CN112314607B - Polygonum bulbiferum growth regulator, direct-seeding mixture of polygonum bulbiferum and bulbifer and application thereof - Google Patents

Polygonum bulbiferum growth regulator, direct-seeding mixture of polygonum bulbiferum and bulbifer and application thereof Download PDF

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CN112314607B
CN112314607B CN202011338033.9A CN202011338033A CN112314607B CN 112314607 B CN112314607 B CN 112314607B CN 202011338033 A CN202011338033 A CN 202011338033A CN 112314607 B CN112314607 B CN 112314607B
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polygonum
growth regulator
bulbiferum
seeding
polygonum bulbiferum
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CN112314607A (en
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王智荣
谢俊杰
程媛
蒙阳
陆永国
邓强
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Dongguan Dongyangguang Cordyceps Research And Development Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
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  • Wood Science & Technology (AREA)
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  • Agronomy & Crop Science (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of traditional Chinese medicinal material planting, and particularly relates to a polygonum bulbiferum growth regulator and a direct-seeding mixture of polygonum bulbiferum and application of the growth regulator and the direct-seeding mixture of polygonum bulbiferum to polygonum bulbiferum planting. Specifically, the Polygonum nuciferum growth regulator comprises two or three of rooting agent, gibberellin and brassinolide; the direct seeding mixture of the polygonum bulbiferum bulbils comprises the polygonum bulbils treated by the growth regulator and the water-retaining agent. The direct seeding mixture of the polygonum bulbiferum and the pearl buds can effectively solve the problem of low emergence rate of the polygonum bulbiferum after the pearl buds are seeded.

Description

Polygonum bulbiferum growth regulator, direct-seeding mixture of polygonum bulbiferum and application thereof
Technical Field
The invention belongs to the technical field of traditional Chinese medicinal material planting, and particularly relates to a polygonum bulbiferum growth regulator and a direct-seeding mixture of polygonum bulbiferum and application of the growth regulator and the direct-seeding mixture of polygonum bulbiferum to polygonum bulbiferum planting.
Background
Polygonum bulbiferum L, also known as Scorpion seven, sorghum halepense and dyed cloth, is a perennial herb of Polygonum of Polygonaceae. The ecological floating island is widely distributed in Asia, North America and Europe, and mainly grows in grasslands, river beaches and shrubs with the elevation of 1200-5100 m, such as Sichuan, Tibet, Qinghai, Gansu, Yunnan, Jilin, inner Mongolia and Hubei in China. Polygonum bulboruifolium is a widely used Chinese herbal medicine, and has the functions of reducing fever, stopping diarrhea, regulating menstruation, astringing and stopping bleeding by using rhizome as a medicine; the rhizome and fruit are rich in starch, and can be used for brewing wine, and the young stem and leaf can be used as feed. The whole grass is mashed and made into powder or solution which can prevent and control crop pests. Besides being used as a traditional Chinese medicine, the rhizome of the polygonum bulbiferum is also a main feed for host larvae of cordyceps sinensis which is a rare medicinal material in China, so that the polygonum bulbiferum has higher economic value.
At present, the artificial planting propagation mode of the polygonum bulbiferum mainly adopts bulbils as main materials, the bulbils are planted on inflorescences, the bulbils and flowers grow synchronously, the bulbils can be attached to mother plant plants to germinate leaf buds when a mother body does not fall off, and the leaf buds fall into soil after being mature to grow new plants. The pearl buds are nutritive organs of the polygonum bulbiferum, are different from seeds of common plants, and have no embryo and endosperm of the plant seeds, so the environment required by the pearl buds in the processes of germination, growth, soil breaking and seedling formation is very harsh, and the seedling period is long. In the prior art, the polygonum bulbiferum is planted along with the collection, and generally in June of every year, ditching and drilling or carriage-lifting sowing are adopted for planting, and soil is covered after sowing. At the moment, the weather is hot, rainwater is less, and due to the fact that manual earthing is not uniform, under the conventional planting condition, the capacity of breaking soil and sprouting of the bulbels is very poor, the bulbels which do not sprout soil in time are extremely prone to dehydration and inactivation under the high-temperature and arid soil environment, the germination rate and seedling consistency of the bulbels of the polygonum persicum are seriously affected, and further great difficulty is brought to management of the polygonum persicum seedlings.
Disclosure of Invention
The invention aims to provide a polygonum bulbiferum growth regulator which can effectively promote the germination of polygonum bulbiferum buds and improve the rate of emergence after the treatment of the polygonum bulbiferum buds. The invention also provides a direct seeding mixture of the polygonum bulbil and the bulbil, which comprises the polygonum bulbil and the water-retaining agent which are treated by the growth regulator. According to the invention, the direct seeding mixture of the polygonum bulbiferum and the pearl buds is combined with a specific mode for seeding, so that the problem of low emergence rate after the sowing of the polygonum bulbiferum and the pearl buds can be effectively solved.
Specifically, in one aspect, the invention provides a polygonum bulbiferum growth regulator which comprises two or three of a rooting agent, gibberellin and brassinolide.
In some embodiments, the polygonum bulbiferum growth regulator consists of two or three of a rooting agent, gibberellin, brassinolide.
In some embodiments, the rooting agent consists of 30% indole acetic acid and 20% naphthalene acetic acid.
In some embodiments, the rooting agent has a total effective composition of 50%.
In some embodiments, the rooting agent is a dust.
In some embodiments, the Polygonum nuciferum growth regulator is an aqueous solution.
In some embodiments, the rooting agent concentration is 50-200 mg/L; in other embodiments, the rooting agent is at a concentration of 50-150 mg/L; in some preferred embodiments, the rooting agent concentration is 100 mg/L.
In some embodiments, the gibberellin concentration is 3-10 mg/L; in other embodiments, the gibberellin concentration is 3-6 mg/L; in some preferred embodiments, the gibberellin concentration is 4 mg/L.
In some embodiments, the brassinolide concentration is 0.15 to 0.4 mg/L; in some preferred embodiments, the brassinolide concentration is 0.2 mg/L.
In some embodiments, the rooting agent, gibberellin, or brassinolide is dissolved in 95% ethanol prior to formulating with the aqueous solution of Polygonum nuciferum growth regulator. The water solution of the polygonum bulbiferum growth regulator can be used for conveniently treating the polygonum bulbiferum and promoting the germination of the bulbiferum.
In some specific embodiments, the polygonum nuciferum growth regulator aqueous solution comprises 100mg/L of rooting powder and 4mg/L of gibberellin.
In some specific embodiments, the aqueous solution of the Polygonum nuciferum growth regulator comprises 100mg/L of rooting powder and 0.2mg/L of brassinolide.
In some specific embodiments, the aqueous solution of the Polygonum nuciferum growth regulator comprises 4mg/L gibberellin and 0.2mg/L brassinolide.
In some specific embodiments, the water solution of the polygonum bulbiferum growth regulator comprises 100mg/L of rooting powder, 4mg/L of gibberellin and 0.2mg/L of brassinolide.
In another aspect, the invention provides a direct seeding mixture of polygonum bulbiferum and bulbels, which comprises the bulbels of polygonum bulbiferum treated by a growth regulator and a water-retaining agent, wherein the bulbels of polygonum bulbiferum treated by the growth regulator are prepared by the following steps: and uniformly spraying the prepared growth regulator solution on the surfaces of the bulbels, and airing the bulbels for later use.
In some embodiments, the growth regulator comprises one, two or three of a rooting agent, gibberellin, brassinolide.
In some embodiments, the growth regulator consists of one, two or three of a rooting agent, gibberellin, brassinolide.
In some embodiments, the rooting agent consists of 30% indolylacetic acid and 20% naphthylacetic acid, with a total active ingredient of 50%.
In some embodiments, the rooting agent is a dust.
In some embodiments, the growth regulator solution is an aqueous solution;
in some embodiments, the rooting agent concentration is 50-200 mg/L; in other embodiments, the rooting agent is at a concentration of 50-150 mg/L; in some preferred embodiments, the rooting agent concentration is 100 mg/L.
In some embodiments, the gibberellin concentration is 3-10 mg/L; in other embodiments, the gibberellin concentration is 3-6 mg/L; in some preferred embodiments, the gibberellin concentration is 4 mg/L.
In some embodiments, the brassinolide concentration is 0.15 to 0.4 mg/L; in some preferred embodiments, the brassinolide concentration is 0.2 mg/L.
In some embodiments, the rooting agent, gibberellin, or brassinolide is dissolved in 95% ethanol prior to formulating with the aqueous solution of Polygonum nuciferum growth regulator.
In some specific embodiments, the polygonum nuciferum growth regulator aqueous solution comprises 100mg/L of rooting powder and 4mg/L of gibberellin.
In some specific embodiments, the aqueous solution of the Polygonum nuciferum growth regulator comprises 100mg/L of rooting powder and 0.2mg/L of brassinolide.
In some specific embodiments, the water solution of the polygonum bulbiferum growth regulator comprises 4mg/L gibberellin and 0.2mg/L brassinolide.
In some specific embodiments, the aqueous solution of the Polygonum nuciferum growth regulator comprises 100mg/L rooting powder, 4mg/L gibberellin and 0.2mg/L brassinolide.
In some embodiments, every 15kg of the pearl buds are treated with 500mL of the growth regulator solution, namely, the ratio of the pearl buds of the polygonum bulbil to the growth regulator solution in the direct seeding mixture of the pearl buds is as follows: 15kg/500 mL.
In some embodiments, the Polygonum criopolitanum bead size is 7 mesh or 7-10 mesh.
In some embodiments, the polygonum bulbil is screened to obtain the bulbil with the particle size of more than or equal to 7 meshes or 7-10 meshes by the following steps: removing leaves, flower spikes and pearls with poor shrivelled pearls from fresh pearls, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes and less than 10 meshes after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes or 7-10 meshes for sowing the pearls of the polygonum bulbil.
In some embodiments, the water retaining agent is polyacrylamide, preferably the water retaining agent is in powder form.
In some embodiments, the mass ratio of the polygonum bulbiferum to the water-retaining agent is 10-15: 4.
on the other hand, the invention provides an application of the polygonum bulbiferum growth regulator or the direct-seeding mixture of polygonum bulbiferum buds in the planting of polygonum bulbiferum buds.
In another aspect, the present invention provides a method for sowing seeds by using the direct seeding mixture of the polygonum bulbiferum, comprising the following steps:
adopting a ditching and drilling seeding mode, uniformly drilling the direct seeding mixture of the polygonum bulbiferum and the pearl buds into the van surface ditch, wherein the using amount of the pearl buds is 10-15 kg/mu, and covering the drilling pearl bud planting ditch with peat soil after seeding, wherein the covering thickness is 2-3 cm. The peat soil is loose in soil property and light in density, has huge water absorption and water retention performances, and can provide a long-time humid environment for the germination and growth of the bulbels in a planting period with less rainwater by matching the peat soil with the water retention agent, so that the germination of the bulbels is promoted.
And (4) watering to moisten and soak the inside of the planting ditch according to the weather conditions after the seeds are sown and covered with soil.
The rooting agent is a substance required for regulating the growth and development of plants, and comprises the main components of indoleacetic acid, indolebutyric acid, naphthylacetic acid, royal root and the like. The preferable rooting agent is powder, and the rooting agent is preferably composed of indoleacetic acid and naphthylacetic acid. The more preferable rooting agent of the invention is rooting powder consisting of 30% of indoleacetic acid and 20% of naphthylacetic acid, wherein the effective component is 50%.
The water-retaining agent is high water-absorbing resin, is a functional polymer material with extremely strong water absorption capacity, can absorb fertilizers and pesticides, and can be slowly released to increase the fertilizer efficiency and the pesticide effect. Examples of water retention agents include, but are not limited to: one is acrylamide-acrylate copolymer (polyacrylamide, sodium polyacrylate, potassium polyacrylate, ammonium polyacrylate, etc.); another class is starch graft acrylate copolymeric cross-links (starch graft acrylates). The preferred water retaining agent of the present invention is polyacrylamide.
The invention has the beneficial effects that:
(1) the polygonum bulbiferum bulbels are compounded with two or three growth regulators, and then are mixed with a water-retaining agent to obtain a bulbil direct seeding mixture, and the mixture is sown.
(2) The method for pretreating the polygonum bulbiferum before sowing is simple and suitable for popularization.
Detailed Description
The following are preferred embodiments of the present invention, and the present invention is not limited to the following preferred embodiments. It should be noted that various changes and modifications based on the inventive concept herein will occur to those skilled in the art and are intended to be included within the scope of the present invention. The reagents and apparatus used are not indicated by the manufacturer, and are all conventional products commercially available.
The rooting powder is purchased from Beijing Aibibidi Biotechnology Limited and consists of 30 percent of indoleacetic acid and 20 percent of naphthylacetic acid, and the total effective component is 50 percent; the water-retaining agent powder is purchased from Aisen group of France, and the chemical component is polyacrylamide.
The polygonum bulbiferum bulbil used in the invention is collected in Shoutong city in Yunnan province, the bulbil in the embodiment of the invention is planted in the middle and late ten days of 6 months, the elevation of the planting field is 1800 and 2500 meters, and the environmental temperature is 15-28 ℃.
Examples
The following is an example of the seeding of the pearl buds of Polygonum hydropiper.
Example 1
(1) Removing impurities from the pearl buds and grading: removing leaves, spica and bulbels with poor shriveling in fresh bulbels by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of bulbels with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the bulbels with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 100mg of rooting powder in 10mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a rooting powder growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of the bulbels, placing the bulbels at a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a sowing mode of ditching and drilling to uniformly drill the mixture of the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted in mu (666.7 m) 2 ) The dosage is 15kg, and peat soil is used to cover the drilling pearl buds after seedingThe planting furrows are covered with the thickness of 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The density and rate of emergence 7 days, 14 days, 21 days, 28 days after the seeding of the bulbels are shown in tables 1 and 2, respectively.
Example 2
(1) Bead bud impurity removal and classification: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 5mg of gibberellin in 5mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a gibberellin growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of bulbels, placing the bulbels in a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a sowing mode of ditching and drilling to uniformly drill the mixture of the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted in mu (666.7 m) 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Example 3
(1) Removing impurities from the pearl buds and grading: removing leaves, spica and bulbels with poor shriveling in fresh bulbels by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of bulbels with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the bulbels with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 0.2mg of brassinolide in 5mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a brassinolide growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of the bulbels, placing the bulbels at a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a ditching and drilling sowing mode to uniformly drill the mixture of the bulbels to be sown into the grooves on the compartment surface, wherein the bulbels are 666.7m per mu 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Example 4
(1) Removing impurities from the pearl buds and grading: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 50mg of rooting powder and 2mg of gibberellin in 10mL of 95% alcohol, and then diluting to 500mL with water to obtain a growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of the bulbels, placing the bulbels at a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a ditching and drilling sowing mode to uniformly drill the mixture of the bulbels to be sown into the grooves on the compartment surface, wherein the bulbels are 666.7m per mu 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Example 5
(1) Removing impurities from the pearl buds and grading: removing leaves, spica and bulbels with poor shriveling in fresh bulbels by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of bulbels with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the bulbels with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 50mg of rooting powder and 0.1mg of brassinolide in 10mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of the bulbels, placing the bulbels at a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a sowing mode of ditching and drilling to uniformly drill the mixture of the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted in mu (666.7 m) 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Example 6
(1) Removing impurities from the pearl buds and grading: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 2mg of gibberellin and 0.1mg of brassinolide in 10mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of bulbels, placing the bulbels in a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels obtained in the step (3) with 4kg of water-retaining agent powder to obtain a bulbil mixture to be sowed;
adopting a sowing mode of ditching and drilling to uniformly drill the mixture of the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted in mu (666.7 m) 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows for drilling the pearl buds after sowing, and the covering thickness is 2-3 cm;
(5) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Example 7
(1) Removing impurities from the pearl buds and grading: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) preparation of growth regulator: dissolving 50mg of rooting powder, 2mg of gibberellin and 0.1mg of brassinolide in 10mL of 95% alcohol, and then adding water to a constant volume of 500mL to obtain a growth regulator solution;
(3) uniformly spraying 500mL of the growth regulator solution prepared in the step (2) on the surface of 15kg of bulbels, placing the bulbels in a ventilated place, and airing the moisture on the surface of the bulbels;
(4) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(5) adopting a ditching and drilling sowing mode to uniformly drill the mixture of the bulbels to be sown into the grooves on the compartment surface, wherein the bulbels are 666.7m per mu 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(6) watering after covering soil to wet and soak the planting furrows.
The density and rate of emergence 7 days, 14 days, 21 days, 28 days after the seeding of the bulbels are shown in tables 1 and 2, respectively.
Comparative example
(1) Removing impurities from the pearl buds and grading: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) uniformly spraying 500mL of clear water on the surface of 15kg of bulbels, placing the bulbels at a ventilated place, and airing the moisture on the surface of the bulbels;
(3) fully mixing the dried bulbels in the step (3) with 4kg of water-retaining agent powder to obtain a bulbel mixture to be sowed;
(4) adopting a sowing mode of ditching and drilling to uniformly drill the mixture of the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted in mu (666.7 m) 2 ) The using amount is 15kg, and peat soil is used for covering the planting furrows of the drill seeding pearl buds after seeding, and the covering thickness is 2-3 cm;
(5) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Examples of conventional planting
(1) Bead bud impurity removal and classification: removing leaves, flower spikes and pearls with poor shrivelled pearls in fresh pearls by using a winnowing machine, grading by using a standard 7-mesh sieve (2.80mm) and a standard 10-mesh sieve (2.00mm), obtaining 3 types of pearls with the size distribution of more than or equal to 7 meshes, 7-10 meshes, less than 10 meshes and the like after grading, and selecting the pearls with the granularity of more than or equal to 7 meshes for sowing;
(2) adopting a sowing mode of ditching and drilling to uniformly drill the pearl buds to be sown into the ditch of the compartment surface, wherein the pearl buds are planted per mu (666.7 m) 2 ) The using amount is 15kg, and after sowing, the fine soil nearby is taken out to cover the planting furrows for drilling the bulbels, and the covering thickness is 2-3 cm;
(3) watering after covering soil to wet and soak the planting furrows.
The seedling emergence density and the seedling emergence rate of 7 days, 14 days, 21 days and 28 days after the seeding of the bead buds are respectively shown in the table 1 and the table 2.
Statistical results of density and rate of emergence of the pearl buds of examples 1 to 7, comparative example and conventional planting example
Tracking statistics are carried out on the sprouting condition of the pearl buds of the examples 1 to 7, the comparative example and the conventional planting example respectively. Statistics are carried out on the density of the seedling emergence of the bulbels at 7 days, 14 days, 21 days and 28 days after sowing, and the results are shown in the following table 1:
TABLE 1
Figure BDA0002797753670000081
Figure BDA0002797753670000091
After grading the bulbils, the thousand seed weight of the bulbils with the grain size of more than or equal to 7 meshes for planting is about 25g, the using amount of the bulbils per mu (666.7m2) is 15kg, and the seeding amount of the bulbils is 900 strains/m calculated from the calculation 2 On the basis of the results, the seedling emergence rate is converted according to the seedling emergence density of the pearl buds, and the results are shown in the following table 2:
TABLE 2
Examples 7-day emergence (%) Emergence rate of 14 days (%) Emergence rate (%) for 21 days Emergence rate (%) for 28 days
Example 1 0.56％ 39.11％ 56.44％ 60.22％
Example 2 0.78％ 44.44％ 53.78％ 60.00％
Example 3 0.89％ 45.78％ 57.78％ 62.78％
Example 4 0.89％ 48.33％ 65.33％ 69.78％
Example 5 0.56％ 51.33％ 65.00％ 71.22％
Example 6 0.67％ 51.00％ 66.11％ 73.00％
Example 7 0.56％ 61.22％ 82.33％ 87.67％
Comparative examples 0.67％ 28.89％ 38.44％ 46.11％
Examples of conventional planting 0.67％ 13.33％ 16.22％ 21.67％
As can be seen from the above table, there was no great difference in the rate of emergence of Polygonum nuciferum at 7 days after the planting of examples 1-7, comparative example and conventional planting example, but the results of examples 1-7 were significantly higher than those of comparative example and conventional planting example at 14 days, 21 days and 28 days thereafter. In comparative examples 1 to 3, the seeding method of the direct seeding mixture of the pearl buds was adopted after the treatment of different growth regulators, and the emergence rates of the pearl buds were not significantly different at 14 days, 21 days and 28 days after the seeding, but in comparative examples 4 to 6, the seeding method of the direct seeding mixture of the pearl buds was adopted after the treatment of the two-by-two combination of different growth regulators, and the emergence rates of the pearl buds were significantly improved compared with the treatment with a single growth regulator. Furthermore, the rooting powder, gibberellin and brassinolide are adopted in the embodiment 7 to be used in a compounding mode, the effect is optimal, after 14 days, the emergence rate of the bulbil is improved by more than 15.44% compared with that of a single growth regulator, is improved by 32.33% compared with that of a comparative embodiment, and is improved by 47.89% compared with that of a conventional planting embodiment; after 28 days, the germination rate is improved by 41.56% compared with that of the comparative example and is improved by 66% compared with that of the conventional planting example, which shows that the compound treatment of three growth regulators of rooting powder, gibberellin and brassinolide and the germination and emergence of the pearl buds can be obviously promoted by combining with the sowing mode of a pearl bud direct seeding mixture.
In conclusion, the direct pearl sprout seeding mixture disclosed by the invention is used for seeding in a specific mode, so that the growth microenvironment after pearl sprout seeding of the polygonum bulbiferum can be improved, a moist and loose germination growth environment is provided for the pearl sprouts, the problems of low pearl sprout germination rate and seedling rate in the planting process of the polygonum bulbiferum in a field are solved, and the stress resistance of the seedling of the polygonum bulbiferum is improved.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (3)

1. The application of a water solution of a polygonum bulbiferum growth regulator in the planting of polygonum bulbiferum is characterized in that the active ingredients of the water solution of the polygonum bulbiferum growth regulator consist of a rooting agent, gibberellin and brassinolide; the concentration of the gibberellin is 4 mg/L; the concentration of the brassinolide is 0.2 mg/L; dissolving the rooting agent, gibberellin and brassinolide with alcohol with the concentration of 95 percent and the volume accounting for 2 percent of the total volume of the water solution, and then adding water to prepare a polygonum bulborum growth regulator water solution;
the total effective components of the rooting agent are 50%, and the effective components in the rooting agent consist of 30% of indoleacetic acid and 20% of naphthylacetic acid in the rooting agent; the concentration of the rooting agent is 100 mg/L;
the using method of the polygonum bulbiferum growth regulator aqueous solution comprises the following steps: uniformly spraying the prepared water solution of the polygonum bulbiferum growth regulator on the surfaces of the polygonum bulbiferum buds, and airing the buds for later use; then fully mixing with a water-retaining agent to obtain a direct-seeding mixture of the bulbophyllum nuciferum; treating every 15kg of the bulbels with 500mL of growth regulator solution; the granularity of the bulbiferous buds of the polygonum bulbiferum is more than or equal to 7 meshes; the water-retaining agent is polyacrylamide; the mass ratio of the bulbophyllum to the water-retaining agent is 10-15: 4.
2. the use according to claim 1, the water retaining agent being a powdered polyacrylamide.
3. The use according to claim 1, further comprising a seeding step:
adopting a ditching and drilling seeding mode, uniformly drilling the direct seeding mixture of the polygonum bulbiferum and the pearl buds into the van surface ditch, wherein the using amount of the pearl buds is 10-15 kg/mu, and covering the drilling pearl bud planting ditch with peat soil after seeding, wherein the covering thickness is 2-3 cm.
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