CN106190891B - Method based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth - Google Patents
Method based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth Download PDFInfo
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Abstract
The invention discloses a kind of methods based on sodium peroxydisulfate compound oxidation and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora or greasy filth, it is used cooperatively first with sodium peroxydisulfate compound as oxidant and surfactant and oxidation processes is carried out to contaminated soil or greasy filth, access the mixed bacterial of bacillus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.Y0997) or Phanerochaete chrysosporium (Phanerochaete sp.F0996) twice in succession later, combined by chemical oxidation with fungi-bacteria flora biological sequence reinforcing and reinforcing Combined Treatment is carried out to soil or greasy filth, it has been remarkably improved the degradation efficiency of soil with serious petroleum pollution or greasy filth, and it is substantially shorter repairing efficiency.
Description
Technical field
The invention belongs to soil with serious petroleum pollution or the chemistry and technical field of biological remediation of greasy filth, and in particular to oil
Field mining area utilizes chemical oxidation and the biological reinforced reinforcing Combined Treatment soil with serious petroleum pollution of fungi-bacteria flora or greasy filth
Method.
Technical background
Currently, oil-polluted soils range constantly expands with the development of domestic and international petroleum industry.For petroleum wastewater
Dye soil is handled, and is made it achieve the effect that restore to utilize in a relatively short period of time, is in oil-polluted soils governance process
Urgent problem to be solved.Which method optimum of processing to(for) pollutant selects, in addition to consider handling pollutant location
Point, amount of pollutant number, outside the technical factors such as length of the quality for the treatment of effect, the length of required time and processing, processing
The height of expense is a highly important factor.
In recent years, bioremediation technology has at home and abroad all obtained faster development, and a batch has special physiological biochemistry function
Plant, the microorganism of energy are come into being, and the further application and development of bioremediation technology have been pushed.In oil-polluted soils
Governance process in, on the one hand further to improve bioremediation technology, make its maturing and systematization；On the other hand it is noted that
And the combination of conventional method plays respective advantage.
Current relatively simple to the means of the processing of oil pollution both at home and abroad, the single recovery technique mainly used, it is single
One biological prosthetic or single chemical method.Single bioremediation technology is although environmentally friendly, and biology is degeneration-resistant
Property environment in laundering period it is often longer, therefore in Polluted area processing urgently to be resolved and bad.It therefore is that can make to repair function
Effect is more satisfied, and reparation result is more thorough, and the chemical repair method rapidly reacted is essential.Skill is repaired in chemical treatment
Art success application first consists in the selection of suitable inorganic agent.Currently used chemical treatments have hydrogen peroxide, Gao Meng
Hydrochlorate, Fenton reagent, modified Fenton reaction and activation persulfate etc..In addition, Watts etc. (1999) reports very strong place
Manage bar part, excessive H2O2The desorption and processing that the organic pollutant of absorption can be improved by generating other free radical,
Dadkhah&Akgerman (2006) points out to use subcritical water as removal medium and crosses processing hydrogen as inorganic agent, and 30 points
There is no PAHs that can be detected in liquid after clock experiment.External existing company, as REGEBESIS and I-ROX is also being concentrated on
The research of chemical treatment degradation underground water pollution.But chemical treatment method also has its limitation, such as in processing degradation process
In, inorganic agent can handle other organic matters in soil and change the adsorption structure in soil and biology can
Usability is unfavorable for the growth of plant and microorganism, cannot effectively be used in combination with bioremediation technology.
For the efficiency for further increasing reparation, the engagement technique between chemical oxidation and biological treatment, this hair are solved the problems, such as
It is bright to propose a kind of joint processing technology based on the biological reinforced strategy of sodium peroxydisulfate oxidation processes and flora, to severe stone
Soil contaminated by crude oil or greasy filth are efficiently treated through.
Summary of the invention
It is biological reinforced based on the oxidation of sodium peroxydisulfate compound and microbial flora that the purpose of the present invention is to provide one kind
The method for closing processing soil with serious petroleum pollution or greasy filth, this method can improve soil with serious petroleum pollution or oil extremely significantly
The treatment effeciency of mud, and process cycle can be greatly shortened, combine sodium peroxydisulfate oxidation processes substrate it is selectively low, it is efficient,
Period is short and the biological reinforced processing of flora is mild, the environment amenable plurality of advantages of converted product, in contaminated soil or can contain
Greasy dirt sludge-polluted scene original position or dystopy processing soil with serious petroleum pollution or greasy filth.
Biological reinforced combination treatment method according to the present invention is using bacillus (Bacillus sp.) and branch top
Fungi-bacteria flora solid of spore mould (Acremonium sp.) or Phanerochaete chrysosporium (Phanerochaete sp.)
Microbial inoculum accesses in soil with serious petroleum pollution or greasy filth.
Bacterium according to the present invention is respectively bacillus, branch acremonium bacterium and Phanerochaete chrysosporium, in which: gemma bar
Bacterium Bacillus sp., bacterial strain deposit number CCTCC NO:AB 2014248, belongs to known solid fungicide, and the bacterium bacterium colony is in milky white
Color, bacterium colony is round, and surface is smooth, raised, neat in edge, and back side color is consistent with front, non-pigment distribution.Branch acremonium bacterium
Acremonium sp.Y0997, bacterial strain deposit number are CCTCC NO:M 2016117, and depositary institution is that Chinese Typical Representative culture is protected
Hiding center, address: Wuhan, China Wuhan University, postcode: 430072, preservation date is on March 14th, 2016, and the bacterium bacterium colony is in white
Color, bacterium colony is round, back side cream color to ochre yellow, hyphae colorless, and smooth and tool diaphragm, sporangiophore are long directly from mycelia
Out, tapered more Dan Sheng, conidium is colourless, smooth, forms tufted or chain, long spindle is pointed at both ends, has no thick wall spore
Son.Phanerochaete chrysosporium Phanerochaete sp.F0996, bacterial strain deposit number are CCTCC NO:M 2016116, preservation list
Position is China typical culture collection center, address: Wuhan, China Wuhan University, postcode: 430072, preservation date is 2016
March 14, the bacterium bacteria colony white, round, the back side is colourless, the only a small amount of protrusion of bacterium colony；Mycelia is colourless, fluffy flocculence, with
The extension of incubation time, villiform protrusion gradually flatten, mycelia disappear, formed aleuriospore.
To achieve the goals above, the technical solution adopted in the present invention comprises the steps of:
(1) it acquires soil with serious petroleum pollution or greasy filth is placed in processing pond, broken into pieces, mixed, or directly will pollution
The soil with serious petroleum pollution or greasy filth at scene are broken into pieces, are mixed；
(2) soil handled through step (1) is added in the ratio of 8~15g/kg using sodium peroxydisulfate compound as oxidant
Or in greasy filth, and the moisture content for adding water to soil or greasy filth mixes, oxidation processes 10~20 days up to 15~30%；
(3) cultured bacillus and branch top born of the same parents mould are respectively connected into step (2) treated soil or greasy filth
Mix bacterium agent or bacillus and Phanerochaete chrysosporium mix bacterium agent, bacillus and branch acremonium bacterium or yellow archespore
The inoculum concentration of the flat lead fungi of hair is 15~25g/kg, adds organic fertilizer, and the additive amount of organic fertilizer is 800~1200mg/
Kg is mixed, the surface of contaminated soil or greasy filth is covered with plastic film, is ventilated, and the moisture content of soil or greasy filth is maintained to stablize,
At 15~35 DEG C, in-situ processing 10~20 days；
(4) the cultured bacillus of equivalent and branch top born of the same parents are accessed again into step (3) treated soil or greasy filth
The mix bacterium agent of mould or the mix bacterium agent of bacillus and Phanerochaete chrysosporium repeat the operation of step (3), 15~
At 35 DEG C, in-situ processing 20~30 days, completion processing.
Above-mentioned sodium peroxydisulfate compound is accessed in the soil or greasy filth handled through step (1) in the ratio of 8~15g/kg, then
Biology or nontoxic surfactants are added, the additive amount of surfactant is 1200~1600mg/kg, and adds water to soil or oil
The moisture content of mud mixes, oxidation processes 10~20 days up to 15~30%；
Above-mentioned sodium peroxydisulfate compound is mixed by sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate with the molar ratio of 5:0.5:2.5
Conjunction is made.
The entitled Acremonium sp.Y0997 of above-mentioned branch acremonium bacterium, bacterial strain deposit number are CCTCC NO:M
2016117, the deposit date is on March 14th, 2016, and depositary institution is China typical culture collection center；Yellow archespore Mao Pingge
The entitled Phanerochaete sp.F0996 of bacterium, bacterial strain deposit number are CCTCC NO:M 2016116, during depositary institution is
State's Type Tissue Collection, preservation date are on March 14th, 2016.
The viable bacteria content of above-mentioned bacillus is 4.0 × 109~6.0 × 109The viable bacteria content of CFU/g, branch acremonium bacterium is
7.2×109~8.5 × 109CFU/g, the viable bacteria content of Phanerochaete chrysosporium are 7.2 × 109~9.0 × 109CFU/g。
The total petroleum hydrocarbon initial content of above-mentioned soil with serious petroleum pollution or greasy filth is 100000~170000mg/kg soil
Or greasy filth.
The present invention is based on the oxidations of sodium peroxydisulfate compound and the biological reinforced Combined Treatment serious petroleum pollution of microbial flora
The method of soil or greasy filth is first to connect twice in succession again after oxidant and surfactant cooperation carry out chemical oxidation treatment
The mixed bacterial for entering bacillus and branch acremonium bacterium or Phanerochaete chrysosporium carries out the biological reinforced reparation of sequence, by chemical oxygen
Change technology is capable of the degradation effect of the raising TPH on extremely significant ground in conjunction with the biological reinforced recovery technique of fungi-bacteria flora sequence
Rate, and substantially reduce repairing efficiency, combine that sodium peroxydisulfate oxidation processes substrate is selectively low, efficient, period is short and
Flora is biological reinforced to handle mild, the environment amenable plurality of advantages of converted product, can pollute in contaminated soil or oily sludge
Live original position or dystopy processing soil with serious petroleum pollution or greasy filth.Serious petroleum pollution to crude quality at concentrations up to 17%
The treatment effect of soil or greasy filth is obvious, and test result shows: after processing 10~20 days, TPH degradation efficiency in soil or greasy filth
It is 30%~50%, 35% or more has been reached to the degradation efficiency of wherein light chain and heavy chain component, respectively 38.53 ±
1.06%, 42.78 ± 1.45%, pass through subsequent bacillus sp.'s and branch acremonium bacterium Acremonium sp.
Flora is by a definite date after processing in 35~50 days, and TPH degradation efficiency has reached 86%~95% in soil or greasy filth, flora of the invention
Growth is stablized, and branch acremonium bacterium and Phanerochaete chrysosporium are preferable to the tolerance of outdoor elements at the scene, survival rate
It is higher, further it is proposed that technical system can apply to completely petroleum-type compound pollution a variety of media such as Polluted Soil
The production waste or petroleum wastewater etc. of earth or oily sludge and shale gas collection site, and had in oil producing operation area
On-site verification, it is reliable and stable, also can operate with petroleum-type compound pollution a variety of media such as contaminated soil or pollutant effluents with
And the production waste or petroleum wastewater etc. of shale gas collection site.
Detailed description of the invention
Fig. 1 is the bacterium colony figure of branch acremonium bacterium (Acremonium sp.Y0997).
Fig. 2 is the shape appearance figure of Phanerochaete chrysosporium (Phanerochaete sp.F0996).
Fig. 3 is the internal standard method for gas chromatography analysis map for handling petroleum the hydrocarbon component in 15 days blank example soil.
Fig. 4 is the internal standard method for gas chromatography analysis of petroleum the hydrocarbon component in 15 days 1 soil of embodiment of chemical oxidation treatment
Map.
Fig. 5 is the removal efficiency result of light chain and heavy chain component in 15 days 1 soil of embodiment of chemical oxidation treatment.
Fig. 6 is TPH removal efficiency comparing result in 60 days blank examples of processing and comparative example and 1 soil of embodiment.
Specific embodiment
Technical solution of the present invention is further described now in conjunction with test and embodiment, but the present invention is not limited only to
Embodiment disclosed below.
Embodiment 1
Now with bacillus, (Bacillus sp. is preserved in China typical culture collection center, bacterial strain deposit number
CCTCC NO:AB2014248) and branch acremonium bacterium (Acremonium sp.Y0997, is preserved in China typical culture collection
Center, bacterial strain deposit number be CCTCC NO:M 2016117, preservation date is on March 14th, 2016) solid fungicide be flora
Combination, using sodium peroxydisulfate compound as chemical oxidizing agent, combines and handles soil with serious petroleum pollution, by following steps group
At:
(1) soil with serious petroleum pollution that the total petroleum hydrocarbon initial content near oil well is 162240mg/kg is acquired to be placed in
It in processing pond, is broken into pieces, partial size is 30~40mm or so, is mixed；
(2) oxidant sodium peroxydisulfate compound is accessed in the soil handled through step (1) in the ratio of 10g/kg, adds water
To the moisture content of soil up to 25%, mix, oxidation processes 15 days, which was by sodium peroxydisulfate, calper calcium peroxide
It is mixed with ferrous sulfate with the molar ratio of 5:0.5:2.5；
(3) cultured bacillus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.) are accessed into weight
It spends in oil-polluted soils, the inoculum concentration of bacillus is 20g/kg soil (its solid fungicide viable bacteria content: 5.3 × 109CFU/
G), the inoculum concentration of branch acremonium bacterium is 20g/kg soil (its solid fungicide viable bacteria content: 8.0 × 109CFU/g), according still further to
Organic fertilizer is added in the amount of 1000mg/kg (soil), mixes, and maintains the moisture content of soil to stablize, the surface of processing pond is moulded
Expect that film covering, ventilation maintain the moisture content of soil to stablize, at 25 DEG C, in-situ processing 15 days；
(4) the cultured bacillus of equivalent and branch acremonium bacterium are accessed again into step (3) treated soil, weight
The operation of multiple step (3), at 25 DEG C, in-situ processing 30 days, completion processing, the degradation rate of TPH reached 86.96%.
The 18SrDNA sequence of above-mentioned branch acremonium bacterium (Acremonium sp.Y0997) are as follows:
The bacterium bacterium colony is white, and bacterium colony is round, back side cream color to ochre yellow, hyphae colorless, smooth and tool diaphragm, spore
Upright connect of capsule grows from mycelia, and tapered more Dan Sheng, conidium is colourless, smooth, formation tufted or chain, long spindle,
It is pointed at both ends, have no chlamydospore, pattern is referring to Fig. 1.
Embodiment 2
Now with Phanerochaete chrysosporium, (Phanerochaete sp.F0996, is preserved in China typical culture collection
The heart, bacterial strain deposit number CCTCC NO:M 2016116, preservation date are on March 14th, 2016) solid fungicide be flora composition,
Using sodium peroxydisulfate compound as chemical oxidizing agent, combine and soil with serious petroleum pollution handled, comprises the steps of:
(1) soil with serious petroleum pollution that the total petroleum hydrocarbon initial content near oil well is 170000mg/kg is acquired to be placed in
It in processing pond, is broken into pieces, partial size is 30~40mm or so, is mixed；
(2) oxidant sodium peroxydisulfate compound is accessed in the soil handled through step (1) in the ratio of 8g/kg, adds water
To the moisture content of soil up to 15%, mix, oxidation processes 20 days, sodium peroxydisulfate compound used was by sodium peroxydisulfate, peroxidating
Calcium and ferrous sulfate are mixed with the molar ratio of 5:0.5:2.5；
(3) by cultured bacillus (Bacillus sp.) and Phanerochaete chrysosporium (Phanerochaete
Sp.F0996 it) accesses in soil with serious petroleum pollution, the inoculum concentration of bacillus is that (its solid fungicide viable bacteria contains 15g/kg soil
Amount: 4.0 × 109CFU/g), the inoculum concentration of Phanerochaete chrysosporium be 15g/kg soil (its solid fungicide viable bacteria content: 7.5 ×
109CFU/g), organic fertilizer is added according still further to the amount of 800mg/kg (soil), mixes, maintain the moisture content of soil to stablize, will locate
The surface in reason pond is covered with plastic film, is ventilated, and the moisture content of soil is maintained to stablize, at 15 DEG C, in-situ processing 20 days；
(4) the cultured bacillus of equivalent and yellow archespore Mao Pingge are accessed again into step (3) treated soil
The mix bacterium agent of bacterium repeats the operation of step (3), and at 15 DEG C, in-situ processing 30 days, completion processing, the degradation rate of TPH reached
85.25%.
Above-mentioned Phanerochaete chrysosporium Phanerochaete sp.F0996, bacterial strain deposit number are CCTCC NO:M
2016116, depositary institution is China typical culture collection center, and preservation date is on March 14th, 2016,18SrDNA sequence
It is classified as:
The bacterium bacteria colony white, round, the back side is colourless, the only a small amount of protrusion of bacterium colony；Mycelia is colourless, fluffy flocculence, with
The extension of incubation time, villiform protrusion gradually flatten, mycelia disappear, formed aleuriospore, referring to fig. 2.
Embodiment 3
Using sodium peroxydisulfate compound as chemical oxidizing agent, combine bacillus and branch acremonium bacterium mixed bacterial to severe stone
Oily pollution greasy filth carries out reinforcing Combined Treatment, comprises the steps of:
(1) the serious petroleum pollution greasy filth that the total petroleum hydrocarbon initial content near oil well is 100000mg/kg is acquired to be placed in
It in processing pond, is broken into pieces, partial size is 30~40mm or so, is mixed；
(2) oxidant sodium peroxydisulfate compound is accessed in the greasy filth handled through step (1) in the ratio of 12g/kg, over cure
Sour sodium compound is mixed by sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate with the molar ratio of 5:0.5:2.5, according still further to
Heavy oil cleaning agent KD-L315 is added as surfactant in the additive amount of 1200mg/kg (greasy filth), and adds water to the aqueous of greasy filth
Rate mixes, oxidation processes 10 days up to 30%；
(3) cultured bacillus (Bacillus sp.) and branch acremonium bacterium (Acremonium sp.) are accessed into weight
It spends in oil pollution greasy filth, the inoculum concentration of bacillus is 25g/kg greasy filth (its solid fungicide viable bacteria content: 6.0 × 109CFU/
G), the inoculum concentration of branch acremonium bacterium is 25g/kg greasy filth (its solid fungicide viable bacteria content: 8.5 × 109CFU/g), according still further to
Organic fertilizer is added in the amount of 1200mg/kg (greasy filth), mixes, and maintains the moisture content of greasy filth to stablize, the surface of processing pond is moulded
Expect that film covering, ventilation maintain the moisture content of greasy filth to stablize, at 35 DEG C, in-situ processing 10 days；
(4) the cultured bacillus of equivalent and branch acremonium bacterium are accessed again into step (3) treated greasy filth, weight
The operation of multiple step (3), at 35 DEG C, in-situ processing 20 days, completion processing, the degradation rate of TPH reached 95.01%.
Embodiment 4
Using sodium peroxydisulfate compound as chemical oxidizing agent, combine bacillus and Phanerochaete chrysosporium mixed bacterial counterweight
Degree oil pollution greasy filth carries out reinforcing Combined Treatment, comprises the steps of:
(1) the serious petroleum pollution greasy filth that the total petroleum hydrocarbon initial content near oil well is 120000mg/kg is acquired to be placed in
It in processing pond, is broken into pieces, partial size is 30~40mm or so, is mixed；
(2) oxidant sodium peroxydisulfate compound is accessed in the greasy filth handled through step (1) in the ratio of 15g/kg, over cure
Sour sodium compound is mixed by sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate with the molar ratio of 5:0.5:2.5, according still further to
The additive amount of 1600mg/kg (greasy filth) be added heavy oil cleaning agent KD-L315 as surfactant or other it is commercially available biological or
Nontoxic surfactants, and the moisture content for adding water to greasy filth mixes, oxidation processes 10 days up to 30%；
(3) by cultured bacillus (Bacillus sp.) and Phanerochaete chrysosporium (Phanerochaete
Sp. in mix bacterium agent access serious petroleum pollution greasy filth), the inoculum concentration of bacillus is 25g/kg greasy filth (its solid fungicide
Viable bacteria content: 5.0 × 109CFU/g), the inoculum concentration of Phanerochaete chrysosporium is that (its solid fungicide viable bacteria contains 25g/kg greasy filth
Amount: 9.0 × 109CFU/g), organic fertilizer is added according still further to the amount of 1200mg/kg (greasy filth), mixes, maintains the moisture content of greasy filth
Stablize, the surface of processing pond is covered with plastic film, ventilate, the moisture content of greasy filth is maintained to stablize, at 35 DEG C, in-situ processing
10 days；
(4) the cultured bacillus of equivalent and yellow archespore Mao Pingge are accessed again into step (3) treated greasy filth
Bacterium repeats the operation of step (3), and at 35 DEG C, in-situ processing 20 days, completion processing, the degradation rate of TPH reached 93.31%.
Method of the invention is applicable to processing soil with serious petroleum pollution and greasy filth, and it is existing can also directly to handle pollution
The soil with serious petroleum pollution or greasy filth of field, can obtain good treatment effect.
Above-mentioned branch top born of the same parents mould can be replaced mutually with Phanerochaete chrysosporium, be mixed to form very with bacillus
Bacterium-bacterium mix bacterium agent has a preferable effect to above-mentioned soil with serious petroleum pollution and greasy filth, especially with oxidant oxygen
Change Combined Treatment.
In order to verify technical effect of the invention, applicant has done a large amount of experiment, while also using blank assay as ginseng
According to, degradation effect of the invention is verified, specific as follows:
Comparative example
(1) total petroleum hydrocarbon initial content is that 162240mg/kg soil with serious petroleum pollution is placed in processing pond, is struck
It is broken, it mixes；
(2) identical as (3) the step of embodiment 1.
(3) identical as (4) the step of embodiment 1.
Blank example:
(1) identical as comparative example 1；
(2) organic fertilizer and surface-active is added into the contaminated soil that total petroleum hydrocarbon initial content is 162240mg/kg
Agent, and the moisture content for adding water to soil is mixed up to 25%, at 15~35 DEG C, in-situ processing 60 days, completion processing.
Total petroleum hydrocarbon component carries out gas-chromatography and mass spectrum in soil after handling 15 days above-mentioned blank example and embodiment 1
Combination detection, as a result shown in following Fig. 3 and Fig. 4.
By in Fig. 3 and 4 the results show that compared with blank example, it is each in soil after sodium peroxydisulfate compound oxidation processes
Petroleum the hydrocarbon component has obvious degradation effect, and carries out to the removal efficiency of light chain and heavy chain component in soil after oxidation processes
It calculates, as a result as shown in Figure 5.As shown, light chain and heavy chain component after sodium peroxydisulfate compound oxidation processes, in soil
Removal efficiency respectively reached 38.53 ± 1.06%, 42.78 ± 1.45%.
The degradation situation of total petroleum hydrocarbon in soil after handled by above-mentioned comparative example and blank example is detected, and with reality
It applies example 1 to be compared, as a result as shown in the following table 1 and Fig. 6.
Table 1 is the comparing result of TPH content and embodiment 1 in comparative example and blank example treated soil
By table 1 and Fig. 6 it is found that comparative example and blank example handle 60 days after soil in TPH content and embodiment 1 comparison
The result shows that the TPH removal efficiency that improves in soil extremely significant using the embodiment 1 of chemical oxidation pre-treatment, with comparison
Example is compared, and the TPH removal efficiency of embodiment 1 improves about 22.54%.
It is also handled with greasy filth of the identical method to serious petroleum pollution, can also reach above-mentioned identical processing effect
Fruit.
Test result shows the present invention using chemical oxidation and the biological reinforced comprehensive in-situ processing weight of fungi-bacteria flora
Oil-polluted soils or greasy filth are spent, the degradation efficiency of total petroleum hydrocarbon in soil or greasy filth can be improved extremely significantly.
Claims (2)
1. one kind is based on the oxidation of sodium peroxydisulfate compound and the biological reinforced Combined Treatment soil with serious petroleum pollution of microbial flora
Or the method for greasy filth, it is characterised in that comprise the steps of:
(1) it acquires soil with serious petroleum pollution or greasy filth is placed in processing pond, broken into pieces, mixed, or directly will pollution scene
Soil with serious petroleum pollution or greasy filth break into pieces, mix；
(2) soil or oil handled through step (1) is added in the ratio of 8 ~ 15 g/kg using sodium peroxydisulfate compound as oxidant
In mud, surfactant is added, the additive amount of surfactant is 1200 ~ 1600 mg/kg, and adds water to soil or greasy filth
Moisture content up to 15 ~ 30%, mix, oxidation processes 10 ~ 20 days；
Above-mentioned sodium peroxydisulfate compound is mixed by sodium peroxydisulfate, calper calcium peroxide and ferrous sulfate with the molar ratio of 5:0.5:2.5
It is made；
(3) be respectively connected into step (2) treated soil or greasy filth cultured bacillus (BacillusSp.) with
Branch top born of the same parents mould (AcremoniumSp. mix bacterium agent or bacillus and Phanerochaete chrysosporium)
(PhanerochaeteSp. the inoculum concentration of mix bacterium agent), bacillus and branch acremonium bacterium or Phanerochaete chrysosporium is equal
For 15 ~ 25 g/kg, organic fertilizer is added, the additive amount of organic fertilizer is 800 ~ 1200 mg/kg, is mixed, by contaminated soil
Or the surface of greasy filth is covered with plastic film, ventilation, and the moisture content of soil or greasy filth is maintained to stablize, at 15 ~ 35 DEG C, at scene
Reason 10 ~ 20 days；
(4) the cultured bacillus of equivalent and branch top born of the same parents mould are accessed again into step (3) treated soil or greasy filth
Mix bacterium agent or bacillus and Phanerochaete chrysosporium mix bacterium agent, repeat step (3) operation, at 15 ~ 35 DEG C
Under, in-situ processing 20 ~ 30 days, completion processing；
Above-mentioned Phanerochaete chrysosporium (PhanerochaeteSp. entitled F0996), in preservation on March 14 in 2016
In China typical culture collection center, deposit number is CCTCC NO:M 2016116, and preservation address is that Wuhan City, Hubei Province is military
Chinese university；Branch acremonium bacterium (AcremoniumSp. entitled Y0997) is preserved in Chinese Typical Representative on March 14th, 2016
Culture collection, deposit number are CCTCC NO:M 2016117, and preservation address is Wuhan City, Hubei Province Wuhan University；Bud
Spore bacillus (BacillusSp. deposit number) is CCTCC NO: AB 2014248.
2. according to claim 1 based on the oxidation of sodium peroxydisulfate compound and the biological reinforced Combined Treatment weight of microbial flora
Spend the method for oil-polluted soils or greasy filth, it is characterised in that: the viable bacteria content of the bacillus is 4.0 × 109~ 6.0×
109CFU/g, the viable bacteria content of branch acremonium bacterium are 7.2 × 109 ~8.5×109The viable bacteria of CFU/g, Phanerochaete chrysosporium contain
Amount is 7.2 × 109 ~9.0×109 CFU/g。
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