CN106190891A - Based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment soil with serious petroleum pollution or the method for greasy filth - Google Patents
Based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment soil with serious petroleum pollution or the method for greasy filth Download PDFInfo
- Publication number
- CN106190891A CN106190891A CN201610541245.4A CN201610541245A CN106190891A CN 106190891 A CN106190891 A CN 106190891A CN 201610541245 A CN201610541245 A CN 201610541245A CN 106190891 A CN106190891 A CN 106190891A
- Authority
- CN
- China
- Prior art keywords
- soil
- greasy filth
- complex
- sodium peroxydisulfate
- oxidation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/10—Nature of the water, waste water, sewage or sludge to be treated from quarries or from mining activities
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
- C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Water Supply & Treatment (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment soil with serious petroleum pollution or the method for greasy filth, oxidation processes is carried out as oxidant and surfactant with the use of to contaminated soil or greasy filth first with sodium peroxydisulfate complex, the most double access bacillus cereus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.Y0997) or the mixed bacterial of Phanerochaete chrysosporium (Phanerochaete sp.F0996), combined by the strengthening of chemical oxidation and fungus bacteria flora biological sequence and carry out soil or greasy filth strengthening Combined Treatment, it is remarkably improved the degradation efficiency of soil with serious petroleum pollution or greasy filth, and it is substantially shorter repairing efficiency.
Description
Technical field
The invention belongs to soil with serious petroleum pollution or the chemistry of greasy filth and technical field of biological remediation, be specifically related to oil
Field mining area utilizes chemical oxidation and fungus-bacteria flora biological reinforced strengthening Combined Treatment soil with serious petroleum pollution or greasy filth
Method.
Technical background
At present, along with the development of domestic and international petroleum industry, oil-polluted soils scope the most constantly expands.For petroleum wastewater
Dye soil processes so that it is reaches the effect recovering to utilize in the short period of time, is in oil-polluted soils governance process
Problem demanding prompt solution.For the process of pollutant, select which method optimum, except to consider processing pollutant location
Point, amount of pollutant number, outside the technical factor such as the length of the quality for the treatment of effect, the length of required time and process, process
The height of expense is a highly important factor.
In recent years, bioremediation technology is at home and abroad obtained for and develops faster, and a collection of have special physiological biochemistry merit
The plant of energy, microorganism are arisen at the historic moment, and have promoted further application and the development of bioremediation technology.At oil-polluted soils
Governance process in, on the one hand to improve bioremediation technology further so that it is maturing and systematization;On the other hand it is noted that
With the combination of traditional method, play respective advantage.
The most single to the means of the process of oil pollution, the main single recovery technique used, single
The biological restoration of one or single chemical method.Although single bioremediation technology is environmentally friendly, but biology is degeneration-resistant
Property environment in laundering period the longest, therefore process the best at Polluted area urgently to be resolved hurrily.Therefore for reparation merit can be made
Imitating the most satisfied, reparation result is the most thorough, and the chemical repair method of reaction is requisite rapidly.Skill is repaired in chemical treatment
The application of art success first consists in the selection of suitable inorganic agent.The most conventional chemical treatments has hydrogen peroxide, Gao Meng
Hydrochlorate, Fenton reagent, modified Fenton reaction and activation persulfate etc..Additionally, Watts etc. (1999) report the strongest place
Reason condition, the H of excess2O2Desorbing and the process of the organic pollution adsorbed can be improved by producing other free radical,
Dadkhah&Akgerman (2006) point out use subcritical water as remove medium and cross process hydrogen as inorganic agent, 30 points
After clock experiment, liquid does not have PAHs to be detected.The most existing company, as REGEBESIS and I-ROX is also concentrating on
The research of chemical treatment degraded underground water pollution.But, chemical treatment method also has its limitation, such as, processing degradation process
In, inorganic agent can process and change the adsorption structure in soil to other organic matter in soil, and biology can
Usability, is unfavorable for the growth of plant and microorganism, it is impossible to be effectively used in combination with bioremediation technology.
For improving the efficiency repaired further, solve the engagement technique problem between chemical oxidation and biological treatment, this
Bright propose a kind of Combined Treatment technology based on the biological reinforced strategy of sodium peroxydisulfate oxidation processes and flora, to severe stone
Soil contaminated by crude oil or greasy filth are efficiently treated through.
Summary of the invention
It is an object of the invention to provide a kind of biological reinforced with microorganism species based on the oxidation of sodium peroxydisulfate complex
Conjunction processes soil with serious petroleum pollution or the method for greasy filth, and the method can significantly increase soil with serious petroleum pollution or oil in pole
The treatment effeciency of mud, and the process cycle can be greatly shortened, combine sodium peroxydisulfate oxidation processes substrate selective low, efficient,
Cycle is short and flora is biological reinforced processes gentle, the environment amenable plurality of advantages of converted product, can be at contaminated soil or contain
Greasy dirt pollutes on-the-spot original position or dystopy processes soil with serious petroleum pollution or greasy filth.
Biological reinforced combination treatment method involved in the present invention is to use bacillus cereus (Bacillus sp.) and branch top
Spore mycete (Acremonium sp.) or the fungus-bacteria flora solid of Phanerochaete chrysosporium (Phanerochaete sp.)
Microbial inoculum accesses in soil with serious petroleum pollution or greasy filth.
Bacterium involved in the present invention is respectively bacillus cereus, branch acremonium bacterium and Phanerochaete chrysosporium, wherein: spore bar
Bacterium Bacillus sp., bacterial strain preserving number CCTCC NO:AB 2014248, belong to known solid fungicide, and this bacterium bacterium colony is milky white
Color, bacterium colony is circular, smooth surface, projection, neat in edge, and back side color is consistent with front, and non-pigment is distributed.Branch acremonium bacterium
Acremonium sp.Y0997, bacterial strain preserving number is CCTCC NO:M 2016117, and depositary institution is that Chinese Typical Representative culture is protected
Center, Tibetan, address: Wuhan, China Wuhan University, postcode: 430072, preservation date is on March 14th, 2016, and this bacterium bacterium colony is in vain
Color, bacterium colony is circular, and the back side is cream-colored to ochre yellow, hyphae colorless, smooth and tool barrier film, and sporangiophore is directly long from mycelia
Going out, tapered many Dan Sheng, conidium is colourless, smooth, forms tufted or chain, long spindle, Rhizoma Anemones Raddeanae, has no thick wall spore
Son.Phanerochaete chrysosporium Phanerochaete sp.F0996, bacterial strain preserving number is CCTCC NO:M 2016116, preservation list
Position is China typical culture collection center, and address: Wuhan, China Wuhan University, postcode: 430072, preservation date is 2016
March 14, this bacterium bacteria colony white, circular, the back side is colourless, and bacterium colony only has a small amount of protruding;Mycelia is colourless, fluffy flocculence, with
The prolongation of incubation time, fine hair shape projection gradually flattens, and mycelia disappears, and forms aleuriospore.
To achieve these goals, the technical solution adopted in the present invention comprises the steps of:
(1) gather soil with serious petroleum pollution or greasy filth is placed in process pond, broken into pieces, mix, or directly will pollute
On-the-spot soil with serious petroleum pollution or greasy filth are broken into pieces, are mixed;
(2) sodium peroxydisulfate complex is added, in the ratio of 8~15g/kg, the soil processed through step (1) as oxidant
Or in greasy filth, and the moisture content adding water to soil or greasy filth reaches 15~30%, mixes, oxidation processes 10~20 days;
(3) soil after step (2) processes or greasy filth are respectively connected to cultured bacillus cereus and branch top born of the same parents mycete
Mix bacterium agent or the mix bacterium agent of bacillus cereus and Phanerochaete chrysosporium, bacillus cereus and branch acremonium bacterium or yellow archespore
The inoculum concentration of the flat lead fungi of hair is 15~25g/kg, adds organic fertilizer, and the addition of organic fertilizer is 800~1200mg/
Kg, mixing, by the surface covered rearing with plastic film of contaminated soil or greasy filth, ventilation, the moisture content maintaining soil or greasy filth is stable,
At 15~35 DEG C, in-situ processing 10~20 days;
(4) soil after step (3) processes or greasy filth access the cultured bacillus cereus of equivalent and branch top born of the same parents again
The mix bacterium agent of mycete or bacillus cereus and the mix bacterium agent of Phanerochaete chrysosporium, repeat the operation of step (3), 15~
At 35 DEG C, in-situ processing 20~30 days, complete to process.
Above-mentioned sodium peroxydisulfate complex is accessed in the soil or greasy filth processed through step (1) in the ratio of 8~15g/kg, then
Adding biological or nontoxic surfactants, the addition of surfactant is 1200~1600mg/kg, and adds water to soil or oil
The moisture content of mud reaches 15~30%, mixes, oxidation processes 10~20 days;
Above-mentioned sodium peroxydisulfate complex is mixed with the mol ratio of 5:0.5:2.5 by sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate
Conjunction is made.
The entitled Acremonium sp.Y0997 of above-mentioned branch acremonium bacterium, bacterial strain preserving number is CCTCC NO:M
2016117, preservation date is on March 14th, 2016, and depositary institution is China typical culture collection center;Yellow archespore Mao Pingge
The entitled Phanerochaete sp.F0996 of bacterium, bacterial strain preserving number is CCTCC NO:M 2016116, during depositary institution is
State's Type Tissue Collection, preservation date is on March 14th, 2016.
The viable bacteria content of above-mentioned bacillus cereus is 4.0 × 109~6.0 × 109CFU/g, the viable bacteria content of branch acremonium bacterium is
7.2×109~8.5 × 109CFU/g, the viable bacteria content of Phanerochaete chrysosporium is 7.2 × 109~9.0 × 109CFU/g。
The total petroleum hydrocarbon initial content of above-mentioned soil with serious petroleum pollution or greasy filth is 100000~170000mg/kg soil
Or greasy filth.
The present invention is based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment serious petroleum pollution
Soil or the method for greasy filth, it is the most double after oxidized dose of elder generation carries out chemical oxidation treatment with surfactant cooperation connecing
The mixed bacterial entering bacillus cereus and branch acremonium bacterium or Phanerochaete chrysosporium carries out the biological reinforced reparation of sequence, by chemical oxygen
Change technology recovery technique biological reinforced with the sequence of fungus-bacteria flora is combined, it is possible to the degraded effect of pole raising TPH significantly
Rate, and substantially reduce repairing efficiency, combine that sodium peroxydisulfate oxidation processes substrate selective is low, efficient, the cycle is short and
Flora is biological reinforced processes gentle, the environment amenable plurality of advantages of converted product, can pollute at contaminated soil or oily sludge
On-the-spot original position or dystopy process soil with serious petroleum pollution or greasy filth.To the crude quality serious petroleum pollution at concentrations up to 17%
The treatment effect of soil or greasy filth is obvious, and result of the test shows: after processing 10~20 days, TPH degradation efficiency in soil or greasy filth
It is 30%~50%, the degradation efficiency of wherein light chain Yu heavy chain component has all been reached more than 35%, respectively 38.53 ±
1.06%, 42.78 ± 1.45%, by bacillus sp. subsequently and branch acremonium bacterium Acremonium sp.'s
After the flora process of 35~50 days by a definite date, in soil or greasy filth, TPH degradation efficiency has reached 86%~95%, the flora of the present invention
Growth is stable, and branch acremonium bacterium and Phanerochaete chrysosporium are preferable to the tolerance of outdoor elements at the scene, survival rate
Higher, further it is proposed that technical system can apply to completely petroleum-type compound pollute medium such as Polluted Soil
The production garbage of earth or oily sludge and shale gas collection site or petroleum wastewater etc., and had in oil producing operation district
On-site verification, reliable and stable, also can operate with petroleum-type compound pollute medium such as contaminated soil or pollutant effluents with
And the production garbage of shale gas collection site or petroleum wastewater etc..
Accompanying drawing explanation
Fig. 1 is the bacterium colony figure of branch acremonium bacterium (Acremonium sp.Y0997).
Fig. 2 is the shape appearance figure of Phanerochaete chrysosporium (Phanerochaete sp.F0996).
Fig. 3 is that the internal standard method for gas chromatography processing 15 days blank example soil Petroleum Hydrocarbon components analyzes collection of illustrative plates.
Fig. 4 is the internal standard method for gas chromatography analysis of 15 days embodiment 1 soil Petroleum Hydrocarbon components of chemical oxidation treatment
Collection of illustrative plates.
Fig. 5 is light chain and the removal efficiency result of heavy chain component in 15 days embodiment 1 soil of chemical oxidation treatment.
Fig. 6 is for processing 60 days blank examples and comparative example and TPH removal efficiency comparing result in embodiment 1 soil.
Detailed description of the invention
In conjunction with test and embodiment, technical scheme is further described, but the present invention is not limited only to
Embodiment disclosed below.
Embodiment 1
Now with bacillus cereus, (Bacillus sp. is preserved in China typical culture collection center, bacterial strain preserving number
CCTCC NO:AB2014248) and branch acremonium bacterium (Acremonium sp.Y0997, is preserved in China typical culture collection
Center, bacterial strain preserving number is CCTCC NO:M 2016117, and preservation date is on March 14th, 2016) solid fungicide be flora
Combination, with sodium peroxydisulfate complex as chemical oxidizing agent, combines and processes soil with serious petroleum pollution, by following steps group
Become:
(1) gather the soil with serious petroleum pollution that total petroleum hydrocarbon initial content is 162240mg/kg near oil well to be placed in
Processing in pond, broken into pieces, particle diameter is 30~about 40mm, mixing;
(2) oxidant sodium peroxydisulfate complex is accessed in the soil that step (1) processes in the ratio of 10g/kg, add water
Moisture content to soil reaches 25%, and mixing, oxidation processes 15 days, this sodium peroxydisulfate complex is by sodium peroxydisulfate, calper calcium peroxide
Make with the mixed in molar ratio of 5:0.5:2.5 with ferrous sulfate;
(3) cultured bacillus cereus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.) are accessed weight
In degree oil-polluted soils, the inoculum concentration of bacillus cereus is 20g/kg soil (its solid fungicide viable bacteria content: 5.3 × 109CFU/
G), the inoculum concentration of branch acremonium bacterium is 20g/kg soil (its solid fungicide viable bacteria content: 8.0 × 109CFU/g), according still further to
The amount of 1000mg/kg (soil) adds organic fertilizer, mixing, and the moisture content maintaining soil is stable, will process the surface in pond with moulding
Material thin film covers, ventilation, and the moisture content maintaining soil is stable, at 25 DEG C, and in-situ processing 15 days;
(4) soil after step (3) processes accesses the cultured bacillus cereus of equivalent and branch acremonium bacterium again, weight
The operation of multiple step (3), at 25 DEG C, in-situ processing 30 days, complete to process, the degradation rate of TPH reaches 86.96%.
The 18SrDNA sequence of above-mentioned branch acremonium bacterium (Acremonium sp.Y0997) is:
This bacterium bacterium colony is white, and bacterium colony is circular, and the back side is cream-colored to ochre yellow, hyphae colorless, smooth and tool barrier film, spore
Upright the connecing of capsule grows from mycelia, tapered many Dan Sheng, and conidium is colourless, smooth, formation tufted or chain, long spindle,
Rhizoma Anemones Raddeanae, has no that chlamydospore, pattern see Fig. 1.
Embodiment 2
Now with Phanerochaete chrysosporium, (Phanerochaete sp.F0996, is preserved in China typical culture collection
The heart, bacterial strain preserving number CCTCC NO:M 2016116, preservation date is on March 14th, 2016) solid fungicide be flora composition,
With sodium peroxydisulfate complex as chemical oxidizing agent, combine and soil with serious petroleum pollution processed, comprise the steps of:
(1) gather the soil with serious petroleum pollution that total petroleum hydrocarbon initial content is 170000mg/kg near oil well to be placed in
Processing in pond, broken into pieces, particle diameter is 30~about 40mm, mixing;
(2) oxidant sodium peroxydisulfate complex is accessed in the soil that step (1) processes in the ratio of 8g/kg, add water
Moisture content to soil reaches 15%, and mixing, oxidation processes 20 days, sodium peroxydisulfate complex used is by sodium peroxydisulfate, peroxidating
Calcium and ferrous sulfate are made with the mixed in molar ratio of 5:0.5:2.5;
(3) by cultured bacillus cereus (Bacillus sp.) and Phanerochaete chrysosporium (Phanerochaete
Sp.F0996) accessing in soil with serious petroleum pollution, the inoculum concentration of bacillus cereus is that (its solid fungicide viable bacteria contains 15g/kg soil
Amount: 4.0 × 109CFU/g), the inoculum concentration of Phanerochaete chrysosporium be 15g/kg soil (its solid fungicide viable bacteria content: 7.5 ×
109CFU/g), the amount according still further to 800mg/kg (soil) adds organic fertilizer, mixing, and the moisture content maintaining soil is stable, will at
The surface covered rearing with plastic film in reason pond, ventilation, the moisture content maintaining soil is stable, at 15 DEG C, in-situ processing 20 days;
(4) soil after step (3) processes accesses the cultured bacillus cereus of equivalent and yellow archespore Mao Pingge again
The mix bacterium agent of bacterium, repeats the operation of step (3), at 15 DEG C, in-situ processing 30 days, completes to process, and the degradation rate of TPH reaches
85.25%.
Above-mentioned Phanerochaete chrysosporium Phanerochaete sp.F0996, bacterial strain preserving number is CCTCC NO:M
2016116, depositary institution is China typical culture collection center, and preservation date is on March 14th, 2016, its 18SrDNA sequence
It is classified as:
This bacterium bacteria colony white, circular, the back side is colourless, and bacterium colony only has a small amount of protruding;Mycelia is colourless, fluffy flocculence, with
The prolongation of incubation time, fine hair shape projection gradually flattens, and mycelia disappears, and forms aleuriospore, sees Fig. 2.
Embodiment 3
With sodium peroxydisulfate complex as chemical oxidizing agent, associating bacillus cereus and branch acremonium bacterium mixed bacterial are to severe stone
Greasy dirt dye greasy filth carries out strengthening Combined Treatment, comprises the steps of:
(1) gather the serious petroleum pollution greasy filth that total petroleum hydrocarbon initial content is 100000mg/kg near oil well to be placed in
Processing in pond, broken into pieces, particle diameter is 30~about 40mm, mixing;
(2) oxidant sodium peroxydisulfate complex is accessed in the greasy filth that step (1) processes in the ratio of 12g/kg, over cure
Acid sodium complex is made up with the mixed in molar ratio of 5:0.5:2.5 of sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate, according still further to
The addition addition heavy oil cleaning agent KD-L315 of 1200mg/kg (greasy filth) is as surfactant, and adds water to the aqueous of greasy filth
Rate reaches 30%, mixes, oxidation processes 10 days;
(3) cultured bacillus cereus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.) are accessed weight
In degree oil pollution greasy filth, the inoculum concentration of bacillus cereus is 25g/kg greasy filth (its solid fungicide viable bacteria content: 6.0 × 109CFU/
G), the inoculum concentration of branch acremonium bacterium is 25g/kg greasy filth (its solid fungicide viable bacteria content: 8.5 × 109CFU/g), according still further to
The amount of 1200mg/kg (greasy filth) adds organic fertilizer, mixing, and the moisture content maintaining greasy filth is stable, will process the surface in pond with moulding
Material thin film covers, ventilation, and the moisture content maintaining greasy filth is stable, at 35 DEG C, and in-situ processing 10 days;
(4) greasy filth after step (3) processes accesses the cultured bacillus cereus of equivalent and branch acremonium bacterium again, weight
The operation of multiple step (3), at 35 DEG C, in-situ processing 20 days, complete to process, the degradation rate of TPH reaches 95.01%.
Embodiment 4
With sodium peroxydisulfate complex as chemical oxidizing agent, associating bacillus cereus and Phanerochaete chrysosporium mixed bacterial counterweight
Degree oil pollution greasy filth carries out strengthening Combined Treatment, comprises the steps of:
(1) gather the serious petroleum pollution greasy filth that total petroleum hydrocarbon initial content is 120000mg/kg near oil well to be placed in
Processing in pond, broken into pieces, particle diameter is 30~about 40mm, mixing;
(2) oxidant sodium peroxydisulfate complex is accessed in the greasy filth that step (1) processes in the ratio of 15g/kg, over cure
Acid sodium complex is made up with the mixed in molar ratio of 5:0.5:2.5 of sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate, according still further to
The addition of 1600mg/kg (greasy filth) adds heavy oil cleaning agent KD-L315 as surfactant, or other commercially available biologies or
Nontoxic surfactants, and the moisture content adding water to greasy filth reaches 30%, mixing, oxidation processes 10 days;
(3) by cultured bacillus cereus (Bacillus sp.) and Phanerochaete chrysosporium (Phanerochaete
Sp.) mix bacterium agent accesses in serious petroleum pollution greasy filth, and the inoculum concentration of bacillus cereus is 25g/kg greasy filth (its solid fungicide
Viable bacteria content: 5.0 × 109CFU/g), the inoculum concentration of Phanerochaete chrysosporium is that (its solid fungicide viable bacteria contains 25g/kg greasy filth
Amount: 9.0 × 109CFU/g), the amount according still further to 1200mg/kg (greasy filth) adds organic fertilizer, mixing, maintains the moisture content of greasy filth
Stable, the surface covered rearing with plastic film in pond, ventilation will be processed, the moisture content maintaining greasy filth is stable, at 35 DEG C, in-situ processing
10 days;
(4) greasy filth after step (3) processes accesses the cultured bacillus cereus of equivalent and yellow archespore Mao Pingge again
Bacterium, repeats the operation of step (3), at 35 DEG C, in-situ processing 20 days, completes to process, and the degradation rate of TPH reaches 93.31%.
The method of the present invention is the most applicable to process soil with serious petroleum pollution and greasy filth, it is also possible to directly processes and pollutes now
The soil with serious petroleum pollution of field or greasy filth, all can obtain good treatment effect.
Above-mentioned branch top born of the same parents mycete can mutually be replaced with Phanerochaete chrysosporium, and it is mixed to form very with bacillus cereus
Bacterium-antibacterial mix bacterium agent, all has preferable effect, particularly with oxidant oxygen to above-mentioned soil with serious petroleum pollution and greasy filth
Change Combined Treatment.
In order to verify the technique effect of the present invention, applicant has done substantial amounts of experiment, the most also using blank experiment as ginseng
According to, the degradation effect of the present invention is verified, specific as follows:
Comparative example
(1) total petroleum hydrocarbon initial content is that 162240mg/kg soil with serious petroleum pollution is placed in process pond, is struck
Broken, mixing;
(2) identical with the step of embodiment 1 (3).
(3) identical with the step of embodiment 1 (4).
Blank example:
(1) identical with comparative example 1;
(2) in the contaminated soil that total petroleum hydrocarbon initial content is 162240mg/kg, organic fertilizer and surface activity are added
Agent, and the moisture content adding water to soil reaches 25%, mixing, at 15~35 DEG C, in-situ processing 60 days, complete to process.
In soil after processing above-mentioned blank example and embodiment 1 15 days, total petroleum hydrocarbon component carries out gas chromatogram and mass spectrum
Combination detection, shown in result below figure 3 and Fig. 4.
Shown by result in Fig. 3 and 4, compared with blank example, after sodium peroxydisulfate complex oxidation processes, each in soil
Petroleum hydrocarbon component all has obvious degradation effect, and carries out the removal efficiency of light chain in soil after oxidation processes Yu heavy chain component
Calculating, result is as shown in Figure 5.As it can be seen, after sodium peroxydisulfate complex oxidation processes, the light chain in soil and heavy chain component
Removal efficiency respectively reached 38.53 ± 1.06%, 42.78 ± 1.45%.
The degraded situation of total petroleum hydrocarbon in soil after handled by above-mentioned comparative example and blank example is detected, and with reality
Executing example 1 to compare, result is as shown in table 1 below and Fig. 6.
Table 1 is TPH content and the comparing result of embodiment 1 in the soil after comparative example and blank example process
TPH content and the contrast of embodiment 1 in soil after being processed 60 days from table 1 and Fig. 6, comparative example and blank example
Result shows, uses the embodiment 1 of chemical oxidation pre-treatment the most significantly to improve the TPH removal efficiency in soil, with contrast
Example is compared, and the TPH removal efficiency of embodiment 1 improves about 22.54%.
It is also carried out processing to the greasy filth of serious petroleum pollution by identical method, it is also possible to reach above-mentioned identical process effect
Really.
Result of the test shows, the present invention uses chemical oxidation and the biological reinforced comprehensive in-situ processing weight of fungus-bacteria flora
Degree oil-polluted soils or greasy filth, can significantly increase the degradation efficiency of total petroleum hydrocarbon in soil or greasy filth in pole.
Claims (6)
1. one kind aoxidizes and microorganism species biological reinforced Combined Treatment soil with serious petroleum pollution based on sodium peroxydisulfate complex
Or the method for greasy filth, it is characterised in that comprise the steps of:
(1) gather soil with serious petroleum pollution or greasy filth is placed in process pond, broken into pieces, mix, or directly will pollute scene
Soil with serious petroleum pollution or greasy filth break into pieces, mix;
(2) sodium peroxydisulfate complex is added, as oxidant, the soil or oil processed through step (1) in the ratio of 8~15g/kg
In mud, and the moisture content adding water to soil or greasy filth reaches 15~30%, mixes, oxidation processes 10~20 days;
(3) soil after step (2) processes or greasy filth are respectively connected to the mixed of cultured bacillus cereus and branch top born of the same parents mycete
Close the mix bacterium agent of microbial inoculum or bacillus cereus and Phanerochaete chrysosporium, bacillus cereus and branch acremonium bacterium or yellow archespore hair to put down
The inoculum concentration of lead fungi is 15~25g/kg, adds organic fertilizer, and the addition of organic fertilizer is 800~1200mg/kg, mixed
Even, by the surface covered rearing with plastic film of contaminated soil or greasy filth, ventilation, the moisture content maintaining soil or greasy filth is stable, 15
~at 35 DEG C, in-situ processing 10~20 days;
(4) soil after step (3) processes or greasy filth access the cultured bacillus cereus of equivalent and branch top born of the same parents mycete again
Mix bacterium agent or the mix bacterium agent of bacillus cereus and Phanerochaete chrysosporium, repeat the operation of step (3), at 15~35 DEG C
Under, in-situ processing 20~30 days, complete to process.
The most according to claim 1 based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment weight
Degree oil-polluted soils or the method for greasy filth, it is characterised in that described sodium peroxydisulfate complex is accessed in the ratio of 8~15g/kg
In the soil processed through step (1) or greasy filth, add surfactant, the addition of surfactant be 1200~
1600mg/kg, and the moisture content adding water to soil or greasy filth reaches 15~30%, mixing, oxidation processes 10~20 days.
The most according to claim 1 and 2 biological reinforced with microorganism species combine place based on the oxidation of sodium peroxydisulfate complex
Reason soil with serious petroleum pollution or the method for greasy filth, it is characterised in that: described sodium peroxydisulfate complex is by sodium peroxydisulfate, peroxidating
Calcium and ferrous sulfate are made with the mixed in molar ratio of 5:0.5:2.5.
The most according to claim 1 based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment weight
Degree oil-polluted soils or the method for greasy filth, it is characterised in that: the entitled Acremonium of described branch acremonium bacterium
Sp.Y0997, bacterial strain preserving number is CCTCC NO:M 2016117, and preservation date is on March 14th, 2016, during depositary institution is
State's Type Tissue Collection;
The entitled Phanerochaete sp.F0996 of described Phanerochaete chrysosporium, bacterial strain preserving number is CCTCC NO:M
2016116, depositary institution is China typical culture collection center, and preservation date is on March 14th, 2016.
The most according to claim 1 based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment weight
Degree oil-polluted soils or the method for greasy filth, it is characterised in that: the viable bacteria content of described bacillus cereus is 4.0 × 109~6.0 ×
109CFU/g, the viable bacteria content of branch acremonium bacterium is 7.2 × 109~8.5 × 109CFU/g, the viable bacteria of Phanerochaete chrysosporium contains
Amount is 7.2 × 109~9.0 × 109CFU/g。
The most according to claim 1 based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment weight
Degree oil-polluted soils or the method for greasy filth, it is characterised in that: the total petroleum hydrocarbon of described soil with serious petroleum pollution or greasy filth rises
Beginning content is 100000~170000mg/kg soil or greasy filth.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610541245.4A CN106190891B (en) | 2016-07-11 | 2016-07-11 | Method based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610541245.4A CN106190891B (en) | 2016-07-11 | 2016-07-11 | Method based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106190891A true CN106190891A (en) | 2016-12-07 |
CN106190891B CN106190891B (en) | 2019-10-25 |
Family
ID=57473087
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610541245.4A Active CN106190891B (en) | 2016-07-11 | 2016-07-11 | Method based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106190891B (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106623405A (en) * | 2016-12-22 | 2017-05-10 | 常州大学 | Method for remediation of benzene hexachloride contaminated soil by Fe (II) / CaO2 |
CN106734164A (en) * | 2016-12-22 | 2017-05-31 | 常州大学 | A kind of microwave reinforced nanometer Fe3O4/Na2CO4The method for repairing PAEs contaminated soils |
CN106811205A (en) * | 2016-12-21 | 2017-06-09 | 中国石油天然气集团公司 | Persulfate coupling indigenous microorganism stimulates the method for repairing petroleum hydrocarbon contaminated soil |
CN107214189A (en) * | 2017-07-19 | 2017-09-29 | 河北煜环环保科技有限公司 | A kind of organic material contaminated soil renovation technique |
CN108435786A (en) * | 2018-05-07 | 2018-08-24 | 句容宇发农业发展有限公司 | A method of repairing pesticide contaminated soil |
CN108580539A (en) * | 2018-04-18 | 2018-09-28 | 浙江大学 | A kind of method of chemical oxidation coupling microorganism remediation organic polluted soil |
CN108892353A (en) * | 2018-09-19 | 2018-11-27 | 新疆科力新技术发展股份有限公司 | Oil field mining liquid central treating station process for treating oil-containing sludge and device |
CN108941191A (en) * | 2018-07-03 | 2018-12-07 | 上海松沅环境修复技术有限公司 | A kind of repairing method of microorganism of oil-polluted soils |
CN109095740A (en) * | 2018-10-15 | 2018-12-28 | 中石化石油工程技术服务有限公司 | Oily sludge method for innocent treatment |
CN109759431A (en) * | 2019-01-25 | 2019-05-17 | 湖南新九方科技有限公司 | A kind of restorative procedure of gas station's contaminated site |
CN111922065A (en) * | 2020-07-31 | 2020-11-13 | 中冶华天工程技术有限公司 | Method for restoring organic contaminated soil by combining chemical oxidation with microorganisms |
CN113770155A (en) * | 2021-08-20 | 2021-12-10 | 鄂尔多斯市昊鑫绿科环境工程有限公司 | High-temperature oxidation treatment method for drilling solid waste of oil and gas field |
CN113787093A (en) * | 2021-09-14 | 2021-12-14 | 中国科学院武汉岩土力学研究所 | Method for repairing organic polluted soil by activating sodium persulfate through carbide slag loaded with bacillus |
CN115572031A (en) * | 2022-10-28 | 2023-01-06 | 中南大学 | Method for treating oily sludge by combining biological treatment and chemical treatment |
USD1026013S1 (en) * | 2021-08-11 | 2024-05-07 | Beijing Kuaimajiabian Technology Co., Ltd. | Display screen or portion thereof with an animated graphical user interface |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102794296A (en) * | 2012-08-01 | 2012-11-28 | 中国石油天然气股份有限公司 | Complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and restoring method thereof |
CN104031870A (en) * | 2014-06-24 | 2014-09-10 | 西北民族大学 | Microbial compound bacterial agent and soil combined remediation agent prepared from microbial compound bacterial agent and application of microbial compound bacterial agent and soil combined remediation agent |
CN105170644A (en) * | 2015-10-30 | 2015-12-23 | 李磊 | Bioremediation method for petroleum-polluted soil |
-
2016
- 2016-07-11 CN CN201610541245.4A patent/CN106190891B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102794296A (en) * | 2012-08-01 | 2012-11-28 | 中国石油天然气股份有限公司 | Complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and restoring method thereof |
CN104031870A (en) * | 2014-06-24 | 2014-09-10 | 西北民族大学 | Microbial compound bacterial agent and soil combined remediation agent prepared from microbial compound bacterial agent and application of microbial compound bacterial agent and soil combined remediation agent |
CN105170644A (en) * | 2015-10-30 | 2015-12-23 | 李磊 | Bioremediation method for petroleum-polluted soil |
Non-Patent Citations (4)
Title |
---|
XIAO-KUI MA ET AL.: "Bioaugmentation of soil contaminated with high-level crude oil through inoculation with mixed cultures including Acremonium sp.", 《BIODEGRADATION》 * |
吴昊 等: "CaO2/Fe2+活化过硫酸钠对石油类污染土壤的修复效果", 《环境科学》 * |
吴昊 等: "活化过硫酸钠原位修复石油类污染土壤研究进展", 《环境化学》 * |
谭丽泉 等: "黄孢原毛平革菌对土壤中石油的降解研究", 《安徽农业科学》 * |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106811205B (en) * | 2016-12-21 | 2020-04-10 | 中国石油天然气集团公司 | Method for restoring petroleum hydrocarbon polluted soil by persulfate coupled indigenous microorganism stimulation |
CN106811205A (en) * | 2016-12-21 | 2017-06-09 | 中国石油天然气集团公司 | Persulfate coupling indigenous microorganism stimulates the method for repairing petroleum hydrocarbon contaminated soil |
CN106734164A (en) * | 2016-12-22 | 2017-05-31 | 常州大学 | A kind of microwave reinforced nanometer Fe3O4/Na2CO4The method for repairing PAEs contaminated soils |
CN106623405A (en) * | 2016-12-22 | 2017-05-10 | 常州大学 | Method for remediation of benzene hexachloride contaminated soil by Fe (II) / CaO2 |
CN107214189A (en) * | 2017-07-19 | 2017-09-29 | 河北煜环环保科技有限公司 | A kind of organic material contaminated soil renovation technique |
CN108580539A (en) * | 2018-04-18 | 2018-09-28 | 浙江大学 | A kind of method of chemical oxidation coupling microorganism remediation organic polluted soil |
CN108435786A (en) * | 2018-05-07 | 2018-08-24 | 句容宇发农业发展有限公司 | A method of repairing pesticide contaminated soil |
CN108941191A (en) * | 2018-07-03 | 2018-12-07 | 上海松沅环境修复技术有限公司 | A kind of repairing method of microorganism of oil-polluted soils |
CN108892353A (en) * | 2018-09-19 | 2018-11-27 | 新疆科力新技术发展股份有限公司 | Oil field mining liquid central treating station process for treating oil-containing sludge and device |
CN109095740A (en) * | 2018-10-15 | 2018-12-28 | 中石化石油工程技术服务有限公司 | Oily sludge method for innocent treatment |
CN109759431A (en) * | 2019-01-25 | 2019-05-17 | 湖南新九方科技有限公司 | A kind of restorative procedure of gas station's contaminated site |
CN111922065A (en) * | 2020-07-31 | 2020-11-13 | 中冶华天工程技术有限公司 | Method for restoring organic contaminated soil by combining chemical oxidation with microorganisms |
USD1026013S1 (en) * | 2021-08-11 | 2024-05-07 | Beijing Kuaimajiabian Technology Co., Ltd. | Display screen or portion thereof with an animated graphical user interface |
CN113770155A (en) * | 2021-08-20 | 2021-12-10 | 鄂尔多斯市昊鑫绿科环境工程有限公司 | High-temperature oxidation treatment method for drilling solid waste of oil and gas field |
CN113787093A (en) * | 2021-09-14 | 2021-12-14 | 中国科学院武汉岩土力学研究所 | Method for repairing organic polluted soil by activating sodium persulfate through carbide slag loaded with bacillus |
CN115572031A (en) * | 2022-10-28 | 2023-01-06 | 中南大学 | Method for treating oily sludge by combining biological treatment and chemical treatment |
Also Published As
Publication number | Publication date |
---|---|
CN106190891B (en) | 2019-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106190891A (en) | Based on the oxidation of sodium peroxydisulfate complex and microorganism species biological reinforced Combined Treatment soil with serious petroleum pollution or the method for greasy filth | |
CN105170644B (en) | A kind of biological renovation method of oil-polluted soils | |
CN106077076B (en) | A method of based on SODIUM PERCARBONATE oxidation and microbial augmentation integrated treatment soil with serious petroleum pollution or greasy filth | |
CN109570220B (en) | Method for restoring arsenic-polluted soil by bacteria-induced active iron manganese oxide under aerobic/microaerobic conditions | |
Singh | Biodegradation of synthetic dyes: a mycoremediation approach for degradation/decolourization of textile dyes and effluents | |
Tišma et al. | White-rot fungi in phenols, dyes and other xenobiotics treatment–a brief review | |
CN108130299B (en) | Microbial strain for degrading petroleum hydrocarbon and combined repairing agent | |
CN105032908B (en) | The device and process of a kind of renovation of organic pollution soil and underground water | |
CN110283772A (en) | A kind of preparation method of functional flora that repairing petroleum hydrocarbon contaminated soil and underground water | |
CN104438283B (en) | A kind of method utilizing microorganism to accelerate aerobic degradation urban solid garbage refuse | |
CN110204030A (en) | A method of utilizing trivalent arsenic in biological char combustion underground water | |
CN103224282A (en) | Method and special equipment for treating oil field wastewater | |
CN103484447A (en) | Preparation method and application of petroleum degrading enzyme preparation | |
Jenkins et al. | Antimony biomethylation by mixed cultures of micro‐organisms under anaerobic conditions | |
CN108405600A (en) | A kind of method of sodium peroxydisulfate and hydrogen peroxide compound system in-situ immobilization organic pollution soil | |
CN112570444A (en) | Low-temperature heat-enhanced chemical oxidation-microorganism coupling repair system and method for polycyclic aromatic hydrocarbon contaminated soil | |
CN102847705A (en) | Restoration method for degrading soil PCP residue through cooperation of earthworms and microbes | |
CN108188162A (en) | A kind of serious pollution land method | |
CN105505794A (en) | Schizophyllum strain and application thereof | |
CN108114978B (en) | A kind of method of the efficient rehabilitating soil of chemistry-microorganism | |
CN107311401B (en) | Method for removing organic matters in water | |
CN113695369A (en) | Method for in-situ remediation of petroleum hydrocarbon contaminated soil through multi-technology coupling | |
CN105820963A (en) | Phanerochaete chrysosporium and application thereof | |
CN105524889A (en) | Preparation method of enzyme preparation for polycyclic aromatic hydrocarbon contaminated soil remediation | |
CN210915496U (en) | Volatile organic compounds repair system in groundwater |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |