CN112451438B - Ginseng ferment and polypeptide composition - Google Patents

Ginseng ferment and polypeptide composition Download PDF

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CN112451438B
CN112451438B CN202011412228.3A CN202011412228A CN112451438B CN 112451438 B CN112451438 B CN 112451438B CN 202011412228 A CN202011412228 A CN 202011412228A CN 112451438 B CN112451438 B CN 112451438B
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ginseng
ferment
polypeptide
ginseng ferment
composition
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CN112451438A (en
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侯剑峰
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Shiqi Biological Research And Development Center Suzhou Industrial Park Co ltd
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Shiqi Biological Research And Development Center Suzhou Industrial Park Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/63—Steroids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Dermatology (AREA)
  • Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Emergency Medicine (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The invention relates to the field of cosmetics, and particularly discloses a ginseng ferment and polypeptide composition; aiming at solving the problem that the application cost is very high because the addition amount of ginseng ferment which is separately applied to the anti-aging cosmetics needs to be high to achieve a good effect in the prior art; the ginseng ferment disclosed by the invention is prepared from a ginseng product by fermenting with lactic acid bacteria. The ginseng ferment contains saponins such as CK, Rb1, Rb2, Rg3 and the like through detection, and has the protection in the aspects of photo-aging resistance and the like. The application examples of the invention include but are not limited to the following components: disodium ethylene diamine tetraacetate, carbomer, butanediol, glycerol, aminomethyl propanol, betaine, rhamnose, ginseng ferment, a polypeptide compound containing palmitoyl oligopeptide, PEG-40 hydrogenated castor oil, essence and the balance of water. Meanwhile, the ginseng ferment also contains organic acid such as lactic acid and the like, so that cutin peeling is promoted. The ginseng ferment contains micromolecular ginseng polysaccharide and ginseng polypeptide and has good nourishing effect.

Description

Ginseng ferment and polypeptide composition
Technical Field
The invention relates to the field of cosmetics, and particularly relates to a ginseng ferment and polypeptide composition.
Background
Ginseng has many efficacies in cosmetics, and its anti-aging function is widely known. The ginseng has good effects on resisting natural aging, resisting photoaging and resisting gene aging.
Resisting natural aging: increase human fibroblast proliferation, reduce MMP-1 (matrix metalloproteinase-1), and improve collagen metabolism.
The anti-photoaging aspect is divided into two aspects, anti-keratinocyte photoaging and anti-fibroblast photoaging. Anti-keratinocyte photoaging: the monomeric saponins Rb1 and CK can promote DNA photodamage repair. R1, Rg1, CK and Rg3 can inhibit keratinocyte apoptosis caused by ultraviolet rays. Rb1, Rb2, Rb3 and Rg3 can improve the activities of antioxidase such as SOD (superoxide dismutase) and glutathione irradiated by ultraviolet rays, reduce the content of ROS (oxygen free radical), reduce the expression of MMP-2 (matrix metalloproteinase-2) and resist photoaging.
Anti-fibroblast photoaging: rb1, Rb2, Rg1 reduce oxidative stress levels in fibroblasts caused by UVB (ultraviolet B band). Rb1, Rb2, RD, Rg1, CK and the like reduce the expression or activity of collagen hydrolase MMP-1, MMP-2 and MMP-3 in fibroblasts, and delay the degradation of collagen to reach the photoaging effect of the dermis. In the aspect of gene anti-aging: the ginsenoside Rg1 can exert its antiaging effect by changing the expression of cell cycle regulatory factor.
However, the addition amount of the antioxidant is high to achieve a good effect when the antioxidant is independently applied to the anti-aging cosmetic, so that the application cost is high.
Peptide substances and hydrolyzed collagen have good anti-aging effect and are widely applied to cosmetics at present, but the price of raw materials is relatively high, and the effect is inferior to that of the peptide substances and the hydrolyzed collagen when the peptide substances and the hydrolyzed collagen are compounded with other anti-aging ingredients.
Disclosure of Invention
The invention provides a composition of ginseng ferment and polypeptide, and solves the problem that in the prior art, the application cost is high due to the fact that the addition amount of ginseng ferment which is independently applied to an anti-aging cosmetic needs to be high to achieve a good effect.
The technical scheme of the invention is realized as follows: a composition of ginseng ferment and polypeptide comprises a ginseng ferment and polypeptide composition in a ratio of 1:1-10: 1.
Further, the ratio of the ginseng ferment to the polypeptide composition is 2: 1.
Further, the ginseng ferment is prepared by fermenting a ginseng product through lactic acid bacteria, and the ginseng ferment also contains organic acid, micromolecular ginseng polysaccharide, ginseng polypeptide and saponin.
Further, the organic acid in the ginseng ferment is lactic acid.
Further, the composition comprises, by weight, 0.02-0.1% of disodium edetate, 0.1-0.5% of carbomer, 2-10% of butanediol, 0.5-10.0% of glycerol, 0.1-0.3% of aminomethyl propanol, 0.1-5% of betaine, 0.2-51.0% of rhamnose, 1.0-50.0% of ginseng ferment, 0.5-25% of polypeptide complex containing palmitoyl oligopeptide, 0.1-0.6% of PEG-40 hydrogenated castor oil, 0.05-0.2% of essence, and the balance of water.
The ginseng ferment and polypeptide composition disclosed by the invention has the following beneficial effects:
1. the ginseng ferment disclosed by the invention is prepared from a ginseng product by fermenting with lactic acid bacteria. The ginseng ferment contains saponins such as CK, Rb1, Rb2, Rg3 and the like through detection, and has the protection in the aspects of photo-aging resistance and the like. Meanwhile, the ginseng ferment also contains organic acid such as lactic acid and the like, so that cutin peeling is promoted. The ginseng ferment contains micromolecular ginseng polysaccharide and ginseng polypeptide and has good nourishing effect.
2. The palmitoyl oligopeptide has the effects of promoting the synthesis of tissue growth factor TGF-beta, improving the activity of cells and promoting the synthesis of collagen. Hydrolyzed collagen is a major component of the dermal extracellular matrix, is a structural protein, and provides a raw material for collagen synthesis.
The ginseng ferment is prepared from a ginseng product by lactobacillus fermentation. The ginseng ferment contains saponins such as CK, Rb1, Rb2, Rg3 and the like through detection, and has the effects of resisting photoaging, repairing DNA damage, resisting oxidation and the like. Meanwhile, the ginseng ferment also contains organic acid such as lactic acid and the like, so that cutin peeling is promoted. The ginseng ferment contains micromolecular ginseng polysaccharide and ginseng polypeptide and has good nourishing effect.
Detailed Description
The technical solutions in the present invention will be described clearly and completely below with reference to embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Examples
Components Example 1 Example 2 Example 3 Example 4 Example 5
Polypeptide complexes 0.1％ - - - -
Ginseng ferment - 0.025％ 0.05％ 0.1％ 0.2％
Components Example 6 Example 7 Example 8 Example 9 Comparative example 1
Polypeptide complexes 0.1％ 0.1％ 0.1％ 0.1％ -
Ginseng ferment 0.025％ 0.05％ 0.1％ 0.2％ -
Examples of the experiments
The present invention analyzes the effectiveness of each example and comparative example by determining the sum of the keratinocyte viability and the fibroblast viability.
1. Keratinocyte proliferation promoting model
The test method comprises the following steps: taking logarithmic phase growing keratinocyte (HaCaT), at 1 × 105/ml
Densities were seeded in 96-well plates, respectively. After 24h, the keratinocytes were treated with 7 samples of ginseng ferment or/and polypeptide complex formulated at different concentrations and incubated for 72h in a carbon dioxide incubator. Cell viability was measured by MTT method and cell viability was calculated.
Blank control group and experimental group detection data are subjected to blank value deduction and analysis is carried out. Plotting was performed using GraphPad software.
Cell Viability% (% ViabilityI) experimental group/blank control group × 100%
Serial number Sample (I) Cell viability/W/W%
1 Comparative example 100.00
2 Experimental example 1 124.29
3 Experimental example 2 105.17
4 Experimental example 3 106.73
5 Experimental example 4 122.13
6 Experimental example 5 86.67
7 Experimental example 6 118.24
8 Experimental example 7 132.80
9 Experimental example 8 145.20
10 Experimental example 9 152.74
Table 1: keratinocyte proliferation data
From the results analysis, it was found that the effect of ginseng ferment on the cell viability was limited when it was applied alone to keratinocytes, but when it was applied together with the polypeptide complex, the activity-promoting effect on the keratinocytes was significantly increased, and a positive correlation was observed with the increase in the ratio of ginseng ferment added.
2. Model for promoting proliferation of fiber cells
The test method comprises the following steps: human primary fibroblasts grown in the logarithmic phase were seeded in 96-well plates at a density of 5X 104 cells/ml, respectively. After 24h, fibroblasts were treated with samples of ginseng ferment or/and polypeptide complex formulated to different concentrations and incubated in a carbon dioxide incubator for 72 h. Cell viability was measured by MTT (MTT is a yellow compound) colorimetry, and cell viability was calculated.
Blank control group (CK group) and experimental group detection data are subjected to analysis by deducting blank value. Plotting was performed using GraphPad software.
Cell Viability% (% ViabilityI) experimental group/blank control group × 100%
And (3) modeling results:
serial number Sample (I) Cell viability/%
1 Comparative example 100.00
2 Experimental example 1 144.94
3 Experimental example 2 90.37
4 Experimental example 3 92.44
5 Experimental example 4 109.07
6 Experimental example 5 124.74
7 Experimental example 6 141.12
8 Experimental example 7 152.84
9 Experimental example 8 153.37
10 Experimental example 9 180.18
Table 2: fibroblast proliferation data
From the results analysis, it is found that the effect of ginseng ferment on the cell viability is limited when the ginseng ferment is used alone on fibroblasts, but when the ginseng ferment is used together with the polypeptide complex, the promotion effect on the cell viability is obviously increased, and the positive correlation is shown along with the increase of the addition ratio of the ginseng ferment.
Based on the data, the ginseng ferment and the polypeptide compound both exert a synergistic effect on proliferation models of keratinocytes and fibroblasts, and the proliferation promoting effect of the two compound preparations is stronger than that of the ginseng ferment or the polypeptide compound under a single concentration. Preferably, the ginseng ferment with 0.2% concentration is compounded with the 0.1% polypeptide complex to be tested in parallel, and the average value + -standard deviation (Mean + -SD) is analyzed, and compared with the blank group (CK group), the P value of the result obtained by 0.1% polypeptide complex plus 0.2% ginseng ferment is less than 0.001.
Figure 1
Table 3: keratinocyte proliferation data
Figure 2
Table 4: fibroblast proliferation data
Further application examples contain, but are not limited to, the following components:
0.02-0.1% of disodium ethylene diamine tetraacetate, 0.1-0.5% of carbomer, 2-10% of butanediol, 0.5-10.0% of glycerol, 0.1-0.3% of aminomethyl propanol, 0.1-5% of betaine, 0.2-51.0% of rhamnose, 1.0-50.0% of ginseng ferment, 0.5-25% of polypeptide compound containing palmitoyl oligopeptide, 0.1-0.6% of PEG-40 hydrogenated castor oil and 0.05-0.2% of essence, and the balance of water.
3. Skin elasticity repair experiment
Example 10
Figure BDA0002816926800000063
Figure BDA0002816926800000071
1% of polypeptide complex containing palmitoyl oligopeptide,
PEG-40 hydrogenated Castor oil 0.3%
0.1 percent of essence,
the balance being water.
This example selects 30 women with facial skin showing loose elasticity in asian between 25 and 45 years old, the face is used once a day, morning and night, for a total of 14 days, and the skin elasticity is measured by a skin elasticity meter Cutometer MPA580, which measures the skin elasticity before use D0, for 14 days.
D0 D14
R2 value 0.609 0.665
TABLE 5 skin elasticity R2 values
The measured value R2 was approximately 1, the better the skin elasticity. The skin elasticity increased by 9.04% with 14 days.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (4)

1. The composition of ginseng ferment and polypeptide is characterized by consisting of the ginseng ferment and the polypeptide composition, wherein the ratio of the ginseng ferment to the polypeptide composition is 2:1, and the polypeptide composition consists of palmitoyl oligopeptide and hydrolyzed collagen.
2. The composition of claim 1, wherein the ginseng ferment is prepared from a ginseng product by lactobacillus fermentation, and the ginseng ferment further comprises an organic acid, a small-molecule ginseng polysaccharide, ginseng polypeptide and saponin.
3. The composition of claim 2, wherein the organic acid in the ginseng ferment is lactic acid.
4. A composition of ginseng ferment and polypeptide is characterized by comprising the following components, by weight, 0.02-0.1% of disodium ethylene diamine tetraacetate, 0.1-0.5% of carbomer, 2-10% of butanediol, 0.5-10.0% of glycerol, 0.1-0.3% of aminomethyl propanol, 0.1-5% of betaine, 0.2-51.0% of rhamnose, 1.0-50.0% of ginseng ferment, 0.5-25% of polypeptide composition, 0.1-0.6% of PEG-40 hydrogenated castor oil, 0.05-0.2% of essence and the balance of water; wherein the ratio of the ginseng ferment to the polypeptide composition is 2:1, and the polypeptide composition consists of palmitoyl oligopeptide and hydrolyzed collagen.
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丁家宜肌源焕活紧致面霜;丁家宜（苏州工业园区）化妆品贸易有限公司;《国产非特殊用途化妆品备案平台》;20201116;第1-2页 *

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