacillus licheniformis, pseudomonas aeruginosa and xylose oxidation
After the completion of achromobacter culture, uniformly mixed according to the ratio of the 0.5-1:0.5-1:2-3:1-2 of bacterial strain concentration；
(3) composite bacteria agent, biomass castoff, surfactant are proportionally added separately to the soil in step (1), and
45-60 days sample detection repairing effects are reacted in uniformly mixing.
2. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that the step
Suddenly the mass concentration range of the pollution petroleum hydrocarbon of oil-polluted soils is 2%-12% in (1).
3. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that the step
Suddenly complex micro organism fungicide culture completion requires bacterial strain concentration to reach 10 in (2)9Cfu/mL, soil connect bacterium amount higher than 108cfu/
kg。
4. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that described
The activation culture based formulas of Phanerochaete chrysosporium in step (2) are as follows: potato 20%, glucose 2%, agar 1.5%-2.0%, pH
It is natural；Fermentative medium formula are as follows: potato 0.2%, glucose 2.0%, KH2PO4 0.3%、MgSO4·7H2O 0.15%、
FeSO4·7H2O 0.1mg/L、CuSO4·5H2O 0.2mg/L, vitamin B1 8mg/L.
5. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that described
The activation medium composition of bacillus licheniformis, pseudomonas aeruginosa and Achromobacter xylosoxidans in step (2): beef extract
0.5%, peptone 1%, NaCl 0.5%, pH 7.0-7.2；Fermentation medium composition: hydrolysis sugar 2.5%, corn pulp 2.5%,
KH2PO4 0.15%、MgSO40.04%, urea 0.4%, pH 6.8-7.0.
6. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that the step
Suddenly biomass castoff uses one of stalk, waste wood, peanut shell, withered grass or a variety of in (3).
7. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that described
Surfactant uses one of rhamnolipid, humic acid, Triton X-100, Tween80, surface-active in step (3)
The additive amount of agent is 0.5 ‰ -1.5 ‰.
8. a kind of repairing method of microorganism of oil-polluted soils according to claim 1, which is characterized in that the step
Suddenly 15-30 DEG C of reaction temperature of control in (3), keeping moisture content in reaction system is 20%-30%, and nitrogen: phosphorus mass ratio is 8-12:
1, adjust pH6.0-8.0, keep the bacterium of addition in 3-7 days, and periodically turn over ventilation, control the reaction system prepared bank up it is in heaps
Shape.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109454105A (en) * | 2019-01-15 | 2019-03-12 | 杭州造品科技有限公司 | A kind of restorative procedure of high concentration petroleum hydrocarbon contaminated soil |
| CN109590319A (en) * | 2018-12-10 | 2019-04-09 | 中国石油大学(华东) | Middle low concentration oil-containing soil contaminated with hydrocarbons biological humic green in-situ renovation technique |
| CN109679863A (en) * | 2018-12-12 | 2019-04-26 | 河南科技学院 | A kind of oil-polluted soils microorganism remediation reagent and preparation method thereof |
| CN110116132A (en) * | 2019-01-31 | 2019-08-13 | 永清环保股份有限公司 | A kind of method that charcoal base composite bacteria agent strengthens remedying oil-polluted soils |
| CN110802098A (en) * | 2019-09-25 | 2020-02-18 | 内蒙古久科康瑞环保科技有限公司 | Method for recycling oil-based rock debris |
| CN111533615A (en) * | 2020-04-02 | 2020-08-14 | 西安石油大学 | Composition for repairing high-oil-content clay and repairing method thereof |
| CN111872100A (en) * | 2020-07-14 | 2020-11-03 | 天津绿缘环保工程股份有限公司 | Method for restoring petroleum hydrocarbon contaminated soil |
| CN112642857A (en) * | 2020-11-18 | 2021-04-13 | 山东高科联合环保科学研究院有限公司 | Marine microorganism remediation method for petroleum-polluted soil |
| CN113714275A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer multistage inoculation microorganism regulation and control method |
| CN113714272A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer volatile organic compound multi-stage inoculation microorganism regulation and control method |
| CN113717904A (en) * | 2021-10-15 | 2021-11-30 | 黑龙江八一农垦大学 | Composite bacterium and microbial inoculum for shale oil pollution degradation, and preparation method and application thereof |
| CN113999786A (en) * | 2021-07-20 | 2022-02-01 | 苏州市宏宇环境科技股份有限公司 | Preparation method of immobilized soil remediation strain and application of immobilized soil remediation strain in biological heap soil remediation |
| CN114101318A (en) * | 2020-08-26 | 2022-03-01 | 中国石油天然气股份有限公司 | Treatment method of petroleum-polluted soil |
| CN114160568A (en) * | 2021-11-12 | 2022-03-11 | 焦致劲 | Novel petroleum hydrocarbon polluted soil biological treatment device and method |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109590319A (en) * | 2018-12-10 | 2019-04-09 | 中国石油大学(华东) | Middle low concentration oil-containing soil contaminated with hydrocarbons biological humic green in-situ renovation technique |
| CN109679863A (en) * | 2018-12-12 | 2019-04-26 | 河南科技学院 | A kind of oil-polluted soils microorganism remediation reagent and preparation method thereof |
| CN109679863B (en) * | 2018-12-12 | 2022-11-15 | 河南科技学院 | Microbial remediation reagent for petroleum-polluted soil and preparation method thereof |
| CN109454105A (en) * | 2019-01-15 | 2019-03-12 | 杭州造品科技有限公司 | A kind of restorative procedure of high concentration petroleum hydrocarbon contaminated soil |
| CN110116132A (en) * | 2019-01-31 | 2019-08-13 | 永清环保股份有限公司 | A kind of method that charcoal base composite bacteria agent strengthens remedying oil-polluted soils |
| CN110802098A (en) * | 2019-09-25 | 2020-02-18 | 内蒙古久科康瑞环保科技有限公司 | Method for recycling oil-based rock debris |
| CN111533615A (en) * | 2020-04-02 | 2020-08-14 | 西安石油大学 | Composition for repairing high-oil-content clay and repairing method thereof |
| CN113714275A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer multistage inoculation microorganism regulation and control method |
| CN113714272A (en) * | 2020-05-24 | 2021-11-30 | 中国环境科学研究院 | Soil layer volatile organic compound multi-stage inoculation microorganism regulation and control method |
| CN111872100A (en) * | 2020-07-14 | 2020-11-03 | 天津绿缘环保工程股份有限公司 | Method for restoring petroleum hydrocarbon contaminated soil |
| CN114101318A (en) * | 2020-08-26 | 2022-03-01 | 中国石油天然气股份有限公司 | Treatment method of petroleum-polluted soil |
| CN112642857A (en) * | 2020-11-18 | 2021-04-13 | 山东高科联合环保科学研究院有限公司 | Marine microorganism remediation method for petroleum-polluted soil |
| CN113999786A (en) * | 2021-07-20 | 2022-02-01 | 苏州市宏宇环境科技股份有限公司 | Preparation method of immobilized soil remediation strain and application of immobilized soil remediation strain in biological heap soil remediation |
| CN113717904A (en) * | 2021-10-15 | 2021-11-30 | 黑龙江八一农垦大学 | Composite bacterium and microbial inoculum for shale oil pollution degradation, and preparation method and application thereof |
| CN114160568A (en) * | 2021-11-12 | 2022-03-11 | 焦致劲 | Novel petroleum hydrocarbon polluted soil biological treatment device and method |
| CN114160568B (en) * | 2021-11-12 | 2022-11-01 | 焦致劲 | Petroleum hydrocarbon contaminated soil biological treatment device and method |
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Application publication date: 20181207 |