CN112314509B - Deep sea macro organism fidelity culture device and culture method - Google Patents
Deep sea macro organism fidelity culture device and culture method Download PDFInfo
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- CN112314509B CN112314509B CN202011497303.0A CN202011497303A CN112314509B CN 112314509 B CN112314509 B CN 112314509B CN 202011497303 A CN202011497303 A CN 202011497303A CN 112314509 B CN112314509 B CN 112314509B
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- 238000012136 culture method Methods 0.000 title description 6
- 238000000034 method Methods 0.000 claims abstract description 19
- 230000008569 process Effects 0.000 claims abstract description 18
- 238000005070 sampling Methods 0.000 claims abstract description 15
- 238000005057 refrigeration Methods 0.000 claims abstract description 14
- 230000007246 mechanism Effects 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 210000001503 joint Anatomy 0.000 claims description 15
- 238000012546 transfer Methods 0.000 claims description 11
- 239000013535 sea water Substances 0.000 claims description 9
- 229910000619 316 stainless steel Inorganic materials 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 125000004122 cyclic group Chemical group 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 238000012364 cultivation method Methods 0.000 claims 1
- 238000011065 in-situ storage Methods 0.000 abstract description 5
- 230000000007 visual effect Effects 0.000 abstract description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 5
- 235000017491 Bambusa tulda Nutrition 0.000 description 5
- 241001330002 Bambuseae Species 0.000 description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 5
- 239000011425 bamboo Substances 0.000 description 5
- 238000012258 culturing Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/047—Liquid pumps for aquaria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Automation & Control Theory (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a deep sea macro organism fidelity culture device which comprises a support adjusting module, a control module, a culture module and a refrigeration module, wherein the support adjusting module is fixedly arranged on a container, the culture module is rotatably arranged on the support adjusting module, the refrigeration module is arranged on the culture module, and the control module is respectively connected with the culture module and the refrigeration module. The deep-sea macro-organism fidelity culture device can monitor the state parameters of the macro-organism culture process, and the adopted computer display interface is concise and visual, so that the temperature curve and the pressure curve in the deep-sea macro-organism sampling device can be accurately observed; the temperature and pressure threshold values are set by a computer, the refrigeration power of the refrigerator is controlled, the low-temperature requirement is realized, the pressurizing pump is controlled to realize the high-pressure requirement, and the environment pressure and temperature of the deep-sea macro organism are ensured to be similar to the in-situ pressure and temperature.
Description
Technical Field
The invention relates to a submarine organism culture device, in particular to a deep-sea macro organism fidelity culture device and a culture method.
Background
Research results show that the mining of marine biological resources is helpful for searching brand new species and life mechanisms; meanwhile, marine organisms are used as ideal medicine and industrial material resources, and have potential economic value. If the in-situ culture experiment of the submarine organism can be carried out, the submarine macro organism living body sample can be effectively stored, and more comprehensive data support and basis can be provided for revealing the deep-sea organism geochemical circulation mechanism. Patent CN111471576a proposes a deep sea microorganism in-situ culture device with pressure compensation, which can perform enrichment culture and recovery of microorganisms in a deep sea environment, but only maintains the in-situ pressure of the microorganisms, and cannot control the temperature change in the recovery process. Patent CN111109159a proposes a carrying type deep-sea macro-organism pressure-maintaining sampling device which can completely transfer captured macro-organisms, but cannot preserve heat and transfer, and is not suitable for storing deep-sea macro-organism samples for a long time with fidelity. This will have a great influence on accurate studies of the living situation of the living being in the seabed area, the seabed environment, etc. Therefore, development of a deep-sea macro-organism fidelity culture device with simple structure, convenient operation and automation is needed, so that a high-quality seabed in-situ sample is provided for scientific researches such as life evolution of a deep-sea macro-organism community.
Disclosure of Invention
In order to solve the technical problems, the invention provides the deep-sea macro-organism fidelity culture device which is simple in structure, convenient to operate and high in automation degree, and provides a safe and reliable deep-sea macro-organism fidelity culture method.
The technical scheme for solving the problems is as follows: the deep sea macro organism fidelity culture device comprises a support adjusting module, a control module, a culture module and a refrigeration module, wherein the support adjusting module is fixedly arranged on a container, the culture module is rotatably arranged on the support adjusting module, the refrigeration module is arranged on the culture module, and the control module is respectively connected with the culture module and the refrigeration module;
The culture module comprises a pressure maintaining cylinder and an end cover, wherein the pressure maintaining cylinder is a semi-closed cylinder with an opening at one end, the opening end of the pressure maintaining cylinder is sealed by the end cover, a watertight joint II is arranged on the outer end face of the end cover, the watertight joint II is connected with the control module, a watertight joint I connected with the watertight joint II is arranged on the inner end face of the end cover, the watertight joint I is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in the inner cavity of the pressure maintaining cylinder, a butt joint hole for butt joint with the sampling module and a through hole for a macro-organism to pass through are arranged in the center of the end cover, a flap sealing mechanism for sealing or opening the through hole is arranged in the inner cavity of the pressure maintaining cylinder, a horn mouth is arranged on the flap sealing mechanism, and a switch valve for driving the flap sealing mechanism is arranged on the end cover; the end cover is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder is connected with a pressure gauge, an overflow valve and a high-pressure valve which are arranged on the outer end surface of the end cover through the high-pressure pipe connecting holes;
The automatic bait delivery device is arranged on the end cover and is communicated with the inner cavity of the pressure maintaining cylinder through a high-pressure pipe connecting hole;
The bottom of the pressure maintaining cylinder is provided with a drainage device, the drainage device comprises a high-pressure ball valve, a drainage cylinder and a drainage valve, one end of the high-pressure ball valve is communicated with the inner cavity of the pressure maintaining cylinder, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder, and the other end of the drainage cylinder is connected with the drainage valve; the bottom of the inner cavity of the pressure maintaining cylinder is provided with a filter plate for limiting the individual size of the deep-sea submarine organisms, and the filter plate is provided with a filter hole;
The refrigerating module comprises a condensing tube and a refrigerating machine, the condensing tube is arranged in the inner cavity of the pressure maintaining cylinder, the side surface of the pressure maintaining cylinder is provided with a connecting hole for the outlet and the inlet of the condensing tube, one end of the refrigerating machine is connected with the inlet of the condensing tube, the other end of the refrigerating machine is connected with the outlet of the condensing tube, and the refrigerating machine is connected with the control module.
The control module comprises a computer and a controller; the computer is connected with the controller, and the controller is connected with the watertight joint II; the display of the computer comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button.
Above-mentioned macroscopical biological fidelity culture apparatus in deep sea, support adjusting module includes base, support frame, motor, driven gear, the base mounting is on the container, and bilateral symmetry sets up two support frames on the base, pressurize section of thick bamboo side symmetry is equipped with two shoulders, all installs the axis of rotation on every shoulder, and axis of rotation one end is installed on the shoulder that corresponds the side, and the axis of rotation other end passes through the bearing to be installed in the bearing frame at the support frame top that corresponds the side, and fixed in one of them axis of rotation is equipped with the driving gear, and the driving gear meshes with the driven gear that is located its below, and driven gear passes through the transmission shaft and links to each other with the motor of fixed mounting on the support frame, and the motor is connected with the controller.
Above-mentioned deep sea macroscopical biological fidelity culture apparatus, the material that pressure maintaining section of thick bamboo, water drainage section of thick bamboo used is 316 stainless steel, and pressure maintaining section of thick bamboo, water drainage section of thick bamboo, high-pressure camera, high-pressure lamp, pressure sensor, temperature sensor, overflow valve and high-pressure valve bear the upper limit of pressure more than 110MPa.
According to the deep sea macro organism fidelity culture device, the rotating speed of the motor is 1450r/min, the number of teeth of the driving gear is 24, and the number of teeth of the driven gear is 72.
A deep sea macro organism fidelity culture method comprises the following steps:
(1) The pressurizing process comprises the following steps: before the sampling module is in butt joint with the culture module, injecting seawater into the culture module, injecting high-pressure water into the culture module through a high-pressure valve on a connecting end cover of a booster pump, measuring the real-time pressure in the culture module by a pressure sensor, outputting a pressure signal to a computer, displaying a real-time pressure value by a display of the computer, and closing the high-pressure valve when the pressure reaches the requirement;
(2) The refrigerating process comprises the following steps: the low-temperature water in the refrigerator flows into the condenser pipe through the condenser pipe inlet, so that the high-pressure seawater in the pressure maintaining cylinder is cooled, the water in the condenser pipe flows into the refrigerator through the condenser pipe outlet, the cyclic utilization is realized, the temperature sensor measures the real-time temperature in the culture module, the real-time temperature signal is output to the computer, the display of the computer displays the real-time temperature value, and the temperature in the macro biological culture module is maintained at 2-4 ℃;
(3) The transfer process comprises the following steps: the sampling module is in butt joint with the culture module through a butt joint hole on the end cover, a through hole on the end cover is opened through the turning plate sealing mechanism, macro organisms in the sampling module are transferred into the inner cavity of the pressure maintaining cylinder through the through hole, and after the macro organisms are completely transferred, the macro organisms pass through the through hole on the closed end cover of the turning plate sealing mechanism;
(4) The culture process comprises the following steps: after the macro organism is completely transferred to the culture module, the temperature and the pressure in the pressure maintaining cylinder are monitored in real time through a display of a computer, when the pressure is lower than a set threshold value, the input end of the controller receives a real-time pressure signal from the pressure sensor, and the output end of the controller outputs a control signal to control the pressurizing pump to pressurize; when the temperature is higher than a set threshold value, the input end of the controller receives a real-time temperature signal from the temperature sensor, the output end of the controller outputs a control signal to control the refrigeration power of the refrigerator, so that the temperature in the macro biological culture module is maintained at 2-4 ℃; automatically delivering the baits into the pressure maintaining cylinder through the automatic bait delivery device;
(5) And (3) drainage: after the macro-organisms are cultured in the culture module for a period of time, replacing water in the pressure maintaining cylinder, controlling a motor to drive a driven gear to rotate through a controller, meshing the driven gear with the driving gear to drive the driving gear to rotate, and driving a rotating shaft to rotate through a rotating gear to drive the culture module to rotate by 90 degrees, so that the culture module is horizontally placed, a high-pressure ball valve at the bottom of the pressure maintaining cylinder is opened, water in the pressure maintaining cylinder flows out into a drainage cylinder, and seawater in the pressure maintaining cylinder is transferred in batches, so that no pressure drop transfer is realized; after the transfer is completed, the high-pressure ball valve is closed, the driven gear is driven to rotate by the motor controlled by the controller, so that the culture module is driven to rotate by 90 degrees, high-pressure water is injected into the culture by the booster pump, and the steps (1) and (2) and (4) are continued.
The invention has the beneficial effects that:
1. The deep-sea macro-organism fidelity culture device can monitor the state parameters of the macro-organism culture process, and the adopted computer display interface is concise and visual, so that the temperature curve and the pressure curve in the deep-sea macro-organism sampling device can be accurately observed.
2. The deep-sea macro-organism fidelity culture device of the invention sets the temperature and the pressure threshold value through the computer, controls the refrigeration power of the refrigerator, realizes the low-temperature requirement, controls the pressurizing pump to realize the high-pressure requirement, and can ensure that the environmental pressure and the temperature of the deep-sea macro-organism are approximate to the original pressure and the original temperature of the deep-sea macro-organism.
3. The deep sea macro-organism fidelity culturing device can realize long-time culturing of macro-organisms, water and macro-organism excreta in the culturing device can be discharged through designing the water discharging device, and the water in the culturing device is replaced regularly, so that the whole process keeps no pressure drop.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a right side view of the three-dimensional structure of the present invention.
FIG. 3 is a schematic diagram showing the structure of the cultivation process according to the present invention.
Fig. 4 is a schematic perspective view of an end cap of a pressure maintaining cylinder according to the present invention.
Fig. 5 is a schematic view of the drainage process according to the present invention.
Fig. 6 is a schematic view of the drainage process according to the present invention.
Fig. 7 is a schematic diagram of the structure of the flap seal mechanism and the gear mechanism of the present invention.
FIG. 8 is a flow chart of the method of operation of the deep sea macrobiosis fidelity culture system of the present invention.
Detailed Description
The invention is further described below with reference to the drawings and examples.
As shown in fig. 1-8, the deep sea macro organism fidelity culture device comprises a support adjusting module 7, a control module, a culture module 6 and a refrigeration module 4, wherein the support adjusting module 7 is fixedly arranged on a container, the culture module 6 is rotatably arranged on the support adjusting module 7, the refrigeration module 4 is arranged on the culture module 6, and the control module is respectively connected with the culture module 6 and the refrigeration module 4.
The culture module 6 comprises a pressure maintaining cylinder 609 and an end cover 606, the pressure maintaining cylinder 609 is a semi-closed cylinder with one end open, the open end of the pressure maintaining cylinder 609 is sealed by the end cover 606, the outer end surface of the end cover 606 is provided with a watertight joint II 601, the watertight joint II 601 is connected with the control module, the inner end surface of the end cover 606 is provided with a watertight joint I611 connected with the watertight joint II 601, the watertight joint I611 is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in the inner cavity of the pressure maintaining cylinder 609, the center of the end cover 606 is provided with a butt joint hole 613 for being in butt joint with the sampling module and a through hole 614 for allowing macro organisms to pass through, the inner cavity of the pressure maintaining cylinder 609 is provided with a turnover plate sealing mechanism 607 for sealing or opening the through hole 614, and the end cover 606 is provided with a switch valve 605 for driving the turnover plate sealing mechanism 607; the end cover 606 is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder 609 is connected with a pressure gauge 612, an overflow valve 604, a high-pressure valve 603 and an exhaust valve 608 which are arranged on the outer end surface of the end cover 606 through the high-pressure pipe connecting holes. The end cover 606 is provided with an automatic bait delivery device 602, and the automatic bait delivery device 602 is communicated with the inner cavity of the pressure maintaining cylinder 609 through a high-pressure pipe connecting hole.
The flap sealing mechanism 607 comprises a flap valve seat 103, a flap valve cover 101, a sealing ring I102, a torsion spring 104 and a flap shaft 105, the flap valve seat 103 is in sealing connection with a pressure maintaining cylinder 609 through the sealing ring 102, a valve hole is formed in the flap valve seat 103, the flap shaft 105 is arranged at the valve hole, the torsion spring 104 is arranged on the flap shaft 105, the flap valve cover 101 is hinged with the flap valve seat 103 through the flap shaft 105, the shape of the hinge position of the flap valve seat 103 is gear-shaped, and the flap valve seat 103 is opened and closed through the gear mechanism 9.
The gear mechanism 9 comprises an inflation interface 901, a gear rod 903, a gear rod accommodating cavity and an O-shaped sealing ring II 902, wherein the gear rod accommodating cavity is positioned in the outer wall of the pressure maintaining cylinder 609, the inflation interface 901 is arranged on the pressure maintaining cylinder 609 and is communicated with the gear rod accommodating cavity, the gear rod 903 is arranged in the gear rod accommodating cavity, the outer diameter of the gear rod 903 is matched with the inner diameter of the gear rod accommodating cavity, the gear rod 903 can move in the gear rod accommodating cavity, one end of the gear rod 903 far away from the inflation interface 901 is provided with teeth, the teeth are meshed with the hinged part of the flap valve seat 103, the inflation interface 901 is connected in the gear mechanism 9 through a pressurizing pump, the pressure maintaining cylinder 609 is pressurized, the gear rod 903 moves left under the action of pressure, so that the flap valve cover 101 of the flap sealing valve mechanism is driven to rotate clockwise, and the outlet of the pressure maintaining cylinder 609 is opened; the space between the gear rod 903 and the inner wall of the gear rod accommodating chamber is sealed by an O-ring seal ii 902.
The bottom of the pressure maintaining cylinder 609 is provided with a drainage device 5, the drainage device 5 comprises a high-pressure ball valve 502, a drainage cylinder 501 and a drainage valve 503, one end of the high-pressure ball valve 502 is communicated with the inner cavity of the pressure maintaining cylinder 609, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder 501, and the other end of the drainage cylinder 501 is connected with the drainage valve 503; the bottom of the inner cavity of the pressure maintaining cylinder 609 is provided with a filter plate 610 for limiting the individual size of the deep sea submarine organisms, and the filter plate 610 is provided with a filter hole 616.
The refrigerating module 4 comprises a condensing tube 402 and a refrigerating machine 401, the condensing tube 402 is arranged in the inner cavity of the pressure maintaining cylinder 609, the side surface of the pressure maintaining cylinder 609 is provided with a connecting hole for the outlet and the inlet of the condensing tube 402, one end of the refrigerating machine 401 is connected with the inlet of the condensing tube 402, the other end of the refrigerating machine 401 is connected with the outlet of the condensing tube 402, and the refrigerating machine 401 is connected with the control module.
The control module comprises a computer 1 and a controller 2; the computer 1 is connected with the controller 2 through a cable 3, and the controller 2 is connected with the watertight joint II 601 through the cable 3; the display of the computer 1 comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer 1 comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button.
The support adjusting module 7 comprises a base 701, a support frame 702, a motor 704 and a driven gear 705, wherein the base 701 is arranged on a container, two support frames 702 are symmetrically arranged on two sides of the base 701, two shaft shoulders 8 are symmetrically arranged on the side faces of the pressure maintaining cylinder 609, each shaft shoulder 8 is provided with a rotating shaft 801, one end of each rotating shaft 801 is arranged on the corresponding shaft shoulder 8, the other end of each rotating shaft 801 is arranged in a bearing seat 703 arranged at the top of the corresponding support frame 702, one rotating shaft 801 is fixedly provided with a driving gear 802, the driving gear 802 is meshed with the driven gear 705 below the driving gear, the driven gear 705 is connected with the motor 704 fixedly arranged on the support frame 702 through a transmission shaft, and the motor 704 is connected with the controller 2. The rotating speed of the motor 704 is 1450r/min, the number of teeth of the driving gear 802 is 24, and the number of teeth of the driven gear 705 is 72.
The pressure maintaining cylinder 609 and the drainage cylinder 501 are made of 316 stainless steel, and the upper limit of the pressure born by the pressure maintaining cylinder 609, the drainage cylinder 501, the high-pressure camera, the high-pressure lamp, the pressure sensor, the temperature sensor, the overflow valve 604 and the high-pressure valve 603 is larger than 110MPa.
A deep sea macro organism fidelity culture method comprises the following steps:
(1) The pressurizing process comprises the following steps: before the sampling module is in butt joint with the culture module 6, seawater is injected into the culture module 6, high-pressure water is injected into the culture module 6 through a high-pressure valve 603 on a pressure pump connecting end cover 606, a pressure sensor measures the real-time pressure in the culture module 6, a pressure signal is output to a computer 1, a display of the computer 1 displays a real-time pressure value, and when the pressure reaches the requirement, the high-pressure valve 603 is closed;
(2) The refrigerating process comprises the following steps: the low-temperature water in the refrigerator 401 flows into the condenser pipe 402 through the inlet of the condenser pipe 402, so that the high-pressure seawater in the pressure maintaining cylinder 609 is cooled, the water in the condenser pipe 402 flows into the refrigerator 401 through the outlet of the condenser pipe 402, the cyclic utilization is realized, the temperature sensor measures the real-time temperature in the culture module 6, the real-time temperature signal is output to the computer 1, the display of the computer 1 displays the real-time temperature value, and the temperature in the macro biological culture module 6 is maintained at 2-4 ℃;
(3) The transfer process comprises the following steps: the sampling module is in butt joint with the culture module 6 through a butt joint hole 613 on the end cover 606, a through hole 614 on the end cover 606 is opened through a turning plate sealing mechanism 607, macro organisms in the sampling module are transferred into an inner cavity of a pressure maintaining cylinder 609 through the through hole 614, and after the macro organisms are completely transferred, the through hole 614 on the end cover 606 is closed through the turning plate sealing mechanism 607;
(4) The culture process comprises the following steps: after the macro organism is completely transferred to the culture module 6, the temperature and the pressure in the pressure maintaining cylinder 609 are monitored in real time through the display of the computer 1, when the pressure is lower than a set threshold value, the input end of the controller 2 receives a real-time pressure signal from the pressure sensor, and the output end of the controller 2 outputs a control signal to control the pressurizing pump to pressurize; when the temperature is higher than the set threshold value, the input end of the controller 2 receives a real-time temperature signal from the temperature sensor, the output end of the controller 2 outputs a control signal to control the refrigeration power of the refrigerator 401, so that the temperature in the macro biological culture module 6 is maintained at 2-4 ℃; automatically delivering the baits into the pressure maintaining cylinder 609 by the automatic bait delivery device 602;
(5) And (3) drainage: after the macro-organisms are cultured in the culture module 6 for a period of time, water in the pressure maintaining cylinder 609 is replaced, the motor 704 is controlled by the controller 2 to drive the driven gear 705 to rotate, the driven gear 705 is meshed with the driving gear 802 to drive the driving gear 802 to rotate, the rotating gear drives the rotating shaft 801 to rotate, the culture module 6 is driven to rotate by 90 degrees, the culture module 6 is horizontally placed, the high-pressure ball valve 502 at the bottom of the pressure maintaining cylinder 609 is opened, water in the pressure maintaining cylinder 609 flows out into the drainage cylinder 501, and seawater in the pressure maintaining cylinder 609 is transferred in batches, so that no pressure drop transfer is realized; after the transfer is completed, the high-pressure ball valve 502 is closed, the motor 704 is controlled by the controller 2 to drive the driven gear 705 to rotate, so that the culture module 6 is driven to rotate by 90 degrees, high-pressure water is injected into the culture by the booster pump, and the steps (1) (2) (4) are continued.
Claims (4)
1. The utility model provides a deep sea macroscopical biological fidelity culture device which characterized in that: the container comprises a support adjusting module, a control module, a culture module and a refrigerating module, wherein the support adjusting module is fixedly arranged on the container, the culture module is rotatably arranged on the support adjusting module, the refrigerating module is arranged on the culture module, and the control module is respectively connected with the culture module and the refrigerating module;
The culture module comprises a pressure maintaining cylinder and an end cover, wherein the pressure maintaining cylinder is a semi-closed cylinder with an opening at one end, the opening end of the pressure maintaining cylinder is sealed by the end cover, a watertight joint II is arranged on the outer end face of the end cover, the watertight joint II is connected with the control module, a watertight joint I connected with the watertight joint II is arranged on the inner end face of the end cover, the watertight joint I is connected with a high-pressure camera, a high-pressure lamp, a pressure sensor and a temperature sensor which are arranged in the inner cavity of the pressure maintaining cylinder, a butt joint hole for butt joint with the sampling module and a through hole for a macro-organism to pass through are arranged in the center of the end cover, a flap sealing mechanism for sealing or opening the through hole is arranged in the inner cavity of the pressure maintaining cylinder, a horn mouth is arranged on the flap sealing mechanism, and a switch valve for driving the flap sealing mechanism is arranged on the end cover; the end cover is provided with a plurality of high-pressure pipe connecting holes, and the inner cavity of the pressure maintaining cylinder is connected with a pressure gauge, an overflow valve and a high-pressure valve which are arranged on the outer end surface of the end cover through the high-pressure pipe connecting holes;
The automatic bait delivery device is arranged on the end cover and is communicated with the inner cavity of the pressure maintaining cylinder through a high-pressure pipe connecting hole;
The bottom of the pressure maintaining cylinder is provided with a drainage device, the drainage device comprises a high-pressure ball valve, a drainage cylinder and a drainage valve, one end of the high-pressure ball valve is communicated with the inner cavity of the pressure maintaining cylinder, the other end of the high-pressure ball valve is communicated with one end of the drainage cylinder, and the other end of the drainage cylinder is connected with the drainage valve; the bottom of the inner cavity of the pressure maintaining cylinder is provided with a filter plate for limiting the individual size of the deep-sea submarine organisms, and the filter plate is provided with a filter hole;
The refrigerating module comprises a condensing pipe and a refrigerating machine, the condensing pipe is arranged in the inner cavity of the pressure maintaining cylinder, the side surface of the pressure maintaining cylinder is provided with a connecting hole for the outlet and the inlet of the condensing pipe, one end of the refrigerating machine is connected with the inlet of the condensing pipe, the other end of the refrigerating machine is connected with the outlet of the condensing pipe, and the refrigerating machine is connected with the control module;
The control module comprises a computer and a controller; the computer is connected with the controller, and the controller is connected with the watertight joint II; the display of the computer comprises a temperature curve, a pressure curve, a real-time temperature and pressure data display window, and the control buttons of the computer comprise a temperature threshold setting button, a pressure threshold setting button and a data real-time storage button;
The support adjusting module comprises a base, support frames, a motor and driven gears, wherein the base is arranged on a container, two support frames are symmetrically arranged on two sides of the base, two shaft shoulders are symmetrically arranged on the side faces of the pressure maintaining cylinder, each shaft shoulder is provided with a rotating shaft, one end of each rotating shaft is arranged on the corresponding shaft shoulder, the other end of each rotating shaft is arranged in a bearing seat positioned at the top of the corresponding support frame, which is positioned on the corresponding side, through a bearing, one rotating shaft is fixedly provided with a driving gear, the driving gear is meshed with the driven gear positioned below the driving gear, the driven gear is connected with the motor fixedly arranged on the support frame through a transmission shaft, and the motor is connected with the controller.
2. The deep sea macrobiosis fidelity culture device of claim 1, wherein: the pressure maintaining cylinder and the drainage cylinder are made of 316 stainless steel, and the upper limit of the pressure born by the pressure maintaining cylinder, the drainage cylinder, the high-pressure camera, the high-pressure lamp, the pressure sensor, the temperature sensor, the overflow valve and the high-pressure valve is larger than 110MPa.
3. The deep sea macrobiosis fidelity culture device of claim 1, wherein: the motor rotating speed is 1450r/min, the number of teeth of the driving gear is 24, and the number of teeth of the driven gear is 72.
4. A deep sea macrobiosafety cultivation method based on the deep sea macrobiosafety cultivation device of any one of claims 1-3, comprising the steps of:
(1) The pressurizing process comprises the following steps: before the sampling module is in butt joint with the culture module, injecting seawater into the culture module, injecting high-pressure water into the culture module through a high-pressure valve on a connecting end cover of a booster pump, measuring the real-time pressure in the culture module by a pressure sensor, outputting a pressure signal to a computer, displaying a real-time pressure value by a display of the computer, and closing the high-pressure valve when the pressure reaches the requirement;
(2) The refrigerating process comprises the following steps: the low-temperature water in the refrigerator flows into the condenser pipe through the condenser pipe inlet, so that the high-pressure seawater in the pressure maintaining cylinder is cooled, the water in the condenser pipe flows into the refrigerator through the condenser pipe outlet, the cyclic utilization is realized, the temperature sensor measures the real-time temperature in the culture module, the real-time temperature signal is output to the computer, the display of the computer displays the real-time temperature value, and the temperature in the macro biological culture module is maintained at 2-4 ℃;
(3) The transfer process comprises the following steps: the sampling module is in butt joint with the culture module through a butt joint hole on the end cover, a through hole on the end cover is opened through the turning plate sealing mechanism, macro organisms in the sampling module are transferred into the inner cavity of the pressure maintaining cylinder through the through hole, and after the macro organisms are completely transferred, the macro organisms pass through the through hole on the closed end cover of the turning plate sealing mechanism;
(4) The culture process comprises the following steps: after the macro organism is completely transferred to the culture module, the temperature and the pressure in the pressure maintaining cylinder are monitored in real time through a display of a computer, when the pressure is lower than a set threshold value, the input end of the controller receives a real-time pressure signal from the pressure sensor, and the output end of the controller outputs a control signal to control the pressurizing pump to pressurize; when the temperature is higher than a set threshold value, the input end of the controller receives a real-time temperature signal from the temperature sensor, the output end of the controller outputs a control signal to control the refrigeration power of the refrigerator, so that the temperature in the macro biological culture module is maintained at 2-4 ℃; automatically delivering the baits into the pressure maintaining cylinder through the automatic bait delivery device;
(5) And (3) drainage: after macroorganisms are cultured in the culture module for 5-7 days, water in the pressure maintaining cylinder is replaced, a motor is controlled by a controller to drive a driven gear to rotate, the driven gear is meshed with the driving gear to drive the driving gear to rotate, and the rotating gear drives a rotating shaft to rotate, so that the culture module is driven to rotate by 90 degrees, the culture module is horizontally placed, a high-pressure ball valve at the bottom of the pressure maintaining cylinder is opened, water in the pressure maintaining cylinder flows out into a drainage cylinder, and seawater in the pressure maintaining cylinder is transferred in batches, so that no pressure drop transfer is realized; after the transfer is completed, the high-pressure ball valve is closed, the driven gear is driven to rotate by the motor controlled by the controller, so that the culture module is driven to rotate by 90 degrees, high-pressure water is injected into the culture by the booster pump, and the steps (1) and (2) and (4) are continued.
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| CN114287365B (en) * | 2022-01-25 | 2023-09-26 | 中国科学院海洋研究所 | High-pressure high-temperature-control simulation culture device for large-scale organisms in deep sea and application method of high-pressure high-temperature-control simulation culture device |
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