CN110802098A - Method for recycling oil-based rock debris - Google Patents
Method for recycling oil-based rock debris Download PDFInfo
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- CN110802098A CN110802098A CN201910911966.3A CN201910911966A CN110802098A CN 110802098 A CN110802098 A CN 110802098A CN 201910911966 A CN201910911966 A CN 201910911966A CN 110802098 A CN110802098 A CN 110802098A
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- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
Abstract
The invention discloses a method for recycling oil-based rock debris, which comprises the following steps: pretreating the initial oil-based rock debris and the straws to obtain oil-based rock debris and crushed straws; culturing petroleum degrading bacteria by using a liquid culture medium to obtain petroleum degrading bacteria liquid; stacking oil-based rock debris, crushed straws and excrement together in a layering manner to obtain an interlayer stack body, wherein the interlayer stack body sequentially comprises a crushed straw layer, an oil-based rock debris layer, a crushed straw layer, an excrement layer and a crushed straw layer from bottom to top; putting the petroleum degrading bacteria liquid on the uppermost crushed straw layer; and performing heat preservation treatment on the interlayer stack after the petroleum degrading bacteria liquid is put in, and preserving heat until the oil content of the oil-based rock debris layer is less than or equal to the preset oil content. According to the method, the interlayer stack of the crushed straw layer, the oil-based rock debris layer, the crushed straw layer, the excrement layer and the crushed straw layer is constructed, and the oil-based rock debris is effectively decomposed by using the petroleum degrading bacterial liquid, so that the oil-based rock debris is recycled.
Description
Technical Field
The invention relates to the technical field of solid waste treatment, in particular to a method for recycling oil-based rock debris.
Background
Natural gas is also buried in underground closed geological structures like crude oil, and some are stored at the same level as crude oil and some exist independently. For natural gas that is stored at the same level as the crude oil, it is produced along with the crude oil.
The shale gas refers to unconventional natural gas which is endowed in a reservoir rock system mainly containing organic-rich shale, is biochemical formation gas, thermal formation gas or a mixture of the biochemical formation gas and the thermal formation gas which are continuously generated, can exist in a free state in natural cracks and pores, exists on the surfaces of kerogen and clay particles in an adsorption state, and is stored in a very small amount in the kerogen and the asphaltene in a dissolved state, wherein the proportion of the free gas is generally 20-85%.
The oil-based drilling fluid has the advantages of high temperature resistance, salt and calcium corrosion resistance, contribution to well wall stability, good lubricating property, small damage to an oil-gas layer and the like. Therefore, with the increasing development of unconventional energy sources such as shale gas, dense gas and the like in the world, the oil-based drilling fluid technology has become one of the key technologies for drilling shale gas horizontal wells. Oil-based cuttings are "waste" generated when oil-based drilling fluids are injected to open the "path" for production of shale gas. Because the oil-based rock debris contains mineral oil, phenolic compounds, heavy metals and other toxic substances, the direct discharge of the oil-based rock debris can cause pollution to soil, earth surfaces and underground water, and directly or indirectly cause harm to the health of plants, animals and human beings, and the oil-based rock debris is listed as national hazardous waste (national hazardous waste list, HW08), and can cause serious harm to the surrounding ecological environment if the oil-based rock debris is directly discharged without being treated. Therefore, it must be treated before it can be discharged. At present, the common treatment modes of the oil-based rock debris comprise a solvent extraction technology, a high-temperature cracking technology, an incineration technology, a physical and chemical separation technology, a solidification technology and the like. In a common oil-based rock debris treatment mode, secondary pollution is easily caused in the treatment process, only the treatment of harmful substances in the oil-based rock debris is generally considered, and the recovery and utilization of resources are not realized, so that the resource waste is caused to a certain extent.
Disclosure of Invention
Therefore, a method for recycling oil-based rock debris is needed to be provided for solving the problems that in the prior art, secondary pollution is easily caused in the treatment process of the oil-based rock debris, and the recycling and utilization of resources cannot be realized.
A method of resource treatment of oil-based cuttings, comprising:
performing deoiling and dewatering pretreatment on the initial oil-based rock debris to obtain oil-based rock debris;
drying and crushing the straws to obtain crushed straws;
culturing petroleum degrading bacteria by using a liquid culture medium to obtain petroleum degrading bacteria liquid;
stacking the oil-based rock debris, the crushed straws and the excrement in a layering manner to obtain an interlayer stack, wherein the interlayer stack sequentially comprises a crushed straw layer, an oil-based rock debris layer, a crushed straw layer, an excrement layer and a crushed straw layer from bottom to top;
putting the petroleum degrading bacteria liquid on the uppermost crushed straw layer; and
and carrying out heat preservation treatment on the interlayer stack body after the petroleum degrading bacterial liquid is put in the interlayer stack body until the oil content of the interlayer stack body is lower than the preset oil content.
In one embodiment, the preset oil content is 0.03%
In one embodiment, the step of performing a deoiling and dewatering pretreatment on the initial oil-based cuttings comprises:
performing an oil content test on said initial oil-based cuttings, an
Adopting different pretreatment modes for the initial oil-based rock debris according to different oil contents, reducing the oil content of the initial oil-based rock debris to be less than or equal to 10%, and reducing the water content to be less than or equal to 40%, so as to obtain the oil-based rock debris;
when the oil content is more than or equal to 15%, the pretreatment mode is that the initial oil-based rock debris and water are mixed and stirred uniformly, and then centrifugal deoiling and dewatering treatment is carried out; and when the oil content is less than 15%, directly performing centrifugal dehydration and deoiling treatment on the initial oil-based rock debris in the pretreatment mode.
In one embodiment, the drying and crushing treatment of the straws comprises the following steps:
drying the straw to a moisture content of less than 5%, and
and crushing the straws into short structures with the lengths of 3cm-8cm to obtain the crushed straws.
In one embodiment, the colony number of the petroleum degrading bacteria in the petroleum degrading bacteria liquid is 109cfu/mL-1010cfu/mL。
In one embodiment, the petroleum-degrading bacteria are selected from Bacillus subtilis, Pseudomonas stutzeri, or a mixture of Bacillus subtilis and Pseudomonas stutzeri.
In one embodiment, the culturing the petroleum-degrading bacteria with a liquid medium to obtain a petroleum-degrading bacteria solution comprises:
inoculating Bacillus subtilis and Pseudomonas stutzeri to culture medium at colony number ratio of 1:2, and culturing until total number of colonies reaches 109cfu/mL-1010And obtaining the petroleum degrading bacterial liquid by cfu/mL.
In one embodiment, the thickness of each straw layer is 10cm-15cm, the thickness of the oil-based rock debris layer is 10cm-15cm, and the thickness of the excrement layer is 20cm-25 cm.
In one embodiment, the petroleum-degrading bacteria liquid is put on the uppermost crushed straw layer, wherein the petroleum-degrading bacteria liquid is put inThe amount is 0.5L/m2-1.5L/m2。
In one embodiment, the heat preservation treatment of the interlayer stack after the bacterial liquid is put into the interlayer stack includes: covering the interlayer stack body with a transparent plastic film, and keeping the temperature at 30-40 ℃.
The method for recycling the oil-based rock debris is provided according to the characteristics of the oil-based rock debris, constructs an interlayer stack of a straw layer, the oil-based rock debris layer, the straw layer, an excrement layer and the straw layer, and effectively decomposes organic pollutants in the oil-based rock debris by using petroleum degrading bacteria liquid, so that the oil content of the oil-based rock debris is reduced. The method carries out deep treatment on the oil-based rock debris produced in the process of mining unconventional oil and gas resources such as shale gas, dense gas and the like, enhances the degradation and removal of biological toxic substances and organic components in the rock debris, and ensures that the oil content of the oil-based rock debris treated by the interlayer stack can reach the use standard lower than the standard specified in the standard for controlling pollutants in agricultural sludge, so that the oil-based rock debris can be returned to the field as fertilizer, thereby realizing the resource treatment of the oil-based rock debris.
Drawings
FIG. 1 is a schematic cross-sectional view of a sandwich stack in an embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the method for recycling oil-based rock debris according to the present invention is further described in detail by the following embodiments with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the present invention, wherein rpm and r.min-1Expressed in revolutions per minute (rpm), cfu/mL in total number of bacterial colonies contained per mL of sample, cm in centimetres, L/m2Expressed in liters per square meter, t in tons of mass, min in minutes of time, and d in days of time. In the invention, the water content of the oil-based rock debris and the straws is detected according to the method of GB 7172-1987 soil moisture determination StandardAnd (4) percent by mass.
The embodiment of the invention provides a method for recycling oil-based rock debris, which comprises the following steps:
performing deoiling and dewatering pretreatment on the initial oil-based rock debris to obtain oil-based rock debris;
drying and crushing the straws to obtain crushed straws;
culturing petroleum degrading bacteria by using a liquid culture medium to obtain petroleum degrading bacteria liquid;
stacking the oil-based rock debris, the crushed straws and the excrement in a layering manner to obtain an interlayer stack, wherein the interlayer stack sequentially comprises a crushed straw layer, an oil-based rock debris layer, a crushed straw layer, an excrement layer and a crushed straw layer from bottom to top;
putting the petroleum degrading bacteria liquid on the uppermost crushed straw layer; and
and carrying out heat preservation treatment on the interlayer stack after the bacterial liquid is put in the interlayer stack until the oil content of the interlayer stack is less than or equal to the preset oil content.
According to the method for recycling the oil-based rock debris, disclosed by the embodiment of the invention, the interlayer stack of the straw layer, the oil-based rock debris layer, the straw layer, the excrement layer and the straw layer is constructed, and the oil-degrading bacteria liquid is utilized to effectively decompose the organic pollutants in the oil-based rock debris, so that the oil content of the oil-based rock debris is reduced. The straw and the excrement which are used as raw materials of the interlayer stack body can ensure a carbon source, a nitrogen source and a carbon-nitrogen ratio required by the growth of petroleum degrading bacteria, and the straw has a certain surface area and pores, so that the circulation of oxygen can be better increased, and the degradation of organic matters in the oil-based rock debris can be accelerated. The final oil content of the oil-based rock debris is different under different application conditions, and the preset oil content of the interlayer stack body which is required to be less than or equal to the oil content is set according to different application conditions.
In a preferred embodiment, the predetermined debris has an oil content of 0.03%. The invention relates to a method for constructing an interlayer stack body by using straw, oil-based rock debris and excrement which are difficult to treat, and wastes are made by wastes, wherein when the preset oil content is 0.03%, the interlayer stack body comprising the degraded oil-based rock debris can be used as cultivation soil after being fully stirred and mixed, so that the method is an environment-friendly technology for eliminating pollution of the oil-based rock debris and generating no secondary pollution.
In one embodiment, the method for resource treatment of the oil-based rock debris comprises the following steps of: detecting the oil content of the initial oil-based rock debris, and adopting different pretreatment modes for the initial oil-based rock debris according to different oil contents to reduce the oil content of the initial oil-based rock debris to be less than or equal to 10% and reduce the water content to be less than or equal to 40% so as to obtain the oil-based rock debris; when the oil content is more than or equal to 15%, the pretreatment mode is that the initial oil-based rock debris and water are mixed and stirred uniformly, and then centrifugal deoiling and dewatering treatment is carried out; when the oil content is 15% lower, the pretreatment mode is to directly carry out centrifugal dehydration and deoiling treatment on the initial oil-based rock debris. Preferably, in the step of performing the deoiling and dewatering pretreatment on the initial oil-based rock debris, the centrifugal deoiling and dewatering may perform the deoiling and dewatering treatment on the oil-based rock debris by using a centrifuge or a plate and frame filter press. Preferably, when the centrifuge is used for deoiling and dewatering the oil-based rock debris, the rotation speed of the centrifuge is 900 rpm. Before the initial oil-based rock debris is deoiled and dehydrated, the oil content is detected, and an obstructed pretreatment mode is performed according to a detection result, so that the pretreatment has better pertinence and a better treatment effect. The oil-based rock debris with the oil content higher than 15% is difficult to be reduced to below 10% by direct centrifugal deoiling and dewatering, so that the oil content of the initial oil-based rock debris can be reduced to below 10% and the water content can be reduced to about 40% by performing deoiling and dewatering treatment after water is added for stirring. Preferably, when the initial oil-based rock debris with the oil content higher than 15% is pretreated, the volume ratio of the added water to the initial oil-based rock debris is 1.5: 1-3: 1, and preferably, the volume ratio of the added water to the initial oil-based rock debris is 2: 1. The oil-containing rate of the oil-based rock debris can be preliminarily reduced by pretreating the oil-based rock debris, so that the degradation difficulty of subsequent petroleum degrading bacteria is reduced, meanwhile, the oil can be recycled, and the resource utilization of the oil-based rock debris is further realized.
In one embodiment, the manure is poultry manure. Further, before stacking the interlayer stack, the poultry manure is ventilated and dried, and the water content of the poultry manure is reduced to about 10% for later use.
In one embodiment, the drying and crushing treatment of the straw comprises: drying the straws until the water content is less than 5%, and crushing the straws into short structures with the lengths of 3cm-8cm to obtain the crushed straws. Preferably, the straws are crushed into a short structure with the length of 5 cm. The crushed straws are obtained by drying and crushing the straws, and the crushed straws have better surface area and pores, so that the circulation of oxygen in the interlayer stack body can be better increased, and the degradation of organic matters in the oil-based rock debris can be accelerated.
Preferably, the straw can be corn stalk, rice straw, bean stalk or peanut skin and other crop wastes.
In one embodiment, the number of colonies of the petroleum-degrading bacteria in the petroleum-degrading bacteria solution is 109cfu/mL-1010cfu/mL. The concentration of the petroleum degrading bacteria in the petroleum degrading bacteria liquid is limited, so that the degradation effect is ensured, and the waste of the culture medium caused by overhigh concentration of bacteria in the bacteria liquid is avoided.
In one embodiment, the petroleum-degrading bacteria are selected from bacillus subtilis, pseudomonas stutzeri, or a mixture of bacillus subtilis and pseudomonas stutzeri. The petroleum degrading bacteria can select strains known in the field and having good degradation effect on petroleum organic matters, and the patent application with the publication number of CN105087538A discloses a petroleum degrading microbial inoculum and a preparation method and application thereof, wherein the mixed bacterial solution of Chryseobacterium, achromobacter and Burkholderia is disclosed; the patent application with publication number CN104017747A discloses a petroleum degrading bacterium in oil-containing sludge and application thereof, wherein corynebacterium glutamicum is disclosed; the above petroleum-degrading bacteria are all applicable to the present application, and the contents of both applications are hereby incorporated in their entirety into the present application.
In a preferred embodiment, theThe method for culturing the petroleum degrading bacteria by using the liquid culture medium to obtain the petroleum degrading bacteria liquid comprises the following steps: inoculating Bacillus subtilis and Pseudomonas stutzeri to culture medium at colony number ratio of 1:2, and culturing until total number of colonies reaches 109cfu/mL-1010cfu/mL to obtain the petroleum degrading bacterial liquid. In this example, a mixture of Bacillus subtilis and Pseudomonas stutzeri was used, and the number of petroleum-degraded colonies obtained was 109cfu/mL-1010cfu/mL, the obtained petroleum degrading bacterial liquid can effectively degrade organic matters in the oil-based rock debris.
In one embodiment, each layer of the crushed straw layer has a thickness of 10cm to 15cm, the layer of the oil-based cuttings has a thickness of 10cm to 15cm, and the layer of the excrement has a thickness of 20cm to 25 cm.
In the embodiment of the invention, the carbon-nitrogen ratio in the interlayer stack is suitable for culturing petroleum degrading bacteria by controlling the proportion of the oil-based rock debris, the crushed straw and the excrement, so that the degradation of organic matters in the oil-based rock debris is promoted.
In one embodiment, the petroleum-degrading bacteria liquid is put on the uppermost crushed straw layer, wherein the putting amount of the petroleum-degrading bacteria liquid is 0.5L/m2-1.5L/m2. Preferably, the adding amount of the petroleum degrading bacteria liquid is 1L/m2. In the bacterial liquid input volume within range in this embodiment, can guarantee the abundant quick degradation of organic matter in the oil base detritus, can not waste the bacterial liquid simultaneously.
In one embodiment, the heat preservation treatment of the sandwich stack after the bacterial liquid is put into the sandwich stack includes: and covering the interlayer stack body by adopting a transparent plastic film, wherein the interlayer stack body is 30-40 ℃. Preferably, the temperature of the sandwich stack is maintained at 35 ℃. The heat preservation mode in the embodiment is simple to operate and low in cost.
Example 1
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 14.7%, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 9.36% and the water content to 35%, and weighing a certain amount of oil-based rock debris for later use. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 5cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at colony ratio of 1:2 into 20L glucose culture medium, and standing at 25 deg.C and 160r min-1The constant temperature shaking table is used for about 16 hours, and the number of viable bacteria colonies in the bacterial liquid reaches 1010cfu/mL is reserved.
(4) Stacking straw-oil-based rock debris-straw-poultry excrement-straw sandwich stacks, wherein ① is flat and 10m 2②, uniformly paving 10 cm-thick crushed straw layers on the bottommost layer of the prepared crushed straws, ③ uniformly paving 10 cm-thick oil-based rock debris layers on the paved crushed straw layers by using prepared oil-based rock debris, ④ uniformly paving 10 cm-thick crushed straw layers on the oil-based rock debris layers, ⑤ paving 20 cm-thick poultry excrement layers on the crushed straw layers paved in step ④, ⑥ finally covering 10 cm-thick straws on the poultry excrement layers, ⑦ uniformly spraying 1L of prepared petroleum degrading bacteria liquid on the uppermost layer of the straw layers, ⑧ covering the whole interlayer straw pile by using a transparent plastic film, and culturing for about 60 days while keeping the temperature at about 35 ℃.
Example 2
(1) Preparing oil-based rock debris and poultry excrement: detecting the oil content of the initial oil-based rock debris, wherein the oil content is 18.9%, adding water with the volume twice that of the initial oil-based rock debris, uniformly mixing and stirring, performing centrifugal deoiling and dewatering treatment, reducing the oil content of the pretreated oil-based rock debris to 9.89%, and weighing a certain amount of oil-based rock debris for later use, wherein the water content of the pretreated oil-based rock debris is 35%. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 5cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating bacillus subtilis and pseudomonas stutzeri to 20L of glucose culture medium according to the colony number ratio of 1:2, and culturing in a constant-temperature shaking table at 25 ℃ and 160r min < -1 > for about 16h, wherein the colony number of viable bacteria in the bacterial liquid reaches 1010cfu/mL for later use.
(4) Stacking straw-oil-based rock debris-straw-poultry excrement-straw sandwich stacks, wherein ① is flat and 10m 2② spreading the crushed straws uniformly on the bottom layer with a thickness of 10cm, ③ spreading the oil-based detritus layer uniformly on the top layer with a thickness of 10cm, ④ spreading the crushed straw layer uniformly on the oil-based detritus layer with a thickness of 10cm, ⑤ spreading the crushed straws layer laid in step ④ with 20cThe method comprises the steps of preparing a poultry excrement layer with the thickness of m, ⑥ covering straws with the thickness of 10cm on the poultry excrement layer, ⑦ uniformly spraying the prepared petroleum degrading bacteria liquid on the uppermost straw layer according to the amount of 1L per square meter, ⑧ covering the whole interlayer stack by using a transparent plastic film, keeping the temperature at about 35 ℃ and culturing for about 60 days.
Example 2 is substantially the same as example 1 except that the oil content of the initial oil-based rock debris is different, so that different pretreatment methods are adopted for the initial oil-based rock debris, and the oil content and the water content of the pretreated oil-based rock debris are different in this example.
Example 3
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 18.9%, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 13.79% and the water content to 35%, and weighing a certain amount of oil-based rock debris for later use. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 5cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at colony ratio of 1:2 into 20L glucose culture medium, and culturing in constant temperature shaking table at 25 deg.C and 160r min-1 for about 16hThe number of viable bacteria in the liquid reaches 1010cfu/mL is reserved.
(4) Stacking straw-oil-based rock debris-straw-poultry excrement-straw sandwich stacks, wherein ① is flat and 10m 2②, uniformly paving 10 cm-thick crushed straw layers on the bottommost layer of the prepared crushed straws, ③ uniformly paving 10 cm-thick oil-based rock debris layers on the paved crushed straw layers by using prepared oil-based rock debris, ④ uniformly paving 10 cm-thick crushed straw layers on the oil-based rock debris layers, ⑤ paving 20 cm-thick poultry excrement layers on the crushed straw layers paved in step ④, ⑥ finally covering 10 cm-thick straws on the poultry excrement layers, ⑦ uniformly spraying 1L of prepared petroleum degrading bacteria liquid on the uppermost layer of the straw layers, ⑧ covering the whole interlayer straw pile by using a transparent plastic film, and culturing for about 60 days while keeping the temperature at about 35 ℃.
Example 3 is substantially the same as example 1 except that the oil content of the oil-based cuttings after pretreatment is different.
Example 3 the same initial oil-based rock debris was used as in example 2, and when the oil content of the initial oil-based rock debris was greater than 15%, the oil-based rock debris was directly dried by a rock debris dryer, and the oil content was reduced to about 13%, the oil content did not change significantly even after the pretreatment time was extended, thus indicating that the oil content of the initial oil-based rock debris having an oil content of greater than 15% was reduced to less than 10%, and the pretreatment method of example 2 was used to perform the pretreatment.
Example 4
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 14.7%, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 9.36%, and the water content is 35%, and weighing a certain amount for later use. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 1cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at a ratio of 1:2 to 20L glucose culture medium, and culturing in constant temperature shaking table at 25 deg.C and 160r min-1 for about 16h to obtain bacterial liquid containing viable bacteria at colony count of 1010cfu/mL is reserved.
(4) Stacking straw-oil-based rock debris-straw-poultry excrement-straw sandwich stacks, wherein ① is flat and 10m 2②, uniformly paving 10 cm-thick crushed straw layers on the bottommost layer of the prepared crushed straws, ③ uniformly paving 10 cm-thick oil-based rock debris layers on the paved crushed straw layers by using prepared oil-based rock debris, ④ uniformly paving 10 cm-thick crushed straw layers on the oil-based rock debris layers, ⑤ paving 20 cm-thick poultry excrement layers on the crushed straw layers paved in step ④, ⑥ finally covering 10 cm-thick straws on the poultry excrement layers, ⑦ uniformly spraying 1L of prepared petroleum degrading bacteria liquid on the uppermost layer of the straw layers, ⑧ covering the whole interlayer straw pile by using a transparent plastic film, and culturing for about 60 days while keeping the temperature at about 35 ℃.
Example 4 is substantially the same as example 1 except that in example 4, (2) a step of preparation of rice straw, in which the length of the pulverized straw is about 1cm, is used.
Example 5
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 14.7%, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 9.36%, and the water content is 35%, and weighing a certain amount for later use. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 5cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at a ratio of 1:2 to 20L glucose culture medium, and culturing in constant temperature shaking table at 25 deg.C and 160r min-1 for about 16h to obtain bacterial liquid containing viable bacteria at colony count of 1010cfu/mL is reserved.
(4) Stacking straw-oil-based rock debris-straw-poultry excrement-straw sandwich stacks, wherein ① is flat and 10m 2② spreading crushed straw layer of 5cm thickness on the bottom layer, ③ spreading oil-based rock debris layer of 10cm thickness on the bottom layer, ④ spreading crushed straw layer of 5cm thickness on the oil-based rock debris layer, ⑤ spreading poultry feces layer of 5cm thickness on the crushed straw layer spread in step ④, ⑥ covering straw of 5cm thickness on the poultry feces layer, ⑦ spraying petroleum degrading bacteria liquid to the top straw layer in 0.5L/m, ⑧ spraying transparent plastic filmThe whole sandwich stack is covered, the temperature is kept at about 35 ℃, and the culture lasts for about 60 days.
Example 5 is substantially the same as example 1 except that the thickness of each layer of the sandwich layer stack is different and the amount of the petroleum-degrading bacteria is different in (4) the step of stacking the sandwich layer stack of straw-oil-based rock debris-straw-poultry litter-straw. In this example, (4) in the step of stacking the straw-oil-based rock debris-straw-poultry excrement-straw sandwich stack, the thickness of each layer of the pulverized straw layer is 5cm, the thickness of the oil-based rock debris layer is 5cm, and the thickness of the poultry excrement layer is 10 cm; the petroleum degrading bacteria liquid is uniformly sprayed on the uppermost straw layer according to the amount of 0.5L per square meter.
In examples 1 to 5, each of which constructed a sandwich stack, the schematic cross-sectional view of which is shown in fig. 1, the sandwich stack comprises, in order from bottom to top: the first straw layer 1, the oil-based detritus layer 2, the second straw layer 3, the poultry excrement layer 4 and the third straw layer 5. Wherein, the first straw layer 1, the oil-based detritus layer 2, the second straw layer 3, the poultry excrement layer 4 and the third straw layer 5.
Comparative example 1
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 14.7 percent and is lower than 15 percent, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 9.36 percent and the water content to 35 percent, and weighing a certain amount of oil-based rock debris for later use. Drying the feces in the sun, air drying, and weighing a certain amount for use
(2) According to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at colony ratio of 1:2 into 10L glucose culture medium, and standing at 25 deg.C and 160r min-1The constant temperature shaking table is used for about 16 hours, and the number of viable bacteria colonies in the bacterial liquid reaches 1010cfu/mL is reserved.
(3) 1m is3Oil-based rock debris, 2m3Mixing livestock and fowl feces with 10L bacteria liquid, stirring, and stacking the mixture at 10m2The mixed stack was covered with a transparent plastic film, and the temperature was maintained at about 35 ℃ for about 60 days.
Example 1 is different from comparative example 1 in that a composting method of a straw-oil-based rock debris-straw-poultry manure-straw interlayer stack and then spraying a bacterial liquid to the uppermost straw layer is adopted in example 1; in the comparative example 1, the oil-based rock debris, the livestock and poultry manure and the bacteria liquid are mixed uniformly and stacked.
Comparative example 2
(1) Preparing oil-based rock debris and poultry excrement: and (3) detecting the oil content of the initial oil-based rock debris, wherein the oil content is 14.7 percent and is lower than 15 percent, drying the oil-based rock debris by using a rock debris drying machine, reducing the oil content of the oil-based rock debris obtained after pretreatment to 9.36 percent and the water content to 35 percent, and weighing a certain amount of oil-based rock debris for later use. Drying the feces of the poultry in the sun, ventilating and drying, and weighing a certain amount for later use.
(2) Preparing corn straws: drying the corn straws at a ventilation position, reducing the water content to 10 percent, crushing the corn straws into short pieces with the length of about 5cm by a crusher, and taking a certain amount of the corn straws for later use.
(3) Preparing a bacterial liquid:
according to the peptone: beef extract: NaCl: distilled water: agar-1: 0.5: 0.5: 100: preparing beef extract peptone culture medium according to the mass ratio of 1.5, adjusting the pH value to 7.2, and sterilizing at 121 ℃ for 20 min.
According to the basic inorganic salt solution: yeast cream: 20% glucose solution ═ 100: 0.1: 5, preparing 20L of glucose culture medium, and sterilizing at 121 ℃ for 20 min.
Respectively coating bacillus subtilis and pseudomonas stutzeri on beef extract peptone culture medium, and culturing at constant temperature of 37 ℃ for 2 days; selecting a single colony, and repeatedly performing streak culture until pure strains of bacillus subtilis and pseudomonas stutzeri are obtained;
inoculating Bacillus subtilis and Pseudomonas stutzeri at colony ratio of 1:2 into 10L glucose culture medium, and standing at 25 deg.C and 160r min-1The constant temperature shaking table is used for about 16 hours, and the number of viable bacteria colonies in the bacterial liquid reaches 1010cfu/mL is reserved.
(4) 1m is3Oil-based rock debris, 2m3Livestock and poultry manure of 3m3Mixing the crushed straw with 10L of bacteria liquid, fully stirring and mixing, and stacking the mixed mixture in a 10m pile2The mixed stack was covered with a transparent plastic film, and the temperature was maintained at about 35 ℃ for about 60 days.
The comparative example 2 is different from the example 1 in that a composting mode that a straw-oil-based rock debris-straw-poultry manure-straw interlayer stack is adopted in the example 1, and then a bacterial liquid is sprayed on the uppermost straw layer; in the comparative example 1, the oil-based rock debris, the livestock and poultry manure, the crushed straw and the bacteria liquid are mixed uniformly and stacked.
The oil-based cuttings and heaps of examples 1-4 and comparative examples 1-2 were tested for oil holdup, which in the present invention refers to the mass percent of oil in the sample to be tested, at different time points according to the method in the oil field oil-containing sludge comprehensive utilization pollution control Standard (DB 23/T1413-2010). Specific test results are shown in table 1, wherein 0d represents the oil content of the oil-based rock debris, and 0d represents the oil content of the mixture obtained by converting the oil-based rock debris into the piles in each of the examples and comparative examples; the oil contents of the bulk mixtures were determined at 15d, 30d and 60 d.
TABLE 1
As can be seen from table 1, in example 1, the oil content of the oil-based cuttings decreased to 0.07% after 30 days of culture; while comparative example 1 had an oil content of 1.08% at 60 days of culture and comparative example 2 had an oil content of 0.86% at 60 days of culture. Therefore, it can be seen that the method for recycling the oil-based rock debris in example 1 is shorter and has a higher degree of degradation of the organic substances in the oil-based rock debris than in comparative examples 1 and 2. The straw and the livestock and poultry manure in the embodiment 1 can ensure the carbon source, the nitrogen source and the carbon-nitrogen ratio required by the growth of bacteria, and the straw layer has sufficient surface area and pores, so that the circulation of oxygen in the interlayer stack can be better increased, and the degradation of organic matters in the oil-based rock debris can be accelerated. Due to the arrangement of the three straw layers, the obtained interlayer stack has good air permeability, so that petroleum degrading bacteria can be bred better, and the degradation of organic matters in the oil-based rock debris is promoted.
As can be seen from Table 1, according to the comparison of example 1 with examples 2 to 5, the oil content of the oil-based rock debris used for making the pack, the length of the pulverized straw, and the thickness of the interlayer all have important effects on the degradation rate of the organic matter and the time for the pack degradation. From the comparison between example 1 and example 3, it is understood that when the oil content of the oil-based cuttings is higher than 10%, the degradation takes longer, thereby extending the treatment period. From the comparison between example 1 and example 4, it is known that the length of the pulverized straw is too short, so that the air permeability of the pulverized straw layer is deteriorated, and the degradation time is also prolonged. As can be seen from the comparison between the embodiment 1 and the embodiment 5, the thickness of the feces is too low, the carbon nitrogen ratio in the corresponding stack is also relatively small, and the air permeability of the stack is poor when the thickness of the straw is too small, so that the growth of microorganisms is influenced, and the treatment period is prolonged.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A method for resource treatment of oil-based rock debris is characterized by comprising the following steps:
performing deoiling and dewatering pretreatment on the initial oil-based rock debris to obtain oil-based rock debris;
drying and crushing the straws to obtain crushed straws;
culturing petroleum degrading bacteria by using a liquid culture medium to obtain petroleum degrading bacteria liquid;
stacking the oil-based rock debris, the crushed straws and the excrement in a layering manner to obtain an interlayer stack, wherein the interlayer stack sequentially comprises a crushed straw layer, an oil-based rock debris layer, a crushed straw layer, an excrement layer and a crushed straw layer from bottom to top;
putting the petroleum degrading bacteria liquid on the uppermost crushed straw layer; and
and carrying out heat preservation treatment on the interlayer stack body after the petroleum degrading bacterial liquid is put in the interlayer stack body until the oil content of the interlayer stack body is less than or equal to the preset oil content.
2. The method of claim 1, wherein the predetermined oil cut is 0.03%.
3. The method of claim 1, wherein the step of subjecting the initial oil-based cuttings to a deoiling and dewatering pretreatment comprises:
performing an oil content test on said initial oil-based cuttings, an
Adopting different pretreatment modes for the initial oil-based rock debris according to different oil contents, reducing the oil content of the initial oil-based rock debris to be less than or equal to 10%, and reducing the water content to be less than or equal to 40%, so as to obtain the oil-based rock debris;
when the oil content is more than or equal to 15%, the pretreatment mode is that the initial oil-based rock debris and water are mixed and stirred uniformly, and then centrifugal deoiling and dewatering treatment is carried out; when the oil content is 15% lower, the pretreatment mode is to directly carry out centrifugal dehydration and deoiling treatment on the initial oil-based rock debris.
4. The method of claim 1, wherein the drying and pulverizing the straw comprises:
drying the straw to a moisture content of less than 5%, and
and crushing the straws into short structures with the lengths of 3cm-8cm to obtain the crushed straws.
5. The method according to claim 1, wherein the number of colonies of the petroleum-degrading bacteria in the petroleum-degrading bacteria solution is 109cfu/mL-1010cfu/mL。
6. The method according to claim 1, wherein the petroleum-degrading bacteria are selected from the group consisting of Bacillus subtilis, Pseudomonas stutzeri, and a mixture of Bacillus subtilis and Pseudomonas stutzeri.
7. The method of claim 1, wherein the step of culturing the petroleum-degrading bacteria in a liquid medium to obtain a petroleum-degrading bacteria solution comprises:
inoculating Bacillus subtilis and Pseudomonas stutzeri to culture medium at colony number ratio of 1:2, and culturing until total number of colonies reaches 109cfu/mL-1010cfu/mL to obtain the petroleum degrading bacterial liquid.
8. The method of any one of claims 1-7, wherein each of said comminuted straw layer has a thickness of 10cm to 15cm, said oil-based detritus layer has a thickness of 10cm to 15cm, and said fecal layer has a thickness of 20cm to 25 cm.
9. The method of claim 1, wherein the petroleum-degrading bacteria liquid is thrown into the uppermost crushed straw layerWherein the adding amount of the petroleum degrading bacteria liquid is 0.5L/m2-1.5L/m2。
10. The method according to claim 1, wherein the heat preservation treatment of the sandwich stack with the bacterial liquid put therein comprises:
and covering the interlayer stack body by adopting a transparent plastic film, wherein the temperature of the interlayer stack body is 30-40 ℃.
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