CN112391064A - 苯胺蓝染液 - Google Patents
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Abstract
本发明公开了一种苯胺蓝染液，其中，所述苯胺蓝染液包括蓝染剂和红染剂两部分，蓝染剂为苯胺蓝，所述红染剂包括丽春红和酸性品红，所述染液还包括磷钼酸染剂。本发明采用苯胺蓝染液结果准确，操作要求低；体积小分装明确，不仅节约了染色试剂，经济实用、便于携带且使用方便；快速混匀成套实用可以高效的实现胶原纤维的染色工作；可以快速弥补临床检测试剂的空缺，具有良好的推广前景。
Description
技术领域
本发明涉及一种苯胺蓝染液，属于苯胺蓝染液领域。
背景技术
苯胺蓝是一种混合酸性染料，为常用的生物染色剂，可用于神经组织、细胞、结缔组织等病理组织的染色。但苯胺蓝难溶于水，也不易溶于酒精，在染色时的效果也不易掌握，因此目前会采用多种原料混合的方式增大苯胺蓝的溶解度，固定染色效果。
随着病理学的快速发展，病理组织染色成为该领域关键技术之一，然而，由于细胞结果的复杂性，组织内容还存在多种特殊结构，如胶原纤维、网状纤维、弹力纤维、肌肉组织、脂肪、糖原、粘液、病理性沉淀(如铜、含铁血黄素等)、核酸等。胶原纤维是一类分布最广泛、含量最多的，且主要含有胶原蛋白和氨基酸(如甘氨酸、脯氨和羟脯氨酸等)的纤维组合物，广泛分布于各脏器内，在皮肤、巩膜和肌腱最为丰富。
苯胺蓝染色液常与丽春红品红染色液等配合使用对胶原纤维进行染色，染色后肌纤维呈红色，胶原纤维呈蓝色，主要用于区分胶原纤维和肌纤维。但现有的染色方法过程复杂，染色效果差，限制了其在临床上的大规模使用，且目前几乎没有成套的商业化染色产品。且苯胺蓝染液不适于大规格试剂，因此，开发设计一款小规格的苯胺蓝染液是十分必要的。
发明内容
针对现有技术存在的上述问题，本发明的目的是获得一种苯胺蓝染液。
为实现上述发明目的，本发明采用的苯胺蓝染液的技术方案如下：
所述苯胺蓝染液包括蓝染剂和红染剂两部分，蓝染剂为苯胺蓝。所述红染剂包括丽春红和酸性品红。所述染液还包括磷钼酸染剂。
优选的，所述蓝染剂为溶于乙酸的苯胺蓝，苯胺蓝：乙酸的质量比为0.1-1:80。
优选的，所述红染剂为溶于乙酸的丽春红和酸性品红，丽春红：酸性品红：乙酸的质量比为0.1-1：0.1-1:100。其中，乙酸为浓度0.2％的乙酸。
优选的，磷钼酸染剂为溶于蒸馏水的磷钼酸，磷钼酸：蒸馏水的质量比为1-10：100。
本发明的苯胺蓝染液的具体配比如下表1：
表1
上表中的数据可以根据使用时的情况搭配，在此不做组合的罗列。
作为本发明的优选实施方式，本发明的苯胺蓝染液使用时，先滴入红染剂，染色洗涤后再加入磷钼酸染剂，染色洗涤后最后加入蓝染剂。更优选的，使用时，先滴入红染剂，5-8min，水洗1-2min后再加入磷钼酸染剂，染色1-3min，水洗2-3min后最后加入蓝染剂，孵育5min，水洗2-3min。
为了便于使用并保证染液质量，所述苯胺蓝染液分装于50ml以下的容器中。
与现有技术相比，本发明采用苯胺蓝染液结果准确，操作要求低；体积小分装明确，不仅节约了染色试剂，经济实用、便于携带且使用方便；快速混匀成套实用可以高效的实现胶原纤维的染色工作；可以快速弥补临床检测试剂的空缺，具有良好的推广前景。
具体实施方式
下面结合实施例对本发明提供的苯胺蓝染液作进一步详细、完整地说明。下面描述的实施例是示例性的，仅用于解释本发明，而不能理解为对本发明的限制。
下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的实验材料如无特殊说明，均为市场购买得到。
本发明的苯胺蓝染液包括蓝染剂和红染剂两部分。其中蓝染剂为苯胺蓝，红染剂包括丽春红和酸性品红，所述染液还包括磷钼酸染剂。
实施例1
本发明的苯胺蓝染液的具体配比如下表2：
表2
本发明中的苯胺蓝染液的制备过程按照上表中的配方配置如下：
a)配置蓝染剂：称取定量的苯胺蓝溶解于蒸馏水，并加入乙酸，苯胺蓝：乙酸的质量比为0.1-1:80，乙酸为浓度0.2％的乙酸；
b)配置红染剂：称取定量的丽春红和酸性品红溶解于蒸馏水，并加入乙酸，丽春红：酸性品红：乙酸的质量比为0.1-1：0.1-1:100，乙酸为浓度0.2％的乙酸；
c)配置磷钼酸染剂：称取定量的磷钼酸溶解于蒸馏水，磷钼酸：蒸馏水的质量比为1-10：100；
d)过滤分装：将制备好的蓝染剂、红染剂和磷钼酸染剂过滤后，分装至50ml以下的容器中。
使用时，将样本固定于玻片表面，滴加1-2滴红染剂，染色5-8min，水洗1-2min；然后滴加1-2滴磷钼酸染剂，染色1-3min，水洗2-3min；再滴加1-2滴蓝染剂，孵育5min，水洗2-3min。
实施例2
电镜结果检测
通过对结缔组织、肌肉组织、神经组织分别进行染色，染色后进行电镜检测，可以看出纤维部分明显，染色效果优良。
最后有必要在此说明的是：以上实施例只用于对本发明的技术方案作进一步详细地说明，不能理解为对本发明保护范围的限制，本领域的技术人员根据本发明的上述内容作出的一些非本质的改进和调整均属于本发明的保护范围。
Claims (10)
1.一种苯胺蓝染液，其特征在于，所述苯胺蓝染液包括蓝染剂和红染剂两部分，蓝染剂为苯胺蓝。
2.根据权利要求1所述的苯胺蓝染液，其特征在于，所述红染剂包括丽春红和酸性品红。
3.根据权利要求1所述的苯胺蓝染液，其特征在于，所述染液还包括磷钼酸染剂。
4.根据权利要求1所述的苯胺蓝染液，其特征在于，所述蓝染剂为溶于乙酸的苯胺蓝，苯胺蓝：乙酸的质量比为0.1-1:80。
5.根据权利要求1所述的苯胺蓝染液，其特征在于，所述红染剂为溶于乙酸的丽春红和酸性品红，丽春红：酸性品红：乙酸的质量比为0.1-1：0.1-1:100。
6.根据权利要求4或5所述的苯胺蓝染液，其特征在于，乙酸为浓度0.2％的乙酸。
7.根据权利要求3所述的苯胺蓝染液，其特征在于，磷钼酸染剂为溶于蒸馏水的磷钼酸，磷钼酸：蒸馏水的质量比为1-10：100。
8.根据权利要求1所述的苯胺蓝染液，其特征在于，使用时，先滴入红染剂，染色洗涤后再加入磷钼酸染剂，染色洗涤后最后加入蓝染剂。
9.根据权利要求8所述的苯胺蓝染液，其特征在于，使用时，先滴入红染剂，5-8min，水洗1-2min后再加入磷钼酸染剂，染色1-3min，水洗2-3min后最后加入蓝染剂，孵育5min，水洗2-3min。
10.根据权利要求1所述的苯胺蓝染液，其特征在于，所述苯胺蓝染液分装于50ml以下的容器中。
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