CN111089747A - Submarine organism fidelity sampling device and method for deep sea lander - Google Patents
Submarine organism fidelity sampling device and method for deep sea lander Download PDFInfo
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- CN111089747A CN111089747A CN202010061380.5A CN202010061380A CN111089747A CN 111089747 A CN111089747 A CN 111089747A CN 202010061380 A CN202010061380 A CN 202010061380A CN 111089747 A CN111089747 A CN 111089747A
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Abstract
The invention discloses a submarine organism fidelity sampling device for a deep sea lander, which comprises a pressure maintaining cylinder, wherein the outer wall of the pressure maintaining cylinder is provided with a pressure compensating device, a bait cylinder, a semiconductor refrigerating assembly and a circuit cylinder; an inlet sealing mechanism is arranged at the inlet of the pressure maintaining cylinder; a beard-reversing mechanism is arranged in the inner cavity of the pressure maintaining cylinder; an outlet sealing mechanism and a gear mechanism are arranged at an outlet of the pressure maintaining cylinder; the inner wall of the pressure maintaining cylinder is provided with a pressure sensor and a temperature sensor; and a power supply and a controller are arranged in the circuit barrel, and the controller is respectively connected with the semiconductor refrigeration assembly, the pressure sensor and the temperature sensor. The invention can realize the trapping and heat-preservation pressure-maintaining culture of the benthos and can effectively ensure the in-situ vital characteristics of the benthos. Meanwhile, the invention can be butted with a culture kettle, and can be used for transferring the benthos into the culture kettle in a fidelity way.
Description
Technical Field
The invention relates to a submarine organism sampling device, in particular to a submarine organism fidelity sampling device and a submarine organism fidelity sampling method for a deep sea lander.
Background
A large number of biological communities exist on the seabed, and scientific research on the benthos is an important means for human beings to know and research marine life evolution and seabed environment. The traditional benthos sampling means is trawl or non-pressure-maintaining and heat-preserving trapping devices, and the traditional benthos sampling devices cannot maintain the in-situ pressure and temperature of the deep sea bottom, so that the collected benthos die in the process of returning from the sea bottom to a mother ship on the water surface, and the living condition, the seabed environment and other precise researches of the benthos in the sea bottom area are greatly influenced. The deep sea lander is a seabed detection/observation device with simple structure, convenient operation and control and low cost, develops a submarine organism fidelity sampling device for the deep sea lander which is simple in structure, convenient to operate and reliable, obtains a submarine organism living sample, and has great significance for the research of the life science of marine organisms.
Disclosure of Invention
In order to solve the technical problems, the invention provides a submarine organism fidelity sampling device for a deep sea lander, which has a simple structure and works reliably, and provides a submarine organism fidelity sampling method for the deep sea lander.
The technical scheme for solving the problems is as follows: a submarine organism fidelity sampling device for a deep sea lander comprises a pressure maintaining cylinder, wherein a pressure compensation device, a bait cylinder, a semiconductor refrigeration assembly and a circuit cylinder are arranged on the outer wall of the pressure maintaining cylinder, the pressure compensation device is communicated with the pressure maintaining cylinder through a high-pressure pipe I, and the bait cylinder is communicated with the inner cavity of the pressure maintaining cylinder through a high-pressure pipe II and a switch valve; an inlet sealing mechanism is arranged at the inlet of the pressure maintaining cylinder, and a bell mouth is arranged at the bottom of the inlet sealing mechanism; a beard pouring mechanism for driving the benthos from the inlet of the pressure maintaining cylinder to the outlet of the pressure maintaining cylinder is arranged in the inner cavity of the pressure maintaining cylinder; an outlet sealing mechanism and a gear mechanism for controlling the opening and closing of the outlet sealing mechanism are arranged at an outlet of the pressure maintaining cylinder; the inner wall of the pressure maintaining cylinder is provided with a pressure sensor and a temperature sensor; and a power supply and a controller are arranged in the circuit barrel, the power supply supplies power for the semiconductor refrigeration assembly, the pressure sensor and the temperature sensor, and the controller is respectively connected with the semiconductor refrigeration assembly, the pressure sensor and the temperature sensor.
According to the submarine organism fidelity sampling device for the deep sea lander, the inlet sealing mechanism comprises a flap valve seat I, a flap valve cover I, a flap shaft I, a sealing ring I, a torsion spring I and a sleeve; the turning plate valve seat I is connected with the pressure maintaining cylinder in a sealing mode through a bolt I, a hinge lug is arranged on the turning plate valve seat I, a turning plate shaft I is fixed on the hinge lug, the turning plate valve cover I is hinged with the turning plate valve seat I through the turning plate shaft I and the hinge lug to open and close an inlet of the pressure maintaining cylinder, the turning plate valve cover I and the turning plate valve seat I are sealed through a sealing ring I, and a torsion spring I is further arranged on the turning plate shaft I; the sleeve is arranged on the flap valve cover I, and the pressure maintaining cylinder is provided with a trigger mechanism for limiting the sleeve and the flap valve cover I; the trigger mechanism comprises a trigger rod I, one end of the trigger rod I is provided with a trigger rope mounting hole I and is connected with the trigger rope I through the trigger rope mounting hole I, the other end of the trigger rod I penetrates through the trigger rod mounting hole I on the side wall of the pressure maintaining cylinder and then is inserted into the sleeve, the trigger rod I and the trigger rod mounting hole I are sealed through a sealing ring II, and the side wall of the pressure maintaining cylinder is provided with a limiting mechanism I for limiting the trigger rod I; the sleeve on the flap valve cover I and the central line of the trigger rod I on the trigger mechanism are on the same straight line.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the pressure compensation device comprises a pressure-resistant cylinder, a piston, a compensation device end cover and an inflation valve; the compensating device end cover is hermetically arranged at an opening at the top of the pressure-resistant cylinder; the piston is arranged in the pressure-resistant cylinder; the end cover of the compensating device is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder through a high-pressure pipe I; the bottom of the pressure-resistant cylinder is provided with a through hole II which is connected with the inflation valve.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the whisker inverting mechanism comprises a bevel gear, a rocker, a rocking handle, whiskers and a screw rod; the inverted beard is located at the inlet side of the pressure maintaining cylinder and is installed at one end of the screw rod, the bevel gear is located at the outlet side of the pressure maintaining cylinder and is installed at the other end of the screw rod, the rocking handle is arranged on the side wall of the pressure maintaining cylinder and is connected with the bevel gear through the rocker.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the bait cylinder comprises a pressure-resistant cylinder body, a bait cylinder bottom cover and a filter plate; the bait cylinder bottom cover is arranged at the bottom of the pressure-resistant cylinder body through a bolt II; bait is arranged in the pressure-resistant cylinder body; the top of the pressure-resistant cylinder body is provided with a filter plate, the filter plate is provided with filter holes, and the bait cylinder is communicated with the inner cavity of the pressure-maintaining cylinder through a high-pressure pipe II and a switch valve; the diameter of the filter holes on the filter plate is smaller than the inner diameter of the high-pressure pipe II.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the switch valve comprises an inlet interface, an outlet interface, a sealing ring III, a valve body and a trigger rod II; the utility model discloses a trigger bar II, including trigger bar II, cavity, trigger bar II, sealing washer III, I, II, two, the other ends of trigger bar are transversely inserted from the cavity opening part and are located the cavity and can slide in the cavity and be used for carrying out spacing stop gear II to the trigger bar II, the size of trigger bar and inner chamber size phase-match, it is sealed through sealing washer III between II and the valve body to trigger bar, II middle parts of trigger bar are equipped with the annular groove, and the annular groove just to import interface and export interface.
Above-mentioned deep sea is benthon fidelity sampling device for lander, export sealing mechanism including turning over board disk seat II, turning over board valve gap II, sealing washer IV, torsional spring II, turning over board axle II, turn over board disk seat II and pass through sealing washer IV and pressurize section of thick bamboo sealing connection, turning over board disk seat II on be equipped with the valve opening, valve opening department is equipped with turning over board axle II, torsional spring II install on turning over board axle II, turn over board valve gap II through turning over board axle II with turn over board disk seat II articulated, and turn over board disk seat II and be the gear form in the shape of articulated department, turn over board disk seat II and pass through the gear mechanism switching.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the gear mechanism comprises an inflation interface, a gear rod accommodating cavity and an O-shaped sealing ring I, the gear rod accommodating cavity is located in the outer wall of the pressure maintaining cylinder, the inflation interface is arranged on the pressure maintaining cylinder and communicated with the gear rod accommodating cavity, the gear rod is arranged in the gear rod accommodating cavity, the outer diameter of the gear rod is matched with the inner diameter of the gear rod accommodating cavity, the gear rod can move in the gear rod accommodating cavity, one end, away from the inflation interface, of the gear rod is provided with teeth, and the teeth are meshed with the hinging part of the flap valve seat II; the gear rod and the inner wall of the accommodating cavity of the gear rod are sealed through an O-shaped sealing ring I.
The submarine organism fidelity sampling device for the deep sea lander is characterized in that the number of the semiconductor refrigeration assemblies is two, the semiconductor refrigeration assemblies are bonded with the outer wall of the pressure maintaining cylinder through heat-conducting silica gel, each semiconductor refrigeration assembly comprises a multistage semiconductor refrigeration piece, an electric wire and an electrode seat, the semiconductor refrigeration pieces are clamped by a heat-conducting plate and a heat-radiating fin, and heat-conducting silicone grease is coated between contact surfaces; the cold end of the semiconductor refrigeration piece is connected with the outer wall of the pressure maintaining cylinder through a heat conducting plate, and the hot end of the semiconductor refrigeration piece is contacted with the seawater through a radiating fin; each stage of semiconductor refrigeration piece is connected with each electrode holder through coupling nut respectively, establishes ties through the electric wire between each electrode holder, and the electric wire is connected with the circuit section of thick bamboo through watertight cable I.
A submarine organism fidelity sampling method for a deep sea lander comprises the following steps:
(1) before launching the benthos fidelity sampling device for the deep sea lander, inflating inert gas with the water depth and pressure of 0.3 time of a sampling point into a lower cavity of a piston in the pressure compensation device through an inflation valve, wherein the piston in the pressure compensation device is positioned at the top of the cavity of the pressure compensation device; the semiconductor refrigeration component is arranged on the outer wall of the pressure maintaining cylinder, a circuit is connected, a power supply is connected, and the submarine organism fidelity sampling device for the deep sea lander is fixed on the deep sea lander;
(2) opening the inlet sealing mechanism and the switch valve on the bait cylinder, closing the outlet sealing mechanism, fixing one end of a trigger rope II on a trigger rod II, wherein one end of the trigger rod II is provided with a trigger rope mounting hole II, and the other end of the trigger rope II is bound on a bearing block of the deep sea lander; one end of a trigger rope I is fixed on one end of a trigger rod I, a trigger rope mounting hole I is formed in one end of the trigger rod I, and the other end of the trigger rope I is bound on a bearing block of the deep sea lander;
(3) in the process of lowering the deep sea lander, under the action of seawater pressure, a piston of the pressure compensation device moves downwards until the pressures in a lower cavity and an upper cavity of the piston are balanced;
(4) when the benthos fidelity sampling device for the deep sea lander is lowered to the designated seabed surface, the bait in the bait cylinder flows into the pressure maintaining cylinder through the filter plate to start trapping benthos; when observing that benthos enters the pressure maintaining cylinder, the camera on the deep sea lander releases the bearing block through the releaser, triggers the trigger rod I and the trigger rod II, closes the inlet sealing mechanism and the switch valve on the bait cylinder at the same time, and floats;
(5) in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, the pressure sensor sends a pressure signal due to the reduction of the pressure of external seawater, the input end of the controller receives a real-time pressure signal from the pressure sensor, the pressure maintaining cylinder deforms in an expansion mode, at the moment, the inert gas in the lower cavity of the piston of the pressure compensation device pushes the piston to move towards the upper cavity, seawater in the upper cavity is forced to flow into the pressure maintaining cylinder through the high-pressure pipe I, and therefore the pressure loss in the pressure maintaining cylinder caused by the expansion deformation of the pressure maintaining cylinder is compensated;
in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, as the temperature of external sea water rises, the temperature sensor sends a temperature signal, the input end of the controller receives a real-time temperature signal from the temperature sensor, the output end of the controller outputs a control signal, and the working current in the semiconductor refrigeration piece is controlled to control the refrigeration power, the cold end of the semiconductor refrigeration piece enables the temperature in the pressure-holding cylinder to be always kept at the same temperature value as a sampling point through the heat conducting plate, and the hot end transfers heat to the sea water through the radiating fin;
(6) during the fidelity transfer process of the benthos for the deep sea lander, the benthos for the deep sea lander is firstly connected with a culture kettle, connected with an inflation interface in a gear mechanism through a pressure pump, and pressurized into a pressure maintaining cylinder to a pressure 0.3-0.7 MPa higher than the water depth pressure of a sampling point, so as to drive a gear rod of the gear mechanism to open an outlet sealing mechanism, and then the pressurization is stopped, and at the moment, the pressure maintaining cylinder is the same as the pressure in the culture kettle; the rocking handle of the beard inverting mechanism is rotated to drive the bevel gear to rotate, so that the beard inverting mechanism moves upwards to drive the benthos in the pressure maintaining cylinder to move towards the culture kettle, and the benthos fidelity transfer is completed.
The invention has the beneficial effects that:
(1) the invention can realize the trapping and heat-preservation pressure-maintaining culture of the benthos and can effectively ensure the in-situ vital characteristics of the benthos. Meanwhile, the invention can be butted with a culture kettle, and can be used for transferring the benthos into the culture kettle in a fidelity way.
(2) The invention adopts two groups of independent semiconductor refrigeration components, and can independently regulate in real time according to the external environment temperature of the benthos fidelity sampling device for the deep sea lander;
(3) the invention has simple and compact structure and simple and convenient operation, and can use the deep sea lander for triggering and sealing.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a structural schematic diagram of the opening state of the inlet sealing mechanism of the invention.
Fig. 3 is a schematic structural view of the inlet sealing mechanism of the present invention in a closed state.
Fig. 4 is a schematic structural diagram of the pressure compensation device of the present invention.
Fig. 5 is a schematic view of a filter plate structure in the bait cartridge of the present invention.
Fig. 6 is a schematic view of the switching valve of the present invention.
Fig. 7 is a schematic view of the outlet sealing mechanism and gear mechanism of the present invention.
Fig. 8 is a schematic structural diagram of the semiconductor refrigeration assembly of the present invention.
Fig. 9 is a block diagram of the circuit structure of the present invention.
Fig. 10 is a circuit diagram of the controller of the present invention.
FIG. 11 is a diagram illustrating a state of the present invention before sampling.
FIG. 12 is a diagram illustrating a state after sampling according to the present invention.
FIG. 13 is a state diagram of the transfer process of the present invention.
Detailed Description
The invention is further described below with reference to the figures and examples.
As shown in fig. 1, a submarine organism fidelity sampling device for a deep sea lander comprises an inlet sealing mechanism 2, a pressure maintaining cylinder 3, an outlet sealing mechanism 6, a gear mechanism 7, a beard reversing mechanism 8 and a switch valve 10, wherein a pressure compensation device 4, a bait cylinder 12, a semiconductor refrigeration component 16 and a circuit cylinder 17 are arranged on the outer wall of the pressure maintaining cylinder 3, the pressure compensation device 4 is communicated with the pressure maintaining cylinder 3 through a high-pressure pipe i 5, and the bait cylinder 12 is communicated with the inner cavity of the pressure maintaining cylinder 3 through a high-pressure pipe ii 9 and the switch valve 10; an inlet sealing mechanism 2 is arranged at an inlet of the pressure maintaining cylinder 3, and a bell mouth 1 is arranged at the bottom of the inlet sealing mechanism 2; a beard pouring mechanism 8 for driving the benthos from the inlet of the pressure maintaining cylinder 3 to the outlet of the pressure maintaining cylinder 3 is arranged in the inner cavity of the pressure maintaining cylinder 3; an outlet sealing mechanism 6 and a gear mechanism 7 for controlling the opening and closing of the outlet sealing mechanism 6 are arranged at the outlet of the pressure maintaining cylinder 3; the inner wall of the pressure maintaining cylinder 3 is provided with a pressure sensor 20 and a temperature sensor 18; a power supply and a controller are arranged in the circuit cylinder 17, the power supply supplies power for the semiconductor refrigeration assembly 16, the pressure sensor 20 and the temperature sensor 18, and the controller is respectively connected with the semiconductor refrigeration assembly 16, the pressure sensor 20 and the temperature sensor 18.
As shown in fig. 2 and 3, the inlet sealing mechanism 2 comprises a flap valve seat i 201, a flap valve cover i 202, a flap shaft i 205, a sealing ring i 206, a torsion spring i 204 and a sleeve 203; the turning plate valve seat I201 is connected with the pressure maintaining cylinder 3 in a sealing mode through a bolt I11, a hinge lug is arranged on the turning plate valve seat I201, a turning plate shaft I205 is fixed on the hinge lug, a turning plate valve cover I202 is hinged with the turning plate valve seat I201 through the turning plate shaft I205 and the hinge lug to open and close an inlet of the pressure maintaining cylinder 3, the turning plate valve cover I202 and the turning plate valve seat I201 are sealed through a sealing ring I206, and a torsion spring I204 is further arranged on the turning plate shaft I205; the sleeve 203 is arranged on the flap valve cover I202, and the pressure maintaining cylinder 3 is provided with a trigger mechanism 13 for limiting the sleeve 203 and the flap valve cover I202; the trigger mechanism 13 comprises a trigger rod I1301, one end of the trigger rod I1301 is provided with a trigger rope mounting hole I1302, the trigger rod I1302 is connected with a trigger rope I15 through the trigger rope mounting hole I1302, the other end of the trigger rod I1301 penetrates through the trigger rod mounting hole I on the side wall of the pressure maintaining cylinder 3 and then is inserted into the sleeve 203, the trigger rod I1301 and the trigger rod mounting hole I are sealed through a sealing ring II, and the side wall of the pressure maintaining cylinder 3 is provided with a limiting mechanism I for limiting the trigger rod I1301; the sleeve 203 on the flap valve cover I202 is on the same straight line with the central line of the trigger rod I1301 on the trigger mechanism 13. When the pressure-maintaining cylinder 3 is in an open state, the trigger rod I1301 is inserted into the sleeve 203, so that the flap valve cover I202 is kept in the open state, and when the pressure-maintaining cylinder needs to be closed, the trigger rod I1301 is pulled by the trigger rope I15 to move left, so that the trigger rod I1301 is separated from the sleeve 203 on the flap valve cover I202, and the flap valve cover I202 is closed under the action of the torsion spring I204 and covers the inlet of the pressure-maintaining cylinder 3.
As shown in fig. 4, the pressure compensation device 4 includes a pressure-resistant cylinder 403, a piston 402, a compensation device end cap 404 and an inflation valve 401; the compensating device end cover 404 is hermetically arranged at the opening at the top of the pressure-resistant cylinder 403; the piston 402 is placed in the pressure-resistant cylinder 403; the compensating device end cover 404 is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder 3 through a high-pressure pipe I5; the bottom of the pressure-resistant cylinder 403 is provided with a through hole II which is connected with the inflation valve 401.
The beard inverting mechanism 8 comprises a bevel gear 803, a rocker 802, a rocking handle 801, a beard inverting 804 and a screw rod 805; the inverted beard 804 is positioned at the inlet side of the pressure maintaining cylinder 3 and is installed at one end of the screw rod 805, the bevel gear 803 is positioned at the outlet side of the pressure maintaining cylinder 3 and is installed at the other end of the screw rod 805, the rocking handle 801 is arranged on the side wall of the pressure maintaining cylinder 3, and the rocking handle 801 is connected with the bevel gear 803 through the rocking bar 802.
The bait cylinder 12 comprises a pressure-resistant cylinder body 1201, a bait cylinder bottom cover 1203 and a filter plate 1202; the bait barrel bottom cover 1203 is installed at the bottom of the pressure-resistant cylinder body 1201 through a bolt II; bait is arranged in the pressure-resistant cylinder body 1201; a filter plate 1202 is arranged at the top of the pressure-resistant cylinder body 1201, as shown in fig. 5, a filter hole 1204 is formed in the filter plate 1202, and the bait cylinder 12 is communicated with the inner cavity of the pressure maintaining cylinder 3 through a high-pressure pipe II 9 and a switch valve 10; the diameter of the filter holes 1204 on the filter plate 1202 is smaller than the inner diameter of the high-pressure pipe II 9.
As shown in fig. 6, the switching valve 10 includes an inlet port 1006, an outlet port 1003, a sealing ring iii 1002, a valve body 1004, and a trigger rod ii 1001; an inlet port 1006 is formed in the lower portion of the valve body 1004, the inlet port 1006 is communicated with the bait cylinder 12 through a high-pressure pipe II 9, an outlet port 1003 is formed in the upper portion of the valve body 1004, the outlet port 1003 is communicated with the pressure-maintaining cylinder 3 through the high-pressure pipe II 9, a cavity for communicating the inlet port 1006 with the outlet port 1003 is formed in the middle of the valve body 1004, one end of the cavity is open, a trigger rope mounting hole II 1005 is formed in one end of the trigger rod II 1001, connect through triggering II 1005 of rope mounting hole and trigger II 1501, the II 1001 other end of trigger bar transversely inserts from the cavity opening part and locates in the cavity and can slide in the cavity, the cavity opening part is equipped with and is used for carrying out spacing stop gear II to II 1001 of trigger bar, II 1001 sizes of trigger bar and inner chamber size phase-match, it is sealed through III 1002 sealing washers between II 1001 of trigger bar and the valve body 1004, II 1001 middle parts of trigger bar are equipped with the annular groove, and the annular groove just is to import interface 1006 and export interface 1003. Fig. 6 is a state diagram when the switch valve 10 is opened, the inlet port 1006 and the outlet port 1003 are communicated through an annular groove in the middle of the trigger rod ii 1001, and when the switch valve 10 needs to be closed, the trigger rod ii 1001 is pulled to move to the left through the trigger rope ii 1501, so that the annular groove in the middle of the trigger rod ii 1001 is staggered with the inlet port 1006 and the outlet port 1003, and the plugging can be realized.
As shown in fig. 7, the outlet sealing mechanism 6 includes a flap valve seat ii 603, a flap valve cover ii 601, a sealing ring iv 602, a torsion spring ii 604, and a flap shaft ii 605, the flap valve seat ii 603 is connected with the pressure maintaining cylinder 3 through the sealing ring iv 602 in a sealing manner, the flap valve seat ii 603 is provided with a valve hole, the valve hole is provided with the flap shaft ii 605, the torsion spring ii 604 is mounted on the flap shaft ii 605, the flap valve cover ii 601 is hinged to the flap valve seat ii 603 through the flap shaft ii 605, the flap valve seat ii 603 is in a gear shape at the hinged position, and the flap valve seat ii 603 is opened and closed through the gear mechanism 7.
The gear mechanism 7 comprises an inflation interface 701, a gear rod 703, a gear rod accommodating cavity and an O-shaped sealing ring II 702, the gear rod accommodating cavity is positioned in the outer wall of the pressure maintaining cylinder 3, the inflation interface 701 is arranged on the pressure maintaining cylinder 3 and is communicated with the gear rod accommodating cavity, the gear rod 703 is arranged in the gear rod accommodating cavity, the outer diameter of the gear rod 703 is matched with the inner diameter of the gear rod accommodating cavity, the gear rod 703 can move in the gear rod accommodating cavity, one end of the gear rod 703, which is far away from the inflation interface 701, is provided with teeth, the teeth are meshed with the hinged part of the flap valve seat II 603, the inflation interface 701 in the gear mechanism 7 is connected through a pressure pump to pressurize the pressure maintaining cylinder 3, the gear rod 703 moves left under the action of pressure, so that the flap valve cover II 601 of the outlet sealing mechanism 6 is driven to rotate clockwise, and the outlet of the pressure; the gear rod 703 and the inner wall of the gear rod accommodating cavity are sealed by an O-shaped sealing ring II 702.
As shown in fig. 8, there are two groups of semiconductor cooling devices 16, and the two groups of semiconductor cooling devices 1616 are independently controlled and can be controlled to work as required. The semiconductor refrigeration component 16 is bonded with the outer wall of the pressure maintaining cylinder 3 through heat-conducting silica gel, the semiconductor refrigeration component 16 comprises a multistage semiconductor refrigeration piece 1607, an electric wire 1603 and an electrode seat 1601, the semiconductor refrigeration piece 1607 is clamped by a heat-conducting plate 1606 and a heat radiating fin 1605, and heat-conducting silica gel is coated between contact surfaces; the cold end of the semiconductor refrigeration piece 1607 is connected with the outer wall of the pressure maintaining cylinder 3 through a heat conducting plate 1606, and the heat conducting plate 1606 is made of a material with high heat conductivity, so that heat can be uniformly and efficiently transferred; the hot end is contacted with the seawater through a cooling fin 1605; each stage of semiconductor refrigeration piece 1607 is respectively connected with each electrode seat 1601 through coupling nut 1604, seals through O type sealing washer III, and each electrode seat 1601 establishes ties through electric wire 1603 between each, and electric wire 1603 is connected with circuit section of thick bamboo 17 through watertight cable I19.
As shown in fig. 9 and 10, the model of the main control chip of the controller is STC89C51, in fig. 10, P1.0 is used as a temperature signal inlet, and P1.1 is used as a pressure signal inlet. P2.3 as the current increase output and P2.4 as the current decrease output. The ports K1, K2 and K3 are used as the input of the keys SET, DOWN and UP, the pulse sending frequency can be manually controlled, and the pulse sending frequency is converted into corresponding pulse signals after being processed by the single chip microcomputer to control the working current of the semiconductor refrigerating sheet 1607, so that the refrigerating power is controlled, and the heat preservation effect is achieved. The actual temperature signal measured by P1.0 is compared with the temperature signal given by the single chip microcomputer, and the working current of the semiconductor refrigeration piece 1607 is adjusted through PID control.
A submarine organism fidelity sampling method for a deep sea lander comprises the following steps:
(1) before the benthos fidelity sampling device for the deep sea lander launches water, inert gas with the water depth and pressure of 0.3 times of a sampling point is inflated into a lower cavity of a piston 402 in a pressure compensation device 4 through an inflation valve 401, and at the moment, the piston 402 in the pressure compensation device 4 is positioned at the top of the cavity of the pressure compensation device 4; the semiconductor refrigeration component 16 is arranged on the outer wall of the pressure-holding cylinder 3, is connected with a circuit and is connected with a power supply, and the submarine organism fidelity sampling device for the deep sea lander is fixed on the deep sea lander;
(2) opening the switch valve 10 on the inlet sealing mechanism 2 and the bait cartridge 12, closing the outlet sealing mechanism 6, fixing one end of a trigger rope II 1501 on a trigger rope mounting hole II 1005 formed in one end of a trigger rod II 1001, and binding the other end of the trigger rope II 1005 on a bearing block of the deep sea lander; one end of a trigger rope I15 is fixed on a trigger rope mounting hole I1302 formed in one end of a trigger rod I1301, and the other end of the trigger rope I15 is bound on a bearing block of the deep sea lander;
(3) in the process of lowering the deep sea lander, under the action of seawater pressure, the piston 402 of the pressure compensation device 4 moves downwards until the pressures in the lower cavity and the upper cavity of the piston 402 are balanced;
(4) when the submarine lander is lowered to the designated submarine surface by using the submarine organism fidelity sampling device, the bait in the bait cylinder 12 flows into the pressure maintaining cylinder 3 through the filter plate 1202 to start to trap the submarine organisms; when observing that benthos enters the pressure maintaining cylinder 3, a camera on the deep sea lander releases the bearing block through the releaser, triggers the trigger rod I1301 and the trigger rod II 1001, closes the inlet sealing mechanism 2 and the switch valve 10 on the bait cylinder 12 at the same time, and floats upwards;
(5) in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, the pressure sensor 20 sends a pressure signal due to the reduction of the pressure of external seawater, the input end of the controller receives a real-time pressure signal from the pressure sensor 20, the pressure maintaining cylinder 3 expands and deforms, at the moment, the inert gas in the lower cavity of the piston 402 of the pressure compensation device 4 pushes the piston 402 to move towards the upper cavity, seawater in the upper cavity is forced to flow into the pressure maintaining cylinder 3 through the high-pressure pipe I5, and therefore the pressure loss in the pressure maintaining cylinder 3 caused by the expansion and deformation of the pressure maintaining cylinder 3 is compensated;
in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, as the temperature of external sea water rises, the temperature sensor 18 sends a temperature signal, the input end of the controller receives a real-time temperature signal from the temperature sensor 18, the output end of the controller outputs a control signal, and the working current in the semiconductor refrigeration piece 1607 is controlled to control the refrigeration power, the cold end of the semiconductor refrigeration piece 1607 enables the temperature in the pressure maintaining cylinder 3 to be always kept at the same temperature value as a sampling point through the heat conducting plate 1606, and the hot end transfers heat to the sea water through the heat radiating fins 1605;
(6) during the fidelity transfer process of the benthos for the deep sea lander, the benthos for the deep sea lander is firstly connected with the culture kettle 14, the pressure maintaining cylinder 3 is pressurized to a water depth pressure which is 0.3MPa to 0.7MPa greater than the sampling point by connecting an inflation interface 701 in the gear mechanism 7 through a pressure pump, so as to drive a gear rod 703 of the gear mechanism 7 to open an outlet sealing mechanism 6, then the pressurization is stopped, and at the moment, the pressure in the pressure maintaining cylinder 3 is the same as the pressure in the culture kettle 14; the rocking handle 801 of the beard inverting mechanism 8 is rotated to drive the bevel gear 803 to rotate, so that the beard inverting mechanism 804 moves upwards to drive the benthos in the pressure maintaining cylinder 3 to move towards the culture kettle 14, and the benthos fidelity transfer is completed.
Claims (10)
1. The utility model provides a submarine living beings fidelity sampling device for deep sea lander which characterized in that: the device comprises a pressure maintaining cylinder, wherein a pressure compensation device, a bait cylinder, a semiconductor refrigeration assembly and a circuit cylinder are arranged on the outer wall of the pressure maintaining cylinder, the pressure compensation device is communicated with the pressure maintaining cylinder through a high-pressure pipe I, and the bait cylinder is communicated with the inner cavity of the pressure maintaining cylinder through a high-pressure pipe II through a switch valve; an inlet sealing mechanism is arranged at the inlet of the pressure maintaining cylinder, and a bell mouth is arranged at the bottom of the inlet sealing mechanism; a beard pouring mechanism for driving the benthos from the inlet of the pressure maintaining cylinder to the outlet of the pressure maintaining cylinder is arranged in the inner cavity of the pressure maintaining cylinder; an outlet sealing mechanism and a gear mechanism for controlling the opening and closing of the outlet sealing mechanism are arranged at an outlet of the pressure maintaining cylinder; the inner wall of the pressure maintaining cylinder is provided with a pressure sensor and a temperature sensor; and a power supply and a controller are arranged in the circuit barrel, the power supply supplies power for the semiconductor refrigeration assembly, the pressure sensor and the temperature sensor, and the controller is respectively connected with the semiconductor refrigeration assembly, the pressure sensor and the temperature sensor.
2. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: the inlet sealing mechanism comprises a flap valve seat I, a flap valve cover I, a flap shaft I, a sealing ring I, a torsion spring I and a sleeve; the turning plate valve seat I is connected with the pressure maintaining cylinder in a sealing mode through a bolt I, a hinge lug is arranged on the turning plate valve seat I, a turning plate shaft I is fixed on the hinge lug, the turning plate valve cover I is hinged with the turning plate valve seat I through the turning plate shaft I and the hinge lug to open and close an inlet of the pressure maintaining cylinder, the turning plate valve cover I and the turning plate valve seat I are sealed through a sealing ring I, and a torsion spring I is further arranged on the turning plate shaft I; the sleeve is arranged on the flap valve cover I, and the pressure maintaining cylinder is provided with a trigger mechanism for limiting the sleeve and the flap valve cover I; the trigger mechanism comprises a trigger rod I, one end of the trigger rod I is provided with a trigger rope mounting hole I and is connected with the trigger rope I through the trigger rope mounting hole I, the other end of the trigger rod I penetrates through the trigger rod mounting hole I on the side wall of the pressure maintaining cylinder and then is inserted into the sleeve, the trigger rod I and the trigger rod mounting hole I are sealed through a sealing ring II, and the side wall of the pressure maintaining cylinder is provided with a limiting mechanism I for limiting the trigger rod I; the sleeve on the flap valve cover I and the central line of the trigger rod I on the trigger mechanism are on the same straight line.
3. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: the pressure compensation device comprises a pressure-resistant cylinder, a piston, a compensation device end cover and an inflation valve; the compensating device end cover is hermetically arranged at an opening at the top of the pressure-resistant cylinder; the piston is arranged in the pressure-resistant cylinder; the end cover of the compensating device is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder through a high-pressure pipe I; the bottom of the pressure-resistant cylinder is provided with a through hole II which is connected with the inflation valve.
4. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: the beard-inverting mechanism comprises a bevel gear, a rocker, a rocking handle, a beard inverting mechanism and a screw rod; the inverted beard is located at the inlet side of the pressure maintaining cylinder and is installed at one end of the screw rod, the bevel gear is located at the outlet side of the pressure maintaining cylinder and is installed at the other end of the screw rod, the rocking handle is arranged on the side wall of the pressure maintaining cylinder and is connected with the bevel gear through the rocker.
5. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: the bait cylinder comprises a pressure-resistant cylinder body, a bait cylinder bottom cover and a filter plate; the bait cylinder bottom cover is arranged at the bottom of the pressure-resistant cylinder body through a bolt II; bait is arranged in the pressure-resistant cylinder body; the top of the pressure-resistant cylinder body is provided with a filter plate, the filter plate is provided with filter holes, and the bait cylinder is communicated with the inner cavity of the pressure-maintaining cylinder through a high-pressure pipe II and a switch valve; the diameter of the filter holes on the filter plate is smaller than the inner diameter of the high-pressure pipe II.
6. The device for fidelity sampling of benthos for deep sea lander according to claim 5, wherein: the switch valve comprises an inlet interface, an outlet interface, a sealing ring III, a valve body and a trigger rod II; the utility model discloses a trigger bar II, including trigger bar II, cavity, trigger bar II, sealing washer III, I, II, two, the other ends of trigger bar are transversely inserted from the cavity opening part and are located the cavity and can slide in the cavity and be used for carrying out spacing stop gear II to the trigger bar II, the size of trigger bar and inner chamber size phase-match, it is sealed through sealing washer III between II and the valve body to trigger bar, II middle parts of trigger bar are equipped with the annular groove, and the annular groove just to import interface and export interface.
7. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: export sealing mechanism including turning over board disk seat II, turning over board valve gap II, sealing washer IV, torsional spring II, turning over board axle II, turn over board disk seat II and pass through sealing washer IV and pressurize section of thick bamboo sealing connection, turn over board disk seat II on be equipped with the valve opening, valve opening department is equipped with turning over board axle II, torsional spring II install on turning over board axle II, turn over board valve gap II through turning over board axle II with turn over board disk seat II articulated, and turn over board disk seat II and be the gear form in the shape of articulated department, turn over board disk seat II and pass through the gear mechanism switching.
8. The device for fidelity sampling of benthos for deep sea lander according to claim 7, wherein: the gear mechanism comprises an inflation interface, a gear rod accommodating cavity and an O-shaped sealing ring I, wherein the gear rod accommodating cavity is positioned in the outer wall of the pressure maintaining cylinder, the inflation interface is arranged on the pressure maintaining cylinder and is communicated with the gear rod accommodating cavity, the gear rod is arranged in the gear rod accommodating cavity, the outer diameter of the gear rod is matched with the inner diameter of the gear rod accommodating cavity, the gear rod can move in the gear rod accommodating cavity, one end of the gear rod, far away from the inflation interface, is provided with teeth, and the teeth are meshed with the hinging part of the flap valve seat II; the gear rod and the inner wall of the accommodating cavity of the gear rod are sealed through an O-shaped sealing ring I.
9. The device for fidelity sampling of benthos for deep sea lander according to claim 1, wherein: the semiconductor refrigeration components are adhered to the outer wall of the pressure maintaining cylinder through heat conducting silica gel, each semiconductor refrigeration component comprises a multistage semiconductor refrigeration sheet, an electric wire and an electrode seat, the semiconductor refrigeration sheets are clamped by a heat conducting plate and a heat radiating sheet, and heat conducting silica gel is coated between contact surfaces; the cold end of the semiconductor refrigeration piece is connected with the outer wall of the pressure maintaining cylinder through a heat conducting plate, and the hot end of the semiconductor refrigeration piece is contacted with the seawater through a radiating fin; each stage of semiconductor refrigeration piece is connected with each electrode holder through coupling nut respectively, establishes ties through the electric wire between each electrode holder, and the electric wire is connected with the circuit section of thick bamboo through watertight cable I.
10. A method for fidelity sampling of benthos for deep sea lander based on the device for fidelity sampling of benthos for deep sea lander of any one of claims 1 to 9, comprising the steps of:
(1) before launching the benthos fidelity sampling device for the deep sea lander, inflating inert gas with the water depth and pressure of 0.3 time of a sampling point into a lower cavity of a piston in the pressure compensation device through an inflation valve, wherein the piston in the pressure compensation device is positioned at the top of the cavity of the pressure compensation device; the semiconductor refrigeration component is arranged on the outer wall of the pressure maintaining cylinder, a circuit is connected, a power supply is connected, and the submarine organism fidelity sampling device for the deep sea lander is fixed on the deep sea lander;
(2) opening the inlet sealing mechanism and the switch valve on the bait cylinder, closing the outlet sealing mechanism, fixing one end of a trigger rope II on a trigger rod II, wherein one end of the trigger rod II is provided with a trigger rope mounting hole II, and the other end of the trigger rope II is bound on a bearing block of the deep sea lander; one end of a trigger rope I is fixed on one end of a trigger rod I, a trigger rope mounting hole I is formed in one end of the trigger rod I, and the other end of the trigger rope I is bound on a bearing block of the deep sea lander;
(3) in the process of lowering the deep sea lander, under the action of seawater pressure, a piston of the pressure compensation device moves downwards until the pressures in a lower cavity and an upper cavity of the piston are balanced;
(4) when the benthos fidelity sampling device for the deep sea lander is lowered to the designated seabed surface, the bait in the bait cylinder flows into the pressure maintaining cylinder through the filter plate to start trapping benthos; when observing that benthos enters the pressure maintaining cylinder, the camera on the deep sea lander releases the bearing block through the releaser, triggers the trigger rod I and the trigger rod II, closes the inlet sealing mechanism and the switch valve on the bait cylinder at the same time, and floats;
(5) in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, the pressure sensor sends a pressure signal due to the reduction of the pressure of external seawater, the input end of the controller receives a real-time pressure signal from the pressure sensor, the pressure maintaining cylinder deforms in an expansion mode, at the moment, the inert gas in the lower cavity of the piston of the pressure compensation device pushes the piston to move towards the upper cavity, seawater in the upper cavity is forced to flow into the pressure maintaining cylinder through the high-pressure pipe I, and therefore the pressure loss in the pressure maintaining cylinder caused by the expansion deformation of the pressure maintaining cylinder is compensated;
in the process that the benthos fidelity sampling device for the deep sea lander is recovered to the sea surface, as the temperature of external sea water rises, the temperature sensor sends a temperature signal, the input end of the controller receives a real-time temperature signal from the temperature sensor, the output end of the controller outputs a control signal, and the working current in the semiconductor refrigeration piece is controlled to control the refrigeration power, the cold end of the semiconductor refrigeration piece enables the temperature in the pressure-holding cylinder to be always kept at the same temperature value as a sampling point through the heat conducting plate, and the hot end transfers heat to the sea water through the radiating fin;
(6) during the fidelity transfer process of the benthos for the deep sea lander, the benthos for the deep sea lander is firstly connected with a culture kettle, connected with an inflation interface in a gear mechanism through a pressure pump, and pressurized into a pressure maintaining cylinder to a pressure 0.3-0.7 MPa higher than the water depth pressure of a sampling point, so as to drive a gear rod of the gear mechanism to open an outlet sealing mechanism, and then the pressurization is stopped, and at the moment, the pressure maintaining cylinder is the same as the pressure in the culture kettle; the rocking handle of the beard inverting mechanism is rotated to drive the bevel gear to rotate, so that the beard inverting mechanism moves upwards to drive the benthos in the pressure maintaining cylinder to move towards the culture kettle, and the benthos fidelity transfer is completed.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855307A (en) * | 2020-07-14 | 2020-10-30 | 上海交通大学 | Traction sampling type active pressure maintaining in-situ seawater sampler and sampling method thereof |
| CN112314509A (en) * | 2020-12-17 | 2021-02-05 | 中国科学院深海科学与工程研究所 | Deep sea macrobiology fidelity culture device and culture method |
| US20210219528A1 (en) * | 2020-01-19 | 2021-07-22 | Hunan University Of Science And Technology | Suction sampler system for in situ collection of deep-sea floor organisms and method of using same |
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2020
- 2020-01-19 CN CN202010061380.5A patent/CN111089747A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210219528A1 (en) * | 2020-01-19 | 2021-07-22 | Hunan University Of Science And Technology | Suction sampler system for in situ collection of deep-sea floor organisms and method of using same |
| US11812732B2 (en) * | 2020-01-19 | 2023-11-14 | Hunan University Of Science And Technology | Suction sampler system for in situ collection of deep-sea floor organisms and method of using same |
| CN111855307A (en) * | 2020-07-14 | 2020-10-30 | 上海交通大学 | Traction sampling type active pressure maintaining in-situ seawater sampler and sampling method thereof |
| CN111855307B (en) * | 2020-07-14 | 2023-11-07 | 上海交通大学 | Traction sampling type active pressure-maintaining in-situ seawater sampler and sampling method thereof |
| CN112314509A (en) * | 2020-12-17 | 2021-02-05 | 中国科学院深海科学与工程研究所 | Deep sea macrobiology fidelity culture device and culture method |
| CN112314509B (en) * | 2020-12-17 | 2024-05-28 | 中国科学院深海科学与工程研究所 | Deep sea macro organism fidelity culture device and culture method |
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