CN112430162A - Lactobacillus and application thereof in bio-organic fertilizer - Google Patents

Lactobacillus and application thereof in bio-organic fertilizer Download PDF

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Publication number
CN112430162A
CN112430162A CN202011466151.8A CN202011466151A CN112430162A CN 112430162 A CN112430162 A CN 112430162A CN 202011466151 A CN202011466151 A CN 202011466151A CN 112430162 A CN112430162 A CN 112430162A
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China
Prior art keywords
parts
lactobacillus
organic fertilizer
polyacrylamide
bio
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CN202011466151.8A
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Chinese (zh)
Inventor
姜广品
黄超
赵宪琳
孙燕玲
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Ji'nan Daxin Biological Science And Technology Co ltd
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Ji'nan Daxin Biological Science And Technology Co ltd
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Priority to CN202011466151.8A priority Critical patent/CN112430162A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/50Surfactants; Emulsifiers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/10Solid or semi-solid fertilisers, e.g. powders

Abstract

The invention belongs to the field of microbial fertilizers, and particularly relates to lactobacillus and application thereof to a biological organic fertilizer. The lactobacillus isLactobacilus sp.Has been delivered to the China general microbiological culture Collection center for preservation in 2020, 11/2, month, with the preservation numbers: CGMCC NO. 20994. The bio-organic fertilizer comprises the following raw materials in parts by weight: 60-100 parts of livestock manure, 10-18 parts of polyacrylamide, 60-100 parts of corncob powder and lactobacillusLactobacilus sp.3-8 parts of microbial inoculum, 0.2-0.7 part of alginate and K2HPO41-5 parts of ferric ammonium citrate, 0.5-1 part of ferric ammonium citrate and 0.5-1 part of fatty alcohol. The nutrient components are continuously provided for the growth of crops, and the nutrition does not need to be supplemented in the growth period of greenhouse crops; the yield, the soluble solid content and the taste of the strawberry crops are improved.

Description

Lactobacillus and application thereof in bio-organic fertilizer
Technical Field
The invention belongs to the field of microbial fertilizers, and particularly relates to lactobacillus and application thereof to a biological organic fertilizer.
Background
The fertilizer is grain of crops, and the reasonable application of the fertilizer is one of important factors for ensuring the high and stable yield of the crops and promoting the full development of agriculture in China. The fertilizer is mainly divided into inorganic fertilizer and organic fertilizer, wherein the inorganic fertilizer is also called chemical fertilizer, such as nitrogenous fertilizer, phosphate fertilizer, potash fertilizer and the like. In modern agriculture, as a large amount of chemical fertilizers and pesticides are used in pursuit of agricultural high yield, the chemicals are applied to soil, so that the physical and chemical properties and biochemical properties of the soil are rapidly deteriorated, soil hardening and hardening are realized, microorganisms are eliminated, the loss of organic matters is serious, the soil fertility is reduced, the fertilization effect is poor, crop diseases are frequent, and the original soil loses natural ecological balance. The organic fertilizer can effectively improve soil nutrients, increase soil organic matters and fertility, reduce pesticide application, prevent agricultural products from being polluted, optimize soil ecological environment, and improve the quality and taste of the agricultural products to return to nature; the organic fertilizer can improve the crop yield and the crop quality, contains rich organic matters and comprehensive nutrient elements, and improves the green and environment-friendly nutrients for crops.
Disclosure of Invention
Aiming at the problem of less cultivated land per capita in China, and combining the improvement effect of the organic fertilizer, the invention provides the lactobacillus, the biological organic fertilizer is prepared by adopting the lactobacillus, and the purposes of providing nutrition for the growth of crops in different periods, improving the yield, finally optimizing the soil ecological environment and improving the taste of agricultural products to return to nature are realized through the action and the layered structure of the lactobacillus. In addition, the invention also provides a preparation method and application of the biological organic fertilizer.
The technical scheme of the invention is as follows:
lactobacillusLactobacilus sp.The strain is delivered to the China general microbiological culture Collection center for preservation in 11/2/2020, and the preservation number is as follows: CGMCC NO.20994, with the preservation address of No. 3 Hospital No. 1 Xilu on Beijing, Chaoyang.
The application of the lactobacillus in the bio-organic fertilizer.
Comprises the lactobacillusLactobacilus sp.The biological organic fertilizer comprises the following raw materials in parts by weight: 60-100 parts of livestock manure, 10-18 parts of polyacrylamide, 60-100 parts of corncob powder and lactobacillusLactobacilus sp.3-8 parts of microbial inoculum, 0.2-0.7 part of alginate and K2HPO41-5 parts of0.5-1 part of ferric ammonium citrate and 0.5-1 part of fatty alcohol.
The preparation method of the biological organic fertilizer comprises the following steps:
(1) alginate, K2HPO4Mixing ferric ammonium citrate and fatty alcohol, adding a small amount of water to form granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding part of polyacrylamide, and forming;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
Preferably, the polyacrylamide added in the step (3) accounts for 1/3-1/2 in total.
The biological organic fertilizer prepared by the method is applied to greenhouse crop planting.
Preferably, the method is applied to strawberry planting.
The corncob meal can be adsorbed and stored under the condition of sufficient water and nutrient components, and can supply water and nutrients to crops under the condition of insufficient water and nutrient components, thereby playing the role of slow release of fertilizer efficiency. The corn cob meal fatty alcohol (hydroxyl and surfactant) has a synergistic effect, promotes the hydrolysis of ferric ammonium citrate and alginate, and provides nutrient components for the growth of crops continuously.
The invention has the advantages of
1. By means of the layered structure, the crop can be nourished in different periods. The polyacrylamide has strong flocculation effect and can be adsorbed and bridged among tiny particles; meanwhile, polar group particles on the polyacrylamide can also adsorb a plurality of ions, so that the fertilizer retention of the soil is enhanced. The fermented livestock and poultry manure is rich in nutrition and provides nutrition for the budding period; the corn cob powder and the fatty alcohol (hydroxyl and surfactant) generate a synergistic effect, the hydrolysis of the ferric ammonium citrate and the alginate is promoted, the nutrient components are continuously provided for the growth of crops, and the nutrients do not need to be supplemented in the growth period of the peanuts.
2. The corncob powder is favorable for air circulation, increases the water holding capacity of soil, and provides a good living space for animals and microorganisms with different sizes and body types and aerobic and anaerobic life habits, so that the corncob powder is favorable for water retention, fertilizer retention, ventilation and promotion of plant root system development, improves the utilization rate of soil moisture, and provides a comfortable growing environment for crops in saline-alkali soil.
3. The lactobacillus acts on corn cob powder, livestock manure and other raw materials, degrades the raw materials, changes the nutrient components of the existing organic fertilizer, is easy to be absorbed by crops, and improves the yield of strawberry crops, the content of soluble solid matters and the taste.
Detailed Description
Example 1
Lactobacillus-containing foodLactobacilus sp.The biological organic fertilizer comprises the following raw materials in parts by weight: 600g of livestock manure, 100g of polyacrylamide, 600g of corncob powder and lactobacillusLactobacilus sp.30g of microbial inoculum, 2g of alginate and K2HPO4 10g, 5g ferric ammonium citrate and 5g fatty alcohol.
The preparation method of the biological organic fertilizer comprises the following steps:
(1) alginate, K2HPO4Mixing ferric ammonium citrate and fatty alcohol, adding a small amount of water to form granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding 1/3 polyacrylamide, and molding;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
Example 2
Lactobacillus-containing foodLactobacilus sp.The biological organic fertilizer comprises the following raw materials in parts by weight: 1000g of livestock and poultry manure, 180g of polyacrylamide, 1000g of corncob powder and lactobacillusLactobacilus sp.0g of microbial inoculum, 7g of alginate and K2HPO450g, 10g ferric ammonium citrate and 10g fatty alcohol.
The preparation method of the biological organic fertilizer comprises the following steps:
(1) alginate, K2HPO4Mixing ferric ammonium citrate and fatty alcohol, adding a small amount of water to form granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding 1/2 polyacrylamide, and molding;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
Example 3
Lactobacillus-containing foodLactobacilus sp.The biological organic fertilizer comprises the following raw materials in parts by weight: 800g of livestock manure, 150g of polyacrylamide, 800g of corncob powder and lactobacillusLactobacilus sp.60g of microbial inoculum, 5g of alginate and K2HPO430g, 8g ferric ammonium citrate and 7g fatty alcohol.
The preparation method of the biological organic fertilizer comprises the following steps:
(1) alginate, K2HPO4Mixing ferric ammonium citrate and fatty alcohol, adding a small amount of water to form granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding 1/3 polyacrylamide, and molding;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
Comparative example 1
Lactobacillus-containing foodLactobacilus sp.The biological organic fertilizer comprises the following raw materials in parts by weight: 800g of livestock and poultry manure, 150g of polyacrylamide, 800g of corncob powder, 5g of alginate and K2HPO430g, 8g ferric ammonium citrate and 7g fatty alcohol.
The preparation method of the biological organic fertilizer comprises the following steps:
(1) alginate, K2HPO4Ammonium ferric citrate, fatty alcohol, mixing and addingA small amount of water forms granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding 1/3 polyacrylamide, and molding;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
Examples of the effects of the invention
Firstly, the biological organic fertilizer prepared according to the examples 1-3 and the comparative example 1 is fertilized according to 60 kg/mu, 3 mu of fertilizer is selected for each group, and the effect of the biological organic fertilizer applied to the strawberry planting in a certain test greenhouse base in Shandong province is studied as follows:
Figure DEST_PATH_IMAGE002

Claims (7)

1. lactobacillusLactobacilus sp.The strain is delivered to the China general microbiological culture Collection center for preservation in 11/2/2020, and the preservation number is as follows: CGMCC NO.20994, with the preservation address of No. 3 Hospital No. 1 Xilu on Beijing, Chaoyang.
2. Use of the lactobacillus of claim 1 in bio-organic fertilizers.
3. A method for producing a Lactobacillus strain comprising the Lactobacillus strain of claim 1Lactobacilus sp.The biological organic fertilizer is characterized by comprising the following raw materials in parts by weight: 60-100 parts of livestock manure, 10-18 parts of polyacrylamide, 60-100 parts of corncob powder and lactobacillusLactobacilus sp.3-8 parts of microbial inoculum, 0.2-0.7 part of alginate and K2HPO41-5 parts of ferric ammonium citrate, 0.5-1 part of ferric ammonium citrate and 0.5-1 part of fatty alcohol.
4. A preparation method of the bio-organic fertilizer as claimed in claim 3, characterized by comprising the following steps:
(1) alginate, K2HPO4Mixing ferric ammonium citrate and fatty alcohol, adding a small amount of water to form granules;
(2) mixing the corn cob powder, the livestock manure and the lactobacillus microbial inoculum;
(3) adding the particle type obtained in the step (1) into the step (2), adding part of polyacrylamide, and forming;
(4) adding the particles formed in the step (3) into the residual polyacrylamide, and pressing and forming; the product is obtained.
5. The method for preparing the bio-organic fertilizer as claimed in claim 4, wherein the polyacrylamide added in the step (3) accounts for 1/3-1/2 in total.
6. The use of a bio-organic fertilizer prepared by the method of claim 4 for greenhouse crop cultivation.
7. Use according to claim 6, for the planting of strawberries.
CN202011466151.8A 2020-12-14 2020-12-14 Lactobacillus and application thereof in bio-organic fertilizer Pending CN112430162A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Publications (1)

Publication Number Publication Date
CN112430162A true CN112430162A (en) 2021-03-02

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107667797A (en) * 2017-09-21 2018-02-09 界首市靳莓果酒有限责任公司 A kind of implantation methods for improving strawberry sugar content
CN110129235A (en) * 2019-05-28 2019-08-16 青岛力力惠生物科技股份有限公司 A kind of heat-resisting plant lactobacillus and its application in agricultural production
CN112028692A (en) * 2020-09-16 2020-12-04 成都云图控股股份有限公司 Special biological organic fertilizer for saline-alkali soil and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107667797A (en) * 2017-09-21 2018-02-09 界首市靳莓果酒有限责任公司 A kind of implantation methods for improving strawberry sugar content
CN110129235A (en) * 2019-05-28 2019-08-16 青岛力力惠生物科技股份有限公司 A kind of heat-resisting plant lactobacillus and its application in agricultural production
CN112028692A (en) * 2020-09-16 2020-12-04 成都云图控股股份有限公司 Special biological organic fertilizer for saline-alkali soil and preparation method thereof

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Application publication date: 20210302